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JPS57136165A - Immunological measuring reagent - Google Patents

Immunological measuring reagent

Info

Publication number
JPS57136165A
JPS57136165A JP56022415A JP2241581A JPS57136165A JP S57136165 A JPS57136165 A JP S57136165A JP 56022415 A JP56022415 A JP 56022415A JP 2241581 A JP2241581 A JP 2241581A JP S57136165 A JPS57136165 A JP S57136165A
Authority
JP
Japan
Prior art keywords
antigen
antibody
combined
insoluble
measuring reagent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP56022415A
Other languages
Japanese (ja)
Inventor
Takashi Kudo
Toshiyuki Sugawara
Suguru Mochida
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mochida Pharmaceutical Co Ltd
Original Assignee
Mochida Pharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mochida Pharmaceutical Co Ltd filed Critical Mochida Pharmaceutical Co Ltd
Priority to JP56022415A priority Critical patent/JPS57136165A/en
Priority to CH969/82A priority patent/CH658131A5/en
Priority to SE8200978A priority patent/SE8200978L/en
Priority to IT47814/82A priority patent/IT1147626B/en
Priority to NL8200631A priority patent/NL8200631A/en
Priority to GB8204602A priority patent/GB2095831B/en
Priority to FR8202669A priority patent/FR2500166B1/en
Priority to DE3205849A priority patent/DE3205849C2/en
Publication of JPS57136165A publication Critical patent/JPS57136165A/en
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G01N33/76—Human chorionic gonadotropin including luteinising hormone, follicle stimulating hormone, thyroid stimulating hormone or their receptors

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Endocrinology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Reproductive Health (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

PURPOSE: To prepare an immunological measuring reagent which shortens a measuring time without the reduction in measuring sensitivity and precision, by a method wherein an insoluble antibody and a labeled antibody consists of a single clonal antibody combined through recognition of an antigen determining site being different in an indentical antigen.
CONSTITUTION: In an immunological measuring reagent by a sandwitch system, an insoluble antibody and labeled antibody consists of antibodies which are capable of being combined through recognition of an antigen determining site being different in an identical antigen to be assayed, and an antigen which is kept away from a near part where the combination of the one antibody with the antigen is disturbed through the combination of the other antibody with antigen, and is combined with a site being prevented from mutual interference. An enzyme, a radioactive isotope, or a fluorescent material is used as a labeling agent. Polystyrene, polyethylene, paper, glass are used as an insoluble carrier, and the insoluble carrier, formed in the shape of a knurling tool, a sphericity, a rod, a disc, and a receptacle, can be used.
COPYRIGHT: (C)1982,JPO&Japio
JP56022415A 1981-02-18 1981-02-18 Immunological measuring reagent Pending JPS57136165A (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
JP56022415A JPS57136165A (en) 1981-02-18 1981-02-18 Immunological measuring reagent
CH969/82A CH658131A5 (en) 1981-02-18 1982-02-17 REAGENT AND METHOD FOR IMMUNOLOGICAL ANALYSIS.
SE8200978A SE8200978L (en) 1981-02-18 1982-02-17 METHODS AND IMMUNOLOGICAL ANALYSIS
IT47814/82A IT1147626B (en) 1981-02-18 1982-02-17 REAGENT FOR IMMUNOLOGICAL TEST AND METHOD FOR EFFECTING THE TEST
NL8200631A NL8200631A (en) 1981-02-18 1982-02-17 REAGENT AND METHOD FOR IMMUNOLOGICAL ANALYSIS.
GB8204602A GB2095831B (en) 1981-02-18 1982-02-17 Monoclonal antibody reagent and method for immunological assay
FR8202669A FR2500166B1 (en) 1981-02-18 1982-02-18 REAGENT AND METHOD FOR IMMUNOASSAY OF ANTIGENS
DE3205849A DE3205849C2 (en) 1981-02-18 1982-02-18 Reagent for immunological determination

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP56022415A JPS57136165A (en) 1981-02-18 1981-02-18 Immunological measuring reagent

Publications (1)

Publication Number Publication Date
JPS57136165A true JPS57136165A (en) 1982-08-23

Family

ID=12082025

Family Applications (1)

Application Number Title Priority Date Filing Date
JP56022415A Pending JPS57136165A (en) 1981-02-18 1981-02-18 Immunological measuring reagent

Country Status (8)

Country Link
JP (1) JPS57136165A (en)
CH (1) CH658131A5 (en)
DE (1) DE3205849C2 (en)
FR (1) FR2500166B1 (en)
GB (1) GB2095831B (en)
IT (1) IT1147626B (en)
NL (1) NL8200631A (en)
SE (1) SE8200978L (en)

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6060557A (en) * 1983-09-13 1985-04-08 Eisai Co Ltd Method and reagent for measuring pivka-ii
JPS6080768A (en) * 1983-10-12 1985-05-08 Eisai Co Ltd Method and reagent for measuring basic fetal protein
JPS60231168A (en) * 1984-05-01 1985-11-16 Teijin Ltd Immunoassay reagent and kit employing monoclonal antibody for human alpha2-plasmin inhibitor
JPS6134465A (en) * 1984-07-26 1986-02-18 Teijin Ltd Immunoassay reagent and kit using monoclonal antibody against human alpha2-plasmin inhibitor
JPS61196166A (en) * 1985-02-27 1986-08-30 Eiken Kagaku Kk Imunological measurement reagent and preparation thereof
JPS61213670A (en) * 1985-03-20 1986-09-22 Teijin Ltd Method for measuring human alpha2-plasmin inhibitor
JPS61213671A (en) * 1985-03-20 1986-09-22 Teijin Ltd Method for measuring human plasmin-alpha2-plasmin inhibitor compound body
WO1986007154A1 (en) * 1985-05-31 1986-12-04 Teijin Limited Method for assaying human pulmonary surface active substance and reagent kit therefor
JPS6373152A (en) * 1986-09-17 1988-04-02 Dainabotsuto Kk Immunologic measurement of elastase 1 and reagent for measurement

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2118300B (en) * 1982-02-12 1985-06-19 Corning Glass Works Method of immunoassay
DK76983A (en) * 1982-03-05 1983-09-06 Takeda Chemical Industries Ltd METHOD AND REAGENT FOR IMMUNKEMIC DETERMINATION OF HUMAN CHORIOGONADOTROPIN
US4477576A (en) * 1982-07-26 1984-10-16 Mex Research Associates Antigen assay method and kit
US4606855A (en) * 1982-07-26 1986-08-19 Mex Research Associates C/O Leon Reimer Monoclonal antibody to digoxin
US4503143A (en) * 1982-08-20 1985-03-05 Btc Diagnostics Limited Partnership Enzyme immunoassay with two-part solution of tetramethylbenzidine as chromogen
JPS59155762A (en) * 1982-12-06 1984-09-04 フイ−ルダ−・ジルレスピ−・デイビス・リミテツド Field immunoassay reaction system and method thereof
AU529210B3 (en) * 1983-02-02 1983-04-14 Australian Monoclonal Development Pty. Ltd. Monoclonal antibody in occult blood diagnosis
US4508643A (en) * 1983-02-08 1985-04-02 Baxter Travenol Laboratories, Inc. Rat antibody to hCG
US4579827A (en) * 1983-03-11 1986-04-01 Sloan-Kettering Institute For Cancer Research Monoclonal antibodies to human gastrointestinal cancers and hybridoma method of production of the monoclonal antibodies
DE3323645A1 (en) * 1983-07-01 1985-01-10 Biotest-Serum-Institut Gmbh, 6000 Frankfurt DIAGNOSTIC TEST SYSTEM
US4690890A (en) * 1984-04-04 1987-09-01 Cetus Corporation Process for simultaneously detecting multiple antigens using dual sandwich immunometric assay
EP0158291A3 (en) * 1984-04-10 1988-07-27 Takeda Chemical Industries, Ltd. A method for purifying carcinoembryonic antigen and a method for producing a carcinoembryonic antigen-reactive monoclonal antibody
US5011771A (en) * 1984-04-12 1991-04-30 The General Hospital Corporation Multiepitopic immunometric assay
GR850899B (en) * 1984-04-12 1985-11-25 Gen Hospital Corp
JPS6238362A (en) * 1985-08-12 1987-02-19 Chemo Sero Therapeut Res Inst Method for measuring tsh
DE3539215A1 (en) * 1985-11-05 1987-05-07 Boehringer Mannheim Gmbh METHOD FOR DETERMINING AN IMMUNOLOGICALLY BINDABLE ANALYT
EP0223977B1 (en) * 1985-11-25 1990-10-10 Wako Pure Chemical Industries, Ltd. Colour developing method in clinical examinations
JPS63127160A (en) * 1986-06-30 1988-05-31 Sukeyasu Yoneda Detection of specific protein
JPH02213766A (en) * 1989-02-15 1990-08-24 Mochida Pharmaceut Co Ltd Reagent and implement for immunoassay
GB2270976A (en) * 1992-09-18 1994-03-30 Marconi Gec Ltd Immunoassay/separation process using an auxiliary species on a support
AUPN214095A0 (en) 1995-04-03 1995-04-27 Australian Water Technologies Pty Ltd Method for detecting microorganisms using flow cytometry

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5716355A (en) * 1980-04-25 1982-01-27 Hoffmann La Roche Immunological method

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4244940A (en) * 1978-09-05 1981-01-13 Bio-Rad Laboratories, Inc. Single-incubation two-site immunoassay
WO1982000058A1 (en) * 1980-06-20 1982-01-07 Porter P Processes and apparatus for carrying out specific binding assays
US4486530A (en) * 1980-08-04 1984-12-04 Hybritech Incorporated Immunometric assays using monoclonal antibodies
SE8006424L (en) * 1980-09-12 1982-03-13 Jolla Cancer Res Found PUT TO DETERMINE AN ANTIGEN IN SOLUTION

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5716355A (en) * 1980-04-25 1982-01-27 Hoffmann La Roche Immunological method

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6060557A (en) * 1983-09-13 1985-04-08 Eisai Co Ltd Method and reagent for measuring pivka-ii
JPH0543357B2 (en) * 1983-09-13 1993-07-01 Eisai Co Ltd
JPS6080768A (en) * 1983-10-12 1985-05-08 Eisai Co Ltd Method and reagent for measuring basic fetal protein
JPS60231168A (en) * 1984-05-01 1985-11-16 Teijin Ltd Immunoassay reagent and kit employing monoclonal antibody for human alpha2-plasmin inhibitor
JPH0441783B2 (en) * 1984-07-26 1992-07-09 Teijin Ltd
JPS6134465A (en) * 1984-07-26 1986-02-18 Teijin Ltd Immunoassay reagent and kit using monoclonal antibody against human alpha2-plasmin inhibitor
JPS61196166A (en) * 1985-02-27 1986-08-30 Eiken Kagaku Kk Imunological measurement reagent and preparation thereof
JPS61213670A (en) * 1985-03-20 1986-09-22 Teijin Ltd Method for measuring human alpha2-plasmin inhibitor
JPS61213671A (en) * 1985-03-20 1986-09-22 Teijin Ltd Method for measuring human plasmin-alpha2-plasmin inhibitor compound body
JPH0535826B2 (en) * 1985-03-20 1993-05-27 Teijin Ltd
JPH0535827B2 (en) * 1985-03-20 1993-05-27 Teijin Ltd
WO1986007154A1 (en) * 1985-05-31 1986-12-04 Teijin Limited Method for assaying human pulmonary surface active substance and reagent kit therefor
JPS6373152A (en) * 1986-09-17 1988-04-02 Dainabotsuto Kk Immunologic measurement of elastase 1 and reagent for measurement

Also Published As

Publication number Publication date
DE3205849C2 (en) 1985-06-27
IT8247814A0 (en) 1982-02-17
NL8200631A (en) 1982-09-16
DE3205849A1 (en) 1982-09-16
IT1147626B (en) 1986-11-19
SE8200978L (en) 1983-08-19
GB2095831B (en) 1985-05-30
CH658131A5 (en) 1986-10-15
GB2095831A (en) 1982-10-06
FR2500166A1 (en) 1982-08-20
FR2500166B1 (en) 1986-03-07

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