Nothing Special   »   [go: up one dir, main page]

JPS56136650A - B lymph cell sample splitting agent and sample splitter as well as sample splitting method - Google Patents

B lymph cell sample splitting agent and sample splitter as well as sample splitting method

Info

Publication number
JPS56136650A
JPS56136650A JP3907380A JP3907380A JPS56136650A JP S56136650 A JPS56136650 A JP S56136650A JP 3907380 A JP3907380 A JP 3907380A JP 3907380 A JP3907380 A JP 3907380A JP S56136650 A JPS56136650 A JP S56136650A
Authority
JP
Japan
Prior art keywords
sample
sample splitting
splitting agent
white blood
lymph
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP3907380A
Other languages
Japanese (ja)
Inventor
Tadaaki Furuta
Fushitaka Kanejima
Tsutae Akao
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Asahi Chemical Industry Co Ltd
Original Assignee
Asahi Chemical Industry Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Asahi Chemical Industry Co Ltd filed Critical Asahi Chemical Industry Co Ltd
Priority to JP3907380A priority Critical patent/JPS56136650A/en
Publication of JPS56136650A publication Critical patent/JPS56136650A/en
Pending legal-status Critical Current

Links

Landscapes

  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)

Abstract

PURPOSE: To directly sample B lymph cells easily with good purity by putting a sample splitting agent which is a granular hydrophobic solid material contg. specific equivalent of acidic functional groups into a vessel and allowing the same to contact white blood-corpuscle suspention.
CONSTITUTION: A hydrophobic solid material (e.g.; the condensate of phenol sulfonic acid, formaldehyde and phenol) cotg. 0.1W1.2m equivalent/ml acidic functional groups is formed to a granular shape whereby the sample splitting agent is prepd. This sample splitting agent 5 is put into a column 3 having an inlet 1 and outlet 2 for liquid and a filter 4. The white blood-corpsucle suspension prepd. by using a soln. contg. protein is injected into this column 3 and unstuck white blood- corpuscles are washed out with washing liquid after a fixed time and further they are washed out with washing liquid under exertion of impact or external force, whereby B lymph cell suspension is obtained. The resultant B lymph cells have no decrease in their functions in immunological function examinations for juvenilizing ability, antibody producing ability, etc., and are sufficiently usable as a specimen for measuring HLA.
COPYRIGHT: (C)1981,JPO&Japio
JP3907380A 1980-03-28 1980-03-28 B lymph cell sample splitting agent and sample splitter as well as sample splitting method Pending JPS56136650A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP3907380A JPS56136650A (en) 1980-03-28 1980-03-28 B lymph cell sample splitting agent and sample splitter as well as sample splitting method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP3907380A JPS56136650A (en) 1980-03-28 1980-03-28 B lymph cell sample splitting agent and sample splitter as well as sample splitting method

Publications (1)

Publication Number Publication Date
JPS56136650A true JPS56136650A (en) 1981-10-26

Family

ID=12542937

Family Applications (1)

Application Number Title Priority Date Filing Date
JP3907380A Pending JPS56136650A (en) 1980-03-28 1980-03-28 B lymph cell sample splitting agent and sample splitter as well as sample splitting method

Country Status (1)

Country Link
JP (1) JPS56136650A (en)

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7134165B2 (en) 2003-07-22 2006-11-14 Panasonic Corporation Of North America Bagless vacuum cleaner system
US7181804B2 (en) 2002-11-07 2007-02-27 Panasonic Corporation Of North America Removable dirt cup assembly with external filter
US7185395B2 (en) 2002-10-18 2007-03-06 Panasonic Corporation Of North America Bagless vacuum cleaner
US7185394B2 (en) 2002-11-07 2007-03-06 Panasonic Corporation Of North America Dirt cup assembly with attachable and detachable external filter holder
US7210196B2 (en) 2003-08-29 2007-05-01 Panasonic Corporation Of North America Bagless vacuum cleaner and dirt collection assembly
US7222392B2 (en) * 2000-10-03 2007-05-29 Panasonic Corporation Of North America Airflow system for bagless vacuum cleaner
US7231688B2 (en) 2002-10-18 2007-06-19 Panasonic Corporation Of North America Dirt cup for vacuum cleaner
US7260867B2 (en) 2002-10-11 2007-08-28 Panasonic Corporation Of North America Bagless dust box for vacuum cleaner
US7305735B2 (en) 2002-10-11 2007-12-11 Panasonic Corporation Of North America Vacuum cleaner equipped with dirt cup and separate filter drawer
US7343641B2 (en) 2002-10-18 2008-03-18 Panasonic Corporation Of North America Dirt collection assembly with volcanic airflow
US7357823B1 (en) 2002-11-07 2008-04-15 Panasonic Corporation Of North America Disposable filter within a removable chamber
US7640624B2 (en) 2004-04-16 2010-01-05 Panasonic Corporation Of North America Dirt cup with dump door in bottom wall and dump door actuator on top wall

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7222392B2 (en) * 2000-10-03 2007-05-29 Panasonic Corporation Of North America Airflow system for bagless vacuum cleaner
US7260867B2 (en) 2002-10-11 2007-08-28 Panasonic Corporation Of North America Bagless dust box for vacuum cleaner
US7305735B2 (en) 2002-10-11 2007-12-11 Panasonic Corporation Of North America Vacuum cleaner equipped with dirt cup and separate filter drawer
US7185395B2 (en) 2002-10-18 2007-03-06 Panasonic Corporation Of North America Bagless vacuum cleaner
US7231688B2 (en) 2002-10-18 2007-06-19 Panasonic Corporation Of North America Dirt cup for vacuum cleaner
US7343641B2 (en) 2002-10-18 2008-03-18 Panasonic Corporation Of North America Dirt collection assembly with volcanic airflow
US7181804B2 (en) 2002-11-07 2007-02-27 Panasonic Corporation Of North America Removable dirt cup assembly with external filter
US7185394B2 (en) 2002-11-07 2007-03-06 Panasonic Corporation Of North America Dirt cup assembly with attachable and detachable external filter holder
US7357823B1 (en) 2002-11-07 2008-04-15 Panasonic Corporation Of North America Disposable filter within a removable chamber
US7134165B2 (en) 2003-07-22 2006-11-14 Panasonic Corporation Of North America Bagless vacuum cleaner system
US7210196B2 (en) 2003-08-29 2007-05-01 Panasonic Corporation Of North America Bagless vacuum cleaner and dirt collection assembly
US7640624B2 (en) 2004-04-16 2010-01-05 Panasonic Corporation Of North America Dirt cup with dump door in bottom wall and dump door actuator on top wall

Similar Documents

Publication Publication Date Title
JPS56136650A (en) B lymph cell sample splitting agent and sample splitter as well as sample splitting method
IE802210L (en) Simultaneous analysis apparatus
JPS56151357A (en) Immunoassay method
JPS5770459A (en) Cell cassette
Okada Tissue-specificity in the soluble antigens in kidney microsomes
KONDO et al. Release of lipids from red cell membrane by surface-active agents
JPS55106357A (en) Filler for liquid chromatography
RU2022241C1 (en) Method of determination of activity in system peroxidase-endogenous hydrogen peroxide in blood leucocyte on smears
CA2327414A1 (en) Immunoassay of human medullasin and diagnosis of multiple sclerosis using the same
Stoner et al. Spectrophotometric Determination of Microgram Quantities of Copper in Biologic Materials
JPS5523402A (en) Manufacture of reagent for indirect red corpuscle agglutination which absorbs simultaneously heterogenetic antibody
JPS562996A (en) Isolation of 1,2-o-isopropylidene-beta-l-idurono-6,3-lactone
Lee et al. Glucose binding and transport proteins extracted from fast-growing chicken fibroblasts
JPS5694265A (en) Anti-human retinol binding protein antibody sensitized corpsucle for measuring human retinol binding protein and production thereof and method for measurement of human retinol binding protein
JPS5678598A (en) Method for measuring enzyme immunity by simultaneous determination of two components
Johnson et al. A continuous-flow micro double diffusion technique with cellulose acetate
JPS56103366A (en) Reagent for protein analysis and quantitative method of protein
JPS55101051A (en) Simple measuring method for hemoglobin glucoside in blood
JPS55101052A (en) Simple measuring method for hemoglobin glucoside in blood
SU1610436A1 (en) Method of determining cation-active and nonionogenic surfactants in solutions
JPS56120951A (en) Measuring method for blood hemoglobin
Cullum The determination of sodium laurate in sodium N-lauroylsarcosinate
SU1067396A1 (en) Wood porosity determination method
Jalinier et al. Analysis of elastic recovery by means of the Yoshida buckling test
Zhukova et al. Ion-Exchange Separation of Small Amounts of Calcium in its Determination in Titanium