JP5820622B2 - Fermented soymilk and method for producing the same - Google Patents

Description

  The present invention relates to a fermented soymilk and a method for producing the same.

  In recent years, fermented soymilk obtained by fermenting soymilk with lactic acid has attracted attention as a new food or food material with low cholesterol and low calories (see, for example, Patent Documents 1 and 2).

JP 2007-14303 A JP 2008-161070 A

  Although some soymilk fermented products are known, the options are not many. In particular, the demand for fermented soymilk having a new texture is considered high.

  Then, this invention aims at providing the novel method which enables manufacture of the soymilk fermented product which has the original texture which is not in the conventional fermented soymilk product, and such a soymilk fermented product.

  The present invention provides a method for producing a fermented soymilk product comprising a step of fermenting soymilk using a lactic acid bacterium having an ability to produce β-glucan (hereinafter sometimes referred to as “β-glucan-producing lactic acid bacteria”). In the present invention, “β-glucan production” means that lactic acid bacteria produce β-glucan outside the cells.

In the method of the present invention, β-glucan is produced outside the cells by lactic acid bacteria during fermentation. Therefore, a fermented soymilk containing β-glucan derived from lactic acid bacteria and having unique properties and texture can be obtained. According to the method of the present invention, for example, a soymilk fermented product having the following properties can be obtained.
-There is almost no separation of whey, and it is viscous and shows stringiness.
・ It is hard to collapse when put in the mouth and keeps smooth in the mouth until swallowed.

  As the β-glucan-producing lactic acid bacterium, those further having the ability to convert arginine into ornithine and / or citrulline are preferable. By performing fermentation of soymilk using such lactic acid bacteria, at least a part of arginine (which may be added to soymilk) in soymilk is converted to ornithine and / or citrulline during the fermentation process, and ornithine and / or Or it becomes possible to obtain the fermented soybean milk containing citrulline. In addition, ornithine is an amino acid that is contained in a large amount in shijimi and is known to have an effect of improving liver function. Citrulline is an amino acid that is abundant in cucurbitaceae plants (watermelon, cucumber, etc.) and is known to have a blood flow improving effect.

  Examples of β-glucan-producing lactic acid bacteria include Lactobacillus brevis SBC8027 (accession number: FERM BP-10630). Lactobacillus brevis SBC8027 is a lactic acid bacterium that further has the ability to convert arginine to ornithine and citrulline.

  The present invention also provides a fermented soymilk product obtainable by the above production method. The fermented soymilk of the present invention contains β-glucan derived from lactic acid bacteria and has a unique property and texture.

  In one aspect, the soymilk fermented product of the present invention is a fermented product having a viscosity of 4,000 to 10,000 mPa · s measured at 25 ° C. by a rotating cylindrical viscometer. Such a fermented soymilk has little separation of whey and is viscous and exhibits stringiness.

In one embodiment, the fermented soymilk of the present invention represents η ₅₀ / η ₁₀ [η ₁₀ , η ₅₀ represents the viscosity at shear rates of 10 s ⁻¹ and 50 s ⁻¹ measured at 25 ° C., respectively. ] Is a fermented product of 0.3 or more. Such a fermented soymilk is hard to collapse when put in the mouth, and remains smooth in the mouth until swallowed. The viscosities at shear rates of 10 s ⁻¹ and 50 s ⁻¹ correspond to the viscosity when put in the mouth and the viscosity when swallowed, respectively.

  The fermented soymilk of the present invention can be used as a food or food material. That is, the present invention also provides a food containing the fermented soymilk (may be composed of the fermented soymilk). The food of the present invention is a food to which the properties of the fermented soybean milk are appropriately given.

  ADVANTAGE OF THE INVENTION According to this invention, the novel method which enables manufacture of the soymilk fermented product which has the original texture which is not in the conventional soymilk fermented product, and such a soymilk fermented product is provided.

It is an alignment result of a known gtf gene region and a corresponding DNA fragment derived from Lactobacillus brevis SBC8027. It is an alignment result of a known gtf gene region and a corresponding DNA fragment derived from Lactobacillus brevis SBC8027. It is an optical microscope photograph which shows the presence or absence of aggregation of Lactobacillus brevis SBC8027 by the antibody process. (A) is a photograph before processing, and (b) is a photograph after processing. It is a gel filtration chromatogram about the exopolysaccharide in the culture of Lactobacillus brevis SBC8027. It is a graph which shows a monosaccharide composition about the exopolysaccharide (4 fractions) in the culture of Lactobacillus brevis SBC8027. It is a ¹ H-NMR spectrum of the exopolysaccharide of cultures of Lactobacillus brevis SBC8027 (1 fraction). It is a graph which shows the relationship between a shear rate and a viscosity about the soymilk fermented material manufactured using Lactobacillus brevis SBC8027. It is a graph which shows the relationship between a shear rate and a viscosity about the soymilk fermented material manufactured using Lactobacillus brevis SBC8027. It is a graph which shows the relationship between a shear rate and a viscosity about the soymilk fermented material manufactured using Lactobacillus brevis SBC8027.

  Embodiments of the present invention will be described below.

Method for producing fermented soymilk:
The method for producing a fermented soymilk product of the present invention is a method including a step of fermenting soymilk using a lactic acid bacterium having β-glucan-producing ability.

  In the method of the present invention, for example, 3% (w / w) or more (particularly 4 to 16% (w / w)) of soybean solids (soybean components excluding moisture (carbohydrate, lipid, protein, etc.)) The contained soymilk can be used. Commercially available soy milk may be used (in JAS (Japanese Agricultural Standards), soy milk beverage (others) has a solid content of 4% or more).

  The soy milk may be further added with sugar (sucrose, maltose, stachyose, raffinose, etc.), plant extract (eg, malt extract), flavor (eg, yogurt flavor), sweetener (eg, gum syrup), for example. .

  β-glucan-producing lactic acid bacteria include, for example, the genus Lactobacillus, the genus Bifidobacterium, the genus Enterococcus, the genus Streptococcus, the genus Lactococcus, Any bacterium of the genus Leuconostoc can be used.

  Examples of β-glucan producing lactic acid bacteria include Lactobacillus brevis SBC8027. The Lactobacillus brevis SBC8027 strain was founded on June 28, 2006 at the National Institute of Advanced Industrial Science and Technology, Patent Biological Depositary Center (1st, 1st East, 1-chome, Tsukuba, Ibaraki, Japan) (zip code 305-8565). It is a strain deposited under the accession number FERM BP-10630.

  Whether or not a certain lactic acid bacterium has β-glucan production ability can be determined, for example, as follows (see Examples). First, two types of primer sets corresponding to different regions on the β-glucan synthesis gene (gtf gene) (for example, a forward primer consisting of the base sequence of SEQ ID NO: 1 and a reverse primer set consisting of the base sequence of SEQ ID NO: 2) Using a forward primer consisting of the base sequence of SEQ ID NO: 3 and a reverse primer consisting of the base sequence of SEQ ID NO: 4), a polymerase chain reaction (PCR) is performed using the genomic DNA of the test lactic acid bacterium as a template. If both of the two DNA fragments are amplified, the test lactic acid bacterium is treated with an antibody that reacts with lactic acid β-glucan (for example, Streptococcus pneumoniae type 37-specific antiserum). If aggregation is observed, it is determined that the test lactic acid bacterium has β-glucan production ability. Genomic DNA extraction, PCR and the like can be performed by methods generally known to those skilled in the art.

  As the β-glucan-producing lactic acid bacterium, those further having the ability to convert arginine into ornithine and / or citrulline are preferable. Lactobacillus brevis SBC8027 is a lactic acid bacterium having the ability to convert arginine into ornithine and citrulline. When using such lactic acid bacteria, fermented soymilk containing more ornithine and / or citrulline can be obtained by adding arginine to soymilk and performing fermentation.

  Whether a certain lactic acid bacterium has the ability to convert arginine into ornithine and / or citrulline is, for example, fermenting soy milk (which may be supplemented with arginine) with the test lactic acid bacterium, and then arginine and ornithine in the fermented soy milk And by determining the amount of citrulline. If the amount of arginine decreases by fermentation and the amount of ornithine and / or citrulline increases, it is determined that the test lactic acid bacterium has the ability to convert arginine to ornithine and / or citrulline.

  In the fermentation, only β-glucan-producing lactic acid bacteria may be used, or β-glucan non-producing lactic acid bacteria may coexist.

Fermentation of soy milk can be performed based on a method for producing a fermented milk product (yogurt, lactic acid beverage, etc.) generally known to those skilled in the art, and the temperature and time during the fermentation are determined depending on the soy milk used. It can set suitably according to the kind of lactic acid bacteria. For example, β-glucan-producing lactic acid bacteria may be added to sterilized soymilk so as to be 1 × 10 ⁵ to 1 × 10 ⁷ cfu / mL, and left at 25 to 35 ° C. for 20 to 50 hours.

  The method of the present invention may comprise a fermentation process or may further include other processes. For example, the step of preparing soy milk may be performed before the fermentation step.

Fermented soymilk:
Fermented soymilk can be obtained by the above production method. The fermented soymilk of the present invention contains β-glucan derived from lactic acid bacteria and has a unique property and texture.

The fermented soymilk of the present invention has almost no whey separation, is viscous, and exhibits stringiness. The viscosity measured at 25 ° C. with a rotating cylindrical viscometer is, for example, 4000 to 10000 mPa · s, 5000 to 9000 mPa · s, or 6000 to 8000 mPa · s. An example of the rotating cylindrical viscometer is a viscometer VS-10 (Lion). Moreover, the measurement of a viscosity can be performed on the following conditions, for example.
Viscosity measurement conditions:
Rotor rotation speed: 60rpm
Rotor size: 2.7cm (diameter) x 4.5cm
Outer cylinder size: 4.8cm (diameter) x 6.5cm

Moreover, the fermented soymilk of the present invention has a relatively small viscosity change when the shear rate is changed, and exhibits a relatively large viscosity even at a high shear rate. Therefore, it is hard to collapse when put in the mouth, and the smoothness is maintained in the mouth until swallowed. When the viscosity at a shear rate of 10 s ⁻¹ and 50 s ⁻¹ measured at 25 ° C. is η ₁₀ and η ₅₀ , respectively, in the fermented soymilk of the present invention, η ₅₀ / η ₁₀ is 0.3 to 1 0.0, 0.4 to 0.8, or 0.4 to 0.6, and η ₅₀ is, for example, 500 to 1500 mPa · s or 800 to 1200 mPa · s.

  If a lactic acid bacterium having the ability to convert ornithine and / or citrulline is used during fermentation, a fermented soymilk containing ornithine and / or citrulline is obtained. That is, in one aspect, the fermented soymilk of the present invention contains ornithine and / or citrulline.

Food containing fermented soymilk:
The fermented soybean milk can be used as a food or food material. For example, it can be used as a dessert or a material thereof, and as a material for an emulsified seasoning (mayonnaise, dressing, etc.).

  Hereinafter, based on an Example, this invention is demonstrated more concretely. However, the present invention is not limited to the following examples.

Example 1 (Search for β-glucan-producing lactic acid bacteria):
From lactic acid bacteria strain 83 (Lactobacillus genus: 76 strains; Streptococcus genus: 5 strains; Pediococcus genus: 2 strains) possessed by Sapporo Breweries, β-glucan producing lactic acid bacteria as follows Explored.

Culture of lactic acid bacteria:
Each lactic acid bacterium was inoculated into MRS liquid medium (4 mL), and cultured at 30 ° C. in an anaerobic box for 2 to 3 days. The culture solution of Lactobacillus brevis SBC8027 was viscous and showed stringiness.

Extraction of genomic DNA:
0.5 mL of the culture solution was placed in an Eppendorf tube and centrifuged at 10,000 rpm for 5 minutes to recover the cells. The obtained cells were suspended in 0.1 mL of DNA extract (PrepMan Ultra), heated at 99 ° C. for 15 minutes, and further centrifuged at 10,000 rpm for 5 minutes. The supernatant was collected to obtain a genomic DNA solution.

PCR:
PCR was performed under the following conditions using two types of primer sets (below) corresponding to different regions (gtf primer region and mGTF primer region) on the β-glucan synthesis gene (gtf gene), using the genomic DNA of each lactic acid bacterium as a template. It was. Only for Lactobacillus brevis SBC8027, amplification of two DNA fragments was observed.
Gtf primer region:
Forward primer: 5′-AACGCCTGCAGACTGGCACC-3 ′ (SEQ ID NO: 1)
Reverse primer: 5′-ACCAGCCACCTCTCAACGCTTCG-3 ′ (SEQ ID NO: 2)
MGTF primer region:
Forward primer: 5′-CGGTAATGAAGCGTTTCCTG-3 ′ (SEQ ID NO: 3)
Reverse primer: 5′-GCTAGTACGGTAGACTTG-3 ′ (SEQ ID NO: 4)
・ PCR conditions:
Reaction solution: Lithos qPCR ^™ HotStart Master Mix-2 mM (Eurogentec)
Reaction temperature: denaturation (95 ° C., 10 minutes) → [denaturation (95 ° C., 15 seconds) → annealing (55 ° C., 5 seconds) → extension (72 ° C., 20 seconds)] × 40 cycles

Genetic analysis:
For Lactobacillus brevis SBC8027, the nucleotide sequences of two types of DNA fragments were determined and compared with the gtf gene sequence of Lactobacillus suebicus CUPV221 (accession number: GU174474 (GU1744474.1)). For gene analysis, MicroSeq ver. 1.4.3 (Applied Biosystems) was used.

  The alignment results were as shown in FIGS. FIG. 1 shows an alignment result of the gtf primer region of Lactobacillus suevicus CUPV221 (base sequence at positions 314 to 773 in the gtf gene (SEQ ID NO: 6)) and the corresponding DNA fragment derived from Lactobacillus brevis SBC8027 (SEQ ID NO: 5). It is. FIG. 2 shows the alignment result of the mGTF primer region (base sequence at positions 981 to 1390 in the gtf gene (SEQ ID NO: 8)) of Lactobacillus suevicus CUPV221 and the corresponding DNA fragment derived from Lactobacillus brevis SBC8027 (SEQ ID NO: 7). It is. 1 and 2, “SEQ-5”, “SEQ-6”, “SEQ-7”, and “SEQ-8” represent the nucleotide sequences of SEQ ID NOs: 5 to 8, respectively. Moreover, “-” in the sequence represents a gap. As is clear from FIGS. 1 and 2, homology of about 99% was observed in the gtf primer region and about 96% in the mGTF primer region.

Antibody treatment:
For Lactobacillus brevis SBC8027, cells grown on MRS agar plates were treated with Streptococcus pneumoniae type 37-specific antiserum (Statens Serum Institute) on a slide glass. Streptococcus pneumoniae type 37 specific antiserum is known to react with lactic acid bacteria β-glucan. When the presence or absence of aggregation was observed with an optical microscope, aggregation was observed (FIG. 3). In FIG. 3, (a) is a photomicrograph before antibody treatment, and (b) is a photomicrograph after antibody treatment. In (b), aggregation is observed within the round frame.

  From the above, it was found that Lactobacillus brevis SBC8027 has β-glucan production ability.

Purification of exopolysaccharide:
A glycerol frozen stock solution of Lactobacillus brevis SBC8027 was inoculated into MRS liquid medium (4 L) and cultured at 30 ° C. for 40 to 60 hours. After culturing, the cells were heat-treated in a hot water bath (60 ° C.) for 20 minutes, cooled, and centrifuged at 8000 rpm for 30 minutes to remove cells and heat-denatured products. 1N Hydrochloric acid was added to the resulting solution to lower the pH to 3.0, and then the solution was passed through Hokuetsu HS resin (Ajinomoto Fine Techno) (column size: 2.6 × 50 cm) equilibrated with pure water. The non-adsorbed fraction was collected. This was concentrated under reduced pressure to about 1 L, 2 times the amount of acetone was added, and the supernatant was removed by decantation to obtain a light brown precipitate. After dissolving the precipitate in pure water, this was passed through Diaion PA308 (Mitsubishi Chemical) (column size: 2.6 × 50 cm) equilibrated with pure water, and the non-adsorbed fraction was collected. Next, this was passed through Diaion PK208 (Mitsubishi Chemical) (column size: 2.6 × 50 cm) equilibrated with pure water, and the non-adsorbed fraction was collected. This was again passed through Diaion PA308 (Mitsubishi Chemical) (column size: 2.6 × 50 cm) equilibrated with pure water, and the non-adsorbed fraction was collected. After concentrating this to about 500 mL under reduced pressure, twice as much acetone was added, and the supernatant was removed by decantation to obtain a milky white precipitate. After dissolving the precipitate in pure water, acetone was added thereto to form a precipitate. The obtained precipitate was dissolved again in pure water, then acetone was removed by concentration under reduced pressure, and freeze-dried to obtain a white cotton-like preparation (extracellular polysaccharide).

Gel filtration chromatography:
After dissolving 30 mg of the sample (extracellular polysaccharide) in 5 mL of pure water, this was passed through a TOYOPEARL HW-65 column (Tosoh) equilibrated with a 0.25 M NaCl aqueous solution (column size: 2.6 × 60 cm). The elution fractions were collected in 12 mL portions. About each fraction, the total amount of sugars (glucose conversion) was measured by the phenol-sulfuric acid method, and the total amount of sugars obtained was multiplied by 0.9 to obtain the amount of polysaccharides. The results (chromatogram) were as shown in FIG. In FIG. 4, the numbers on the horizontal axis represent fraction numbers (1 to 20 in the order of elution).

Monosaccharide composition analysis:
Fractions 5-7 were combined as fraction A, fractions 8-9 as fraction B, fractions 10-11 as fraction C, and fractions 12-14 as fraction D, then desalted by dialysis, It was dried under reduced pressure. This was dissolved in 2 mL of 4M trifluoroacetic acid (TFA), transferred to a test tube with a screw cap, and then allowed to stand in an oil bath (100 ° C.) for 3 hours. The reaction solution was dried under reduced pressure, pure water was added, and then again dried under reduced pressure to sufficiently remove TFA. It was dissolved in pure water so that the final liquid volume was 5 mL, and this was used as a sample liquid for analysis. The sample solution was subjected to a reducing sugar analytical HPLC system (post column method) (Shimadzu). The result was as shown in FIG. The polysaccharide of fraction A was estimated to be β-glucan because most of the constituent monosaccharides were glucose and the molecular weight (in terms of pullulan) was several hundred thousand Da.

¹ H-NMR:
A ¹ H-NMR spectrum of fraction A was measured. The measurement of ¹ H-NMR spectrum was performed by JNM-ECA500 (JEOL) using D ₂ O as a solvent. The result (spectrum) was as shown in FIG. Further, signals observed at 4.5 to 5.0 ppm were as follows. These results confirmed that the polysaccharide in fraction A was β-glucan.
δ (ppm): 4.53 (d, J = 5.5 Hz), 4.95 (d, J = 6.5 Hz).

Example 2 (production of fermented soymilk):
45 mL of soy milk having the following composition was placed in an 80 mL jam bottle and sterilized by heating at 80 ° C. for 1 hour. After cooling to room temperature, a glycerol cryopreservation solution of Lactobacillus brevis SBC8027 was added to 2.6 × 10 ⁶ cfu / mL, and left to stand at 30 ° C. for 17 or 24 hours to obtain a soymilk fermented product. .
Composition of soy milk:
Unadjusted soymilk (Marsan Eye): 83mL
Malt extract (MALTAX10): 5g
Gum syrup (Kamijima Coffee): 5g
Sucrose: 7g
Yogurt flavor (Takasago Incense): 0.2mL

About the obtained soymilk fermented product, viable cell count, pH, and acidity were measured. The number of viable bacteria was measured as follows. 1 g of soy milk fermented product is put in a sterile container, 9 mL of sterile physiological saline is added and suspended, and then 1 × 10 ⁴ times (17 hours fermented product) or 1 × 10 ⁵ times (24 hours of fermented product) with sterile physiological saline. Diluted to fermented product). Then, 0.1 mL of the diluted solution was applied to the MRS agar plate, cultured for 2 days under anaerobac anaerobic conditions at 30 ° C., and the number of colonies that appeared was counted.

The results are shown in Table 1.

Example 3 (production of fermented soymilk):
45 mL of soy milk having the following composition was placed in an 80 mL jam bottle and sterilized by heating at 80 ° C. for 1 hour. After cooling to room temperature, a glycerol cryopreservation solution of Lactobacillus brevis SBC8027 was added to 2.6 × 10 ⁶ cfu / mL, and the mixture was allowed to stand at 30 ° C. for 24 hours to obtain a fermented soymilk.
Composition of soy milk:
Unadjusted soymilk (Marsan Eye): 83mL
Malt extract (MALTAX10): 5g
Gum syrup (Kamijima Coffee): 5g
Sucrose: 7g
Yogurt flavor (Takasago Incense): 0.2mL

When the viscosity of the obtained soymilk fermented product and commercially available yogurt (Caspian Sea yogurt (Fujikko)) was measured at 25 ° C. using a rotating cylindrical viscometer (Viscometer VS-10 (Lion)) under the following conditions: It was 8000 mPa · s for fermented soymilk and 5000 mPa · s for Caspian Sea yogurt. Caspian Sea yogurt is a yogurt obtained by fermenting milk with lactic acid bacteria such as Lactococcus cremoris.
Viscosity measurement conditions:
Rotor rotation speed: 60rpm
Rotor size: 2.7cm (diameter) x 4.5cm
Outer cylinder size: 4.8cm (diameter) x 6.5cm

Moreover, about the fermented soybean milk and the Caspian Sea yogurt, the viscosity was measured using a dynamic viscoelasticity measuring apparatus (VAR-50) while changing the shear rate under the following conditions. All samples were stirred in a beaker before measurement.
Viscosity measurement conditions:
Sample amount: 2g
Measurement mode: Constant speed mode Measurement temperature: 25 ℃ ± 0.2 ℃
MS geometry: cone type 40mm-4 °
Shear rate (s ⁻¹ ): 1.00E + 01 to 8.00E + 01
Shear rate increase type: Linear increase Delay time: 10 seconds Integration time: 10 seconds Number of measurement points: 15 points Regulator strength: 40%

The results were as shown in FIG. In FIG. 7 and Table 2, “SBC8027” represents a soymilk fermented product of Lactobacillus brevis SBC8027. Further, in the table, eta _10, eta ₅₀ each shear rate ^10s -1, represents the viscosity at ^{50s -1.}

  As is clear from the above results, the viscosity of Lactobacillus brevis SBC8027 soymilk fermented product was higher, but there was no significant difference between the two in terms of viscosity change when the shear rate was changed. In fact, in the soymilk fermented product of Lactobacillus brevis SBC8027, no separation of whey was observed, and it was more viscous and stronger in stringiness than Caspian Sea yogurt. In addition, like Caspian Sea yogurt, it was hard to collapse when put in the mouth, and maintained smoothness in the mouth until swallowed.

Example 4 (Production of fermented soybean milk):
Fermented soymilk was produced in the same manner as in Example 3 using Lactobacillus brevis SBC8027. Further, a soymilk fermented product was produced in the same manner as in Example 3 using a β-glucan non-producing lactic acid bacterium belonging to Lactobacillus brevis (hereinafter referred to as “lactic acid bacterium X” in this example) instead of Lactobacillus brevis SBC8027. .

The viscosity of the two soymilk fermented products obtained was measured under the following conditions using a rotating cylindrical viscometer (Viscometer VS-10 (Lion)) at a temperature of 25 ° C., and the soymilk fermented product of Lactobacillus brevis SBC8027 Was 6500 mPa · s, and the soymilk fermented product of lactic acid bacteria X was 2800 mPa · s.
Viscosity measurement conditions:
Rotor rotation speed: 60rpm
Rotor size: 2.7cm (diameter) x 4.5cm
Outer cylinder size: 4.8cm (diameter) x 6.5cm

  Moreover, about 2 types of fermented soybean milk, the viscosity was measured changing the shear rate similarly to Example 3. FIG. Furthermore, the viscosity of the commercially available soymilk fermented product (soymilk glut (Marsan Eye)) was also measured while changing the shear rate. Soymilk Glut is a fermented soymilk produced using lactic acid bacteria belonging to Lactobacillus delbrueckii (having an extracellular polysaccharide-producing ability, but not β-glucan-producing ability).

The results were as shown in FIGS. 8 and 9 and Table 3, “SBC8027” and “lactic acid bacteria X” represent Lactobacillus brevis SBC8027 and lactic acid bacteria X soymilk fermentation products, respectively. Further, in the table, eta _10, eta ₅₀ each shear rate ^10s -1, represents the viscosity at ^{50s -1.} The data in FIGS. 8 and 9 for the soymilk fermented Lactobacillus brevis SBC8027 are identical.

As is clear from the above results, the soymilk fermented product of Lactobacillus brevis SBC8027 was higher in viscosity than the soymilk fermented product of lactic acid bacteria X or a commercially available soymilk fermented product. In addition, the change in viscosity when the shear rate was changed was smaller in the fermented soymilk of Lactobacillus brevis SBC8027, particularly at a shear rate of 10 to 20 s ⁻¹ . In fact, the soymilk fermented product of Lactobacillus brevis SBC8027 was more viscous and stronger in stringiness than the soymilk fermented product of lactic acid bacteria X or a commercially available soymilk fermented product. In addition, fermented soymilk of lactic acid bacteria X and commercially available soymilk fermented products were easy to collapse when put in the mouth, but fermented soymilk of Lactobacillus brevis SBC8027 is difficult to collapse when put in the mouth and is swallowed until swallowed. The smoothness was maintained.

Example 5 (production of fermented soymilk):
Fermented soymilk was produced in the same manner as in Example 3 using Lactobacillus brevis SBC8027. Three kinds of free amino acids (arginine (Arg), ornithine (Orn), and citrulline (Cit)) were measured for the soymilk before fermentation and the fermented soymilk after fermentation.

The results are shown in Table 4.

  As is apparent from Table 4, arginine in soy milk disappeared by fermentation using Lactobacillus brevis SBC8027, and ornithine and citrulline were produced instead (conversion rate: 90%).

Example 6 (Production of fermented soybean milk):
After adding 0.1% of arginine hydrochloride to the soy milk, a fermented soy milk was produced in the same manner as in Example 5 using this soy milk. Three kinds of free amino acids (arginine (Arg), ornithine (Orn), and citrulline (Cit)) were measured for the soymilk before fermentation and the fermented soymilk after fermentation.

The results are shown in Table 5.

  As is apparent from Table 5, arginine in soy milk disappeared by about 3 minutes and 2 by fermentation using Lactobacillus brevis SBC8027, and ornithine and citrulline were generated instead (conversion rate: 55%).

  According to Examples 5 and 6, Lactobacillus brevis SBC8027 has the ability to convert arginine to ornithine and citrulline, and fermenting soy milk using such lactic acid bacteria, soy milk fermented product containing ornithine and citrulline It was confirmed that Moreover, when using such lactic acid bacteria, it was confirmed that fermented soymilk containing more ornithine and citrulline is obtained by adding arginine to soymilk and performing fermentation.

Claims (3)

A method for producing a fermented soymilk comprising a step of fermenting soymilk using lactic acid bacteria having β-glucan producing ability ,
The method wherein the lactic acid bacterium is Lactobacillus brevis SBC8027 (Accession Number: FERM BP-10630) . The method according to claim 1, wherein the soymilk fermented product is a fermented soymilk having a viscosity of 4000 to 10,000 mPa · s measured at 25 ° C. with a rotating cylindrical viscometer. The fermented soymilk is η ₅₀ / Η ₁₀ [Η ₁₀ , Η ₅₀ Each has a shear rate of 10 s measured at 25 ° C. ^-1 50s ^-1 It represents the viscosity at. ] Is the fermented soybean milk of 0.3 or more.