JP6926378B2 - Microdust phagocytosis accelerator - Google Patents

Description

The present invention relates to a microdust phagocytosis accelerator that promotes the microdust phagocytosis of keratinocytes.

It is known that air pollution is caused by the floating and flight of various chemical substances and xenobiotics. Specifically, flue gas from automobiles, thermal power plants, incinerators, fireplaces, tobacco, etc., ejecta from volcanic eruptions, particulate matter (PM) derived from soil particles, dust, sulfur oxides (sulfur dioxide, etc.) ), Emissions such as nitrogen oxides (nitrogen dioxide, etc.), microscale particles such as asbestos.

In addition to adversely affecting the respiratory system by inhaling these micropollutants (microdust) through the mouth and nose, they are also taken into the body through the skin, which is the outermost layer of the living body and is constantly exposed to the outside world. There is concern that it may cause trouble or damage to the skin. For example, tobacco flue gas is known to reduce wrinkle formation and clarity in the skin through the production of nitrogen oxides or active oxygen species, and certain organic compounds cause skin inflammation. It has also been pointed out that there is a danger, and that fine particles of dust permeate the skin tissue while adsorbing aromatic hydrocarbons on the surface and affect the gene.

Therefore, attempts are being made to prevent microdust from entering the body through the skin and having an adverse effect. For example, it has been reported that by applying a composition having an α-gel structure to form a film on the skin surface, microdust is prevented from adhering to and permeating the skin (Patent Document 1).

Japanese Unexamined Patent Publication No. 2016-88866

However, the adhesion of microdust to the skin surface cannot be sufficiently suppressed only by the film made of the composition described in Patent Document 1. In addition to trying to prevent microdust from adhering to the surface of the skin, preventing the adhering microdust from penetrating into the skin is another approach to protecting the skin. Is important as.
In view of such a situation, it is an object of the present invention to provide a microdust phagocytosis accelerator.

We found that extracts of plants belonging to the family Broomrape (Orobanchaceae) and the genus Eyebright (Euphrasia), such as Euphrasia officinalis L., promote the microdust phagocytosis of keratinocytes of the epidermis. I found that I would let you. In addition, since keratinocytes phagocytose microdust and are discharged to the outside of the body while holding microdust by turnover, we came up with the idea that a microdust phagocytosis accelerator is suitable for a composition for improving skin damage. Completed the invention.

That is, one aspect of the present invention is a microdust phagocytosis promoter composed of an extract of a plant belonging to the genus Orobanchaceae and the genus Eyebright (Euphrasia). In this embodiment, the plant is preferably eyebright (Euphrasia officinalis L.).
Another aspect of the present invention is a composition for improving skin damage, which contains the microdust phagocytosis accelerator of the present invention.

The present invention provides a microdust phagocytosis accelerator. Further, a composition for improving skin damage containing a microdust phagocytosis accelerator is provided, and it is possible to recover damaged skin such as inflammation caused by irritation caused by microdust.

Confocal micrograph of normal human keratinocytes supplemented with fluorescent beads.

The microdust phagocytosis accelerator according to the first aspect of the present invention consists of an extract of a plant belonging to the genus Orobanchaceae and the genus Eyebright (Euphrasia). The plant belonging to the genus Eyebright is not particularly limited, but eyebright (Euphrasia officinalis L.) is preferable. Eyebright is the English name Eyebright, and the Japanese name Eyebright or eyebright (small rice grass).
It is a kind of herb known as.

As the plant extract in the present invention, an extract can be prepared using a plant sold as a native or grown eyebright, a raw material for Chinese herbal medicine, etc., and a plant extract from Koei Kogyo Co., Ltd., etc. is handled. Commercially available plant extracts sold by the company can also be purchased and used.

The site used for extraction may be a plant body, an above-ground part, a rhizome part, or a seed of a plant belonging to the genus Eyebright of the family Broomrape, such as eyebright, and these may be in a raw state or a dry state at the time of extraction. Further, it is preferable to crush or shred these in advance from the viewpoint of improving the extraction efficiency.
For extraction, add 1 to 30 parts by mass of the solvent to 1 part by mass of the plant body, aboveground part, rhizome part, and / or dried seed of a plant belonging to the genus Eyebright of the family Broomrape such as eyebright, and at room temperature. This can be done by immersing for several days at a temperature near the boiling point of the solvent for several hours. After the immersion, the mixture may be cooled to room temperature, and if desired, unnecessary substances may be removed, and then the solvent may be removed by concentration under reduced pressure or the like. After that, a desired extract can be obtained by fractionation generation by column chromatography or the like filled with silica gel or an ion exchange resin.
The solvent used for extraction is preferably a polar solvent, for example, water; alcohols such as ethanol, isopropyl alcohol and butanol; polyhydric alcohols such as 1,3-butanediol and polypropylene glycol; and ketones such as acetone and methyl ethyl ketone. ; And one or more selected from ethers such as diethyl ether and tetrahydrofuran are preferably mentioned.

The microdust phagocytosis accelerator of the present invention exerts an effect of promoting (enhancing) the phagocytic action of keratinocytes (keratinocytes) in the epidermis.
Here, microdust refers to all minute substances that have an adverse effect on the skin, and is not particularly limited, but for example, smoke exhaust from automobiles, thermal power plants, incinerators, fireplaces, tobacco, etc., ejecta from volcanic eruptions, soil particles. It refers to chemical substances and biological foreign substances such as particulate matter (PM), dust, sulfur oxides (sulfur dioxide, etc.), nitrogen oxides (nitrogen dioxide, etc.) and other exhaust gas, asbestos, etc. Further, it is usually a microscale particle, for example, a particle having a major axis of 20 μm or less.

Keratinocytes are generally known to phagocytose and take up melanosomes delivered from melanocytes, but like macrophages and Langerhans cells, they also exert a phagocytic effect on fine particles of 20 μm or less such as microdust. It is known to be taken up into cells (VB Morhenn et al., Dermatologic Surgery, vol. 28, no. 6, (2002) 484-490).
The microdust phagocytosis accelerator of the present invention can promote (enhance) the phagocytic action of keratinocytes on microdust as compared with the case where it is not applied, and can increase the amount of the uptake.
In the process of turnover, keratinocytes that have taken in microdust by phagocytosis usually rise toward the outer layer while holding the microdust, peel off, and are discharged to the outside of the body.

The application route of the microdust phagocytosis accelerator of the present invention is not particularly limited, such as transdermal, oral, nasal, and intravenous injection, but it is preferably taken transdermally.
The applied amount of the microdust phagocytosis accelerator of the present invention is not particularly limited, but for example, it is preferable to ingest 1 to 200 mg per day as the solid content of the extract of the plant in one or several divided doses. ..

The composition for improving skin damage according to the second aspect of the present invention contains the microdust phagocytosis accelerator of the present invention.

In this aspect, "skin damage" refers to any condition in which the skin is unhealthy, for example, a condition in which epidermal cells are stimulated to cause inflammation due to the release of inflammatory factors, and as a result, wrinkles, pigmentation, etc. The state that has occurred. Since microdust that has entered the epidermis generally causes a stimulus that promotes the release of inflammatory factors, the composition for improving skin damage of the present invention causes skin damage by promoting the microdust phagocytosis of keratinocytes. The cause of the problem can be removed and the damage can be repaired.

The composition for improving skin damage of the present invention preferably further contains burdock extract, vitamin E, and the like. Burdock extract and vitamin E are known to have the effect of regulating turnover, so they phagocytose and take in microdust, and keratinocytes go to the upper layer while holding it in the process of turnover and exfoliate. It can be efficiently discharged to the outside of the skin. That is, skin damage can be improved more efficiently.

The form of the composition for improving skin color of the present invention is not particularly limited, but is preferably an external composition for skin or an oral composition.

As the composition for external use on the skin, cosmetics, quasi-drugs, pharmaceuticals and the like can be preferably exemplified, and since they can be used on a daily basis, cosmetics and quasi-drugs are more preferable, and skin care cosmetics and makeup cosmetics are preferable. More preferred. The form thereof is not particularly limited to lotion, oil agent type, emulsifier type, cream, gel, spray and the like, and can be produced by a conventional method.

The composition of the present invention may contain any commonly used component. Such optional components include hydrocarbons such as squalane, vaseline and microcrystalin wax, esters such as jojoba oil, carnauba wax and octyldodecyl oleate, triglycerides such as olive oil, beef fat and coconut oil, stearic acid and olein. Acids, fatty acids such as retinoic acid, higher alcohols such as oleyl alcohol, stearyl alcohol, octyldodecanol, anionic surfactants such as sulfosuccinate and sodium polyoxyethylene alkyl sulfate, and amphoteric surfactants such as alkyl betaine salts. , Cationic surfactants such as dialkylammonium salts, sorbitan fatty acid esters, fatty acid monoglycerides, these polyoxyethylene adducts, polyoxyethylene alkyl ethers, nonionic surfactants such as polyoxyethylene fatty acid esters, polyethylene glycol, Polyhydric alcohols such as glycerin and 1,3-butanediol, sugar alcohols, thickening / gelling agents, antioxidants, UV protective agents such as UV absorbers and UV scatterers, coloring agents, preservatives, pH Suitable examples include modifiers, powders, various active ingredients such as whitening ingredients and anti-wrinkle ingredients, and various plant extracts.

The amount of the microdust phagocytosis accelerator contained in these external preparations for skin is not particularly limited, but is 0. It is preferably 001 to 5% by mass, more preferably 0.01 to 0.5% by mass.

As the oral composition, foods and drinks, supplements (dietary supplements), quasi-drugs, pharmaceuticals and the like can be preferably exemplified, and since they can be used on a daily basis, foods and drinks and quasi-drugs are more preferable. In addition to general foods such as confectionery, breads, and noodles, the form can be various dosage forms such as drink preparations, capsules, tablets, vulcanizers, powders, and liquids. Can be manufactured.

The oral composition may contain any physiologically acceptable ingredient. Such optional ingredients include, in the case of food and drink, seasoning ingredients such as salt, sugar, monosodium glutamate, sodium inosinate, and vinegar, coloring ingredients, flavoring ingredients such as flavors, thickeners, emulsifying / dispersing agents, preservatives, etc. Stabilizers, various vitamins and the like can be preferably exemplified. In the case of supplements, non-pharmaceutical products, pharmaceuticals, excipients such as crystalline cellulose and lactose, binders such as gum arabic and hydroxypropyl cellulose, disintegrants such as croscarmellose sodium and starch, magnesium stearate, etc. Examples thereof are preferably used as a lubricant, a flavoring / smelling agent, a coloring agent, various vitamins, a pH adjusting agent, and the like.

The amount of the microdust phagocytosis accelerator contained in these oral compositions is not particularly limited, but is 0. It is preferably 01 to 10% by mass, more preferably 0.1 to 5% by mass.

Hereinafter, the present invention will be described in more detail with reference to Examples and the like, but the present invention is not limited to the following Examples as long as the gist thereof is not exceeded.

<Example 1: Evaluation of microdust phagocytosis promoting action>
The microdust phagocytosis promoting effect of the eyebright extract was evaluated by the following procedure.
Normal human keratinocytes (frozen NHEK neonatal origin, Kurabo Industries product) were ^{sown in 4-well chamber plates so as to be 7.0 × 10 4} pieces / well, respectively, and HuMedia-KG2 Kurabo Industries growth medium (Kurabo Industries product). Was cultured in a 5% CO _{2 environment at 37 ° C.} Twenty-four hours after sowing, fluorescent microbeads with a diameter of 0.5 μm (TetraSpeck ^TM Microspheres,
0.5 μm, fluorescent, manufactured by Thermo Fisher) 5 × 10 ⁶ pieces / well, and eyebright extract (product of Koei Kogyo Co., Ltd.) were added to a final concentration of 0% by mass or 0.3% by mass, and 18 Incubated for hours. The cells were then washed with PBS (−) to remove microbeads in the medium, and the cells were immobilized with 4% paraformaldehyde and encapsulated. Fluorescence was observed for each with a confocal microscope.
As shown in the results shown in FIG. 1, it was confirmed that the keratinocytes to which the eyebright extract was added promoted the phagocytic action of microdust.

The present invention provides a microdust phagocytosis accelerator. Further, a composition for improving skin damage containing a microdust phagocytosis accelerator is provided, and it is possible to recover damaged skin such as inflammation caused by irritation caused by microdust. The composition is industrially useful because it can be suitably used as an external preparation for skin such as skin care cosmetics and make-up cosmetics.

Claims (2)

Microdust phagocytosis accelerator consisting of extracts of plants belonging to the genus Orobanchaceae and the genus Eyebright (however, it is used for the mode used for removing active oxygen and for improving skin damage not caused by microdust. Except for aspects) . The microdust phagocytosis accelerator according to claim 1, wherein the plant is eyebright (Euphrasia officinalis L.) (however, for the embodiment used for removing active oxygen and for improving skin damage not caused by microdust. Except for the mode used) .

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