JP3386353B2 - Conductive nucleic acid polymer thin film - Google Patents
Conductive nucleic acid polymer thin filmInfo
- Publication number
- JP3386353B2 JP3386353B2 JP34038097A JP34038097A JP3386353B2 JP 3386353 B2 JP3386353 B2 JP 3386353B2 JP 34038097 A JP34038097 A JP 34038097A JP 34038097 A JP34038097 A JP 34038097A JP 3386353 B2 JP3386353 B2 JP 3386353B2
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- acid polymer
- thin film
- conductive
- polymer thin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 229920000642 polymer Polymers 0.000 title claims description 49
- 108020004707 nucleic acids Proteins 0.000 title claims description 47
- 102000039446 nucleic acids Human genes 0.000 title claims description 47
- 150000007523 nucleic acids Chemical class 0.000 title claims description 47
- 239000010409 thin film Substances 0.000 title claims description 40
- 239000000758 substrate Substances 0.000 claims description 9
- 230000003287 optical effect Effects 0.000 claims description 5
- 239000007787 solid Substances 0.000 claims description 3
- 238000005266 casting Methods 0.000 claims 1
- 239000000975 dye Substances 0.000 description 13
- 239000000138 intercalating agent Substances 0.000 description 10
- 229920000831 ionic polymer Polymers 0.000 description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- 108020004414 DNA Proteins 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 239000004094 surface-active agent Substances 0.000 description 6
- 238000010586 diagram Methods 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 4
- 239000010408 film Substances 0.000 description 4
- 239000007864 aqueous solution Substances 0.000 description 3
- 238000009830 intercalation Methods 0.000 description 3
- 230000002687 intercalation Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000011259 mixed solution Substances 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- -1 quaternary ammonium salt compounds Chemical class 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 238000000018 DNA microarray Methods 0.000 description 1
- DPKHZNPWBDQZCN-UHFFFAOYSA-N acridine orange free base Chemical compound C1=CC(N(C)C)=CC2=NC3=CC(N(C)C)=CC=C3C=C21 DPKHZNPWBDQZCN-UHFFFAOYSA-N 0.000 description 1
- 239000008346 aqueous phase Substances 0.000 description 1
- DZBUGLKDJFMEHC-UHFFFAOYSA-N benzoquinolinylidene Natural products C1=CC=CC2=CC3=CC=CC=C3N=C21 DZBUGLKDJFMEHC-UHFFFAOYSA-N 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 244000309466 calf Species 0.000 description 1
- 150000004657 carbamic acid derivatives Chemical class 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 125000002091 cationic group Chemical group 0.000 description 1
- 239000003093 cationic surfactant Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 229960005542 ethidium bromide Drugs 0.000 description 1
- 238000007306 functionalization reaction Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 125000001165 hydrophobic group Chemical group 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 239000010445 mica Substances 0.000 description 1
- 229910052618 mica group Inorganic materials 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 239000010453 quartz Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 210000001541 thymus gland Anatomy 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 229910052724 xenon Inorganic materials 0.000 description 1
- FHNFHKCVQCLJFQ-UHFFFAOYSA-N xenon atom Chemical compound [Xe] FHNFHKCVQCLJFQ-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E10/00—Energy generation through renewable energy sources
- Y02E10/50—Photovoltaic [PV] energy
- Y02E10/549—Organic PV cells
Landscapes
- Light Receiving Elements (AREA)
Description
【０００１】[0001]
【発明の属する技術分野】この出願の発明は、導電性核
酸ポリマー薄膜とその応用に関するものである。さらに
詳しくは、この発明は、各種の電気回路等に有用な光導
電性の核酸ポリマー薄膜と、この薄膜を組み込んだ光応
答性電気回路等に関するものである。TECHNICAL FIELD The invention of this application relates to a conductive nucleic acid polymer thin film and its application. More specifically, the present invention relates to a photoconductive nucleic acid polymer thin film useful for various electric circuits and the like, and a photoresponsive electric circuit incorporating the thin film.
【０００２】[0002]
【従来の技術】従来より、ＤＮＡやＲＮＡ等の核酸ポリ
マーの性質の一つとして、隣接塩基対間にカチオン性色
素が挿入するインターカレーション現象が知られてい
る。この出願の発明者等は、このような界面活性色素
（インターカレーター）を核酸ポリマー水溶液上に展開
して核酸ポリマー・インターカレーター単分子膜を形成
し、この膜の表面圧を測定することによって、水溶液中
の核酸ポリマーの含量、一本鎖核酸ポリマー／プローブ
のハイブリダイゼーションの有無、および核酸ポリマー
の塩基配列を識別する方法を発明し、既に特許出願して
いる（特開平８−３１３５２１号公報）。また、この出
願の発明者等は、核酸ポリマーとカチオン性界面活性剤
との混合によるポリイオンコンプレックスを所定のパタ
ーンで薄膜化する方法を発明し、既に特許出願している
（特願平９−２１４７７号）。2. Description of the Related Art An intercalation phenomenon in which a cationic dye is inserted between adjacent base pairs has been known as one of the properties of nucleic acid polymers such as DNA and RNA. The inventors of the present application develop a surface active dye (intercalator) on a nucleic acid polymer aqueous solution to form a nucleic acid polymer intercalator monomolecular film, and measure the surface pressure of the film. A method for identifying the content of the nucleic acid polymer in the aqueous solution, the presence / absence of hybridization of the single-stranded nucleic acid polymer / probe, and the base sequence of the nucleic acid polymer has been invented and has already applied for a patent (JP-A-8-313521). . The inventors of the present application have invented a method for forming a thin film of a polyion complex by mixing a nucleic acid polymer and a cationic surfactant in a predetermined pattern, and have already filed a patent application (Japanese Patent Application No. 9-21477). issue).
【０００３】しかしながら、この出願の発明者等も含
め、界面活性色素をインターカレートした核酸ポリマー
やその薄膜が、核酸ポリマー本来の特性をどのように変
化させるかについて報告した例は存在しない。一方、生
体材料を利用し、様々な電気回路やデバイスを作成する
試みが提案されている。例えば、酵素等を用いるものと
しては、その優れた分子識別機能を利用して化学物質を
計測するセンサーや、その生化学反応を発光反応に導く
ことによるフォトカウンターやフォトデバイスなどのバ
イオセンサーが知られている。また、酵素等の蛋白質を
トランジスターの上に薄膜状に固定化した半導体バイオ
センサーやバイオチップモデル、あるいはスイッチング
機能や増幅機能などを有する蛋白質を検索し、これらの
蛋白質を回路中に組み込んだバイオトランジスターも提
案されている。However, there is no example including the inventors of the present application, which reports on how a nucleic acid polymer intercalated with a surface-active dye or a thin film thereof changes the original properties of the nucleic acid polymer. On the other hand, attempts have been proposed to create various electric circuits and devices using biomaterials. For example, as a sensor that uses an enzyme or the like, a sensor that measures a chemical substance by utilizing its excellent molecular recognition function and a biosensor such as a photocounter or a photodevice that guides the biochemical reaction into a luminescence reaction are known. Has been. In addition, we searched for semiconductor biosensors and biochip models in which proteins such as enzymes were immobilized in a thin film on transistors, or proteins with switching and amplification functions, and searched for biotransistors incorporating these proteins in the circuit. Is also proposed.
【０００４】しかしながら、ＤＮＡやＲＮＡ等の核酸ポ
リマーを利用したバイオセンサーやバイオトランジスタ
ーは、これまでに全く知られていない。However, no biosensor or biotransistor using a nucleic acid polymer such as DNA or RNA has been known so far.
【０００５】[0005]
【発明が解決しようとする課題】この出願の発明者等
は、界面活性色素をインターカレートした二重らせん核
酸ポリマーを薄膜状に単離し、その特性を検討した結
果、本来は絶縁性を示す核酸ポリマーが、界面活性色素
のインターカレーションにより光導電性を獲得すること
を見出した。そして、そのような導電性は、照射光の強
度や波長により変化することも見出した。DISCLOSURE OF THE INVENTION The inventors of the present application isolated a double-helix nucleic acid polymer in which a surface-active dye is intercalated into a thin film, and studied the characteristics of the polymer, and as a result, it showed that it was originally insulating. It was found that the nucleic acid polymer acquires photoconductivity by intercalation of the surface active dye. It was also found that such conductivity changes depending on the intensity and wavelength of irradiation light.
【０００６】この出願の発明は、発明者等によるこのよ
うな新規な知見に基づいてなされたものであって、バイ
オセンサーやバイオトランジスター等の材料として有用
な導電性核酸ポリマー薄膜と、この薄膜を用いた光セン
サー、光応答性トランジスター等を提供することを目的
としている。[0006] The invention of this application was made based on such novel findings by the inventors, and is a conductive nucleic acid polymer thin film useful as a material for biosensors, biotransistors, and the like, and the thin film It is intended to provide an optical sensor, a photoresponsive transistor and the like used.
【０００７】[0007]
【課題を解決するための手段】この出願は、上記の課題
を解決する発明として、界面活性色素をインターカレー
トさせて二重らせん核酸ポリマーを一対の電極を有する
基板上にキャストしてなる基板一体化固体薄膜であっ
て、光照射によって導電性となることを特徴とする導電
性核酸ポリマー薄膜を提供する。As an invention for solving the above-mentioned problems, this application has a double-helix nucleic acid polymer having a pair of electrodes in which a surface-active dye is intercalated.
Provided is a substrate-integrated solid thin film cast on a substrate , which is made conductive by light irradiation, and is provided as a conductive nucleic acid polymer thin film.
【０００８】またこの出願は、一対のくし型電極を有す
る基板上に一体化された上記の導電性核酸ポリマー薄膜
を提供し、そして、上記の導電性核酸ポリマー薄膜を回
路中に有し、光照射によって通電性となることを特徴と
する光応答性電気回路を提供する。さらにこの出願は、
上記の光応答性電気回路と、この回路の通電により駆動
する表示手段とを有することを特徴とする光センサーを
提供する。さらにまた、この出願は、上記の導電性核酸
ポリマー薄膜を回路中に有し、照射光の波長および／ま
たは強度に応じて電流強度を変化させることを特徴とす
る光応答性トランジスターをも提供する。This application also has a pair of comb electrodes.
Conductive nucleic acid polymer thin film integrated on a substrate
And a photoresponsive electric circuit comprising the above-mentioned conductive nucleic acid polymer thin film in a circuit, which becomes conductive by light irradiation. Furthermore, this application
There is provided an optical sensor including the above-mentioned photoresponsive electric circuit and a display unit driven by energization of the circuit. Furthermore, this application also provides a photoresponsive transistor characterized by having the above-mentioned conductive nucleic acid polymer thin film in a circuit and changing the current intensity according to the wavelength and / or intensity of irradiation light. .
【０００９】以下、これらの各発明について、実施の形
態を詳しく説明する。Embodiments of each of these inventions will be described in detail below.
【００１０】[0010]
【発明の実施の形態】この発明の導電性核酸ポリマー薄
膜は、例えば、図１に例示したような方法で作成するこ
とができる。すなわち、水層の気水界面に界面活性色素
インターカレーター溶液と界面活性剤を展開し、この水
相に二本鎖ＤＮＡまたはＲＮＡからなる二重らせん核酸
ポリマーを添加する。インターカレーターは正電荷を持
つため、負電荷を持つ核酸ポリマーと気水界面でポリイ
オンコンプレックスを形成し、薄膜状となる。もちろ
ん、予め核酸ポリマーを水層中に添加して核酸ポリマー
溶液とし、この溶液の気水界面にインターカレーター溶
液と界面活性剤を展開するようにしてもよい。BEST MODE FOR CARRYING OUT THE INVENTION The conductive nucleic acid polymer thin film of the present invention can be produced, for example, by the method illustrated in FIG. That is, a surfactant dye intercalator solution and a surfactant are developed on the air-water interface of an aqueous layer, and a double helix nucleic acid polymer composed of double-stranded DNA or RNA is added to this aqueous phase. Since the intercalator has a positive charge, it forms a polyion complex at the air-water interface with the nucleic acid polymer having a negative charge to form a thin film. Of course, the nucleic acid polymer may be added in advance to the aqueous layer to prepare a nucleic acid polymer solution, and the intercalator solution and the surfactant may be developed at the air-water interface of this solution.
【００１１】界面活性剤としては、適宜なものを選択し
て使用することができ、例えば、第４級アンモニウム塩
化合物やアミン化合物、それらの塩、カルバミン酸塩化
合物等が挙げられる。色素インターカレーターとして
は、核酸ポリマーの塩基対間へのインターカレーション
現象が知られているアクリジンオレンジや臭化エチジウ
ム等の公知の色素に対して疎水基修飾して界面活性を付
与したものを使用する。また、この出願の発明者等によ
る先願発明（特開平８−３１３５２１号公報）に開示さ
れている次式の界面活性色素インターカレーターを使用
することもできる。As the surfactant, an appropriate one can be selected and used, and examples thereof include quaternary ammonium salt compounds, amine compounds, salts thereof, and carbamate compounds. As the dye intercalator, use is made of known dyes such as acridine orange and ethidium bromide, which are known to have intercalation between base pairs of nucleic acid polymers, and which have been surface-modified with hydrophobic groups. To do. Further, the interfacial dye intercalator represented by the following formula, which is disclosed in a prior application (Japanese Patent Laid-Open No. 8-313521) by the inventors of this application, can also be used.
【００１２】[0012]
【化１】 [Chemical 1]
【００１３】[0013]
【化２】 [Chemical 2]
【００１４】このようにして形成した核酸ポリマー薄膜
は、例えば、表面を圧縮し、水層内に挿入した基板に移
し取ることによって単離することができる。乾燥させ、
基板から分離することによって、この発明の導電性核酸
ポリマー薄膜が得られる。また、基板と一体化したまま
の状態であってもよい。基板としては、ガラス、雲母、
石英、シリコン、炭素、各種の金属、無機物、有機樹脂
等を適宜に選択して用いることができる。また、基板の
形状も板材等の固形形状だけではなく、フィルム等の可
塑形状とすることもできる。さらに、例えば１対の幅広
くし型電極等に一体化することもできる。The nucleic acid polymer thin film thus formed can be isolated by, for example, compressing the surface and transferring to a substrate inserted in the aqueous layer. Dried
The conductive nucleic acid polymer thin film of the present invention is obtained by separating from the substrate. Further, it may be in a state of being integrated with the substrate. As the substrate, glass, mica,
Quartz, silicon, carbon, various metals, inorganic substances, organic resins and the like can be appropriately selected and used. Further, the shape of the substrate is not limited to a solid shape such as a plate material, but may be a plastic shape such as a film. Further, it can be integrated with, for example, a pair of wide electrode.
【００１５】また、この発明の核酸ポリマー薄膜は、実
施例に示したように、上記混合溶液を遠心して、ポリイ
オンコンプレックスを沈殿物として回収し、水洗し、乾
燥させて得ることもできる。以上のとおりにして製造さ
れた核酸ポリマー薄膜は、後述する実施例に示すよう
に、光照射によって導電性を獲得する。そして、このよ
うな光導電性薄膜を使用することによって、光応答性電
気回路を作成することができる。The nucleic acid polymer thin film of the present invention can also be obtained by centrifuging the above mixed solution to recover the polyion complex as a precipitate, washing with water and drying, as shown in the examples. The nucleic acid polymer thin film produced as described above acquires conductivity by light irradiation, as shown in Examples described later. Then, by using such a photoconductive thin film, a photoresponsive electric circuit can be created.
【００１６】図２は、このような電気回路の一例を示し
た模式図である。この例の場合には、導電性核酸ポリマ
ー薄膜（１）は１対のくし型電極（２）にキャストさ
れ、電極（２）には電源（Ｖ）および電流計（Ａ）を接
続して回路が形成されている。各々の電極（２）は非接
触であるが、光（可視光）が照射されると核酸ポリマー
薄膜（１）が導電性となって、電極（２）間が電気的に
接触状態となり、回路は通電性となる。FIG. 2 is a schematic diagram showing an example of such an electric circuit. In the case of this example, the conductive nucleic acid polymer thin film (1) is cast on a pair of comb-shaped electrodes (2), and a power source (V) and an ammeter (A) are connected to the electrodes (2) to form a circuit. Are formed. Although the electrodes (2) are not in contact with each other, when the light (visible light) is irradiated, the nucleic acid polymer thin film (1) becomes conductive, and the electrodes (2) are electrically contacted with each other, so that the circuit Becomes conductive.
【００１７】このような電気回路は、例えば、回路の通
電により駆動する表示手段（例えば電流計、ランプ等）
を接続することにより、核酸ポリマー薄膜（１）への光
照射の有無を検出するための光センサーとして利用する
ことができる。そして、この発明の導電性核酸ポリマー
薄膜は、微量のＤＮＡやＲＮＡを用いて極微少形状とし
て作成することができるため、センサーのプローブ部分
を極小型化することができる。Such an electric circuit is, for example, display means (for example, ammeter, lamp, etc.) driven by energization of the circuit.
Can be used as an optical sensor for detecting the presence or absence of light irradiation on the nucleic acid polymer thin film (1). Since the conductive nucleic acid polymer thin film of the present invention can be formed in a very small shape using a small amount of DNA or RNA, the probe portion of the sensor can be made extremely small.
【００１８】さらに、この発明の導電性核酸ポリマー薄
膜は、バイオトランジスターにも応用することができ
る。図３はこのようなトランジスター構成の一例を示し
た模式図である。すなわち、この発明の導電性核酸ポリ
マー薄膜（１）をソース（Ｓ）とドレイン（Ｄ）に接続
して回路を形成し、薄膜に光（Ｌ）を照射すると、回路
を流れる電流（ｉ）は光（Ｌ）の強度と波長により変化
する。増幅や発振等も可能であり、全く新しい光応答性
トランジスターが可能となる。Furthermore, the conductive nucleic acid polymer thin film of the present invention can be applied to a biotransistor. FIG. 3 is a schematic diagram showing an example of such a transistor configuration. That is, when the conductive nucleic acid polymer thin film (1) of the present invention is connected to the source (S) and the drain (D) to form a circuit and the thin film is irradiated with light (L), the current (i) flowing through the circuit is It changes depending on the intensity and wavelength of light (L). Amplification, oscillation, etc. are also possible, enabling a completely new photo-responsive transistor.
【００１９】以下、実施例を示してこの出願の発明につ
いてさらに詳細かつ具体的に説明するが、この発明は以
下の例によって限定されるものではない。Hereinafter, the invention of this application will be described in more detail and specifically with reference to Examples, but the invention is not limited to the following examples.
【００２０】[0020]
【実施例】ウシ胸腺ＤＮＡの水溶液（33mg／50ml純水）
に脂質（界面活性剤）と両親媒性色素インターカレータ
ーを添加し（２Ｃ16Ｎ+ ２Ｃｌ- ：Ｃ18ＡＯ+ Ｉ- ＝
９：１）（28mg／50ml純水）、室温で混合し、混合溶液
を遠心することによって生成したポリイオンコンプレッ
クスを回収した。これを水洗し、乾燥して、ＤＮＡ−脂
質ポリイオンコンプレックスを得た。Example: Aqueous solution of calf thymus DNA (33 mg / 50 ml pure water)
Was added lipids (surfactant) an amphiphilic dye intercalator (2C 16 N + 2Cl -: C 18 AO + I - =
9: 1) (28 mg / 50 ml pure water) were mixed at room temperature, and the polyion complex produced by centrifuging the mixed solution was recovered. This was washed with water and dried to obtain a DNA-lipid polyion complex.
【００２１】なお、界面活性剤としては次式で表される
２Ｃ16Ｎ+ ２Ｃｌ- を用いた。2C 16 N + 2Cl − represented by the following formula was used as the surfactant.
【００２２】[0022]
【化３】 [Chemical 3]
【００２３】また、両親媒性色素インターカレーター
は、次式で表されるＣ18ＡＯ+ Ｉ- を用いた。As the amphipathic dye intercalator, C 18 AO + I − represented by the following formula was used.
【００２４】[0024]
【化４】 [Chemical 4]
【００２５】次いで、このポリイオンコンプレックスの
クロロホルム溶液（1 mg／10ml）を１対のくし型電極
（長さ２mm；電極ギャップ100 μm ) にキャストして、
この発明の核酸ポリマー薄膜（膜厚約10μm ）を作成し
た。電極には電源および電流計を接続し、電源から所定
の電圧を印加して、460nmのカットフィルターを通した5
00Wキセノンランプ光を薄膜に照射し、電極間を流れる
光電流を計測した。Then, a chloroform solution (1 mg / 10 ml) of this polyion complex was cast on a pair of comb electrodes (length 2 mm; electrode gap 100 μm),
A nucleic acid polymer thin film (film thickness of about 10 μm) of the present invention was prepared. A power source and an ammeter were connected to the electrodes, a predetermined voltage was applied from the power source, and the light was passed through a 460 nm cut filter.
The thin film was irradiated with 00W xenon lamp light, and the photocurrent flowing between the electrodes was measured.
【００２６】結果は図４および図５に示したとおりであ
る。図４に示したとおり、光照射によって電極間に電流
が流れることが確認された。また、図５に示したとお
り、印加する電圧に依存して光電流も変化することも確
認された。さらに、一定電圧を印加した場合であって
も、照射光の強度または波長を変えることによって電流
値が変化することも確認された。The results are shown in FIGS. 4 and 5. As shown in FIG. 4, it was confirmed that a current flows between the electrodes by light irradiation. Moreover, as shown in FIG. 5, it was also confirmed that the photocurrent also changed depending on the applied voltage. Furthermore, it was also confirmed that the current value changes by changing the intensity or wavelength of the irradiation light even when a constant voltage is applied.
【００２７】[0027]
【発明の効果】以上詳しく説明したとおり、この発明に
より、光照射によって導電性となる薄膜材料が提供され
る。これにより、光応答性のバイオセンサーやバイオト
ランジスターの開発が可能となる。As described above in detail, the present invention provides a thin film material which becomes conductive when irradiated with light. This enables the development of photoresponsive biosensors and biotransistors.
【図１】核酸ポリマーと色素インターカレターのポリイ
オンコンプレックスの形成状態を示した模式図である。FIG. 1 is a schematic diagram showing a formation state of a polyion complex of a nucleic acid polymer and a dye intercalator.
【図２】この発明の導電性核酸ポリマー薄膜を用いた光
応答性電気回路の一例を示した模式図である。FIG. 2 is a schematic diagram showing an example of a photoresponsive electric circuit using the conductive nucleic acid polymer thin film of the present invention.
【図３】この発明の導電性核酸ポリマー薄膜を用いた光
応答性トランジスターの一例を示した模式図である。FIG. 3 is a schematic view showing an example of a photoresponsive transistor using the conductive nucleic acid polymer thin film of the present invention.
【図４】図２に示した電気回路による光電流の計測結果
を示したグラフ図である。FIG. 4 is a graph diagram showing a photocurrent measurement result by the electric circuit shown in FIG.
【図５】図２に示した電気回路による光電流の別の計測
結果を示したグラフ図である。FIG. 5 is a graph showing another measurement result of photocurrent by the electric circuit shown in FIG.
１ 導電性核酸ポリマー薄膜 ２ くし型電極 Ｖ 電源 Ａ 電流計 Ｌ 光 Ｓ ソース Ｄ ドレイン 1 Conductive nucleic acid polymer thin film 2 comb electrodes V power supply A ammeter L light S source D drain
フロントページの続き (56)参考文献 特開 平８−313521（ＪＰ，Ａ) 特開 平10−219008（ＪＰ，Ａ) 下村政嗣，”ＤＮＡの二次元配列と光 機能化”，高分子，第46巻５月号，第 334頁，（1997) (58)調査した分野(Int.Cl.7，ＤＢ名) H01B 1/00 - 5/16 C07H 1/00 - 23/00 ＪＩＣＳＴファイル（ＪＯＩＳ) ＣＡ（ＳＴＮ) ＲＥＧＩＳＴＲＹ（ＳＴＮ)Continuation of the front page (56) References JP-A-8-313521 (JP, A) JP-A-10-219008 (JP, A) Shigemura Masatsugu, "Two-dimensional array of DNA and optical functionalization", Polymer, No. 1 Volume 46 May issue, p. 334, (1997) (58) Fields investigated (Int.Cl. 7 , DB name) H01B 1/00-5/16 C07H 1/00-23/00 JISST file (JOIS) CA (STN) REGISTRY (STN)
Claims (5)
二重らせん核酸ポリマーを一対の電極を有する基板上に
キャストしてなる基板一体化固体薄膜であって、光照射
によって導電性となることを特徴とする導電性核酸ポリ
マー薄膜。1. A double-helix nucleic acid polymer having a surface-active dye intercalated on a substrate having a pair of electrodes.
A substrate-integrated solid thin film formed by casting , which is made conductive by light irradiation, and is a conductive nucleic acid polymer thin film.
化された請求項１の導電性核酸ポリマー薄膜。2. The conductive nucleic acid polymer thin film according to claim 1, which is integrated on a substrate having a pair of comb-shaped electrodes .
薄膜を回路中に有し、光照射によって導電性となること
を特徴とする光応答性電気回路。3. A photoresponsive electric circuit comprising the conductive nucleic acid polymer thin film according to claim 1 or 2 in a circuit, which becomes conductive when irradiated with light.
路の通電により駆動する表示手段とを有することを特徴
とする光センサー。4. An optical sensor comprising the photoresponsive electric circuit according to claim 3 and display means driven by energization of the circuit.
薄膜を回路中に有し、照射光の波長および／または強度
に応じて電流強度を変化させることを特徴とする光応答
性トランジスター。5. A photoresponsive transistor comprising the conductive nucleic acid polymer thin film according to claim 1 or 2 in a circuit, and changing the current intensity according to the wavelength and / or intensity of irradiation light.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP34038097A JP3386353B2 (en) | 1997-12-10 | 1997-12-10 | Conductive nucleic acid polymer thin film |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP34038097A JP3386353B2 (en) | 1997-12-10 | 1997-12-10 | Conductive nucleic acid polymer thin film |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH11176238A JPH11176238A (en) | 1999-07-02 |
| JP3386353B2 true JP3386353B2 (en) | 2003-03-17 |
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ID=18336403
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|---|---|---|---|
| JP34038097A Expired - Fee Related JP3386353B2 (en) | 1997-12-10 | 1997-12-10 | Conductive nucleic acid polymer thin film |
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| JP (1) | JP3386353B2 (en) |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001181295A (en) * | 1999-12-24 | 2001-07-03 | Asahi Denka Kogyo Kk | Method for producing formed article |
| JP3469886B2 (en) | 2001-06-25 | 2003-11-25 | 独立行政法人通信総合研究所 | Light control electromagnetic wave circuit |
| JP4007000B2 (en) | 2001-12-25 | 2007-11-14 | 富士ゼロックス株式会社 | Conductive organic polymer |
| US9019685B2 (en) * | 2010-04-19 | 2015-04-28 | National Institute For Materials Science | Inductor comprising arrayed capacitors |
| JP6664809B2 (en) * | 2015-12-25 | 2020-03-13 | 国立研究開発法人物質・材料研究機構 | Spiral capacitor-inductor device |
-
1997
- 1997-12-10 JP JP34038097A patent/JP3386353B2/en not_active Expired - Fee Related
Non-Patent Citations (1)
| Title |
|---|
| 下村政嗣，"ＤＮＡの二次元配列と光機能化"，高分子，第４６巻５月号，第３３４頁，（１９９７） |
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