JP7058490B2 - Antibacterial composition that suppresses viruses, bacteria and fungi - Google Patents

Description

The present invention relates to ethanol formulations that inactivate viruses and kill bacteria and fungi.

Acute gastroenteritis due to norovirus and food poisoning due to Escherichia coli and Staphylococcus aureus are often problems. Some fungi also produce metabolites that are harmful to humans as they grow in food.
When these microorganisms and the harmful substances produced by them are taken up by the human body, the route is almost oral. Therefore, control of microorganisms in the cooking environment of food, that is, reduction of viruses, bacteria and fungi in the surface of cooking utensils and the air in the cooking room is important for food hygiene.

According to the Ministry of Health, Labor and Welfare, disinfectant ethanol and inverted soap are ineffective as chemicals used to inactivate norovirus, and sodium hypochlorite aqueous solution is effective. In addition, sodium hypochlorite is known to be effective against bacteria other than spore-forming bacteria.
However, on the other hand, sodium hypochlorite has a peculiar strong odor, is corrosive to the skin, decolorizes clothes, etc., reacts with acid to generate chlorine gas, and has physical characteristics that are difficult to use in a cooking environment. have.

Therefore, it is desirable that the drug used for controlling microorganisms around foods is a drug having higher safety, which is composed of the components contained in ordinary foods and drinks.
Ethanol is highly safe because it is a component contained in foods such as alcoholic beverages, but as mentioned above, it cannot be expected to have the effect of inactivating non-enveloped viruses such as norovirus by itself.

The present inventors have previously disclosed that calicivirus can be inactivated by using an aqueous solution containing ethanol, phosphoric acid, and glycerin fatty acid ester and having a pH of 2.5 to 5. (Patent Document 1). Furthermore, ethanol preparations containing ethanol, lactic acid, citric acid, and salts thereof in a predetermined ratio and having a pH of 3 to 4 have high antiviral activity, and have no white residue or liquid residue after spraying / coating. (Patent Document 2).

Japanese Patent No. 5542682 JP-A-2017-772232

An object of the present invention is to provide a drug capable of not only inactivating viruses but also killing bacteria and fungi. It is desirable that the raw material used is a component contained in general foods, which has little odor, corrosiveness, and irritation so that there is no problem even if it is used around foods.

The present inventors have found that ethanol preparations having a specific composition can inactivate or kill viruses, bacteria and fungi. That is, the present invention relates to the following (1) to (3).

(1) An antiviral, antibacterial and antifungal agent which is an aqueous solution in which the content and pH of each component satisfy the following (a) to (e).
(A) The ethanol concentration is 65 to 75% by weight, (b) the content of glycerin fatty acid ester is 0.03 to 0.15% by weight, and (c) the content of lactic acid or a salt thereof is 1. The content of (d) citric acid or a salt thereof, which is 0 to 1.8% by weight, is 0.2 to 0.5% by weight as citric acid, (e) pH is 3 to 4, and (2) in the above-mentioned drug. , The agent according to (1) above, wherein the content ratio (weight ratio) of lactic acid or a salt thereof and citric acid or a salt thereof is 3: 1 to 5: 1 as lactic acid and citric acid.
(3) A method for inactivating or sterilizing viruses, bacteria and fungi on the surface of an object, in which the agent according to (1) or (2) above is brought into contact with the object.

The agent (ethanol preparation) of the present invention efficiently inactivates or sterilizes a wide variety of microorganisms such as viruses, bacteria and fungi on the surface of an object by spraying, applying, mixing, etc. on the object. It is something that can be done. When this product is used, it has no odor that remains for a long time and has no adverse effect on the human body, so that it can be suitably used for disinfecting foods. Further, the pharmaceutical product of the present invention has almost no corrosiveness to metals, and if lactic acid and citric acid are used in an appropriate ratio, there is no white residue or liquid residue due to residual solid content after use, so that it is convenient as a spraying agent. Can be used for.

The ethanol preparation of the present invention has an ethanol concentration of 65 to 75% by weight. Within this range, safe and sufficient antiviral and antibacterial effects can be obtained. If it is less than 65% by weight, the antiviral and antibacterial effects are slightly weakened.

The ethanol preparation of the present invention contains 0.03 to 0.15% by weight of glycerin fatty acid ester. Specific examples of the glycerin fatty acid ester include monolauric acid monoglyceride and monocaprylic acid monoglyceride, which have an effect of enhancing antibacterial activity and antifungal activity.

The ethanol preparation of the present invention has a content of lactic acid or a salt thereof as lactic acid of 1.0 to 1.8% by weight. Moreover, the content of citric acid or a salt thereof is 0.2 to 0.5% by weight as citric acid. Within this range, a sufficient virus inactivating effect is exhibited.
Furthermore, if the content ratio (weight ratio) of lactic acid or its salt to citric acid or its salt is 3: 1 to 5: 1 as lactic acid and citric acid, it is difficult to leave white residue or liquid residue after spraying or coating. Therefore, it is still desirable.

The ethanol preparation of the present invention has a pH of 3 to 4. The lower the pH, the stronger the medicinal effect, but on the other hand, corrosion is more likely to occur when used on metals. Therefore, considering the balance, pH 3.4 to 3.7 is the most desirable.

Ethanol preparations in which the content and pH of the active ingredient satisfy the above conditions show sufficient antiviral, antibacterial and antifungal activities.
Further, a moisturizer such as polyglycerin fatty acid ester, hyaluronic acid and glycerin, a pigment, a fragrance and the like may be contained within a range that does not interfere with the medicinal effect.
Specific examples of the polyglycerin fatty acid ester include tetraglyceryl laurate, pentaglyceryl laurate, hexaglyceryl laurate, etc., which reduce the surface tension of the ethanol preparation and thus have the effect of increasing the contact area with the object. There is.

In the ethanol preparation of the present invention, for example, lactic acid or a salt thereof, citric acid or a salt thereof, and if necessary, a polyglycerin fatty acid ester or the like are dissolved in ethanol water so as to have the above concentrations, and the ethanol concentration and pH are adjusted to the above concentrations. It can be prepared by adjusting with an acid or an alkali so as to be, but the order of each step is not limited to this.

A method of inactivating or sterilizing a virus, bacterium and fungus with the ethanol preparation of the present invention is to bring the ethanol preparation and these microorganisms into contact for a sufficient time.
The required contact time varies depending on the type of microorganism to be controlled, its density and situation, but if it is desired to kill bacteria and fungi, contact for 30 seconds is usually sufficient. If you want to inactivate the virus, contact it for 1 minute or longer, preferably 5 minutes or longer.

Specifically, an ethanol preparation is sprayed, applied, mixed, or the like on an object suspected of having these microorganisms. The object may be immersed in an ethanol preparation. Examples of objects include tableware, cooking utensils, refrigerators, and cooks' clothes.
Examples of the present invention are given below, but the present invention is not limited to these examples.

Ethanol preparations having the compositions shown in Table 1 (Samples 1 to 3 as Examples and Samples 4 and 5 as Comparative Examples) were prepared. In addition, a pharmaceutical product containing 200 ppm of sodium hypochlorous acid was used as sample 6.

<Example 1> Feline calicivirus inactivating effect Using feline calicivirus F9 strain / feline kidney cells (CrFK), the virus infectivity titer was measured by the following method, and the virus activity was evaluated.

An equal amount of 100 μl of the sample solution and 100 μl of the virus solution were mixed and allowed to act at room temperature for 30 seconds or 60 seconds. As a control, a phosphate buffer solution was used instead of the sample solution. Immediately after the action, 100 μl of the mixture was added to 900 μl of MBM medium containing 2% fetal bovine serum and diluted 10-fold.
The 10-fold diluted mixture was further diluted 10-fold in MBM medium in 10 steps.
The cell proliferation medium (MEM medium containing 10% fetal bovine serum) of CRFK cells cultured on a 6-well plate for 3 days was removed with an aspirator, and 2-well cells were used for each step-diluted mixed solution. 100 μl was inoculated (N = 2).
After virus adsorption at 34 ° C. in a CO ₂ incubator for 1 hour, the cells were layered on an agar medium and cultured at 34 ° C. for 60 hours in a 5% CO ₂ incubator.
After completion of the culture, the number of plaques formed by 10% formalin fixation and methylene blue staining was counted, and the infectious titer was measured. The results are shown in Table 2.

As shown in Table 2, Samples 1 and 2 corresponding to the ethanol preparation of the present invention had a low infectious titer at an action time of 30 seconds and 60 seconds, and showed a high inactivating effect on feline calicivirus.

<Example 2> Bacterial effect against bacteria Escherichia coli, Staphylococcus aureus and Leuconostoc mesenteroides were used to obtain the bactericidal effect of the stock solution of Samples 1 to 5 and the 2- or 3-fold diluted solution, respectively. evaluated. Escherichia coli was cultured on a standard agar medium at 30 ° C. for 24 hours, yellow staphylococcus was cultured on a standard agar medium at 30 ° C. for 48 hours, and lactic acid bacteria were cultured on an MRS agar medium at 30 ° C. for 48 hours. The anaerobic cultures of the above were used respectively.

The bactericidal effect on Escherichia coli, Staphylococcus aureus and lactic acid bacteria was evaluated by the following method.
Add 0.05 ml each of the bacterial solution (concentration ¹⁰⁶ cfu / ml) to 3 ml of each sample, stir, and after 0, 10, and 30 seconds, immediately dilute the treatment solution with 100 times the amount of sterile water to stop the treatment. Then, 0.05 ml of the mixture was applied to each medium and then cultured, and the number of surviving bacteria was determined from the number of colonies generated. Results for Escherichia coli, Staphylococcus aureus and Lactic acid bacteria are shown in Table 3, Table 4 and Table 5, respectively.

As shown in Tables 3, 4 and 5, the alcohol preparation samples 1 to 3 of the present invention showed a remarkable bactericidal effect against any of Escherichia coli, Staphylococcus aureus and lactic acid bacteria. Further, since the bactericidal effect of the pharmaceutical product of the present invention is less likely to be lost even when diluted 2-fold or 3-fold, the bactericidal effect is less likely to be lost as compared with the comparative sample. It was suggested that an excellent bactericidal effect can be expected and that it is also effective for highly contaminated objects.

<Example 3> Bactericidal effect on fungi Using yeast-like fungi (Pichia anomala) that alters food, the bactericidal effect of the stock solution of Samples 1 to 5 and the 2-fold or 3-fold diluted solution was evaluated. The yeast-like fungus used was cultured in a potato dextrose agar medium at 25 ° C. for 48 hours.

The bactericidal effect on yeast-like fungi was evaluated by the following method.

0.05 ml of the bacterial solution (concentration ¹⁰⁶ cfu / ml) was added to 3 ml of each sample, and after stirring, the treatment solution was immediately diluted with 100 times the amount of sterile water after 0, 10 and 30 seconds to stop the treatment. After applying 0.05 ml thereof to each medium, the cells were cultured, and the number of surviving bacteria was determined from the number of colonies generated. The results are shown in Table 6.

As shown in Table 6, the ethanol-prepared samples 1 to 3 of the present invention also showed an excellent bactericidal effect against yeast-like fungi. Further, since the bactericidal effect of the pharmaceutical product of the present invention is less likely to be lost even when diluted 2-fold or 3-fold, the bactericidal effect is less likely to be lost as compared with the comparative sample. It was suggested that it can be expected to have a bactericidal effect and is also effective for highly contaminated objects.

From the above, it was shown that the ethanol preparation of the present invention has an inactivating and bactericidal effect on a wide variety of microorganisms such as viruses, bacteria and fungi.

The ethanol preparation of the present invention can be used for disinfection of any microorganism. Further, since there is no odor or solid matter remaining for a long time, it can be suitably used for disinfecting foods, specifically tableware, cooking machinery, refrigerators, clothes of cooks, and the like.

Claims (3)

An antiviral, antibacterial and antifungal agent which is an aqueous solution in which the content and pH of each component satisfy the following (a) to ( f ).
(A) The ethanol concentration is 65 to 75% by weight, (b) the content of monolauric acid monoglyceride is 0.03 to 0.15% by weight, and (c) the content of lactic acid or a salt thereof is 1. The content of (d) citric acid or a salt thereof, which is 0 to 1.8% by weight, is 0.2 to 0.5% by weight as citric acid, and (e) pH is 3 to 4.
(F) Contains lauric acid pentaglyceride The agent according to claim 1, wherein the content ratio (weight ratio) of lactic acid or a salt thereof to citric acid or a salt thereof is 3: 1 to 5: 1 as lactic acid and citric acid. A method for inactivating or sterilizing viruses, bacteria and fungi on the surface of an object, which brings the agent according to claim 1 or 2 into contact with the object.