JPS5759157A - Enzyme electrode - Google Patents
Enzyme electrodeInfo
- Publication number
- JPS5759157A JPS5759157A JP55134907A JP13490780A JPS5759157A JP S5759157 A JPS5759157 A JP S5759157A JP 55134907 A JP55134907 A JP 55134907A JP 13490780 A JP13490780 A JP 13490780A JP S5759157 A JPS5759157 A JP S5759157A
- Authority
- JP
- Japan
- Prior art keywords
- coenzyme
- substrate
- immobilizing
- electrode
- adsorbing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000004190 Enzymes Human genes 0.000 title abstract 4
- 108090000790 Enzymes Proteins 0.000 title abstract 4
- 239000005515 coenzyme Substances 0.000 abstract 4
- 230000003100 immobilizing effect Effects 0.000 abstract 4
- 239000000758 substrate Substances 0.000 abstract 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract 2
- BAWFJGJZGIEFAR-NNYOXOHSSA-N NAD zwitterion Chemical class NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-N 0.000 abstract 2
- 102000007698 Alcohol dehydrogenase Human genes 0.000 abstract 1
- 108010021809 Alcohol dehydrogenase Proteins 0.000 abstract 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 abstract 1
- 102000004316 Oxidoreductases Human genes 0.000 abstract 1
- 108090000854 Oxidoreductases Proteins 0.000 abstract 1
- 239000006230 acetylene black Substances 0.000 abstract 1
- 239000011230 binding agent Substances 0.000 abstract 1
- 238000004132 cross linking Methods 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 229950006238 nadide Drugs 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PURPOSE:To obtain an enzyme electrode responding quickly to a change in the concn. of substrate by adsorbing and immobilizing coenzyme on a conductive carrier, and immobilizing oxidoreductase to the resultant carrier by a cross-linking method. CONSTITUTION:A material which is conductive, has good adsorbing and holding power for coenzyme and is chemically stable, such as acetylene black, is molded under pressure as a binder, whereby an electrode substrate 1 is made. An aq. soln. of, for example, coenzyme nicotinamide adenine nucleotide (NAD) is developed on the substrate 1, after which it is dried, whereby an adsorbing and immobilizing layer 2 for coenzyme is provided. Next, an aq. soln. of oxidoreductase alcohol dehydrogenase is developed on the layer 2 and is dried, whereby an immobilizing layer 3 is formed. Thence these are caused to crosslink and react in the vapor of glutaraldehyde, whereby the enzyme electrode is obtained. This enzyme electrode is mounted in the bottom end part of a holder 7, and is immersed in buffer 8 contg. substrate (ethanol) 8, so that the analysis of good response speed is accomplished by using a device constituting a counter electrode 10, a salt bridge 9, a reference electrode 9, a potentiostat 5, and a recorder 4.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP55134907A JPS5759157A (en) | 1980-09-27 | 1980-09-27 | Enzyme electrode |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP55134907A JPS5759157A (en) | 1980-09-27 | 1980-09-27 | Enzyme electrode |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5759157A true JPS5759157A (en) | 1982-04-09 |
| JPS6243496B2 JPS6243496B2 (en) | 1987-09-14 |
Family
ID=15139310
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP55134907A Granted JPS5759157A (en) | 1980-09-27 | 1980-09-27 | Enzyme electrode |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5759157A (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02209300A (en) * | 1989-02-10 | 1990-08-20 | Takara Co Ltd | decorative body |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS54140594A (en) * | 1978-04-24 | 1979-10-31 | Matsushita Electric Ind Co Ltd | Enzyme electrode |
-
1980
- 1980-09-27 JP JP55134907A patent/JPS5759157A/en active Granted
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS54140594A (en) * | 1978-04-24 | 1979-10-31 | Matsushita Electric Ind Co Ltd | Enzyme electrode |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6243496B2 (en) | 1987-09-14 |
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