JPH11139986A - Physiologically active composition derived from hydrolyzate of silk protein - Google Patents

Abstract

PROBLEM TO BE SOLVED: To prepare a physiologically active composition excellent in not only antioxidative activities but also anticancer promotion activities and anticarcinogenic activities, and useful for maintenance of beauty and health. SOLUTION: This physiological composition contains a peptide obtained by hydrolyzing a silk protein, and having 300-5,000 molecular weight, and a composition containing the physiological composition as an active ingredient is applied to a drink and food, a cosmetic and a pharmaceutical preparation.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001] The present invention relates to a physiologically active composition derived from silk protein. More specifically, a molecular weight of 300 to 5, which is obtained by allowing a protease to act on a silk protein.
Immunostimulatory effect consisting of 000 peptides, antioxidant effect,
The present invention relates to a bioactive composition having an excellent antitumor promoter action.

[0002]

TECHNICAL FIELD [0001] The composition of the present invention has a superior immunostimulatory activity for strengthening an immune function or restoring a reduced immune function, as well as an antioxidant activity and an antitumor promoting activity. It has effects such as.

[0003] The composition according to the present invention is extremely safe and is used in daily use such as health-conscious foods and drinks, luxury goods, feed for livestock and pets, and cosmetics (including bath agents). By adopting the form, effects such as prevention, reduction or improvement of cancer, infectious disease, autoimmune disease, allergy, arteriosclerosis, ischemic disease, cell aging, spots, freckles, etc. can be expected. In addition, it can be formulated into medical preparations such as oral medicines, topical medicines, injections / drops, suppositories, etc. to maintain the beauty and health of humans, pet animals, etc.
It is effective for improving the constitution.

[0004] Specific examples of uses for food and drinks include processed fish products such as kamaboko, chikuwa, and hampon, processed meat products such as sausage, ham, and wiener, processed agricultural products such as tofu, fried food, konjac, western confectionery, Japanese confectionery,
Bread, cake, jelly, pudding, snack, cookies,
Sweets such as gum, candy and ramune, noodles such as raw noodles, Chinese noodles, buckwheat, udon, sauces, soy sauce, dressing, mayonnaise, sauce, honey, seasonings such as powdered candy, starch syrup, curry powder, mustard powder, pepper Spices such as powder, jam, marmalade, chocolate spread, pickles, soybeans, sprinkles, processed vegetables and fruits such as canned and bottled vegetables and fruits, dairy products such as cheese, butter, yogurt, fruit juice, vegetable juice , Whey drinks, soft drinks, health teas, drinks such as medicinal liquors, and other health-friendly foods and drinks such as tablets, drinks, granules, etc., for maintaining nutrition for the purpose of nutritional supplementation (nutrition supplement), livestock Feed, pet food, etc. are targeted.

[0005] Examples of cosmetics include lotions, lotions, creams, ointments, shaving mousses, oils, packs, shampoos, rinses, treatments, hair tonics, hair styling products, hair growth agents, permanent solutions, Examples include skin care and hair care preparations such as hair dyes and body soaps, and bath preparations (any property such as liquid, powder, granule, and solid).

[0006] In addition, apart from these, sanitary articles, wet-type tissue paper and paper towels or cotton, scuffs, cuts, burns, burns, inflammation, eczema,
Various preparations for disinfection or treatment applied to pimples, acne, rough skin, etc. and gauze impregnated with it, plasters,
It can also be applied to eye care agents, inhalants, mouthwashes, gargles, dentifrices and the like.

[0007]

2. Description of the Related Art Western medicine has played a large role in the treatment of adult diseases such as cancer and stroke. However, dealing with such diseases as preventive medicine is becoming increasingly important in the aging society.

[0008] We are originally exposed to the invasion of viruses and microorganisms from the outside world, and survive in a situation in which foreign self-materials (for example, cancer, self-dead cells, etc.) appear inside. In this situation we can survive
This is because they have a mechanism to eliminate such foreign substances and protect themselves. Therefore, such a decrease or abnormality in the biological defense ability (immune ability) causes various diseases.

In general, stress, infectious diseases (such as measles, influenza, and AIDS) and exposure to chemical substances are considered to be factors that lead to such a decrease in immunity, and they decrease with age.

[0010] On the other hand, in our diet, in recent years, a problem that has been regarded as a problem in health management is the effect of active oxygen generated in a living body, which is considered to be caused by various synthetic food additives and residual agricultural chemicals. The living body works to remove this harmful active oxygen by enzymes and the like, but if this removing function is reduced due to aging, even normal cells and organs may be damaged, resulting in various diseases and diseases. There is a concern that the organization may age. From these facts, the usefulness of foods containing a large amount of components having an antioxidant effect such as vitamin E and vitamin C has been pointed out.

Further, air pollution and destruction of the ozone layer, which have been taken as familiar environmental problems, are also of great interest because they can cause health damage. In particular, depletion of the ozone layer increases harmful ultraviolet radiation, causing serious problems such as spots, freckles and even skin cancer. For this reason,
In some regions, clothing that exposes the skin as little as possible, or sunglasses, is encouraged and calls for protection.

[0012]

SUMMARY OF THE INVENTION The object of the present invention has been made in view of these circumstances, and provides a bioactive composition useful for maintaining our beauty and health. It is in.

[0013]

The physiologically active composition according to the present invention can be produced from a material called silk.
That is, a specific peptide obtained from this material is used as an active ingredient, and has useful effects for maintaining beauty and health, such as an immunostimulating action, an antioxidant action, and an anticarcinogenic promoting action.

[0014]

BEST MODE FOR CARRYING OUT THE INVENTION As a raw material, a protein called silk fibroin prepared from silk can be used. The production method can be produced by decomposition directly by an acid, alkali and enzyme or by a bioreactor method. For example, it can be manufactured as follows.

The silk is first heated to 80 ° C. or higher and sterilized by heating. This is heated and dissolved in a high-concentration salt solution such as lithium bromide, magnesium nitrate, and calcium chloride.
After desalting by reverse osmosis or the like, a protease is allowed to act. Proteolytic enzymes used include trypsin, chymotrypsin, papain, pepsin, rennin, pancreatin,
Elastase, carboxypeptidase, aminopeptidase, dipeptidase, alcalase and the like can be used. For example, trypsin is acted on silk 40 at a ratio of 1 to 37 ° C. and pH 7. At that time, it is preferably aseptically decomposed over 24 hours. Then 80
After deactivating the enzyme by raising the temperature to at least ℃, centrifugation is performed to remove the precipitate, whereby a degradation product derived from the silk protein is obtained. This is powdered by spray drying, freeze drying or the like.

Further, it can be purified by a gel filtration method or an ion exchange method depending on the use. The peptide thus obtained is composed of various amino acids such as cysteine, glutamic acid, aspartic acid, lysine, arginine, glycine, alanine, leucine, proline, and has a molecular weight of 300 to 5,000.

Although the physiologically active composition according to the present invention can be used as it is, if necessary, pharmaceuticals, quasi-drugs, cosmetics, bath agents, foods and beverages as shown below. Any of the various components and additives used in the above can be arbitrarily selected and used in combination to obtain a product form which is easy to use.

[1] Various fats and oils Avocado oil, almond oil, fennel oil, perilla oil,
Olive oil, orange oil, orange luffer oil, sesame oil,
Cocoa butter, chamomile oil, carrot oil, cucumber oil, tallow, tallow fatty acid, kukuinut oil, safflower oil, shea oil, soybean oil, camellia oil, corn oil, rapeseed oil, persic oil, castor oil, cottonseed oil, peanut oil, Turtle oil, mink oil, egg yolk oil, cocoa butter, palm oil, palm kernel oil, mocro, coconut oil, tallow, lard, milk fat, or hydrogenated products of these oils (hardened oil, etc.).

[2] Waxes Beeswax, carnauba wax, whale wax, lanolin, liquid lanolin, reduced lanolin, hard lanolin, candelilla wax,
Montan wax, shellac wax, etc.

[3] Mineral oil Liquid paraffin, vaseline, paraffin, ozokeride, ceresin, microcrystalline wax, squalene, squalane, pristane and the like.

[4] Fatty acids Natural fatty acids such as lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, 12-hydroxystearic acid, undecylenic acid, tall oil, lanolin fatty acid, isononanoic acid, caproic acid , 2-ethylbutanoic acid, isopentanoic acid, 2-methylpentanoic acid, 2
-Synthetic fatty acids such as ethylhexanoic acid and isopentanoic acid.

[5] Alcohols Ethanol, isopiranol, lauryl alcohol,
Natural alcohols such as cetanol, stearyl alcohol, oleyl alcohol, lanolin alcohol, cholesterol, and phytosterol; and synthetic alcohols such as 2-hexyldecanol, isostearyl alcohol, and 2-octyldodecanol.

[6] Polyhydric alcohols Ethylene oxide, ethylene glycol, diethylene glycol, triethylene glycol, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, polyethylene glycol, propylene oxide, propylene glycol , Polypropylene glycol, 1,3-butylene glycol, glycerin, pentaerythritol, sorbitol, mannitol and the like.

[7] Esters Isopropyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, oleyl oleate, decyl oleate, octyl dodecyl myristate, hexyl decyl dimethyloctanoate, cetyl lactate, Myristyl lactate, diethyl phthalate, dibutyl phthalate, lanolin acetate, ethylene glycol monostearate, propylene glycol monostearate, propylene glycol dioleate, and the like.

[8] Metal soaps Aluminum stearate, magnesium stearate, zinc stearate, calcium stearate, zinc palmitate, magnesium myristate, zinc laurate, zinc undecylenate and the like.

[9] Gum, sugars and water-soluble polymer compounds Arabic gum, benzoin gum, dammar gum, guaiac oil, Irish moss, karaya gum, tragacanth gum,
Carob gum, quinseed, agar, casein, lactose,
Fructose, sucrose and its esters, trehalose and its derivatives, dextrin, gelatin, pectin, starch, carrageenan, carboxymethyl chitin or hydroxyalkyl (C ₂ -C ₄ ) oxide to which alkylene (C ₂ -C ₄ ) oxide such as ethylene oxide is added. _{2 to} C ₄ ) chitin or chitosan, low molecular weight chitin or chitosan, chitosan salt, sulfated chitin or chitosan, phosphorylated chitin or chitosan, alginic acid and its salt, hyaluronic acid and its salt, chondroitin sulfate and its salt, heparin, ethyl cellulose , Methylcellulose, carboxymethylcellulose, carboxyethylcellulose, sodium carboxyethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, nitrocellulose, crystalline cellulose And polyalkylene oxides such as polyethylene oxide and polypropylene oxide or a crosslinked polymer thereof, carboxyvinyl polymer, polyethyleneimine and the like.

[10] Surfactant Anionic surfactant (carboxylate, sulfonate, sulfate, phosphate), cationic surfactant (amine, quaternary ammonium salt), amphoteric surfactant : Carboxylic acid type amphoteric surfactant (amino type, betaine type), sulfate ester type amphoteric surfactant, sulfonic acid type amphoteric surfactant, phosphate ester type amphoteric surfactant, nonionic surfactant (ether type non-amphoteric surfactant) Ionic surfactant, ether ester type nonionic surfactant, ester type nonionic surfactant, block polymer type nonionic surfactant, nitrogen-containing nonionic surfactant), other surfactants (natural surfactant) Agent, protein hydrolyzate derivative,
Polymer surfactant, surfactant containing titanium and silicon,
Fluorocarbon surfactants).

[11] Various vitamins Vitamin A group: retinol, retinal (vitamin A ₁ ), dehydroretinal (vitamin A ₂ ), carotene, lycopene (provitamin A), vitamin B group: thiamine hydrochloride, thiamine sulfate (Vitamin B ₁ ), riboflavin (vitamin B ₂ ), pyridoxine (vitamin B ₆ ), cyanocobalamin (vitamin B ₁₂ ), folic acids,
Nicotinic acids, pantothenic acids, biotins, choline,
Inositols, vitamin C group: ascorbic acid and its derivatives, vitamin D group: ergocalciferol (vitamin D ₂ ), cholecalciferol (vitamin D ₃ ),
Dihydrotaxerol, vitamin E group: tocopherol and its derivatives, ubiquinones, vitamin K group: phytonadione (vitamin K ₁ ), menaquinone (vitamin K ₂ ), menadione (vitamin K ₃ ), menadiol (vitamin K ₄ ), others, Essential fatty acids (vitamin F), carnitine, ferulic acid, γ-oryzanol, orotic acid,
Vitamin Ps (rutin, eriocitrin, hesperidin), vitamin U and the like.

[12] Various amino acids Valine, leucine, isoleucine, threonine, methionine, phenylalanine, tryptophan, lysine, glycine, alanine, asparagine, glutamine, serine, cysteine, cystine, tyrosine, proline, hydroxyproline, aspartic acid, glutamic acid , Hydroxylysine, arginine, ornithine, histidine, and the like, and their sulfates, phosphates, nitrates, citrates, and amino acid derivatives such as pyrrolidone carboxylic acid.

[13] Various additives derived from plant or animal raw materials These are usually processed according to the type and form of the product to be added (for example, pulverization, milling, washing, hydrolysis, Fermentation, purification, squeezing, extraction, fractionation, filtration, drying, pulverization, granulation, dissolution, sterilization, pH adjustment, deodorization, decolorization, etc. You can choose to serve. The solvent to be used for the extraction may be selected in consideration of the purpose and type of the product to be provided, or the processing to be performed later. However, usually, water and a water-soluble organic solvent (eg, ethanol, propylene glycol) are used. , 1,3-butylene glycol etc.)
It is desirable to use one kind or a mixture of two or more kinds selected from the above. However, when the content of the organic solvent is not preferable depending on the application, only water may be used, or ethanol which is easily removed after extraction may be used, and may be used alone or in an arbitrary mixture with water. Exploited extract may be used.

In addition, additives derived from plant or animal raw materials are
When used for systemic or topical external preparations or cosmetics,
Including protection of skin and hair, moisturizing, improving feel and texture, imparting flexibility, relieving irritation, relieving stress by fragrance, activating cells (preventing cell aging), suppressing inflammation,
Improvement of hair quality, prevention and improvement of rough skin, hair growth, hair growth, hair loss prevention, gloss imparting, cleaning effect, alleviation of fatigue, promotion of blood flow, warm bath effect and other cosmetic effects, as well as fragrance, deodorant,
Effects such as thickening, preservation, and buffering can also be expected.

In edible products, health and cosmetic effects such as nutritional supplementation, recovery from fatigue, tonicity, cell activation (prevention of cell aging), etc., improvement of taste, provision of color tone, aroma and gloss, stabilization, It can be used for thickening and preservation. Furthermore, in addition to these, various cosmetic and pharmaceutical effects of each known raw material are expected, and by combining these, the intended effect can be enhanced or multifunctional. It is also possible to make a product that expects an effect.

Specific plants (crude drugs) as raw materials include:
For example, Ai (Aiba), Aokazura (Seifu Fuji), Aotsuji Lafji (Kibo Mi), Ao no Agave, Acacia, Akasuguri fruit, Akagrape, Akamegashiwa (Red bud), Akane (Akane grass root), Akayanagi (John yellow), Agi (Awei) ) 、 Akinir ((Royu bark) 、 Akebi (Kidori) 、 Asa (Jin Asako) 、 Asagao
(Toshiko), Ashitaba (Asuka), Azuki (red azuki), Asenyaku (Asenyaku), Acerola, Anise fruit, Abemaki fruit,
Avocado, ama, amacha (sweet tea), amachaworu, amadogokoro (tamatake), amigasa lily (shellfish), almond (happiness peach), altea, arnica, aloe, aloe (log), aloe vera, angelica, apricot (almond jelly), ansokoko (benzone) ), Ikakozorina (bile head), Knotweed (Tiger root), Strawberry, Fig (fructose) or its leaves, Ichibai (Winter Aiko),
Ichiyakuso, Ginkgo (Ginkgo biloba, Ginkgo), Itohimemegi
(Enshi), Locust bean, Rice seed or seed coat, Inondo seed, Ibuki-kusou-so, nettle, Ylang-ylang, fennel (Fenka), Ukiyagara (Mitsuryo), Uguisukagura fruit, Turmeric (Utsukin), Usubasaishin (spicy), Usveniaoi , Japanese mulberry (Summer hay), Udo, Japanese apricot (Umeboshi) or its flesh, Vladimir, Owaurushi, Unshyukan (Chest skin), Estragon, Elephant tree (Ezo Goka), Echinashi (Hoshibamurasakikibarengiku),
Enoki, Ebisugusa (Keiko), Elderberry fruit, Elemi, Enju (Gossip, Gossip rice), Ougi (Astragalus vulgaris), Ouren (Yoshiren), Okarasuuri (Karonin), Otsudurafuji
(Self-defense), psyllium (vegetative child, vegetative grass), toucan hazel (Haruko), Obanana okera (white ajutsu), Obayaya shabushi fruit,
Great hawthorn (Yamako), Ohyisa Sanoyuki, barley
(Barley), ogurma (turning over), okra fruit, okera (white juku),
Panax ginseng (carrot), St. John's wort (brother's cut grass), Odorikosou (interrupted), Onamimi (blue ears), Onigurumi, Oninoyagara (Tenma), Onyuri (Yuri), Oninos, Halibut
(Hihayu), Ominaeshi (defeated soybean), Dutch strawberry, Dutch mustard, Dutch jelly, Dutch parsley, Dutch honeysuckle, olive, oregano, orange fruit or pericarp, Kaikei (ripe yellow), seaweed [green algae, brown algae, red Algae], cacao seeds, oysters or their leaves (persimmon), kagi kazura (chou choto), cassia, casino fruit (mushrooms), gajutsu (shadojutsu),
Kashiwa (Shaju, Shayo), Cascarilla, Kanixa (Kin Sato),
Valerian (valerian root), birch, squash, capoknoki seed, kahokusansho (shopper), cattail (bamboo), chamomile, kamiyatsude (weed), karasuri (goryu), rasabisaku (midsummer), oats, garana seeds, Karaya, Karite, Karin (Gourd), Garcinia, Kawayanagi, Kawaratake, Kawarayogi (Inchinko), Kansui (Successful), Licorice (Licorice), Kantala Asa, Candelilla, Cantou, Canna, Raspberry, Kiwi fruit, Kikarasuuri Ryone), Kikyo (Kikyo, Kikyo root), Kiku (Chrysanthemum flower), Catalpa (Azumi),
Rumex japonicus, pheasant (Kikomi), Kizuta, Kidachi aloe, Kidachikka, Kinoki, Kidahada (huangkashia), Kibanaohagi (Kokihi), Gymnema sylvesta, cabbage, cabbage immature fruit, cucumber, Kiraja saponaria, Kiraya,
Kumquat fruit, betel, quince cypress (Sengaku grass), guava fruit, guayule, Quercus infectria (gallicate), kukui nuts, wolfberry (Gugi, gugiko, gugi leaf, skeletal bark), Kusugi Kazura (Tenmen winter), Kuzu (Kuzukuzu) ), Camphor tree, gooseberry fruit, gardenia, gardenia, kunusagi, kumazasa, black pine (horsepod), clara (bitter), cranberry fruit, chestnut or its astringent skin, curculigo-latifolia fruit, watercress, grapefruit fruit , Buckthorn, black squirrel (rescue), black clover, swelling grass, clove, chlorella, mulberry (mulberry leaf, mulberry bark), kei, kaygai
(Keishi), Celosia (cow flower, rooster), Keirinsaishin
(Spicy), bay (laurel), gentian, gennoshoko (old treasured grass), cilantro fruit, koushinbara (monthly flower), sui mint, kouzo fruit, kocha (tea),
Kouhone (river bone), kouhon (straw and kara straw), koulyang, cilantro fruit, lingonberry (koshibachi), koganebana (yellow gon), kogomebayotogirisou, coconut fruit, goshuyu (goshuyu), goshoyuigo (covered bonsai), pepper (pepper),
Kopai balsam, coffee beans, kobushi, burdock
(Burdock, burdock), kobotdur, sesame (sesame), sesame clover (ginseng), garnish (five seeds), wheat (wheat), rice or rice bran (red bran, white bran), rice oil, cola acuminata seeds,
Cola and vera seeds, fenugreek fruit, colombo, kondurango, konjac, kelp, comfrey (finnari grass), sisal, sisal, saxima button dull (weird sen), cherry blossoms, leaves, fruits, bark (cherry bark), Cherries, pomegranates,
Sasa, Sasa no Yuki, Sasa Yuri (Yuri), Sazanka, Sadomodaka (Sawaka), Sweet potato, Sugar cane, Sugar beet, Sanekazura, Sanebutjujutsu (Saccharus jinju), Saffron
(Safflower, West Safflower), Pomelo fruit, Soapwort, Salmonberry fruit, Sarashinasho (Masahima), Salvia, Sawagikyo (Yamakona), Sawagulumi leaf, Sankirai (Yamakiko), Hawthorn (Yamako), Sanshinjinjin Seven ginseng), Sanshuyu (sanshuyu), Sansho (sansho), Sanzukon (Yamazone), shea, cyanoki fruit, shiitake mushroom, jiou
(Land yellow), Shion (Shi-en), Digitalis, Shikunshi (Kimiko), Shishido (Qang-act, Independent, Tang-do), Shiso-Aojiso-Chirimendiso-Katamenji-so (Shiyo-yo, Shiso-ko), Shidare-kamba (white birch skin), Rosewood, Linden, cinnamon
(Forsythia), Sesame olive (Chrysanthemum flower), Spiraea, Potato, Peony (Shakuyaku), Shajin (Sasan), Janohige
(Wheat gate winter), white oak seeds, white walnut butterfly seeds, white rhinoceros, white rhododendron dandelion, white rhododendron flowers, white minanten (southern berries), jujudama, palm fruit, ginger, ginger, shobu, iris fruit , Birch, cinchona sassilbura, cinnamon, watermelon
(Watermelon), honeysuckle (gold and silver flowers, Shinobi winter), sorrel (acidic pattern), swimberry fruit, strawberry fruit, horsetail (Qingwei), stevia, plum fruit, Scots pine cone, St. John's wort, European larch, European larch,
European walnut, Atlantic hawthorn, European dandelion, European horse chestnut, European pear fruit, European snow pear, European horseshoe, European millet foil (milfoil), European cottonberry, European horseshoe, European wildflowers, European wildflowers , Sekisho (stone iris), sage, mallow, senega, seri, celery, senkyu (river kyu), sendan,
Senna fruit or leaf, Sengin (large tate), Sengoku (drug), Souka (grass), buckwheat seed, Somenimo, Daio (daihatsu), radish, soybean, Daidai (orange peel, Kikomi), thyme, Takatodai (large) Tokugi), Takawarabi (gull spine), dark sweet cherry fruit, hollyhock, hollyhock
(Hyakurika), Tachibana (Tachibana skin), Tachiyanagi, Tamarind seeds, Onion, Tamashiba (Shinyi), Taranoki or its root bark, Tanjin (Dan ginseng), Dandelion (Bamboo British), Dammar, Cherry fruit, Chigaya or its root (Chiney) , Chicset ginseng (bamboo ginseng), chicory, cloves (cloves, cloves), ginseng (gomiko), ginseng (daisies), ginseng (Wuyi), ginseng (carrots), ginseng (mushrooms), Reimeitake (Irei), Thickweedwort, Camellia, Camellia, Spiderweed (lacquer lacustrine grass), Commelina
(Duck shark), red bean (red azuki bean), red turtle (red bean), red vine (an apricot), red ginseng (four-leaf ginseng), tsuwabuki, daico, teuchigurumi, duberry fruit, tengusa, tendaiyaku (drug), touga (winter melon) , Capsicum (Hot Pepper), Touki (Toki), Calendula, Corn or Maize Hair (Southern Hair), Spodoptera (Female Sadako), Toudondou (Ryubile), Dokudami (Juyaku), Tokon (root), Toshishi, Tochiban ginseng (Bamboo ginseng), Eucommia (Tonaka, Tonaka leaves), Tomato, Traganto, Triacanthus seed, Tormentilla, Dronoki, Troloa oi, Niseria berry fruit, Naimo (herbal medicine), Nagiikada, Naginata koju, Nazuna, Rapeseed, Natsumikan , Jujube (daijutsu), nanawaibara (gold cherry blossom), narco lily (yellow), peanut (peanut), nanten (minamitenmi), nigaki (bitter) ),
Japanese sagebrush (bitter mite), nutmeg, nikkei (cinnamon bark), leek
(Nirako), elderberry (bone grafted tree), carrot, garlic (garlic), nurde (fivefold child), leek, sapling, and thistle
(Ohi), Noibara (Fruit), Yarrow, Japanese radish (Maehu), Nobara, Nomomo, Palm, Pineapple fruit, Hibiscus, Hakata lily (Yuri), Hakama alaboshi (Bone crushing supplement), Hakusen (White moss bark), Hakuru berry Fruits, chickweeds (redheads), hazels (haruko), hasidorokoro (roth roots), basil, lotus (lotus, lotus), parsley, naked mussels, batatas,
Bee (bamboo-boiled), patchouli, mint (lightly-loaded, lightly-leaved leaves), barley (yokuinin), hanasuge (chimbo), banana, hana mint, vanilla bins, papaya, lycopodium (rat)
Habu grass, paprika, hamago (vine thorns), hamasuge (kobushi), hamabishi (situriko), hamanas (my rose flower), hamabou fu (beach storm), hamamelis, rose (rose), parietalia, harunire (yuhi, Yu white bark, yu leaf), breadfruit, hioogi (sun dried), lycopodium carrot (party), pecan nut, gangamana (stone garland, vines), cypress (extended life grass), ishi (hishomi), pistachio, beet , Hitotsuba (stone reeds), Hinata no Kozuchi (cow knees), Binankazura, Hinoki, Hiba, Castor, Sunflower, Bell pepper, Himeukikyo, Himegama (incense blossom), Himematsutake, Pimenta fruit, Juniper, Byakyou, Hiyu fruit, Biroudooi, Hirohiro Okinagusa (Hakuto Okina), Hiroha Senega (Senega), Loquat
(Loquat, loquat leaf), betel nut (large belly bark, betel locust), sprout beetle (southern wisteria), furin bussouge, butterflies (winter flowers), dandelion (winter flowers, winter leaves), fukurin agave, fujibakama (lanka), fuji bean ( Flat beans), bussouge,
Butcher bloom, grape fruit or seed coat, seeds,
Leaves, beech, Fumus cinnamon mushroom (Cordyceps), Brazilian liquorice, black currant fruit, blackberry fruit, plum fruit, furcellaria, blueberry fruit, prune, blond psyllium, bundou (green bean), loofah, safflower (safflower), henneken , Belladonna, berry fruit, boysenberry fruit, boufu (windbreak), spinach, physalis (Torone), honoki (kopoku, sumpoku), bokeh
(Gourd melon), Hosobaokera (blue ajutsu), Hosobabarengiku, Bodaiju, Button (peony), hop, jojoba, white berry fruit, Honganzu (almond jelly), Hongshimeji, Meijirutennanshaw (Tennan star), Maou (maoh), macadamia nut, Macri (sea grass), maguwa (mulberry leaf, mulberry bark), mushroom
(Bamboo boiled), matabi (mokuten tate), matsukasa, matsuba (matsuba),
Matsubusa, matsuhodo (bukuryo), mamedaoshi, marjoram, marigold, malva asagao, malvanozadin (almond ginseng), mulberry fruit, quince, marronnier, mango, mangosteen, manshugulumi, mandarin fruit, mannentake (reishi), mannanrou, mandarin orange Plant fruit (Kikomi), Mishima saiko (Saiko), Mizokashi (Hanabe lotus), Misohagi (Chisenna), Mitsugashiwa, Mitsuba, Mitsuba hamagou (Tengoshi), Midorihakka, Mimosa, Myoga,
Miracle fruit fruits, Myrrh, Myrobaran, Mukuge
(Moon Geun), Mukunoki, Mukuroji, Murasaki (purple root), Murasaki corn, Mehajiki (mother grass), Mebuki, Melaleuca, Melissa, Melilote, Melon fruit, Mokotandanpopo, Mokomyogi, Mosouchikku, Magnolia (spice),
Mokko (mokuko), maple blossoms, peach fruits and seeds
(Peach kernels) or leaves (peach leaves), sprouts, morello cherry fruits, moroheiya (burlap), yakawamurasakiimo, yakuchi (masuchi),
Cornflower, cornflower, cornflower mint, yashabushi (arrow wheel), yachiyanagi, yatsude (octagonal gold plate), mistletoe (willow parasitism), willow leaf, yabugarashi, yabukouji
(Purple gold beef), pokeweed (land), yamanoimo (yakuyaku), yamanoki (yamaharu), yamamomo (yangbai skin), yamayomogi, eucalyptus, yucca brevifolia, yukinoshita (tiger ear grass),
Yuzu fruit, lily, mugwort (Aiba), yogurusa, lime fruit, rye, peanut, lakanka fruit, raspberry leaf
Fruits, lavender, longan (Ryugan meat), Ryokucha
(Green tea), apple fruit, gentian, rubas suavisimus
(Sweet), lettuce, red currant fruit, lemon fruit,
Lemongrass, forsythia, astragalus, wax palm, loganberry fruit, logwood, rosehip,
There are Rosemary, Roselle, Roman chamomile, Wakame, Wasabi and Waremokou.

Materials derived from animal raw materials include cockscomb extract, bovine / human placenta extract, swine / cattle stomach, duodenum or intestinal extract or its decomposition product, and swine / cattle spleen extract. Or a hydrolyzate thereof, an extract of a pig or bovine brain cell or a hydrolyzate thereof, a collagen derivative such as a water-soluble collagen, an acylated collagen, a collagen hydrolyzate, an elastin, an elastin hydrolyzate, a water-soluble elastin derivative, keratin and Degradation products, silk proteins and their degradation products, porcine and bovine blood cell protein degradation products (globin peptides),
Pig / bovine hemoglobin degradation products (hemin, hematin, heme,
Protohem, heme iron, etc.), casein hydrolyzate, chicken eggs, fish meat and its hydrolyzate.

[14] Microbial culture metabolites Yeast metabolites, yeast extract, rice ferment extract, rice bran ferment extract, euglena extract, lactic acid fermentation product of skim milk powder, trehalose or derivatives thereof.

[15] α-hydroxy acid Glycolic acid, citric acid, malic acid, tartaric acid, lactic acid and the like.

[16] Inorganic pigments Silicic anhydride, magnesium silicate, talc, kaolin,
Bentonite, mica, titanium mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide,
Titanium oxide, calcium carbonate, magnesium carbonate, yellow iron oxide, red iron oxide, black iron oxide, gunjou, chromium oxide,
Chromium hydroxide, carbon black, calamine, etc.

[17] UV absorber / blocker p-aminobenzoic acid derivative, salicylic acid derivative, anthranilic acid derivative, coumarin derivative, amino acid compound,
Benzotriazole derivatives, tetrazole derivatives, imidazoline derivatives, pyrimidine derivatives, dioxane derivatives, camphor derivatives, furan derivatives, pyrone derivatives,
Nucleic acid derivatives, allantoin derivatives, nicotinic acid derivatives,
Vitamin B ₆ derivatives, oxybenzone, benzophenone, arbutin, guaiazulene, shikonin, baicalin, baicalein, berberine, neoheliopan, urocanic acid derivatives, escalol, zinc oxide, talc, kaolin and the like.

[18] Tyrosinase activity inhibitor Ascorbic acid or a derivative thereof, hydroquinone or a glycoside thereof, kojic acid or a derivative thereof, placenta extract, silk peptide, plant extract (mulberry, corn, waremoko,
Clara, mugwort, honeysuckle, yellowfin, dokudami, matsuhodo, adlay barley, odorisium, hops, hawthorn, eucalyptus, achillea millefolium, artea, keihi, mankeishi, hamamelis, yamaguwa, prolonged life grass, bellflower, toshishi, maizeko, sun dried, hemp yellow, (Sengyu, Dokkatsu, Psycho, Bohu, Hamabofu, Ogon, Peony, Peony, Genenoshoko, Kakkon, Licorice, Gopiko, Aloe, Shouma, Safflower, Green Tea, Tea, Asenyaku).

[19] Reduction / decomposition of melanin pigment Hydroquinone monobenzyl ether, phenylmercury hexachlorophene, mercuric oxide, mercurous chloride, aqueous hydrogen peroxide, zinc peroxide and the like.

[20] Action of promoting turnover / cell activation Hydroquinone, lactic acid bacteria extract, placenta extract, reishi extract, vitamin A, vitamin E, allantoin, spleen extract, thymus extract, yeast extract, fermented milk extract, plant extract ( Aloe, ogon, horsetail, gentian, burdock,
Sicon, carrot, hamamelis, hop, yokunin,
Odikoso, Assemble, Touki, Toukensenka, Amacha, Hypericum, Cucumber, Periwinkle,
Rosemary, parsley, etc.).

[21] Astringent Lactic acid, tartaric acid, succinic acid, citric acid, allantoin, zinc chloride, zinc sulfate, zinc oxide, calamine, zinc p-phenolsulfonate, aluminum potassium sulfate, resorcinol, ferric chloride, tannin Acids (including catechin compounds);

[22] Active oxygen scavenger SOD, catalase, glutathione peroxidase and the like.

[23] Antioxidants ascorbic acid and its salts, stearic acid esters, tocopherol and its ester derivatives, nordihydroguaseletenic acid, butylhydroxytoluene (BHT),
Butylhydroxyanisole (BHA), hydroxytyrosol, parahydroxyanisole, propyl gallate, sesamol, sesamolin, gossypol and the like.

[24] Lipid peroxide production inhibitor β-carotene, plant extract (cultured cells of sesame, amateur,
Hypericum, Hamamelis, Clove, Melissa, Emmeiso, Birch, Sage, Rosemary, Minami Minami,
Kina, age, ginkgo, green tea, etc.).

[25] Anti-inflammatory agent Ictamole, indomethacin, kaolin, salicylic acid, sodium salicylate, methyl salicylate, acetylsalicylic acid, diphenhydramine hydrochloride, d or dl-
Camphor, hydrocortisone, guaiazulene, camazulene, chlorpheniramine maleate, glycyrrhizic acid and its salts, glycyrrhetinic acid and its salts and the like.

[26] Disinfectant / Disinfectant Acrinol, sulfur, benzalkonium chloride, benzethonium chloride, methylrosaniline chloride, cresol, calcium gluconate, chlorhexidine gluconate, sulfamine, mercurochrome, lactoferrin or a hydrolyzate thereof.

[27] Humidifier Glycerin, propylene glycol, 1,3-butylene glycol, hyaluronic acid or its salt, polyethylene glycol, chondroitin sulfate or its salt, water-soluble chitin or chitosan derivative, sodium lactate and the like.

[28] Hair preparations Selenium disulfide, alkylisoquinolinium bromide solution, zinc pyrithione, biphenamine, thianthol, castal tincture, ginger tincture, capsicum tincture, quinine hydrochloride, strong aqueous ammonia, potassium bromate, bromate Sodium, thioglycolic acid, etc.

[29] Oxidizing agent Hydrogen peroxide solution, sodium persulfate, ammonium persulfate, sodium perborate, urea peroxide, sodium percarbonate, sodium tripolyphosphate, sodium bromate, potassium bromate, pyrroline peroxide Sodium acid, sodium orthophosphate, sodium silicate hydrogen peroxide adduct, sodium sulfate sodium chloride hydrogen peroxide adduct, β-tyrosinase enzyme solution, mushroom extract and the like.

[30] Dyes 5-aminoorthocresol, 2-amino-4-nitrophenol, 2-amino-5-nitrophenol, 1-
Amino-4-methylaminoanthraquinone, 3,3'-
Iminodiphenol, 2,4-diaminophenoxyethanol hydrochloride, 2,4-diaminophenol hydrochloride, toluene-2,5-diamine hydrochloride, nitroparaphenylenediamine hydrochloride, paraphenylenediamine hydrochloride, N-phenylparaphenylenediamine hydrochloride, meta-hydrochloride Phenylenediamine, orthoaminophenol, N-phenylparaphenylenediamine acetate, 1,4-diaminoanthraquinone, 2,6-diaminopyridine, 1,5-dihydroxynaphthalene, toluene-2,5-diamine, toluene
3,4-diamine, nitroparaphenylenediamine, paraaminophenol, paranitroorthophenylenediamine, paraphenylenediamine, paramethylaminophenol, piclamic acid, sodium piclamate,
N, 'N-bis (4-aminophenyl) -2,5-diamino-1, -4-quinonediimine, 5- (2-hydroxyethylamino) -2-methylphenol, N-phenylparaphenylenediamine, metaamino Phenol, metaphenylenediamine, 5-aminoorthocresol sulfate, 2-amino-5-nitrophenol sulfate, orthoaminophenol sulfate, orthochloroparaphenylenediamine sulfate, 4,4'-diaminodiphenylamine sulfate,
2,4-diaminophenol sulfate, toluene-2 sulfate,
5-diamine, nitroparaphenylenediamine sulfate, paraaminophenol sulfate, paranitroorthophenylenediamine sulfate, paranitrometaphenylenediamine sulfate, paraphenylenediamine sulfate, paramethylaminophenol sulfate, metaaminophenol sulfate, metaphenylenediamine sulfate, catechol , Diphenylamine, α
-Naphthol, hydroquinone, pyrogallol, phloroglucin, gallic acid, resorcin, tannic acid, sodium 2-hydroxy-5-nitro-2 ', 4'-diaminoazobenzozen-5'-sulfonate, hematein and the like.

[31] Fragrance Natural animal flavors such as musk, civet, castorium, ambergris, anise essential oil, angelica essential oil, ylang-ylang essential oil, iris essential oil, fennel essential oil, orange essential oil, cananga essential oil, caraway essential oil, cardamom essential oil, Guayakwood essential oil, cumin essential oil, black letter essential oil, cinnamon essential oil, cinnamon essential oil, geranium essential oil, copaiba balsam essential oil, coriandel essential oil, perilla essential oil, cedarwood essential oil, citronella essential oil, jasmine essential oil, ginger grass essential oil, cedar essential oil, spearmint essential oil, western Mentha essential oil, Great Fengxiang essential oil, Tuberose essential oil, T-shaped essential oil, Orange flower essential oil, Winter green essential oil, Truval sam essential oil, Batturi essential oil, Rose essential oil, Palmarosa essential oil, Cypress essential oil, Hiba essential oil,
Sandalwood essential oil, Petit glen essential oil, Bay essential oil, Vetiva essential oil, Bergamot essential oil, Peruvian balsam essential oil, Boad rose essential oil, Camphor essential oil, Mandarin essential oil, Eucalyptus essential oil, Lime essential oil, Lavender essential oil, Linaloe essential oil, Lemongrass essential oil, Lemon essential oil, Rosemary Vegetable flavors such as essential oils and Japanese peppermint essential oils, and other synthetic flavors.

[32] Dyes / Colorants Red cabbage dye, red rice dye, madder dye, anato dye, squid dye, turmeric dye, ende dye, krill dye, persimmon dye, caramel, gold, silver, gardenia dye, corn dye , Onion pigment, tamarind pigment, spirulina pigment, buckwheat whole plant pigment, cherry pigment, laver pigment, hibiscus pigment, grape juice pigment, marigold pigment, purple potato pigment, purple yam pigment, lac pigment, rutin, etc.

[33] Sweeteners Sugar, sweet tea, fructose, arabinose, galactose, xylose, stevia, mannose, erythritol, maltose, honey, glucose, miraculin, monelin, licorice extract and the like.

[34] Nutrition enhancer Shell calcined calcium, cyanocorabamine, yeast, wheat germ, egg yolk powder, hemicellulose, heme iron, etc.)

[35] Dairy products Milk, cheese, fresh cream, butter, margarine, milk powder, whey, condensed milk and the like.

[36] Others Moisturizing agents, hormones, sequestering agents, pH adjusters,
Chelating agents, preservatives / antibiotics, cooling agents, stabilizers, emulsifiers, animal / vegetable proteins and their degradation products, animal / vegetable polysaccharides and their degradation products, animal / vegetable glycoproteins and their degradation products, Blood flow enhancers, anti-inflammatory agents / anti-allergic agents, cell activators, keratolytic agents, wound treatment agents, foam enhancers, thickeners, oral agents, deodorants / deodorants, bitters, seasonings, enzymes, etc. Additive and synergistic effects can be expected by using them together.

Hereinafter, the present invention will be described specifically with reference to Examples, but the present invention is not limited thereto.

[0059]

EXAMPLE Production method by batch method 100 kg saturated calcium chloride solution and 20 kg of silk protein
Was put into a reaction vessel, and the temperature was raised to about 100 ° C. to dissolve. Then, the mixture was cooled to 37 ° C. and desalted with a reverse osmosis membrane.
For 24 hours with trypsin. After heating again at 90 ° C. for 1 hour to inactivate the enzyme, the precipitate was removed by centrifugation. This was freeze-dried to obtain about 10 kg of powder. Then, this was dissolved in a small amount of purified water, and five fractions were obtained by column fractionation (FIG. 1).

[Column fractionation conditions] Resin: Sephadex G-25 fine (manufactured by Pharmacia) Column size: φ2.6 cm × 91 cm Eluent: 0.4% ammonium carbonate Flow rate: 1.0 ml / min

According to a calibration curve (FIG. 2) using a known molecular weight substance, the digested silk protein was found to have a molecular weight of 300-300.
It was estimated to be 5,000.

Hereinafter, the immunostimulating action, antioxidant action and anticarcinogenic promoting action of the physiologically active composition obtained according to the present invention will be described.

[0063]

Example Evaluation of immunostimulatory action 0.5 ml of a 5% sheep erythrocyte suspension was administered intraperitoneally to a mouse, and after 4 days, the spleen was removed and a spleen cell suspension (2 × 10 ⁶⁾
Pieces / 0.2 ml). A test substance was added to the suspension, and the suspension was cultured at 37 ° C. for 24 hours, and the amount of antibody against sheep erythrocytes produced in the culture was measured. Measurement of antibody amount
450 μl of Veronal buffer was added to 50 μl of the culture supernatant, and further, 500 μl of 1.7% sheep erythrocyte solution and 1/25 diluted complement solution were added, and the mixture was reacted at 37 ° C. for 1 hour. As a result, hemolysis occurred in proportion to the amount of the antibody present in the culture supernatant, and the amount of hemoglobin generated by the hemolysis was evaluated by measuring the absorbance at a wavelength of 540 nm. The results are shown as values obtained by dividing the absorbance at 540 nm by the absorbance of a control to which no test substance was added. Therefore, a value larger than 1 indicates a greater effect on the antibody-producing ability. The effects on the antibody-producing ability of the silk protein hydrolyzate according to the present invention and the five fractions obtained by column fractionating the same were examined as described above, and the results are shown in Table 1.

[0064]

[Table 1]

As shown in Table 1, the silk protein hydrolyzate had an effect of increasing the antibody production ability from a low concentration, and Fr3 and Fr4 had a remarkable effect in the column fraction. Especially Fr3
Showed a strong effect 1.65 times that of the control without the test substance even at a concentration as low as 1 μg / ml. Other than these two fractions, the effect was observed, albeit weakly, and it was shown that the silk protein hydrolyzate contained many types of peptides that enhance the antibody-producing ability.

[0066]

Example: Evaluation of antioxidant activity Purified water 1 was added to black microplate (Sumilon).
20 μl of a sample that was dispensed and appropriately diluted at 00 μl / well
l, 25 μl of peroxidase manufactured by horseradish (HRP: manufactured by Tago) diluted 20,000-fold was added and stirred. Further, 25 μl of a luminescent reagent B (a luminescent reagent set: manufactured by Wako Pure Chemical Industries, Ltd.) was added and stirred again. The black microplate was set on a chemiluminescence measuring device (Luminescence Sensor JNR AB-2100: manufactured by ATTO),
The amount of luminescence was measured for 20 seconds simultaneously with the addition of 25 μl of the luminescence reagent using a pump attached to the apparatus. The chemiluminescence suppression rate was calculated by subtracting the light emission when the sample was added from the light emission when the sample was not added, and dividing that by the light emission when the sample was not added. The antioxidant activity of the silk protein hydrolyzate obtained in the present invention was evaluated as described above, and the results are shown in FIG. The 50% inhibition amount (IC ₅₀ ) was calculated from the correlation formula between the amount of silk protein degradation product used and the inhibition ratio of chemiluminescence, and the results are shown in Table 2 together with the values of the five fractions similarly obtained.

[0067]

[Table 2]

The silk protein hydrolyzate, 25.2 μg, had an action of eliminating 50% of the generated active oxygen, and Fr5, the lowest molecular weight in the column fraction, showed a strong antioxidant action of 7.3 μg. . The other fractions were also found to have antioxidant effects, albeit to varying degrees.

[0069]

[Example] Evaluation of anti-carcinogenesis promotion effect Method of H. Tokuda et al. (Cancer Lett., 40, 309 (1988))
Epstein-Barr virus early antigen (EBV
-EA) It was evaluated by an expression suppression test. In other words, radiatively infected EB virus-derived radio cells derived from a nasopharyngeal carcinoma patient activate the EB virus and produce an early antigen (EA) when a tumor promoter is acted on. By counting the number of EA-producing cells, the carcinogenesis promotion effect can be evaluated. In addition, by coexisting at this time, E by a typical oncogenic promoter, 12-O-tetradecanoylphorbol-13-acetate (TPA),
A substance that suppresses the activation of the B virus and reduces the number of EA-producing cells can be evaluated as an antitumor promoter and its action as an antitumor promoting action. The results of the evaluation of the carcinogenic promotion action of the silk protein hydrolyzate performed by the above method are shown (Table 3).

[0070]

[Table 3]

The values in the table are based on the ratio of EA-producing cells to all cells after 100 hours of cultivation with TPA (20 ng / ml) for 24 hours. Indicates the ratio. The silk protein hydrolyzate shows an anti-carcinogenic promotion effect from a low concentration, and 10 μg /
At 21.6% in ml and at 100 μg / ml cytotoxicity was observed, but completely blocked TPA-induced EB virus activation. This indicates that the silk protein hydrolyzate suppresses the carcinogenesis promotion effect of TPA, that is, is evaluated as an antitumor promoter having an anticarcinogenesis promotion effect.

[0072]

EXAMPLES Safety test 1) Primary skin irritation test A silk protein hydrolyzate according to the present invention was adjusted to an aqueous solution having a concentration of 2 w / v% each, and the back was hair-removed from a Hartley-type guinea pig (5 animals per group, body weight) (About 320 g). The determination was made 24 hours after application using the primary irritancy scoring method with erythema and edema as indices.

As a result, in all animals,
No erythema and edema were recognized, and the test was negative.

[0074]

Example: Safety test 2) Skin cumulative irritation test The silk protein hydrolyzate according to the present invention was used as solids at 2 w / v.
% Aqueous solution, and the hair of the Hartley-type guinea pig (female, 5 animals per group, body weight of about 320 g) whose hair on the abdomen was depilated 0.5% per animal, once a day, 5 times a week
Was applied. Application was performed over 4 weeks and hair removal was performed on the last application day of each week. The determination was made on the day following the last day of each week by primary irritation scoring, using erythema and edema as indices. As a result, in all animals, 1
From -4 weeks, no erythema and edema were observed, and it was judged negative.

[0075]

[Examples] Safety test 3) Acute toxicity test The silk protein hydrolyzate according to the present invention was administered to ddy mice (female, 5 mice per group, body weight 28 g) which were fasted for 4 hours before the test in an amount of 2,000 mg / kg. Oral administration, toxic symptoms,
The degree and the like were observed over time. As a result, no abnormality was observed in all the mice for 14 days, and no abnormalities were found in the dissection results. LD50 was determined to be 2,000 mg / kg or more.

[0076]

EXAMPLES Production of various edible compositions Various edible compositions were produced using the physiologically active composition according to the present invention. The formulation examples are shown below, but the invention is not limited thereto.

1) Foods for Health (Tablets) A composition containing the following additives was tabletted into tablets. % By weight Dry powder of silk protein hydrolyzate 10 2. 2. Plant extract powder (dokudami, carrot, perilla) 30 Royal jelly powder 5 4. Collagen powder 5 5. Lactose 25 6. Corn starch 20 7. Hydroxypropyl cellulose 4 8. Magnesium stearate 1

2) Beverages for Health A health drink was prepared according to the following formulation. % By weight Dry powder of silk protein hydrolyzate 4 2. Honey 15 3. Citric acid 0.1 4. dl-malic acid 0.15. Plant extract (chlorella, cinnamon) 20 6. D-sorbitol solution (70%) 10 7. 7. Sodium benzoate 0.05 Appropriate amount of flavor 9. Purified water 100 residue

3) Juice drink A juice drink was prepared according to the following formulation. % By weight Glucose liquid sugar 33 2. Grapefruit juice 65 3. 3. Dry powder of silk protein hydrolyzate 0.5 Appropriate amount of fragrance 5. Sour seasoning appropriate amount

[0080]

EXAMPLES Production of Various Cosmetic Compositions Various cosmetic compositions were produced using the bioactive composition according to the present invention. The formulation examples are shown below, but the invention is not limited thereto.

1) Production Example of Lotion A lotion was produced according to the following formulation. % By weight Sorbitol 2 2.1,3-butylene glycol 2 3. Polyethylene glycol 1000 1 4. Polyoxyethylene oleyl ether (25E.O.) 25 Ethanol 10 6. Dry powder of silk protein hydrolyzate 1 7 Plant extract (Aloe, Hamamelis) 3 8. pH adjuster qs 9. Preservative appropriate amount 10. Purified water 100 residue

2) Production Example of Emulsion Emulsion was produced according to the following formulation. % By weight Squalane 3 2. Vaseline 1 3. Stearyl alcohol 0.3 4. Sorbitan monostearate 1.5 5. Polyoxyethylene (20) sorbitan monooleate 3 6.1,3-butylene glycol 5 7. 7. Dry powder of silk protein hydrolyzate 0.5 Water-soluble collagen 19 9. Preservative appropriate amount 10. Purified water 100 residue

3) Production Example of Cream A cream was produced according to the following formulation. % By weight Squalane 20 2. Beeswax 5 3. Refined jojoba oil 5 4. Glycerin monostearate 2 5. Sorbitan monostearate 2 6 Polyoxyethylene (20) sorbitan monostearate 2 7. Glycerin 5 8. 8. Dry powder of silk protein hydrolyzate 0.3 9. Beef placenta extract 2 10． Preservatives qs. Purified water 100 residue

4) Production Example of Body Soap A body soap was produced according to the following formulation. % By weight Potassium laurate 15 2. Potassium myristate 5 3. Propylene glycol 5 4. Dried powder of silk protein hydrolyzate 3 5. pH adjuster qs 6. Preservative appropriate amount 7. Purified water 100 residue

5) Shampoo A shampoo was manufactured according to the following formulation. % By weight Triethanolamine lauryl sulfate 5 2. 3. Polyoxyethylene lauryl ether sulfate Na 12 3.1,3-butylene glycol 4 Lauric acid diethanolamide 2 5. Disodium edetate 0.1 6. Dried powder of silk protein hydrolyzate 2 7 7. Hydroxypropyl chitosan 0.4 Appropriate amount of fragrance and preservative 9. Purified water 100 residue

6) Rinse A rinse was produced according to the following formulation. % By weight Stearyl trimethyl ammonium chloride 2 2. Cetostearyl alcohol 2 3. Polyoxyethylene lanolin ether 3 4. Propylene glycol 5 5. Dry powder of silk protein hydrolyzate 2 6 Appropriate amount of pH adjuster 7. Preservative appropriate amount 8. Purified water 100 residue

7) Hair Tonic A rinse was produced according to the following formulation. % By weight Ethanol 50 2. Ethyl oleate 1 3. Polyoxyethylene (40) hydrogenated castor oil 24 4. Plant extract (Emophilus radiata, assembly, Hamamelis) 10 5. Dry powder of silk protein hydrolyzate 0.5 Purified water 100 residue

8) Production Example of Bath Agent (Type A) A bath agent was manufactured according to the following formulation. % By weight Sodium bicarbonate 56 2. 2. anhydrous sodium sulfate 30 Borax 2 4. Dry powder of silk protein hydrolyzate 10 5. Extracted plant powder (chamomile, touki) 2

9) Production Example of Bath Agent (Type B) A bath agent was manufactured according to the following formulation. % By weight Refined jojoba oil 5 2. 2. Polyoxyethylene sorbitan monolaurate 20 Glycerin monostearate 5 4. Liquid paraffin 2 5. Lauric acid diethanolamide 3 6. 6. Dry powder of silk protein digest 10 7 Plant extract (Peony, Aloe vera, Chimpi) 10 8. Purified water 100 residue

According to the present invention, a molecular weight of 300 obtained by hydrolyzing a protein silk fibroin obtained from silk can be obtained.
A bioactive composition comprising up to 5,000 peptides was provided. Since the physiologically active composition according to the present invention has an immunostimulating action, an antioxidant action, and an anticarcinogenic promoting action, it is useful for maintaining beauty and health by effectively utilizing it for food, medicine, cosmetics, and the like. Conceivable.

[Brief description of the drawings]

FIG. 1 shows a chart of fractionation of a silk decomposition product by Sephadex G-25.

FIG. 2 shows a molecular weight calibration curve with known molecular weight substances.

FIG. 3 shows the antioxidant action of a silk protein degradation product.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁶ Identification symbol FI // A23L 2/52 A23L 2/00 F

Claims (1)

[Claims] 1. A bioactive composition comprising a peptide having a molecular weight of 300 to 5,000 obtained by hydrolyzing silk protein.