JPH07260742A - Electrophoresis device - Google Patents

Abstract

PURPOSE:To easily extract the molecules at desired position in distribution with the instruction by a computer by inputting an electrophoresis pattern in the computer of an electrophoresis device without transferring to a photograph plate and the like. CONSTITUTION:The title device has a movable table provided with an ultraviolet light source, a photoelectric multiplier 12 and a sampler for gel cutout 11, which obtains the electrophoresis pattern of the molecules tagged with fluorescence during or after electrophoresis in electrophoresis tanks 19, 20 by scanning with the ultraviolet light. Simultaneously, such data as electrophoresis pattern obtained by more finely dividing the particular sample obtained by the sampler 11 for cutting out molecules which is locally placed, are displayed on a screen of a display coupled with a computer 1.

Description

Detailed Description of the Invention

[0001]

The present invention relates to a protein or DNA (De
soxyribonucleic acid Abbreviation of desoxyribonucleic acid) Electrophoresis device used for fractionation of fragments, etc., especially detection by scanning on electrophoretic gel with a head using an ultraviolet light source, electron multiplier, optical system and sampler for sampling Then, the detection data is input to a computer, and based on the detection data, a desired gel portion can be cut out by the sampler and a pattern is displayed on a display device connected to the computer.

[0002]

2. Description of the Related Art Generally, when a fraction of a protein or a DNA fragment is fractionated by electrophoresis, a specific portion is taken out and subjected to various treatments (PCR: polymerase chain reaction, etc.). Further, when the electrophoresis is further performed, first, it takes a labor to cut out the specific portion, and the labor is not easy. Further, the electrophoretic pattern was transferred to a photographic dry plate or the like to be read by an expert and analyzed.

Therefore, the electrophoretic device with a water storage tank is 198
November 20, 2013 Kodansha Co., Ltd. "Molecular Biology Experiment Manual" p. 99 to 102, and in this case, an example of an agarose gel is 19 as an example of using the gel.
September 10, 1983, "Principle of gene manipulation" published by Baifukan Co., Ltd. 6-9.

[0004]

In the above-mentioned prior art,
It is not easy because it takes time and labor to cut out a specific portion fractionated by electrophoresis. Further, conventionally, it was difficult to extract molecules distributed at each position only by transferring the electrophoretic pattern onto a photographic plate or the like, and it was particularly difficult to determine the base sequence of DNA.

Therefore, the present invention proposes a novel electrophoretic device which eliminates the above-mentioned drawbacks.

[0006]

The present invention provides an ultraviolet light source,
Having a movable table equipped with a photomultiplier tube and a gel excision sampler, fluorescently labeled molecules are electrophoresed according to scanning by the ultraviolet light source during or after electrophoresis in a electrophoresis tank. Is displayed on the screen of a display device coupled to the computer, and a specific portion of the electrophoretic pattern can be cut out by a sampler controlled by the computer.

[0007]

According to the electrophoretic device of the present invention, it is possible to reduce the time and labor required for the conventional gel cutting, and to obtain the electrophoretic pattern of the further subdivided molecules obtained from the molecules cut out by the sampler as extraction data. , It can be electronically processed, and based on that, it can be displayed on a monitor connected to a computer, facilitating data storage,
Furthermore, the base sequence of DNA and the like can be quickly determined.

[0008]

DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention will be described with reference to the drawings. FIG. 1 is a block diagram showing the configuration of an electrophoretic device of the present invention, FIG. 2 is a state diagram showing an electrophoretic pattern and a specific molecule extraction block, and FIG. FIG. 3 is a perspective view showing the extraction capsule of FIG. In FIG. 1, reference numeral 1 is a computer that generates various data processing and drive signals, and is a monitor 2 as a display device.
Are combined.

3 is an interface (I / F), 4,
Reference numerals 5 and 6 denote an X table, a Y table and a Z table which are moved in the X direction, the Y direction and the Z direction by the X direction drive source 7, the Y direction drive source 8 and the Z direction drive source 9, respectively. Reference numeral 10 is a sampler 11, a photomultiplier tube 12, and a lens 1.
3 head, an optical fiber 15 as a light conducting means from a UV light source (ultraviolet light source) 14 to the lens 13.
UV rays are supplied via. The head 10 rotates about a rotating shaft 16 so that the head 10 is set at a predetermined position by the X table 4.

Reference numeral 17 is a sampler driving device, 18 is a power source for a photomultiplier tube, 19 is an agarose gel electrophoresis tank, 20 is an acrylamide gel electrophoresis tank, and an optical fiber 15
UV rays supplied through the lens irradiate each electrophoresis tank 19 or 20 through the lens 13, the electrophoretic pattern is extracted by the photomultiplier tube 12, and the signal is amplified by the amplifier 21, and the output is A The analog / digital (A / D) conversion by the / D converter 22 and the digital signal obtained as an output are applied to the computer 1 through the interface (I / F) 3 and displayed on the screen of the monitor 2 after data processing. It

Next, the detailed operation of the apparatus will be described. First, when the electrophoresis power source mounted on the Y table 5 is supplied, each molecule is separated according to the electrophoresis speed as shown in FIG. To be painted.

Since each molecule is fluorescently labeled in advance, when the gel is irradiated with an ultraviolet (UV) spot and scanned, the presence of the molecule is detected as the intensity of the visible region. In the head 10 in FIG. 1, a photomultiplier tube 12 is built in as an optical system including an ultraviolet ray as a point light source from an optical fiber 15 and a lens 13 for irradiating the ultraviolet ray on a gel. Detect light.

The head 10 is installed on the X-table 4, and by driving the X-table 4 by an X-direction drive source 7, scanning in the X-axis direction is performed and an intensity image of fluorescence is obtained.

The Y table 5 can be set at an appropriate position by the Y-direction drive source 8 to extract a passage pattern or stop the automatic migration. Further, the head 10 is provided with a sampler mechanism that moves up and down by a sampler driving device 17. For example, a desired molecule is extracted by inserting an extraction capsule as shown in FIG. 3 into a desired position and performing electrophoresis. can do.

In order to cope with the vertical electrophoresis using the acrylamide gel, the head 10 is configured to rotate about the rotation shaft 16 in FIG. 1, and is moved in the Z direction as shown in FIG. 1 if necessary. For this purpose, if the Z table 6 is provided, it is possible to move in each of the X, Y and Z directions, and the electrophoretic pattern can be easily extracted.

The tables (X, Y and Z), the sampler driving device 17, the UV light source 14, and the driving sources 7, 8 and 9 are controlled by a computer 1 provided as a control device through an interface (I / F) 3. To be done.

Further, as described above, the signal obtained by the photomultiplier tube 12 is amplified by the amplifier tube 21 (for example, operational amplifier) and the like, and is converted by the A / D converter 22 into an interface (I / F) after analog / digital conversion. ) 3 is input to the computer 1, processed into data such as patterning, componentization, estimation of molecular weight, etc., and displayed on the screen of the monitor 2 for display.

[0018]

According to the electrophoretic device of the present invention, an ultraviolet light source, an electron multiplier, an optical system can be used without transferring the electrophoretic pattern in the fractionation of proteins or DNA fragments to a photographic plate as in the conventional case. And, a head equipped with a sampler is scanned on an electrophoretic gel to extract a pattern, and a desired gel portion can be cut out based on the data, and as an electrophoretic device, particularly the determination of the base sequence of DNA can be performed quickly. There are advantages such as being possible, and the effect is extremely large.

[Brief description of drawings]

FIG. 1 is a block diagram showing an electrophoretic device of the present invention.

FIG. 2 is a state diagram of an electrophoresis pattern and a specific molecule extraction block in the same apparatus.

FIG. 3 is a perspective view of an extraction capsule for molecular extraction in the same device.

[Explanation of symbols]

 1 Computer 2 Monitor 3 Interface 4 X Table 5 Y Table 6 Z Table 10 Head 11 Sampler 12 Photomultiplier Tube 13 Lens 19, 20 Electrophoresis Tank 22 A / D Converter

Claims (3)

[Claims] 1. An ultraviolet light source, a photomultiplier tube, and a movable table equipped with a gel cutting sampler, wherein the fluorescent-labeled molecules are electrophoresed in or after electrophoresis in an electrophoresis tank. The electrophoretic pattern is detected by the electron multiplier according to the scanning by the light source, an electrophoretic pattern is obtained in the computer, the electrophoretic pattern is displayed on the screen of the display device connected to the computer, and the polar molecules can be extracted by the sampler. Electrophoresis device. 2. A first movable table provided with an ultraviolet light source, a photoelectron amplification tube, and a gel cutting sampler, and a second movable table for moving the electrophoresis tank in a direction different from that of the first movable table. A fluorescently labeled molecule during or after electrophoresis in each of the electrophoresis tanks, detected by the electron multiplier according to scanning by the ultraviolet light source, and an electrophoresis pattern in each of the electrophoresis tanks. An electrophoretic device, characterized in that it is obtained in a computer, is displayed on the screen of a display device coupled to the computer, and is capable of extracting the extremely existing molecules by a sampler. 3. A first movable table provided with an ultraviolet light source, a photomultiplier tube and a gel cutting sampler, and a first movable table for moving the electrophoresis tank in a direction different from the first movable table by 90 °. No. 2 movable table, a third movable table for moving the electrophoretic bath in the first and second movable tables in directions different by 90 ° from each other, and a fluorescent-labeled molecule. During or after the electrophoresis in the migration tank, the electrophoretic pattern is detected by the electron multiplier according to the scanning by the ultraviolet light source to obtain the electrophoretic pattern, which is displayed on the screen of the display device coupled to the computer and is local. An electrophoretic device characterized in that molecules can be extracted by a sampler.