JP5975772B2 - Automatic analyzer - Google Patents

Description

  The present invention relates to an automatic analyzer that performs qualitative and quantitative analysis of biological samples such as blood and urine.

  A clinical test for analyzing a component contained in a biological sample such as blood or urine of a patient performs a qualitative / quantitative analysis of a target component in the sample by reacting the sample with a reagent. In this case, a liquid supply mechanism called a dispensing mechanism is used in order to supply a predetermined amount of the sample and reagent to the reaction container for reacting the sample and the reagent. As the liquid supply mechanism, the pressure in the nozzle that sucks the liquid using a syringe as a pressure changing means is set to a negative pressure, and after sucking the sucked liquid into the nozzle, the pressure in the nozzle is corrected using the pressure changing means. By setting the pressure, a predetermined amount of liquid is discharged into the reaction vessel.

  Since the nozzle dispenses different samples and different reagents, the nozzle is washed after aspirating and discharging one sample and reagent. Nozzle cleaning includes external cleaning in which a cleaning liquid is applied to the outside of the nozzle and cleaning, and internal cleaning in which the cleaning liquid is discharged from the nozzle to clean the inside of the nozzle. If sufficient cleaning is not performed in the nozzle cleaning process, the analysis result is affected. For this reason, Patent Document 1 describes a system that monitors the pressure and timing of cleaning water and issues a warning when it is not normal.

WO2010 / 038546

  The water for outer washing and inner washing is drawn into the automatic analyzer by a water supply pump via a water supply tank. Thereafter, the internal wash water becomes a high pressure of about 250 kPa in a pipe near the nozzle outlet, for example, through a gear pump. In order to sufficiently clean the inside of the nozzle, it is necessary to apply a pressure above a certain level. However, this pressure may decrease due to deterioration or failure of the flow path components.

  In addition to the causes of deterioration and failure of the flow channel components, a pressure drop in the flow channel can also occur due to user maintenance errors. Since the water tank is regularly cleaned, it has a removable structure. When removing it, the cock between the automatic analyzer and the water tank is closed. If the cock is not opened by mistake after cleaning, water is not supplied into the apparatus, and as a result, the pressure required for internal washing is not applied.

  An object of the present invention is to detect an abnormality of any one of a solenoid valve, a gear pump, and a water supply tank provided in a flow path of a dispensing nozzle by measuring an output of a pressure sensor in the dispensing nozzle. Also, the abnormality factor detected from the output value is determined.

  A typical example of the present invention is a dispensing nozzle for sucking and discharging a predetermined amount of liquid, a dispensing syringe for changing the pressure in the dispensing nozzle, the dispensing nozzle and the dispensing. A pressure sensor provided in a pipe connecting the syringe, a solenoid valve for separating a liquid flow path in the pipe, a water supply pump for supplying water to the dispensing nozzle, and a water supply for supplying water to the water supply pump In an automatic analyzer comprising a water supply tank and a gear pump for applying water pressure to the liquid in the dispensing nozzle, the output of the pressure sensor is measured, and based on the output, the solenoid valve, the gear pump, It is an automatic analyzer provided with a mechanism for detecting any abnormality of the water supply tank.

  Normalization can be performed by detecting any abnormality of the solenoid valve, the gear pump, or the water supply tank, and it is possible to prevent a defective cleaning of the dispensing probe due to these abnormalities. Therefore, the analysis accuracy of the automatic analyzer can be maintained. Further, by determining the cause of the abnormality, it is possible to properly normalize the abnormal part and shorten the apparatus stop time.

It is a figure explaining the flow path of the dispensing probe of the automatic analyzer which concerns on this invention. It is a figure explaining the pressure behavior of the pressure sensor in the dispensing probe of the automatic analyzer which concerns on this invention.

  FIG. 1 is an example of a configuration diagram of an automatic analyzer to which the present invention is applied. FIG. 1 is a diagram illustrating a flow path from a water supply tank 12 to a dispensing probe 1 of the automatic analyzer.

  A sample probe (dispensing nozzle) 1 is connected to a dispensing syringe 3 via a tube 2, and the inside thereof is filled with a liquid. The dispensing syringe 3 includes a cylinder 3a and a plunger 3b, and a dispensing syringe drive mechanism 4 is connected to the plunger 3b. The cylinder 3a is fixed, and the plunger 3b is driven up and down by the dispensing syringe drive mechanism 4, thereby performing the sample dispensing operation. Usually, since there are a plurality of types of sample probes, the electromagnetic valve 14 is connected to the gear pump in order to separate the flow path. A sample probe driving mechanism 5 is connected to the sample probe 1 so that the sample probe 1 can be moved to a predetermined position. The dispensing syringe drive mechanism 4 and the sample probe drive mechanism 5 are controlled by the control unit 6.

  The sample probe 1 is a probe for sucking and discharging a predetermined amount of liquid, and mainly sucks and discharges specimens such as blood and urine. The dispensing syringe 3 can change the pressure in the sample probe 1. The electromagnetic valve 14 serves to separate the liquid flow path in the pipe connecting the sample probe 1 and the dispensing syringe.

  Now, when the sample probe 1 is lowered by the sample probe driving mechanism 5 and reaches the liquid of the sample 8 in the sample container 7, the dispensing syringe 3 performs a suction operation by the dispensing syringe driving mechanism 4. When the sample suction operation is completed, the sample probe 1 moves to the sample discharge position 9, and the dispensing syringe 3 performs the discharge operation. After dispensing, the sample probe 1 moves to the washing position 10, the washing water 13 in the water supply tank 12 is drawn by the water supply pump 11, the pressure is made high by the gear pump 13, and the sample probe 1 can be washed. It is. The switching is performed by the electromagnetic valve 14, which is controlled by the control unit 6.

  The water supply pump 11 is a pump for supplying water to the dispensing nozzle, and the water supply tank 12 is a tank for supplying water to the water supply pump 11. The gear pump 13 is a pump for applying water pressure to the liquid in the dispensing nozzle.

  The pressure sensor 15 is connected to a dispensing channel system including the sample probe 1, the tube 2, and the dispensing syringe 3 through the branch block 16. The pressure sensor 15 can detect the pressure of the liquid in the dispensing flow path system. The pressure signal of the pressure sensor 15 is processed by the circuit 17.

FIG. 2 is a diagram for explaining the behavior of the pressure sensor targeted by the present invention. The lower figure shows the on / off state of the gear pump and the electromagnetic valve, the state of the apparatus, and the monitoring items, and the upper figure shows the pressure of the pressure sensor in the state corresponding to the horizontal axis. Here, the gear pump is turned on, the drive gear pump is driven state, the solenoid valve is ON state, an open state in which the electromagnetic valve is opened, the off state, in the closed state are closed solenoid valve is there.

  In the dispensing flow path system, the factors causing abnormal internal wash water pressure values include forgetting to open the cock provided at the outlet of the water tank, deterioration of the gear pump, malfunction of the solenoid valve not closing, and loosening of the pipe connection. , Etc. are conceivable. The output value of the pressure sensor itself varies depending on the type of pressure sensor, the position of the pressure sensor, the piping dimensions, and the gear pump output adjustment pressure. The pressure sensor is a gauge pressure type. For example, the back pressure side is open to the atmosphere so that it is not affected by atmospheric pressure, and the pressure sensor can measure up to several hundred kPa.

  The pressure sensor output value in the vicinity of the dispensing nozzle shows 0 kPa (standby state) when no internal washing, suction, or discharge is performed. During the internal washing operation, the gear pump is turned on and the solenoid valve is also turned on, which normally increases to 250 kPa. In the case of other mechanism operation, the gear pump is on and the solenoid valve is off, and the pressure rises to several tens of kPa.

  When the water tank cock is closed or when the gear pump is deteriorated, the pressure value when the gear pump is turned on for the internal washing operation and the solenoid valve is turned on significantly decreases from the normal value (two-dot chain line) And dotted lines). In addition, when a malfunction occurs in which the solenoid valve does not fully close, the pressure value when the gear pump is on and the solenoid valve is off increases significantly with respect to the normal value (dashed line). Therefore, it is assumed that the respective monitoring is performed according to the ON / OFF timing of the gear pump and the solenoid valve.

  Next, as a pressure value regarded as abnormal, the internal washing pressure when the water supply tank cock is closed can only rise to about 70 kPa. On the other hand, the pressure value that the gear pump is considered to be deteriorated is around 200 kPa, and there is a large difference with respect to the normal value.

  Also, when cleaning the water supply tank, a maintenance command is input to the automatic analyzer to drain the water in the water tank, so set a flag at this time and monitor that it is less than 70 kPa. Therefore, it can be determined that it is highly possible that forgetting to open the feed water tank outlet cock is the cause of the internal washing pressure drop.

  Specifically, the control unit 6 monitors the on / off state of the gear pump and the electromagnetic valve and the pressure value of the pressure sensor, and makes the following determination.

When the gear pump is in the drive state and the solenoid valve is in the closed state, if the pressure value of the pressure sensor is equal to or greater than the predetermined first threshold, it is determined that the solenoid valve is malfunctioning or the gear pump Is in the drive state and the solenoid valve is open, and if the pressure value of the pressure sensor is less than the predetermined second threshold, it is determined that the gear pump or the water tank is abnormal.

Further, when the gear pump is in the drive state and the solenoid valve is in the open state, if the pressure value of the pressure sensor is less than the predetermined second threshold, it is not possible to determine whether the gear pump is deteriorated or the water tank is abnormal. Therefore, it is desirable that the control unit 6 make the following determination. A third threshold value (pressure value between the second chain line and the dotted line) lower than the second threshold value is set. If the third threshold value is equal to or higher than the third threshold value, the gear pump is deteriorated . If it is less than the threshold value, it is determined that the cock provided at the outlet of the water supply tank is in an abnormal state in the closed state.

  It has also been found that the pressure of the gear pump decreases depending on the usage time. Therefore, it is possible to preventive maintenance by setting a third threshold value for the pressure value just before the use prohibition pressure region and outputting an alarm when the pressure value falls below this threshold value.

  Further, when pressure sensors are provided in a plurality of flow paths, all pressure sensors are affected if the gear pump is deteriorated, so that it is possible to distinguish between abnormal flow paths and gear pump deterioration. Specifically, the dispensing nozzle has a plurality of dispensing nozzles, each dispensing nozzle has a pressure sensor, and each pressure sensor has a common gear pump, and the pressure sensor outputs are compared with each other. By doing so, it is possible to determine whether there is a flow path abnormality of each dispensing nozzle or a gear pump deterioration. By comparing each other, it is understood that the pressure value of the specific dispensing nozzle is extremely high, the value is extremely low, or the like is a flow path abnormality of the specific dispensing nozzle. On the other hand, when the pressure values of the plurality of pressure sensors are extremely higher or lower than normal, it can be determined that the gear pump is deteriorated in common. In addition, about the comparison of the output of this pressure sensor, it can be performed as detection of dispensing probe abnormality separately from abnormality detection of any of a solenoid valve, a gear pump, and a water supply tank.

  Further, when washing the dispensing nozzle, washing water is ejected around the nozzle, and unnecessary washing water droplets are removed by vacuum suction around the nozzle. Whether or not this vacuum suction is normally performed can be determined by the pressure sensor. In other words, when a mechanism for vacuum suction around the dispensing nozzle is provided, the control unit measures the output of the pressure sensor during vacuum suction and determines whether vacuum suction is normally performed based on the output. It can also be determined.

  Also, during the operation called the outer washing operation that cleans the periphery of the nozzle, the washing water flows even though it flows to the nozzle tip.Therefore, the suction operation is performed in the nozzle at this timing, and the pressure sensor output in the nozzle is It is also possible to measure and determine whether the wash water is flowing normally based on the output. In other words, when a mechanism is provided for cleaning the dispensing nozzle by spraying washing water around the dispensing nozzle, the dispensing syringe performs a suction operation of the ejected washing water during the cleaning. It is also possible to measure the output of the pressure sensor and determine whether the washing water is normally ejected based on the output.

  About discrimination | determination of these vacuum suction and washing water ejection, a threshold value is provided in the pressure value of a pressure sensor, and a control part can discriminate | determine automatically on the basis of this provided threshold value.

1 Sample probe
2 tubes
3 Syringe for dispensing
3a Cylinder 3b Plunger 4 Syringe drive mechanism for dispensing
5 Sample probe drive mechanism
6 Control unit 7 Sample container 8 Sample
9 Sample discharge position
10 Cleaning position
11 Water supply pump
12 Water tank
13 Washing water
14 Solenoid valve
15 Pressure sensor
16 branch block
17 Circuit
18 Gear pump
19 Water tank open / close cock

Claims (6)

A dispensing nozzle for sucking and discharging a predetermined amount of liquid;
A syringe for dispensing that changes the pressure in the dispensing nozzle;
A pressure sensor provided in a pipe connecting the dispensing nozzle and the dispensing syringe;
A solenoid valve for separating the liquid flow path in the pipe;
A water supply pump for supplying water to the dispensing nozzle;
A water supply tank for supplying water to the water supply pump;
A gear pump for applying water pressure to the liquid in the dispensing nozzle;
In an automatic analyzer equipped with
An automatic analyzer comprising: a mechanism for measuring an output of the pressure sensor and detecting an abnormality of the electromagnetic valve, the gear pump, or the water tank based on the output. The automatic analyzer according to claim 1, wherein
When the gear pump is in a driving state and the solenoid valve is in a closed state, if the pressure value of the pressure sensor is equal to or greater than a predetermined first threshold value, whether the solenoid valve is malfunctioning or not Or
When the gear pump is in the drive state and the solenoid valve is in the open state, if the pressure value of the pressure sensor is less than a predetermined second threshold, it is determined that the gear pump or the water tank is abnormal. An automatic analyzer characterized by that. The automatic analyzer according to claim 2,
Further, when the gear pump is in a driving state and the electromagnetic valve is in an open state, the pressure value of the pressure sensor is less than a predetermined second threshold value ,
If it is Jo Tokoro third threshold or more is a deterioration of the gear pump, before when Kisho less than the constant third threshold, cock closed state provided at the outlet of the water tank An automatic analyzer characterized by determining that it is an abnormality. The automatic analyzer according to claim 1, wherein
The dispensing nozzle includes a plurality of dispensing nozzles, each dispensing nozzle includes the pressure sensor, and each pressure sensor includes the common gear pump,
An automatic analyzer characterized by comparing the output of the pressure sensor with each other to determine whether the flow path of each dispensing nozzle is abnormal or the gear pump is deteriorated. The automatic analyzer according to claim 1, wherein
In addition, a mechanism for vacuum suction around the dispensing nozzle,
An automatic analyzer that measures an output of the pressure sensor during the vacuum suction and determines whether the vacuum suction is normally performed based on the output. The automatic analyzer according to claim 1, wherein
Furthermore, a mechanism for jetting washing water around the dispensing nozzle to wash the dispensing nozzle is provided,
During the washing, the dispensing syringe performs a suction operation of the jetted washing water, measures the output of the pressure sensor during the washing, and based on the output, the washing water is ejected normally. An automatic analyzer characterized by determining whether or not