JPH0674935A - How to store acrylamide gel - Google Patents
How to store acrylamide gelInfo
- Publication number
- JPH0674935A JPH0674935A JP4229033A JP22903392A JPH0674935A JP H0674935 A JPH0674935 A JP H0674935A JP 4229033 A JP4229033 A JP 4229033A JP 22903392 A JP22903392 A JP 22903392A JP H0674935 A JPH0674935 A JP H0674935A
- Authority
- JP
- Japan
- Prior art keywords
- gel
- acrylamide gel
- electrophoresis
- support
- acrylamide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 title claims abstract description 30
- 238000000034 method Methods 0.000 claims abstract description 17
- 239000011521 glass Substances 0.000 claims abstract description 6
- 229910052751 metal Inorganic materials 0.000 claims abstract description 6
- 239000002184 metal Substances 0.000 claims abstract description 6
- 229920003023 plastic Polymers 0.000 claims abstract description 6
- 239000004033 plastic Substances 0.000 claims abstract description 6
- 230000005684 electric field Effects 0.000 claims abstract description 4
- 238000001962 electrophoresis Methods 0.000 abstract description 18
- 238000004043 dyeing Methods 0.000 abstract description 2
- 239000000499 gel Substances 0.000 description 44
- 108090000623 proteins and genes Proteins 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 5
- 229920000178 Acrylic resin Polymers 0.000 description 3
- 239000004925 Acrylic resin Substances 0.000 description 3
- 229920001222 biopolymer Polymers 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 239000011888 foil Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 229920000298 Cellophane Polymers 0.000 description 1
- 108010035563 Chloramphenicol O-acetyltransferase Proteins 0.000 description 1
- 241001131785 Escherichia coli HB101 Species 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229920002301 cellulose acetate Polymers 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 108010025899 gelatin film Proteins 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000001155 isoelectric focusing Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000001426 native polyacrylamide gel electrophoresis Methods 0.000 description 1
- 239000000123 paper Substances 0.000 description 1
- 229920003217 poly(methylsilsesquioxane) Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000007447 staining method Methods 0.000 description 1
Landscapes
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Abstract
(57)【要約】
【目的】 電気泳動において支持体として使用するアク
リルアミドゲルに関し、信頼性の高い保存方法を提供す
ることを目的とする。
【構成】 アクリルアミドゲルを支持体として使用する
電気泳動装置において、直流電界を印加して検体の電気
泳動を行なった後、色素を用いて染色し、濃度差をつけ
た該検体を保存する方法として、ガラス板またはプラス
チック板の上に貼着してある前記アクリルアミドゲルの
上に下ろし金状の微細な突起を有するゲルカバーを当接
しておくことを特徴としてアクリルアミドゲルの保存方
法を構成する。
(57) [Summary] [Objective] The object of the present invention is to provide a highly reliable storage method for an acrylamide gel used as a support in electrophoresis. [Structure] In an electrophoresis apparatus using acrylamide gel as a support, a method of storing a sample with a difference in concentration after dyeing with a dye after electrophoresis of the sample by applying a DC electric field A method for storing acrylamide gel is characterized in that a gel cover having fine projections in the shape of a metal plate is brought into contact with the acrylamide gel attached on a glass plate or a plastic plate.
Description
【０００１】[0001]
【産業上の利用分野】本発明は電気泳動装置において電
気泳動の支持体として使用するアクリルアミドゲルの保
存方法に関する。FIELD OF THE INVENTION The present invention relates to a method for storing an acrylamide gel used as a support for electrophoresis in an electrophoresis apparatus.
【０００２】液体中に存在する蛋白質などの分子に直流
電圧を加えると、分子の性質や形状に応じて＋または−
の電荷をもち、＋の電荷を有する分子は陰極の方向に、
また−の電荷を有する分子は陽極の方向に移動する現象
がある。When a direct current voltage is applied to a molecule such as a protein existing in a liquid, + or-depending on the nature or shape of the molecule.
Molecules having a positive charge and a positive charge are directed toward the cathode,
In addition, there is a phenomenon that molecules having a negative charge move toward the anode.
【０００３】この現象を電気泳動と言うが、こゝで、分
子の荷電の状態は電場のpHに関係があり、例えば蛋白質
は酸性環境に存在する場合は＋の電荷をもち、陰極に向
かって、また、アルカリ性環境に存在する場合は−の電
荷をもち、陽極に向かって移動する。This phenomenon is called electrophoresis, and here, the charged state of the molecule is related to the pH of the electric field. For example, when a protein exists in an acidic environment, it has a positive charge and goes toward the cathode. Also, when it exists in an alkaline environment, it has a negative charge and moves toward the anode.
【０００４】かゝる電気泳動は蛋白質の分離を初めとし
て分子量の測定や塩基配列の決定などを行なう上で不可
欠な技術であり、生体高分子を扱う研究に広く用いられ
ている。Such electrophoresis is an indispensable technique for measuring the molecular weight, determining the base sequence, etc., including the separation of proteins, and is widely used in the research dealing with biopolymers.
【０００５】こゝで、生体高分子を含む溶液を保持し、
これに直流電界を加えるのに使用する支持体としてはア
クリルアミドゲルやセルローズアセテートなどが使用さ
れているが、泳動により分離する領域（分画）が明確，
鋭敏であって、且つ分画値の再現性が優れていることが
必要であり、このためアクリルアミドゲルが多く用いら
れている。Here, holding the solution containing the biopolymer,
Acrylamide gel, cellulose acetate, etc. are used as a support to apply a DC electric field to this, but the region (fraction) separated by electrophoresis is clear,
It is necessary to be sensitive and have excellent reproducibility of fractional values, and therefore acrylamide gel is often used.
【０００６】[0006]
【従来の技術】蛋白質を電気泳動により分析する方法に
はSDS-PAGE法( ドデシル硫酸ナトリウム−ポリアクリル
アミドゲル電気泳動法）やNative-PAGE法や等電点電気
泳動法などがある。2. Description of the Related Art Methods for analyzing proteins by electrophoresis include SDS-PAGE method (sodium dodecyl sulfate-polyacrylamide gel electrophoresis method), Native-PAGE method and isoelectric focusing method.
【０００７】そして、従来は、支持体として寸法が約12
0 ×120 ×１ mm 程度のアクリルアミドゲルが使用され
ており、電気泳動処理が終わって後、この結果を保存す
るために、膜状をしたアクリルアミドゲルをガラスまた
はアクリル樹脂よりなるゲル板から剥がし取った後、濾
紙とセロファン紙の間に挟み、真空吸引しながら乾燥す
る方法が採られていた。Conventionally, the size of the support is about 12
An acrylamide gel of about 0 × 120 × 1 mm is used, and after the electrophoretic treatment is completed, in order to save the result, the film-shaped acrylamide gel is peeled off from the gel plate made of glass or acrylic resin. Then, it was sandwiched between filter paper and cellophane paper, and dried by vacuum suction.
【０００８】然し、蛋白質を分離する技術が向上し、ま
た、高感度染色法の発達により支持体の寸法が小さくて
も分析が可能になったことから、現在では約40×50×0.
3mm程度と小形化した支持体が用いられるようになっ
た。However, the technique for separating proteins has improved, and the development of high-sensitivity staining method has made it possible to perform analysis even when the size of the support is small. Therefore, at present, about 40 × 50 × 0.
Supports as small as 3 mm have come to be used.
【０００９】図２はかゝる小形化した支持体の形状を示
すもので、アクリル樹脂よりなるゲル板１の上にアクリ
ルアミドゲルよりなる支持体２があり、この上に染色に
より電気泳動パターン３が形成されている状態を示して
いる。FIG. 2 shows the shape of such a miniaturized support. A gel plate 1 made of acrylic resin has a support 2 made of acrylamide gel on which an electrophoretic pattern 3 is formed by dyeing. Shows the state in which is formed.
【００１０】なお、上部の突出物は把手４である。こゝ
で、アクリルアミドゲルよりなる支持体２の厚さは0.3m
m 程度と薄いためにゲル板１から分離することは困難で
ある。The upper protrusion is the handle 4. The thickness of the support 2 made of acrylamide gel is 0.3 m.
Since it is as thin as about m, it is difficult to separate it from the gel plate 1.
【００１１】そのため、ゲル板１に付いたまゝで乾燥
し、保存していた。然し、乾燥が不充分の場合はアクリ
ルアミドゲルに他のゲルや紙などが付着して取れなくな
る。For this reason, the gel plate 1 was dried until it was stored. However, if the drying is insufficient, other gels, paper, etc. will adhere to the acrylamide gel and it will not be able to be removed.
【００１２】また、湿気に曝すとベタつき、他のゲルや
紙などが付着して取れなくなると云う問題があり保存が
困難であった。Further, there is a problem that it becomes sticky when exposed to moisture, and other gels, papers, etc. adhere to it, making it difficult to store.
【００１３】[0013]
【発明が解決しようとする課題】アクリルアミドゲルは
分解能が優れているために電気泳動装置において高分子
検体などの電気泳動を行なう支持体として使用されてい
る。Acrylamide gel is used as a support for electrophoresis of polymer analytes in an electrophoresis apparatus because of its excellent resolution.
【００１４】然し、支持体として使用されるアクリルア
ミドゲルの厚さは0.3mm 程度と薄く、ゲル板からの剥離
は困難なため、ゲル板に付着したまゝで保存している
が、アクリルアミドゲルは吸湿性が強く、完全に乾燥さ
せることは容易ではなく、吸湿するとベトつくために取
扱いが難しい。However, since the acrylamide gel used as a support has a thin thickness of about 0.3 mm and is difficult to peel off from the gel plate, it is stored as it is attached to the gel plate. It has a strong hygroscopic property, it is not easy to completely dry it, and it becomes sticky when it absorbs moisture, making it difficult to handle.
【００１５】そこで、従来は写真撮影を行なった後、試
験試料を捨てるなどの方法が採られていた。Therefore, conventionally, a method of taking a photograph and then discarding the test sample has been adopted.
【００１６】[0016]
【課題を解決するための手段】上記の課題はガラス板ま
たはプラスチック板よりなるゲル板の上に支持体として
貼着してあるアクリルアミドゲルの上に下ろし金状の微
細な突起を有するゲルカバーを当接しておくことを特徴
としてアクリルアミドゲルの保存方法を構成することに
より解決することができる。[Means for Solving the Problems] The above-mentioned problems are solved by contacting a gel cover having fine protrusions in the form of a metal on an acrylamide gel attached as a support on a gel plate made of a glass plate or a plastic plate. This can be solved by configuring a method for storing an acrylamide gel, which is characterized in that
【００１７】[0017]
【作用】アクリルアミドゲルのように吸湿し易い材料は
紙や他のゲルが付着し易く、一度び付着すると取れなく
なる。[Function] A material such as acrylamide gel, which easily absorbs moisture, tends to adhere to paper or other gel, and once attached, cannot be removed.
【００１８】また、完全に乾燥するにはデシケータなど
に入れて保存すればよいが、取扱いが煩雑である。そこ
で、本発明は表面に下ろし金状の微細な突起を有するゲ
ルカバーを当接しておくことにより、アクリルアミドゲ
ルに他のアクリルアミドゲルや紙や塵埃が付着するのを
防ぐものである。Further, in order to completely dry it, it may be stored in a desiccator or the like, but the handling is complicated. In view of this, the present invention prevents the adhesion of other acrylamide gel, paper, or dust to the acrylamide gel by bringing the gel cover having the fine projections in the shape of a metal down into contact with the surface.
【００１９】図１はゲルカバー５の一例を示すもので、
アクリルアミドゲルとの接触を最小とするために多くの
突起６が配列している。こゝで、突起６の構成材として
はは金属でもプラスチックでもセラミックでもよいが、
取扱いを容易にするためにはなるべく薄く且つ軽いとよ
い。FIG. 1 shows an example of the gel cover 5.
Many protrusions 6 are arranged to minimize contact with the acrylamide gel. The constituent material of the protrusion 6 may be metal, plastic or ceramic.
It should be as thin and light as possible to facilitate handling.
【００２０】このようなゲルカバーを用いると再検査す
る場合に簡単にとることができ、また接触面積が少ない
ために電気泳動の結果が乱されていることもない。ま
た、試料を積み重ねて保存することも可能となる。When such a gel cover is used, it can be easily taken for re-inspection, and since the contact area is small, the result of electrophoresis is not disturbed. It is also possible to stack and store the samples.
【００２１】[0021]
実施例１：（請求項２に対応） 厚さが150 μm のアルミ(Al)箔をプレス成形して図１の
形状をしたゲルカバーを作った。Example 1 (corresponding to claim 2) An aluminum (Al) foil having a thickness of 150 μm was press-molded to make a gel cover having the shape shown in FIG.
【００２２】次に、検体としては大腸菌HB101(pYEJ001)
の粗抽出液から部分精製したクロラムフェニコールアセ
チルトランスファーゼ(Chloramphenicol acetyltransfe
rase略称CAT)を電気泳動装置（名称Phast System, ファ
ルマシア製）を用いてアクリルアミドゲル（品名Phastg
el 10-15％, ファルマシア製) よりなる支持体の上で電
気泳動し、その後、染料( 品名Phastgel Blue R,ファル
マシア製) を用いて染色した。Next, E. coli HB101 (pYEJ001) was used as a sample.
Chloramphenicol acetyltransferase
Rase abbreviated CAT using an electrophoresis device (named Phast System, manufactured by Pharmacia) and acrylamide gel (named Phastg
Electrophoresis was carried out on a support consisting of el 10-15% (Pharmacia), followed by staining with a dye (Phastgel Blue R, Pharmacia).
【００２３】そして、ガラスのゲル板と共に３日間室温
で乾燥させた後、Al箔製のゲルカバーを当接して保存し
た。次に、一週間経過して後、ゲルカバーを外して観察
したがアクリルアミドゲル膜に損傷はなく、また汚染も
なかった。 実施例２：（請求項３に対応） アクリル樹脂を成形して図１の形状をしたゲルカバーを
作った。After drying the glass gel plate at room temperature for 3 days, the gel cover made of Al foil was contacted and stored. Next, after one week, the gel cover was removed and observed, but the acrylamide gel membrane was not damaged and was not contaminated. Example 2: (corresponding to claim 3) An acrylic resin was molded to prepare a gel cover having the shape of FIG.
【００２４】そして、実施例１と同じ検体を用いて電気
泳動を行い、同じ染料を用いて染色した。そして、ガラ
スのゲル板と共に３日間室温で乾燥させた後、プラスチ
ックスよりなるゲルカバーを当接して保存した。Then, the same sample as in Example 1 was used for electrophoresis, and the same dye was used for staining. Then, it was dried at room temperature for 3 days together with a glass gel plate, and then stored by contacting it with a gel cover made of plastics.
【００２５】次に、一週間経過して後、ゲルカバーを外
して観察したがアクリルアミドゲル膜に損傷はなく、ま
た汚染もなかった。Next, after one week, the gel cover was removed and observed, but the acrylamide gel film was not damaged and was not contaminated.
【００２６】[0026]
【発明の効果】本発明の実施により電気泳動処理を行な
ったアクリルアミドゲルよりなる支持体に汚染や破損を
生ずることなく保存することが可能となり、これにより
生体高分子の分析効率を向上することができる。EFFECTS OF THE INVENTION By carrying out the present invention, it is possible to store a support made of acrylamide gel which has been subjected to electrophoresis treatment without causing contamination or damage, thereby improving the analysis efficiency of biopolymers. it can.
【図１】ゲルカバーの一例を示す斜視図である。FIG. 1 is a perspective view showing an example of a gel cover.
【図２】電気泳動に使用する支持体の構成図である。FIG. 2 is a configuration diagram of a support used for electrophoresis.
１ ゲル板 ２ 支持体 ３ 電気泳動パターン ５ ゲルカバー ６ 突起 1 gel plate 2 support 3 electrophoresis pattern 5 gel cover 6 protrusions
Claims (3)
する電気泳動装置において、直流電界を印加して検体の
電気泳動を行なった後、色素を用いて染色し、濃度差を
つけた該検体を保存する方法として、ガラス板またはプ
ラスチック板よりなるゲル板の上に貼着してある前記ア
クリルアミドゲルの上に下ろし金状の微細な突起を有す
るゲルカバーを当接しておくことを特徴とするアクリル
アミドゲルの保存方法。1. An electrophoretic apparatus using acrylamide gel as a support, a sample is electrophoresed by applying a DC electric field, and then stained with a dye to store the sample with a different concentration. As a method, a method for preserving acrylamide gel, characterized in that a gel cover having fine protrusions in the form of a down metal is brought into contact with the acrylamide gel attached on a gel plate made of a glass plate or a plastic plate. ..
徴とする請求項１記載のアクリルアミドゲルの保存方
法。2. The method for storing acrylamide gel according to claim 1, wherein the gel cover is made of metal.
ることを特徴とする請求項１記載のアクリルアミドゲル
の保存方法。3. The method for preserving acrylamide gel according to claim 1, wherein the gel cover is made of plastics.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4229033A JPH0674935A (en) | 1992-08-28 | 1992-08-28 | How to store acrylamide gel |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4229033A JPH0674935A (en) | 1992-08-28 | 1992-08-28 | How to store acrylamide gel |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH0674935A true JPH0674935A (en) | 1994-03-18 |
Family
ID=16885696
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP4229033A Withdrawn JPH0674935A (en) | 1992-08-28 | 1992-08-28 | How to store acrylamide gel |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0674935A (en) |
-
1992
- 1992-08-28 JP JP4229033A patent/JPH0674935A/en not_active Withdrawn
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A300 | Withdrawal of application because of no request for examination |
Free format text: JAPANESE INTERMEDIATE CODE: A300 Effective date: 19991102 |