JP5909200B2 - Food and drink containing lactic acid bacteria and method for producing the same - Google Patents
Food and drink containing lactic acid bacteria and method for producing the same Download PDFInfo
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- JP5909200B2 JP5909200B2 JP2013015982A JP2013015982A JP5909200B2 JP 5909200 B2 JP5909200 B2 JP 5909200B2 JP 2013015982 A JP2013015982 A JP 2013015982A JP 2013015982 A JP2013015982 A JP 2013015982A JP 5909200 B2 JP5909200 B2 JP 5909200B2
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- lactic acid
- acid bacteria
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- food
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Description
本発明は経時後であっても、食感が良好であり、ホエー浮が抑制された乳酸菌含有飲食品に関するものである。 The present invention relates to a lactic acid bacteria-containing food or drink that has a good texture even after the passage of time and has suppressed whey float.
乳酸菌を含有する飲食品は、従来より発酵乳、乳酸菌飲料等、多種多様な形態の製品が流通している。我国は、高齢化社会の進行等に伴って、食を通じた健康確保への関心が高まっており、その中でも、所謂善玉菌と称される細菌による体内環境の改善（プロバイオティクス）についての関心は高く、プロバイオティクスの代表的な細菌として、特に乳酸菌が注目されている。 As foods and drinks containing lactic acid bacteria, products in various forms such as fermented milk and lactic acid bacteria drinks have been distributed. In Japan, with the progress of an aging society, interest in securing health through food is increasing, and among them, interest in improving the internal environment (probiotics) by bacteria called so-called good bacteria. As a typical probiotic bacterium, lactic acid bacteria are particularly attracting attention.
乳酸菌は、生存状態で腸に届いた場合には、腸内環境を整える作用を有する。具体的には、食物の消化吸収を促進すると共に、大腸菌等の所謂悪玉菌を減らす等、直接的な生理活性作用を発揮する。しかしながら、乳酸菌は、体内に摂取されると胃酸が発生している胃などの過酷な環境を通過するため、生存状態で腸に到達するのが困難な状況にある。このことから、一般的に流通している乳酸菌含有飲食品に含まれる乳酸菌は、製品の製造過程において予め加熱殺菌された殺菌体の状態で含有されている製品もある。 Lactic acid bacteria have the effect of adjusting the intestinal environment when they reach the intestine in a living state. Specifically, it promotes digestion and absorption of food and exerts a direct physiological activity such as reducing so-called bad bacteria such as E. coli. However, since lactic acid bacteria pass through a harsh environment such as the stomach where stomach acid is generated when ingested in the body, it is difficult to reach the intestine in a living state. For this reason, there are products in which lactic acid bacteria contained in lactic acid bacteria-containing foods and drinks that are generally distributed are contained in the form of a sterilized body that has been heat-sterilized in advance during the production process of the products.
一方で、近年、殺菌体の乳酸菌であっても、人体に有用な生理活性作用を奏するという報告もなされている。例えば、乳発酵させた際に生成される乳酸が、腸内善玉菌を増加させる作用がある他、殺菌体自体が消化吸収の促進する作用があることが明らかにされている。更に近年においては、乳酸菌の菌体表面の特定の蛋白が、腸中の免疫活性及びアレルギー抑制等に対しても効果を発揮するとの報告もある。例えば、特許文献１（特開２００７−１０５０１７号公報）には、乳酸菌の殺菌体を添加することにより、中性脂肪やコレステロールの低下効果などの優れた薬理作用を有する乳酸菌発酵乳が開示されている。 On the other hand, in recent years, it has been reported that even a sterilized lactic acid bacterium exhibits a physiologically active action useful for the human body. For example, it has been clarified that lactic acid produced when milk is fermented has an effect of increasing good bacteria in the intestine and that the sterilized body itself has an action of promoting digestion and absorption. Furthermore, in recent years, it has been reported that specific proteins on the surface of lactic acid bacteria have an effect on intestinal immune activity and allergy suppression. For example, Patent Document 1 (Japanese Patent Application Laid-Open No. 2007-105017) discloses lactic acid bacteria fermented milk having excellent pharmacological effects such as neutral fat and cholesterol lowering effects by adding a sterilized lactic acid bacterium. Yes.
以上のように、乳酸菌には生菌・殺菌体に関わらず注目すべき様々な効果が期待されており、乳酸菌を手軽に摂取できる、乳酸菌含有飲食品への消費者ニーズも高まっている。しかしながら、このような乳酸菌含有飲食品は、製造後の流通及び販売などの場面において、時間経過と共に食感がぼそぼそし始め、ヨーグルト表面に水分が浮き出る所謂ホエー浮が目立つなど、食感及び性状面でマイナスの変化を生じる問題があった。 As described above, lactic acid bacteria are expected to have various remarkable effects regardless of whether they are viable or sterilized, and consumer needs for foods and drinks containing lactic acid bacteria that allow easy intake of lactic acid bacteria are also increasing. However, such lactic acid bacteria-containing foods and drinks, such as distribution and sales after production, the texture begins to become voluminous over time, the so-called whey floating out of the surface of the yogurt is conspicuous and the texture and properties There was a problem that caused a negative change.
この問題を解決すべく、乳酸菌含有飲食品の食感及び性状面を向上させる種々の試みがなされている。例えば、特許文献２（特開２００３−２７４８５２号公報）は、イネ科植物から抽出されたβ−グルカンを配合することにより、ヨーグルトの食感を維持したヨーグルト類が記載されている。また、特許文献３（再表２００６−０７５７７２号公報）には、原料乳にタンパク質脱アミド酵素を作用させることにより、食感がなめらかな乳製品を提供している。特許文献４（再表２００５−１１０１０８号公報）には、トランスグルタミナーゼと、ホエー蛋白質の変性度が６０〜８８％となる条件でホエー蛋白質を加熱処理して得られる乳蛋白質処理物を用いることにより、食感が良好で離水が防止されたヨーグルトを提供している。しかしながら、これらの方法は、イネ科植物からのβ−グルカン抽出処理や酵素処理を要するため、操作が煩雑であった。 In order to solve this problem, various attempts have been made to improve the texture and properties of foods and drinks containing lactic acid bacteria. For example, Patent Document 2 (Japanese Patent Laid-Open No. 2003-274852) describes yogurts that maintain the texture of yogurt by blending β-glucan extracted from a grass family plant. Further, Patent Document 3 (Re-Table 2006-075752) provides a dairy product with a smooth texture by allowing protein deamidase to act on raw milk. Patent Document 4 (Republished 2005-110108) uses transglutaminase and a milk protein processed product obtained by heat-treating whey protein under the condition that the degree of denaturation of whey protein is 60 to 88%. It provides yogurt with good texture and water separation prevention. However, since these methods require β-glucan extraction treatment and enzyme treatment from gramineous plants, the operation is complicated.
本発明は、経時後であっても食感が良好であり、ホエー浮が抑制された乳酸菌含有飲食品を提供することを目的とする。 An object of the present invention is to provide a food or drink containing lactic acid bacteria that has a good texture even after lapse of time and has suppressed whey float.
本発明は、以下に関するものである。
１．成分Ａ及び／又はＢと、成分Ｃ及びＤとを含み、（Ａ＋Ｂ）／（Ａ＋Ｂ＋Ｃ）が０．００１以上であり、かつ（Ａ＋Ｂ）／Ｄ（個／ｇ）が１．５Ｅ＋６〜１．０Ｅ＋１２であることを特徴とする食感とホエー浮が改善された乳酸菌含有飲食品。
成分Ａ：エンテロコッカス・フェカリス菌（生菌）（個／ｇ）
成分Ｂ：エンテロコッカス・フェカリス菌（殺菌体）（個／ｇ）
成分Ｃ：エンテロコッカス・フェカリス菌以外の乳酸菌（生菌）（個／ｇ）
成分Ｄ：無脂乳固形分（ｇ／ｇ）
２．前記成分Ｃが、ラクトバチルス属、ラクトコッカス属、ペディオコッカス属、ロイコノストック属、ビフィドバクテリウム属、ストレプトコッカス属及びエンテロコッカス属に属する乳酸菌から選択される乳酸菌を含むことを特徴とする１に記載の乳酸菌含有飲食品。
３．前記成分Ａ及び／又はＢが、１．０Ｅ＋６〜１．０Ｅ＋１１（個／ｇ）であることを特徴とする１又は２に記載の乳酸菌含有飲食品。
４．前記成分Ａ、Ｃ及びＤを含み、Ａ／（Ａ＋Ｃ）が０．００１以上であり、かつＡ／Ｄが１．５Ｅ＋６〜１．０Ｅ＋１２であることを特徴とする１〜３のいずれかに記載の乳酸菌含有飲食品。
５．成分Ａ及び／又はＢと、成分Ｃ及びＤとを含有させ、（Ａ＋Ｂ）／（Ａ＋Ｂ＋Ｃ）が０．００１以上の比率となるように調整し、かつ（Ａ＋Ｂ）／Ｄ（個／ｇ）が１．５Ｅ＋６〜１．０Ｅ＋１２となるように調整することを特徴とする食感とホエー浮が改善された乳酸菌含有飲食品の製造方法。
成分Ａ：エンテロコッカス・フェカリス菌（生菌）（個／ｇ）
成分Ｂ：エンテロコッカス・フェカリス菌（殺菌体）（個／ｇ）
成分Ｃ：エンテロコッカス・フェカリス菌以外の乳酸菌（生菌）（個／ｇ）
成分Ｄ：無脂乳固形分（ｇ／ｇ）
６．前記成分Ｃが、ラクトバチルス属、ラクトコッカス属、ペディオコッカス属、ロイコノストック属、ビフィドバクテリウム属、ストレプトコッカス属及びエンテロコッカス属に属する乳酸菌から選択される乳酸菌を含むことを特徴とする５に記載の乳酸菌含有飲食品の製造方法。
７．前記成分Ａ及び／又はＢが、１．０Ｅ＋６〜１．０Ｅ＋１１（個／ｇ）となるように調整することを特徴とする５又は６に記載の乳酸菌含有飲食品の製造方法。
８．前記成分Ａ、Ｃ及びＤを含有させ、Ａ／（Ａ＋Ｃ）が０．００１以上の比率となるように調整し、かつＡ／Ｄ（個／ｇ）が１．５Ｅ＋６〜１．０Ｅ＋１２となるように調整することを特徴とする５〜７のいずれかに記載の乳酸菌含有飲食品の製造方法。
９．成分Ａ及び／又はＢと、成分Ｃ及びＤとを含有させ、（Ａ＋Ｂ）／（Ａ＋Ｂ＋Ｃ）が０．００１以上の比率となるように調整し、かつ（Ａ＋Ｂ）／Ｄ（個／ｇ）が１．５Ｅ＋６〜１．０Ｅ＋１２となるように調整することを特徴とする乳酸菌含有飲食品の性状安定化方法。
成分Ａ：エンテロコッカス・フェカリス菌（生菌）（個／ｇ）
成分Ｂ：エンテロコッカス・フェカリス菌（殺菌体）（個／ｇ）
成分Ｃ：エンテロコッカス・フェカリス菌以外の乳酸菌（生菌）（個／ｇ）
成分Ｄ：無脂乳固形分（ｇ／ｇ）
１０．前記成分Ｃが、ラクトバチルス属、ラクトコッカス属、ペディオコッカス属、ロイコノストック属、ビフィドバクテリウム属、ストレプトコッカス属及びエンテロコッカス属に属する乳酸菌から選択される乳酸菌を含むことを特徴とする９に記載の乳酸菌含有飲食品の性状安定化方法。
１１．前記成分Ａ及び／又はＢが、１．０Ｅ＋６〜１．０Ｅ＋１１（個／ｇ）となるように調整することを特徴とする９又は１０に記載の乳酸菌含有飲食品の性状安定化方法。
１２．前記成分Ａ、Ｃ及びＤを含有させ、Ａ／（Ａ＋Ｃ）が０．００１以上の比率となるように調整し、かつＡ／Ｄ（個／ｇ）が１．５Ｅ＋６〜１．０Ｅ＋１２となるように調整することを特徴とする９〜１１のいずれかに記載の乳酸菌含有飲食品の性状安定化方法。
１３．成分Ａ及び／又はＢと、成分Ｃ及びＤとを含有させ、（Ａ＋Ｂ）／（Ａ＋Ｂ＋Ｃ）が０．００１以上の比率となるように調整し、かつ（Ａ＋Ｂ）／Ｄ（個／ｇ）が１．５Ｅ＋６〜１．０Ｅ＋１２となるように調整することを特徴とする乳酸菌含有飲食品の食感改善方法。
成分Ａ：エンテロコッカス・フェカリス菌（生菌）（個／ｇ）
成分Ｂ：エンテロコッカス・フェカリス菌（殺菌体）（個／ｇ）
成分Ｃ：エンテロコッカス・フェカリス菌以外の乳酸菌（生菌）（個／ｇ）
成分Ｄ：無脂乳固形分（ｇ／ｇ）
１４．前記成分Ｃが、ラクトバチルス属、ラクトコッカス属、ペディオコッカス属、ロイコノストック属、ビフィドバクテリウム属、ストレプトコッカス属及びエンテロコッカス属に属する乳酸菌から選択される乳酸菌を含むことを特徴とする１３に記載の乳酸菌含有飲食品の食感改善方法。
１５．前記成分Ａ及び／又はＢを、１．０Ｅ＋６〜１．０Ｅ＋１１（個／ｇ）となるように調整することを特徴とする１３又は１４に記載の乳酸菌含有飲食品の食感改善方法。
１６．前記成分Ａ及びＣを、Ａ／（Ａ＋Ｃ）が０．００１以上の比率となるように含有させ、かつＡ／Ｄ（個／ｇ）が１．５Ｅ＋６〜１．０Ｅ＋１２となるように調整することを特徴とする１３〜１５のいずれかに記載の乳酸菌含有飲食品の食感改善方法。
The present invention relates to the following.
1. Component A and / or B and Component C and D are included, (A + B) / (A + B + C) is 0.001 or more, and (A + B) / D (pieces / g) is 1.5E + 6 to 1.0E + 12 A food and drink containing lactic acid bacteria with improved texture and whey float.
Ingredient A: Enterococcus faecalis (viable) (units / g)
Component B: Enterococcus faecalis (bactericidal) (pieces / g)
Component C: Lactic acid bacteria (live bacteria) other than Enterococcus faecalis (units / g)
Component D: Non-fat milk solid content (g / g)
2. The component C contains a lactic acid bacterium selected from lactic acid bacteria belonging to the genus Lactobacillus, Lactococcus, Pediococcus, Leuconostoc, Bifidobacterium, Streptococcus and Enterococcus 1 The food-drinks containing lactic acid bacteria described in 1.
3. The said component A and / or B are 1.0E + 6-1.0E + 11 (pieces / g), The lactic acid bacteria containing food-drinks of 1 or 2 characterized by the above-mentioned.
4). The component A, C, or D is included, A / (A + C) is 0.001 or more, and A / D is 1.5E + 6 to 1.0E + 12. Lactic acid bacteria-containing food and drink.
5. Component A and / or B and Component C and D are contained, and (A + B) / (A + B + C) is adjusted to a ratio of 0.001 or more, and (A + B) / D (pieces / g) is The manufacturing method of the food-drinks containing lactic acid bacteria with improved food texture and whey float, characterized by adjusting so that it may become 1.5E + 6-1.0E + 12.
Ingredient A: Enterococcus faecalis (viable) (units / g)
Component B: Enterococcus faecalis (bactericidal) (pieces / g)
Component C: Lactic acid bacteria (live bacteria) other than Enterococcus faecalis (units / g)
Component D: Non-fat milk solid content (g / g)
6). Component C contains a lactic acid bacterium selected from lactic acid bacteria belonging to the genus Lactobacillus, Lactococcus, Pediococcus, Leuconostoc, Bifidobacterium, Streptococcus and Enterococcus 5 The manufacturing method of lactic acid bacteria containing food-drinks of description.
7). The method for producing a food or drink containing lactic acid bacteria according to 5 or 6, wherein the component A and / or B is adjusted to 1.0E + 6 to 1.0E + 11 (pieces / g).
8). Components A, C and D are contained, A / (A + C) is adjusted to a ratio of 0.001 or more, and A / D (pieces / g) is 1.5E + 6 to 1.0E + 12. The method for producing a food or drink containing lactic acid bacteria according to any one of 5 to 7, wherein
9. Component A and / or B and Component C and D are contained, and (A + B) / (A + B + C) is adjusted to a ratio of 0.001 or more, and (A + B) / D (pieces / g) is The property stabilization method of the food-drinks containing lactic acid bacteria characterized by adjusting so that it may become 1.5E + 6-1.0E + 12.
Ingredient A: Enterococcus faecalis (viable) (units / g)
Component B: Enterococcus faecalis (bactericidal) (pieces / g)
Component C: Lactic acid bacteria (live bacteria) other than Enterococcus faecalis (units / g)
Component D: Non-fat milk solid content (g / g)
10. The component C contains a lactic acid bacterium selected from lactic acid bacteria belonging to the genus Lactobacillus, Lactococcus, Pediococcus, Leuconostoc, Bifidobacterium, Streptococcus and Enterococcus 9 The property stabilization method of the lactic acid bacteria containing food / beverage products as described in 1 ..
11. The method for stabilizing properties of a lactic acid bacteria-containing food or beverage according to 9 or 10, wherein the component A and / or B is adjusted to 1.0E + 6 to 1.0E + 11 (pieces / g).
12 Components A, C and D are contained, A / (A + C) is adjusted to a ratio of 0.001 or more, and A / D (pieces / g) is 1.5E + 6 to 1.0E + 12. The property stabilization method of the food-drinks containing lactic acid bacteria in any one of 9-11 characterized by adjusting to.
13. Component A and / or B and Component C and D are contained, and (A + B) / (A + B + C) is adjusted to a ratio of 0.001 or more, and (A + B) / D (pieces / g) is A method for improving the texture of a lactic acid bacteria-containing food or drink, characterized by adjusting to 1.5E + 6 to 1.0E + 12.
Ingredient A: Enterococcus faecalis (viable) (units / g)
Component B: Enterococcus faecalis (bactericidal) (pieces / g)
Component C: Lactic acid bacteria (live bacteria) other than Enterococcus faecalis (units / g)
Component D: Non-fat milk solid content (g / g)
14 Component C includes lactic acid bacteria selected from lactic acid bacteria belonging to the genus Lactobacillus, Lactococcus, Pediococcus, Leuconostoc, Bifidobacterium, Streptococcus and Enterococcus 13 The texture improvement method of the lactic acid bacteria containing food / beverage products as described in 1 ..
15. The method for improving the texture of lactic acid bacteria-containing food or beverage according to 13 or 14, wherein the component A and / or B is adjusted to 1.0E + 6 to 1.0E + 11 (pieces / g).
16. The components A and C are contained so that A / (A + C) is a ratio of 0.001 or more, and A / D (pieces / g) is adjusted to 1.5E + 6 to 1.0E + 12. The food texture improving method for lactic acid bacteria-containing food or drink according to any one of 13 to 15, wherein
本発明によれば、製造後、流通・販売時の経時において食感が良好であり、ホエー浮が抑制された乳酸菌含有飲食品を提供することができる。 According to the present invention, it is possible to provide a lactic acid bacterium-containing food or beverage that has a good texture over time after distribution and sales and has suppressed whey float.
以下、本発明の実施形態について説明する。
本発明は、エンテロコッカス・フェカリス菌を、全菌数に対して０．００１以上の比率で含有することを特徴とする乳酸菌含有飲食品及びその製造方法に関するものであり、性状安定化及び食感改善方法に関するものである。
Hereinafter, embodiments of the present invention will be described.
The present invention relates to a lactic acid bacteria-containing food and drink and a method for producing the same, characterized by containing Enterococcus faecalis bacteria in a ratio of 0.001 or more with respect to the total number of bacteria, and stabilizing properties and improving texture It is about the method.
（エンテロコッカス・フェカリス菌）
エンテロコッカス・フェカリス（Enterococcus faecalis）菌は、所謂腸球菌と称される乳酸菌であり、桿状の形を有する乳酸桿菌と比較すると、約１／５程度と非常に微細な粒形を成していることから、一度に多量の経口摂取が可能であるという特徴を有している。また、エンテロコッカス・フェカリス菌は、複数の株種を有しており、株種によって生理活性作用の強さ等も異なるが、殺菌された死滅菌であっても腸内細菌の餌となることによる整腸作用があり、近年においては免疫力向上、抗アレルギー作用等、身体に対して好ましい様々な機能を備えていることが報告されている。
(Enterococcus faecalis)
Enterococcus faecalis is a lactic acid bacterium called so-called enterococci and has a very fine particle shape of about 1/5 compared to a lactic acid bacterium having a rod-like shape. Therefore, a large amount of oral intake is possible at a time. In addition, Enterococcus faecalis has a plurality of strains, and the strength of the physiological activity varies depending on the strain, but even if it is killed by sterilization, it becomes a food for enteric bacteria. In recent years, it has been reported that it has various functions that are favorable to the body, such as improving immunity and antiallergic activity.
（エンテロコッカス・フェカリス菌の含有比率）
エンテロコッカス・フェカリス菌は、飲食品に含まれるエンテロコッカス・フェカリス菌（生菌）（個／ｇ）を成分Ａ、エンテロコッカス・フェカリス菌（殺菌体）（個／ｇ）を成分Ｂ、エンテロコッカス・フェカリス菌以外の乳酸菌（生菌）（個／ｇ）を成分Ｃとした場合に、飲食品中の乳酸菌数に対するフェカリス菌の比率［（Ａ＋Ｂ）／（Ａ＋Ｂ＋Ｃ）］が０．００１以上となるように添加されることを特徴とする。この範囲に調整することにより、他の菌による影響を大きく受けることなく食感を良好に保ち、ホエー浮を抑制する効果を生じさせることが可能となる。好ましくは０．００１〜０．９９、さらに好ましくは０．０１〜０．９８、最も好ましくは０．０１４〜０．９５の比率となるようにエンテロコッカス・フェカリス菌を含有させることにより、経時後であっても乳酸菌含有飲食品の食感を滑らかにし、ホエー浮を抑制することが可能となる。
これらの比率の調整は、乳又は乳製品を原料としてこれらを乳酸菌発酵させて得られる発酵乳や、乳酸菌乾燥粉末等を適宜配合することで、本発明の乳酸菌含有飲食品に所定の菌数の乳酸菌を含有させることができる。
(Content ratio of Enterococcus faecalis)
Enterococcus faecalis is Enterococcus faecalis (viable) (individuals / g) in component A, Enterococcus faecalis (disinfectant) (individuals / g) in component B, other than Enterococcus faecalis When lactic acid bacteria (live bacteria) (cells / g) of the above are used as component C, the ratio [(A + B) / (A + B + C)] of Fecalis to the number of lactic acid bacteria in food and drink is added to be 0.001 or more. It is characterized by that. By adjusting to this range, it is possible to maintain an excellent texture without being greatly affected by other bacteria and to produce an effect of suppressing whey float. By containing Enterococcus faecalis in a ratio of preferably 0.001 to 0.99, more preferably 0.01 to 0.98, most preferably 0.014 to 0.95, Even if it exists, the food texture of lactic acid bacteria containing food-drinks can be made smooth, and it becomes possible to suppress whey floating.
These ratios are adjusted by appropriately blending fermented milk obtained by fermenting these with lactic acid bacteria using milk or dairy products as a raw material, dry powder of lactic acid bacteria, etc. Lactic acid bacteria can be included.
（無脂乳固形分に対するエンテロコッカス・フェカリス菌の含有比率）
エンテロコッカス・フェカリス菌（個／ｇ）は、飲食品の無脂乳固形分量（ｇ／ｇ）を成分Ｄとした場合に、（Ａ＋Ｂ）／Ｄ（個／ｇ）が１．５Ｅ＋６〜１．０Ｅ＋１２（個／ｇ）の比率となるように添加する。この範囲に調整することにより、他の乳成分による影響を大きく受けることなく食感を良好に保ち、ホエー浮を抑制する効果を生じさせることが可能となる。好ましくは（Ａ＋Ｂ）／Ｄ（個／ｇ）が１．０Ｅ＋７〜２．０Ｅ＋１１（個／ｇ）、さらに好ましくは１．２Ｅ＋７〜１．８Ｅ＋１１（個／ｇ）の比率となるように添加することにより、経時後であっても乳酸菌含有飲食品の食感を滑らかにし、ホエー浮を抑制することが可能となる。
これらの比率の調整は、発酵乳や乳酸菌乾燥粉末等を適宜配合する他、無脂乳固形分を含有する乳若しくは乳製品等の乳原料等を適宜配合することにより行うことが可能である。
(Content ratio of Enterococcus faecalis to non-fat milk solids)
Enterococcus faecalis (pieces / g) has a non-fat milk solid content (g / g) of a food or drink as component D, and (A + B) / D (pieces / g) is 1.5E + 6 to 1.0E + 12 Add so that the ratio is (pieces / g). By adjusting to this range, it is possible to produce an effect of maintaining good texture and suppressing whey floating without being greatly affected by other milk components. Preferably, (A + B) / D (pieces / g) is added to a ratio of 1.0E + 7 to 2.0E + 11 (pieces / g), more preferably 1.2E + 7 to 1.8E + 11 (pieces / g). Thus, even after lapse of time, it is possible to smooth the texture of the lactic acid bacteria-containing food and drink and suppress whey float.
These ratios can be adjusted by appropriately blending fermented milk, dry powder of lactic acid bacteria, and the like, and appropriately blending milk raw materials such as milk or dairy products containing non-fat milk solids.
（エンテロコッカス・フェカリス菌数）
エンテロコッカス・フェカリス菌は、飲食品中に１．０Ｅ＋６〜１．０Ｅ＋１１（個／ｇ）となるように添加されることを特徴とする。この範囲に調整することにより、食感を良好に保ち、ホエー浮を抑制する効果を生じさせることが可能となる。好ましくは飲食品中に１．０Ｅ＋６〜２．０Ｅ＋１０（個／ｇ）、さらに好ましくは１．２Ｅ＋６〜１．６＋Ｅ１０（個／ｇ）の比率となるように添加することにより、経時後であっても乳酸菌含有飲食品の食感を滑らかにし、ホエー浮を抑制することが可能となる。
これらの比率の調整は、乳又は乳製品を原料としてこれらを乳酸菌発酵させて得られる発酵乳や、乳酸菌乾燥粉末等を適宜配合することで、本発明の乳酸菌含有飲食品に所定の菌数の乳酸菌を含有させることができる。
(Enterococcus faecalis count)
Enterococcus faecalis is characterized by being added so as to be 1.0E + 6 to 1.0E + 11 (pieces / g) in food and drink. By adjusting to this range, it is possible to produce an effect of maintaining a good texture and suppressing whey floating. Preferably, it is after the passage of time by adding to the food and drink so that the ratio is 1.0E + 6 to 2.0E + 10 (pieces / g), more preferably 1.2E + 6 to 1.6 + E10 (pieces / g). Can also smoothen the texture of lactic acid bacteria-containing foods and drinks and suppress whey float.
These ratios are adjusted by appropriately blending fermented milk obtained by fermenting these with lactic acid bacteria using milk or dairy products as a raw material, dry powder of lactic acid bacteria, etc. Lactic acid bacteria can be included.
（乳酸菌含有飲食品）
本発明の乳酸菌含有飲食品は、乳酸菌含有飲食品とは、無脂乳固形分が８％（０．０８ｇ／ｇ）以上の牛乳、羊乳を原料としたヨーグルト等の「はっ酵乳」、無脂乳固形分が３〜８％（０．０３〜０．０８ｇ／ｇ）のいわゆる「乳酸菌飲料」、無脂乳固形分が３％（０．０３ｇ／ｇ）未満の「乳製品乳酸菌飲料」、乳を含む、清涼飲料水等を広く包含するものである。本発明の乳酸菌含有飲食品において、乳酸菌は、エンテロコッカス・フェカリス菌を含めて１．０×１０５〜２．４×１０１２個／１００ｇ、好ましくは１．０×１０６〜１．０×１０１２個／１００ｇ、更に好ましくは１．０×１０７〜１．０×１０１１個／１００ｇ含有する。乳酸菌含有飲食品に含まれる乳酸菌は、生菌の状態でも殺菌体の状態でもよい。好ましくは生菌の状態で包含されていると、乳酸菌生菌由来の人体に有用な生理活性作用を享受することができる。従来、生菌を用いた場合には品質が劣化するなどの問題があったが、本発明は、特に生菌を用いた場合にもその効果が発揮される。
(Lactic acid bacteria-containing food and drink)
The lactic acid bacteria-containing food / beverage product of the present invention is different from the lactic acid bacteria-containing food / beverage products in terms of “fermented milk” such as yogurt made from milk or sheep milk having a non-fat milk solid content of 8% (0.08 g / g) or more. So-called “lactic acid bacteria beverage” having a solid content of fat milk of 3 to 8% (0.03 to 0.08 g / g), “dairy lactic acid bacteria beverage” having a solid content of nonfat milk of less than 3% (0.03 g / g) Widely includes soft drinks, including milk. In the food and drink containing lactic acid bacteria of the present invention, the lactic acid bacteria are 1.0 × 10 5 to 2.4 × 10 12 cells / 100 g including Enterococcus faecalis, preferably 1.0 × 10 6 to 1.0 × 10. 12 pieces / 100 g, more preferably 1.0 × 10 7 to 1.0 × 10 11 pieces / 100 g. The lactic acid bacterium contained in the lactic acid bacterium-containing food or drink may be in a live state or a sterilized state. When it is preferably included in the state of a living bacterium, a physiologically active action useful for a human body derived from a living lactic acid bacterium can be enjoyed. Conventionally, when live bacteria are used, there is a problem that the quality is deteriorated. However, the present invention is particularly effective when live bacteria are used.
（乳酸菌）
本実施形態において使用される乳酸菌は、一般的に食品に用いられるその他の菌種の使用ついて特に制限は無く、例えば、ラクトバチルス（Lactobacillus）属、ラクトコッカス（Lactococcus）属、ペディオコッカス（Pediococcus）属、ロイコノストック（Leuconostoc）属、ビフィドバクテリウム（Biffidobacterium）属、ストレプトコッカス（Streptococcus）属及びエンテロコッカス（Enterococcus）属に属する乳酸菌等を使用することができ、またこれらの乳酸菌を複数組み合わせて使用しても良い。本実施形態においては、乳又は乳製品を原料としてこれらを乳酸菌発酵させて得られる発酵乳や、乳酸菌乾燥粉末等を適宜配合することで、本発明の乳酸菌含有飲食品に所定の菌数の乳酸菌を含有させることができる。
(Lactic acid bacteria)
The lactic acid bacteria used in the present embodiment are not particularly limited with respect to the use of other bacterial species generally used in foods. For example, the genus Lactobacillus, Lactococcus, Pediococcus (Pediococcus) ) Genus, Leuconostoc genus, Biffidobacterium genus, Streptococcus genus and Enterococcus genus lactic acid bacteria can be used, and these lactic acid bacteria can be used in combination. May be used. In the present embodiment, lactic acid bacteria having a predetermined number of bacteria are added to the lactic acid bacteria-containing food and drink of the present invention by appropriately blending fermented milk obtained by fermenting these with lactic acid bacteria using milk or dairy products as raw materials, and lactic acid bacteria dry powder. Can be contained.
（菌体数）
なお、乳酸菌の菌体数は、エンテロコッカス・フェカリス菌の生菌及び死滅菌と、エンテロコッカス・フェカリス菌以外の乳酸菌（生菌）の菌体数の合計であり、常法に従って検鏡計数法、濁度法、コールカウンター法、酵素結合免疫吸着測定法（ＥＬＩＳＡ法やＳＹＢＲ Ｇｒｅｅｎ法）等により測定することが可能である。なお、本発明の乳酸菌含有飲食品における乳酸菌の菌体数は、上述の発酵乳や乳酸菌乾燥粉末等を適当量配合することで、所望の数値範囲に調整することができる。
(Number of cells)
The number of lactic acid bacteria is the sum of the number of living and dead sterilized Enterococcus faecalis bacteria and the number of lactic acid bacteria (viable bacteria) other than Enterococcus faecalis. It can be measured by a degree method, a call counter method, an enzyme-linked immunosorbent assay (ELISA method or SYBR Green method) and the like. In addition, the number of lactic acid bacteria in the lactic acid bacteria-containing food / beverage product of the present invention can be adjusted to a desired numerical range by blending appropriate amounts of the above-described fermented milk, lactic acid bacteria dry powder, and the like.
（無脂乳固形分）
また、無脂乳固形分は、乳又は乳製品等の乳原料から水分及び乳脂肪分を除いた固形分をいう。乳原料としては、ウシ、ウマ、ヤギ、ヒツジ等の哺乳動物の乳のほか、脱脂粉乳、全粉乳、バターミルク粉、無糖練乳、脱脂加糖練乳、全脂加糖練乳等の乳製品が挙げられ、これらを水に還元したものも含まれる。これらの乳若しくは乳製品等の乳原料、又は乳酸菌を含有させるために使用する発酵乳等及び／又は乳酸菌乾燥粉末等を適宜配合することで、本発明の乳酸菌含有飲食品に乳酸菌を含有させることができる。
(Non-fat milk solids)
The non-fat milk solid content refers to a solid content obtained by removing moisture and milk fat from milk raw materials such as milk or dairy products. Examples of dairy ingredients include milk from mammals such as cows, horses, goats, and sheep, as well as dairy products such as skim milk powder, whole milk powder, butter milk powder, sugar-free condensed milk, skim-free sweetened condensed milk, and whole-fat sweetened condensed milk. In addition, those obtained by reducing these to water are also included. The lactic acid bacteria-containing food / beverage product of the present invention may contain lactic acid bacteria by appropriately blending milk raw materials such as milk or dairy products, or fermented milk used to contain lactic acid bacteria and / or dry powder of lactic acid bacteria. Can do.
また、無脂乳固形分の含有量は、一般に「乳及び乳製品の成分規格等に関する省令」（昭和２６年１２月２７日厚生省令第５２号）に記載された方法により測定される。本発明の乳酸菌含有飲食品における無脂乳固形分の含有量は、上述した乳又は乳製品等の乳原料や発酵乳、若しくは脱脂粉乳等を適当量配合することで、所望の数値範囲に設定することができる。 The content of non-fat milk solids is generally measured by the method described in “Ministerial Ordinance on Component Standards of Milk and Dairy Products” (December 27, 1951, Ministry of Health and Welfare Ordinance No. 52). The content of non-fat milk solids in the lactic acid bacteria-containing food and drink of the present invention is set to a desired numerical range by blending appropriate amounts of the above-described milk raw materials such as milk or dairy products, fermented milk, or skim milk powder. can do.
また、本発明の乳酸菌含有飲食品においては、無脂乳固形分の含有量を０．０１〜０．２０ｇ／ｇ（１．０〜２０．０質量％）に調整する。０．０１〜０．１７ｇ／ｇ（１．０〜１７．０質量％）であることが好ましく、０．０５〜０．１５ｇ／ｇ（５．０〜１５．０質量％）であることがさらに好ましく、０．０８〜０．１３（８．０〜１３．０質量％）であることが最も好ましい。無脂乳固形分の含有量がこの範囲にあることにより、本発明の乳酸菌含有飲食品の滑らかなテクスチャーやコクを有するものとなる。 Moreover, in the lactic acid bacteria containing food / beverage products of this invention, content of non-fat milk solid content is adjusted to 0.01-0.20 g / g (1.0-20.0 mass%). It is preferably 0.01 to 0.17 g / g (1.0 to 17.0% by mass), and 0.05 to 0.15 g / g (5.0 to 15.0% by mass). More preferably, it is most preferably 0.08 to 0.13 (8.0 to 13.0 mass%). By having the non-fat milk solid content in this range, the lactic acid bacteria-containing food or drink of the present invention has a smooth texture and richness.
また、本発明の乳酸菌含有飲食品には、増粘剤として発酵セルロース、大豆多糖類の他、ペクチン、ガラクトマンナン類、ゼラチン、コラーゲン、寒天、アルギン酸ナトリウム、アラビアガム、タマリンドシードガム、カラギーナン、キサンタンガム、ジェランガム等が添加可能である。
また、その他、公知の飲料に含まれる材料（成分）、例えば、増粘剤、ビタミン類、甘味付与剤、酸味料、香料、ミネラル分、機能性成分等を、本実施形態による効果を損なわない範囲で配合してもよい。
The lactic acid bacterium-containing food and drink of the present invention include fermented cellulose and soybean polysaccharide as a thickener, pectin, galactomannans, gelatin, collagen, agar, sodium alginate, gum arabic, tamarind seed gum, carrageenan, and xanthan gum. Gellan gum and the like can be added.
In addition, other materials (components) contained in known beverages, such as thickeners, vitamins, sweeteners, acidulants, fragrances, minerals, functional ingredients, etc., do not impair the effects of this embodiment. You may mix | blend in the range.
また、本発明の乳酸菌含有飲食品に使用する水は、飲用に適した水であればよく、例えば、純水、硬水、軟水、イオン交換水等のほか、これらの水を脱気処理した脱気水等が挙げられる。 The water used in the lactic acid bacteria-containing food or drink of the present invention may be water that is suitable for drinking. For example, pure water, hard water, soft water, ion-exchanged water, etc. Examples include air and water.
この他、ビタミン類としては、例えば、ビタミンＣ、ビタミンＥ、ビタミンＤ、ビタミンＫ及びビタミンＢ群等が本実施形態による効果を損なわない範囲で添加可能である。 In addition, as vitamins, for example, vitamin C, vitamin E, vitamin D, vitamin K, vitamin B group, and the like can be added as long as the effects of the present embodiment are not impaired.
また、本発明の乳酸菌含有飲食品に添加する甘味付与剤としては、糖類又は甘味料を使用することができ、糖類としては、例えば、ショ糖、果糖、ブドウ糖、果糖ブドウ糖液糖、還元麦芽糖、乳糖等が挙げられる。甘味料としては、例えば、砂糖、グラニュー糖、異性化糖、キシリトール、パラチノース、エリスリトール等のほか、アスパルテーム、アセスルファムカリウム、ネオテーム、ステビア抽出物、サッカリン、スクラロース等の高甘味度甘味料が挙げられる。また、オリゴ糖などの糖質や、ソルビトール等の糖アルコールを含んでいてもよいし、シュガーレスバルク甘味料、バルク砂糖甘味料等を含んでいてもよい。 In addition, as a sweetening agent to be added to the lactic acid bacteria-containing food or drink of the present invention, saccharides or sweeteners can be used. Examples of the saccharide include sucrose, fructose, glucose, fructose glucose liquid sugar, reduced maltose, Examples include lactose. Examples of the sweetener include sugar, granulated sugar, isomerized sugar, xylitol, palatinose, erythritol and the like, as well as high-intensity sweeteners such as aspartame, acesulfame potassium, neotame, stevia extract, saccharin, and sucralose. In addition, sugars such as oligosaccharides and sugar alcohols such as sorbitol may be included, and sugarless bulk sweeteners, bulk sugar sweeteners, and the like may be included.
また、本発明の乳酸菌含有飲食品に添加する酸味料としては、例えば、クエン酸、クエン酸三ナトリウム、アジピン酸、グルコン酸、コハク酸、酒石酸、乳酸、フマル酸、リンゴ酸、又はそれらの塩類が挙げられ、中でも、クエン酸、クエン酸三ナトリウム、乳酸、リンゴ酸、酒石酸、アジピン酸等が好ましく、乳酸や、クエン酸が特に好ましい。 Examples of the acidulant added to the lactic acid bacteria-containing food and drink of the present invention include, for example, citric acid, trisodium citrate, adipic acid, gluconic acid, succinic acid, tartaric acid, lactic acid, fumaric acid, malic acid, or salts thereof. Among them, citric acid, trisodium citrate, lactic acid, malic acid, tartaric acid, adipic acid and the like are preferable, and lactic acid and citric acid are particularly preferable.
また、本発明の乳酸菌含有飲食品に添加する香料としては、例えば、乳又は乳製品から得られる香料、柑橘その他果実から抽出した香料、植物の種実、根茎、木皮、葉等又はこれらの抽出物、合成香料等が挙げられる。 In addition, as the fragrance added to the lactic acid bacteria-containing food and drink of the present invention, for example, fragrance obtained from milk or dairy products, fragrance extracted from citrus and other fruits, plant seeds, rhizomes, bark, leaves, etc., or extracts thereof And synthetic fragrances.
また、本発明の乳酸菌含有飲食品に添加するミネラル分としては、例えば、カルシウム、カリウム、クロム、銅、フッ素、ヨウ素、鉄、マグネシウム、マンガン、リン、セレン、ケイ素、モリブデン及び亜鉛等が挙げられる。 Examples of the mineral added to the lactic acid bacteria-containing food and drink of the present invention include calcium, potassium, chromium, copper, fluorine, iodine, iron, magnesium, manganese, phosphorus, selenium, silicon, molybdenum, and zinc. .
また、本発明の乳酸菌含有飲食品に添加する機能性成分としては、例えば、難消化性デキストリンや、イヌリンなどの食物繊維類、コラーゲン、コンドロイチン硫酸、グルコサミン、ヒアルロン酸、プラセンタ、牡蠣エキス、キトサン、プロポリス、ローヤルゼリー、トコフェロール、ポリフェノール、梅エキス、アロエ、霊芝、アガリクス等が挙げられる。 Examples of the functional ingredient added to the lactic acid bacteria-containing food or drink of the present invention include, for example, indigestible dextrin, dietary fibers such as inulin, collagen, chondroitin sulfate, glucosamine, hyaluronic acid, placenta, oyster extract, chitosan, Examples include propolis, royal jelly, tocopherol, polyphenol, plum extract, aloe, ganoderma, agaricus and the like.
また、本発明の乳酸菌含有飲食品には、その他、各種エステル類、乳化剤、保存料、調味料、着色料（色素）、油、ｐＨ調整剤、品質安定剤等を含有してもよい。 In addition, the lactic acid bacteria-containing food and drink of the present invention may contain various esters, emulsifiers, preservatives, seasonings, colorants (pigments), oils, pH adjusters, quality stabilizers, and the like.
本発明の乳酸菌含有飲食品は、ｐＨが３．０〜４．８であることが好ましく、３．５〜４．５であることがより好ましい。乳酸菌含有飲食品におけるｐＨがこの範囲にあることで、発酵乳に由来するヨーグルト様の爽やかな風味と、乳性飲料のボディ感との両立が可能となる。なお、乳酸菌含有飲食品のｐＨは、常法に従ってｐＨメーターにて測定することができる。また、本発明の乳酸菌含有飲食品のｐＨは、使用する発酵乳やｐＨ調整剤の配合量、また発酵乳の発酵度合い等を調整することにより、前記範囲に設定することが可能である。 The lactic acid bacteria-containing food / beverage product of the present invention preferably has a pH of 3.0 to 4.8, more preferably 3.5 to 4.5. When pH in lactic acid bacteria containing food / beverage products exists in this range, coexistence with the refreshing flavor like yogurt derived from fermented milk and the body feeling of a dairy drink is attained. In addition, pH of lactic acid bacteria containing food-drinks can be measured with a pH meter according to a conventional method. Moreover, pH of the lactic acid bacteria containing food / beverage products of this invention can be set to the said range by adjusting the compounding quantity of fermented milk and pH adjuster to be used, the fermentation degree of fermented milk, etc.
本発明の乳酸菌含有飲食品は、適度な酸味を付与する観点から、生産直後〜１０℃で２４時間保管時の酸度が０．７０〜１．２０％であることが好ましく、０．７１〜１．１０％であることがより好ましい。本発明において酸度とは、乳酸の質量に換算した酸の濃度（質量％）を意味するものである。また、２週間経過後の時点における発酵乳の酸度は、酸味が強すぎることによる発酵乳の風味の劣化を防止する観点から、１０℃の温度下で、好ましくは０．７０〜１．４％、さらに好ましくは０．８０〜１．３２％である。
なお、飲料の場合は、クエン酸酸度換算の酸度（クエン酸酸度）において、０．１〜０．５％とすることがこのましい。クエン酸酸度と乳酸酸度の関係は、クエン酸酸度１に対して、乳酸酸度０．７１１に相当する。
From the viewpoint of imparting an appropriate acidity, the lactic acid bacteria-containing food or drink of the present invention preferably has an acidity of 0.70 to 1.20% immediately after production at 10 ° C. for 24 hours, and 0.71 to 1%. More preferably, it is 10%. In the present invention, the acidity means the acid concentration (% by mass) converted to the mass of lactic acid. In addition, the acidity of the fermented milk at the time point after two weeks has passed, preferably at a temperature of 10 ° C., preferably 0.70 to 1.4% from the viewpoint of preventing the deterioration of the flavor of the fermented milk due to the too strong acidity. More preferably, it is 0.80 to 1.32%.
In the case of beverages, it is preferable to set the acidity in terms of citric acidity (citric acidity) to 0.1 to 0.5%. The relationship between the citric acidity and the lactic acidity corresponds to a lactic acidity of 0.711 with respect to a citric acidity of 1.
また、本発明の乳酸菌含有飲食品は、Ｂｒｉｘ（Ｂｘ）が２．０〜２０であることが好ましく、４〜１７であることがより好ましい。ここで、Ｂｘは一般に糖度を表す指標であるが、本実施形態においては、乳酸菌含有飲食品における溶存固形分濃度の指標としてＢｘを用いることができる。本発明の乳酸菌含有飲食品は、Ｂｘが前記範囲にあることで、十分な発酵乳の風味を活かした乳酸菌含有飲食品を製造することができ、また甘味調整においても飲用に適した甘味が付与されたものとなる。なお、乳酸菌含有飲食品のＢｘは、常法に従って屈折計にて測定することができる。また、本発明の乳酸菌含有飲食品のＢｘは、使用する発酵乳、糖類、脱脂粉乳等の配合量を調整することにより、前記範囲に設定することが可能である。 Moreover, it is preferable that Brix (Bx) is 2.0-20, and, as for the lactic acid bacteria containing food-drinks of this invention, it is more preferable that it is 4-17. Here, Bx is generally an index representing sugar content, but in the present embodiment, Bx can be used as an index of dissolved solid content concentration in lactic acid bacteria-containing foods and drinks. The lactic acid bacterium-containing food / beverage product of the present invention can produce a lactic acid bacterium-containing food / beverage product that makes full use of the flavor of fermented milk, and imparts a sweetness suitable for drinking even when adjusting the sweetness. Will be. In addition, Bx of lactic acid bacteria containing food-drinks can be measured with a refractometer according to a conventional method. Moreover, Bx of the lactic acid bacteria containing food-drinks of this invention can be set to the said range by adjusting compounding quantities, such as fermented milk, saccharides, and skim milk powder to be used.
本発明の乳酸菌含有飲食品は、容器に充填された形で提供されることが好ましい。この場合において、使用される容器は、流通や小売りに一般的に用いられているものであれば特に制限はなく、例えば、プラスチック製、紙製、ガラス製、金属製、陶器製或いはその複合材料からなる容器を用いることができる。また、容器は、通常の手段により密封包装して流通等行うことが好ましい。飲料の場合は、金属缶、ＰＥＴボトル、金属箔やプラスチックフィルムと複合された紙容器、瓶等の通常用いられる飲料用容器であればよい。また、本発明の乳酸菌含有飲食品は、常温においても性状が安定し分離しにくいため、透明の飲料用容器（例えば、ＰＥＴボトル等）を用いても良い。なお、本発明の乳酸菌含有飲食品が容器に充填された容器詰飲料として提供される場合、通常は希釈せずにそのまま飲用できるものであるが、これに限定されるものではない。 The lactic acid bacteria-containing food or drink of the present invention is preferably provided in a form filled in a container. In this case, the container to be used is not particularly limited as long as it is generally used for distribution and retail. For example, plastic, paper, glass, metal, ceramic, or a composite material thereof A container made of can be used. Moreover, it is preferable that the container is sealed and packaged by a normal means for distribution or the like. In the case of a beverage, it may be a commonly used beverage container such as a metal can, a PET bottle, a paper container combined with a metal foil or a plastic film, or a bottle. In addition, since the lactic acid bacteria-containing food and drink of the present invention have stable properties and are difficult to separate even at room temperature, a transparent beverage container (for example, a PET bottle or the like) may be used. In addition, when the lactic acid bacteria containing food / beverage products of this invention are provided as a container-packed drink with which the container was filled, although it can normally be drunk without dilution, it is not limited to this.
本発明の乳酸菌含有飲食品は、前記所定の範囲内となるようにエンテロコッカス・フェカリス菌を含有する以外、従来公知の方法により製造することができる。例えば、水に、乳酸菌及び無脂乳固形分を含有する組成物並びにエンテロコッカス・フェカリス菌等を添加し、さらに所望により上述した他の成分を添加して攪拌し、必要に応じてｐＨの調整を行い、調合物を調製する。そして、その後均質化して殺菌処理を行い、容器に充填する工程により製造することができる。 The lactic acid bacteria-containing food / beverage product of the present invention can be produced by a conventionally known method except that it contains Enterococcus faecalis so as to be within the predetermined range. For example, a composition containing lactic acid bacteria and non-fat milk solids and Enterococcus faecalis is added to water, and the other ingredients described above are added and stirred as desired, and the pH is adjusted as necessary. To prepare the formulation. And it can manufacture by the process of homogenizing after that, performing a sterilization process, and filling a container.
（乳酸菌含有食品の製造方法）
本発明の乳酸菌含有食品は、一般的な乳酸菌含有飲食品の製造工程において前述の成分調整を行うことにより製造することができる。以下に一般的な乳酸菌含有飲食品の製造工程を記述するが、以下の工程に制限されるものではない。
（１）原料乳の調製
無脂乳固形分量を特定の範囲に調整するなど、生乳の成分を調整して乳酸菌含有食品に合う成分値及び性状に調製し、調乳とする。調製方法は従来から一般的に用いられる方法により行う。市販の加工用調乳を使用してもよい。
（２）調合
砂糖などの糖分、甘味料、寒天などの増粘剤、エンテロコッカス・フェカリス菌製剤（殺菌体）などを調乳に添加し、均一になるまで混合する。
（３）加温・殺菌
調合後の調乳を８０℃程度まで加温させ、８０〜８４℃で２０〜３０分殺菌処理を行う。最も好ましくは８０℃で２０分間である。
（４）冷却
殺菌後、植菌可能な温度である４０℃付近、好ましくは４０±２℃まで冷却する。
（５）植菌
事前にスターターとして培養した乳酸菌を、４０℃付近まで冷却された調乳に添加する。乳酸菌はエンテロコッカス・フェカリス菌（生菌）の他、サーモフィルス菌及びブルガリカス菌などの前述の乳酸菌が適宜選択され、それぞれの添加菌数も適宜調整される。本工程は殺菌終了後の添加になる為、スターターを計りとる際はコンタミネーションが無いよう十分注意する。培養中に製品を振動させると組織が壊れやすくなる為、静置培養を心掛ける。
（６）充填
プラスチック製カップなどの適当な容器に充填し、密封する。この充填工程は、発酵及び冷却後に行ってもよい。
（７）発酵
好ましくは４０±２℃の条件下で約４〜６時間維持し、酸度が０．５〜１．５、好ましくは０．６〜０．８程度になるまで発酵させる。
（８）冷却
冷水を掛けるなどの手段により、発酵が停止する温度、１０℃程度まで冷却させる。
飲料の場合はこれらの工程に加え、更に希釈工程、安定剤を添加する工程、殺菌工程などが適宜追加される。
(Method for producing lactic acid bacteria-containing food)
The lactic acid bacteria-containing food of the present invention can be produced by performing the above-described component adjustment in the production process of a general lactic acid bacteria-containing food or drink. Although the manufacturing process of the general lactic acid bacteria containing food / beverage products is described below, it is not restrict | limited to the following processes.
(1) Preparation of raw milk The raw milk components are adjusted to a specific range such as adjusting the solid content of non-fat milk to a specific range, and adjusted to component values and properties suitable for lactic acid bacteria-containing foods to prepare milk. The preparation method is performed by a conventionally used method. Commercially available milk for processing may be used.
(2) Add sugar such as formula sugar, sweeteners, thickeners such as agar, enterococcus faecalis preparation (bactericidal), etc. to the formula and mix until uniform.
(3) Heating and sterilizing the prepared milk to about 80 ° C., and sterilizing at 80 to 84 ° C. for 20 to 30 minutes. Most preferably, it is 20 minutes at 80 ° C.
(4) After cooling and sterilization, it is cooled to around 40 ° C., preferably 40 ± 2 ° C., which is the inoculatable temperature.
(5) Inoculation The lactic acid bacteria cultured as a starter in advance are added to the milk cooled to around 40 ° C. As the lactic acid bacteria, the aforementioned lactic acid bacteria such as Thermophilus bacteria and Bulgaricus bacteria are appropriately selected in addition to Enterococcus faecalis bacteria (viable bacteria), and the number of each added bacteria is appropriately adjusted. Since this process is added after the sterilization is completed, be careful not to contaminate the starter. Vibrating the product during culturing makes the tissue more fragile.
(6) Fill into a suitable container such as a filled plastic cup and seal. This filling step may be performed after fermentation and cooling.
(7) Fermentation Preferably it is maintained for about 4 to 6 hours under the condition of 40 ± 2 ° C. and fermented until the acidity is about 0.5 to 1.5, preferably about 0.6 to 0.8.
(8) Cool down to a temperature at which fermentation stops, about 10 ° C. by means such as applying cooling cold water.
In the case of beverages, in addition to these steps, a dilution step, a step of adding a stabilizer, a sterilization step, and the like are appropriately added.
以上説明した本発明の乳酸菌含有飲食品は、経時後であっても食感が良好であり、ホエー浮が抑制された乳酸菌含有飲食品を提供することが可能となる。 The lactic acid bacterium-containing food / beverage products of the present invention described above can provide a lactic acid bacterium-containing food / beverage product that has a good texture even after lapse of time and has suppressed whey float.
以上説明した実施形態は、本発明の理解を容易にするために記載されたものであって、本発明を限定するために記載されたものではない。したがって、前記実施形態に開示された各要素は、本発明の技術的範囲に属する全ての設計変更や均等物をも含む趣旨である。 The embodiment described above is described for facilitating understanding of the present invention, and is not described for limiting the present invention. Therefore, each element disclosed in the embodiment is intended to include all design changes and equivalents belonging to the technical scope of the present invention.
以下、実施例等により本発明をさらに具体的に説明するが、本発明の範囲はこれらの実施例等に限定されるものではない。 EXAMPLES Hereinafter, although an Example etc. demonstrate this invention further more concretely, the scope of the present invention is not limited to these Examples etc.
（実施例１）
無脂乳固形分量が９．７％となるように脱脂粉乳をイオン交換水に溶解させ、７０℃以上に加熱後、ホモジナイザー（１５０Ｋｇ／ｃｍ２）処理を行い、９０℃で５分間加熱殺菌後、冷却して調整乳とした。
調整乳に殺菌体のエンテロコッカス・フェカリス菌０．０４％（１．６＋９Ｅ個／g）（殺菌体）を添加し、８０℃で３０分殺菌後、４５℃まで冷却した。その後スターターとして、サーモフィルス菌液０．０２％（乳酸菌数１．０＋１０Ｅ個／ｇ）（生菌）及びブルガリカス菌液１．２５％（乳酸菌数１．０＋９Ｅ個／ｇ）（生菌）を添加し、１０分間攪拌した。攪拌した後、４０℃で３０分保管した。これをポリプロピレン製カップに約７０ｇずつ充填、シール後、発酵室にて４０℃で酸度が０．７０２になるまで維持した。その後、１０℃で２４時間維持したものを「スタート時」、スタート時から２週間経過したものを「２週間」として外観・酸味及び食感の評価を実施した。
(Example 1)
Skim milk powder is dissolved in ion-exchanged water so that the solid content of non-fat milk is 9.7%, heated to 70 ° C. or higher, homogenizer (150 Kg / cm 2) treatment is performed, and after heat sterilization at 90 ° C. for 5 minutes, Cooled to make adjusted milk.
Bactericidal Enterococcus faecalis 0.04% (1.6 + 9E / g) (sterilized body) (sterilized body) was added to the prepared milk, sterilized at 80 ° C. for 30 minutes, and then cooled to 45 ° C. Then, as a starter, 0.02% of thermophilus bacterial solution (1.0 + 10E lactic acid bacteria / g) (viable) and 1.25% bulgaricus bacterial solution (1.0 + 9E lactic acid bacteria / g) (viable) Added and stirred for 10 minutes. After stirring, it was stored at 40 ° C. for 30 minutes. About 70 g of this was filled in a polypropylene cup, sealed, and maintained in a fermentation chamber at 40 ° C. until the acidity reached 0.702. Thereafter, the appearance, sourness and texture were evaluated as “starting” when maintained at 10 ° C. for 24 hours and “2 weeks” after 2 weeks from the starting time.
（実施例２〜１３及び比較例１〜８）
実施例１のサンプル作成方法を基準とし、表１に示すように、それぞれの添加量を変更して、乳酸菌含有飲食品（サンプル）を作製した。
生菌のフェカリス菌を使用した実施例１１は、フェカリス菌（殺菌体）を添加しない代わりに、ブルガリカス菌とサーモフィラス菌の生菌に加え、フェカリス菌（生菌）を添加した以外は実施例１と同様であった。
比較例５は、フェカリス菌（殺菌体）の代わりにビフィズス菌（殺菌体）を添加した以外は実施例１と同様であった。
(Examples 2 to 13 and Comparative Examples 1 to 8)
Based on the sample preparation method of Example 1, as shown in Table 1, each addition amount was changed and the food / beverage products (sample) containing lactic acid bacteria were produced.
Example 11 using viable faecalis bacteria was carried out in the same manner as in Example 11 except that fecalis bacteria (live bacteria) were added in addition to viable bacteria of Bulgaricus and thermophilus bacteria instead of adding fecalis bacteria (bacteria). It was the same as 1.
Comparative Example 5 was the same as Example 1 except that Bifidobacteria (bactericidal) was added instead of Fecalis (bactericidal).
表１の配合に基づいて作製された実施例及び比較例サンプルの成分を、下記方法に基づいて分析・測定した。結果を表１に示す。
＜成分の測定方法＞
（酸度の測定方法）
０．２Ｎ水酸化ナトリウムによる中和滴定法にて測定した。
（菌数の測定方法）
サンプルを、コロニー数が３０から３００になるよう生理食塩水で稀釈し、希釈液１ｍＬをシャーレに入れ、その上にＢＣＰ加プレート寒天培地を流し込み、攪拌した。これを３７℃で７２±３時間培養し、黄変コロニーをカウントすることにより測定した。
フェカリス菌生菌の場合は、ＢＣＰ加プレート寒天培地に３％のＮaＯＨを添加した以外は同様の方法により測定した。
The components of Examples and Comparative Samples prepared based on the formulations shown in Table 1 were analyzed and measured based on the following methods. The results are shown in Table 1.
<Measurement method of component>
(Measurement method of acidity)
It measured by the neutralization titration method by 0.2N sodium hydroxide.
(Method for measuring the number of bacteria)
The sample was diluted with physiological saline so that the number of colonies was 30 to 300, and 1 mL of the diluted solution was put into a petri dish, and a BCP-added plate agar medium was poured onto it and stirred. This was cultured at 37 ° C. for 72 ± 3 hours and measured by counting yellowing colonies.
In the case of viable fecalis, the measurement was carried out in the same manner except that 3% NaOH was added to the BCP-added plate agar medium.
＜評価＞
実施例１〜１３及び比較例１〜８の各乳酸菌含有飲食品を用いて、品温１０℃における製造翌日の外観観察及び官能評価（スタート時）と、製造後２週間後の外観観察及び官能評価（２Ｗ）を実施し、風味の変化を評価した。結果を表１に示す。
<Evaluation>
Using the lactic acid bacteria-containing foods and drinks of Examples 1 to 13 and Comparative Examples 1 to 8, appearance observation and sensory evaluation (at the start) on the next day of manufacture at a product temperature of 10 ° C., appearance observation and sensory evaluation two weeks after manufacture Evaluation (2W) was carried out to evaluate the change in flavor. The results are shown in Table 1.
＜スタート時評価＞
冷蔵庫に保管して品温を１０℃にした各乳酸菌含有飲食品を、熟練した評価者５名に少量ずつ試食してもらい、外観及び食感について官能評価をした。各項目について、以下の評価基準に従い、５名の合議で◎、○、△及び×の４段階で評価した。
＜２週間経過時評価＞
冷蔵庫に２週間保管して品温を１０℃にした各乳酸菌含有飲食品を、熟練した審査官５名に外観の性状について評価してもらい、かつ少量ずつ試食してもらい、食感について官能評価をした。各項目について、以下の評価基準に従い、５名の合議で◎、○、△及び×の４段階で評価した。
<Evaluation at the start>
Each of the lactic acid bacteria-containing foods and drinks stored in a refrigerator and brought to a temperature of 10 ° C. was tasted by five skilled evaluators in small quantities, and subjected to sensory evaluation on appearance and texture. Each item was evaluated in four stages of ◎, ○, Δ, and × by a meeting of five persons according to the following evaluation criteria.
<Evaluation at the end of 2 weeks>
Each lactic acid bacteria-containing food and drink that was stored in a refrigerator for 2 weeks and kept at a temperature of 10 ° C. was evaluated by the 5 expert examiners for the appearance and tasted in small quantities, and the sensory evaluation of the texture Did. Each item was evaluated in four stages of ◎, ○, Δ, and × by a meeting of five persons according to the following evaluation criteria.
＜評価基準＞
＜外観＞
◎：ホエー浮がない。
○：ホエー浮がほとんどない。
△：ホエー浮が少し見られる。
×：ホエー浮がかなり見られる。
＜食感＞
◎：とても滑らかで良好な食感である。
○：やや滑らかで良好な食感である。
△：ややぼそぼそ感がある。
×：かなりぼそぼそ感があり、不適である。
<Evaluation criteria>
<Appearance>
A: There is no whey float.
○: Almost no whey float.
Δ: A little whey floating is seen.
X: Whey float is considerably seen.
<Food texture>
A: Very smooth and good texture.
○: Slightly smooth and good texture.
Δ: Slightly insensitive
X: Unsatisfactory because there is a feeling of looseness.
以上に示すとおり、エンテロコッカス・フェカリス菌を添加した実施例１〜１３は、エンテロコッカス・フェカリス菌を添加しなかった比較例と比較すると、食感が良好であり、ホエー浮が抑制されていた。また、実施例１〜１３は、２週間経過後も滑らかな食感を有し、ホエー浮がないか、あるいはほとんどない状態を維持していた。
この効果は、エンテロコッカス・フェカリス菌（個／ｇ）を、飲食品に含まれる全菌数に対して０．００１以上の比率となるように添加し、かつ飲食品の無脂乳固形分量（ｇ／ｇ）に対して１．５Ｅ＋６〜１．０Ｅ＋１２（個／ｇ）の比率となるように添加した飲食品において見られた。さらにエンテロコッカス・フェカリス菌を、１．０Ｅ＋６〜２．０Ｅ＋１１（個／ｇ）となるように添加した場合でより顕著であった。
以上より、本発明は食感が良好であり、ホエー浮が抑制された乳酸菌含有飲食品を提供することが可能となる。
As shown above, Examples 1 to 13 to which Enterococcus faecalis were added had a good texture and whey float was suppressed as compared with the comparative examples to which Enterococcus faecalis was not added. In addition, Examples 1 to 13 had a smooth texture even after two weeks had passed, and maintained a state where there was no whey float or almost none.
This effect is obtained by adding Enterococcus faecalis bacteria (cells / g) so that the ratio is 0.001 or more with respect to the total number of bacteria contained in the food and drink, and the amount of non-fat milk solids (g / G) was found in foods and drinks added to a ratio of 1.5E + 6 to 1.0E + 12 (pieces / g). Furthermore, it was more remarkable when Enterococcus faecalis was added so that it might become 1.0E + 6-2.0E + 11 (piece / g).
From the above, the present invention can provide a lactic acid bacterium-containing food or drink with a good texture and suppressed whey float.
本発明は、経時後であっても乳酸菌含有飲食品食感が良好であり、ホエー浮を抑制するため、特に乳酸菌の含有量が従来の乳酸菌飲料と比較して高含有である乳酸菌含有飲食品に対して特に好適である。 The present invention has a lactic acid bacteria-containing food and drink texture that is good even after lapse of time and suppresses whey float, so that the content of lactic acid bacteria is particularly high compared to conventional lactic acid bacteria drinks. Is particularly suitable.
Claims (12)
成分Ａ：エンテロコッカス・フェカリス菌（生菌）（個／ｇ）
成分Ｂ：エンテロコッカス・フェカリス菌（殺菌体）（個／ｇ）
成分Ｃ：エンテロコッカス・フェカリス菌以外の乳酸菌（生菌）（個／ｇ）
成分Ｄ：無脂乳固形分（ｇ／ｇ） Component A and / or B and Component C and D are included, (A + B) / (A + B + C) is 0.001 or more, and (A + B) / D (pieces / g) is 1.5E + 6 to 1.0E + 12 And a food / beverage product containing lactic acid bacteria with improved texture and whey float , wherein A / D (pieces / g) is 1.5E + 6 to 1.0E + 12 .
Ingredient A: Enterococcus faecalis (viable) (units / g)
Component B: Enterococcus faecalis (bactericidal) (pieces / g)
Component C: Lactic acid bacteria (live bacteria) other than Enterococcus faecalis (units / g)
Component D: Non-fat milk solid content (g / g)
成分Ａ：エンテロコッカス・フェカリス菌（生菌）（個／ｇ）
成分Ｂ：エンテロコッカス・フェカリス菌（殺菌体）（個／ｇ）
成分Ｃ：エンテロコッカス・フェカリス菌以外の乳酸菌（生菌）（個／ｇ）
成分Ｄ：無脂乳固形分（ｇ／ｇ） Component A and / or B and Component C and D are contained, and (A + B) / (A + B + C) is adjusted to a ratio of 0.001 or more, and (A + B) / D (pieces / g) is Lactic acid-containing food and drink with improved texture and whey float, characterized by being adjusted to 1.5E + 6 to 1.0E + 12 and A / D (pieces / g) to be 1.5E + 6 to 1.0E + 12 Product manufacturing method.
Ingredient A: Enterococcus faecalis (viable) (units / g)
Component B: Enterococcus faecalis (bactericidal) (pieces / g)
Component C: Lactic acid bacteria (live bacteria) other than Enterococcus faecalis (units / g)
Component D: Non-fat milk solid content (g / g)
成分Ａ：エンテロコッカス・フェカリス菌（生菌）（個／ｇ）
成分Ｂ：エンテロコッカス・フェカリス菌（殺菌体）（個／ｇ）
成分Ｃ：エンテロコッカス・フェカリス菌以外の乳酸菌（生菌）（個／ｇ）
成分Ｄ：無脂乳固形分（ｇ／ｇ） Component A and / or B and Component C and D are contained, and (A + B) / (A + B + C) is adjusted to a ratio of 0.001 or more, and (A + B) / D (pieces / g) is It is 1.5E + 6-1.0E + 12 , and it adjusts so that A / D (piece / g) may be 1.5E + 6-1.0E + 12 , The property stabilization method of the lactic acid bacteria containing food-drinks characterized by the above-mentioned.
Ingredient A: Enterococcus faecalis (viable) (units / g)
Component B: Enterococcus faecalis (bactericidal) (pieces / g)
Component C: Lactic acid bacteria (live bacteria) other than Enterococcus faecalis (units / g)
Component D: Non-fat milk solid content (g / g)
成分Ａ：エンテロコッカス・フェカリス菌（生菌）（個／ｇ）
成分Ｂ：エンテロコッカス・フェカリス菌（殺菌体）（個／ｇ）
成分Ｃ：エンテロコッカス・フェカリス菌以外の乳酸菌（生菌）（個／ｇ）
成分Ｄ：無脂乳固形分（ｇ／ｇ） Component A and / or B and Component C and D are contained, and (A + B) / (A + B + C) is adjusted to a ratio of 0.001 or more, and (A + B) / D (pieces / g) is It is 1.5E + 6-1.0E + 12 , and it adjusts so that A / D (piece / g) may be 1.5E + 6-1.0E + 12 , The food texture improvement method of lactic acid bacteria containing food-drinks characterized by the above-mentioned.
Ingredient A: Enterococcus faecalis (viable) (units / g)
Component B: Enterococcus faecalis (bactericidal) (pieces / g)
Component C: Lactic acid bacteria (live bacteria) other than Enterococcus faecalis (units / g)
Component D: Non-fat milk solid content (g / g)
The method for improving the texture of lactic acid bacteria-containing food or beverage according to claim 10 or 11 , wherein the component A and / or B is adjusted to 1.0E + 6 to 1.0E + 11 (pieces / g).
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