JPS6459068A - Method for measuring human thyroxin binding globulin - Google Patents
Method for measuring human thyroxin binding globulinInfo
- Publication number
- JPS6459068A JPS6459068A JP21507387A JP21507387A JPS6459068A JP S6459068 A JPS6459068 A JP S6459068A JP 21507387 A JP21507387 A JP 21507387A JP 21507387 A JP21507387 A JP 21507387A JP S6459068 A JPS6459068 A JP S6459068A
- Authority
- JP
- Japan
- Prior art keywords
- antibody
- monoclonal antibody
- tbg
- binding globulin
- hybridoma
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
PURPOSE:To widen a linear area when a calibration curve is made by adopting a 2 monoclonal antibody sandwich method using an immobilized 1st antibody and 2nd antibody which is labeled by a labeling agent. CONSTITUTION:The 1st antibody which is the monoclonal antibody to recognize TBG and is immobilized and the 2nd antibody which recognizes the antigen section different from the 1st antibody and is labeled by the labeling agent are used in the method of measuring the human thyroxin binding globulin TBG in a sample by an immunological method. The monoclonal antibody is preferably obtd. by fusing the antibody genic cells of an animal immunized by TBG and myeloma cells to obtain the hybridoma which produces the monoclonal antibody to recognize TBG, then culturing this hybridoma or the cell strains derived therefrom and drawing the monoclonal antibody from the cultured matter thereof. The solid phase immune enzyme immunoassay by the sandwich method using the two antibodies is, therefore, enabled. The conjugation constant of the 1st and 2nd antibodies is preferably 10<7>-10<12>l/mol.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP21507387A JPS6459068A (en) | 1987-08-31 | 1987-08-31 | Method for measuring human thyroxin binding globulin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP21507387A JPS6459068A (en) | 1987-08-31 | 1987-08-31 | Method for measuring human thyroxin binding globulin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS6459068A true JPS6459068A (en) | 1989-03-06 |
Family
ID=16666306
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP21507387A Pending JPS6459068A (en) | 1987-08-31 | 1987-08-31 | Method for measuring human thyroxin binding globulin |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6459068A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1996018102A1 (en) * | 1994-12-07 | 1996-06-13 | Fujisawa Pharmaceutical Co., Ltd. | Method of measuring the concentration of fk506-binding protein |
-
1987
- 1987-08-31 JP JP21507387A patent/JPS6459068A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1996018102A1 (en) * | 1994-12-07 | 1996-06-13 | Fujisawa Pharmaceutical Co., Ltd. | Method of measuring the concentration of fk506-binding protein |
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