JP5539254B2 - Biological material analysis apparatus and biological material analysis method - Google Patents

Description

  The present invention relates to a biological material analysis apparatus and a biological material analysis method, and in particular, a development layer for developing a spotted specimen sample, and a reagent that generates a substance that develops color by reacting with a test object in the specimen sample. The present invention relates to a biological material analyzer and a biological material analysis method using an analysis chip including a reaction layer.

  When analyzing a specimen sample of biological material (whole blood, plasma, serum, etc.) using the analysis chip as described above, the specimen sample is deposited on the development layer of the analysis chip. When the development layer is developed and reaches the reaction layer, the test object in the specimen sample reacts with the reagent in the reaction layer to generate a color substance. When the reaction layer is irradiated with detection light including light having a wavelength that is absorbed by the coloring substance from the light source, the detection light is absorbed by the dye, and thus diffuse reflected light from the reaction layer is reduced. A correlation between the amount of decrease and the amount of substance of the inspection object is obtained in advance, and the amount of substance of the inspection object can be quantified from this correlation equation (calibration curve).

  However, the analysis chip due to the coating layer of the emulsion used for the spreading layer may become non-uniform, or the specimen sample may spread non-uniformly in the spreading layer due to poor spot application of the specimen sample. In such cases, the method of irradiating detection light on the entire surface of the reaction layer to be inspected and detecting diffuse reflection light from the entire surface of the reaction layer on the surface of the reaction layer cannot perform high-precision measurement. There's a problem.

  In Patent Document 1, reflected light from a plurality of different areas of a physiological test specimen is detected by a CCD that is a two-dimensional detector, and the reflectance value from an area that has a sufficient sample is detected. A technique is disclosed that derives the analytical concentration and does not use reflectance values from each region determined to have no sufficient sample.

JP 2004-163393 A

  The biological material contains water, and the biological material can be detected in the atmosphere by using light having a wavelength that is absorbed in the water and not absorbed in the atmosphere, preferably near infrared light. However, the CCD is sensitive only to light having a wavelength shorter than 0.8 μm, and other two-dimensional sensors are the same. There are special two-dimensional sensors sensitive to near infrared light, but they are very expensive. A system using the present dry analysis chip having a development layer and a reaction layer is often used in a POCT (small diagnostic machine) that has a high need for low cost and small size, and thus an expensive special light receiving element cannot be adopted.

  The main object of the present invention is to provide inexpensive near-infrared light even when the analysis chip for analyzing biological material is non-uniform or when the specimen sample containing the biological substance is not uniformly developed in the analysis chip. It is an object of the present invention to provide a biological material analyzing apparatus and a biological material analyzing method capable of analyzing a specimen sample containing a biological material using the above detector.

According to the present invention,
An analysis chip holding means for holding an analysis chip for analyzing a biological material;
A first light source for irradiating each of the plurality of regions of the analysis chip with a plurality of first beams composed of light including a near infrared region component;
A second light source for respectively irradiating the plurality of regions of the analysis chip with a plurality of second beams made of detection light for detecting a detection substance generated on the analysis chip by reaction with the biological material;
A first detector for detecting the near infrared region component;
A second detector for detecting the detection light;
The first output light from the plurality of regions of the analysis chip when the plurality of first beams are irradiated to the plurality of regions of the analysis chip, respectively, are incident on the first detector, and An optical system that causes the second output light from the plurality of regions of the analysis chip to be incident on the second detector when the plurality of second beams are irradiated to the plurality of regions of the analysis chip, respectively .
The plurality of first beams are irradiated to the plurality of regions of the analysis chip, respectively, and the plurality of first output lights from the plurality of regions of the analysis chip are detected by the first detector. The normal area of the plurality of areas is specified by the acquired plurality of data, and only the plurality of areas specified as the normal area are irradiated with the plurality of second beams, respectively. Quantification of the test object of the biological material based on a plurality of data obtained by detecting the second output light from only the plurality of regions identified as the normal region of the analysis chip by the second detector. And a biological material analyzer comprising: control means for performing control .

Also preferably,
The first light source includes a plurality of first sub-light sources that respectively emit the plurality of first beams irradiated to the plurality of regions of the analysis chip,
The second light source includes a plurality of second sub-light sources that respectively emit the plurality of second beams irradiated to the plurality of regions of the analysis chip,
The control means controls the first light source, the second light source, the first detector, and the second detector to sequentially turn on the plurality of first sub-light sources, and A plurality of first beams are sequentially irradiated onto the plurality of regions of the analysis chip, and each first output light from the plurality of regions of the analysis chip is sequentially detected by the first detector. The second sub-light source is sequentially turned on to sequentially irradiate the plurality of regions with the plurality of second beams, and the second output light from the plurality of regions of the analysis chip The detection is sequentially performed by the second detector.

  Preferably, the normal region is specified by irradiating each of the plurality of regions of the analysis chip with the plurality of first beams, and each of the first outputs from the plurality of regions of the analysis chip. The detection is performed by setting a region in which data is detected within the average ± standard deviation of a plurality of data detected by the first detector as a normal region.

  Preferably, the first detector is a zero-dimensional near infrared sensor.

  Preferably, the analysis chip includes a development layer for developing a specimen sample containing the biological material, and a reaction layer having a reagent that generates a substance that develops color by reacting with the test object in the biological material. I have.

Moreover, according to the present invention,
Irradiating the plurality of regions of the analysis chip for analyzing the biological material from the first light source with the plurality of first beams made of light including near-infrared region components, respectively. The step of causing the first output light respectively emitted from the plurality of regions of the analysis chip to enter the first detector for detecting the near infrared region component and detect the first output light,
The plurality of regions of the analysis chip are respectively irradiated with a plurality of second beams made of detection light for detecting a detection substance generated on the analysis chip by a reaction with the biological material from a second light source, Irradiating the second output light emitted from each of the plurality of regions of the analysis chip by irradiating the second beam with a second detector for detecting the detection light, and detecting the second output light. Prepared ,
The plurality of first beams are irradiated to the plurality of regions of the analysis chip, respectively, and the plurality of first output lights from the plurality of regions of the analysis chip are detected by the first detector. The normal area of the plurality of areas is specified by the acquired plurality of data, and only the plurality of areas specified as the normal area are irradiated with the plurality of second beams, respectively. Quantification of the test object of the biological material based on a plurality of data obtained by detecting the second output light from only the plurality of regions identified as the normal region of the analysis chip by the second detector. A biological material analysis method is provided.

Preferably,
The first light source includes a plurality of first sub-light sources that respectively emit the plurality of first beams irradiated to the plurality of regions of the analysis chip,
The second light source includes a plurality of second sub-light sources that respectively emit the plurality of second beams irradiated to the plurality of regions of the analysis chip,
The plurality of first sub-light sources are sequentially turned on to sequentially irradiate the plurality of first beams onto the plurality of regions of the analysis chip, and the respective first outputs from the plurality of regions of the analysis chip. Light is sequentially detected by the first detector, the plurality of second sub-light sources are sequentially turned on, the plurality of second beams are sequentially irradiated onto the plurality of regions of the analysis chip, and the analysis is performed. Each second output light from the plurality of regions of the chip is sequentially detected by the second detector.

  Preferably, the normal region is specified by irradiating each of the plurality of regions of the analysis chip with the plurality of first beams, and each of the first outputs from the plurality of regions of the analysis chip. The detection is performed by setting a region in which data is detected within the average ± standard deviation of a plurality of data detected by the first detector as a normal region.

  Preferably, the first detector is a zero-dimensional near infrared sensor.

  Preferably, the analysis chip includes a development layer for developing a specimen sample containing the biological material, and a reaction layer having a reagent that generates a substance that develops color by reacting with the test object in the biological material. I have.

  According to the present invention, even when the analysis chip for analyzing the biological material is non-uniform or when the specimen sample containing the biological substance is not uniformly developed in the analysis chip, inexpensive near-infrared light detection is possible. There are provided a biological material analyzer and a biological material analysis method capable of analyzing a specimen sample containing a biological material using a vessel.

It is a schematic block diagram for demonstrating the biological material analyzer and biological material analysis method of the 1st Embodiment of this invention. FIG. 3 is a schematic diagram for explaining non-uniformity of the analysis chip 10 due to an emulsion coating 26 used for the spreading layer 18. 4 is a schematic diagram for explaining non-uniformity due to defective spotting of a specimen 20 or the like. FIG. It is a figure which shows absorption of water. It is a figure which shows atmospheric absorption. It is a figure which shows the relationship between the reflected light of near-infrared light from the analysis chip used for the biological material analysis apparatus and biological material analysis method of embodiment of this invention, and time. It is the schematic for demonstrating the biological material analysis method of embodiment of this invention. It is a flowchart for demonstrating an example of the biological material analysis method of embodiment of this invention. It is a flowchart for demonstrating the other example of the biological material analysis method of embodiment of this invention.

  Hereinafter, preferred embodiments of the present invention will be described with reference to the drawings.

  First, the biological material analyzer of the first embodiment of the present invention will be described with reference to FIG. The biological material analysis apparatus 100 according to the first embodiment of the present invention includes an analysis chip holding unit 30 that holds an analysis chip 10 for analyzing a biological material, a reading head 60, and a computer 70. The reading head 60 includes a detection light source 40, a near-infrared light source 50, a detector 62, an optical system 61 that causes light from the analysis chip 10 to enter the detector 62, and a die lock mirror 65. . The die lock mirror 65 transmits the detection light beam 45 from the detection light source 40 and reflects the near infrared light beam 55 from the near infrared light source 50. The computer 70 controls the detection light source 40, the near-infrared light source 50, and the detector 62, and performs calculations described later.

  The analysis chip 10 includes a reaction layer 14 provided on a transparent support 12 made of PET or the like, a reflection layer 16 provided on the reaction layer 14, and a development layer 18 provided on the reflection layer 16. ing. In the development layer 18, when a specimen sample 20 containing a biological material is spotted, the development layer 18 develops by capillary action. The reflective layer 16 is used to block the optical influence of the specimen sample 20 containing a biological material, but may be omitted depending on the type of the specimen sample 20 and the wavelength of light from the light source. The reaction layer 14 includes a reagent that generates a substance that develops color by reacting with the test object in the sample 20.

  In the reaction layer 14, light (detection light) including light of a wavelength that is absorbed by a substance that develops color when this reagent reacts with the test object in the sample 20 is sent to the detection region 22 of the analysis chip 10. Is absorbed, transmitted and reflected by the analysis chip 10 (mainly by the reaction layer 14). By detecting diffusely reflected light among the reflected light from the analysis chip 10, the test object in the sample 20 can be quantified. The detection light is irradiated to the inspection target region 22 of the analysis chip 10, but as shown in FIG. 2, the analysis chip 10 becomes non-uniform due to the emulsion coating 26 used for the spreading layer 18, or the specimen sample 20. As shown in FIG. 3, the specimen sample 20 may ununiformly develop in the development layer 18 to form a nonuniform development region 24 in the inspection target region 22 due to the spotting failure or the like. In such a case, the method of irradiating the entire surface of the inspection target region 22 of the analysis chip 10 with detection light and detecting the diffuse reflected light from the entire surface of the inspection target region 22 of the analysis chip 10 is highly accurate. Cannot measure

  Therefore, in the present embodiment, as shown in FIG. 1, the inspection target region 22 of the analysis chip 10 is virtually divided into a plurality of regions, and the detection light is respectively assigned to the plurality of regions in one-to-one correspondence. A detection light source 40 including a plurality of LEDs 41 that respectively irradiate is used. When the detection light beams 45 are irradiated from the plurality of LEDs 41 to the plurality of regions of the inspection target region 22, a plurality of beam spots 43 are generated in the inspection target region 22. Diffuse reflected light of detection light output from the analysis chip 10 is made incident on the detectors 62 by the optical system 61 with respect to the plurality of beam spots 43, respectively. The detector 62 includes a silicon photodiode (not shown), and detects the detection light output from each analysis chip 10. In the present embodiment, the plurality of LEDs 41 are sequentially turned on, and the plurality of detection light beams 45 are sequentially irradiated onto the plurality of regions of the inspection target region 22, and at that time, are sequentially output from the inspection target region 22 of the analysis chip 10. The detection light is sequentially detected by the silicon photodiode of the detector 62.

  Next, water absorption and atmospheric absorption will be described with reference to FIGS. FIG. 4 is a diagram illustrating water absorption, and FIG. 5 is a diagram illustrating atmospheric absorption. It can be seen that there is a wavelength that penetrates the atmosphere and is strongly absorbed by water in the near-infrared wavelength range (0.7 to 2.5 μm). 1.4 [mu] m or less, 1.6 to 1.9 [mu] m, and 2.0 to 2.5 [mu] m aiming at the atmospheric window roughly correspond to the wavelength. Since the biological material contains water, the biological material can be detected in the atmosphere by using light including light of this wavelength.

  Therefore, in the present embodiment, as shown in FIG. 1, a near-infrared light source 50 that emits light in the near-infrared wavelength range (0.7 to 2.5 μm) through the inspection target region 22 of the analysis chip 10. Is used. The near-infrared light source 50 has a one-to-one correspondence with light in the near-infrared (0.7 to 2.5 μm) wavelength region into a plurality of regions obtained by virtually dividing the inspection target region 22 of the analysis chip 10. Are provided with a plurality of infrared LEDs 51 that respectively irradiate. Note that the wavelength of the near infrared light emitted from the infrared LED 51 is preferably 1 μm or more, and more preferably 1.5 μm or more.

  When the near-infrared light beams 55 are irradiated from the plurality of LEDs 51 to the plurality of regions of the inspection target region 22, a plurality of beam spots 53 are generated in the inspection target region 22. Near-infrared reflected light (diffuse reflected light and regular reflected light) respectively output from the analysis chip 10 is incident on the detectors 62 by the optical system 61 with respect to the plurality of beam spots 53. The detector 62 includes a thermopile (not shown) that is a zero-dimensional sensor sensitive to near infrared rays, and detects near infrared light output from the analysis chip 10. In this figure, the lens 61 is drawn so as to collect the light from the LED 41. However, it is difficult to collect the infrared light with the lens for the LED 41, and the light from the LED 51 is not sufficiently converged. The scattered light of the LED 51 that is larger than the diode is incident on 62. If a thermopile is placed adjacent to the photodiode, the scattered light from the LED 51 can be received. In the present embodiment, a plurality of LEDs 51 are sequentially turned on, and a plurality of near-infrared light beams 55 are sequentially irradiated onto a plurality of regions of the inspection target region 22, and at that time, sequentially output from the inspection target region 22 of the analysis chip 10. The detected near infrared light is sequentially performed by the thermopile of the detector 62. The plurality of beam spots 53 irradiate the plurality of regions of the inspection target region 22 with the near infrared light beam 55 and the detection light beam 45 so as to overlap the plurality of beam spots 43, respectively.

  In the dry biochemical test using the analysis chip 10 as in the present embodiment, the specimen sample 20 spreads in the development layer (membrane) 18 after the spotting by capillary action and reacts through the reflective layer 16. It reaches the layer 14 where it reacts with the reagents in the reaction layer 14 to produce a colored material. Then, when the intensity of the diffuse reflected light from the analysis chip 10 (mainly from the reaction layer 14) with respect to the light from the detection light source 40 is detected by a photodiode (not shown) of the detector 62, an inspection in the specimen sample 20 is performed. The concentration of the object can be measured. A calibration curve indicating the relationship between the intensity of the diffusely reflected light detected by the photodiode and the concentration of the test object in the specimen sample 20 is obtained in advance and stored in the computer 60. The concentration of the test object in the specimen sample 20 is obtained by the computer 70 from the intensity of the diffuse reflected light detected by the diode.

  In the dry-type biochemical test using the analysis chip 10 as in the present embodiment, after the specimen sample 20 is spotted on the development layer 18, the inside of the development layer (membrane) 18 spreads by capillary action. When the analysis chip (slide) 10 is viewed from the vertical direction, the specimen 20 is developed two-dimensionally. Depending on how the specimen 20 is spotted, as shown in FIG. A non-uniform development region 24 is formed in the region 22.

  FIG. 6 is a diagram showing the relationship between the reflected light of near infrared light from the analysis chip 10 and time. The horizontal axis represents time, and the vertical axis represents the amount of reflected near-infrared light detected by the thermopile in the detector 62. When the specimen sample 20 containing a biological material is spotted, the amount of reflected light first suddenly decreases (starts spotting), and then the specimen sample 20 develops in the spreading layer 18 by capillary action. I will do it. When the development of the specimen sample 20 in the development layer 18 is completed, the amount of reflected light is saturated and constant, so that the completion of the development of the specimen sample 20 can be detected. Since the amount of reflected light decreases with time in this way, the extent of the decrease is detected. For example, if the amount of reflected light at a certain point in time is detected, a slow-development region is detected and the inspection target region 22 is detected. It is possible to detect the non-uniform development region 24.

  The emulsion smear 26 used for the spreading layer 18 also detects the reflected light from the analysis chip 10 of the near-infrared light from the plurality of LEDs 51 with the thermopile in the detector 62. It is possible to detect the smear 26 of the emulsion.

  An area excluding the non-uniform development area 24 and the emulsion smear 26 in the plurality of areas to be inspected 22 is specified as a normal area, and the inspection specified as the normal area for the light from the detection light source 40 is specified. The test object 20 in the sample 20 is quantified using only data obtained by detecting the light output from the target region 22 with the photodiode in the detector 62.

  In order to specify the normal region using near infrared light from the plurality of LEDs 51, for example, the average value of the amount of reflected light of the near infrared light from the analysis chip 10 ± the light amount within the standard deviation is set. The detected area is set as a normal area.

  Further, in order to use only the data output from the region of the inspection target region 22 identified as the normal region by the photodiode in the detector 62, as shown in FIG. The near-infrared light beam 55 is used to irradiate a plurality of regions of the inspection target region 22 with the near-infrared light beam 55, and a plurality of output lights from the plurality of regions of the inspection target region 22 are detected (step 101). ), A normal region is identified based on the light amounts of the plurality of output lights (step 102). Thereafter, only the region identified as the normal region is irradiated with the plurality of detection light beams 45 from the plurality of LEDs 41, and Detection light output only from the specified region may be detected by the photodiode of the detector 62 (step 103), and only the detected data may be used (step 104). 9, the near infrared light from the plurality of LEDs 51 is used in advance to irradiate the plurality of near infrared light beams 55 to the plurality of regions of the inspection target region 22, respectively. A plurality of output lights from the region are detected (step 201), and a plurality of detection light beams 45 are emitted from the plurality of LEDs 41 to the plurality of regions of the inspection target region 22, respectively. Are detected (step 202), and the normal region is specified by the data of the plurality of output lights from the plurality of regions of the inspection target region 22 obtained by using the near infrared light from the plurality of LEDs 51. (Step 203) Of the output light data from the plurality of regions of the inspection target region 22 obtained using the detection light from the plurality of LEDs 41, data from only the region identified as the normal region is used. Also good (step 204).

  Referring to FIG. 7, for example, in the case of a rate method in which measurement is performed for a plurality of times (two points in FIG. 7), the specimen sample 20 is spotted on the analysis chip 10 (sample spotting), and then the measurement is performed. The measurement is performed twice (measurement 1, measurement 2) (see FIG. 7B). In the present embodiment, measurement is performed using near-infrared light from a plurality of LEDs 51 before the sample is spotted (measurement A). Then, measurement is also performed between measurement 1 and measurement 2 (measurement B). In measurement A, the smear 26 of the emulsion used for the development layer 18 can be detected, and in measurement B, the non-uniform development area 24 in the inspection object area 22 can be detected.

  While various typical embodiments of the present invention have been described above, the present invention is not limited to these embodiments. For example, instead of placing two types of light receiving elements in 62, it is also possible to divide the optical path using a dichroic mirror and receive light respectively. Further, the LED 51 may receive regular reflected light instead of scattered reflected light. The scope of the present invention is limited only by the following claims.

DESCRIPTION OF SYMBOLS 10 Analysis chip 12 Transparent support 14 Reaction layer 16 Reflection layer 18 Deployment layer 20 Sample sample 22 Inspection object area | region 30 Analysis chip holding | maintenance part 40 Detection light source 41 LED for detection light
43 Beam Spot 50 Near Infrared Light Source 51 Near Infrared Light LED
53 Beam Spot 60 Reading Head 61 Optical System 62 Detector 65 Die-lock Mirror 70 Computer 100 Biological Material Analyzer

Claims (10)

An analysis chip holding means for holding an analysis chip for analyzing a biological material;
A first light source for irradiating each of the plurality of regions of the analysis chip with a plurality of first beams composed of light including a near infrared region component;
A second light source for respectively irradiating the plurality of regions of the analysis chip with a plurality of second beams made of detection light for detecting a detection substance generated on the analysis chip by reaction with the biological material;
A first detector for detecting the near infrared region component;
A second detector for detecting the detection light;
The first output light from the plurality of regions of the analysis chip when the plurality of first beams are irradiated to the plurality of regions of the analysis chip, respectively, are incident on the first detector, and An optical system that causes the second output light from the plurality of regions of the analysis chip to be incident on the second detector when the plurality of second beams are irradiated to the plurality of regions of the analysis chip, respectively .
The plurality of first beams are irradiated to the plurality of regions of the analysis chip, respectively, and the plurality of first output lights from the plurality of regions of the analysis chip are detected by the first detector. The normal area of the plurality of areas is specified by the acquired plurality of data, and only the plurality of areas specified as the normal area are irradiated with the plurality of second beams, respectively. Quantification of the test object of the biological material based on a plurality of data obtained by detecting the second output light from only the plurality of regions identified as the normal region of the analysis chip by the second detector. A biological material analyzer comprising: control means for performing control . The first light source includes a plurality of first sub-light sources that respectively emit the plurality of first beams irradiated to the plurality of regions of the analysis chip,
The second light source includes a plurality of second sub-light sources that respectively emit the plurality of second beams irradiated to the plurality of regions of the analysis chip,
The control means controls the first light source, the second light source, the first detector, and the second detector to sequentially turn on the plurality of first sub-light sources, and A plurality of first beams are sequentially irradiated onto the plurality of regions of the analysis chip, and each first output light from the plurality of regions of the analysis chip is sequentially detected by the first detector. The second sub-light source is sequentially turned on to sequentially irradiate the plurality of regions with the plurality of second beams, and the second output light from the plurality of regions of the analysis chip The biological material analyzer according to claim 1, wherein detection is sequentially performed by the second detector. The normal region is identified by irradiating the plurality of first beams to the plurality of regions of the analysis chip, respectively, and applying the first output light from the plurality of regions of the analysis chip to the first. The biological material analyzer according to claim 1 or 2 , wherein the normal region is a region in which data within an average ± standard deviation of a plurality of data detected by the detector is detected. It said first detector, living matter analysis apparatus according to any one of claims 1 to 3, which is a near infrared sensor zero-dimensional. The analysis chip includes a development layer for developing a specimen sample containing the biological material, and a reaction layer having a reagent that reacts with a test object in the biological material to generate a colored material. The biological material analyzer of any one of 1-4 . Irradiating the plurality of regions of the analysis chip for analyzing the biological material from the first light source with the plurality of first beams made of light including near-infrared region components, respectively. The step of causing the first output light respectively emitted from the plurality of regions of the analysis chip to enter the first detector for detecting the near infrared region component and detect the first output light,
The plurality of regions of the analysis chip are respectively irradiated with a plurality of second beams made of detection light for detecting a detection substance generated on the analysis chip by a reaction with the biological material from a second light source, Irradiating the second output light emitted from each of the plurality of regions of the analysis chip by irradiating the second beam with a second detector for detecting the detection light, and detecting the second output light. Prepared ,
The plurality of first beams are irradiated to the plurality of regions of the analysis chip, respectively, and the plurality of first output lights from the plurality of regions of the analysis chip are detected by the first detector. The normal area of the plurality of areas is specified by the acquired plurality of data, and only the plurality of areas specified as the normal area are irradiated with the plurality of second beams, respectively. Quantification of the test object of the biological material based on a plurality of data obtained by detecting the second output light from only the plurality of regions identified as the normal region of the analysis chip by the second detector. biological material analysis method for performing. The first light source includes a plurality of first sub-light sources that respectively emit the plurality of first beams irradiated to the plurality of regions of the analysis chip,
The second light source includes a plurality of second sub-light sources that respectively emit the plurality of second beams irradiated to the plurality of regions of the analysis chip,
The plurality of first sub-light sources are sequentially turned on to sequentially irradiate the plurality of first beams onto the plurality of regions of the analysis chip, and the respective first outputs from the plurality of regions of the analysis chip. Light is sequentially detected by the first detector, the plurality of second sub-light sources are sequentially turned on, the plurality of second beams are sequentially irradiated onto the plurality of regions of the analysis chip, and the analysis is performed. The biological material analysis method according to claim 6, wherein each second output light from the plurality of regions of the chip is sequentially detected by the second detector. The normal region is identified by irradiating the plurality of first beams to the plurality of regions of the analysis chip, respectively, and applying the first output light from the plurality of regions of the analysis chip to the first. The biological material analysis method according to claim 6 or 7 , wherein a region in which data within an average ± standard deviation of a plurality of data detected by the detector is detected as a normal region. The biological material analysis method according to any one of claims 6 to 8 , wherein the first detector is a zero-dimensional near-infrared sensor. The analysis chip includes a development layer for developing a specimen sample containing the biological material, and a reaction layer having a reagent that reacts with a test object in the biological material to generate a colored material. The biological material analysis method according to any one of 6 to 9 .

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