JP5829216B2 - 微生物農薬組成物 - Google Patents
微生物農薬組成物 Download PDFInfo
- Publication number
- JP5829216B2 JP5829216B2 JP2012542933A JP2012542933A JP5829216B2 JP 5829216 B2 JP5829216 B2 JP 5829216B2 JP 2012542933 A JP2012542933 A JP 2012542933A JP 2012542933 A JP2012542933 A JP 2012542933A JP 5829216 B2 JP5829216 B2 JP 5829216B2
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- JP
- Japan
- Prior art keywords
- pesticide composition
- bacillus
- microbial pesticide
- hydroxide
- inner core
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 239000000203 mixture Substances 0.000 title claims description 159
- 239000000575 pesticide Substances 0.000 title claims description 62
- 230000000813 microbial effect Effects 0.000 title claims description 59
- -1 alkylbenzene sulfonate Chemical class 0.000 claims description 142
- 239000002245 particle Substances 0.000 claims description 52
- 244000063299 Bacillus subtilis Species 0.000 claims description 31
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 31
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 claims description 30
- JJLJMEJHUUYSSY-UHFFFAOYSA-L Copper hydroxide Chemical compound [OH-].[OH-].[Cu+2] JJLJMEJHUUYSSY-UHFFFAOYSA-L 0.000 claims description 29
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims description 28
- 239000008187 granular material Substances 0.000 claims description 27
- 238000000034 method Methods 0.000 claims description 23
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 18
- 238000002156 mixing Methods 0.000 claims description 14
- AXZKOIWUVFPNLO-UHFFFAOYSA-N magnesium;oxygen(2-) Chemical compound [O-2].[Mg+2] AXZKOIWUVFPNLO-UHFFFAOYSA-N 0.000 claims description 13
- 239000000395 magnesium oxide Substances 0.000 claims description 12
- CPLXHLVBOLITMK-UHFFFAOYSA-N magnesium oxide Inorganic materials [Mg]=O CPLXHLVBOLITMK-UHFFFAOYSA-N 0.000 claims description 12
- 230000000694 effects Effects 0.000 claims description 11
- 239000010419 fine particle Substances 0.000 claims description 10
- VTHJTEIRLNZDEV-UHFFFAOYSA-L magnesium dihydroxide Chemical compound [OH-].[OH-].[Mg+2] VTHJTEIRLNZDEV-UHFFFAOYSA-L 0.000 claims description 8
- 239000000347 magnesium hydroxide Substances 0.000 claims description 8
- 229910001862 magnesium hydroxide Inorganic materials 0.000 claims description 8
- PSZYNBSKGUBXEH-UHFFFAOYSA-M naphthalene-1-sulfonate Chemical compound C1=CC=C2C(S(=O)(=O)[O-])=CC=CC2=C1 PSZYNBSKGUBXEH-UHFFFAOYSA-M 0.000 claims description 7
- TWNQGVIAIRXVLR-UHFFFAOYSA-N oxo(oxoalumanyloxy)alumane Chemical compound O=[Al]O[Al]=O TWNQGVIAIRXVLR-UHFFFAOYSA-N 0.000 claims description 7
- 229910052782 aluminium Inorganic materials 0.000 claims description 6
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims description 6
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- ULUAUXLGCMPNKK-UHFFFAOYSA-N Sulfobutanedioic acid Chemical compound OC(=O)CC(C(O)=O)S(O)(=O)=O ULUAUXLGCMPNKK-UHFFFAOYSA-N 0.000 claims description 4
- 239000003945 anionic surfactant Substances 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical compound [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 claims description 3
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- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 26
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- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 16
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- CIFWZNRJIBNXRE-UHFFFAOYSA-N mepanipyrim Chemical compound CC#CC1=CC(C)=NC(NC=2C=CC=CC=2)=N1 CIFWZNRJIBNXRE-UHFFFAOYSA-N 0.000 description 12
- YEBIHIICWDDQOL-YBHNRIQQSA-N polyoxin Polymers O[C@@H]1[C@H](O)[C@@H](C(C=O)N)O[C@H]1N1C(=O)NC(=O)C(C(O)=O)=C1 YEBIHIICWDDQOL-YBHNRIQQSA-N 0.000 description 12
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- HIEHAIZHJZLEPQ-UHFFFAOYSA-M sodium;naphthalene-1-sulfonate Chemical compound [Na+].C1=CC=C2C(S(=O)(=O)[O-])=CC=CC2=C1 HIEHAIZHJZLEPQ-UHFFFAOYSA-M 0.000 description 8
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- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 6
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- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 6
- 150000001412 amines Chemical class 0.000 description 6
- MWKFXSUHUHTGQN-UHFFFAOYSA-N decan-1-ol Chemical compound CCCCCCCCCCO MWKFXSUHUHTGQN-UHFFFAOYSA-N 0.000 description 6
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- QAHFOPIILNICLA-UHFFFAOYSA-N Diphenamid Chemical compound C=1C=CC=CC=1C(C(=O)N(C)C)C1=CC=CC=C1 QAHFOPIILNICLA-UHFFFAOYSA-N 0.000 description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 5
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- IBSREHMXUMOFBB-JFUDTMANSA-N 5u8924t11h Chemical compound O1[C@@H](C)[C@H](O)[C@@H](OC)C[C@@H]1O[C@@H]1[C@@H](OC)C[C@H](O[C@@H]2C(=C/C[C@@H]3C[C@@H](C[C@@]4(O3)C=C[C@H](C)[C@@H](C(C)C)O4)OC(=O)[C@@H]3C=C(C)[C@@H](O)[C@H]4OC\C([C@@]34O)=C/C=C/[C@@H]2C)/C)O[C@H]1C.C1=C[C@H](C)[C@@H]([C@@H](C)CC)O[C@]11O[C@H](C\C=C(C)\[C@@H](O[C@@H]2O[C@@H](C)[C@H](O[C@@H]3O[C@@H](C)[C@H](O)[C@@H](OC)C3)[C@@H](OC)C2)[C@@H](C)\C=C\C=C/2[C@]3([C@H](C(=O)O4)C=C(C)[C@@H](O)[C@H]3OC\2)O)C[C@H]4C1 IBSREHMXUMOFBB-JFUDTMANSA-N 0.000 description 4
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- YDEXUEFDPVHGHE-GGMCWBHBSA-L disodium;(2r)-3-(2-hydroxy-3-methoxyphenyl)-2-[2-methoxy-4-(3-sulfonatopropyl)phenoxy]propane-1-sulfonate Chemical compound [Na+].[Na+].COC1=CC=CC(C[C@H](CS([O-])(=O)=O)OC=2C(=CC(CCCS([O-])(=O)=O)=CC=2)OC)=C1O YDEXUEFDPVHGHE-GGMCWBHBSA-L 0.000 description 4
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- AMFGTOFWMRQMEM-UHFFFAOYSA-N triazophos Chemical compound N1=C(OP(=S)(OCC)OCC)N=CN1C1=CC=CC=C1 AMFGTOFWMRQMEM-UHFFFAOYSA-N 0.000 description 1
- IQGKIPDJXCAMSM-UHFFFAOYSA-N triazoxide Chemical compound N=1C2=CC=C(Cl)C=C2[N+]([O-])=NC=1N1C=CN=C1 IQGKIPDJXCAMSM-UHFFFAOYSA-N 0.000 description 1
- YMXOXAPKZDWXLY-QWRGUYRKSA-N tribenuron methyl Chemical group COC(=O)[C@H]1CCCC[C@@H]1S(=O)(=O)NC(=O)N(C)C1=NC(C)=NC(OC)=N1 YMXOXAPKZDWXLY-QWRGUYRKSA-N 0.000 description 1
- REEQLXCGVXDJSQ-UHFFFAOYSA-N trichlopyr Chemical compound OC(=O)COC1=NC(Cl)=C(Cl)C=C1Cl REEQLXCGVXDJSQ-UHFFFAOYSA-N 0.000 description 1
- NFACJZMKEDPNKN-UHFFFAOYSA-N trichlorfon Chemical compound COP(=O)(OC)C(O)C(Cl)(Cl)Cl NFACJZMKEDPNKN-UHFFFAOYSA-N 0.000 description 1
- DQJCHOQLCLEDLL-UHFFFAOYSA-N tricyclazole Chemical compound CC1=CC=CC2=C1N1C=NN=C1S2 DQJCHOQLCLEDLL-UHFFFAOYSA-N 0.000 description 1
- XAIPTRIXGHTTNT-UHFFFAOYSA-N triflumuron Chemical compound C1=CC(OC(F)(F)F)=CC=C1NC(=O)NC(=O)C1=CC=CC=C1Cl XAIPTRIXGHTTNT-UHFFFAOYSA-N 0.000 description 1
- ZSDSQXJSNMTJDA-UHFFFAOYSA-N trifluralin Chemical compound CCCN(CCC)C1=C([N+]([O-])=O)C=C(C(F)(F)F)C=C1[N+]([O-])=O ZSDSQXJSNMTJDA-UHFFFAOYSA-N 0.000 description 1
- RVKCCVTVZORVGD-UHFFFAOYSA-N trinexapac-ethyl Chemical group O=C1CC(C(=O)OCC)CC(=O)C1=C(O)C1CC1 RVKCCVTVZORVGD-UHFFFAOYSA-N 0.000 description 1
- NRHFWOJROOQKBK-UHFFFAOYSA-N triphenyltin;hydrate Chemical compound O.C1=CC=CC=C1[Sn](C=1C=CC=CC=1)C1=CC=CC=C1 NRHFWOJROOQKBK-UHFFFAOYSA-N 0.000 description 1
- KVEQCVKVIFQSGC-UHFFFAOYSA-N tritosulfuron Chemical compound FC(F)(F)C1=NC(OC)=NC(NC(=O)NS(=O)(=O)C=2C(=CC=CC=2)C(F)(F)F)=N1 KVEQCVKVIFQSGC-UHFFFAOYSA-N 0.000 description 1
- WFKWXMTUELFFGS-UHFFFAOYSA-N tungsten Chemical compound [W] WFKWXMTUELFFGS-UHFFFAOYSA-N 0.000 description 1
- 229910052721 tungsten Inorganic materials 0.000 description 1
- 239000010937 tungsten Substances 0.000 description 1
- 229910052720 vanadium Inorganic materials 0.000 description 1
- LEONUFNNVUYDNQ-UHFFFAOYSA-N vanadium atom Chemical compound [V] LEONUFNNVUYDNQ-UHFFFAOYSA-N 0.000 description 1
- 239000010455 vermiculite Substances 0.000 description 1
- 235000019354 vermiculite Nutrition 0.000 description 1
- 229910052902 vermiculite Inorganic materials 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 239000001993 wax Substances 0.000 description 1
- 239000000230 xanthan gum Substances 0.000 description 1
- 229920001285 xanthan gum Polymers 0.000 description 1
- 235000010493 xanthan gum Nutrition 0.000 description 1
- 229940082509 xanthan gum Drugs 0.000 description 1
- WCJYTPVNMWIZCG-UHFFFAOYSA-N xylylcarb Chemical compound CNC(=O)OC1=CC=C(C)C(C)=C1 WCJYTPVNMWIZCG-UHFFFAOYSA-N 0.000 description 1
- 239000005943 zeta-Cypermethrin Substances 0.000 description 1
- DUBNHZYBDBBJHD-UHFFFAOYSA-L ziram Chemical compound [Zn+2].CN(C)C([S-])=S.CN(C)C([S-])=S DUBNHZYBDBBJHD-UHFFFAOYSA-L 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/04—Preserving or maintaining viable microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Virology (AREA)
- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Inorganic Chemistry (AREA)
- Toxicology (AREA)
Description
(1)バシルス・サチリス(Bacillus subtilis)及び/又はバシルス・アミロリケファシエンス(Bacillus amyloliquefaciens)の生芽胞と金属酸化物、金属水酸化物、金属酸化物ケイ酸塩、金属水酸化物ケイ酸塩から選択される少なくとも1種以上を配合してなる微生物農薬組成物
(2)バシルス・サチリス(Bacillus subtilis)及び/又はバシルス・アミロリケファシエンス(Bacillus amyloliquefaciens)の生芽胞を含有する微粒体、あるいは粒状物を内核とし、当該内核の表面を金属酸化物、金属水酸化物、金属酸化物ケイ酸塩、金属水酸化物ケイ酸塩から選択される1種以上で被覆してなる(1)に記載の微生物農薬組成物。
(3)バシルス・サチリス(Bacillus subtilis)及び/又はバシルス・アミロリケファシエンス(Bacillus amyloliquefaciens)の生芽胞を含有する微粒体の平均粒径が0.05〜5mmであり、金属酸化物、金属水酸化物、金属酸化物ケイ酸塩、金属水酸化物ケイ酸塩から選択される1種以上の体積中位径が0.01〜250μmである(2)に記載の微生物農薬組成物。
(4)バシルス・サチリス(Bacillus subtilis)及び/又はバシルス・アミロリケファシエンス(Bacillus amyloliquefaciens)の生芽胞を含有する微粒体の平均粒径と当該内核表面を被覆する金属酸化物、金属水酸化物、金属酸化物ケイ酸塩、金属水酸化物ケイ酸塩から選択される1種以上の体積中位径の比(微粒体平均粒径/金属酸化物、あるいは金属水酸化物の体積中位径)が、5〜750である(3)に記載の微生物農薬組成物。
(5)金属酸化物が亜鉛、アルミニウム、鉄、チタン、マグネシウムの酸化物から選択される少なくとも1種以上、金属水酸化物がアルミニウム、鉄、マグネシウム、銅の水酸化物から選択される少なくとも1種以上、金属酸化物ケイ酸塩、あるいは金属水酸化物のケイ酸塩が酸化アルミニウムケイ酸塩である(1)〜(4)のいずれか1つに記載の微生物農薬組成物。
(6)更に、界面活性剤を配合してなる(1)〜(5)のいずれか1つに記載の微生物農薬組成物。
(7)界面活性剤を内核に配合してなり、かつ金属酸化物、金属水酸化物、金属酸化物ケイ酸塩、金属水酸化物ケイ酸塩から選択される少なくとも1種以上とともに内核表面にも被覆してなる(6)に記載の微生物農薬組成物。
(8)界面活性剤を内核のみに配合してなる(6)に記載の微生物農薬組成物。
(9)界面活性剤を金属酸化物、金属水酸化物、金属酸化物ケイ酸塩、金属水酸化物ケイ酸塩から選択される少なくとも1種以上とともに内核表面に被覆のみしてなる(内核には界面活性剤は含まれない)(6)に記載の微生物農薬組成物。
(10)界面活性剤がアニオン性界面活性剤である(6)〜(9)のいずれか1つに記載の微生物農薬組成物。
(11)界面活性剤がリグニンスルホン酸塩、アルキルベンゼンスルホン酸塩、ナフタレンスルホン酸塩、及びジアルキルスルホサクシネート塩の中から選択される少なくとも1種以上である(10)に記載の微生物農薬組成物。
(12)組成物全質量における水分含量が2.5質量%未満(好ましくは、1.5質量%未満)であることを特徴とする、(1)〜(11)のいずれか1つに記載の微生物農薬組成物。
(13)バシルス・サチリス(Bacillus subtilis)及び/又はバシルス・アミロリケファシエンス(Bacillus amyloliquefaciens)の生芽胞を含有する微粒体、あるいは粒状物を内核とし、当該内核の表面を金属酸化物、金属水酸化物、金属酸化物ケイ酸塩、金属水酸化物ケイ酸塩から選択される少なくとも1種以上で被覆することを特徴とする、微生物農薬組成物中の該芽胞を長期間安定し、生存した状態で保存する方法。
形態:桿菌
大きさ:幅1.0〜1.2μm、長さ3〜5μm
運動性:+
鞭毛の着生状態:周鞭毛
内生胞子:+
胞子の位置:中央
胞子の膨張:−
コロニーの色:白色〜薄い茶色
肉汁寒天平板培養:白色〜クリーム色のコロニーを形成し、表面はしわ状
グラム染色性:+
硝酸塩の還元:+
MR試験:−
VP試験:+
インドールの生成:−
澱粉の加水分解:+
クエン酸の資化性:+
無機窒素源:+
オキシダーゼ:−
カタラーゼ:+
生育pH
6.8、肉エキス培地:+
5.7、肉エキス培地:+
生育温度
30℃:+
50℃:−
生育NaCl濃度
2%:+
5%:+
7%:+
好気的生育:+
嫌気的生育:−
O−Fテスト:O
卵黄反応:−
グルコースからの酸生成:+
マンニトールからの酸生成:−
L−アラビノースからの酸生成:−
D−キシロースからの酸生成:−
グルコースからのガス生成:−
β−ガラクトシダーゼ:−
NaCl及びKCl要求性:−
(methoxyfenozide)、メトトリン(methothrin)、メトフルトリン(metofluthrin)、メトプレン(methoprene)、メトルカルブ(metholcarb)、メビンホス(mevinphos)、モノクロトホス(monocrotophos)、ラムダシハロトリン(lambda−cyhalothrin)、ルフェヌロン(lufenuron)、レスメトリン(resmethrin)、レピメクチン(lepmectin)、プロピレングリコールモノ脂肪酸エステル(propylene glycol monolaurate)、硫酸ニコチン(nicotine sulfate)、塩酸レバミゾール(levamisol)、酸化エチレン(ethylene oxide)、酸化フェンブタスズ(fenbutatin oxide)、脂肪酸グリセリド、酒石酸モランテル、なたね油、デンプン、大豆レシチン、BT剤、バーティシリウム・レカニ、パスツーリア・ペネトランス、スタイナーネマ・カーポカプサエ、ペキロマイセス・フモソロセウスより選ばれるものが使用できる。ここでいうBT剤とは細菌のバシルス・チューリンゲンシス(Bacillus thuringiensis)を利用した農薬の総称であり、菌の産生する結晶タンパク、生菌胞子、その両方の混合物があるが、本発明ではそのいずれも用いることができる。
ル(flamprop−M−methyl)、フラムプロップエチル(flamprop−ethyl)、フラムプロップイソプロピル(flamprop−isopropyl)、フラムプロップMイソプロピル(flamprop−M−isopropyl)、プリミスルフロン(primisulfuron−methyl)、フルアジホップ(fluazifop−butyl)、フルアジホップP(fluazifop−P−butyl)、フルアゾレート(fluazolate)、フルオメツロン(fluometuron)、フルオログリコフェン(fluoroglycofen−ethyl)、フルカルバゾンナトリウム塩(flucarbazone−sodium)、フルセトスルフロン(flucetosulfuron)、フルチアセットメチル(fluthiacet−methyl)、フルピルスルフロン(flupyrsulfuron−methyl−sodium)、フルフェナセット(flufenacet)、フルフェンピルエチル(flufenpyr−ethyl)、フルプロパネートナトリウム塩(flupropanate−sodium)、フルポキサム(flupoxam)、フルミオキサジン(flumioxazin)、フルミクロラックペンチル(flumiclorac−pentyl)、フルメツラム(flumetsulam)、フルリドン(fluridone)、フルルタモン(flurtamone)、フルロキシピル(fluroxypyr)、フルロクロリドン(flurochloridone)、プレチラクロール(pretilachlor)、プロジアミン(prodiamine)、プロスルフロン(prosulfuron)、プロスルホカルブ(prosulfocarb)、プロパキザホップ(propaquizafop)、プロパクロール(propachlor)、プロパジン系(propazine)、プロパニル(propanil)、プロピザミド(propyzamide)、プロピソクロール(propisochlor)、プロピリスルフロン(propyrisulfuron)、プロファム(propham)、プロフルアゾール(profluazol)、プロポキシカルバゾンナトリウム塩(propoxycarbazone−sodium)、プロホキシジム(profoxydim)、ブロマシル(bromacil)、プロメトリン(prometryn)、プロメトン(prometon)、ブロモキシニル(bromoxyni)(酪酸、オクタン酸又はヘプタン酸等とのエステル体を含む)、ブロモブチド(bromobutide)、フロラスラム(florasulam)、ヘキサジノン(hexazinone)、ベスロジン(benefin)、ペトキサミド(pethoxamid)、ベナゾリン(benazolin)、ペノキススラム(penoxsulam)、ベフルブタミド(beflubutamid)、ペブレート(pebulate)、ベンカルバゾン(bencarbazone)、ベンズフェンジゾン(benzfendizone)、ベンスリド(bensulide)、ベンスルフロンメチル(bensulfuron−methyl)、ベンゾビシクロン(benzobicyclon)、ベンゾフェナップ(benzofenap)、ベンタゾン(bentazone)(ナトリウム等との塩を含む)、ペンタノクロール(pentanochlor)、ベンチオカーブ(benthiocarb)、ペンディメタリン(pendimethalin)、ペントキサゾン(pentoxazone)、ベンフルラリン(benfluralin)、ベンフレセート(benfuresate)、ホサミンアンモニウム(fosamine−ammonium)、ホメサフェン(fomesafen)、メコプロップ(mecoprop−potassium)、メコプロップPカリウム塩(mecoprop−P−potassium)、メソスルフロンメチル(mesosulfuron−methyl)、メソトリオン(mesotrione)、メタザクロール(metazachlor)、メタベンズチアズロン(methabenzthiazuron)、メタゾスルフロン(metazosulfuron)、メタミトロン(metamitron)、メタミホップ(metamifop)、メチルダイムロン(methyldymron)、メトキスロン(metoxuron)、メトスラム(metosulam)、メトスルフロンメチル(metsulfuron−methyl)、メトブロムロン(metobromuron)、メトベンズロン(metobenzuron)、メトラクロール(metolachlor)、メトリブジン(metribuzin)、メピコート・クロリド(mepiquat−chloride)、メフェナセット(mefenacet)、モノリニュロン(monolinuron)、モリネート(molinate)、ヨードスルフロンメチルナトリウム塩(iodosulfulon−methyl−sodium)、ラクトフェン(lactofen)、リニュロン(linuron)、リムスルフロン(rimsulfuron)、レナシル(lenacil)、ザントモナス・キャンペストリス、ドレクスレラ・モノセラスより選ばれるものが使用できる。
水分活性(Aw)=平衡相対湿度/100
静岡県内の空気中より分離されたバシルス・エスピーD747株(FERM BP−8234)を平板培地上で培養し、分離したコロニーをフラスコに植菌し、20mlのブイヨン培地(肉エキス1%、ペプトン1%、塩化ナトリウム0.5%)で、27℃、120rpm、1日間振盪培養後、得られた培養液をグルコース1%、可溶性澱粉2%、ポリペプトン0.5%、乾燥酵母1%、脱脂大豆1%、KH2PO40.2%、塩化ナトリウム0.2%、炭酸カルシウム0.3%を含む、pH6.0の培地20Lに植菌し、27℃、120rpm、3日間振盪培養した後、遠心集菌(10,000×g、15分間)し、滅菌水中に懸濁し、培地成分を洗浄した。この操作を2回行い、湿重量約1kgの湿菌体(芽胞画分)を得た。この芽胞画分はバシルス・エスピーD747の芽胞を乾燥重量で50重量%含有するものである。
上記製造例1によって得られたバシルス・エスピーD747の芽胞画分1kgを蒸留水5Lに懸濁後、アルキルアリールスルホン酸塩1部、リグニンスルホン酸塩10部、硫酸アンモニウム65部、炭酸カルシウム15部を粉砕混合した粉体4.5kgを流動層造粒機中の温風60℃で流動させながら、芽胞画分希釈液を噴霧し、流動層造粒を行ない、乾燥芽胞の粉粒体混合物約5kgを得た。この乾燥芽胞はバシルス・エスピーD747の芽胞を1×1011cfu/g以上含むものであり、所望の粒度部分を篩別して供試する。
製造例2で得られたバシルス・エスピーD747株乾燥芽胞を篩分して得られた粉粒体混合物(平均粒径400μm)を40%、殺菌成分メパニピリムを20%、ナフタレンスルホン酸ホルマリン縮合物ナトリウム塩1.5%、ポリオキシエチレンアルキルエーテル1.5%、珪藻土26%、アルミノケイ酸塩(合成ゼオライトA−3;東ソー株式会社、体積中位径:16.5μm)10%、クレー1%を均一に混合粉砕したプレミックス(体積中位径:12.7μm)と混合して水和剤とした。粉粒体混合物の表面はプレミックスで被覆された状態となった。
製造例2で得られたバシルス・エスピーD747株乾燥芽胞を篩分して得られた粉粒体混合物(平均粒径600μm)を40%、殺菌成分メパニピリムを20%、ナフタレンスルホン酸ホルマリン縮合物ナトリウム塩1.5%、ポリオキシエチレンアルキルエーテル1.5%、硫酸アンモニウム26%、活性アルミナ(活性アルミナV−R−3;ユニオン昭和株式会社、体積中位径:12.7μm)5%、クレー6%を均一に混合粉砕したプレミックス(体積中位径14.8μm)と混合して水和剤とした。粉粒体混合物の表面はプレミックスで被覆された状態となった。
製造例2で得られたバシルス・エスピーD747株乾燥芽胞を篩分して得られた粉体部分(平均粒径100μm)を90%、活性アルミナ(活性アルミナV−R−3;体積中位径:12.7μm)10%を混合して水和剤とした。
製造例2で得られたバシルス・エスピーD747株乾燥芽胞を篩分して得られた粉体部分(平均粒径100μm)を90%、酸化マグネシウム10%(体積中位径:20.1μm)を混合して水和剤とした。
製造例2で得られたバシルス・エスピーD747株乾燥芽胞を篩分して得られた粉粒体混合物(平均粒径600μm)を90%、酸化マグネシウム10%(体積中位径:20.1μm)を混合して水和剤とした。粉粒体混合物の表面は酸化マグネシウムで被覆された状態となった。
製造例2で得られたバシルス・エスピーD747株乾燥芽胞を篩分して得られた粉粒体混合物(平均粒径600μm)を40%、殺菌成分ポリオキシン20%、アルキルアリールスルホン酸塩2%、酸化マグネシウム(体積中位径:20.1μm)5%、硫酸アンモニウム33%を均一に混合粉砕したプレミックス(体積中位径:13.9μm)と混合して水和剤とした。粉粒体混合物の表面はプレミックスで被覆された状態となった。
製造例2で得られたバシルス・エスピーD747株乾燥芽胞を篩分して得られた粉粒体混合物(平均粒径600μm)を35%と、水酸化第二銅(体積中位径:3.6μm)50%、ナフタレンスルホン酸ホルマリン縮合物ナトリウム塩1.0%、リグニンスルホン酸ナトリウム塩6.5%、硫酸アンモニウム7.5%を均一に混合粉砕したプレミックス(体積中位径:6.5μm)とを混合して水和剤とした。粉粒体混合物の表面はプレミックスで被覆された状態となった。
製造例2で得られたバシルス・エスピーD747株乾燥芽胞を篩分して得られた粉粒体混合物(平均粒径500μm)を35%と、水酸化第二銅(体積中位径:5.6μm)50%、ジアルキルナフタレンスルホン酸ナトリウム1.0%、リグニンスルホン酸ナトリウム塩6.5%、硫酸アンモニウム7.5%を均一に混合粉砕したプレミックス(体積中位径:7.6μm)とを混合して水和剤とした。粉粒体混合物の表面はプレミックスで被覆された状態となった。
製造例2で得られたバシルス・エスピーD747株乾燥芽胞を篩分して得られた粉粒体混合物(平均粒径600μm)を35%と、水酸化マグネシウム(体積中位径:5.7μm)10%、ジアルキルナフタレンスルホン酸ナトリウム1.0%、リグニンスルホン酸ナトリウム塩6.5%、硫酸アンモニウム40%、クレー7.5%を均一に混合粉砕したプレミックス(体積中位径:9.8μm)とを混合して水和剤とした。粉粒体混合物の表面はプレミックスで被覆された状態となった。
製造例2で得られたバシルス・エスピーD747株乾燥芽胞を篩分して得られた粉粒体混合物を篩分して得た粉体部分(平均粒径100μm)を35%と、水酸化第二銅(体積中位径:5.6μm)50%、ジアルキルナフタレンスルホン酸ナトリウム1.0%、リグニンスルホン酸ナトリウム塩6.5%、クレー7.5%を均一に混合粉砕したプレミックス(体積中位径:7.6μm)とを混合して水和剤とした。
製造例2で得られたバシルス・エスピーD747株乾燥芽胞を篩分して得られた粉体部分(平均粒径:125μm)を35%と、酸化マグネシウム(体積中位径:20.1μm)10%、ジアルキルナフタレンスルホン酸ナトリウム1.0%、リグニンスルホン酸ナトリウム塩6.5%、硫酸アンモニウム40.0%、クレー7.5%を均一に混合粉砕したプレミックス(体積中位径:24.2μm)とを混合して水和剤とした。
製造例2で得られたバシルス・エスピーD747株乾燥芽胞を篩分して得られた粉粒体混合物(平均粒径600μm)を40%、殺菌成分メパニピリムを20%、ナフタレンスルホン酸ホルマリン縮合物ナトリウム塩1.5%、ポリオキシエチレンアルキルエーテル1.5%、珪藻土26%、アルミノケイ酸塩(合成ゼオライトA−3;体積中位径:16.5μm)10%、クレー1%を均一に混合粉砕したプレミックス(体積中位径:12.7μm)と混合して水和剤とした。粉粒体混合物の表面はプレミックスで被覆された状態となった。
製造例2で得られたバシルス・エスピーD747株乾燥芽胞を篩分して得られた粉粒体混合物(平均粒径400μm)を40%、殺菌成分メパニピリムを20%、ナフタレンスルホン酸ホルマリン縮合物ナトリウム塩1.5%、ポリオキシエチレンアルキルエーテル1.5%、珪藻土26%、アルミノケイ酸塩(モレキュラーシーブ13X;ユニオン昭和株式会社、体積中位径:8.7μm)10%、クレー1%を均一に混合粉砕したプレミックス(体積中位径:10.0μm)と混合して水和剤とした。粉粒体芽胞液の表面はプレミックスで被覆された状態となった。
製造例2で得られたバシルス・エスピーD747株乾燥芽胞を篩分して得られた粉粒体混合物(平均粒径600μm)を40%、殺菌成分ポリオキシン20%、アルキルアリールスルホン酸塩2%、アルミノケイ酸塩(モレキュラーシーブ4A;ユニオン昭和株式会社、体積中位径:8.3μm)10%、硫酸アンモニウム28%を均一に混合粉砕したプレミックス(体積中位径:10.0μm)と混合して水和剤とした。粉粒体混合物の表面はプレミックスで被覆された状態となった。
製造例2で得られたバシルス・エスピーD747株乾燥芽胞を篩分して得られた粉粒体混合物(平均粒径600μm)を40%、殺菌成分ポリオキシン20%、アルキルアリールスルホン酸塩2%、アルミノケイ酸塩(モレキュラーシーブ13X;体積中位径:8.7μm)10%、硫酸アンモニウム28%を均一に混合粉砕したプレミックス(体積中位径:10.0μm)と混合して水和剤とした。
製造例2で得られたバシルス・エスピーD747株乾燥芽胞を篩分して得られた粉粒体混合物(平均粒径600μm)を40%、殺菌成分ポリオキシン20%、アルキルアリールスルホン酸塩2%、アルミノケイ酸塩(モレキュラーシーブ3A;ユニオン昭和株式会社、体積中位径:13.0μm)10%、硫酸アンモニウム28%を均一に混合粉砕したプレミックス(体積中位径:10.0μm)と混合して水和剤とした。粉粒体混合物の表面はプレミックスで被覆された状態となった。
製造例2で得られたバシルス・エスピーD747株乾燥芽胞を篩分して得られた粉体部分(平均粒径125μm)を40%と、殺菌成分ポリオキシン20%、アルキルアリールスルホン酸塩2%、アルミノケイ酸塩(モレキュラーシーブ3A)10%、硫酸アンモニウム28%を均一に混合粉砕したプレミックス(体積中位径:10.0μm)と混合して水和剤とした。
製造例2で得られたバシルスsp.D747菌株乾燥芽胞の粉粒体混合物を篩分して得た粉体部分(平均粒径125μm)を40%と、殺菌成分メパニピリム20%、ナフタレンスルホン酸ホルマリン縮合物ナトリウム塩1.5%、ポリオキシエチレンアルキルエーテル1.5%、アルミノケイ酸塩(合成ゼオライトA−3)10%、珪藻土26%、クレー1.0%を均一に混合粉砕したプレミックス(体積中位径:12.7μm)と混合して水和剤とした。
製造例2で得られたバシルス・エスピーD747株乾燥芽胞を篩分して得られた粉体部分(平均粒径125μm)を40%と、殺菌成分メパニピリム20%、ナフタレンスルホン酸ホルマリン縮合物ナトリウム塩1.5%、ポリオキシエチレンアルキルエーテル1.5%、アルミノケイ酸塩(モレキュラーシーブ13X)10%、珪藻土26%、クレー1.0%を均一に混合粉砕したプレミックス(体積中位径:10.0μm)と混合して水和剤とした。
製造例2で得られたバシルス・エスピーD747株乾燥芽胞を篩分して得られた粉体部分(平均粒径125μm)を40%と、殺菌成分ポリオキシン20%、アルキルアリールスルホン酸塩2%、活性アルミナ(活性アルミナV−R−3)5%、硫酸アンモニウム33%を均一に混合粉砕したプレミックス(体積中位径:14.0μm)と混合して水和剤とした。
製造例2で得られたバシルス・エスピーD747株乾燥芽胞を篩分して得られた粉粒体混合物(平均粒径400μm)を40%、殺菌成分ポリオキシン20%、アルキルアリールスルホン酸塩2%、アルミノケイ酸塩(モレキュラーシーブ13X)10%、硫酸アンモニウム28%を均一に混合粉砕したプレミックス(体積中位径:10.0μm)と混合して水和剤とした。粉粒体混合物の表面はプレミックスで被覆された状態となった。
製造例2で得られたバシルス・エスピーD747株乾燥芽胞を篩分して得られた粉体部分(平均粒径125μm)を40%と、殺菌成分ポリオキシン20%、アルキルアリールスルホン酸塩2%、アルミノケイ酸塩(モレキュラーシーブ13X)10%、硫酸アンモニウム28%を均一に混合粉砕したプレミックス(体積中位径:10.0μm)と混合して水和剤とした。
製造例2で得られたバシルス・エスピーD747株乾燥芽胞を篩分して得られた粉体部分(平均粒径125μm)を40%と、殺菌成分メパニピリム20%、ナフタレンスルホン酸ホルマリン縮合物ナトリウム塩1.5%、ポリオキシエチレンアルキルエーテル1.5%、活性アルミナ(活性アルミナV−R−3)5%、硫酸アンモニウム26%、クレー6.0%を均一に混合粉砕したプレミックス(体積中位径:14.8μm)と混合して水和剤とした。
製造例2で得られたバシルス・エスピーD747株乾燥芽胞を篩分して得られた粉粒体混合物(平均粒径250μm)を40%、殺菌成分ポリオキシン20%、アルキルアリールスルホン酸塩2%、アルミノケイ酸塩(合成ゼオライトA−3)10%、硫酸アンモニウム28%を均一に混合粉砕したプレミックス(体積中位径:12.7μm)と混合して水和剤とした。粉粒体混合物の表面はプレミックスで被覆された状態となった。
製造例2で得られたバシルス・エスピーD747株乾燥芽胞を篩分して得られた粉粒体混合物(平均粒径250μm)を40%、殺菌成分メパニピリムを20%、ナフタレンスルホン酸ホルマリン縮合物ナトリウム塩1.5%、ポリオキシエチレンアルキルエーテル1.5%、硫酸アンモニウム26%、活性アルミナ(活性アルミナV−R−3)5%、クレー6%を均一に混合粉砕したプレミックス(体積中位径14.8μm)と混合して水和剤とした。粉粒体混合物の表面はプレミックスで被覆された状態となった。
製造例2で得られたバシルス・エスピーD747株乾燥芽胞を篩分して得られた粉粒体混合物(平均粒径:250μm)を35%と、水酸化第二銅(体積中位径:5.6μm)50%、ジアルキルナフタレンスルホン酸ナトリウム1.0%、リグニンスルホン酸ナトリウム塩6.5%、硫酸アンモニウム7.5%を均一に混合粉砕したプレミックス(体積中位径:7.6μm)とを混合して水和剤とした。粉粒体混合物の表面はプレミックスで被覆された状態となった。
製造例2で得られたバシルス・エスピーD747株乾燥芽胞を篩分して得られた粉粒体混合物(平均粒径:400μm)を35%と、水酸化第二銅(体積中位径:5.6μm)50%、ジアルキルナフタレンスルホン酸ナトリウム1.0%、リグニンスルホン酸ナトリウム塩6.5%、硫酸アンモニウム7.5%を均一に混合粉砕したプレミックス(体積中位径:7.6μm)とを混合して水和剤とした。粉粒体混合物の表面はプレミックスで被覆された状態となった。
製造例2で得られたバシルス・エスピーD747株乾燥芽胞を篩分して得られた粉粒体混合物(平均粒径250μm)を40%、殺菌成分メパニピリムを20%、ナフタレンスルホン酸ホルマリン縮合物ナトリウム塩1.5%、ポリオキシエチレンアルキルエーテル1.5%、硫酸アンモニウム26%、アルミノケイ酸塩(合成ゼオライトA−3)10%、クレー1%を均一に混合粉砕したプレミックス(体積中位径12.7μm)と混合して水和剤とした。粉粒体混合物の表面はプレミックスで被覆された状態となった。
製造例2で得られたバシルス・エスピーD747株乾燥芽胞を篩分して得られた粉粒体混合物(平均粒径400μm)を40%、殺菌成分メパニピリムを20%、ナフタレンスルホン酸ホルマリン縮合物ナトリウム塩1.5%、ポリオキシエチレンアルキルエーテル1.5%、硫酸アンモニウム26%、活性アルミナ(活性アルミナV−R−3)5%、クレー6%を均一に混合粉砕したプレミックス(体積中位径14.8μm)と混合して水和剤とした。粉粒体混合物の表面はプレミックスで被覆された状態となった。
製造例2で得られたバシルス・エスピーD747株乾燥芽胞を篩分して得られた粉粒体混合物(平均粒径400μm)を40%、殺菌成分ポリオキシン20%、アルキルアリールスルホン酸塩2%、アルミノケイ酸塩(モレキュラーシーブ3A)10%、硫酸アンモニウム28%を均一に混合粉砕したプレミックス(体積中位径:10.0μm)と混合して水和剤とした。粉粒体混合物の表面はプレミックスで被覆された状態となった。
製造例2で得られたバシルス・エスピーD747株乾燥芽胞を篩分して得られた粉体部分(平均粒径100μm)を35%と、水酸化第二銅(体積中位径:5.6μm)50%、ジアルキルナフタレンスルホン酸ナトリウム1.0%、リグニンスルホン酸ナトリウム塩6.5%、硫酸アンモニウム7.5%を均一に混合粉砕したプレミックス(体積中位径:7.6μm)とを混合して水和剤とした。
製造例2で得られたバシルス・エスピーD747株乾燥芽胞を篩分して得られた粉粒体混合物(平均粒径600μm)を40%、殺菌成分メパニピリムを20%、ナフタレンスルホン酸ホルマリン縮合物ナトリウム塩1.5%、ポリオキシエチレンアルキルエーテル1.5%、珪藻土36%、クレー1%を均一に混合粉砕したプレミックス(体積中位径:13.7μm)と混合して水和剤とした。粉粒体芽胞液の表面はプレミックスで被覆された状態となった。
製造例2で得られたバシルス・エスピーD747株乾燥芽胞を篩分して得られた粉体混合物(平均粒径100μm)を40%、殺菌成分メパニピリムを20%、ナフタレンスルホン酸ホルマリン縮合物ナトリウム塩1.5%、ポリオキシエチレンアルキルエーテル1.5%、硫酸アンモニウム36%、クレー1%を均一に混合粉砕したプレミックス(体積中位径:16.0μm)と混合して水和剤とした。
製造例2で得られたバシルス・エスピーD747株乾燥芽胞を篩分して得られた粉粒体混合物(平均粒径600μm)をそのまま水和剤とした。
製造例2で得られたバシルス・エスピーD747株乾燥芽胞を篩分して得られた粉粒体混合物(平均粒径400μm)をそのまま水和剤とした。
製造例2で得られたバシルス・エスピーD747株乾燥芽胞を篩分して得られた粉体混合物(平均粒径100μm)をそのまま水和剤とした。
製造例2で得られたバシルス・エスピーD747株乾燥芽胞を篩分して得られた粉粒体混合物(平均粒径600μm)を40%と、殺菌成分ポリオキシン20%、アルキルアリールスルホン酸塩2%、珪藻土38%を均一に混合粉砕したプレミックス(体積中位径:11.9μm)と混合して水和剤とした。粉粒体混合物の表面はプレミックスで被覆された状態となった。
製造例2で得られたバシルス・エスピーD747株乾燥芽胞を篩分して得られた粉体混合物(平均粒径100μm)を40%と、殺菌成分ポリオキシン20%、アルキルアリールスルホン酸塩2%、硫酸アンモニウム38%を均一に混合粉砕したプレミックス(体積中位径:13.8μm)と混合して水和剤とした。
製剤例、比較製剤例で試作した試料の水分をカールフィッシャー法で測定した。
製剤例、比較製剤例で試作した試料の水分活性を500mlの密封容器内に試料10gを入れて密封し、20℃における密封容器内の平衡相対湿度を測定することにより求めた。水分活性は平衡相対湿度より次式で算出される。
水分活性(Aw)=平衡相対湿度/100
製剤例、比較製剤例で試作した試料の密封包装袋を54℃に14日放置した。また空調の無い倉庫(室温)に3年間放置した。初期生菌数と各放置後生菌数から生菌数減少率を算出し、表1に表した。生菌数は、普通寒天培地を用い、希釈平板法にて生菌数を測定した。培地は27℃で48時間培養を行い、コロニーを形成させ、コロニー形成数から生菌数を推定した。これをコロニー形成ユニット(cfu)とし、製剤1g当りの生菌数とした。
すなわち本発明は、バシルス(Bacillus)属に属する細菌の生芽胞および化学農薬剤を含有する微生物農薬組成物中の該芽胞を長期間安定して、生存した状態で保存し得る組成物、および該芽胞を長期間安定して、生存した状態で保存する方法を提供することを目的とする。
Claims (11)
- バシルス・サチリス(Bacillus subtilis)及び/又はバシルス・アミロリケファシエンス(Bacillus amyloliquefaciens)の生芽胞を含有する微粒体、あるいは粒状物を内核とし、当該内核の表面をアルミノケイ酸塩、酸化アルミニウム、酸化マグネシウム、水酸化第二銅、水酸化マグネシウムから選択される1種以上で被覆してなり、20℃における水分活性が0.5未満である微生物農薬組成物。
- バシルス・サチリス(Bacillus subtilis)及び/又はバシルス・アミロリケファシエンス(Bacillus amyloliquefaciens)の生芽胞を含有する微粒体、あるいは粒状物の平均粒径が0.05〜5mmであり、アルミノケイ酸塩、酸化アルミニウム、酸化マグネシウム、水酸化第二銅、水酸化マグネシウムから選択される1種以上の体積中位径が0.01〜250μmである請求項1に記載の微生物農薬組成物。
- バシルス・サチリス(Bacillus subtilis)及び/又はバシルス・アミロリケファシエンス(Bacillus amyloliquefaciens)の生芽胞を含有する微粒体、あるいは粒状物の平均粒径と当該内核表面を被覆するアルミノケイ酸塩、酸化アルミニウム、酸化マグネシウム、水酸化第二銅、水酸化マグネシウムから選択される1種以上の体積中位径の比(微粒体、あるいは粒状物の平均粒径/アルミノケイ酸塩、酸化アルミニウム、酸化マグネシウム、水酸化第二銅、水酸化マグネシウムから選択される1種以上の体積中位径)が、5〜750である請求項2に記載の微生物農薬組成物。
- 更に、界面活性剤を配合してなる請求項1〜3のいずれか1項に記載の微生物農薬組成物。
- 界面活性剤を内核に配合してなり、かつアルミノケイ酸塩、酸化アルミニウム、酸化マグネシウム、水酸化第二銅、水酸化マグネシウムから選択される少なくとも1種以上とともに内核表面にも被覆してなる請求項4に記載の微生物農薬組成物。
- 界面活性剤を内核のみに配合してなる請求項4に記載の微生物農薬組成物。
- 界面活性剤をアルミノケイ酸塩、酸化アルミニウム、酸化マグネシウム、水酸化第二銅、水酸化マグネシウムから選択される少なくとも1種以上とともに内核表面に被覆のみしてなる請求項4に記載の微生物農薬組成物。
- 界面活性剤がアニオン性界面活性剤である請求項4〜7のいずれか1項に記載の微生物農薬組成物。
- 界面活性剤がリグニンスルホン酸塩、アルキルベンゼンスルホン酸塩、ナフタレンスルホン酸塩、及びジアルキルスルホサクシネート塩の中から選択される少なくとも1種以上である請求項8に記載の微生物農薬組成物。
- 組成物全質量における水分含量が2.5質量%未満であることを特徴とする、請求項1〜9のいずれか1項に記載の微生物農薬組成物。
- バシルス・サチリス(Bacillus subtilis)及び/又はバシルス・アミロリケファシエンス(Bacillus amyloliquefaciens)の生芽胞を含有する微粒体、あるいは粒状物を内核とし、当該内核の表面をアルミノケイ酸塩、酸化アルミニウム、酸化マグネシウム、水酸化第二銅、水酸化マグネシウムから選択される少なくとも1種以上で被覆し、20℃における水分活性が0.5未満とすることを特徴とする、微生物農薬組成物中の該芽胞を長期間安定し、生存した状態で保存する方法。
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