JPH072108B2 - Medium supply molding equipment - Google Patents

Description

Description: TECHNICAL FIELD The present invention relates to a medium supply molding apparatus applied to a titer test of antibiotics in pharmaceuticals or culture of bacteria or clinical isolates existing in nature. .

[Conventional technology]

Conventionally, for the production of a medium used for the above-mentioned titer test or culture, for example, after sterilizing peptone, glucose, yeast extract, meat extract, and agar aqueous solution with high-pressure steam, a fixed amount was dispensed into a Petri dish to solidify the agar. To prepare a base layer agar medium, then, on the base layer agar medium, by mixing the bacteria in the separately prepared agar aqueous solution of the above composition, evenly spread in a thin layer before the agar solidifies. To form the seed layer agar medium. In this case, one worker dripped an agar aqueous solution warmed to a predetermined temperature while controlling the temperature, and another worker uniformly spread the agar into a thin layer before the agar solidified.

[Problems to be solved by the invention]

By the way, in the titer test or culture test, the agar layer with uniform thickness and flatness and levelness can be obtained by spreading the seed layer agar medium into a uniform thin layer. Is important in maintaining the accuracy of. In the above conventional method for producing a medium, it is necessary to have a person who drops an agar aqueous solution while controlling the temperature, and a person who develops into a uniform thin layer before the agar is solidified, and a large amount of a medium with high uniformity. However, there is a problem in that the manufacturing time and the cost are increased when manufacturing.

The present invention is to solve the above problems, it is possible to automatically produce a large amount of medium with high homogeneity,
An object of the present invention is to provide a culture medium supply and molding device capable of reducing the manufacturing time and cost.

[Means for solving problems]

Therefore, the medium supply molding apparatus of the present invention, a medium supply means for dropping a certain amount of the medium in the center of the Petri dish, and a Petri dish rotating means for rotating the Petri dish to develop the medium into a uniform thin layer by centrifugal force. And a petri dish transporting means for automatically transporting the petry dish.

[Action]

In the present invention, for example, as shown in FIG. 1, first, the Petri dish 7 on which the agar medium for the base layer is formed in advance is placed in the case 2
It is placed on the rotating disk 9 through the insertion hole on the front surface, the roller pump 5 is driven, and a fixed amount of the medium is dropped from the pipette 6 to the center of the Petri dish 7, and then the rotating disk motor 8 is used.
When the rotating disc 9 is rotated at a predetermined speed for a predetermined time by
The agar medium containing the bacteria will be uniformly spread on the surface of the base layer agar medium by centrifugal force.

〔Example〕

Embodiments of the present invention will be described below with reference to the drawings. First
FIG. 1 is a perspective view showing an embodiment of the culture medium supply molding apparatus of the present invention, and FIG. 2 is a view for explaining the structure and operation of the roller pump in FIG. In FIG. 1, 1 is a culture medium supply molding device, A is a culture medium supply means, B is a petri dish rotating means, C is a petri dish conveying means, 2 is a case, 3 is a roller pump motor, 4 is a roller pump gear, and 5 is a roller pump gear. Roller pump, 6 pipette, 7 Petri dish, 8 rotating disk motor, 9 rotating disk, 10 fixed block, 11
Is a slider shaft, 12 is a slider drive block, 13 is an extrusion guide, 14 is a slider drive shaft, 15 is a slider drive motor, 16 is a slider drive gear, 17 is a slider cover, 18 is a petri dish receiving rack, and 19 is a petri dish discharge. Hole, 20
Is a power switch, 21 is a temperature controller, 22 is a temperature controller adjusting dial, 23, 24 and 25 are rotational speed adjusting dials. In FIG. 2, 30 is a pump head, 31 is a tube, 32
Is a rotor and 33 is a roller.

In FIG. 1, a culture medium supply / molding apparatus 1 of the present invention comprises a case 2, a culture medium supply means A, a Petri dish rotating means B, and
It is composed of a Petri dish conveying means C. The medium supply means A comprises a roller pump 5 driven by a roller pump motor 3 and a roller pump gear 4, and a certain amount of medium is dropped from the pipette 6 to the center of the Petri dish 7 by the roller pump 5. The petri dish rotating means B is composed of a rotary disc motor 8 provided at the bottom of the case 2 and a rotary disc 9 rotated by the motor 8. The petri dish 7 is mounted on the rotary disc 9. Placed.

On the other hand, the petri dish transporting means C is provided with slider shafts 11, 11 between two opposing fixed blocks 10 and is provided with a slider drive block 12 slidable in the horizontal direction along the slider shaft 11. , Extrusion guide at the tip
A slider drive shaft 14 having 13 passes through the fixed block 10 and is fixed to the slider drive block 12, and
The drive block 12 is connected to a slider drive gear 16 driven by a slider drive motor 15, the rotational force of the slider drive motor 15 is decelerated by the slider drive gear 16, and the slider drive block 12 slides in the horizontal direction. Is configured. Each of the above components is covered with a slider cover 17.

Further, a petri dish receiving shelf 18 and a petry dish discharge hole 19 are formed on the side surface of the case 2, a petri dish insertion hole (not shown) is formed on the front surface of the case 2, and a lower portion of the front surface of the case 2 is formed. Is a power switch 20, a temperature controller 21, an adjustment dial 22 for the temperature controller, the motors 3, 8, 15 described above.
Adjustment dials 23, 24, 25 for the rotational speed of are provided.

Next, the structure and operation of the roller pump 5 will be described with reference to FIG. In the roller pump 5, a tube 31 for feeding a culture medium is arranged on the inner wall surface of a pump head 30, and three rollers 33 driven by rotation of a rotor 32 are provided.
Thus, the tube 31 can be fastened to the inner wall surface of the pump head 30. When the rotor 32 is rotated and the roller 33 is pressing the tube 31 against the inner wall surface of the pump head 30 at the position shown in FIG. 2 (a), the rotor 32 is rotated as shown in FIG. 2 (b). One roller 33 rotates while pressing the tube 31, and a fixed amount of liquid stays between the two rollers 33. Further, when the rotor 32 is rotated as shown in FIG.
It rotates while pressing against the inner wall and sends out a fixed amount of liquid to the outlet side.

A method for producing a culture medium using the culture medium supply / forming apparatus of the present invention having the above-described configuration will be described. First, the Petri dish 7 on which the agar medium for the base layer has been formed in advance is placed on the rotating disk 9 through the insertion hole (not shown) on the front surface of the case 2, and the roller pump 5 is driven to set a fixed amount of medium from the pipette 6. Is dropped on the center of the Petri dish 7, and then the rotating disk 9 is rotated by the rotating disk motor 8 at a predetermined speed for a predetermined time. The number of rotations of the motor 8 is set by the viscosity of the agar medium which changes depending on the temperature of the medium and the composition of the culture medium and the type of bacteria used.

As a result, the agar medium containing the bacteria is uniformly spread in a thin layer on the surface of the base layer agar medium by centrifugal force. At this time, the agar medium containing the bacteria is kept at a predetermined temperature (for example,
It is heated to 42 ℃ to 60 ℃), but it can be spread in a uniform thin layer on the surface of the agar medium for the base layer before the agar solidifies.

When this expanding step is completed, the slider drive motor 15 is rotated, the rotational force is reduced by the slider drive gear 16, the slider drive block 12 is slid, and the petri dish extrusion guide 13 is slid in the direction of the petri dish discharge hole 19. By moving, the Petri dish 7 is conveyed to the Petri dish receiving rack 18. After that, the slider drive motor 15 is rotated in the reverse direction, the Petri dish extrusion guide 13 is returned to the original position, and the above steps are repeated to obtain a large number of Petri dishes in which the seed layer agar medium is spread in a uniform thin layer. And it can be manufactured automatically.

The present invention is not limited to the above embodiment, and various modifications can be made.

For example, an automatic feeding mechanism may be connected to the medium supply and molding device of the present invention so that the petri dish 7 is automatically fed and the petri dish receiving rack 18 is automatically ejected.

In addition, in the above-mentioned examples, a mixture of bacteria is formed as a medium, but it may be applied to culture of bacteria existing in nature or culture of clinical isolates.

Further, in the above example, although it is used for the production of the seed layer medium, it may be used for the production of the base layer medium, or it is used for both the base layer medium and the seed layer medium for automation. Further improvement is possible.

〔The invention's effect〕

As described above, in the present invention, a medium supply means for dropping a fixed amount of medium onto a petri dish, a petri dish rotating means for rotating the petri dish and developing the medium into a uniform thin layer by centrifugal force, and a petri dish. By including a Petri dish transporting means for automatically transporting, it is possible to eliminate the need for conventional manual work, and it is possible to automatically produce a large amount of thin-layered medium with high homogeneity. The cost can be reduced.

Moreover, a medium with a high degree of homogeneity can be produced even with a small amount of sample, and in particular, it can be applied to the culture of bacteria existing in nature.

[Brief description of drawings]

FIG. 1 is a perspective view showing one embodiment of the culture medium supply molding apparatus of the present invention, and FIG. 2 is a view for explaining the structure and operation of the roller pump in FIG. DESCRIPTION OF SYMBOLS 1 ... Medium supply molding apparatus, A ... Medium supply means, B ... Petri dish rotating means, C ... Petri dish conveying means, 2 ... Case,
3 ... Roller pump motor, 4 ... Roller pump gear, 5 ... Roller pump, 6 ... Pipette, 7 ... Petri dish, 8 ... Rotating disc motor, 9 ... Rotating disc, 10 ... Fixed block, 11 ... Slider shaft, 12 ... Slider drive block, 13 ... Extrusion guide, 14 ... Slider drive shaft, 15 ... Slider drive motor, 16 ... Slider drive gear, 17 ... Slider cover, 18 ... Petry dish rack, 19 ... Petry dish discharge hole, 20 ... Power switch, 21 ... Temperature regulator, 22 ... Temperature regulator adjustment dial, 23,24,25 ... Rotation speed adjustment dial, 30 ... Pump head, 31 ... Tube, 32 ... Rotor, 33 ... Roller.

Claims (1)

[Claims] 1. A medium supply means for dropping a fixed amount of medium onto the center of a petri dish, a petri dish rotating means for rotating the petri dish to develop the medium into a uniform thin layer by centrifugal force, and a petri dish automatically And a petri dish conveying means for conveying the medium to the medium.