JP4050674B2 - Cell activator, whitening agent, hyaluronic acid production promoter, elastin production promoter, antioxidant, and external preparation for skin - Google Patents

Description

The present invention relates to a cell activator, a whitening agent, a hyaluronic acid production promoter, an elastin production promoter, an antioxidant, and a skin external preparation. More specifically, a cell activator, a whitening agent, a hyaluronic acid production promoter, an elastin production promoter, an antioxidant, containing one or more plant extracts selected from Brachythecaceae plants, and It relates to an external preparation for skin.

  Causes of aging symptoms such as wrinkles, stains, and decreased skin elasticity due to aging and ultraviolet rays, decreased cell function, melanin production and pigmentation due to ultraviolet rays, decrease and degeneration of dermal matrix components such as hyaluronic acid and elastin, ultraviolet rays, etc. Examples include oxidative damage of cells. In order to prevent and ameliorate such aging symptoms, search for various active ingredients and formulation studies have been conventionally conducted. As the cell activator, the essence of Ponkan (see Patent Document 1), as the whitening agent, the water and / or organic solvent extract of Hakutsuru Reishi (see Patent Document 2), and as the hyaluronic acid production promoter, Anaaaosa ( As an elastin production promoter, neem seed cell (see Patent Document 4) is known, and as an antioxidant, an extract of the Sargoceae family Sarogase (see Patent Document 5) is known.

  In addition, the prior art regarding a cell activator, a whitening agent, a hyaluronic acid production promoter, an elastin production promoter, an antioxidant, and a skin external preparation containing an extract of the plant belonging to the family Aonyuginaceae as an active ingredient was not recognized.

JP 2001-131045 A JP 2003-89630 A JP-A-6-9422 JP 2003-183151 A Japanese Patent Laid-Open No. 10-182413

  Conventional cell activators, whitening agents, hyaluronic acid production promoters, elastin production promoters, and antioxidants may be insufficient as essential effects, and the development of better active ingredients is expected. It had been. The present invention has been made in view of such conventional problems, and is naturally derived and highly safe, and has an excellent cell activation effect, whitening effect, hyaluronic acid production promoting effect, elastin production promoting effect, antioxidant An object of the present invention is to find an active ingredient having an action and to provide an external preparation for skin useful for preventing and improving aging.

  In order to solve the above-described problems, the present inventors have studied various naturally-derived substances with respect to actions useful for preventing and improving aging. As a result, we have found excellent cell activation, whitening, hyaluronic acid production, elastin production, and antioxidant activity in extracts of one or more plants selected from the plant of Aeginokeceae, and further investigation Repeatedly, the present invention has been completed. That is, the present invention relates to a cell activator, a whitening agent, a hyaluronic acid production promoter, an elastin production promoter, an antioxidant, and a skin, each of which contains an extract of one or two or more plants of the family Alanaceae. An external preparation is provided.

  ADVANTAGE OF THE INVENTION According to this invention, the cell activator, whitening agent, hyaluronic acid production promoter, elastin production promoter, and antioxidant which have the outstanding effect can be provided. In addition, by adding these to skin external preparations, skin external preparations and foods for improving aging prevention that exhibit excellent effects in the prevention and improvement of skin aging symptoms such as wrinkles, tarmi, skin firmness, stains, and scum The composition can be provided.

The plant used as a raw material of the present invention may be a plant of the family Brachythecaceae . The Aoginugoke plant belonging to the family, Aoginugoke genus (Brachythecium), Kayagoke genus (Rhynchostegium), Atsubusagoke genus (Homalothecium), Atsu Busa moss beetle species (Palamocladium), Yanonegoke genus (Bryhnia), Kiburinagigoke genus (Eurhynchium), Higebagoke genus (Cirriphyllum) , More than 20 genera are known, such as the genus Myocladada . The Aoginugoke plants of the genus, Aoginugoke (Brachythecium populeum), Arahahitsujigoke (Brachythecium brotheri), Kagiyanonegoke (Brachythecium uncinifolium), Kehitsujigoke (Brachythecium wichurae), Komanohitsujigoke (Brachythecium coreanum), Tanigoke (Brachythecium rivulare), Nagahitsujigoke (Brachythecium buchananii), Hanehitsujigoke (Brachythecium plumosum), Himohitsujigoke (Brachythecium helminthocladum), Hirohafusagoke (Brachytheciu m salebrosum ) and the like are known. The Kayagoke plants of the genus, Aohaigoke (Rhynchostegium riparioides), such as Kokayagoke (Rhynchostegium pallidifolium) is known. The Atsubusagoke plants of the genus, is known and Atsubusagoke (Homalothecium laevisetum), as the thickness Busa moss beetle genus plant, mediation Busa moss beetle (Palamocladium neilgheriense), etc. Hosohidagoke (Palamocladium macrostegium) is known. The Yanonegoke plants of the genus, Alaska Yano Ne Moss (Bryhnia hultenii), Kin Mo Yano Ne Moss (Bryhnia trichomitria), die Seth Yano Ne Moss (Bryhnia hultenii var. Cumbifolia), are known, such as Yanonegoke (Bryhnia novae-angilae) Yes. The Kiburinagigoke plants of the genus, Kiburinagigoke (Eurhynchium arbuscula), Tsukushinagigoke (Eurhynchium polystictum), such as horsetail Nagi moss beetle (Eurhynchium hians) is known.

  The plant used as a raw material for the present invention is not particularly limited as long as it is a plant belonging to the family Alanaceae, but for reasons such as availability and effectiveness, it is preferable to use a plant belonging to the genus Aoginoke and Papaver. I can do it. As the plant belonging to the genus Aoginoke, the plant belonging to the genus Amanuela, it is preferable to use A. elegans, Araha genus, Amanella, Aoinoke, etc., and the use of A. elegans is particularly preferable from the viewpoint of effectiveness.

  When using these Aonyuginaceae plants, it is common to use an extract. For extraction, any of the spores, spore bodies, and gametophytes of the plant of the family Alanaceae can be used, but for easy use, the whole plant of the spore body and gametophytes may be used. In the extraction, it may be used as it is, but considering the extraction efficiency, it is preferable to perform the extraction after performing processing such as shredding, drying, and pulverization. The extraction can be performed by immersing in an extraction solvent or by an extraction method using a supercritical fluid or a subcritical fluid. In order to increase the extraction efficiency, homogenization may be performed in stirring or an extraction solvent. The extraction temperature is suitably about 5 ° C. to the boiling point of the extraction solvent. The extraction time varies depending on the type of extraction solvent and the extraction temperature, but it is appropriate to set it to about 1 hour to 14 days.

  Extraction solvents include water, lower alcohols such as methanol, ethanol, propanol, and isopropanol, polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, dipropylene glycol, and glycerin, and ethers such as ethyl ether and propyl ether. , Solvents such as esters such as butyl acetate and ethyl acetate, and ketones such as acetone and ethyl methyl ketone can be used, and one or more of these are selected and used. Further, physiological saline, phosphate buffer, phosphate buffered saline, or the like may be used. Furthermore, you may use 1 type, or 2 or more types of supercritical fluids and subcritical fluids, such as water, a carbon dioxide, ethylene, propylene, ethanol, methanol, ammonia.

  Extracts from the above-mentioned solvents of Aeginokeceae can be used as they are, but decolorized and deodorized as long as the concentrated and dried solids are dissolved again in water or a polar solvent, or the physiological action is not impaired. It may be used after a purification treatment such as desalting or a fractionation treatment by column chromatography or the like. The above-mentioned extracts of Aoginophyceae plants, processed products and fractions thereof can be freeze-dried after each treatment and fractionation and dissolved in a solvent before use.

  The extract of Aonyugoraceae plant has excellent cell activation, whitening, hyaluronic acid production promotion, elastin production promotion, antioxidant activity, cell activator, whitening agent, hyaluronic acid production promoter, elastin production It can be used as an accelerator and an antioxidant.

  Although the cell activator which uses the extract of Aoginophyceae plant as an active ingredient exhibits the outstanding activation effect | action with respect to various cells, especially exhibits the outstanding effect with respect to a dermal fibroblast.

  A whitening agent containing an extract of the plant of Aeginokeceae as an active ingredient is effective in improving pigmentation symptoms such as stains and buckwheat, and is particularly effective in suppressing melanin production based on inhibition of tyrosinase activity.

  Hyaluronic acid production promoters and elastin production promoters, which are extracted from the plant of Aeginokeceae, are effective in promoting the production of dermal matrix components, and are effective in promoting the production of hyaluronic acid and elastin. To do.

  Antioxidants containing an extract from the plant of Aeginokeae as an active ingredient exhibit an excellent antioxidant effect, but in particular exhibit an excellent effect on free radical scavenging action.

  Moreover, the skin external preparation for anti-aging improvement which exhibits the effect excellent in prevention and improvement of a skin aging symptom can be obtained by mix | blending the extract of Aoginophyceae plant with a skin external preparation. Furthermore, the extract of the Alanaceae plant can also be used in foods and beverages for the purpose of maintaining health and providing nutrition.

  The compounding amount when blending the extract of the plant of Aoginodaceae into the skin external preparation can be adjusted according to the type of skin external preparation and the purpose of use, etc., but from the point of effect and stability, 0.0001-50.0 weight% is preferable, More preferably, it is 0.001-10.0 weight%.

  The dosage form of the external preparation for skin containing the extract of the plant of Aeginokeceae is arbitrary, and can be provided as a solubilizing system such as lotion, an emulsifying system such as cream or emulsion, or a dispersing system such as calamine lotion. Furthermore, it can also be provided in various dosage forms such as aerosols, ointments, powders and granules filled with a propellant.

  In addition, as for the external preparation for skin containing the extract of Aoginoke family plant, in addition to the extract of Aoginoke family plant, if necessary, it is usually a pharmaceutical, quasi-drug, skin cosmetic, hair cosmetic and washing. Oil components, moisturizers, powders, pigments, emulsifiers, solubilizers, cleaning agents, UV absorbers, thickeners, drugs, fragrances, resins, antibacterial and antifungal agents, alcohols, etc. It can mix | blend suitably. In addition, other cell activators, whitening agents, hyaluronic acid production promoters, elastin production promoters, and antioxidants can be used as long as the effects of the present invention are not impaired.

  In the following, the production examples of the extracts of the Alanaceae plant, the test for evaluating each action, the formulation example as a topical skin preparation, and the use test will be described in more detail, but the technical scope of the present invention is not limited thereby. It is not limited.

[Production Example 1]
10 kg of a 50 wt% aqueous ethanol solution was added to 1 kg of a dry pulverized whole plant of Aogynagoceae and immersed for 7 days at room temperature. The extract was collected by filtration, and after removing the solvent, the plant extract of the family Alanaceae was obtained.

[Production Example 2]
Nine liters of water was added to 1 kg of a dry pulverized whole plant of Aoginophyceae and extracted by refluxing at 90 ° C. for 6 hours. The extract was collected by filtration, and after removing the solvent, the plant extract of the family Alanaceae was obtained.

[Production Example 3]
Nine liters of methanol was added to 1 kg of a dry pulverized whole plant of Aoginophyceae and soaked at room temperature for 7 days. The extract was collected by filtration, and after removing the solvent, the plant extract of the family Alanaceae was obtained.

[Production Example 4]
The whole plant of the Alanaceae plant was introduced into a supercritical extraction apparatus, and extracted using a supercritical fluid of carbon dioxide at 40 ° C. under a pressure of 15 MPa. The extract was collected, and a plant extract of the Alanaceae family was obtained.

  Next, it shows about the activation effect | action of the dermal fibroblast of the Aoginoke department plant extract. The sample used was an extract of honey moss extracted from honey moss using Production Example 1.

The evaluation was performed according to the following procedure. Normal human dermal fibroblasts were seeded in a 96-well microplate at 2.0 × 10 ⁴ cells per well. The seeding medium used was Dulbecco's modified Eagle medium (DMEM) supplemented with 1% fetal bovine serum. After culturing for 24 hours, the culture medium was replaced with a test medium to which a sample having an arbitrary concentration was added, and further cultured for 48 hours. Next, the medium containing 400 μg / mL of 3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyltetrazolium bromide (MTT) was exchanged and cultured for 2 hours. Formazan produced by the opening of the tetrazolium ring was removed. Extraction was performed with 2-propanol, and absorbance at 550 nm was measured with a microplate reader. At the same time, the absorbance at 650 nm was measured as turbidity, and the cell activation effect was evaluated by the difference between the two measured values. The evaluation results are shown in Table 1 as relative values with the cell activation effect in the blank with no sample as 100. In the table, * and ** indicate a significance probability of less than 5% (P <0.05) with respect to the significance probability P value in the t test, and a significance probability of less than 1% (P <0.01). It is represented by **.

  As is clear from Table 1, a significant dermal fibroblast activation effect was observed in the medium supplemented with the honeydew extract. In particular, when 0.5 to 1.0 mg / mL of the honey oak moss extract was added, a significant dermal fibroblast activation effect was observed with a risk rate of less than 1% compared to the blank. From this, it was clarified that the honey sheep moss extract has an excellent dermal fibroblast activation effect.

  Next, the evaluation of the melanin production inhibitory effect of the Aoginoke department plant extract is shown. The sample used was an extract of honey moss extracted from honey moss using Production Example 1.

  The evaluation was performed according to the following procedure. B16 mouse melanoma F0 strain (B16F0) cells were seeded in a 35 mm dish at 2000 cells per dish. After culturing for 24 hours, the culture medium was replaced with Dulbecco's modified Eagle medium (DMEM) supplemented with 5 wt% fetal calf serum (FCS) to which a sample of an arbitrary concentration was added. After culturing for 7 days, cells were harvested using 0.25% trypsin, transferred to a 1.5 mL microtube and centrifuged to obtain a cell precipitate. Furthermore, instead of the medium to which the sample was added, Dulbecco's modified Eagle medium (DMEM) to which 5 wt% fetal calf serum (FCS) was added was used as a blank, and visual determination was similarly performed. Further, as shown in Table 2, the visual judgment was performed by a five-step evaluation. Table 3 shows the determination results.

  As is clear from Table 3, a significant melanin production inhibitory effect was observed when using a medium supplemented with 0.1-3 to 0.5 mg / mL of the honeydew extract. In particular, no blackening was observed when 0.5 mg / mL of the A. elegans extract was added. From this, it was clarified that the honey sheep moss extract has an excellent melanin production inhibitory action.

  Next, the evaluation of the tyrosinase activity inhibitory action of the plant extracts of the Alanaceae family is shown. The sample used was an extract of honey moss extracted from honey moss using Production Example 1.

The evaluation was performed according to the following procedure. Normal human epidermal melanocytes were seeded in a 96-well microplate so as to be 3.0 × 10 ⁴ cells per well. As a seeding medium, Medium154S manufactured by Kurabo Industries Co., Ltd. was used. After 24 hours, the medium was replaced with a sample solution adjusted to each concentration by Medium154S, and further cultured for 48 hours. Next, the cells were exchanged with 75 μL of 1 wt% Triton-X-containing phosphate buffer to completely lyse the cells. 50 μL of this was used as a crude enzyme solution, and 50 μL of 0.05 wt% L-dopa-containing phosphate buffer as a substrate was added thereto, and the mixture was allowed to stand at 37 ° C. for 2 hours. The absorbance at 405 nm immediately after the addition of the substrate and at the end of the reaction was measured with a microplate reader, and the amount of produced dopamelanin was measured. At the same time, the amount of protein in each sample was measured, and the amount of dopamelanin produced per amount of protein was calculated. As a tyrosinase activity inhibitory action, it showed in Table 4 by the relative value which set the dopamelanin production amount per protein amount in the blank without a sample as 100. In the table, * and ** indicate a significance probability of less than 5% (P <0.05) with respect to the significance probability P value in the t test, and a significance probability of less than 1% (P <0.01). It is represented by **.

  As is clear from Table 4, a significant tyrosinase activity inhibitory effect was observed when a medium supplemented with the honeydew extract 0.5 to 1.0 mg / mL was used. In particular, when 1.0 mg / mL of the honey oat moss extract was added, a significant tyrosinase activity inhibitory action was observed at a risk rate of less than 1% compared to the blank. From this, it was clarified that the honeydew extract has an excellent tyrosinase activity inhibitory action.

  Next, evaluation of the hyaluronic acid production promoting action of the plant family plant extract will be shown. The sample used was an extract of honey moss extracted from honey moss using Production Example 1.

The evaluation was performed according to the following procedure. Dulbecco's modified basal medium supplemented with 0.5% by weight fetal calf serum, in which normal human dermal fibroblasts are seeded in a 96-well microplate so as to be 2.0 × 10 ⁴ per well, and a sample of any concentration is added (DMEM) was cultured at 37 ° C. for 5 days, and the amount of hyaluronic acid in the culture supernatant was measured by the enzyme-linked immunosorbent assay (ELISA) method. At the same time, the number of fibroblasts was counted, the amount of hyaluronic acid produced per cell was calculated, and the relative value with the amount of hyaluronic acid produced per blank cell containing no sample as 100 was shown in Table 5.

  As is clear from Table 5, when 7.8-62.5 μg / mL of the honey oat moss extract was added, a significant hyaluronic acid production promoting action was observed as compared to the case where it was not added. In particular, when 15.6 to 62.5 μg / mL of the honeydew extract was added, a significant hyaluronic acid production promoting action was observed with a risk rate of less than 1%. From this, it was clarified that the honeydew extract has an excellent hyaluronic acid production promoting action.

  Next, evaluation of the elastin production promoting action of the plant family plant extract will be shown. The sample used was an extract of honey moss extracted from honey moss using Production Example 1.

The evaluation was performed according to the following procedure. Normal human dermal fibroblasts are seeded in a 96-well microplate at 2.0 × 10 ⁴ cells per well, and 1.0 wt% fetal bovine serum-added Dulbecco's basal medium supplemented with an arbitrary concentration of sample ( DMEM) was cultured at 37 ° C. for 24 hours, and the amount of elastin in the culture supernatant was measured by ELISA. At the same time, the number of fibroblasts was counted, and the amount of elastin produced per cell was calculated. Table 6 shows the relative values with the amount of elastin produced per blank cell containing no sample taken as 100.

  As is clear from Table 6, a significant elastin production-promoting effect was recognized when 0.025 to 0.1 mg / mL of the honey scorpion extract was added compared to the case where the extract was not added. In particular, when 0.05 to 0.1 mg / mL of the honeydew extract was added, a significant elastin production promoting effect was observed at a risk rate of less than 1%. From this, it was clarified that the honey sheep moss extract has an excellent elastin production promoting action.

  Next, it shows about the antioxidant effect of the Aonyuginaceae plant extract. The sample used was an extract of honey moss extracted from honey moss using Production Example 1.

The evaluation was performed according to the following procedure. 100 μL of a honey-mushroom extract solution diluted to an arbitrary concentration with a 50 wt% ethanol aqueous solution was added to a 96-well microplate. Next, 100 μL of a 1,1-diphenyl-2-picrylhydrazyl (DPPH) solution prepared with ethanol to a concentration of 0.2 mM was added to a 96-well microplate. The mixture was left in the dark with stirring, and the absorbance at 516 nm was measured after 24 hours. When the absorbance of the blank with no sample added was (A) and the absorbance when the sample was added was (B), the value of formula (1) was defined as the radical elimination rate. The evaluation results are shown in Table 7.
Formula (1) {1- (B) / (A)} × 100 (%)

  As is clear from Table 7, it was found that the A. elegans extract has an antioxidant effect.

  Then, the prescription example of the skin external preparation which mix | blended the Aogynagoceae plant extract which concerns on this invention is shown.

[Formulation Example 1] Emulsion (1) Squalane 10.0 (wt%)
(2) Methylphenylpolysiloxane 4.0
(3) Hydrogenated palm kernel oil 0.5
(4) Hydrogenated soybean phospholipid 0.1
(5) Polyoxyethylene monostearate
Sorbitan (20E.O.) 1.3
(6) Sorbitan monostearate 1.0
(7) Glycerin 4.0
(8) Methyl paraoxybenzoate 0.1
(9) Carboxyvinyl polymer 0.15
(10) Purified water 53.85
(11) Arginine (1 wt% aqueous solution) 20.0
(12) Honey sheep moss extract [Production Example 1] 5.0
Production method: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. After emulsification, start cooling and add (11) and (12) sequentially and mix uniformly.

[Prescription Example 2] Lotion (1) Ethanol 15.0 (% by weight)
(2) Polyoxyethylene (40E.O.) hydrogenated castor oil 0.3
(3) Fragrance 0.1
(4) Purified water 78.38
(5) Citric acid 0.02
(6) Sodium citrate 0.1
(7) Glycerin 1.0
(8) Hydroxyethyl cellulose 0.1
(9) Honey sheep moss extract [Production Example 3] 5.0
Production method: (2) and (3) are dissolved in (1). After dissolution, (4) to (8) are sequentially added, and then sufficiently stirred, (9) is added and mixed uniformly.

[Prescription Example 3] Cream (1) Squalane 10.0 (% by weight)
(2) Stearic acid 2.0
(3) Hydrogenated palm kernel oil 0.5
(4) Hydrogenated soybean phospholipid 0.1
(5) Cetanol 3.6
(6) Lipophilic glyceryl monostearate 2.0
(7) Glycerin 10.0
(8) Methyl paraoxybenzoate 0.1
(9) Arginine (20% by weight aqueous solution) 15.0
(10) Purified water 40.7
(11) Carboxyvinyl polymer (1% by weight aqueous solution) 15.0
(12) Honey sheep moss extract [Production Example 1] 1.0
Production method: The oil phase components (1) to (6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 80 ° C. The oil phase component is added to this while stirring and uniformly emulsified with a homogenizer. After the emulsification is completed, add (11), start cooling, add (12) at 40 ° C., and mix uniformly.

[Formulation Example 4] Cosmetic liquid (1) Purified water 27.45 (% by weight)
(2) Glycerin 10.0
(3) Sucrose fatty acid ester 1.3
(4) Carboxyvinyl polymer (1% by weight aqueous solution) 17.5
(5) Sodium alginate (1 wt% aqueous solution) 15.0
(6) Polyglyceryl monolaurate 1.0
(7) Macadamia nut oil fatty acid phytosteryl 3.0
(8) N-lauroyl-L-glutamic acid di (phytosteryl-2-octyldodecyl) 2.0
(9) Hardened palm oil 2.0
(10) Squalane (from olive) 1.0
(11) Behenyl alcohol 0.75
(12) Beeswax 1.0
(13) Jojoba oil 1.0
(14) 1,3-butylene glycol 10.0
(15) L-arginine (10% by weight aqueous solution) 2.0
(16) Honey sheep moss extract [Production Example 1] 5.0
Production method: The aqueous phase components (1) to (6) are mixed and dissolved by heating at 75 ° C. On the other hand, the oil phase components (7) to (14) are mixed and dissolved by heating at 75 ° C. Next, the oil phase component is added to the aqueous phase component and preliminary emulsification is performed, followed by uniform emulsification with a homomixer. Cooling is started after completion of emulsification, and (15) is added at 50 ° C. Cool further to 40 ° C, add (16) and mix evenly.

[Formulation Example 5] Aqueous gel (1) Carboxyvinyl polymer 0.5 (% by weight)
(2) Purified water 86.7
(3) Sodium hydroxide (10% by weight aqueous solution) 0.5
(4) Ethanol 10.0
(5) Methyl paraoxybenzoate 0.1
(6) Fragrance 0.1
(7) Honey sheep moss extract [Production Example 4] 2.0
(8) Polyoxyethylene (60E.O.) hydrogenated castor oil 0.1
Manufacturing method: (1) is added to (2), and after stirring uniformly, (3) is added. After stirring uniformly, add (5) previously dissolved in (4). After stirring uniformly, the previously mixed (6) to (8) are added and stirred and mixed uniformly.

[Formulation Example 6] Cleansing Fee (1) Squalane 81.0 (wt%)
(2) Polyoxyethylene glyceryl isostearate 15.0
(3) Purified water 3.0
(4) Honey sheep moss extract [Production Example 4] 1.0
Manufacturing method: (1) and (2) are uniformly dissolved. (3) and (4) are sequentially added to this and mixed uniformly.

[Prescription Example 7] Face-wash foam (1) Stearic acid 16.0 (% by weight)
(2) Myristic acid 16.0
(3) Lipophilic glyceryl monostearate 2.0
(4) Glycerin 20.0
(5) Sodium hydroxide 7.5
(6) Palm oil fatty acid amidopropyl betaine 1.0
(7) Purified water 36.5
(8) Honey sheep moss extract [Production Example 3] 1.0
Production method: The oil phase components (1) to (4) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (5) to (7) are heated and dissolved at 80 ° C., and mixed and stirred uniformly with the oil phase components. Cooling is started, and (8) is added at 40 ° C. and mixed uniformly.

[Prescription Example 8] Make-up base cream (1) Squalane 10.0 (% by weight)
(2) Cetanol 2.0
(3) Glycerin tri-2-ethylhexanoate 2.5
(4) Lipophilic glyceryl monostearate 1.0
(5) Propylene glycol 11.0
(6) Sucrose fatty acid ester 1.3
(7) Purified water 69.4
(8) Titanium oxide 1.0
(9) Bengala 0.1
(10) Yellow iron oxide 0.4
(11) Fragrance 0.1
(12) Honey sheep moss extract [Production Example 2] 1.2
Production method: The oil phase components (1) to (4) are mixed and dissolved by heating at 75 ° C. On the other hand, the aqueous phase components (5) to (7) are mixed and dissolved by heating at 75 ° C., and the pigments (8) to (10) are added thereto and dispersed uniformly with a homomixer. The oil phase component is added to the water phase component and emulsified with a homomixer. Cooling is started after the emulsification, and the components (11) and (12) are added at 40 ° C. and mixed uniformly.

[Formulation Example 9] Emulsion foundation (1) Methylpolysiloxane 2.0 (wt%)
(2) Squalane 5.0
(3) Octyldodecyl myristate 5.0
(4) Cetanol 1.0
(5) Polyoxyethylene (20E.O.)
Sorbitan monostearate 1.3
(6) Sorbitan monostearate 0.7
(7) 1,3-butylene glycol 8.0
(8) Xanthan gum 0.1
(9) Methyl paraoxybenzoate 0.1
(10) Purified water 57.4
(11) Titanium oxide 9.0
(12) Talc 7.4
(13) Bengala 0.5
(14) Yellow iron oxide 1.1
(15) Black iron oxide 0.1
(16) Fragrance 0.1
(17) Honey sheep moss extract [Production Example 4] 1.0
Production method: The oil phase components (1) to (6) are mixed and dissolved by heating at 75 ° C. On the other hand, the aqueous phase components (7) to (10) are mixed and dissolved by heating at 75 ° C., and the pigments (11) to (15) are added thereto and uniformly dispersed with a homomixer. Add oil phase ingredients and emulsify. Cooling is started after the emulsification is completed, and components (16) and (17) are sequentially added at 40 ° C. and mixed uniformly.

[Formulation Example 10] Water-in-oil emollient cream (1) Liquid paraffin 30.0 (% by weight)
(2) Microcrystalline wax 2.0
(3) Vaseline 5.0
(4) Diglycerin oleate 5.0
(5) Sodium chloride 1.3
(6) Potassium chloride 0.1
(7) Propylene glycol 3.0
(8) 1,3-butylene glycol 5.0
(9) Methyl paraoxybenzoate 0.1
(10) Honey sheep moss extract [Production Example 1] 1.0
(11) Purified water 47.4
(12) Fragrance 0.1
Production method: Dissolve (5) and (6) in a part of (11) to 50 ° C., and gradually add to (4) heated to 50 ° C. with stirring. After mixing this, it disperse | distributes uniformly to (1)-(3) heated and melted at 70 degreeC. (7) to (10) are added to the remainder of (11) heated and dissolved at 70 ° C. while stirring and emulsified with a homomixer. Cooling is started after completion of emulsification, and (12) is added at 40 ° C. and mixed uniformly.

[Prescription Example 11] Pack (1) Purified water 58.9 (% by weight)
(2) Polyvinyl alcohol 12.0
(3) Ethanol 17.0
(4) Glycerin 5.0
(5) Polyethylene glycol (average molecular weight 1000) 2.0
(6) Honey sheep moss extract [Production Example 2] 5.0
(7) Fragrance 0.1
Production method: (2) and (3) are mixed, heated to 80 ° C, and then dissolved in (1) heated to 80 ° C. After uniformly dissolving, add (4) and (5), and start cooling while stirring. Cool to 40 ° C, add (6) and (7) and mix uniformly.

[Prescription Example 12] Bath agent (1) Fragrance 0.3 (% by weight)
(2) Honey sheep moss extract [Production Example 1] 1.0
(3) Sodium bicarbonate 50.0
(4) Sodium sulfate 48.7
Production method: (1) to (4) are mixed uniformly.

[Prescription Example 13] Hair wax (1) Stearic acid 3.0 (% by weight)
(2) Microcrystalline wax 2.0
(3) Cetyl alcohol 3.0
(4) Highly polymerized methylpolysiloxane 2.0
(5) Methylpolysiloxane 5.0
(6) Poly (oxyethylene / oxypropylene)
Methylpolysiloxane copolymer 1.0
(7) Methyl paraoxybenzoate 0.1
(8) 1,3-butylene glycol 7.5
(9) Arginine 0.7
(10) Purified water 73.6
(11) Honey sheep moss extract [Production Example 1] 2.0
(12) Fragrance 0.1
Production method: The oil phase components (1) to (6) are mixed and heated and dissolved at 75 ° C. On the other hand, the aqueous phase components (7) to (10) are dissolved by heating at 75 ° C., the oil phase component is added, and the mixture is emulsified with a homomixer. Cooling is started after the emulsification, and the components (11) and (12) are added at 40 ° C. and mixed uniformly.

[Prescription Example 14] Hair artic (1) Ethanol 50.0 (% by weight)
(2) Purified water 48.9
(3) Honey sheep moss extract [Production Example 3] 1.0
(4) Fragrance 0.1
Production method: Components (1) to (4) are mixed and homogenized.

  Next, a use test was conducted using a prescription containing a plant extract of Aoginoke department, and the improvement effect was evaluated for wrinkles, tarmi, skin firmness, spots, and kusumi. At that time, each of the extracts of the plant belonging to the family Alanaceae listed in Table 8 was added to the formulation of the emulsion shown in Formulation Example 1, and the use test was conducted as Examples 1-6. Moreover, the Aoginoke department plant extract was replaced with purified water, and a use test was simultaneously conducted as Comparative Example 1.

  For each sample, each group of 20 male and female panelists in their 40s to 60s who have noticeable symptoms such as wrinkles, tarmi, skin firmness, spots, and kusumi are used as a group for 2 months. Changes in skin condition were observed and evaluated. As indicators of skin symptoms, wrinkles, tarmi, skin firmness, stains, and kusumi were evaluated in three stages: “Improved”, “Slightly improved”, and “No change”. Showed.

  From Table 9, for wrinkles, tarmi, skin firmness, stains, and kusumi, in the comparative example use group that does not contain the plant extract of Aoginoke department, 60% or more of panelists did not improve, In the example use group in which the product was blended, 60% or more of panelists clearly improved.

  As described above, in the examples of the present invention, the effects of improving wrinkles, tarmi, skin firmness, spots, and stains were superior to those of the conventional comparative example. From this, it became clear that the skin external preparation which mix | blended the Aoginoke department plant extract exhibits the effect excellent in prevention and improvement of a skin aging symptom.

Claims (4)

A cell activator comprising an extract of Brachythelium plumos as an active ingredient. A hyaluronic acid production promoter characterized by comprising an extract of honey sheep moss ( Brachitheplum plumosum ) as an active ingredient. An elastin production promoter characterized by comprising an extract of Brachythelium plumosum as an active ingredient. A skin external preparation for improving aging prevention, comprising an extract of honey sheep moss ( Brachythecium plumosum ) as an active ingredient.