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JPS6475000A - Method and equipment for analyzing creatinine - Google Patents

Method and equipment for analyzing creatinine

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Publication number
JPS6475000A
JPS6475000A JP23006187A JP23006187A JPS6475000A JP S6475000 A JPS6475000 A JP S6475000A JP 23006187 A JP23006187 A JP 23006187A JP 23006187 A JP23006187 A JP 23006187A JP S6475000 A JPS6475000 A JP S6475000A
Authority
JP
Japan
Prior art keywords
sample
immobilized
enzyme
hydrogen peroxide
treated
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP23006187A
Other languages
Japanese (ja)
Inventor
Matashige Ooyabu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shimadzu Corp
Original Assignee
Shimadzu Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shimadzu Corp filed Critical Shimadzu Corp
Priority to JP23006187A priority Critical patent/JPS6475000A/en
Publication of JPS6475000A publication Critical patent/JPS6475000A/en
Pending legal-status Critical Current

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  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE:To accurately and readily determine the titled substance for diagnosis of renal disease, by treating a sample with an immobilized enzyme obtained by immobilizing plural specific enzyme groups containing creatinine and metabolism thereof as substrate and enzyme group being short of one kind of the above-mentioned enzyme groups and comparing H2O2 amount. CONSTITUTION:An amount of hydrogen peroxide produced by introducing a sample from a sample inlet 6, adding a buffer liquid fed from buffer line 3, treating the sample with an immobilized enzyme column 8 each immobilized with creatinineamidehydrase, creatinineamidinohydrase and sacrosine oxidase and mixing the treated sample with red cell reagent 1 and a luminol reagent 2 in an analyzing line 12 is detected with a chemical luminescence detector. Then in the same way the sample is treated with an immobilized enzyme column 9 wherein creatinineamidinohydrase is together immobilized and hydrogen peroxide is detected in similar treatment and creatinine in the sample is analyzed, based on the difference of hydrogen peroxide concentration in each treated liquid.
JP23006187A 1987-09-14 1987-09-14 Method and equipment for analyzing creatinine Pending JPS6475000A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP23006187A JPS6475000A (en) 1987-09-14 1987-09-14 Method and equipment for analyzing creatinine

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP23006187A JPS6475000A (en) 1987-09-14 1987-09-14 Method and equipment for analyzing creatinine

Publications (1)

Publication Number Publication Date
JPS6475000A true JPS6475000A (en) 1989-03-20

Family

ID=16901938

Family Applications (1)

Application Number Title Priority Date Filing Date
JP23006187A Pending JPS6475000A (en) 1987-09-14 1987-09-14 Method and equipment for analyzing creatinine

Country Status (1)

Country Link
JP (1) JPS6475000A (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0339096A (en) * 1989-07-04 1991-02-20 Sekisui Chem Co Ltd High-performance liquid chromatography utilizing immobilized enzyme
AT409040B (en) * 2000-08-11 2002-05-27 Roche Diagnostics Gmbh CREATININSENSOR CALIBRATION
CN104568926A (en) * 2015-01-20 2015-04-29 中国科学院长春应用化学研究所 Creatinine detection method
JP2021527194A (en) * 2019-04-05 2021-10-11 インストゥルメンテーション ラボラトリー カンパニー How to improve the calibration accuracy of the creatinine / creatine sensor

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0339096A (en) * 1989-07-04 1991-02-20 Sekisui Chem Co Ltd High-performance liquid chromatography utilizing immobilized enzyme
AT409040B (en) * 2000-08-11 2002-05-27 Roche Diagnostics Gmbh CREATININSENSOR CALIBRATION
CN104568926A (en) * 2015-01-20 2015-04-29 中国科学院长春应用化学研究所 Creatinine detection method
JP2021527194A (en) * 2019-04-05 2021-10-11 インストゥルメンテーション ラボラトリー カンパニー How to improve the calibration accuracy of the creatinine / creatine sensor
JP2021528640A (en) * 2019-04-05 2021-10-21 インストゥルメンテーション ラボラトリー カンパニー Compositions and methods for improved creatinine measurement accuracy and their use

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