JPH10265397A - Agent for preventing obesity - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain the obesity-preventing agent capable of preventing the obesity caused by the excessive intake of energy by including a mixture comprising the extracts of two kind of specific plants. SOLUTION: This agent for preventing the obesity contains a mixture comprising (A) the extract of Galcinia cambogia containing (-)-hydroxycitric acid and (B) the extract of mulberry leaves containing 1-deoxynojirimycin preferably in a (A):(B) weight ratio of 1:(0.2-5). The obesity-preventing agent is used e.g. for confections such as biscuits and cookies, ice creams, various kinds of juices or drinks, etc. Since the natural materials are used, the agent cause no trouble on acute toxicity.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

[0001] The present invention relates to an obesity suppressant for preventing obesity caused by excessive intake of energy.

[0002]

2. Description of the Related Art Obesity, which has been increasing in recent years, is closely related to daily eating habits. In particular, it has been pointed out that excessive intake of dietary energy and lack of exercise are important factors. Obese people have many adult diseases such as hypertension, diabetes, and arteriosclerosis, and prevention of obesity is an important health measure from the viewpoint of adult disease prevention. Under these circumstances, the prevention of obesity has been recognized as an important issue in terms of improving dietary habits, and it is not necessary to provide foods with a low calorie formula, as well as to suppress the absorption and production of fat. There is a strong demand for the development of substances that suppress the absorption of substances and sugars. Appetite suppressants, digestion and absorption inhibitors, fat accumulation inhibitors,
Although drugs such as metabolic accelerators are used, the use of these drugs in daily food is prohibited by the Food Sanitation Law. It has been demanded. (-)-Hydroxycitric acid is known as an example of a natural product that suppresses the production of fatty acids that cause obesity (ACSullivan, Amer.
J. Clinic Nutrition, 30, 767 (1977)). This substance is a citric acid derivative that inhibits the production of fatty acids by inhibiting ATP citrate lyase, an enzyme that converts citric acid generated from glucose to acetyl-CoA in the fatty acid synthesis pathway in the body. Has been shown to suppress. At this time, the remaining glucose is accumulated as glycogen, so that it gives a feeling of fullness and alleviates the feeling of fatigue. This substance is abundantly contained in the pericarp of a plant from South Asia, Garcinia gambodia, also known as Malabar Tamarind, and its extract is used as a diet material in many foods and beverages. As an example of a natural product that suppresses the absorption of saccharides, a gymnema sylvestre plant, which is native to India, has attracted attention, and various health foods using this have been commercialized. Also, 1-deoxynojirimycin is known as an example of a substance that inhibits the decomposition and absorption of sugar. 1-Deoxynojirimycin is a substance contained in mulberry plants, and 0.1 to 0.2% is contained in dried mulberry leaves. It has been shown that this substance has the effect of delaying the absorption of glucose in the intestinal tract and inhibiting the rise of blood glucose by strongly inhibiting the action of α-glucosidase, a disaccharide-degrading enzyme. (Y.Yoshik
uni, Agric. Biol. Chem., 52, 121 (1988)).

[0003]

It is important to take a low-calorie diet to prevent obesity. However, in the low-calorie diet menu, the content of the food to be taken is limited, and the user may enjoy a rich diet. Can not. On the other hand, if a certain natural product is ingested at the same time, the absorption of sugar and the generation and accumulation of fat can be suppressed, and if it becomes possible to prevent obesity, it is necessary to consider a low-calorie diet Thus, it is possible to provide an effective means for preventing obesity. The aforementioned Garcinia Cambodia peel extract containing (-)-hydroxycitric acid has already been used in many health foods as a diet material, but has a problem to be improved in terms of flavor. That is, since (-)-hydroxycitric acid is a derivative of citric acid, it has a strong sour taste, has a quantitative limit for use in foods, and is also desired to reduce the amount used from the viewpoint of product cost. ing. On the other hand, 1-deoxynojirimycin contained in mulberry plants strongly inhibits α-glucosidase, which degrades disaccharides such as maltose and sugar produced from starch into glucose, and has the effect of suppressing the absorption of glucose from the intestinal tract. However, when this is used for daily food, there is a problem in terms of an effective amount.
That is, 1-deoxynojirimycin in rats
The dose is 6 to 60 mg / kg of rat body weight (Y. Yoshikuni, A
gric. Biol. Chem., 52, 121 (1988), which, when converted to humans, results in an intake of 0.3 to 3 g at a time. However, when using a mulberry leaf extract containing 1-deoxynojirimycin, the intake per serving is 75 to 750, calculated from the 1-deoxynojirimycin content.
g is the effective amount. Such amounts cannot be used in everyday foods.

[0004]

Means for Solving the Problems In order to solve the above-mentioned problems, the present inventors have investigated the enhancement of other substances for suppressing obesity in a pericarp extract of Garcinia cambodia containing (-)-hydroxycitric acid. After studying the effects,
It was found that the combined use of a pericarp extract of Garcinia cambodia containing (-)-hydroxycitric acid and a mulberry leaf extract containing 1-deoxynojirimycin exhibited an obesity-suppressing effect at a lower concentration. Invented the invention. That is, the present invention relates to a pericarp extract of Garcinia cambodia containing (-)-hydroxycitric acid,
An anti-obesity agent comprising a mixture with a mulberry leaf extract containing deoxynojirimycin.

[0005]

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in detail. The component (-)-hydroxycitric acid used in the present invention does not necessarily need to be pure,
Of course, it may be used in the form of an extract of Garcinia cambodia peel containing (-)-hydroxycitric acid. In the present invention, the extract containing 50% of commercially available (-)-hydroxycitric acid (Ortho Corporation, Citrimax) was used. The other 1-deoxynojirimycin used in the present invention is contained in mulberry in plants, and its presence has not been confirmed in plants other than mulberry. In the present invention, 1-deoxynojirimycin need not be purely taken out and used, and a sufficient effect can be obtained by using a mulberry leaf extract containing 1-deoxynojirimycin. In order to obtain a mulberry leaf extract containing 1-deoxynojirimycin, water or an alcohol such as hydrous ethanol is added to the dried mulberry leaf powder and extracted. The extract is centrifuged to remove insolubles, concentrated under reduced pressure, and freeze-dried to obtain a mulberry leaf extract. This extract contains 0.4-1% of 1-deoxynojirimycin.
include.

The above mixture in the agent for preventing obesity of the present invention can be prepared by mixing an extract of (-)-hydroxycitric acid-containing garcinia cambodia peel and a mulberry leaf extract containing 1-deoxynojirimycin. . The mixing ratio at this time is such that 0.2 to 5 of the mulberry leaf extract containing 1-deoxynojirimycin is added to 1 (weight ratio) of the extract of (-)-hydroxycitric acid-containing garcinia cambodia peel. It is effective and has a synergistic effect. In this case, when the weight ratio of the mulberry leaf extract containing 1-deoxynojirimycin is less than 0.2 or more than 5, no usefulness is recognized from the relation between dose and effect.

The obesity-suppressing effect of the mixture prepared as described above can be evaluated using gold thioglucose-treated obese model mice. It is known that gold thioglucose treatment destroys satiety centers in the brain, causing overeating and inducing obesity. The mice were fed with 8 to 0.3% to 1.5% of the diet prepared above.
After free intake for a week, the body weight suppressing effect was measured. As a result, compared with the case of using the (-)-hydroxycitric acid-containing garcinia cambodia peel extract alone, about 1/5 of the mulberry leaf extract containing 1-deoxynojirimycin was used in combination.
It was confirmed that the same amount exhibited the same obesity suppression effect.

[0008] The obesity preventive agent thus obtained is a water-soluble organic polymer compound such as dextrin, CMC,
A powder or granule obtained by spray drying together with an excipient such as gum arabic or gelatin can be suitably used, but may be a tablet or a capsule.
When using this obesity preventive agent, it is effective to take it before or during a meal.

The use of the agent for preventing obesity of the present invention in foods does not particularly designate a food group, and examples thereof include biscuits, cookies, chewing gums, chocolates, candies and the like classified as confectionery. It can also be used with other nutrients such as vitamins. In addition, it is used for ice confectionery such as ice cream and sherbet, cold confectionery, various juice drinks, coffee, soups, healthy drinks and the like. Since the obesity preventive agent of the present invention uses the natural product as described above, there is no problem in acute toxicity at all.

[0010]

EXAMPLES The present invention will be described in more detail with reference to the following Examples, but it should not be construed that the present invention is limited thereto. Preparation Example 1 Five liters of distilled water was added to 1 kg of dry powder of mulberry leaves, and the mixture was stirred and extracted while heating. 5 liters of ethanol was added to this extract and left overnight, and the resulting precipitate was removed by centrifugation. This solution was concentrated under reduced pressure at 45 ° C. or lower and freeze-dried to obtain 150 g of a mulberry leaf extract containing 1-deoxynojirimycin. The 1-deoxynojirimycin content in this extract was measured by a gas chromatography method.
That is, a certain amount of the extract is dissolved in 10 times of distilled water,
The resultant was applied to an ex50X4 (H type) column, sufficiently washed with distilled water to remove an acid neutral portion, and then eluted with 2N ammonia water. The eluate was concentrated under reduced pressure, trimethylsilylated, and analyzed by a gas chromatography device. As a result, the content of 1-deoxynojirimycin in the obtained mulberry leaf extract was 0.4 to 1%.

Preparation Example 2 A mouse basic feed having the composition shown in Table 1 was used for the obesity suppression test.

[0012]

[Table 1]

To the feed having this composition, a Garcinia cambodia peel extract containing 50% of (-)-hydroxycitric acid (Orito Corporation, Citrimax) and the mulberry leaf extract of Preparation Example 1 are shown in Table 2. After mixing well, the mixture was molded into a solid and used as a feed for a mouse obesity suppression test.

[0014]

[Table 2]

Example 1 Five-week-old ICR mice were administered with gold thioglucose (0.6 g / kg body weight) dissolved in physiological saline intraperitoneally, and the body weight was measured for 2 weeks. Successful mice were selected and used for an obesity suppression test. Groups were formed so that the body weight at the start of feed administration was approximately equal in each group, and 6 animals were housed in one cage in all groups, with a total of 12 animals in two cages per group. Feed and drinking water were available ad libitum and bred for 8 weeks. During that time, we performed weekly weight measurement and twice weekly food consumption measurement,
The feed was replaced each time. The measured values were shown as the average value ± standard error. Table 3 shows the results of the weight suppression test.

[0016]

[Table 3]

As can be seen from the results in Table 3, when the Garcinia Cambodia peel extract and the mulberry leaf extract were administered in combination, the concentration was lower at a lower concentration than when the Garcinia Cambodia peel extract was administered alone. A weight-suppressing effect was observed. That is, this combined use exerted an equivalent weight-suppressing effect at 1/5 the amount of Garcinia Cambodia peel extract alone. The mixing ratio of the Garcinia Cambodia peel extract and the mulberry leaf extract was 0.2 to 5 weight per 1 weight of the Garcinia Cambodia peel extract. As can be seen from Table 3, there is no difference in the total food intake between the test groups, and it is clear that the weight suppression effect at that time is not caused by the suppression of the food intake.

 ──────────────────────────────────────────────────続 き Continued on the front page (72) Inventor Shigeo Sudo 6-25-7 Nakano, Nakano-ku, Tokyo (72) Inventor Makoto Hasegawa 5-13-306, Morino, Machida, Tokyo

Claims (2)

[Claims] 1. An anti-obesity agent comprising a mixture of a Garcinia cambodia peel extract containing (-)-hydroxycitric acid and a mulberry leaf extract containing 1-deoxynojirimycin. 2. The mixing ratio of (-)-hydroxycitric acid-containing Garcinia cambodia peel extract and 1-deoxynojirimycin-containing mulberry leaf extract is expressed by weight ratio of (-)-hydroxycitric acid. A mulberry leaf extract containing 1-deoxynojirimycin is 0.2 to 5 with respect to a Garcinia cambodia peel extract 1 containing.
Anti-obesity agent.