JPH06284872A - Health and beauty promoter, blood pressure suppressor and constipation improver having sod action - Google Patents

Abstract

PURPOSE:To obtain a healthy and beauty promoter, etc., having safe and excellent SOD action (action to effectively remove O2-considered as toxic to the living body and action on prevention, etc., of diseases) by using rice brans and soybeans as a raw material. CONSTITUTION:This healthy and beauty promoter having SOD action is characterized by inoculating Bacillus subtilis into a culture medium containing rice brans and soybeans, culturing the Bacillus subtilis, filtering the resultant culture, producing a fermentation liquor and blending a concentrate (e.g. 40-50wt.% concentration) of the resultant fermentation liquor with a green tea extract essence (e.g. 40-50wt.% concentration) in an amount of 1-50 pts.wt. expressed in terms of an evaporation residue based on 100 pts.wt. evaporation residue of the fermentation liquor. A flavonoid can be contained in the fermented liquor and the green tea extract essence can contain phytic acid or its salt. The evaporation residue (solid substance) can be used in place of the respective solutions. This promoter can be utilized as a blood pressure suppressor and a constipation improver.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a health food having an SOD action. The present invention provides various diseases caused by active oxygen (O ₂ ⁻ ), for example, diseases caused by blood flow disorders (myocardial infarction, stroke, high blood pressure, menstrual pain, stiff shoulder, neuralgia, back pain, hangover, etc.), adult diseases, It is widely used for the treatment and improvement of internal diseases (cancer, nephritis, hepatitis, diabetes, etc.), beauty and skin diseases (stains, freckles, rough skin, colds, constipation, wrinkles, atopic dermatitis, etc.).

[0002]

2. Description of the Related Art Superoxide dismutase (SOD
Say. ) Is a superoxide radical (superoxide radical) generated by one-electron reduction of an oxygen molecule.
cal, O ₂ ^- disproportionation) (lower formula) catalyzed at a rate close to diffusion control, O ₂ in the cell ^- an enzyme to reduce the concentration. O ₂ ⁻ + O ₂ ⁻ + 2H ⁺ → H ₂ O ₂ + O _{2 If} O ₂ ⁻ is harmful to the living body in recent years, this SOD, which is a scavenging enzyme, should exist to protect the living body from active oxygen toxicity. Therefore, the reaction mechanism, physiological mechanism, and the like are being studied from the viewpoint of being useful for diseases and the like that are considered to be caused by this active oxygen [[reactive oxygen-production / elimination in living organisms Molecular mechanism of action "(2 new editions, published by Kyoritsu Shuppan Co., Ltd., edited by Minoru Nakano) P. Two
23-230)]. In addition, there is a fact that SOD activity is low in cancer cells, and further, although a direct causal relationship between SOD and carcinogenesis is not clear, it is reported that injection of SOD or SOD-like substances into cancer cells suppresses proliferation (description). (P.64).

[0003]

If there is a food that is safe and has an SOD action, it will be extremely useful for human health and beauty, and there is a great need for it. In particular, in a situation where stress is high and many diseases are occurring as in recent years, the necessity thereof is great.

The present invention uses rice bran and soybeans as raw materials and is a health food which is safe and has an excellent SOD action.
Further, it is an object of the present invention to provide a health food having an SOD action, which contains at least one of phytic acid and its salt and a green tea extract.

[0005]

From the above viewpoints, the present inventors diligently studied variously as to whether or not a health food having this SOD activity uses a safe fermented product. The present invention has been completed by discovering that the purpose can be achieved by adding a predetermined additive to a fermentation liquor obtained by fermentation using the above raw materials and filtration. A health food having an SOD action of the first invention is obtained by inoculating Bacillus subtilis in a medium containing rice bran and soybean,
The fermentation liquor produced by culturing and filtering, or the evaporation residue of the fermentation liquor contains the green tea extract or the evaporation residue of the green tea extract.

The "green tea extract" is obtained by extracting tea leaves using methanol, ethanol, ethyl acetate and the like (particularly methanol and ethanol) as a solvent, and the concentration of the extracted components is about 5 to 50% by weight. be able to. And, as shown in the second invention, the mixing ratio of the green tea extract can be 1 to 50 parts by weight in terms of the evaporation residue, based on 100 parts by weight of the evaporation residue of the fermentation liquid. . This is because if it is less than 1 part by weight, the effect cannot be expected sufficiently, and if it exceeds 50 parts by weight, it becomes difficult to taste and drink.

The health food having an SOD action according to the third aspect of the present invention is a fermentation liquor produced by inoculating a culture medium containing rice bran and soybeans with Bacillus subtilis, culturing and filtering, or an evaporation residue of the fermentation liquor. , Flavonoids are contained. The "flavonoid" is widely distributed in the plant kingdom, the two phenyl groups are bonded across the three carbon atoms of the pyran ring, or close to it the structure, so-called C ₆ -C ₃ -C ₆ It is a group of compounds consisting of carbon skeleton. This flavonoid is contained in 13 to 14% by weight based on the evaporation residue in the green tea extract. And the blending ratio of this flavonoid is 0.1 to 100 parts by weight of the fermentation broth (for example, when the extract concentration is 40 to 50% by weight).
It can be about 7 parts by weight. This is because if the amount is less than 0.1 part by weight, the effect cannot be expected sufficiently, and if the amount exceeds 7 parts by weight, it becomes difficult to drink.

The above-mentioned "rice bran" means rice germ, defatted rice germ, rice bran, defatted rice bran and the like, and "soybeans" means defatted soybean, quina flour, soybean flour, soybean dregs and hydrolysates thereof. Etc. Usually, these are added in an amount of 10 to 20 parts by weight of soybeans when the rice bran is 100 parts by weight. The fermentation conditions are usually pH of 7.5 to 10 (especially around 9) and culture temperature of about 40 to 45 ° C. In addition, protease can be used as a medium raw material. This case is useful because it further decomposes the soybean peptide.

The "fermentation solution" may be a solution obtained by culturing the cultured culture fermentation solution as it is, a solution that has been subjected to post-treatment such as decolorization, or a concentrated solution obtained by concentrating the solution. As described above, the evaporation residue (solid content) of this fermentation liquid may be used. The health food having an SOD action of the fourth aspect of the present invention is characterized by containing at least one of phytic acid and a salt thereof, and a green tea extract (or an evaporation residue of the green tea extract).

The above-mentioned "phytic acid" may be synthetic phytic acid or phytic acid extracted and isolated. Also,
As the “salt”, a non-toxic salt is used, and a salt with a metal, an organic base, a basic amino acid, an organic ester residue, etc. is used, and examples thereof include potassium, sodium, arginine,
It may be a salt of ornithine, lysine, histidine, glucamine, monoethanolamine or the like. Since this phytic acid exhibits acidity, a salt thereof having a pH of about 6 to 8 is usually used. It does not matter whether this salt is water-soluble or not,
Since a health food is often used as an aqueous solution, a water-soluble one is preferable. Further, this phytic acid and its salts are contained in a large amount in the fermentation broth, and when converted from the phosphorus concentration, 6 to 7 parts by weight of the phytic acid equivalent is contained per 100 parts by weight of the evaporation residue. There is. Therefore, this fourth book
It is considered that even if the phytic acid or the like contains a predetermined amount of the green tea extract as in the invention, the same effect can be surely obtained as in the case of the first invention.

[0011]

The present invention will be described in detail with reference to the following examples. (1) Production of Fermentation Solution and Preparation of Health Food Test Product First, a fermentation solution is produced by the following method. That is,
Defatted rice bran; 30 kg, soybean meal (or defatted soybean); 3 k
g, bittern, 5 kg, water; 500 kg were used. In addition, p
H is around 9. The above medium was sterilized at 121 ° C. for 30 minutes, then cooled, and then inoculated with 0.05 Kg of natto bacterium (manufacturer; Naruse Fermentation and Chemical Research Institute), aeration and stirring at 40 to 45 ° C. for about 48 hours. And cultured.

Thereafter, this culture was squeezed and filtered, treated with activated carbon and perlite to deodorize and decolorize, and an almost transparent fermentation liquor (fermented extract) was obtained. As the activated carbon, various types such as powdered activated carbon (activated carbon S, activated carbon K, etc.) and granular activated carbon (activated carbon SG, etc.) can be used, and as the perlite, “Perlite No. 4180” (Daikaline Orient Co., Ltd.) Manufactured) was used. This fermented liquid is a colorless or pale yellow transparent liquid, has a flavor of fermentation, and has a water content of 92 to 94% (according to a normal pressure heat drying method).
General live bacteria, Escherichia coli, mold, yeast, arsenic, and heavy metals were not detected.

As the test product 1 of this health food, 3 parts by weight of the green tea extract (methanol or ethanol extract, solid content concentration; 47: 100 parts by weight of the fermentation liquid) was used.
˜48% by weight, specific gravity; 1.07 to 1.09) (evaporation residue ratio: fermentation liquor content / extracted extract content = 100:
20) was used. The solid content concentration was determined as a Brix value, and the concentration of ethanol and glycerin (mainly ethanol) was about 50% by weight, and the concentration of water was about 2.6% by weight. Moreover, it is considered that this green tea extract contains a considerable amount of flavonoids.

(2) Performance Evaluation (A) SOD Action Under the following conditions, a test on H ₂ O ₂ (Table 1,
Table 2), a test for OH (Table 3), and a test in the O ₂ -xanthine system (Table 4) were performed, and the results are shown in each table. As a test product, a 5-fold diluted solution with water was used as the test product 1.

[0015]

[Table 1]

Table 1 --------------------------------------------- [Example 1] H ₂ O ₂ --- −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−− 0 min 10 min Average / min PLCO mol / min −−−−−−−−− −−−−−−−−−−−−−−−−−−−−−−−−−− control product 5 50 2.5 348 −−−−−−−−−−−−−−−−− −−−−−−−−−−−−−−−−− Test product 1 75 73 0.2 28 −−−−−−−−−−−−−−−−−−−−−−−− −−−−−−−−−−−

[0017]

[Table 2]

Table ₂ --- ------------------------------------- [Example 2] H ₂ O ₂ --- −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−− 0 min 10 min Average / min PLCO mol / min −−−−−−−−− −−−−−−−−−−−−−−−−−−−−−−−−−− control product 75 23 5.2 723 −−−−−−−−−−−−−−−− −−−−−−−−−−−−−−−−−− Test product 1 75 70 0.5 70 −−−−−−−−−−−−−−−−−−−−−−−− −−−−−−−−−−−

[0019]

[Table 3]

Table 3 −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−− OH sample −−−−−−−−−−−− ---------------- [Example 1] [Example 2] --------------------------------------- --- STIMULATER / INCUBATION 40 minutes 40 minutes ----------------------------------- −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−− Test product 1 153.0 1.87 −−−−−−−−− −−−−−−−−−−−−−−−−−−−−−−−−−

[0021]

[Table 4]

Table 4 −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−−− O ₂ -xanthine system ---------- −−−−−−−−−−−−−−−−−−−−−−−−−− Sample O. D. (NM) nM / min −−−−−−−−−−−−−−−−−−−−−−−− C (2 min-1 min) C-Black −−−−−−−−−− −−−−−−−−−−−−−−−−−−−−−−−− control product 0.0251 1.190 −−−−−−−−−−−−−−−−−−− --------------------- Test product 1 0.0244 0.0205 0.972 ------------------------------ −−−−−−−−−−

As shown in Tables 1 to 4 above, this test product 1
Indicates that the SOD action is superior to that of the control product. From the above, the SOD activity was 46
It can be said that it is 0 unit / ml (1 cup 10 ml).

(B) Fluctuations in blood pressure during elevated blood pressure in rats Under the following conditions, 100 parts by weight of the fermentation broth was mixed with 1.5 parts by weight of the green tea extract to give a test product 2. The change in blood pressure due to the administration of this test product 2 was evaluated. The result is shown in FIG.

[Test conditions] Animal: rat; 3 males for control, 4 males for test product,
7 in total. The age of this rat is 6 weeks, and its blood pressure is 150 mmHg or higher. It should be noted that this rat has a genetic property that blood pressure increases from 3 weeks of age (hereinafter referred to as SH
Called R. ). Test administration method: Free intake by water bottle. Indoor: Temperature 23 ± 1 ° C, humidity 50% ± 10%. Beverage: Glucose and citric acid were added to a 10% solution of the test product 2 (corresponding to SOD) to make it easy to drink. Blood pressure measurement; once every 2 weeks. Feed used: Common feed (“MF” manufactured by Oriental Yeast Co., Ltd.) was used in all cases. The results show that the use of this test product 2 has an effect of suppressing an increase in blood pressure as compared with the control group.

(C) Fluctuation of blood pressure in the hypertensive completion stage of rats The fluctuation of blood pressure due to administration of Test Product 2 was evaluated under the same conditions as above except the following conditions. The result is shown in FIG. [Test Conditions] Animal: rat (above SHR); control: 4 males, test product: 4 males, 8 in total. And the age of this rat is 12
At 15 weeks of age, its blood pressure is 180 mmHg or higher.
The other conditions are the same as above. According to this result, as shown in FIG. 2, it has an effect of suppressing hypertension and, at the same time, an excellent suppressing effect with the passage of administration time.

(D) Monitor test (1) Regarding the constipation-improving effect, a monitor test was conducted as follows, and the following good results were obtained. This test method is intended for 50 women with constipation (the number of valid answers is 49), and 5 ml of the stock solution (fermented solution) is ingested within 30 minutes of waking up. As a result, 38 women had bowel movements on the first day and all 49 women had bowel movements on the second and third days.

(2) Further, 60 parts by weight of the fermentation broth, 1.2 parts by weight of the green tea extract and 14 parts by weight of oligosaccharide,
The following good results were obtained when a monitor test was conducted in which a test product (portion containing 10 ml) mainly composed of purified water was used for humans. A (58 years old, female) [Symptoms before the start of the monitor] His face was dark, the climbing of stairs was very difficult, his blood pressure was 165, and he was constantly taking a depressant. [Results after 2 drinks daily] My legs became lighter in about 1 week. The antihypertensive drug was stopped and the blood pressure became 130 in about 3 weeks. After two months, my face turns white so much that I can't see it. The 10-year-old started to look younger.

B (70 years old, male) [Symptoms before the start of the monitor] Even in everyday life, my cardiopulmonary function was weak, so I felt like I was out of breath when I climbed the stairs and dragged my legs. [Results after 3 daily drinks (morning, noon, evening)] In about 2 weeks, the body begins to feel light. After about a month, I can't breathe out even when I climb the stairs. Four months later, when I was asked to see cardiopulmonary function at the hospital, it was much better. For the first time this winter, I was fine without having to be hospitalized without catching a cold.

C (52 years old, female) [Symptoms before starting monitoring] He was suffering from diabetes and constipation due to high blood pressure. The blood pressure is 180 at the top and 95 at the bottom. [Results after 2 daily drinks (for 2 months) then 1 daily drink]
From the 4th day, urine is about twice as much as before and my body is feeling better. The stools will also appear once every three days. Then every two days. Two months later, my doctor tells me that I don't have to take blood pressure lowering drugs. His blood pressure was 145 on the upper side and 78 on the lower side, and he no longer needed medicine.

D (23 years old, female) [Symptoms before the start of monitoring] Constipation was severe 1 since childhood.
She had a flight once every two weeks from the 0th day. There were always small pimples on the face, and the skin was dry.
It was rather slender, but the lower abdomen was protruding like an infant figure, and I was considerably worried. [Results after 5 drinks for the first 2 days and 2 drinks every day] The following morning, there was a bowel movement. There are daily flights than that. After a month and a half, my face was completely smooth and my skin was shiny. The makeup glue has improved very much. E (70 years old, female) [Symptoms before starting monitoring] He was hospitalized for high blood pressure of 200. [Results after 2 daily drinks (morning and evening)] Blood pressure dropped in about 10 days and I was able to be discharged from the hospital. Currently 140.

F (68 years old, female) [Symptoms before starting monitoring] I was tired and tired, and my body was also stiff. [Results after 2 daily drinks (morning and evening)] Moisturized skin and improved makeup paste from the 2nd day. I was less tired than in the second week and I started to move as I wanted. G (45 years old, male) [Symptoms before starting monitoring] Diabetes was frequently hospitalized for 2 to 3 weeks, and the liver was hurt during the treatment. [Results after 2 daily drinks] After 2 months, my doctor told me that my liver had completely improved, and after 4 months I had better diabetes, and I was able to get back to work by returning to work. There is no need for hospitalization.

(E) Summary of Effects of Examples As described above, the present fermentation liquor is safe because harmful bacteria and heavy metals are not recognized, and it is a natural fermented product of completely natural raw materials. As shown in the above results, the SOD action is excellent, and thus the blood pressure suppressing effect is excellent as described above. In addition, since it is excellent in this SOD action,
In addition to the blood pressure suppressing effect, it can be said that the effects resulting from this SOD action are expected in the same manner. Furthermore, the present inventors believe that this fermented liquid has an action of effectively incorporating the green tea extract into the body. In this regard, it is necessary to wait for further detailed research, but in any case, if this fermentation broth and green tea extract are used in combination, the SOD action is excellent and it can be safely consumed by humans, and it is difficult for the human body. Clearly useful.

The present invention is not limited to the specific examples described above, and various modifications may be made within the scope of the present invention depending on the purpose and application.

[0035]

Since the present health food is excellent in SOD action, it is excellent in the effects resulting from the SOD action (for example, blood pressure suppressing effect). Therefore, by using the present fermented food, various diseases caused by active oxygen, for example, diseases caused by blood flow disorder (myocardial infarction, stroke, high blood pressure, menstrual pain, stiff shoulder, neuralgia, back pain, hangover, etc.), adult diseases, Those that are excellent in the treatment and improvement of internal diseases (cancer, nephritis, hepatitis, diabetes, etc.), beauty and skin diseases (stains, freckles, rough skin, colds, constipation, wrinkles, atopic dermatitis, etc.) it is conceivable that.

In particular, since the fermented liquor used as the main component in the fermented foods according to the first to third inventions is a natural fermented product made of completely natural raw materials, harmful bacteria and heavy metals are not recognized and it is safe. Besides, since it is originally made from rice bran and soybean products that are useful as health foods, and contains useful nitrogen compounds (amino acids etc.), ash, phosphorus compounds, etc., it is a very well-balanced health food. .
Furthermore, because the green tea extract is also a natural product,
The safety of fermented foods containing this is also excellent.

[Brief description of drawings]

FIG. 1 is a graph showing changes in blood pressure during SHR blood pressure elevation in an example.

FIG. 2 is a graph showing fluctuations in blood pressure in the SHR hypertension completion period in Examples.

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[Procedure amendment]

[Submission date] January 27, 1993

[Procedure Amendment 1]

[Document name to be amended] Statement

[Name of item to be amended] Title of invention

[Correction method] Change

[Correction content]

[Title of Invention] Health and beauty having SOD action
Enhancer, blood pressure suppressor and constipation improver

[Procedure Amendment 2]

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[Name of item to be amended] Claims

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[Claims]

[Procedure 3]

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[Correction target item name] 0001

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[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a health device having an SOD function.
The present invention relates to an agent for promoting beauty and beauty, an agent for suppressing blood pressure, and an agent for improving constipation .
The present invention relates to various diseases caused by active oxygen (O ₂ ⁻ ), for example, diseases caused by blood flow disorder (myocardial infarction, stroke,
High blood pressure, menstrual cramps, stiff shoulders, neuralgia, backache, hangover, etc., adult diseases / medical diseases (cancer, nephritis, hepatitis, diabetes, etc.), beauty / skin diseases (stains, freckles, rough skin, coldness, constipation, wrinkles, atopic nature) It is widely used for the treatment and improvement of dermatitis, etc.).

[Procedure amendment 4]

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[0003]

PROBLEM TO BE SOLVED BY THE INVENTION Safe and SOD action
(The SOD action here is to decrease the active oxygen concentration.
It is thought that it is caused not only by the action but also by this
It also includes the action of preventing and improving various diseases. ) Is very useful for human health and beauty, and there is a great need for it. In particular, in a situation where stress is high and many diseases are occurring as in recent years, the necessity thereof is great.

[Procedure Amendment 5]

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[0004] The present invention, health and beauty of using rice bran acids and soybeans as raw material, and having a superior SOD activity safe
It is an object to provide a promoter, a blood pressure suppressor and a constipation improver, and further a health / beauty enhancer having an SOD action, which comprises at least one of phytic acid and a salt thereof and a green tea extract. .

[Procedure correction 6]

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[Name of item to be corrected] 0005

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[0005]

From the above viewpoints, the inventors of the present invention may not use a safe fermented product as the health / beauty enhancer having SOD activity. As a result of intensive studies on various matters, etc., the present invention was completed by discovering that the objective can be achieved by adding a predetermined additive to the fermentation liquid obtained by fermenting and filtering the raw material. It came to. The health / beauty enhancer having an SOD effect of the first invention is a fermentation liquor produced by inoculating Bacillus subtilis into a medium containing rice bran , soybeans and water , culturing and filtering, or an evaporation residue of the fermentation liquor. In addition, the green tea extract extract or the evaporation residue of the green tea extract extract is contained in each minute. The sixth aspect of the invention is to increase the health and beauty of the first aspect.
It is a drug that utilizes a progressing agent as a blood pressure suppressant.
The 11th invention uses the health and beauty promoting agent as a constipation improving agent.
It is a thing.

[Procedure Amendment 7]

[Document name to be amended] Statement

[Correction target item name] 0006

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The "green tea extract" is obtained by extracting tea leaves using methanol, ethanol, ethyl acetate and the like (particularly methanol and ethanol) as a solvent, and the concentration of the extracted components is about 5 to 50% by weight. be able to. And, as shown in the second invention, the mixing ratio of the green tea extract can be 1 to 50 parts by weight in terms of the evaporation residue, based on 100 parts by weight of the evaporation residue of the fermentation liquid. . This is because if it is less than 1 part by weight, the effect cannot be expected sufficiently, and if it exceeds 50 parts by weight, it becomes difficult to taste and drink. Also, the third and the third
In the eighth and thirteenth inventions, the green tea extract is
Tanol, ethanol, or methanol / ethanol mixture
It was extracted with a combined solution to make it water-soluble.

[Procedure Amendment 8]

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[Correction target item name] 0007

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Health and beauty having the SOD action of the fourth invention
The volume enhancer is obtained by inoculating Bacillus subtilis in a medium containing rice bran , soybeans and water , culturing, culturing, and producing a fermentation liquor produced by filtration or an evaporation residue of the fermentation liquor, which comprises flavonoids. Characterize. The ninth invention is the health and beauty of the fourth invention.
It uses a volume enhancer as a blood pressure suppressant.
In the 14th invention, the health and beauty promoting agent is used as a constipation improving agent.
I used it. The "flavonoid" is widely distributed in the plant kingdom, the two phenyl groups are bonded across the three carbon atoms of the pyran ring, or close to it the structure, so-called C ₆ -C ₃ -C ₆ It is a group of compounds consisting of carbon skeleton. This flavonoid is contained in 13 to 14% by weight based on the evaporation residue in the green tea extract. The blending ratio of the flavonoid can be about 0.1 to 7 parts by weight with respect to 100 parts by weight of the fermentation broth (for example, when the extract concentration is 40 to 50% by weight). This is because if the amount is less than 0.1 part by weight, the effect cannot be expected sufficiently, and if it exceeds 7 parts by weight, it becomes difficult to taste and drink.

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The "fermentation solution" may be a solution obtained by culturing the cultured culture fermentation solution as it is, a solution that has been subjected to post-treatment such as decolorization, or a concentrated solution obtained by concentrating the solution. As described above, the evaporation residue (solid content) of this fermentation liquid may be used. Has the SOD function of the fifth invention
The health / beauty enhancer is characterized by containing at least one of phytic acid and a salt thereof and a green tea extract (or an evaporation residue of the green tea extract). Also,
A tenth invention is a blood pressure suppressor for the health and beauty enhancer of the fifth invention.
It is used as an agent, and the 15th invention is
It is an application of a health and beauty enhancer as a constipation improving agent.

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[Correction target item name] 0010

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The above-mentioned "phytic acid" may be synthetic phytic acid or phytic acid extracted and isolated. Also,
As the “salt”, a non-toxic salt is used, and a salt with a metal, an organic base, a basic amino acid, an organic ester residue, etc. is used, and examples thereof include potassium, sodium, arginine,
It may be a salt of ornithine, lysine, histidine, glucamine, monoethanolamine or the like. Since this phytic acid exhibits acidity, a salt thereof having a pH of about 6 to 8 is usually used. It does not matter whether this salt is water-soluble or not,
Since a health / beauty enhancer or the like is often used as an aqueous solution, a water-soluble one is preferable. Further, this phytic acid and its salts are contained in a large amount in the fermentation broth, and when converted from the phosphorus concentration, 6 to 7 parts by weight of the phytic acid equivalent is contained per 100 parts by weight of the evaporation residue. There is. Therefore, as in the fifth, tenth, and fifteenth inventions, even if a predetermined amount of green tea extract is contained in phytic acid or the like, as in the case of the first invention, the same effect is surely obtained. It is considered to be a thing.

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[0011]

The present invention will be described in detail with reference to the following examples. (1) Manufacture of fermented liquids and health foods (health and beauty enhancers, etc.
Say. ) Preparation of test article First, a fermentation broth is produced by the method described below. That is,
Defatted rice bran; 30 kg, soybean meal (or defatted soybean); 3 k
g, bittern, 5 kg, water; 500 kg were used. In addition, p
H is around 9. The above medium was sterilized at 121 ° C. for 30 minutes, then cooled, and then inoculated with 0.05 Kg of natto bacterium (manufacturer; Naruse Fermentation and Chemical Research Institute), aeration and stirring at 40 to 45 ° C. for about 48 hours. And cultured.

[Procedure Amendment 12]

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Thereafter, this culture was squeezed and filtered, treated with activated carbon and perlite to deodorize and decolorize, and an almost transparent fermentation liquor (fermented extract) was obtained. As the activated carbon, various types such as powdered activated carbon (activated carbon S, activated carbon K, etc.) and granular activated carbon (activated carbon SG, etc.) can be used, and as the perlite, “Perlite No. 4180” (Daikaline Orient Co., Ltd.) Manufactured) was used. The fermented liquid is a transparent colorless liquid or pale yellow, there are fermentation incense昧water 92-94% (by normal pressure heat drying method.), Li
Approximately 300 to 400 mg / 100 g (vanado molybdenum
Depends on the analysis method. ) , General live bacteria, Escherichia coli, mold, yeast and arsenic, lead, cadmium and tin were not detected.

[Procedure Amendment 13]

[Document name to be amended] Statement

[Correction target item name] 0033

[Correction method] Change

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(E) Summary of Effects of Examples As described above, the present fermentation liquor is safe because harmful bacteria and heavy metals are not recognized, and it is a natural fermented product of completely natural raw materials. As shown in the above results, the SOD action is excellent, and thus the blood pressure suppressing effect is excellent as described above. In addition, since it is excellent in this SOD action,
In addition to the blood pressure suppressing effect, it can be said that the effects resulting from this SOD action are expected in the same manner. Furthermore, the present inventors believe that this fermented liquid has an action of effectively incorporating the green tea extract into the body. In this regard, it is necessary to wait for further detailed research, but in any case, if this fermentation broth and green tea extract are used in combination, the SOD action is excellent and it can be safely consumed by humans, and it is difficult for the human body. Clearly useful. Also, the fermentation liquid
Both the green tea extract and the green tea extract are water-soluble, so
Dissolve it in water as shown and use it as an aqueous beverage, food, etc.
Can be used and therefore is well absorbed by the body.
Get Furthermore, as shown in the monitor test of (D),
(The small container shape containing the fresh milk
Say. ) Beverages containing this test product (aqueous solution) in a container
Various aqueous products as tested as (food)
(Beverages, drinks, etc.) is also easy.

[Procedure Amendment 14]

[Document name to be amended] Statement

[Correction target item name] 0034

[Correction method] Change

[Correction content]

It should be noted that the present invention is not limited to those shown in the above non-limiting examples, and various modifications can be made within the scope of the present invention depending on the purpose and application. That is,
Health promoting agent consisting of the above fermented liquid etc. and green tea extract etc.
Etc. are usually in the form of an aqueous solution as shown in the above examples.
It is a liquid such as undiluted liquid, but not limited to this,
A powder product in which a liquid-absorbent powder is impregnated with a promoting agent, or granulated.
Granulated products, tablets that are mixed with other powder components (fillers, etc.)
Agent, microcapsule, soft capsule, hard capsule
Fill puccel, candy, jelly, biscuit, etc.
Can be blended. Also these
Product form that is filled with a prescribed container with an aqueous solution, powder product, etc.
State, or use it alone or other agents (aqueous solutions, oil
It does not matter whether it is a liquid or a powder. ) To use
Whether to do is not particularly limited, and for example, the above-mentioned embodiment
It can be a potion type as mentioned in
May be filled in a powder container or a powder product in a stick container
It may be filled in a (bag) or, in addition, conventional soft drinks
Mix and disperse in water, drinks, dairy products, oil-based products, etc.
You may use it. This dispersion is a water-in-oil type, oil-in-water
Any type.

[Procedure Amendment 15]

[Document name to be amended] Statement

[Correction target item name] 0035

[Correction method] Change

[Correction content]

[0035]

EFFECTS OF THE INVENTION Since the health and beauty promoting agent is excellent in SOD action, it is also excellent in the effects resulting from the SOD action (for example, blood pressure suppressing effect). Therefore, by using this health and beauty enhancer , various diseases caused by active oxygen, for example,
Diseases caused by blood flow disorders (myocardial infarction, stroke, high blood pressure, menstrual cramps, stiff shoulders, neuralgia, back pain, hangover, etc.), adult diseases / internal diseases (cancer, nephritis, hepatitis, diabetes, etc.), beauty / skin diseases (stains, freckles) , Rough skin, chills, constipation, wrinkles, atopic dermatitis, etc.), etc., and is considered to exhibit excellent effects. In addition, the blood pressure inhibitor of the present invention and
Constipation improvers improve blood pressure control and bowel movements, respectively.
Excellent effect. In addition, it is used for water-soluble health and beauty enhancers.
If it is a water-soluble beverage, food, etc.
As its application field widens.

[Procedure Amendment 16]

[Document name to be amended] Statement

[Correction target item name] 0036

[Correction method] Change

[Correction content]

Further, in particular, the fermented liquor used as the main component in the present invention is a natural fermented product of a completely natural raw material, so that harmful bacteria and heavy metals are not recognized, and it is safe and originally promotes health and beauty. It is a very well-balanced health and beauty enhancer since it is made from rice bran and soybean products which are useful as a raw material and contains useful nitrogen compounds (amino acids etc.), ash and phosphorus compounds. Furthermore, since the green tea extract is also a natural product, the safety of health and beauty enhancer containing it is excellent. ─────────────────────────────────────────────────── ───

[Procedure amendment]

[Submission date] May 7, 1993

[Procedure Amendment 1]

[Document name to be amended] Statement

[Name of item to be corrected] 0023

[Correction method] Change

[Correction content]

As shown in Tables 1 to 4 above, this test product 1
Indicates that the SOD action is superior to that of the control product. From the above, the SOD activity was 46
It can be said that it is 0 unit / ml (1 cup 10 ml). Furthermore, the following tests were conducted for the synergistic effect of the fermented liquid and the green tea extract. That is, each sample (No. A to C) having the following blending ratio was prepared. SOD activity of each of the above samples was measured by the following measurement method [SOD activity measurement method] This method is a method using an EPR (electron spin resonance) device, and recently, at the Japan Food Analysis Center. Test item: This is a method that has come to be performed as "superoxide scavenging activity". That is, first, to 1 g of each homogenized sample, 0.1 M phosphate buffer solution (p
H7.8) is added and extraction is sufficiently performed to obtain an extract. Then, the following reagents are mixed. 2 mM hypoxanthine / phosphate buffer 50 μl 5.5 mM DETAPAC (diethnetr
iaminepentaacetic acid) / phosphate buffer 35 μl extract or standard SOD (superoxide dismutase) 50 μl DMPO (5,5-dimethyl-1-pyrro)
line-1-oxide) 15 μl 0.4 units / ml xanthine oxidase / phosphate buffer 50 μl Then, this mixed solution is sucked into a special flat cell (manufactured by JEOL Ltd., capacity: about 130 μL), and ESR device (JEOL) (Manufactured by JES-FR80, manufactured by Co., Ltd.), the sweep was started 40 seconds later, and the measurement was performed under the ESR operating conditions shown below. In addition, this SOD activity measurement is a measurement of the solution one day after the sample preparation. [ESR operation conditions] temperature room temperature microwave output 8 mW magnetic field 334.7 mT ± 5 mT modulation 100 kHz, 0.79 × 0.
1 mT Amplification factor 3.2 × 100 Response time 0.1 sec Sweep time 2 min Then, the ratio between the peak height of Mn ^{2+ and} the peak height of the measurement signal is obtained. Then, the standard SOD (0-15 unit
s / ml), a calibration curve was prepared, and the values obtained from the extract were interpolated to calculate the erasing activity in the sample. The results are as follows. The unit is UNIT / g. "Activity additive theoretical value"
Is the sample No. It is simply calculated by using each SOD activity value of A and B. No. including the fermented liquid and the green tea extract In C, the actual SOD activity value is 1.15 times the active additive theoretical value, which indicates that there is a synergistic effect.

Claims (4)

[Claims] 1. A fermentation liquor produced by inoculating a culture medium containing rice bran and soybeans with Bacillus subtilis, culturing and filtering, or an evaporation residue of the fermentation liquor, or a green tea extract or an evaporation of the green tea extract. A health food having an SOD action, which is characterized by containing a residue. 2. The green tea extract is 1 in terms of the evaporation residue, based on 100 parts by weight of the evaporation residue of the fermentation liquor.
The health food having an SOD action according to claim 1, which is mixed in an amount of 50 parts by weight. 3. A fermentation liquor produced by inoculating a culture medium containing rice bran and soybeans with Bacillus subtilis, culturing and filtering the mixture, or an evaporation residue of the fermentation liquor containing flavonoids. A health food having an SOD action. 4. A health food having an SOD action, which comprises at least one of phytic acid and a salt thereof and a green tea extract or the green tea extract.