JPS6430591A - Introduction of mutagen to cell - Google Patents
Introduction of mutagen to cellInfo
- Publication number
- JPS6430591A JPS6430591A JP62183534A JP18353487A JPS6430591A JP S6430591 A JPS6430591 A JP S6430591A JP 62183534 A JP62183534 A JP 62183534A JP 18353487 A JP18353487 A JP 18353487A JP S6430591 A JPS6430591 A JP S6430591A
- Authority
- JP
- Japan
- Prior art keywords
- cell
- mutagen
- concanavalin
- liposome
- introduce
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/10—Dispersions; Emulsions
- A61K9/127—Liposomes
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
PURPOSE:To effectively introduce a mutagen into a cell, by subjecting the mutagen and the cell to cell fusion in a state wherein concanavalin A is contained in liposome. CONSTITUTION:A given buffer solution is incorporated with about 1-5mg/ml phospholipid or glyceroglycolipid and about 1-5mg/ml concanavalin A and a mutagen in a concentration corresponding to a kind thereof and subjected to ultrasonic wave by an ultrasonic oscillator under cooling with an ice bath at about 20-40W for about 1-10min to prepare liposome containing the mutagen and concanavalin A. The liposome and a plant cell of tobacco or tomato or an organism cell of yeast made into protoplast are fused by polyethylene glycol method to introduce the mutagen into the cell.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP62183534A JPS6430591A (en) | 1987-07-24 | 1987-07-24 | Introduction of mutagen to cell |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP62183534A JPS6430591A (en) | 1987-07-24 | 1987-07-24 | Introduction of mutagen to cell |
Publications (1)
Publication Number | Publication Date |
---|---|
JPS6430591A true JPS6430591A (en) | 1989-02-01 |
Family
ID=16137512
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP62183534A Pending JPS6430591A (en) | 1987-07-24 | 1987-07-24 | Introduction of mutagen to cell |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS6430591A (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS60221176A (en) * | 1984-04-04 | 1985-11-05 | ル・エール・リクイツド・ソシエテ・アノニム・プール・ル・エチユド・エ・ル・エクスプルワテシヨン・デ・プロセデ・ジエオルジエ・クロード | Controller for operation of arc gas welding or torch for cutting |
JPS61195787A (en) * | 1985-02-22 | 1986-08-30 | ル・エール・リクイツド・ソシエテ・アノニム・プール・ル・エチユド・エ・ル・エクスプルワテシヨン・デ・プロセデ・ジエオルジエ・クロード | Plasma welding or cutting torch with nozzle cartridge |
-
1987
- 1987-07-24 JP JP62183534A patent/JPS6430591A/en active Pending
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS60221176A (en) * | 1984-04-04 | 1985-11-05 | ル・エール・リクイツド・ソシエテ・アノニム・プール・ル・エチユド・エ・ル・エクスプルワテシヨン・デ・プロセデ・ジエオルジエ・クロード | Controller for operation of arc gas welding or torch for cutting |
JPS61195787A (en) * | 1985-02-22 | 1986-08-30 | ル・エール・リクイツド・ソシエテ・アノニム・プール・ル・エチユド・エ・ル・エクスプルワテシヨン・デ・プロセデ・ジエオルジエ・クロード | Plasma welding or cutting torch with nozzle cartridge |
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