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JPS6413460A - Immunoassay method using amphoteric surfactant - Google Patents

Immunoassay method using amphoteric surfactant

Info

Publication number
JPS6413460A
JPS6413460A JP16877687A JP16877687A JPS6413460A JP S6413460 A JPS6413460 A JP S6413460A JP 16877687 A JP16877687 A JP 16877687A JP 16877687 A JP16877687 A JP 16877687A JP S6413460 A JPS6413460 A JP S6413460A
Authority
JP
Japan
Prior art keywords
conjugate
amphoteric surfactant
cleaned
added
cleaning liquid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP16877687A
Other languages
Japanese (ja)
Inventor
Nobuo Oshima
Masayoshi Fukuoka
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Meidensha Corp
Meidensha Electric Manufacturing Co Ltd
Original Assignee
Meidensha Corp
Meidensha Electric Manufacturing Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Meidensha Corp, Meidensha Electric Manufacturing Co Ltd filed Critical Meidensha Corp
Priority to JP16877687A priority Critical patent/JPS6413460A/en
Publication of JPS6413460A publication Critical patent/JPS6413460A/en
Pending legal-status Critical Current

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  • Investigating Or Analysing Biological Materials (AREA)

Abstract

PURPOSE:To enhance the sensitivity of immunoassay in immunohistocyoto chemical measurement by conjugating the serum to be inspected, antibody and labeling antibody and leaning the conjugate thereof with a cleaning liquid added with an ionic amphoteric surfactant. CONSTITUTION:The cell membrane of, for example, the single-layered Langerhans island cells of the rat pancreas is utilized as the antigen. This membrane is fixed and is cleaned by a physiological salt soln. of a phosphoric acid buffer. The dilute liquid of the serum to be inspected is then added and conjugated to and with this membrane and the conjugate is cleaned with the cleaning liquid added with the ionic amphoteric surfactant. The dilute liquid of the labeling antibody is added and conjugated to and with conjugate and the conjugate is cleaned with the above-mentioned cleaning liquid. Dodecyl-M- betaine (lauryl dimethyl betaine aminoacetate), etc., are used as the ionic amphoteric surfactant. The labeling antibody is thereafter measured by a fluorescent method. Since the conjugate is cleaned by the cleaning liquid added with the ionic amphoteric surfactant, the specific staining of the labeling antibody is easily discriminated and the specimen is measured with high sensitivity.
JP16877687A 1987-07-08 1987-07-08 Immunoassay method using amphoteric surfactant Pending JPS6413460A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP16877687A JPS6413460A (en) 1987-07-08 1987-07-08 Immunoassay method using amphoteric surfactant

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP16877687A JPS6413460A (en) 1987-07-08 1987-07-08 Immunoassay method using amphoteric surfactant

Publications (1)

Publication Number Publication Date
JPS6413460A true JPS6413460A (en) 1989-01-18

Family

ID=15874250

Family Applications (1)

Application Number Title Priority Date Filing Date
JP16877687A Pending JPS6413460A (en) 1987-07-08 1987-07-08 Immunoassay method using amphoteric surfactant

Country Status (1)

Country Link
JP (1) JPS6413460A (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH01227962A (en) * 1988-02-12 1989-09-12 Eastman Kodak Co Lower alcohol sulfate cleaning liquid, test kit and measurement of immunological ligand
JPH01248061A (en) * 1988-02-12 1989-10-03 Eastman Kodak Co Washing liquid, test kit and measurement of immunological ligand
JPH01305357A (en) * 1988-04-14 1989-12-08 Eastman Kodak Co Compositon, diagnosis kit and method for detecting antibody quickly
US8326940B2 (en) 1998-08-27 2012-12-04 Ntt Docomo, Inc. Transfer device

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH01227962A (en) * 1988-02-12 1989-09-12 Eastman Kodak Co Lower alcohol sulfate cleaning liquid, test kit and measurement of immunological ligand
JPH01248061A (en) * 1988-02-12 1989-10-03 Eastman Kodak Co Washing liquid, test kit and measurement of immunological ligand
JPH01305357A (en) * 1988-04-14 1989-12-08 Eastman Kodak Co Compositon, diagnosis kit and method for detecting antibody quickly
US8326940B2 (en) 1998-08-27 2012-12-04 Ntt Docomo, Inc. Transfer device

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