JP6142197B2 - Method for producing natto enriched with ornithine - Google Patents

Description

  The present invention belongs to a technical field related to a method for producing natto enriched in ornithine.

Ornithine consisting of the molecular formula C ₅ H ₁₂ N ₂ O ₂ exists in the body as a free amino acid that does not constitute a protein, and has various physiological functions (liver function improving action, growth hormone secretion promoting action, immune function improving action, It is said to have muscle strengthening action, basal metabolism improvement action, and fatigue improvement action. However, since ornithine is not so much contained in general foods, it is difficult to actively take ornithine in a normal diet.

  Currently, various drugs and supplements with ornithine are provided for the purpose of active intake of ornithine. However, these drugs and supplements have problems in terms of production cost and efficiency of ornithine absorption into the body. For this reason, there is a demand for the development of a technology that enables the active intake of ornithine by enriching ornithine contained in general foods.

  Conventionally, as a technique for enriching ornithine contained in general foods, for example, those described in Patent Documents 1 to 6 are known.

  Patent Documents 1 to 6 describe techniques for enriching ornithine contained in general foods by increasing the amount of ornithine produced by involving lactic acid bacteria in the degradation of arginine.

Japanese Patent No. 4402633 No. 2007-29719 JP 2008-17703 A JP 2009-112205 A JP 2011-36161 A JP 2010-99862 A

  According to the techniques according to Patent Documents 1 to 6, it is suggested that conversion from arginine to ornithine can be promoted also by other fungi other than lactic acid bacteria.

  The present inventor thinks that natto bacteria can promote the conversion of arginine to ornithine, and confirms that ornithine is produced from arginine by culturing various natto bacteria in a medium containing arginine solution. did. Based on this confirmation, a technology that enables the active intake of ornithine by enriching the ornithine contained in natto, which is a common food, high in nutritional value and healthy food, and frequently eaten. It was decided to meet the above-mentioned request for development of

  The production of natto already exists as a mature technology. Therefore, when enriching ornithine contained in natto, it is desirable not to change the normal manufacturing process.

  This invention is made | formed in view of such a point, and makes it a subject to provide the manufacturing method of natto which can enrich the ornithine contained, without changing a normal manufacturing process.

  In order to solve the above-described problems, the method for producing natto according to the present invention employs means described in each claim.

  That is, claim 1 is characterized in that arginine is added after the middle of the logarithmic phase of the growth curve of Bacillus natto in the fermentation process in which soybean is inoculated with fermented soybean and fermented.

  This measure avoids the utilization of arginine and ornithine during the growth of natto, and the logarithm of the growth curve of natto as the time when natto produces an enzyme and effectively promotes the conversion of arginine to ornithine. After the middle of the period, the subsequent process is selected so that it does not affect the manufacturing process other than the addition period of arginine.

  Further, according to claim 2, in the method for producing natto according to claim 1, the amount of arginine added is 600 mg or less per 100 g of soybean.

  By this means, by restricting the amount of arginine added, it is possible to prevent the occurrence of a savory taste that makes it more intense.

  The method for producing natto according to the present invention avoids assimilation of arginine and ornithine when natto grows, and natto as a time when natto produces an enzyme to effectively promote the conversion of arginine to ornithine. After the middle of the logarithmic phase of the growth curve of the fungus is selected, so that it does not affect the manufacturing process other than the time of addition of arginine, it is contained without changing the normal manufacturing process This has the effect of enriching ornithine.

  Furthermore, as claimed in claim 2, by limiting the amount of arginine added, it is possible to prevent the occurrence of a gummy taste that becomes strong, so even if enriched ornithine is contained, it is used as natto. There is an effect that the taste can be maintained.

It is process drawing (including the graph of the growth curve of Bacillus natto) of the form for enforcing the manufacturing method of Natto concerning the present invention. It is a table | surface which shows the relationship between the addition amount of arginine of FIG. 1, and taste. It is a table | surface of the experimental result for specifying the addition time of arginine in FIG. 4 is a table showing the relationship between arginine, ornithine and urea in FIG. 3.

  Hereinafter, the form for carrying out the manufacturing method of natto concerning the present invention is explained based on a drawing.

  As shown in Fig. 1, this form uses the flow of "immersion", "steaming", "inoculation", "filling", "fermentation", and "refrigeration", which are the usual manufacturing processes for natto, as they are. To be implemented.

  “Immersion” is what makes soybeans absorb water. The soaking time is adjusted according to the quality, size and temperature of soybean. Prior to “immersion”, sorting such as removal of defective soybeans and foreign matters and washing of soybeans are performed.

  “Steamed” is to steam soybeans in a pressure cooker or the like. The cooking time is adjusted according to the soybean state.

  “Inoculation” is a method in which natto a is added (inoculated) to soybeans. The cultured Bacillus natto a is diluted to an appropriate concentration and sprayed on soybeans.

  “Filling” is to fill the inoculated soybeans in a container. Weighing is performed when soybeans are loaded into a container.

  “Fermentation” is to ferment soybeans inoculated in a container in a fermentation room. Temperature and humidity are adjusted in the fermentation room.

  “Refrigeration” cools fermented soybeans. The cooled soybean is aged by reducing the activity of Bacillus natto.

  In this form, arginine is added to soybeans after the middle of the logarithmic phase of the growth curve of Bacillus natto a in the “fermentation” process (the time when the Bacillus natto vegetative cells start vigorous division and the number of bacteria exponentially increases) Add to. About the addition time of arginine, it can choose until the product completion as natto.

  Arginine is added to soybean as arginine solution b. As this adding means, dripping, spraying and the like are appropriately selected.

  According to this form, it was confirmed that the content of ornithine in the finished natto was increased from several times to a dozen times as compared with the case where arginine was not added (about 20 mg / 100 g at most). . For this reason, natto, which is a common food and has a high nutritional value and is a healthy food, is frequently eaten, so that ornithine can be actively consumed. In addition, it can apply easily to the present natto manufacturing site which can be implemented using the flow of the normal manufacturing process of natto as it is.

  As for Bacillus natto a, it is known that a large amount of extracellular enzymes are produced when the logarithmic phase of the growth curve is reached. This extracellular enzyme is thought to be involved in the conversion of arginine to ornithine. However, if arginine is added after the middle of the logarithmic phase of the growth curve of Bacillus natto a, the content of ornithine in the finished natto is considerably increased.

  In addition, about the logarithmic phase of the growth curve of Bacillus natto a in the normal manufacturing process of natto described above, it is approximate to the time course and the growth curve by accumulation of experimental data without examining the number of Bacillus natto a. It can be grasped as a change in product temperature that becomes a curve.

  As for the amount of arginine added, the ornithine content in the finished natto is increased in proportion to the amount added. However, it has been confirmed that if the content of ornithine is increased too much, the taste of natto is lowered. The table | surface shown in FIG. 2 has shown the evaluation as a result of the sensory test about the relationship with the taste of natto of the addition amount of arginine per 100g of soybeans. As apparent from FIG. 2, when the amount of arginine added per 100 g of soybean greatly exceeds 600 mg, the gustatory taste that makes the vine becomes strong and the taste familiar to natto cannot be maintained.

  Moreover, when acid was added to the alkaline arginine solution b and the pH was adjusted and added, it was confirmed that the conversion from arginine to ornithine was reduced when it became acidic. Therefore, it is considered that the pH adjustment of the arginine solution b to be added is not so necessary.

  As described above, arginine can be added in a form other than the aqueous solution in addition to the illustrated form.

  The experiment which became the basis of the form for implementing the manufacturing method of the natto concerning the above-mentioned this invention is demonstrated.

  For the addition of arginine, 5 ml of a 50 mg / ml arginine solution adjusted to pH 7 was added to 50 g (1 pack) of soybeans. That is, 500 mg of arginine was added per 100 g of soybean.

  The fermentation time in the “fermentation” step was set to 19 hours. In the initial 0 to 18 hours, the temperature of the fermentation chamber was 40 ° C. During the remaining 1 hour, the temperature of the fermentation chamber was gradually lowered to 20 ° C.

  The “refrigeration” process was performed by maintaining 5 ° C. for 24 hours. However, about addition (11) mentioned later, after addition of arginine solution b, 5 degreeC was maintained for 24 hours.

According to this experiment, as shown in FIG. 3, when the addition time was set from addition (1), which is the start of fermentation, to addition (11), which is 44 hours after the start of fermentation (fermentation is complete), natto It has been clarified that the content of ornithine in the finished natto is significantly increased from the addition (4), which is 9 hours after the start of fermentation after the middle of the logarithmic phase of the growth curve of the fungus a. Incidentally, the addition (3) that does not reach the middle of the logarithmic phase of the growth curve of Bacillus natto a is 68.6 mg / 100 g, whereas the addition (4) is 110.1 mg / 100 g. And
At the time of addition after the end of the logarithmic phase of the growth curve of Bacillus natto a (11 hours after the start of fermentation, around 12 hours of addition (6), (7)), the ornithine content in the finished natto is 143.9. It has been shown that the dose is increased more than mg / 100g. Moreover, this tendency to increase the amount of ornithine continues until the addition (11) at which the fermentation is complete.

  FIG. 4 shows the residual rate of arginine, the conversion rate of ornithine, and the conversion rate of urea for the addition timings of addition (1) to (11). The reason why each ratio is labeled “apparent” is because the influence of arginine due to the degradation of soybean protein was taken into account. In addition, the reason why each ratio is set to “molar ratio” instead of “weight ratio” is to facilitate understanding of the conversion ratio from the reaction from “arginine” + “water” to “ornithine” + “urea”. .

  From FIG. 4, it can be considered that during the induction period and the logarithmic period of the growth curve of Bacillus natto a, Bacillus natto a assimilates arginine and ornithine for growth.

  That is, Bacillus natto a is in a spore state when inoculated, and vegetative cells that germinate and grow when the growth curve reaches the logarithmic phase undergo cell division. At this time, arginine and ornithine are suitable nutrients for the growth of Bacillus natto a.

  The method for producing natto according to the present invention can be widely implemented from a small-scale natto production factory by hand to a large-scale automated natto production factory.

a natto bacteria b arginine solution

Claims (2)

  Manufacture of ornithine-enriched natto, characterized by adding arginine after the middle of the logarithmic phase of the growth curve of Bacillus natto in the fermentation process in which soybean is inoculated with fermented natto. Method.   The method for producing natto according to claim 1, wherein the amount of arginine added is 600 mg or less per 100 g of soybean.

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