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JPS5515702A - New method of measuring activity of leucine aminopeptidase with new substrate - Google Patents

New method of measuring activity of leucine aminopeptidase with new substrate

Info

Publication number
JPS5515702A
JPS5515702A JP8670578A JP8670578A JPS5515702A JP S5515702 A JPS5515702 A JP S5515702A JP 8670578 A JP8670578 A JP 8670578A JP 8670578 A JP8670578 A JP 8670578A JP S5515702 A JPS5515702 A JP S5515702A
Authority
JP
Japan
Prior art keywords
substrate
derivative
new
activity
leucine aminopeptidase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP8670578A
Other languages
Japanese (ja)
Inventor
Yasuo Fujii
Kazutoshi Shimamoto
Tadami Akatsuka
Osamu Kodama
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
YATORON KK
Mitsubishi Kagaku Iatron Inc
Original Assignee
YATORON KK
Mitsubishi Kagaku Iatron Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by YATORON KK, Mitsubishi Kagaku Iatron Inc filed Critical YATORON KK
Priority to JP8670578A priority Critical patent/JPS5515702A/en
Publication of JPS5515702A publication Critical patent/JPS5515702A/en
Pending legal-status Critical Current

Links

Landscapes

  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE: Using a new substrate of anilide derivative is measured the activity of leucine aminopeptidase in samples from living bodies, which is required for diangoses of acute hepatitis, hepatic cancer, metastatic hepatic cancer, hepato- cirrhosis, cholangia, etc.
CONSTITUTION: A derivative of L-leucyl-4-hydroxyanilide of the formula is used as the substrate and the 4-hydroxyaniline derivative formed by the action of LAP is condensed through oxidation to form a colored compound, which is colorimetrically determined, whereby the activity of LAP in samples from living bodies can be measured. The L-leucy 1-4-hydroxyanilide derivative, the substrate, is obtained by reacting usually activated L-leucine having protected amino group with p-hydroxyaniline derivative followed by removing the protecting group from the condensation product. As shown in the table, the substrate in the present invention is greatly excellent in substrate reactivity compared to conventional substrates.
COPYRIGHT: (C)1980,JPO&Japio
JP8670578A 1978-07-18 1978-07-18 New method of measuring activity of leucine aminopeptidase with new substrate Pending JPS5515702A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP8670578A JPS5515702A (en) 1978-07-18 1978-07-18 New method of measuring activity of leucine aminopeptidase with new substrate

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP8670578A JPS5515702A (en) 1978-07-18 1978-07-18 New method of measuring activity of leucine aminopeptidase with new substrate

Publications (1)

Publication Number Publication Date
JPS5515702A true JPS5515702A (en) 1980-02-04

Family

ID=13894336

Family Applications (1)

Application Number Title Priority Date Filing Date
JP8670578A Pending JPS5515702A (en) 1978-07-18 1978-07-18 New method of measuring activity of leucine aminopeptidase with new substrate

Country Status (1)

Country Link
JP (1) JPS5515702A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4520100A (en) * 1981-09-28 1985-05-28 Nitto Boseki Co. Ltd. Method for measuring thrombin

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS54157691A (en) * 1978-06-01 1979-12-12 Nitto Boseki Co Ltd Novel substrate for measuring enzyme activity

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS54157691A (en) * 1978-06-01 1979-12-12 Nitto Boseki Co Ltd Novel substrate for measuring enzyme activity

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4520100A (en) * 1981-09-28 1985-05-28 Nitto Boseki Co. Ltd. Method for measuring thrombin

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