JP3173805B2 - Fructooligosaccharide-containing syrup and method for producing the same - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to a fructooligosaccharide-containing syrup having a favorable flavor and excellent storage stability.
And its manufacturing method .

[0002]

2. Description of the Related Art Fructooligosaccharides have a sweetness close to that of sucrose, are low in calories, are hardly carious, and are effective for selective growth of lactic acid bacteria and bifidobacteria having an intestinal action. It has recently been used as a seasoning in various foods and beverages.

[0003] The above fructooligosaccharides can be obtained by reacting sucrose with fructosyltransferase. When glucose is represented by G and fructose is represented by F, the production mechanism of fructooligosaccharide is GF first.
The β-1,2 bond between G and F of sucrose having the structure of sucrose is cleaved, and then the fructose F is transferred to sucrose GF to become GFF, hereinafter GFF
It is considered that fructooligosaccharides having a large number of saccharides, such as -F and GFFFF, are successively generated.

[0004] Granulated sugar having a high degree of purification is exclusively used as a raw material of sucrose. The sugar content of granulated sugar is 98.8% in Brix degree, and the sucrose content is 9
9.9% by weight, and the ash content in 100 g of granulated sugar is 0%.
g, calcium content is 0 mg.

[0005]

However, conventional fructooligosaccharides produced from granulated sugar as a raw material are monotonous as sweeteners, and thus have a limitation that they do not always meet diversifying tastes.

[0006] In addition, fructooligosaccharides produced from granulated sugar as raw materials, when in a syrup state, undergo acidification during the distribution and storage processes, and for example, those having a pH of 5.0 to 5.5 at the time of shipment. , PH in half a year
PH is about 3.5 in about one year. Especially after opening the container containing the syrup, the acidification proceeds even faster. And as acidification progresses, considerable amounts of fructooligosaccharides are hydrolyzed and decomposed into glucose and fructose, so they are not harmful to the human body, but the produced glucose and fructose are not lactic acid bacteria such as Escherichia coli but other than lactic acid bacteria and bifidobacteria. Since it has a growth activity even for intestinal general-purpose bacteria, there is a problem that the selective growth activity for lactic acid bacteria and bifidobacteria is relatively reduced.

The present inventor has already disclosed in Japanese Patent Application No. 58-13 / 1983.
No. 4661 (JP-A-60-27395).
The genus Aureobasidium is cultivated by liquid culture to obtain cultured cells or a culture solution containing the cells, and the cultured cells or the culture solution containing the cells are added to or contact-reacted with the sucrose solution to react the cells. Sucrose is reduced to 1- by fructosyltransferase, an intracellular enzyme contained in the body.
An application has been filed for a method of converting to inulin-type fructooligosaccharides containing ketose and nystose as main components.As with other fructooligosaccharide manufacturers, the use of a source other than granulated sugar as a sucrose source has been transferred to fructooligosaccharides. We did not consider it enough because we predicted that the efficiency would be worse.

[0008] The present invention has been found while studying the use of a substance other than granulated sugar as a sucrose source, and it has a flavor-preferable syrup containing fructooligosaccharides which is hardly acidified during storage . Yo
And a method for producing the same.

[0009]

The syrup containing fructooligosaccharides of the present invention is a sucrose made from sugarcane.
With a sugar content of 78-86% by weight and a reducing sugar content of 2-7% by weight
Sugar and a brown sugar, sugar stage before refined sugar is
The sucrose content is about 95% by weight and the reducing sugar content is 2-3.
Mineral content selected from the group consisting of tri-sweet sugar in weight%
Fructo at least part of the sucrose content of sugar containing
It is enzymatically converted to fructooligosaccharide by siltransferase

[0010] The method for producing a fructooligosaccharide-containing syrup of the present invention comprises the step of preparing a sucrose component prepared from sugarcane.
Is 78 to 86% by weight and the reducing sugar content is 2 to 7% by weight.
That brown sugar, raw sugar and Shrewsbury is a sugar of the previous stage of refined sugar
The sucrose content is about 95% by weight and the reducing sugar content is 2-3% by weight.
% Minerals selected from the group consisting of
The method is characterized in that at least a part of sucrose is converted into fructooligosaccharide by causing fructosyltransferase to act on an aqueous solution of sucrose.

Hereinafter, the present invention will be described in detail.

In the present invention, brown sugar , crude sugar , or trisaccharide is used as a sucrose source.

In the classification by the production method, sugar is divided into molasses and molasses depending on whether or not molasses is centrifuged during the production process. If we categorize this further,
According to Table 2.1 on page 30 of "Commodity Knowledge Series, Practical Knowledge of Confectionery, Published by Toyo Keizai Shimpo, Date of Issue: December 1, 1983", it is as shown on the left below, Large Dictionary 3, Compact Edition, Published by Kyoritsu Publishing Co., Ltd., Compact Edition 26
According to page 843 of "Issuance of printing: October 15, 1981", it is as shown on the right below.

The above-mentioned brown sugar (brown sugar) , crude sugar, and trisaccharide are indicated by #, ##, and ###, respectively.

[0015] sugar sugar ◆ beet sugar ◆Beet sugar  ◇ Crude sugar ## ◇ Red sugar △ Raw sugar ◇ Brown sugar# △ Medium sugar ◇ Shirashita sugar 赤 Red sugar 分 Mitsuto sugar 分 Cultivated white sugar ◇ Direct sugar sugar ◇ White sugar △ Cultivated white sugar △ Granulated sugar ・ Car sugar △ Car sugar ・ White grain ◇ Purified sugar △ Raw sugar ## △ White sugar ・Red Zara △ Granulated Sugar ・ Medium Zara △ Medium Soup ・ Car Sugar △ Car Sugar ◇ Refined Sugar ・ Kami White △ Car Sugar ・ Medium White ・ Kami White ・ San White ### △ Liquid sugar △ White Zara ・ Sucrose type ・ Slump type ・ Inverted sugar type ・ Granulated sugar ・ Mixed sugar type △ Powder sugar △ Glass sugar △ Kaku (sugar) sugar △ Kakuto sugar △ Ice (sand) sugar △ Powder sugar △ Granular sugar ◆Moist sugar  △ Brown sugar# △ White sugar △ Brown sugar △ Regenerated sugar

Examples of the beet sugar include brown sugar (brown sugar), brown sugar (brown sugar), and white sugar. Among them, the present invention
Brown sugar used in is a molasses-containing cane sugar made from sugar cane, the color because it is not purified is brown.
Although the sugar content is as low as 86.0% in Brix (98.8% for granulated sugar), it is characterized by its unique flavorful sweetness and high hygroscopicity. Sucrose content is, for example, 78-8
The content of the reducing sugar is, for example, about 2 to 7% by weight, and the water content is about 5 to 8% by weight. Ash content in brown sugar 100g is about 3.6g, calcium content is about 240mg,
Also contains iron and other minerals. However, these compositions vary considerably depending on the place of production and the manufacturing process. Since brown sugar contains relatively large amounts of organic substances and minerals other than sucrose, it has a higher nutritional value than granulated sugar.

[0017] Crude sugar is a sugar in a stage before it is refined, and its flavor is close to white sucrose, and it is suitable for boiled foods because it has "koku".

[0018] Trisucrose (caramel sugar) is a kind of sugar.
“Three warm” indicates the degree of whiteness. By the way, white zara has five temperatures and Kamibaku has four temperatures. The sugar content of trisaccharide is about 92.6%, the sucrose content is about 95% by weight, the reducing sugar content is 2-3% by weight, and contains about 0.4% by weight of protein. Ash content is about 0.2g and calcium content is about 30mg
It is. However, these compositions slightly vary depending on the place of production and the production process.

In the present invention, fructosyltransferase is allowed to act on an aqueous solution of brown sugar , crude sugar , or trisaccharide to convert at least a part of sucrose enzymatically into fructooligosaccharide. About 70% of sucrose
As described above, 80% or more changes to fructo-oligosaccharide represented by fructosylnystose, nystose, 1-ketoose, and glucose as a by-product.

Examples of the fructosyltransferase include molds such as Aureobasidium, Aspergillus, Penicillium, Fusarium, Greosporium, Saccharomyces, Rhodotorula, Pichia, Hansenula, Candida and the like. Enzymes of microbial origin and enzymes of plant origin such as Jerusalem artichoke and asparagus are used. Of these, fructosyltransferase of fungi belonging to the genus Aureobasidium is particularly important. The fungus of the genus Aureobasidium is FERM-P No. 4257
The strain is registered as ATCC No. 20524.

When a fructosyltransferase derived from a microorganism is used, the microorganism is cultured in a medium containing sucrose as a carbon source, and the enzyme contained in the cells separated from the culture solution and the enzyme contained in the culture filtrate obtained by separating the cells are used. Or enzymes contained in a culture solution containing cells.

As the medium composition, for example, rice bran 0.1 to 1
Wt%, vitamin C 0.01-0.4 wt%, sucrose
When 0.5 to 5% by weight is used and culture is conducted with aeration and stirring for 24 hours or more, a culture solution containing active fructosyltransferase cells can be obtained. When this is liquid-cultured using sucrose as a carbon source, sucrose in the medium is converted into fructooligosaccharides, and cells containing fructosyltransferase are mainly produced in the cells, which can be used as a crude enzyme. .

The enzymatic action of the cells containing fructosyltransferase is most active at a pH of 5 to 6 and a temperature of 55 to 60 ° C.

The fructooligosaccharide-containing syrup obtained by the method of the present invention is useful as a seasoning for foods and drinks, and is also useful as feed for animals such as livestock.

[0025]

[Action] While a conventional fructooligosaccharide-containing syrup using granulated sugar as a sucrose source is acidified with time and converted to glucose and fructose,
If you use brown sugar , raw sugar or brown sugar as a sucrose source,
Acidification over time is effectively suppressed. This is brown sugar ,
It is presumed that organic substances other than sucrose and minerals contained in the crude sugar or trisaccharide are used as a buffer to suppress fluctuations in pH.

[0026]

The present invention will be further described with reference to the following examples.

Example 1 <Purification of Enzyme> 2 kg of a bacterial cell containing fructosyltransferase of Aureobasidium pullulans was disrupted in a cell membrane, and fructosyltransferase was released into 30 liters of water. At this time, the titer of fructosyltransferase was adjusted to 100 units / ml to obtain a crude fructosyltransferase aqueous solution. One unit is a titer that produces 1 micromol of 1-ketoose per minute in an aqueous sucrose solution at a temperature of 50 ° C. and a concentration of 50% by weight.

Next, the above-mentioned aqueous solution of crude fructosyltransferase was subjected to ultrafiltration with a molecular weight cutoff of 20,000.
Concentrate to 1.5 liters, titer 2000 units / ml,
Subsequently, water was added to the aqueous solution of fructosyltransferase to adjust the titer to 6 liters and the titer to 500 units / ml, and then ultrafiltration was repeated to add 1.5 liters of the aqueous solution of fructosyltransferase and a titer of 2000 units / ml.
And concentrated. As a result, a purified fructosyltransferase aqueous solution was obtained.

<Production of fructooligosaccharides> Brown sugar (sugar content: 86.0%, sucrose content: 80% by weight, reducing sugar content: 5.5% by weight, ash content: 3.6 g in 100 g of brown sugar,
100 kg of calcium (240 mg) was dissolved in 40 liters of water, and 0.5 kg of the purified fructosyltransferase aqueous solution having a titer of 2,000 units / ml obtained above was added thereto.
The mixture was stirred at 5 ° C. for 24 hours, and sterilized at 100 ° C. after the completion of the reaction.

<Analysis Results> The results of analysis of the solid content in the fructooligosaccharified brown sugar syrup, which is the reactant, are as shown in Table 1 below .

[0031]

[Table 1]  Raw brown sugar syrup  Polysaccharides 6.0 wt% 6.0 wt% Fructosyl Nistose * 0.0 wt% 2.0 wt% Nistose * 0.0 wt% 14.5 wt% 1-Kestose * 0.5 wt% 33.5 wt% Sucrose 89.5 wt% 16.2 wt% Glucose 2.0 wt% 25.8 wt%Fructose 2.0 wt% 2.0 wt%

Fructosyl nystose, nystose and 1-kestose indicated by * are fructooligosaccharides, and the total amount is 50.0% by weight.

<Change over time> The fructooligosaccharide-containing brown sugar syrup obtained above was placed in a polypropylene container, sealed, and allowed to stand in a room to measure the pH over time. The results are as shown in Table 2 below , and it can be seen that the pH is stable even after long-term storage.

[0034]

[Table 2]

Comparative Example 1 <Production of fructooligosaccharide> 100 kg of granulated sugar was dissolved in 40 liters of water, and 0.25 kg of a separately prepared purified fructosyltransferase aqueous solution having a titer of 2000 units / ml was added at 55 ° C.
The mixture was stirred for 4 hours, and sterilized at 100 ° C. after the completion of the reaction.

<Analysis Results> The analysis results of the solid content in the fructooligosaccharide-granulated sugar syrup, which is the reaction product, are as shown in Table 3 below .

[0037]

[Table 3]  Raw granulated sugar syrup  Polysaccharides, etc. 26.7 wt%Fructose 0.1 wt% 2.5 wt%

Fructosyl nystose, nystose and 1-kestose indicated by * are fructooligosaccharides, and the total amount is 57.7% by weight.

<Change over time> The change over time of the fructooligosaccharide-granulated sugar syrup obtained above was examined in the same manner as in Example 1. The results are as shown in Table 4 below. Unlike the results of Example 1, p
The change with time of H was large.

[0040]

[Table 4]

Example 2 <Production of fructooligosaccharide> 100 kg of crude sugar (raw sugar) (sucrose content: 98.0% by weight, reducing sugar content: 0.7% by weight) was dissolved in 40 liters of water.
Add 0.3 kg of the purified fructosyltransferase aqueous solution having a titer of 2000 units / ml obtained in Example 1 and add 55 kg.
The mixture was stirred at 24 ° C. for 24 hours, and sterilized at 100 ° C. after the completion of the reaction.

<Analysis Results> The analysis results of the solid components in the fructooligosaccharide-modified crude sugar syrup, which is the reaction product, are as shown in Table 5 below .

[0043]

[Table 5]  Raw sugar syrup  Polysaccharides 0.6 wt% 0.6 wt% Fructosyl nistose * 0.0 wt% 2.4 wt% Nistose * 0.0 wt% 19.0 wt% 1-Kestose * 0.7 wt% 34.4 wt% Sucrose 98.0 wt% 14.0 wt% Glucose 0.4 wt% 27.4 wt%Fructose 0.3 wt% 2.2 wt%

Fructosyl nystose, nystose and 1-kestose indicated by * are fructooligosaccharides, and the total amount is 55.8% by weight.

<Time-dependent change> The time-dependent change of the crude fructooligosaccharide syrup obtained above was examined in the same manner as in Example 1. The results are shown in Table 6 below.
This shows that the pH is stable even after long-term storage.

[0046]

[Table 6]

Example 3 <Production of fructooligosaccharides> Trisucrose (trisaccharide of carbohydrates) (sugar content 92.6%) was used as a sucrose source.
%, Sucrose content 95.0% by weight, reducing sugar content 2.9% by weight, ash content 0.2g, calcium content 30 in triglyceride 100g
Example 1 was repeated except that the above was used, and the change over time was examined in the same manner as in Example 1 with respect to the produced fructooligosaccharide-treated trisaccharide syrup. The pH was constant at 5.4 from immediately after production to one year later. Value was kept.

[0048]

The fructooligosaccharide-containing syrup of the present invention has a different flavor from conventional fructooligosaccharides produced using granulated sugar as a raw material and contains nutrients, especially minerals, so that it can meet diversifying tastes. And get the nutrients you need.

The fructooligosaccharide- containing composition of the present invention
In lops , sucrose contained therein is converted to fructooligosaccharides regardless of whether the brown sugar , raw sugar or trisaccharide used as a raw material has a higher nutritional value than granulated sugar, Low calorie can be achieved.

Further, the fructooligosaccharide-containing syrup obtained according to the present invention has excellent stability over time, so that it is hardly acidified even after long-term storage, and has little conversion to glucose and fructose. Therefore, the quality is stable in the distribution process and the storage process after production, and the stability is maintained even after opening. Therefore, the sweetness does not change and the selective growth activity against useful enteric bacteria such as lactic acid bacteria and bifidobacteria is maintained.

Claims (2)

(57) [Claims] 1. The sucrose component made from sugarcane is
Reducing sugar content in 78 to 86% by weight is 2 to 7 wt% <br/> brown sugar, a sugar stage before refined sugar raw sugar and holidays
Loin content is about 95% by weight and reducing sugar content is 2-3% by weight
Furakutoshiruto at least part of the sucrose content of <br/> sugar containing minerals selected from the group consisting of three brown sugar is
Enzymatically fructooligosaccharides containing syrup was converted to fructooligosaccharides by lance Feller zero. 2. A sucrose component made from sugarcane.
Reducing sugar content in 78 to 86% by weight is 2 to 7 wt% <br/> brown sugar, a sugar stage before refined sugar raw sugar and holidays
Loin content is about 95% by weight and reducing sugar content is 2-3% by weight
Three by the action of fructosyltransferase in an aqueous solution of <br/> sugars including temperature minerals selected from the group consisting of sugars, and characterized by converting at least a portion of the sucrose content in fructooligosaccharides is For producing fructooligosaccharide syrup.