JP7075177B2 - Cosmetics - Google Patents

Description

The present invention relates to cosmetics, and more particularly to cosmetics useful for improving skin transparency, water absorption ability and water retention ability.

"Decreased transparency" is a phenomenon that appears with age, and the more it appears at the top of women's skin problems, the greater the problem of appearance for women. In addition, since it is one of the visual symptoms that are easily observed by others and directly linked to aging, this measure is desired. There are various causes of decreased transparency, such as decreased blood flow, decreased water content in the stratum corneum, and disturbance of the condition of the stratum corneum on the skin surface. There are countermeasures for each of these causes. For example, in the case of external skin preparations such as cosmetics, attempts have been made to increase blood flow in the skin and improve the transparency of the skin by applying a blood circulation promoting agent. (For example, see Patent Document 1 below). Further, it is said that the factor related to the transparency of the skin is improved by improving the water content of the stratum corneum by applying a moisturizer or the like (see, for example, Patent Document 2 below). In addition, there are external skin preparations that improve the transparency of the skin by suppressing glycation of the stratum corneum with AGEs decomposition products (see, for example, Patent Document 3 below).

Japanese Unexamined Patent Publication No. 2015-86160 Japanese Unexamined Patent Publication No. 2002-255827 Japanese Unexamined Patent Publication No. 2011-246354

However, the methods of Patent Documents 1 and 2 also have a temporary effect of improving the transparency, and do not essentially improve the effect. The method of Patent Document 3 is useful for yellowing of the skin, but contributes little to the improvement of transparency. Therefore, there has been a demand for skin cosmetics that essentially improve the skin and improve the transparency.

As a result of intensive research to solve the above problems, the present inventors combine a filaggrin production promoter that promotes the health of the stratum corneum with a tannin-containing plant extract that prepares a favorable maturation environment for the stratum corneum. Therefore, they have found that they are excellent in improving the essential transparency of the skin, and have completed the present invention.

That is, the present invention relates to the following techniques.
(1) A cosmetic containing the following (A) and (B).
(A) Phyllagulin production promoter (B) Tannin-containing plant extract (2) The phyllagulin production promoter of (A) is selected from one or more of the Liliaceae Ruscus genus, the Liliaceae Hauchiwamame genus, and the Polygonaceae Knotweed genus. The cosmetic according to (1), which is a plant extract.
(3) The tannin-containing plant extract of (B) is a plant extract selected from one or more of the genus Olive of the family Oleaceae, the genus Guava of the family Oleaceae, the genus Guava of the Labiatae family, the genus Moxa of the family Asteraceae, and the genus Eriobotrya of the family Rosaceae. The cosmetic according to any one of (1) to (2).
(4) The cosmetic according to any one of (1) to (3), wherein the tannin-containing plant extract of (B) is a leaf extract.
(5) The cosmetic according to any one of (1) to (4) for improving skin transparency.
(6) The cosmetic according to any one of (1) to (4) for improving water absorption ability.
(7) The cosmetic according to any one of (1) to (4) for improving water retention ability.
(8) The cosmetic according to any one of (1) to (4), which has an effect of promoting metabolism of stratum corneum cell protein.
(9) The cosmetic according to any one of (1) to (8), which further contains a phospholipid as a component (C).

According to the present invention, the skin transparency can be improved by applying a cosmetic containing (A) a filaggrin production promoter and (B) a tannin-containing plant extract as active ingredients to the skin.

It is a schematic diagram of the apparatus which measures the skin transparency. It is a figure which shows the concept of the parameter used for the evaluation of the skin transparency. This is the result of measuring the amount of blue internal reflected light before and after the continuous application of Example 4. This is the result of evaluating the distribution of desmoglein in the outermost layer of the horny layer before and after the continuous application of Example 4.

Hereinafter, suitable embodiments for carrying out the present invention will be described. It should be noted that the embodiments described below show an example of a typical embodiment of the present invention, and the scope of the present invention is not narrowly interpreted by this. When the numerical range is represented by "X to Y" in the description of the present invention, the range includes the values X and Y at both ends. Hereinafter, unless otherwise specified,% means mass%.

Component (A): Filaggrin production promoter The filaggrin production promoter in the present invention is not only one that promotes the production of filaggrin itself, but also one that promotes the production of profilaggrin and the presence of profilaggrin mRNA in epidermal keratinocytes, and the filaggrin coding gene. Including those that promote existence.

Here, the filaggrin production promoter is not particularly limited as long as it has the above-mentioned effect, but if it is a plant extract, it is excellent in safety and preferable. It is more preferable if it is a plant extract selected from one or more species of the genus Ruscus of the family Liliaceae, the genus Lupine of the family Leguminosae, and the genus Persicaria longise of the family Polygonaceae.

Butcher's Bloom (scientific name: Ruscus aculeatus) is an example of a plant belonging to the genus Butcher's-broom in the family Liliaceae. It is distributed in Southern Europe and North Africa along the Mediterranean coast and is easily available from these regions. The site that can be used as an extraction raw material is not particularly limited and may be appropriately selected depending on the purpose. For example, leaves, branches, stems, flowers, buds, fruits, bark, roots, rhizomes, above-ground parts or these. Mixtures are mentioned, of which rhizomes are preferred.

More than 200 species of lupines of the genus Lupinus are distributed in the Mediterranean coastal regions, the Americas, South Africa, etc., and any of them can be used, but yellow lupine (scientific name: Lupinus luteus) and white flower lupine (scientific name: Lupinus albus). Is preferable, and white flower lupine is particularly preferable. The site that can be used as an extraction raw material is not particularly limited and may be appropriately selected depending on the purpose. For example, leaves, branches, stems, flowers, buds, fruits, bark, roots, rhizomes, above-ground parts or these. Mixtures are mentioned, of which seeds are preferred.

The genus Persicaria longise can be further classified into more detailed categories such as Persicaria nepalis, Persicaria filiformis, and Persicaria longise, but any of them may be used. Of these, the Sanaetade section is preferable. Specific examples of plants in the section of Sanaetade include Yanagitade (scientific name: Persicaria hydropiper), Sanaetade (scientific name: Persicaria lapathifolia), Knotweed (scientific name: Persicaria longiseta), Redshank (scientific name: Persicaria maculosa), Mizosoba (scientific name: Persicaria thun). (Tadeai) (scientific name: Persicaria tinctoria), etc., but is not limited to this. Water peppers and eyes are preferable, and eyes are particularly preferable. The site that can be used as an extraction raw material is not particularly limited and may be appropriately selected depending on the purpose. For example, leaves, branches, stems, flowers, buds, fruits, bark, roots, rhizomes, above-ground parts or these. Mixtures are mentioned, and among these, above-ground whole plants are preferable.

Although the details of the substances contained in the extract from the above-mentioned extraction raw materials are unknown, an extract having these actions can be obtained from the above-mentioned extraction raw materials by an extraction method generally used for extracting natural products. ..

For example, an extract having a filaggrin production promoting action can be obtained by drying the above-mentioned extraction raw materials as necessary, pulverizing them as they are or using a coarse crusher, and subjecting them to extraction with an extraction solvent. Drying may be carried out in the sun or by using a commonly used dryer. Further, it may be used as an extraction raw material after being subjected to pretreatment such as degreasing with a non-polar solvent such as hexane. By performing pretreatment such as degreasing, the extraction treatment using the polar solvent of the extraction raw material can be efficiently performed. Before and after extraction, decomposition by an enzyme, fermentation by a microorganism such as yeast, or the like may be performed.

As the extraction solvent, it is preferable to use a polar solvent, for example, water, a hydrophilic organic solvent and the like, and these are used alone or in combination of two or more at room temperature or a temperature below the boiling point of the solvent. Is preferable.

Water that can be used as an extraction solvent includes pure water, tap water, well water, mineral spring water, mineral water, hot spring water, spring water, fresh water, and the like, as well as those subjected to various treatments. Treatments applied to water include, for example, purification, heating, sterilization, filtration, ion exchange, osmoregulation, buffering and the like. Therefore, the water that can be used as the extraction solvent in the present invention includes purified water, hot water, ion-exchanged water, physiological saline, phosphate buffer, phosphate buffered saline and the like.

Hydrophilic organic solvents that can be used as the extraction solvent include lower aliphatic alcohols having 1 to 5 carbon atoms such as methanol, ethanol, propyl alcohol and isopropyl alcohol; lower aliphatic ketones such as acetone and methyl ethyl ketone; 1,3-butylene. Examples thereof include polyhydric alcohols having 2 to 5 carbon atoms such as glycol, propylene glycol and glycerin.

These plants can be used alone or in combination of two or more. Although the amount of the plant extract is not particularly specified, it is 0.000005 to 0.5% by mass, preferably 0.00005 to 0.05% by mass, and particularly preferably 0.0005 to 0.005 in terms of solid content in the cosmetic. It is mass%.

Ingredient (B): Tannin-containing plant extract Tannin is a general term for water-soluble compounds derived from plants that react with proteins, alkaloids, and metal ions to form a sparingly soluble salt. It is a complex aromatic compound with a phenolic hydroxy group. Tannins include both hydrolyzable tannins and condensed tannins. Hydrolyzable tannins mainly refer to glucose bound to gallic acid by an ester bond, and are hydrolyzed to gallic acid and glucose. Condensed tannin is a polymer formed by binding a number of flavonoids. Since it is a carbon-carbon bond, hydrolysis does not occur and it is insoluble in water. In the present invention, any of them may be used, and can be used without being limited by the degree of polymerization, origin and the like.

Although the effect varies depending on the type of tannin, it is generally said to have an antibacterial action and a deodorant action in addition to a convergent property (the property of binding to proteins and the like to tighten tissues). It was not known that these astringent effects lead to skin transparency. The tannin-containing plant extract may be any tannin-containing plant extract, and the site and extraction method are not limited, but it is desirable to prepare the tannin-containing plant extract so as to contain a large amount of tannin.

The component (B) tannin-containing plant extract used in the present invention is not particularly limited as long as it is an extract from a plant containing tannin. Extracts from the genus Tannin of the family Asteraceae, the genus Eriobotrya of the family Rosaceae, etc. can be mentioned. The olive extract that can be used in the present invention is an extract of a plant belonging to the genus Olive of the family Oleaceae. Specific examples of the olive genus plant of the Oleaceae family include olive (Oleaeuropaea Linne) and its similar species (for example, Olea welwitschii, Olea paniculata, etc.), and there are more than 500 varieties. Typical examples are Nevadi Bronco, Manzanillo, Picual, Hoji Blanco, Albekina, Catamara, Koroneiki, Pitchorine, Paragon, Wagaberdal, Mission, Washington, West Australia Mission, South Australia Bendal, Azapa, Barnea, Cornicabra, Goldal, Frantio, Leccino, Cipressino, Lucca, Ascolana Terena, Collegillo, Olive Oro, Black Italian, Collatina, Helena, Rossiora, One Seven Seven, El Greco, Hardy's Mammoth, etc. can be preferably used. The site that can be used as an extraction raw material is not particularly limited and may be appropriately selected depending on the purpose. For example, leaves, branches, stems, flowers, buds, fruits, bark, roots, rhizomes, above-ground parts or these. Examples include mixtures, of which fruits or leaves are preferred, with leaves particularly preferred.

Plants belonging to the genus Guava of the family Guava that can be used in the present invention include, but are not limited to, Sidium (Psidium guajava), Teriha guava (Psidium cattleianum), Strawberry guava (Psidium longifolium), and the like. be able to. It is particularly preferable to use sidium. Sidium is spread from the tropical American region to the tropical and subtropical regions of the world, and is cultivated in parts of Okinawa and Kagoshima regions in Japan, and is called guava. The site that can be used as an extraction raw material is not particularly limited and may be appropriately selected depending on the purpose. For example, leaves, branches, stems, flowers, buds, fruits, bark, roots, rhizomes, above-ground parts or these. Examples include mixtures, of which fruits or leaves are preferred, with leaves particularly preferred.

The plants of the Labiatae genus Skullcap that can be used in the present invention include Skullcap (scientific name: Scutellaria guilielmii A. Gray) Skullcap (scientific name: Scutellaria indica L.), Scutellaria baicalensis Georgi (scientific name: Scutellaria baicalensis Georgi). : Scutellaria lateriflora Carl Linnaeus), Marsh Skullcap (scientific name: Scutellaria galericulata Carl Linnaeus), etc., but any of them can be preferably used. In particular, Scutellaria baicalensis (also known as Ogon) is preferable. The site that can be used as an extraction raw material is not particularly limited and may be appropriately selected depending on the purpose. For example, leaves, branches, stems, flowers, buds, fruits, bark, roots, rhizomes, above-ground parts or these. Examples include mixtures, of which leaves or rhizomes are preferred, with rhizomes particularly preferred.

The plants of the genus Artemisia montana that can be used in the present invention include Nigayomogi (scientific name: A. absinthium), Taragon (scientific name: A. dracunculus) Yomogi (A. princeps), Yamayomogi Artemisia montana, Moukoyomogi Artemisia mongolia, and Kawarayomogi (Kawarayomogi). Scientific name: A. capillaris Thunb.), Artemisia vulgaris, Artemisia vulgaris (scientific name: Artemisia vulgaris), Otokoyomogi (scientific name: A. japonica Thunb.) Nishiyomogi (scientific name: A. indica Willd. Var. Orientalis) Although there are species, any of them can be preferably used. Among them, mugwort, mugwort, and capillary wormwood can be preferably used. The site that can be used as an extraction raw material is not particularly limited and may be appropriately selected depending on the purpose. For example, leaves, branches, stems, flowers, buds, fruits, bark, roots, rhizomes, above-ground parts or these. Examples thereof include whole plants or leaves, and leaves are particularly preferable.

The Rosaceae Eriobotrya plant that can be used in the present invention is an evergreen shrub or tree known to have about 10 species distributed in warm regions of East Asia. Examples of the plant belonging to the genus Eriobotrya of the Rosaceae family include loquat (Eriobotrya japonica (Thunb.) Lindl.), And it is preferable to use this. Examples thereof include leaves, branches, stems, flowers, buds, fruits, bark, roots, rhizomes, above-ground parts or mixtures thereof, of which fruits or leaves are preferred, with leaves particularly preferred.

By using leaves regardless of the type of plant extract, it is possible to obtain an extract having a high tannin content, which is preferable.

As an extraction method, for example, the above-mentioned extraction raw material can be dried as necessary, then pulverized as it is or using a coarse crusher, and extracted with an extraction solvent. Drying may be carried out in the sun or by using a commonly used dryer. Further, it may be used as an extraction raw material after being subjected to pretreatment such as degreasing with a non-polar solvent such as hexane. By performing pretreatment such as degreasing, the extraction treatment using the polar solvent of the extraction raw material can be efficiently performed.

As the extraction solvent, it is preferable to use a polar solvent, for example, water, a hydrophilic organic solvent and the like, and these are used alone or in combination of two or more at room temperature or a temperature below the boiling point of the solvent. Is preferable. Examples of the hydrophilic organic solvent include lower alcohols having 1 to 5 carbon atoms such as methanol, ethanol, propyl alcohol and isopropyl alcohol; lower aliphatic ketones such as acetone and methyl ethyl ketone; 1,3-butylene glycol and propylene glycol. Examples thereof include polyhydric alcohols having 2 to 5 carbon atoms such as glycerin, and a mixed solvent of these hydrophilic organic solvents and water can be used. When a mixed solvent of the water and a hydrophilic organic solvent is used, the amount of lower alcohol is 1 to 20 parts by mass with respect to 10 parts by mass of water, and the amount of lower aliphatic ketone is 10 parts by mass of water. On the other hand, it is preferable to add 1 to 15 parts by mass. In the case of polyhydric alcohol, it is preferable to add 1 to 20 parts by mass with respect to 10 parts by mass of water.

These plants can be used alone or in combination of two or more. Although the amount of the plant extract is not particularly specified, it is 0.000005 to 0.5% by mass, preferably 0.00005 to 0.05% by mass, and particularly preferably 0.0005 to 0.005 in terms of solid content in the cosmetic. It is mass%.

Further, in the present invention, by adding phospholipid as (C), it is possible to improve the stability and feel of the cosmetic. The phospholipid is not particularly limited as long as it is a phospholipid usually used in cosmetics, and one type or a combination of two or more types can be used.

The phospholipid may be a natural product extracted and purified from animals and plants, may be chemically synthesized, and may be processed by hydrogenation, hydroxylation treatment, enzyme treatment or the like. Examples of natural products include soybean-derived phospholipids and egg yolk-derived phospholipids, and examples of processed products include hydrogenated phospholipids and lysophospholipids. More specifically, soybean-derived phospholipids, soybean-derived hydrogenated phospholipids, soybean-derived lysophospholipids, soybean-derived hydrogenated lysophospholipids, egg yolk-derived phospholipids, egg yolk-derived hydrogenated phospholipids, egg yolk-derived lysophospholipids, egg yolk-derived hydrogen. Examples include added lysophospholipids.
Of these, soybean-derived phospholipids or hydrogenated phospholipids are preferable, and soybean-derived hydrogenated phospholipids are particularly preferable.

Commercially available products of (C) include Lecinol S-PIE, Nikkor Resinol S-10, Nikkor Resinol S-10E (all manufactured by Nikko Chemicals), Basis LS-60HR (manufactured by Nisshin Oillio Group), and HSL-70. (Manufactured by YMC), lecithin CLO (manufactured by J-Oil Mills), egg yolk lecithin PL-100P (manufactured by Kewpie) and the like.

The content of (C) used in the present invention in the cosmetics is not particularly limited, and is preferably 0.001 to 10% by mass, more preferably 0.01 to 5% by mass, and particularly preferably 0. It is 05 to 1% by mass.

The mass ratio of the total content of (A) filaggrin production promoter and (B) tannin-containing plant extract used in the present invention to the content of (C) phospholipid is not particularly limited, but ((() A) + (B)): (C) = 1: 5 to 1: 50,000 is preferable, and the range of 1: 50 to 1: 5,000 is more preferable.

In the present invention, (A) a filaggrin production promoter and / or (B) a tannin-containing plant extract can be vesiculated using (C) a phospholipid and used. A vesicle is a closed vesicle having a bilayer membrane structure formed by an amphipathic substance, and is attracting attention as a carrier for a drug delivery system or the like containing an active ingredient due to this specific structure. Since the vesicle has a high retention in the stratum corneum, the active ingredient can act efficiently, and it has attracted particular attention in recent years.

The cosmetics of the present invention include cosmetics, quasi-drugs, and medicated cosmetics. Cosmetics can be prepared in various dosage forms such as solids, semi-solids, and liquids, depending on the purpose of use. More specifically, as basic cosmetics, cleansing, washing pigments, lotions, milky lotions, creams, massage products, pack products, beauty liquids / gels, lip care products, etc .; as base makeup cosmetics, foundations, face powders, makeup bases, etc. Concealers, etc .; Point makeup cosmetics such as lipstick, lip gloss liner, teak products, eye shadows, eye liners, mascara, eyebrow products, etc .; Body cosmetics, soaps, liquid cleaners, sunscreen creams, bathing agents, etc.; for hair As cosmetics or cosmetics for the scalp, shampoo, rinse, hair treatment, hair conditioner, hair tonic, hair restorer, scalp treatment and the like can be used.

The cosmetic of the present invention may contain various functional ingredients that are acceptable as active ingredients or additives of the cosmetic, as long as the effects of the present invention are not impaired. Examples of such ingredients are whitening agents, UV protection agents, antibacterial agents, anti-inflammatory agents, cell activators, reactive oxygen species removers, moisturizers, skin cleansing ingredients, acne and asemo-preventing ingredients. In addition to the agent of the present invention, the cosmetic of the present invention may contain various additives that are acceptable as cosmetics, as long as the effects of the present invention are not impaired. Examples of this include water (purified water, hot spring water, deep ocean water, etc.), surfactants (emulsifiers, solubilizers, suspending agents, stabilizers, etc.), oils, powders, antioxidants, preservatives, etc. There are gelling agents, alcohols, film forming agents, coloring agents, fragrances, deodorants, salts, pH adjusters, refreshing agents, chelating agents, keratolytic agents, enzymes, vitamins and the like.

The cosmetic of the present invention can improve the transparency of the skin. Here, the transparency of the skin includes the transparency inside the skin in the present invention in addition to the general transparency by visual inspection. Any of the cosmetics of the present invention can be improved. The transparency inside the skin specifically indicates the amount of light reflected from the inside when the light is irradiated from the surface of the skin. For this purpose, it is important that light is first absorbed from the surface of the skin to the inside. When the stratum corneum is rough, it is difficult for the irradiation light to enter the skin, and the transparency is reduced. Further, even if the light is absorbed inside the skin, if the internal structure of the skin is disturbed, the light is scattered and absorbed, so that the reflected light is reduced and the transparency is lowered. By improving the transparency inside the skin, the transparency of the entire skin increases, and it is recognized as "beautiful skin".

The cosmetic of the present invention can improve the water absorption ability. Moisture absorption capacity is the ability to guide water to the inside of each layer. Generally, dry skin seems to have high water absorption capacity, but surprisingly, unhealthy skin such as dry skin and rough skin has low water absorption capacity. That is, the higher the value of the water absorption capacity, the better the state of the stratum corneum.

The cosmetic of the present invention can improve the water retention ability. On rough skin, it cannot be retained even if it is forcibly given water, and it releases water over time. Conversely, healthy skin has the ability to retain moisture for long periods of time. The higher the value of the water retention capacity, the higher the water retention capacity and the better the state of the stratum corneum.

The cosmetic of the present invention has an effect of promoting metabolism of stratum corneum cell protein. Examples of the stratum corneum cell protein include stratum corneum cell adhesion protein, stratum corneum substance, and stratum corneum cell constituents, and any of them may be used, and the stratum corneum cell adhesion protein is particularly excellent in the metabolism promoting effect. Epidermal cells play an important role in the normal keratinization of the epidermis and the maintenance of the stratum corneum. It is also known that abnormal cell adhesion causes skin diseases such as keratosis and skin troubles such as acne, dandruff, and desquamation. In addition, it is known that stratum corneum substances and stratum corneum cell constituents are reduced and denatured by aging, and promotion of metabolism is important for beautiful skin and transparency.

Desmoglein 1, 2, 3, 4, desmocollin 1, 2, 3, desmoplakin 1, 2, etc. are known as stratum corneum cell adhesion proteins, and they play important roles in these cell adhesion factors and cytoskeleton. When these are impaired, pathological conditions such as vesicular disease occur. Of these, desmoglein, particularly desmoglein 1, is known to be closely related to the adhesion function of stratum corneum cells or their detachment function. (See, for example, Patent No. 4805794) We found that desmoglein was found on the entire surface of the stratum corneum cells, and that the larger the amount, the more so-called rough skin and the less transparent. It has been found that the cosmetic of the present invention changes the presence of desmoglein by promoting metabolism of proteins in stratum corneum cells. Moreover, it was found that the skin in a state where such desmoglein is present only in the marginal part of the stratum corneum has a high transparency. It was not known at all that the skin transparency could be evaluated by the presence of such desmoglein in the stratum corneum.

Hereinafter, the present invention will be described in more detail with reference to Examples, Comparative Examples, and Production Examples, but the scope of the present invention is not limited thereto.

[Preparation Example 1: Butcher's Bloom Extract]
100 g of dried rhizome of Butcher's-broom (Ruscus aculeatus L) is crushed, 1 liter of 70 vol% ethanol aqueous solution is added thereto, and the mixture is extracted at 60 ° C. for 4 hours and then filtered. The solvent was distilled off to obtain about 1.3 g (solid content) of the extract.

[Preparation Example 2: Lupine extract]
Add 1 L of purified water and proteolytic enzyme to the crushed powder of 50 g of lupine albus seeds, hydrolyze, leave at low temperature for 2 to 3 days, filter, and leave the obtained filtrate for another 5 days. Aged and filtered again. The solvent was removed to obtain about 5.0 g (solid content) of the extract.

[Preparation Example 3: Tadeai Extract]
100 g of leaves and stems of Tadeai (Persicaria tinctoria) are crushed, 1 L of purified water is added, and the mixture is heated and extracted at 80 ° C. for about 5 hours and filtered. The solvent was distilled off to obtain 2.0 g (solid content) of the extract.

[Preparation Example 4: Olive Extract]
100 g of olive (Olea europaea Linne) leaves were pulverized, placed in 1 L of a 50 vol% ethanol aqueous solution, heated under reflux, and extracted. After filtering the insoluble material, the filtrate was concentrated under reduced pressure and the solvent was distilled off to obtain about 15 g (solid content) of the extract.

[Preparation Example 5: Guava extract]
To 100 g of dried guava (Psidium guajava) leaves, add 1 L of 25 vol% ethanol aqueous solution and perform reflux extraction for 2 hours. This is centrifuged, filtered under pressure, and collected using membrane separation to obtain 750 mL of the extract, concentrated under reduced pressure with an evaporator, the solvent is removed, and about 8.5 g of the extract (solid substance) is obtained. )Obtained.

[Preparation Example 6: Scutellaria baicalensis extract]
500 mL of ethanol is added to 100 g of dried roots of shredded Scutellaria baicalensis.L, heat-extracted at 80 ° C. for 2 hours with a reflux extractor, and filtered at hot temperature. The same extraction treatment was further carried out on the residue, and the obtained extracts were combined and concentrated under reduced pressure to remove the solvent to obtain about 9 g (solid content) of the extract.

[Preparation Example 7: Mugwort extract]
1 kg of leaves of Artemisia princeps Pampanini are chopped and extracted 3 times with 5 L of water-ethanol mixed solvent (water / ethanol = 10/90% by volume). The filtrates obtained by the three extractions were mixed, concentrated under reduced pressure, the solvent was removed, and about 20 g (solid content) of the extract was obtained.

[Preparation Example 8: Loquat Extract]
100 g of loquat (Eriobotrya japonica) leaves are chopped, soaked in 1 L of 50% 1,3-butylene glycol solution, extracted at room temperature for 3 days and nights, filtered, and the filtrate is concentrated under reduced pressure to concentrate the solvent. Distillation gave about 15 g (solid content) of the extract.

Manufacturing example 1
A phospholipid vesicle containing Butcher's Bloom extract was prepared as follows.
(Component) (% by mass)
1. 1. Residual amount of purified water 2. Butcher's Bloom Extract of Preparation Example 1 0.2
3. 3. Hydrogenated soybean phospholipid 10
4. Cholesterol 1
5. Glycerin 10
6.1,3-butylene glycol 10
(Production method)
A: Ingredients 1 and 2 were uniformly mixed at 80 ° C.
B: Ingredients 3 to 6 were uniformly mixed at 80 ° C.
C: A was added and mixed with B at 80 ° C.
After cooling D: C, it was treated with a microfluidizer to obtain a phospholipid vesicle containing Butcher's Bloom extract.

Examples 1 to 9 and Comparative Examples 1 to 4: Toners The toners having the compositions shown in Table 1 below are prepared by the following production methods, and after continuous use for one week, the "transparency improving effect" and "moisture retention ability improvement" of the skin are improved. Each item of "effect" and "effect of improving water absorption capacity" was evaluated by the evaluation method shown below, and the results are also shown in Table 1. In Example 4, the phospholipid vesicle containing the Butcher's Bloom extract from Production Example 1 was used. It was confirmed that the aloe vera extract and fennel extract used in the comparative example did not contain tannin.

(Production method)
A. Ingredients 1 to 6 are mixed and dissolved.
B. Ingredients 7-8 are mixed and dissolved.
C. Add A to B D. Ingredients 9 to 19 were added to C to obtain a lotion.

(Evaluation method)
For each item of "transparency improvement effect", "moisture retention ability improvement effect", and "moisture absorption ability improvement effect" of the skin, each sample of the example and the comparative example was continuously applied to a half face for one week on a panel of 10 people. I asked him to take a photo of the front of his face and took a photo using the mini studio VISIA-CR (trademark) (manufactured by CANFIELD). For photography, a color chart for image correction (Cassmatch, Bear Medeck Co., Ltd.) was attached to the forehead of the subject, and the photograph was taken using the function of Photoshop (Adobe Systems Incorporated) (registered trademark). An image for analysis was obtained by correcting each photographed image so that the white balance of Cassmatch was constant. Based on the comparison of images for analysis before and after continuous use, the "transparency improving effect" was evaluated by three cosmetics evaluation panel. The evaluation criteria are as follows. Scores were given according to the following 5-grade evaluation criteria, and the average value of the scores of all panels was judged according to the following 4-grade evaluation criteria.

(Score)
5 points: Especially excellent in transparency improvement effect 4 points: Excellent transparency improvement effect 3 points: Transparency improvement effect 2 points: Transparency improvement effect is slight 1 point: Transparency improvement effect is not available , From the average value, it is shown based on the following criteria.
(Evaluation criteria)
⊚: Average score is 4 points or more ○: Average score is 3 points or more and less than 4 points △: Average score is 2 points or more and less than 3 points ×: Average score is less than 2 points

"Effect of improving water retention"
In order to verify the effect of improving the water retention capacity by continuous application for one week in Examples and Comparative Examples, a water load test on the stratum corneum was performed. The stratum corneum water load test is generally used as a method for evaluating the water retention capacity of the stratum corneum, and the test method is described below. The subject thoroughly cleans the application site of the test product and acclimatizes for 15 minutes. Then, the water content of the stratum corneum was measured using SKION-200EX (manufactured by IBS), and the value was set to W0. Next, 50 μl of purified water was impregnated into a 1.0 cm ² piece of paper and applied to the test product application site for 60 seconds. After 60 seconds, the piece of paper was removed, and the water content in the stratum corneum after thoroughly wiping the water remaining on the skin was defined as Wmax. The water content of the stratum corneum was set to W30 to W120 every 30 seconds from 30 seconds to 120 seconds after the paper piece was removed. The measurement was performed at 3 sites, and the average value was taken as each value.
From the values obtained by the above method, the value calculated based on the following formula was used as the water retention capacity at each time point.
Moisture retention capacity = (W0 × W30 × W60 × W90 × W120) 5th root The higher the value of water retention capacity, the higher the ability to retain water and the better the state of the stratum corneum. The ratio of the water retention capacity after continuous application divided by the value before continuous application ([value after continuous application] / [value before continuous application]) was taken as the effect of improving the water retention capacity. The evaluation criteria are as follows.
(Evaluation criteria)
⊚: 1.4 or more ○: 1.2 or more and less than 1.4 △: 1.0 or more and less than 1.2 ×: less than 1.0

"Effect of improving water absorption capacity"
The effect of improving the water absorption capacity by continuous application for one week was calculated based on the following formula in Examples and Comparative Examples.
Moisture absorption capacity = Wmax-W0
It is shown that the higher the value of the water absorption capacity, the higher the ability to immediately absorb water into the inside and the better the state of the stratum corneum. The ratio of the water absorption capacity after continuous application divided by the value before continuous application ([value after continuous application] / [value before continuous application]) was taken as the effect of improving the water retention capacity. The evaluation criteria are as follows.
(Evaluation criteria)
⊚: 1.4 or more ○: 1.2 or more and less than 1.4 △: 1.0 or more and less than 1.2 ×: less than 1.0

As is clear from the results in Table 1, the lotions of Examples 1 to 11 are excellent in all of the "transparency improving effect", "moisture retention ability improving effect", and "water absorption ability improving effect" of the skin. there were. On the other hand, Comparative Example 1 containing no filaggrin production promoter had a low effect of improving transparency. Comparative Example 2 containing no tannin-containing plant extract was inferior in all of the effect of improving transparency, the effect of improving water retention ability, and the effect of improving water absorption ability. Further, Comparative Example 3 and Comparative Example 4 containing a plant extract containing no tannin had a low effect of improving transparency and water retention ability.

Further, in Example 4, "change in the amount of reflected light inside the skin" and "change in the presence distribution of desmoglein in the outermost layer of the stratum corneum" before and after continuous application for one month were measured with an instrument according to the following measurement method.
(Measuring method)
"Change in the amount of reflected light inside the skin"
The amount of reflected light inside the skin can be evaluated by irradiating the skin with light and measuring the reflected light from the inside of the skin with the Translucency Meter TLS850 (manufactured by Diastron). As shown in FIG. 1, the measuring device includes an LED illumination portion at one end having a width of 20 mm, and a plurality of light receiving portions extending from the LED illumination portion to the other end. A measuring device is applied to the cheek portion, the light emitted from the LED lighting portion is reflected inside the skin, and the reflected light is received by the light receiving portion. The farther from the LED illumination area, the less the reflected light inside the skin. If the internal structure of the skin is disturbed, light is scattered and absorbed inside the skin, so that the reflected light is reduced and the transparency is lowered. In addition, if the stratum corneum is rough, it is difficult for the irradiation light to enter the skin, and the transparency is lowered. The light to be irradiated is preferably blue light, for example, light having a wavelength of about 430 to 500 nm.

In this test, blue light (472 nm) was applied to the skin of the cheeks, and the total amount of blue reflected light from the inside of the skin was used as a parameter. As shown in FIG. 2, the amount of reflected light from the inside of the cheek portion (position of the light source) to which the synchrotron radiation is applied is taken on the Y-axis, and the distance from the portion to which the synchrotron radiation is applied is taken on the X-axis. Then, a curve is obtained in which the reflected light is attenuated as the distance increases. The total amount of reflected light is a value (area) obtained by integrating the curves.

FIG. 3 shows the results of measuring the amount of blue internal reflected light before and after the continuous application of Example 4. The results showed that the total amount of blue internally reflected light increased significantly after the continuous application as compared with the case before the continuous application. Therefore, it was clarified that the transparency was improved by the continuous application of Example 4.

"Changes in the distribution of desmoglein in the outermost layer of the stratum corneum"
The presence distribution of the stratum corneum cell adhesion factor can be evaluated by peeling and collecting the stratum corneum of the skin by tape stripping, and staining a plurality of collected stratum corneum samples by the fluorescent antibody method.

The skin condition can be evaluated by the presence distribution of desmoglein in the outermost layer of the stratum corneum. Transepidermal water loss (TEWL), weapon water content, and oil content are well-known parameters that indicate the condition of the stratum corneum or the condition of the skin. These parameters and the presence of desmoglein in the stratum corneum A correlation has been found with the state. In particular, it is known that there is a high correlation between TEWL and water content. Therefore, if desmoglein is stained and it is mainly present at the margin of the stratum corneum cells, it can be evaluated that the skin condition is good, and conversely, desmoglein is mainly present on the entire surface of the stratum corneum cells. If present in, it can be evaluated that the skin condition is not good.

(Sample preparation)
Cut a transparent double-sided tape (manufactured by Nichiban Co., Ltd.) into a size of 1.8 cm x 2.0 cm and attach it to a slide glass. Then, the adhesive surface of the slide glass was lightly pressed against the central part of the human cheek so that the entire surface of the tape adhered to the skin, and then peeled off from the skin to prepare a stratum corneum sample.

(Primary antibody reaction)
An appropriate amount of an anti-desmoglein 1 mouse IgG monoclonal antibody (manufactured by PROGEN) solution is applied to the tape surface of the slide glass sample, and the mixture is reacted at room temperature for 1 hour. Then, it is immersed in phosphate buffered saline (PBS) to wash away unreacted antibodies.

(Secondary antibody reaction)
An appropriate amount of a 200-fold PBS diluted solution of a fluorescently labeled anti-mouse IgG antibody (manufactured by Invitrogen) is applied to the tape surface of the slide glass sample reacted with the primary antibody, and the mixture is reacted at room temperature for 1 hour. Then, soak in PBS to wash away unreacted antibody.

(Enclosed)
Mount Quick Aqueous (SPISUPPLIES) was dropped on the surface of the slide glass sample reacted with the secondary antibody, and the sample was covered with a cover glass to prepare a sample for observation.

(observation)
The embedded slide glass sample was observed using an inverted fluorescence microscope manufactured by Olympus Corporation. This observation screen is shown in Fig. 4.

FIG. 4 shows the results of evaluating the distribution of desmoglein in the outermost layer of the horny layer before and after the continuous application of Example 4. Desmoglein was mainly present on the entire surface of the stratum corneum cells before the continuous application, whereas it was mainly present on the marginal portion of the stratum corneum cells after the continuous application. Therefore, it was clarified that the skin condition was improved by the continuous application of Example 4. Therefore, it is considered that the transparency, the water retention ability, and the water absorption ability are improved.

[Example 12: Solubilized lotion]
(Component) (% by mass)
1. 1. Polyoxyethylene (40 mol) hardened castor oil 0.5
2. 2. Polyoxyethylene (12 mol) dioleate 0.3
3. 3. Astaxanthin 0.05
4.1,3-butylene glycol 2.0
5. Glycerin 2.0
6. Ethanol 15.0
7. Tranexamic acid 2.0
8. Butcher's Bloom Extract of Preparation Example 1 0.1
9. Sodium lactate 0.2
10. Methyl paraoxybenzoate 0.1
11. Residual amount of purified water 12. Loquat extract of Preparation Example 8 0.00001
(Production method)
A. Ingredients 1 to 6 are mixed and dissolved.
B. Ingredients 7-12 are mixed and dissolved.
C. A was added to B to obtain a lotion.
The solubilized lotion of Example 12 was excellent in all of the "transparency improving effect", "water retention ability improving effect", and "water absorption ability improving effect" of the skin.

[Example 13: Non-woven fabric impregnated type pack charge]
(Component) (% by mass)
1. 1. PEG-20 cured castor oil 0.2
2. 2. Macadamia nut fatty acid phytosteryl 0.2
3. 3. Fragrance 0.2
4. Hydrogenated soybean lysophospholipid 0.05
5.1,3 Butylene glycol 10.0
6. Diglycerin 8.0
7. Tadeeye extract of Preparation Example 3 0.000005
8. Ethanol 3.0
9. Residual amount of purified water 10. Olive extract of Preparation Example 4 0.005
11. Hyaluronic acid 0.05
(Production method)
A: Ingredients 1 to 3 are dissolved and mixed at 80 ° C.
B: Ingredients 4 to 11 were heated to 70 ° C. and added to A for emulsification.
C: B was cooled and defoamed, and the non-woven fabric was impregnated with the undiluted solution to obtain a non-woven fabric impregnated type pack.
The non-woven fabric impregnated type pack material of Example 13 was excellent in all of the "transparency improving effect", "moisture retention ability improving effect", and "moisture absorption ability improving effect" of the skin.

[Example 14: Emulsion]
(Component) (% by mass)
1. 1. Hydrogenated lecithin 2
2. 2. Polyoxyethylene (20) Hardened castor oil 0.5
3. 3. Concentrated glycerin 10
4.1,3-butylene glycol 5
5. Phytosteryl oleate 1
6. Setostearyl alcohol 1.2
7. Ethyl oleate 3
8. Macadamia nut oil 2
9. Liquid paraffin 1
10. Olive oil 3
11. Remaining amount of purified water 12. Tadeai extract of Preparation Example 3 0.3
13. Scutellaria baicalensis extract of Preparation Example 6 0.00001
14. Carboxyvinyl polymer 1
15. Sodium hydroxide 0.35
16. Hydrolyzed hyaluronic acid (molecular weight 3000) 0.1
17. Polyvinyl alcohol 0.1
(Production method)
A: Ingredients 1 to 10 are uniformly mixed at room temperature and heated to 70 ° C.
B: Ingredient 11 is heated to 70 ° C., added to A and emulsified.
C: After cooling C to 40 ° C., components 12 to 17 were added to obtain a milky lotion. It was excellent in all of the "effects".

[Example 15: Cream]
(Component) (% by mass)
1. 1. Polyoxyethylene (40 mol) sorbitan monostearate 2.0
2. 2. Stearic acid 5.0
3. 3. Behenyl alcohol 0.5
4. Squalene 15.0
5. Beeswax 1.0
6. Phytosteryl oleate 5.0
7. Tocopherol 0.1
8. Methylparaben 0.1
9. Lupine extract of Preparation Example 2 0.0005
10. Mugwort extract of Preparation Example 7 0.001
11. Dipotassium glycyrrhizinate 0.01
12. Xanthan gum (2% aqueous solution) 7.0
13.1,3-butylene glycol 5.0
14. Residual amount of purified water 15. Fragrance 0.05
(Production method)
A. Ingredients 9 to 14 are heated and mixed and kept at 70 ° C.
B. Ingredients 1 to 8 are heated and mixed and kept at 70 ° C.
C. B is added to A, mixed and uniformly emulsified.
D. After cooling C, component 15 was added and mixed uniformly to obtain a cream.
The cream of Example 15 was excellent in all of the "transparency improving effect", "water retention ability improving effect", and "water absorption ability improving effect" of the skin.

[Example 16: Eye cream]
(Component) (% by mass)
1. 1. Glyceryl monostearate 3.5
2. 2. Polyethylene glycol monostearate (40 mol) 2.0
3. 3. Liquid paraffin 3.0
4. Vaseline 8.0
5. Glyceryl tri2-ethylhexanoate 5.0
6. Ascorbyl tetrahexyldecanoate 0.2
7. Setostearyl alcohol 2.0
8. Behenyl alcohol 2.0
9. Butcher's Bloom Extract of Preparation Example 1 0.002
10. Olive extract of Preparation Example 4 0.01
11. Ascorbic acid glucoside 0.5
12.1,3 Butylene Glycol 5.0
13. Xanthan gum 0.1
14. Remaining amount of purified water
15. Disodium edetate 0.05
16. Phenoxyethanol 0.1
17. Fragrance 0.003

(Production method)
A: Ingredients 1 to 8 are heated and dissolved at 70 ° C.
B: Ingredients 9 to 16 are heated and dissolved at 70 ° C., then added to A and emulsified.
After cooling C: B to room temperature, component 17 was added to obtain an oil-in-water eye cream.
The underwater oil type eye cream of Example 16 was an excellent underwater oil type eye cream in all of the "transparency improving effect", "moisture retention ability improving effect", and "water absorption ability improving effect" of the skin.

[Example 17: Ointment]
(Component) (% by mass)
1. 1. Stearyl alcohol 18.0
2. 2. Japan wax 20.0
3. 3. Polyoxyethylene (20 mol) monooleic acid ester 0.25
4. Glycerin monostearic acid ester 0.3
5. Vaseline 40.0
6. Residual amount of purified water 7. Glycerin 10.0
8. Adenosine-1-phosphate 0.5
9. Lupine extract of Preparation Example 2 0.04
10. Scutellaria baicalensis extract of Preparation Example 6 0.001
(Production method)
A. 1 to 5 are uniformly mixed at 70 ° C.
B. Heat 6-8 to 70 ° C.
C. Add B to A and emulsify.
D. C was cooled and 9-10 was added to obtain an ointment.
The ointment of Example 17 was excellent in all of the "transparency improving effect", "moisture retention ability improving effect", and "moisture absorption ability improving effect" of the skin.

[Example 18: Sunscreen]
(Component) (% by mass)
1. 1. Polyoxyethylene (25) Phytostannole (HLB14.5) 1.0
2. 2. Polyoxyethylene monooleate (20 mol) sorbitan 0.4
3. 3. Sorbitan sesquioleate 0.6
4. Behenyl alcohol 0.5
5.2 Glyceryl ethylhexanoate 2.0
6. 2-Ethylhexyl paramethoxycinnamate 10.0
7. Diethylaminohydroxybenzoyl hexyl benzoate 2.0
8. Dimethicodiethylbenzalmaronate 1.0
9. Tinosorb S (Note 1) 1.0
10. Octilt Azone 0.5
11. Dipropylene glycol 10.0
12. Triethanolamine 1.0
13. Residual amount of purified water 14. Glycerin 5.0
15.1,3 Butylene Glycol 5.0
16. Tinosorb M (Note 8) 1.0
17. Carboxyvinyl polymer 0.3
18. Purified water 5.0
19. Lupine extract of Preparation Example 2 0.02
20. Scutellaria baicalensis extract of Preparation Example 6 0.001
21. Citric acid 0.1
22. Disodium succinate 0.1
23. Ethanol 10.0
24. Phenoxyethanol 0.1
25. Fragrance 0.02
(Note 1) Made by BASF (manufacturing method)
A: Ingredients 1 to 10 are uniformly dissolved at 80 ° C.
B: Ingredients 11 to 20 are uniformly dissolved at 80 ° C.
C: Add A to B and emulsify.
D: C was stirred and cooled, and components 21 to 25 were added to obtain a sunscreen.
The sunscreen of Example 18 was excellent in all of the "transparency improving effect", "moisture retention ability improving effect", and "moisture absorption ability improving effect" of the skin.

[Example 19: Foundation]
(Component) (% by mass)
1. 1. Polyoxyethylene methylsiloxane / polyoxypropylene oleyl methylsiloxane / dimethylsiloxane copolymer (Note 2) 2.0
2. 2. PEG-3 Dimethicone (Note 3) 1.0
3. 3. Didimethylpolysiloxane 5.0
4. Dimethylpolysiloxane treated red iron oxide 1.0
5. Dimethylpolysiloxane treated iron oxide 1.5
6. Dimethylpolysiloxane treated black iron oxide 0.5
7. Dimethylpolysiloxane treated titanium oxide 10.0
8. Dimethylpolysiloxane treated talc 5.0
9. 2-Ethylhexyl paramethoxycinnamate 5.0
10. Stearyl glycyrrhetinate 0.5
11. Sorbitan sesquioleate 0.5
12. Residual amount of purified water 13.1,3-butylene glycol 15.0
14. Butcher's Bloom Extract of Preparation Example 1 0.005
15. Sodium citrate 0.1
16. Monosodium dihydrogen phosphate 0.1
17. Phenylbenzimidazole sulfonic acid 0.3
18. Triethanolamine 1.7
19. Methyl paraoxybenzoate 0.1
20. Fragrance 0.1
21. Guava extract of Preparation Example 4 0.001
(Note 2) KF-6026 (manufactured by Shin-Etsu Chemical Co., Ltd.)
(Note 3) KF-6015 (manufactured by Shin-Etsu Chemical Co., Ltd.)
(Production method)
A: Ingredients 1 to 3 are uniformly mixed.
B: Ingredients 4 to 11 are uniformly dispersed by a roller.
C: Add B to A and mix uniformly.
D: Components 12 to 21 are mixed and dissolved.
E: D was added to C and emulsified to obtain a foundation.
The foundation of Example 19 was excellent in all of the "transparency improving effect", "moisture retention ability improving effect", and "moisture absorption ability improving effect" of the skin.

Claims (7)

The following (A), (B), and (C)
(A) A plant extract selected from one or more of tannin, sanaeta, inutade, hartade, and miso soba, a phyllagrin production promoter (B) Asteraceae, Olive, Rosaceae, Eriobotrya, Guava , A cosmetic containing tannin-containing plant extract (C) phospholipid, which is selected from one or more species of the genus Eriobotrya in the family Asteraceae . The cosmetic according to claim 1, wherein the tannin-containing plant extract of (B) is a leaf extract. The cosmetic according to claim 1 or 2 , which is for improving the transparency of the skin. The cosmetic according to any one of claims 1 to 3 , which is for improving the water absorption capacity. The cosmetic according to any one of claims 1 to 4 , which is for improving the water retention ability. The cosmetic according to any one of claims 1 to 5 , which has an effect of promoting metabolism of a stratum corneum cell protein. The cosmetic according to claim 1, wherein (A) and (B) are vesicled using (C).

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