JPH07330555A - Hair tonic - Google Patents

Abstract

PURPOSE:To obtain a hair tonic excellent in the proliferation promotive activity for human follicle cells, depilation preventiveness and trichogenous promotive ability, thus useful for treating and prevention of masculine apopecia and diffused apopecia, containing, as a trichogenous/hair tonic promotive ingredient, an solvent extract from paulownia leaves. CONSTITUTION:This hair tonic contains, as an active ingredient, an extract derived from extracting (A) paulownia leaves belonging to a plant: Paulownia tomantosa Steud or an homologous plant therefor (B) a solvent such as (hydrous) methanol, (hydrous) ethanol, (hydrous) acetone, n-hexane or toluene. It is preferable that the amount of the component B to be used be 5-20 (esp. 10-15) wt. times the component A.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a novel hair-growth agent useful for medical purposes, and more specifically, containing an extract of paulownia leaves as an active ingredient to prevent alopecia, hair loss, and hair growth. The present invention relates to a hair restorer applied externally to the scalp for promotion.

[0002]

BACKGROUND OF THE INVENTION Generally, there are many types of alopecia according to their causes and symptoms, but the main ones are male pattern baldness, diffuse alopecia and alopecia areata. . Of these, alopecia areata is transient and is often completely cured by treatment in the dermatological field, but the former two are intractable. Among them, male pattern baldness is
Genetic factors are strong, and the etiological factors include androgen theory, peripheral circulatory disorder theory, and other, although not the main cause, abnormal sebum secretion, excessive dandruff occurrence, malnutrition theory, etc. It is said that sexual alopecia is caused by insufficient blood flow to hair follicles and excessive hair care such as washing and brushing.

Based on these causes, the following ingredients are used in conventional hair restorers. Cemblyx extract, garlic extract, carpronium chloride, ginger tincture, capsicum tincture, vitamin E, pyridoxine and its derivatives to improve abnormal sebum secretion, isopropylphenol, hinokitiol, salicylic acid, anti-inflammatory to improve overproduction of dandruff, which improves peripheral circulation. Glycyrrhizic acid, hinokitiol, diphenhydramine chloride as an agent, Photosensitizer 301, hinokitiol as a cell activator,
Placenta extract, pentadecanoic acid glyceride, etc. have been used.

However, although these ingredients are effective to some extent in improving individual symptoms, they have not yet improved hair loss. In addition, since male hormones are involved in the onset of androgenetic alopecia, female hormones that antagonize male hormones are used, but side effects appear, so the amount sufficient to obtain efficacy is sufficient. It could not be blended, and the expected effect was not improved.

As described above, at present, a decisive method for improving androgenetic alopecia and diffuse alopecia is not known, but alopecia is the growth phase (the period when hair grows) that suppresses the proliferation of hair follicle cells. , 90% or more of the normal human hair is in the growth phase. The hair that should be in the growth phase is in the rest phase (hair growth stops,
At the time of hair loss, 10% of normal human head hair is at this time. Therefore, a substance that promotes the proliferation of hair follicle cells or a substance that converts resting hair into growing hair can be an effective hair-growing agent.

The present invention has an effect of promoting the growth of human hair follicle cells that make up human hair follicles and forming hair, and an effect of promoting hair growth in mice, which is excellent for use in humans to prevent hair loss and promote hair growth. An object of the present invention is to provide a hair restorer having

[0007]

[Means for Solving the Problems] The present inventors have been conducting research for many years in order to develop a hair-growth agent having a hair-preventing / preventing or hair-growing / hair-growth effect. as a result,
A hair-growing agent mainly composed of an extract of paulownia leaves has a human hair follicle cell growth promoting effect and a hair growth promoting effect in mice,
In a clinical test used in humans, it was found to be effective in preventing / preventing hair loss and has an excellent hair growth / hair-growth effect, and the present invention has been completed based on this finding.

That is, the present invention is based on Paulownia (scientific name: Paulowinia
tomantosa Steud. or its homologous plant), and an extract obtained by extracting leaves of an extract with an extraction solvent, as a hair growth and hair growth promoting component.

The paulownia leaf used in the present invention refers to a kiri (scientific name: Paulowinia tomantosa Steu
d.) or its cognate plant leaves. The leaves of paulownia contained eleostearic acid, etc., and were applied to skin diseases, but their effects are not strong and they are not often used. It has not been proved so far that the extract of paulownia leaves has a hair-growing effect in basic and clinical studies.

The hair restorer of the present invention contains an extract obtained by extracting such paulownia leaves with an extraction solvent as a hair growth and hair growth promoting component. Although the component composition of such a paulownia leaf extract is not necessarily clear, it can be obtained by the following method. First, paulownia leaves are used as they are, or are appropriately crushed or shredded as needed.

The paulownia leaves in such a state are extracted with an extraction solvent. The extraction solvent is selected from hot water or hydrous organic solvents. When extracting using hot water, the paulownia leaves are added to hot water in an amount of 5 to 20 times, preferably 10 to 15 times the weight of paulownia leaves for 30 minutes to 4 hours, preferably 1
Extraction may be performed by immersing for ~ 2 hours.

Specific examples of the water-containing organic solvent include water-containing methanol, water-containing ethanol and water-containing acetone. When extraction is performed using such a hydrous organic solvent, an amount of 5 to 20 times the weight of paulownia leaves, preferably 10 to 15 times the weight of the hydrous organic solvent is added and heated under reflux or at room temperature. The extraction may be carried out by allowing it to stand for 3 days or longer, preferably 1 week or longer. The ratio of the organic solvent in the hydrous organic solvent is 50% or less, preferably 30% or less.

The extract of paulownia leaves is obtained by filtering the extract obtained as described above and then concentrating it to remove the solvent.

The hair-growing agent of the present invention contains the paulownia leaf extract thus obtained as a hair-growth and hair-growth promoting ingredient, that is, an active ingredient. The hair-growth and hair-growth promoting component obtained as described above can be dissolved in oils and fats such as hydrous ethanol and camellia oil, cream, etc., and used as a hair-growth agent in the form shown below. The paulownia leaf extract obtained as described above is usually blended as an extract solid content in a proportion of 0.001 to 20% by weight, preferably 0.1 to 10% by weight, based on the entire hair restorer. .

Other raw material components used in the hair restorer of the present invention include hydrocarbons, waxes, oils and fats, esters, higher fatty acids, higher alcohols, and interfaces which are usually applied to hair restorers. Activator, fragrance, pigment, preservative, antioxidant,
UV protectors, alcohols, pH adjusters, and further bioaging inhibitors such as elastin, collagen, hyaluronic acid, vitamins, minerals, and other medicinal ingredients suitable for the purpose of the present invention Can be prepared.

The hair-growing agent of the present invention can be in various forms. There are liquids, creams, ointments, gels, aerosols, etc., whose names include hair creams, hair tonics, hair lotions, hair treatments, hair liquids, hair mousses, hair gels, hair sprays, hair shampoos, hair rinses, etc. .

Hereinafter, the present invention will be described in detail with reference to Examples, but the scope of the present invention is not limited to these Examples.

【Example】

Production Example 1 200 g of paulownia leaves were added to 2 liters of water and subjected to boiling extraction for 1 hour to extract a boiling water-soluble component to obtain 1.7 liters of an extract. Next, the water of the extract was distilled off and the residue was dried to obtain 31.6 g of a black-brown tung leaf extract.

Production Example 2 200 g of paulownia leaves were added to 2 liters of 30% ethanol,
It was extracted at room temperature for 1 week and filtered. The filtrate is concentrated to 30
% Ethanol was distilled off and the residue was dried to obtain 28.0 g of a dark green tung leaf extract.

Example 1 5.0 g of the blackish brown extract obtained in Production Example 1 was added to 40%.
After dissolving in 1 liter of ethanol, the mixture was allowed to stand in a cool place for 3 days and then filtered to obtain a hair restorer. Whether the hair-growth agent (test drug) thus obtained has an effect of promoting mitotic proliferation of human hair follicle cells was confirmed by a human hair follicle cell growth-promoting test using the following extracted hair follicles. did.

[Human hair follicle cell proliferation promoting test using the removed hair follicles] 1/3 below the growing hair follicles removed from the human scalp
Were dispersed by trypsin treatment to obtain a cell suspension. The cells were coated with type IV collagen and had a diameter of 35 m.
Approximately 30,000 cells were placed in a tissue culture dish of m and cultured at 37 ° C. under the conditions of 5% carbon dioxide gas and 95% air. As the culture medium, KGM medium (manufactured by Kurabo) was used. When the hair follicle cells proliferated, they were detached with trypsin to obtain a cell suspension. The cells were coated with type IV collagen and had a diameter of 35.
About 20,000 cells were placed in a tissue culture dish of mm and cultured. Twenty-four hours after the start of culture, the medium was changed to a medium containing 0.001% of a hair growth agent. The medium was replaced with a new medium every 48 hours, and the cells were cultured for 144 hours. Proliferated cells were detached with trypsin and counted with a hemocytometer. Further, the cell number was expressed as a relative value when the control drug (control) was set to 100%. Table 1 shows the number of cells after 144 hours of culture. The control drug (control)
For this, 70% ethanol was used. From Table 1, it was confirmed that the hair-growing agent containing the extract of paulownia leaves had a remarkable effect of promoting human hair follicle cell growth.

[0021]

[Table 1]

Example 2 [Hair regrowth rate test in C3H / HeS1c mice] The following hair regrowth rate test was carried out with reference to the reference of Ogawa et al. That is, C3H / HeS1c mice (7 weeks old)
The back of each animal was shaved, and 5 mice in the resting period of the hair cycle were selected as one group. This mouse was rubbed with three samples of the foliage extract-containing hair restorer prepared in Example 1, a flozin solution, and a 70% ethanol solution once a day (0.1 ml / animal / 8 cm ² / da).
y), the state of hair growth was observed for 4 weeks, the proportion of newly growing hair on the hair cutting portion was calculated, and the recurrence hair rate was calculated. The results are shown in Table 2. From Table 2, it was confirmed that the hair growth agent containing the paulownia leaf extract of the present invention has an extremely excellent effect of promoting hair growth in mice, as compared with the flodin solution and 70% ethanol solution.

[0023]

[Table 2]

Example 3 61 kinds of alopecia patients (35 cases of male pattern baldness, 22 cases of diffuse alopecia, 4 cases of alopecia pityroides) so that the kind and degree of each group were equal. The test was divided into 3 groups, and a clinical test was carried out using the hair growth agent containing the paulownia leaf extract prepared in Example 1, the flozin solution, and the 70% ethanol solution as test agents, and the efficacy was evaluated. Topical application to the head was carried out twice a day for 4 months each day, the hair growth state was judged 4 months later, and the degree of improvement was evaluated in the following 4 stages. The results are shown in Table 3.

[Criteria] Significantly improved: New hair is observed (staple hair). Improvement: New hair is seen (soft hair). Slight improvement: Reduction of hair loss is observed. Invariant: No change is observed.

[0026]

[Table 3]

From the results shown in Table 3, in the hair growing agent containing the paulownia leaf extract of the present invention, an improvement of 50% or more (of which a remarkable improvement is 14
%), Which is 31% of the flozin solution (of which 0 is a marked improvement).
%), 15% of 70% ethanol solution (of which significant improvement is 0
%), And the hair growth effect of the foliage extract-containing hair restorer was clearly demonstrated. During the study period, no side effects suspected to be due to the drug were observed in any of the groups.

[0028]

The hair restorer of the present invention constitutes human hair follicles,
It has the effect of promoting the growth of human hair follicle cells that make up hair and the effect of promoting hair growth in mice, and is excellent in preventing hair loss in humans.
A hair growth promoting effect is obtained. Therefore, the hair restorer of the present invention is
It can be effectively used for treating or preventing alopecia such as androgenetic alopecia and diffuse alopecia.

Claims (2)

[Claims] Claim 1. Paulownia (scientific name Paulowinia tomantosa Steu
d. or its homologous plant), and an extract obtained by extracting leaves with an extraction solvent as a hair growth and hair growth promoting component. 2. The hair restorer according to claim 1, wherein the extraction solvent is selected from hot water and hydrous organic solvents.