JP2604908Y2 - Bacteria detection instrument - Google Patents

Description

[Detailed description of the invention]

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a bacterium detecting device capable of quickly and easily measuring the number of bacteria in a sample without requiring specialized skills or knowledge. The present invention has been developed in the field of metal processing,
It can be effectively used in a wide range of fields such as the dye field and the food field where spoilage is a problem.

[0002]

2. Description of the Related Art Conventionally, in the field of urine testing, the number of bacteria in a sample such as urine has been measured in order to discover inflammation and to estimate a disease state. For example, if bacteria in the urine multiply, it is diagnosed as bacteriuria, suggesting a urinary tract infection. Therefore, the number of bacteria is measured for early treatment and drug administration. In such a case, the measurement of the number of bacteria is performed on most of the samples by a plate culture method, and is often performed using a commercially available kit. More specifically, for example, a predetermined amount of a sample is cultured on an agar plate medium or the like at about 37 ° C. for about 24 hours, the number of generated colonies is determined, and the number of bacteria is measured from the number of colonies. ing. Furthermore, with respect to urinary bacteria considered to be particularly important, the pathogens are currently identified using various commercially available kits. However, in these methods, even if the doctor collects urine of the patient, the test result is known the next day. Therefore, there is a problem that a prompt and appropriate emergency treatment according to the medical condition cannot be performed, for example, the treatment is delayed due to waiting for the test result, and the medical condition worsens. In addition, patients cannot receive treatment on the same day, and the time or economic burden is large. In addition, these methods require special techniques and specialized knowledge, and have a problem that measurement cannot be performed without a cultivation facility.

[0003] As a method for quickly and easily detecting bacteria in urine, there are a nitrite test method, a catalase test method, and a trace urine sugar test method (all of which are described in "Urine Test Manual", written by Ichita Yoshizawa, published by Ichiyakuyaku) , Published in 1991). First, the nitrite test method is to detect bacteria by filter paper, utilizing the fact that nitrate in urine is reduced to nitrite by some kind of bacteria, and this nitrite is subjected to a grease reaction. It is a method of detecting. However, the nitrite test method has a disadvantage that bacteria having no nitrate reducing activity cannot be detected. Second, the catalase test method has a problem in that the reagent is unstable and has low specificity. Furthermore, the micro urinary sugar test method has a problem that Pseudomonas aeruginosa and deformed bacteria do not react.

For this reason, there has been proposed a method for detecting bacteria using a negatively charged filter (Japanese Patent Laid-Open No. 1-1).
24767). In this method, urine is diluted with a strong acid such as a dilute hydrochloric acid solution and then applied to a negatively charged filter (dropping).
Adsorb, then dye by dropping safranin, and after staining,
The diluted hydrochloric acid solution is dropped to perform primary washing, then the diluted hydrochloric acid solution is dropped to perform secondary washing, and judgment is made based on color strength. However, in this method, when adsorbing microorganisms to the filter, it is necessary to perform a pretreatment operation with a strong acid having a pH of 1 to 3, and it is necessary to take care that a strong acid such as diluted hydrochloric acid does not touch the skin of the measurer. There is a disadvantage that the operation is extremely complicated, for example, a secondary washing operation is required to remove excess free dye. This method is not a detection method unique to bacteria, because animal cells such as leukocytes are also adsorbed and stained.

Further, in the presence of EDTA, urine and the like are adsorbed to a positively charged semi-permeable membrane epoxy-glass fiber filter having a pore size of 0.65 μm, and then stained with safranin.
Thereafter, a method of washing with a detergent having a pH of 2.7 to 4.0 and measuring the number of viable microorganisms based on the color of the bacteria adsorbed on the filter has been proposed (JP-B-56-38).
196, U.S. Pat. No. 4,336,337). However, in the case of this method, addition of a chelating agent is essential for dyeing. In addition, this method requires a vacuum pump or a suction device to remove excess dye,
There are drawbacks that the device becomes large-scale and the place where it is used is limited. That is, this method
It can be implemented only in large hospitals equipped with facilities, and practically difficult in small hospitals or clinics that occupy the majority in Japan. This method is also not a detection method specific to bacteria, because animal cells such as leukocytes are also adsorbed and stained.

[0006] As a solution to the conventional disadvantage, the present applicant has proposed a method of collecting microorganisms on a hydrophobic filter, then staining the microorganisms, and measuring the number of microorganisms in a sample from the degree of coloring of the microorganisms. (Japanese Unexamined Patent Publication No. Hei 4-2)
No. 18392). According to this method, it is possible to measure the number of microorganisms in a sample quickly and easily without requiring any skilled technique, specialized knowledge, or special equipment. However, when actually used, there is a problem that the degree of coloring is slightly low, and it takes time for determination.
Further, in practice, there has been a strong demand for a bacterium detection instrument itself that can measure the number of microorganisms in a sample quickly and easily without requiring expert skills, specialized knowledge, and special equipment. .

[0007] The present invention solves such conventional drawbacks, does not require special equipment and specialized knowledge and skills, and is quick (within 30 seconds) and easily provided with bacteria in a sample. It is an object of the present invention to provide a bacteria detection instrument capable of measuring the number.

[0008]

That is, the present invention provides a first aspect.
An opening 1 is provided in the upper part, a connection port 2 for a suction means is provided in a side part, and a substantially cylindrical base part A in which a water absorbing material 3 is placed is penetrated vertically in a central part. A bacterium collecting / collecting device having a tubular portion 4, a hydrophobic filter 5 attached to the center of the upper surface of the tubular portion 4, and a color contrast figure 6 stuck around the hydrophobic filter 5. Detector B
And a bacteria sample introduction part C having a funnel-shaped introduction hole 7 in the center and a pre-filter 8 mounted in the introduction hole 7. It is an object of the present invention to provide a bacterium detection instrument, wherein the detection section B is configured such that the bacterium sample introduction section C can be sequentially coupled to the bacterium collection / detection section B.

Further, the present invention secondly has a substantially cylindrical base portion having an opening 1 at an upper portion, a connection port 2 for suction means on a side portion, and a water absorbing material 3 placed inside. A and
A bacteria collection / detection unit B having a plurality of tubular portions 4 penetrating vertically and having a hydrophobic filter 5 attached so as to cover at least the upper surface of the tubular portion 4; The introduction hole 7 is provided circumferentially, and a second introduction hole 7 ′ is provided at an intermediate position on the circumference between the plurality of introduction holes 7 and an adjacent introduction hole 7. And a bacteria sample introduction part C in which a pre-filter 8 is attached to the introduction hole 7. The bacteria collection / detection part B is provided on the base part A. The bacterial sample introduction part C is arranged so that the bacterial sample introduction part C can be sequentially coupled thereto, and the tubular part 4 and the introduction hole 7 are arranged so as to be linear in the vertical direction. Bacteria detection characterized by being rotatable in the left-right direction with respect to the base part A. The equipment is provided.

Hereinafter, the first and second bacteria detection devices of the present invention will be described with reference to the drawings. FIG. 1 is a central longitudinal sectional view showing one embodiment of the first bacteria detection device of the present invention, and (a).
Shows a state of being fitted and integrated, and (b) shows a state of being disassembled. FIG. 2 is a plan view of the bacteria collecting / detecting section B in FIG. FIG. 3 is a longitudinal sectional view at the center showing another embodiment of the first bacteria detection device of the present invention. FIG. 4 is a perspective view showing one embodiment of the second bacteria detection device of the present invention, FIG. 5 is a side view thereof, and FIG. 6 is a plan view thereof. FIG. 7 is a sectional view taken along the line II of FIG. 6, and FIG. 8 is a sectional view taken along the line II in FIG. FIG. 9 is a sectional view taken along a line.
FIG. 7 is a sectional view taken along the line II-II of FIG. 6.

Each of the first and second bacteria detection devices of the present invention basically includes a base A, a bacteria collection / detection unit B, and a bacteria sample introduction unit C.

First, the first bacteria detection device of the present invention will be described. First, the base part A has an opening 1 at an upper part and a connection port 2 with a suction means at a side part,
It has a substantially cylindrical shape with a water absorbing material 3 placed inside. The connection port 2 is connected to a suction unit (for example, a suction pump, a syringe, or the like) via a connection unit such as a pipe prepared separately as needed. In addition, a cloth,
It is preferable that a mist adsorbent 9 made of cotton, polyethylene, or the like is installed in consideration of bacterial contamination. Further, it is preferable to provide a wide supporting portion 10 below the base portion A in order to secure stability. As the water absorbing material 3, filter paper, gauze, absorbent cotton, non-woven fabric, or the like may be used.

Next, the bacteria collecting / detecting section B has a tubular portion 4 vertically penetrating at the center, and a hydrophobic filter 5 is mounted at the center of the upper end of the tubular portion 4. As shown in FIG. 2, a color contrast figure 6 is attached around the hydrophobic filter 5. Note that the bacteria detection device shown in FIG. 1 and the bacteria detection device shown in FIG. 3 are almost the same except that the shape of the bacteria collection / detection section B is different. That is, in FIG. 3, the surface opposite to the surface on which the color contrast FIG. 6 is pasted is not planar but has a cavity. This bacteria collection
The detecting portion B is generally provided with a step portion 11 for coupling (usually fitting) to the base portion A as shown in the drawing. Further, in order to prevent scattering of a dye solution or a washing solution introduced from a bacterial sample introduction part C described later, the lower end of the tubular part 4 penetrating vertically is fitted to the bottom of the base part A when fitted to the base part A. It is preferable to reach near. Further, the upper surface of the bacteria collecting / detecting unit B is circular and flat, and a hydrophobic filter 5 is mounted at the center of the upper surface, that is, at the upper end of the tubular portion 4. In addition, a hydrophobic filter supporting member 12 supporting the hydrophobic filter 5 is provided between the hydrophobic filter 5 and the upper part of the tubular portion 4.
Is provided. This hydrophobic filter support member 12
Usually, a member made of a plastic member (eg, a polyethylene member) having a large number of ventilation holes is used.

The hydrophobic filter 5 used in the present invention
Is suitable as a filter for collecting bacteria because it has weak or no polarity and can easily remove excess dye. On the other hand, a polar filter is not preferable because it is not easy to remove excess dye. Examples of such a hydrophobic filter include a hydrophobic filter such as a nylon filter, a fluorine filter such as polytetrafluoroethylene (tetrafluoroethylene resin), a polyolefin filter such as polypropylene, and a glass filter. Among these filters, a hydrophobic filter made of polytetrafluoroethylene or polypropylene is particularly preferable because an excessive dye can be easily removed. If a hydrophilic material, for example, a nitrocellulose filter is made hydrophobic by surface treatment, it can be used as a hydrophobic filter. Here, the surface treatment is coating or the like, and as the material used for the coating, the above-mentioned nylon-based, fluorine-based, or polyolefin-based material can be used.

The filtration pore size of the hydrophobic filter 5 for collecting bacteria may be appropriately selected according to the kind of the target bacteria, but is usually about 1 to 4 μm.
Considering the collection rate and filterability, those having a thickness of about 2 to 3 μm are preferable.

The size of the suction filtration area (colored area) of the hydrophobic filter 5 for collecting bacteria is not particularly limited as long as it is easy to see. The shape of the portion to be suction-filtered is not particularly limited, but a circular shape is preferable in view of visibility, and its caliber (diameter)
Is preferably about 2 to 6 mm in a circular shape. The size of the whole hydrophobic filter 5 for collecting bacteria is not particularly limited. The shape of the hydrophobic filter 5 as a whole is preferably circular from the point of viewability, and its diameter (diameter) is 10 to 1 mm.
It is preferably about 5 mm.

As the color of the hydrophobic filter 5 for collecting bacteria, a color that can be easily determined may be determined in consideration of the dye to be used. In order to easily determine the degree of coloring, a white hydrophobic filter is preferable. Also, a transparent or translucent hydrophobic filter can be used.

In the bacteria collecting / detecting section B, a color contrast figure 6 is attached circumferentially around the hydrophobic filter 5. FIG. 6 shows a color control using a sample having a known number of bacteria, for example, Japanese Patent Application Laid-Open No. 4-218392 and Japanese Patent Application No. 5-1219.
By taking a color photograph of a hydrophobic filter for collecting bacteria which has been stained and washed by the method described in the specification of No. 63,
Alternatively, it can be produced by coloring a filter paper or the like to the same extent as the stained hydrophobic filter for collecting bacteria, or by printing a similar color on paper.

For example, when measuring the number of bacteria in urine, a color contrast chart is prepared as follows. That is,
Low concentration of urinary bacteria in healthy human urine [1ml
(About 10,000 per milliliter)]. If the number is 100,000 or more per ml, it is determined to be significant. If the number is 1,000,000 or more per ml, bacterial infection (bacteriuria) is definite. Therefore, usually 10,000 / ml, 100,000 / m
A standard color contrast diagram for three points of 1 million / ml may be created. In FIG. 2, 1000 / ml and 100
A color control chart is shown in which color numbers are classified into five levels by adding the ones of 100,000 / ml.

Further, the bacteria sample introduction part C has a funnel-shaped introduction hole 7 at the center, and a pre-filter 8 is attached to the introduction hole 7. This bacterial sample introduction part C
Usually, as shown in the figure, the entire shape is substantially hat-shaped, and a funnel-shaped introduction hole 7 is provided at the center thereof. Usually, a pre-filter 8 is mounted at the lower end of the funnel-shaped introduction hole 7. The pre-filter 8 is used to collect (pre-treat) such large animal cells in advance when animal cells larger than bacteria, such as leukocytes, coexist in the sample. Those having a pore size of about 6 to 8 μm are used. The overall size of the filter is not particularly limited, but the diameter (diameter) of the filter is preferably about 10 to 15 mm. As such a pre-filter 8, it is preferable to use a hydrophobic filter as described above because it has weak or no polarity and does not hinder the adsorption of bacteria.

In the first instrument for detecting bacteria of the present invention, a bacteria collecting / detecting section B is provided on the base A as described above, and a bacterial sample introducing section C is provided on the bacterial collecting / detecting section B. There is no particular limitation on the coupling means that can be sequentially coupled,
It can be performed by fitting or screwing. In FIG. 1, the base part A and the bacteria collecting / detecting part B are fitted, and the bacteria collecting / detecting part B and the bacteria sample introducing part C are screwed together. However, the present invention is not limited to this. In short, except for the connection port 2 with the suction means provided on the side of the base portion A, the airtightness is approximately closed (strictly speaking, since the outside air flows through the hydrophobic filter 5, the airtightness is completely closed). However, it is only necessary that they can be combined. By connecting the respective parts in this manner, the pre-filter 8 attached to the lower end of the funnel-shaped introduction hole 7 of the bacteria sample introduction part C and the tubular part penetrating vertically through the central part of the bacteria collection / detection part B The hydrophobic filter 5 and the hydrophobic filter 5 attached to the center of the upper end of the tubular portion 4 are arranged in a substantially straight line vertically. Bacterial collection / detection unit B
The hydrophobic filter 5 attached to the center of the upper end of the tubular portion 4 and the pre-filter 8 attached to the lower end of the funnel-shaped introduction hole 7 of the bacteria sample introduction section C are located at positions almost in contact with each other. To

The size of the first bacteria detection instrument of the present invention is not particularly limited. For example, when the shape of the bacteria sample introduction part C is substantially hat-shaped as shown in the figure, the diameter of the first bacteria detection instrument is approximately one. About 20 to 40 mm, the diameter of the upper end of the funnel-shaped introduction hole 7 is about 10 to 15 mm, the diameter of the bacteria collecting / detecting section B is about 20 to 25 mm, and the height when the whole is combined is 3
It is about 5 to 40 mm. The capacity of the bacterium detection device of the present invention may be selected according to the amount of a washing solution or the like to be used. Also, there is no particular limitation on the material, but in consideration of ease of production and cost, a resin made of polyethylene, polypropylene, polystyrene, or the like is usually used, and a glass material is used. .

The first bacteria detection device of the present invention has the above structure. The first bacteria detection instrument of the present invention is used for detecting the number of bacteria in combination with a suction means, a dye solution, and a washing solution. Hereinafter, the first of the present invention
A method for detecting bacteria using the bacteria detection instrument of the present invention will be described. In this case, all operations are performed under suction conditions.

For detection, first, the water absorbing material 3 is put in a low position inside the base part A, and then the mist adsorbing material 9 is put in the connection port 2 with the suction means provided on the side. A separately prepared suction means is connected to the port 2. The connection with the suction means may be performed after the following overall connection. Next, the bacteria collecting / detecting section B is connected to the upper portion of the base portion A, and the bacterial sample introducing section C is further connected to the upper portion of the bacterial collecting / detecting section B as described above. The whole is combined in an approximately sealed state to prepare for detection. In this way, by being connected so as to be in a substantially sealed state, suction becomes possible.

After the whole components have been connected as described above, a sample containing bacteria (eg, urine) is introduced into the fungal sample introduction part C in a funnel shape by using a sampling pipette or the like while aspirating with a suction means. The solution is dropped onto the pre-filter 8 through the hole 7. The amount of the sample (sample) is about 2 to 3 drops, and is usually 50 to 500 μl, preferably 100 to 500 μl.
300 μl.

The sample whose number of bacteria can be detected by the first bacteria detection device of the present invention is not particularly limited. For example, urine, water-soluble metal working oil such as cutting oil, rolling oil, heat treatment oil, etc. , Liquid seasonings (eg, soy sauce, sauces, etc.), liquid foods and drinks (eg, fermented milk, lactic acid bacteria drinks, etc.),
Liquid foods such as liquors (eg wine, sake), washed samples in the case of powders and solid foods (eg vegetables and fish meat), water-soluble paints, etc., as well as river water, pond water, aquarium water, domestic wastewater, etc. It can be widely applied to water and the like. The sample may be appropriately diluted as needed, or, for example, in the case of a solid food, may be crushed and then diluted to obtain a measurement sample. The bacterium detection device of the present invention includes bacteria present in such a sample, for example, Escherichia Coli, Staphylococcus aureus (
Staphylococcus aureus), Pseudomonas aerug
inosa), etc., to quickly measure the total number.

When a sample containing bacteria is dropped onto the prefilter 8 while sucking in this manner, animal cells larger than bacteria, such as leukocytes, contained in the sample containing bacteria remain on the prefilter 8. , Will be removed.

On the other hand, the sample containing bacteria, which has passed through the pre-filter 8, is then attached to the center of the upper end of the tubular portion 4 which penetrates vertically through the center of the bacteria collecting / detecting section B. 5 will be encountered (contacted). As a result, the bacteria in the sample are collected by the hydrophobic filter 5.
Here, the method of performing suction filtration as in the present invention can perform collection, staining, and washing by a series of operations while suctioning, compared to the pressure filtration method, and replace the filter as in the pressure filtration method. This is preferable because there is no operation such as the above. The remaining sample solution that has passed through the hydrophobic filter 5 passes through the tubular portion 4 vertically penetrating the center of the bacteria collecting / detecting section B, and passes through the water absorbing material placed under the tubular portion 4. Absorbed in 3.

Next, if necessary, a cleaning solution is dropped from above the pre-filter 8 for cleaning. The washing liquid may be the same as the washing liquid used for washing the dye described below.
About 2 to 3 drops of this washing liquid is sufficient, and usually 50 drops.
５００500 μl, preferably 100-300 μl.
This washing is also performed under suction conditions. This washing improves sensitivity and facilitates filtration after dyeing.

Next, the sample (bacteria) collected and washed by the hydrophobic filter 5 for collecting bacteria by suction filtration as described above is stained. Here, various dyes are used to stain bacteria, and these are used as dye solutions, and the dye solutions are added to the hydrophobic filter 5 for collecting bacteria after the bacteria are collected under suction conditions (usually dripping). It should be done by doing. The dye used for staining may be any dye that can be used for staining bacteria, and examples thereof include fuchsin, safranin, crystal violet, and Victoria Blue. In particular, fuchsin is preferred in terms of easy removal of excess dye. , Safranin are preferred.

The above dye is used as an aqueous solution (dye solution). When preparing a dye solution, a preservative, a surfactant and the like can be added as necessary. The concentration of the dye solution is usually 0.0005 to 2.0% (w / v), preferably 0.002 to 1%.
% (W / v). Here, the concentration of the dye solution is 0.0005%
If the amount is less than 10%, the coloring becomes insufficient. On the other hand, if the concentration of the dye solution exceeds 2.0%, it becomes difficult to remove the excess dye. If necessary, 1 to 20% (v / v) may be added when ethanol is added as a preservative to the above-mentioned dye solution, and when a surfactant is added, surfactant may be added. 0.0001-1% (w
/ V). In preparing the above-mentioned dye solution, a dye solution having a predetermined concentration may be prepared in advance, and the dye solution may be diluted with a washing solution as described later to have a desired concentration. In addition, the amount of dye solution added (the amount dropped)
Is enough for 2-3 drops, usually 100-300 μl
It is. Further, the ratio of the amount of the dye solution to the amount of the sample solution is 0.1.
2 to 3.0, preferably 0.5 to 2.0. 0.2
If it is less than 3.0, the coloring becomes poor, and if it exceeds 3.0, the dye liquid is wasted, and both are not preferred.

Thereafter, the bacteria sample introduction part C is removed, and
The prefilter 8 attached to the lower end of the funnel-shaped introduction hole 7 of the bacteria sample introduction part C is removed. If the prefilter 8 is attached to the bacterial sample introduction part C in advance and used, the prefilter 8 is automatically removed when the bacterial sample introduction part C is removed. After removing the pre-filter 8, washing may be performed, or dyeing may be performed by adding (dropping) a dye solution.

In this way, the excess dye is removed from the sample (bacteria) collected and stained by the hydrophobic filter 5 for collecting bacteria by washing. Specifically, the washing is performed by dropping the washing liquid on the hydrophobic filter 5 attached to the center of the upper end of the tubular part 4 of the bacteria collecting / detecting unit B under the suction condition. As the washing liquid used here, water and various buffer solutions (with a pH of about 6 to 8) can be used. Further, if necessary, those to which various surfactants are added may be used. The surfactant is
It may be added at a ratio of 0.0001 to 1.0% (w / v).

The amount of the washing solution used depends on the suction filtration area of the hydrophobic filter (animal cell collecting area, that is, the colored area), so it is difficult to determine the amount uniquely.
A few drops are sufficient, usually 50-500 μl, preferably 100-200 μl. In this case, if the used amount of the washing solution is less than 50 μl, the washing is insufficient, while if the used amount of the washing solution exceeds 500 μl, the dye may leak from the bacteria. The amount of the washing solution used is preferably the same as the amount used for preparing a color control table or a calibration curve, and the amount used for washing a sample with an unknown number of bacteria, from the viewpoint of suppressing errors.

As described above, according to the first bacterium detection device of the present invention, collection on the hydrophobic filter 5 for collecting bacteria,
Staining and washing off excess dye can all be performed under suction conditions. Therefore, all operations can be performed under suction filtration conditions, and continuity of operations can be maintained. As described above, using the first bacteria detection device of the present invention, bacteria are collected on a hydrophobic filter while being suction-filtered, and stained,
Next, the excess dye may be removed by washing, and the number of bacteria in the sample may be determined from the degree of bacterial coloring.

The number of bacteria in a sample is measured visually. That is, since the color contrast FIG. 6 is affixed around the hydrophobic filter 5 attached to the bacteria collecting / detecting portion B of the bacteria detecting instrument of the present invention, the coloring degree of the hydrophobic filter 5, The control figure of this color is visually compared to determine the number of bacteria in the sample. That is, in the first bacterium detection device of the present invention, since the color control diagram 6 of a color prepared in advance using a sample of a known amount of bacteria is attached, the hydrophobic filter 5 for collecting bacteria is used. The number of bacteria in the sample may be measured by comparing the degree of coloration of the bacteria present above, that is, the intensity of the color, with the color control figure 6.

Next, a description will be given of the second bacterium detection device of the present invention. The base portion A in the second bacteria detection device of the present invention basically has the opening 1 in the upper part and the side portion in the same manner as the base portion A in the first bacteria detection device of the present invention described above. It has a connection port 2 with the suction means and has a substantially cylindrical shape with a water absorbing material 3 placed inside. That is,
The connection port 2 and the water absorbing material 3 are the same as those in the first bacteria detection device of the present invention described above, and the mist adsorbing material 9
It is also preferable that the support 10 is provided. As shown in FIGS. 4 to 9, the connection port 2 for the suction means provided on the side portion is literally simply a connection port, and the connection component 15 containing the mist adsorbent 9 is connected to the connection port. Alternatively, a separately prepared suction pump may be connected to this end. However, a guide groove 13 is formed in the base portion A of the second bacterium detection device of the present invention, and a claw-shaped portion 14 provided in a bacterium sample introduction portion C described later is provided in the guide groove 13. It is designed to enter. Here guide groove 1
3 has a gentle hill-like shape (substantially “H” shape) as shown in the figure when viewed from the horizontal direction. For example, by rotating the base portion A clockwise, the “H” It is loosened at the location corresponding to the uppermost part of the letter, and is further rotated to be positioned downward, so that it gradually becomes tighter. In addition, a pictorial display indicating the rotation direction may be provided below the guide groove 13 as illustrated.

Next, the bacteria collecting / detecting portion B in the second bacteria detecting device of the present invention has a plurality of tubular portions 4 penetrating vertically, and covers at least the upper surface of the tubular portions 4. It is equipped with a hydrophobic filter 5 and is used for collecting and collecting bacteria in the first bacteria detection device of the present invention.
The detection unit B differs from the detection unit B in that a plurality of tubular portions 4 are provided, and also differs in that there is no color contrast diagram 6. In addition, in the second bacteria detection device of the present invention, the step portion 11 does not have to be particularly provided. Further, it is preferable that the lower end of the tubular portion 4 penetrating up and down reach near the bottom surface of the base portion A when fitted to the base portion A. This is the same as the bacteria collection / detection unit B in the detection instrument. The upper surface of the bacteria collecting / detecting section B is circular and flat, and a hydrophobic filter 5 is provided on this upper surface so as to cover at least the upper surface of the tubular portion 4 (in the figure, the entire upper surface). It is installed.

The hydrophobic filter 5 used in the second embodiment of the present invention is the same as that shown in the first embodiment of the present invention. In addition to the above, it is preferable because it is easy to remove, and the filtration pore size and suction filtration area (coloring area) of the hydrophobic filter 5 for collecting bacteria are the same as those shown in the first part of the present invention. It is.

However, a color contrast diagram is not affixed to the bacteria collecting / detecting section B and is prepared separately. The color contrast diagram itself may be the same as that shown in the first embodiment of the present invention (for example, the same as that shown in FIG. 2). Further, a plurality of tubular portions 4 are provided, and the illustrated one shows four provided in correspondence with the number of installations 4 of the funnel-shaped introduction holes 7 in the bacterial sample introduction portion C described later. However, the present invention is not limited to this.
Book, five, six, etc.
Considering the convenience of operation, it is usually preferable to use about four.

Further, in the bacteria sample introduction part C of the second bacteria detection device of the present invention, a plurality of funnel-shaped introduction holes 7 are provided on the upper surface in a circumferential shape, and the plurality of introduction holes 7 are formed. A second introduction hole 7 ′ is provided at an intermediate position on a circumference between the adjacent introduction holes 7, and a pre-filter 8 is attached to a lower end of the introduction hole 7,
This is considerably different from the bacteria sample introduction part C in the first bacteria detection instrument of the present invention described above.

That is, first, a plurality of bacteria sample introduction parts C in the second bacteria detection device of the present invention (4 in the figure)
(1) the point that the funnel-shaped introduction hole 7 is provided in a circumferential shape, and only one funnel-shaped introduction hole 7 is provided; C
Is different from However, this is the same in that the overall shape of the bacterial sample introduction part C is substantially hat-shaped. Usually, a pre-filter 8 is provided at the lower end of the funnel-shaped introduction hole 7.
Is installed. This point is the same as the case of the bacteria detection device of the first invention. Further, the plurality of introduction holes 7 are provided in the bacteria sample introduction portion C in the second bacteria detection device of the present invention.
The second introduction hole 7 'is provided at an intermediate position on the circumference between the adjacent introduction hole 7 and the second introduction hole 7 (four are provided in FIGS. 4 to 9). This is different from the bacteria sample introduction part C in the first bacteria detection device of the present invention, which is not provided. The introduction hole 7 and the second introduction hole 7 ′ are both provided at least two or more.
The rotation angle when the washing operation of the excess dye is performed later will be different. For example, as shown in FIGS.
When a total of eight introduction holes, namely, one introduction hole 7 and four second introduction holes 7 ′, are provided, the rotation angle at the time of performing the excess dye washing operation later is 分 の rotation (45 degrees). If, for example, three introduction holes 7 and three second introduction holes 7 ′ are provided, that is, a total of six introduction holes, the rotation angle at the time of performing the excess dye washing operation later is 6 minutes. One rotation (60 degrees). The material and size of the prefilter 8 are the same as those described in the bacteria sample introduction section C of the first bacteria detection device of the present invention. Further, the bacterial sample introduction part C may be provided with a pictorial display indicating a rotation direction, an angle, or the like, if necessary. Further, it is preferable to provide a dent or the like around the bacterial sample introduction part C in order to prevent slippage.

In the second bacterium detecting device of the present invention, the bacterium collecting / detecting section B is provided on the base section A as described above, and the bacterium sample introducing section is provided on the bacterium collecting / detecting section B. C are sequentially coupled, and the tubular portion 4 and the introduction hole 7 are arranged so as to be linear in the vertical direction. It is rotatable in the left-right direction. The connecting means is the same as that of the first bacteria detection device of the present invention described above. Since the tubular portion 4 and the introduction hole 7 are arranged in a positional relationship such that the bacterial sample introduction portion C is horizontally aligned by rotating the bacteria sample introduction portion C left and right, the respective portions are thus combined, By rotating the bacteria sample introduction part C to the left and right as appropriate, the tubular part 4 and the introduction hole 7 are arranged in a straight line in the vertical direction. In this state, the bacteria collection / detection unit B
The hydrophobic filter 5 mounted so as to cover the upper surface of the tubular portion 4 and the pre-filter 8 mounted at the lower end of the introduction hole 7 in the bacterial sample introduction portion C are located at positions substantially in contact with each other. become.

Further, in the second bacterium detection device of the present invention, the bacterium sample introduction part C is rotatable left and right with respect to the base part A. A guide groove 13 having a specific shape is formed in the base portion A of the instrument, and the claw-shaped portion 14 provided in the bacteria sample introduction portion C is inserted into the guide groove 13. By rotating the base A clockwise, the bacterial sample introduction part C slightly moves up and down with respect to the base A.

The material of the second germ detection device of the present invention is the same as that of the first germ detection device of the present invention, and the size thereof has a plurality of inlets 7 and the like. Slightly,
It is larger than the first bacteria detection device of the present invention.

The second bacterium detection device of the present invention has the above structure. The second bacteria detection device of the present invention is used for detecting the number of bacteria in combination with a suction means, a dye solution, a washing solution, and a color contrast diagram, and is characterized in that the color contrast diagram is separately used. This is different from the first bacteria detection device. Hereinafter, a method for detecting bacteria using the second bacteria detection device of the present invention will be described. In this case, all operations are performed under suction conditions, as in the first aspect of the present invention.

For detection, the water-absorbing material 3 is first placed in a low position inside the base A, and then the mist adsorbing material 9 is placed in the connection port 2 with the suction means provided on the side. The connection of a separately prepared suction means to the mouth 2 is the same as in the first bacteria detection device of the present invention. Next, a bacteria collecting / detecting part B is connected to the upper part of the base part A. Further, a bacterial sample introducing part C is provided above the bacterial collecting / detecting part B.
By rotating the tube part 4 and the introduction hole 7 in the vertical direction in a straight line, and by rotating the tube part to the right and left as appropriate, the whole part is joined so as to be in a substantially sealed state. Prepare for detection. In this way, by being connected so as to be in a substantially sealed state, suction becomes possible.

After the whole components have been combined as described above, a sample containing bacteria (eg, urine) is drawn into the funnel-shaped plural portions of the bacteria sample introduction part C by using a sampling pipette or the like while sucking by suction means. Through the pre-filter 8
To be dropped. The amount of the sample (sample) is about 2 to 3 drops, and is usually 50 to 500 μl, preferably 1 to 500 μl.
00 to 300 μl.

The sample whose number of bacteria can be measured by the second bacterium detecting device of the present invention is the same as that shown in the description of the first bacterium detecting device of the present invention.

When a sample containing bacteria is dropped onto the prefilter 8 while sucking in this manner, animal cells larger than bacteria, for example, leukocytes, contained in the sample containing bacteria remain on the prefilter 8. , Will be removed.

On the other hand, the bacteria-containing sample that has passed through the pre-filter 8 is then subjected to a hydrophobic filter mounted so as to cover the upper surface of the vertically extending tubular portion 4 in the bacteria collection / detection section B. 5 will be encountered (contacted). As a result, the bacteria in the sample are collected by the hydrophobic filter 5. The remaining sample solution that has passed through the hydrophobic filter 5 passes through the tubular part 4 of the bacteria collection / detection unit B that penetrates up and down, and enters the water absorbing material 3 placed below the tubular part 4. Absorbed.

Next, if necessary, a cleaning solution is dropped from above the pre-filter 8 for cleaning. Second of the present invention
The washing in the bacteria detection instrument of the present invention is the same as the washing in the first bacteria detection instrument of the present invention described above.

Next, the sample (bacteria) collected and washed by the hydrophobic filter 5 for collecting bacteria by suction filtration as described above is stained. Second of the present invention
The staining in the bacteria detection instrument of the present invention is the same as the staining in the first bacteria detection instrument of the present invention described above.

Thereafter, in the first bacteria detection device of the present invention, the bacteria sample introduction part C is removed, and the pre-filter 8 attached to the lower end of the funnel-shaped introduction hole 7 of the bacteria sample introduction part C is removed. However, in the second bacteria detection device of the present invention, it is not necessary to remove the bacteria sample introduction part C, and the bacteria sample introduction part C is rotated, for example, clockwise by a predetermined angle (8 in FIGS. 4 to 9). The same effect as when the bacterial sample introduction part C is removed can be achieved by performing the rotation by one-half turn). That is, for example, by rotating the bacterial sample introduction part C one-eighth clockwise, the second introduction hole 7 ′ adjacent to the specific funnel-shaped introduction hole 7 is rotated by the conventional funnel-shaped introduction hole. Since the second introduction hole 7 ′ is located at the position of the hole 7, the second introduction hole 7 ′ and the tubular portion 4 are linearly arranged in the vertical direction, and the pre-filter 8 attached to the lower end of the funnel-like introduction hole 7 is a funnel-shaped. It rotates together with the introduction hole 7, resulting in the same result as the removal of the pre-filter 8.

Therefore, in this state, the excess dye can be removed by dropping the cleaning liquid from the second introduction hole 7 '. In this way, excess dye is removed from the sample (bacteria) collected and stained by the hydrophobic filter 5 for collecting bacteria by washing. Specifically, when the washing liquid is dropped from the second introduction hole 7 ′ provided in the bacteria sample introduction part C under the suction condition, the washing liquid passes through the second introduction hole 7 ′ and passes through the bacteria collection / detection part. B is dropped on the hydrophobic filter 5 mounted on the upper surface of the tubular portion 4 of B, and as a result, cleaning is performed. The cleaning liquid used here and the amount used are as described in the first part of the present invention.

As described above, according to the second bacterium detecting device of the present invention, collection by the hydrophobic filter 5 for collecting germs,
Not only can staining and removal of excess dye be carried out under suction conditions, but it is not necessary to remove the bacterial sample introduction part C to remove the pre-filter, thereby removing biohazard. Inlet hole 7
Thus, a plurality of specimens (bacterial samples) can be measured simultaneously. As described above, using the second bacteria detection device of the present invention, a plurality of specimens (bacteria samples) are collected on each hydrophobic filter while being suction-filtered, stained, and then excess dye is washed. The bacteria may be removed and the number of bacteria in each sample may be measured based on the degree of coloring of each bacteria.

In the present invention, a color contrast chart is prepared separately, and the coloring degree of each hydrophobic filter 5 obtained as described above is compared with the color contrast chart prepared separately. Are visually compared to determine the number of bacteria in each sample. As described above, it is sufficient to prepare the same color contrast diagram as that shown in the first embodiment of the present invention.

[0058]

Next, the present invention will be described in more detail with reference to embodiments. Example 1 Using a bacteria detection instrument as shown in FIG. 1, urine was sampled from urine released from a bacteriuria patient (female, 38 years old), a healthy person (male, 54 years old) and a healthy person (female, 23 years old). The number of bacteria in the was measured. Here, the size of the bacteria detection instrument is such that the diameter of the bacteria sample introduction part C is 30 mm, the diameter of the upper end of the funnel-shaped introduction hole 7 is 12 mm, and the diameter of the bacteria collection / detection part B is 23 mm.
mm and the height when the whole was combined was 37 mm.
As the prefilter 8, a polypropylene prefilter 8 (diameter: 7 μm) having a diameter of 13 mm was used to remove large cells other than bacteria. Furthermore, the hydrophobic filter 5 for collecting bacteria has a diameter of 1 mm.
A 3 mm polytetrafluoroethylene membrane filter (filtration pore diameter 3 μm, manufactured by Toyo Roshi Kaisha) was used. First, a water-absorbing material (filter paper) 3 is put in a low position inside the base portion A, and then a mist adsorbing material (cotton) 9 is put in a connection port 2 with a suction means provided on a side portion. A suction pump prepared separately was connected to the port 2.

Next, on the upper part of the base part A, bacteria
The detection section B was connected, and further, the bacterial sample introduction section C was connected to the upper portion of the bacteria collection / detection section B, and the whole was connected so as to be in a substantially sealed state, thus preparing for detection. . In addition, the diameter of the portion to be suction-filtered is 3 mm,
The upper part of the bacteria collection / detection unit B is configured. After the whole components are combined as described above, two drops (about 100 μl) of the bacteria-containing sample are drawn into the funnel-shaped introduction hole 7 of the bacteria sample introduction part C by using a sampling pipette or the like while sucking the sample with a suction pump. From the pre-filter 8
Animal cells larger than the bacteria contained in the sample containing the bacteria were removed by the pre-filter 8, and the bacteria were collected on the hydrophobic filter 5 for collecting bacteria. Next, a washing solution [0.05% (W / V) Tween 20 added PBS buffer, p.
[H7.0] was dropped and washed. Then, fuchsin stain (concentration 0.005%)
, W / V) was dropped, and the standard sample collected on the hydrophobic filter 5 for collecting bacteria was stained.

Next, the bacteria sample introduction part C is removed,
The prefilter 8 attached to the lower end of the funnel-shaped introduction hole 7 of the bacteria sample introduction part C was removed. Next, the washing liquid [0.05% (W / V) Tween 20 added PBS] was added under suction conditions.
Buffer, pH 7.0] 100 µl of bacteria collection / detection section B
The excess dye was removed by dripping onto a hydrophobic filter 5 attached to the center of the upper end of the tubular portion 4 of the above. All operations were performed under suction conditions. Before use, the reagents used were filtered through a 0.5 μm polytetrafluoroethylene (PTFE) filter (manufactured by Toyo Roshi Kaisha) to remove fine particles. The degree of coloration of the bacteria existing on the hydrophobic filter 5 for collecting bacteria (the degree of coloration of a circle having a diameter of 3 mm) is visually observed and compared with the color control figure 6 attached around the hydrophobic filter 5. The results are shown in Table 1 below. Similar results were obtained when sterile water was used as a diluent instead of urine. The measurement time was 30 seconds. For comparison,
The number of bacteria measured on the CLED agar plate medium is also shown.

[0061]

[Table 1] Table 1

The color control shown in FIG. 6 was prepared as follows. Escherichia coli (Escherich
ia coli) (ATCC 11303) and Staphylococcus aureus (Staph
ylococcus aureus) (IFO 3183), the following tests were performed. The broth broth medium is inoculated with the inoculum and incubated at 37 ° C.
For 24 hours, and the culture solution was used as a standard sample bacterial suspension. On the other hand, collecting urine released from healthy males,
Filtration was performed using a polytetrafluoroethylene membrane filter (manufactured by Toyo Filter Paper) having a filtration pore size of 0.5 μm to prepare a bacteria diluting solution. A predetermined amount of the bacterial dilution was added to the standard sample bacterial suspension, and the number of bacteria was 1000
Pcs / ml, 10,000 / ml, 100,000 / ml
About 1 million pieces / ml, about 10 million pieces / ml
Five standard samples were prepared so as to have a concentration of about the same.
The number of bacteria was determined on a CLED agar plate medium at 37 ° C and 24 ° C.
Counting was performed based on the number of colonies after culturing for an hour.

Thereafter, the bacteria were collected and stained in the same manner as in the measurement of the number of bacteria as described above. The degree of coloring of bacteria existing on the hydrophobic filter 5 for collecting bacteria (
The degree of coloring of a circle having a diameter of 3 mm) was visually observed, and the results are shown in Table 2 below. Similar results were obtained when sterile water was used as a diluent instead of urine. These colored filters were taken as a standard sample in a color photograph to provide a color control chart. In addition, there was no significant difference in the degree of coloring between Escherichia coli and Staphylococcus aureus.

[0064]

[Table 2] Table 2

Example 2 Escherichia Coli (ATCC 11303) was inoculated into a broth broth liquid medium, and allowed to stand at 37 ° C. for 24 hours to obtain a culture solution. On the other hand, Staphylococcus aureus (Sta
phylococcus aureus) (IFO 3183) was inoculated into a broth broth liquid medium, and allowed to stand at 37 ° C for 24 hours.
A culture solution was obtained. For each of the obtained cultures, 1
A 100-fold diluted solution and a 1000-fold diluted solution were prepared, and the same operation as in Example 1 was performed on these diluted solutions to measure the number of bacteria. The measurement time was 30 seconds. The results are shown in Table 3. For comparison, the number of bacteria measured on the broth bouillon agar plate medium is also shown. The color comparison table used in Example 1 was used.

[0066]

[Table 3] Table 3

Comparative Example 1 According to the method described in JP-A-4-218392,
When the number of bacteria in urine was measured, the measurement time was 3 minutes.

Example 3 Urine released from a bacteriuria patient (female, 50 years old) and a healthy person (male, 55 years old), two kinds of lactic acid bacterium drinks using a bacteria detection instrument as shown in FIGS. Trade name: York, manufactured by Nisshin Yoke, trade name: Yakult, manufactured by Chiba Yakult Kogyo). The urine sample was used as is, and the lactic acid bacteria drink was
The analysis was performed on the liquid diluted 1000 times. Here, the bacteria detection instrument has four funnel-shaped inlet holes 7, the size of which is 42 mm in the diameter of the bacteria sample inlet C, the diameter of the upper end of each funnel-shaped inlet hole 8 mm, and the size of the bacteria detection device. Collection / detection unit B
Has a diameter of 30 mm and a total height of 35 m when combined
m. As the prefilter 8, a polypropylene prefilter 8 (diameter: 7 μm) having a diameter of 13 mm was used to remove large cells other than bacteria. Furthermore, as the hydrophobic filter 5 for collecting bacteria, a 25 mm diameter polytetrafluoroethylene membrane filter (filtration pore diameter 3 μm, manufactured by Toyo Filter Paper) was used. First, a water absorbing material (absorbent cotton) 3 is put into the inside of the base part A, and then a connecting part 15 containing a mist adsorbing material (cotton) 9 is connected to a connection port 2 for suction means provided on the side. ,
Furthermore, a suction pump prepared separately was connected to this end.

Next, on the upper part of the base part A, bacteria
The detection section B was connected, and further, a bacterial sample introduction section C was connected to the upper portion of the bacteria collection / detection section B. At the time of coupling, the four claw-shaped parts 14 of the bacterial sample introduction part C are inserted into the center part (uppermost part of the curved groove) of the guide groove 13 provided in the base part A and coupled, and rotated clockwise. Then, the whole was joined so as to be in a substantially sealed state, and was prepared for detection.
The upper part of the bacteria collecting / detecting part B is configured so that the diameter of the part to be suction-filtered is 3 mm, and the lower part (3 mm in diameter) of each funnel-shaped introduction hole 7 in the bacteria sample introduction part C. Is configured. A second introduction hole 7 ′ (diameter 10 mm) is provided in the bacteria sample introduction portion C at 4 mm.
Although it is provided, it is used for washing excess dye as described later. After the stain is added, when the bacterial sample introduction part C is rotated clockwise by one eighth, the colored part of the hydrophobic filter 5 for collecting bacteria is positioned at the center of the second introduction hole 7 '. The part is configured to come. The pre-filter 8 is attached to the bacteria sample introduction part C, and the hydrophobic filter 5 for collecting bacteria is attached to the bacteria collection / detection part B, respectively.

After the whole components have been combined as described above, two drops (about 100 μl) of the bacteria-containing sample are respectively applied to the funnel-shaped funnel of the bacteria sample introduction part C by using a sampling pipette while sucking with a suction pump. By dropping the pre-filter 8 from the introduction hole 7 onto the pre-filter 8, animal cells larger than the bacteria contained in the sample containing bacteria are removed by the pre-filter 8, and the hydrophobic filter 5 for collecting bacteria is removed.
The bacteria were collected. Since there are four funnel-shaped introduction holes 7, four analysis can be performed. Next, the washing liquid [0.05% (W / V)
) Tween 20 added PBS buffer, pH 7.0]
Washing was performed by dropping (about 100 μl) dropwise. afterwards,
Fuchsin staining solution (concentration 0.005%, W
/ V) was dropped, and the bacteria collected by the hydrophobic filter 5 for collecting bacteria were stained.

Next, the bacterial sample introduction part C is rotated by one-eighth clockwise to move the pre-filter 8 to remove it, and then the washing solution [0.05% (W / V) Tween] is similarly aspirated. 20 μl PBS buffer, pH 7.0] 100 μl
Was dropped onto the hydrophobic filter 5 for collecting bacteria from above the second introduction hole 7 ′ to perform washing to remove excess dye. All operations were performed under suction conditions. Before use, the reagents used were filtered through a 0.5 μm polytetrafluoroethylene (PTFE) filter (manufactured by Toyo Roshi Kaisha) to remove fine particles. The degree of coloration of the bacteria (the degree of coloration of a circle having a diameter of 3 mm) present on the hydrophobic filter 5 for collecting bacteria was visually observed by comparison with the color control chart of Example 1 and Table 4 below was obtained. It was as follows. For comparison, the number of bacteria measured by agar plate culture is also shown. For urine samples, CLED agar plate medium (manufactured by Nissui Pharmaceutical Co., Ltd.) was used.
Each was cultured using a P-added plate count agar medium (manufactured by Eiken Chemical Co., Ltd.), and the number of bacteria was counted from the number of colonies.

[0072]

[Table 4] Table 4

[0073]

According to the (first and second) bacteria detection devices of the present invention, bacteria in a sample can be collected on a hydrophobic filter while being suction-filtered, and then stained and washed. A series of operations for collecting, staining, and washing bacteria can be performed continuously under suction filtration conditions, and the operation steps are simple.
Therefore, according to the bacterium detection instrument of the present invention, the number of bacteria in a sample can be measured quickly and easily without requiring any special technique or knowledge. According to the bacterium detection device of the present invention, the determination can usually be made within 30 seconds. Moreover, according to the bacterium detection device of the present invention, the presence or absence of bacteria can be determined without culturing the bacterium, so that it can be used for specimen screening for bacterial tests in hospitals, and can contribute to reducing costs for patients and hospitals. . In addition, the bacteria detection device of the present invention is very simple, and only a suction means such as a suction pump, a dye solution, a washing solution, etc. need be prepared, and no special device is required. It can also be measured at clinics. When there is no suction pump or suction equipment, suction can be performed using a syringe, and measurement can be performed even in a place without a power supply. Therefore, measurement can be performed in a room without a power supply or outdoors. Furthermore, the bacteria detection device of the present invention can be applied to many bacteria by selecting the filtration pore size of the hydrophobic filter, so that the application range is extremely wide. Further, the bacteria detection device of the present invention is extremely simple and inexpensive, and does not require any special device, and thus has an advantage that it can be used in any field. In particular, since the bacteria detection device of the present invention can be used by ordinary laymen, it is possible to easily inspect the quality of water around us, such as rivers, seas, and ponds, and contribute to environmental conservation. There is. Furthermore, according to the second bacteria detection device of the present invention, it is not necessary to remove the bacteria sample introduction part C to remove the pre-filter, and the biohazard can be removed. Further, according to the second bacteria detection device of the present invention, a plurality of specimens (bacteria samples) can be measured simultaneously. Therefore,
INDUSTRIAL APPLICABILITY The present invention can be widely used for various tests including the urine test field (diagnosis area).

[Brief description of the drawings]

FIG. 1 is a central longitudinal sectional view showing one embodiment of the first bacteria detection device of the present invention, wherein FIG. 1 (a) shows a state of fitting and integration, and FIG. ) Indicates a disassembled state.

FIG. 2 is a bacterium collecting / detecting unit B in FIG.
FIG.

FIG. 3 is a central longitudinal sectional view showing another embodiment of the first bacteria detection device of the present invention.

FIG. 4 is a perspective view showing one embodiment of the second bacteria detection device of the present invention.

FIG. 5 is a side view showing one embodiment of the second bacteria detection device of the present invention.

FIG. 6 is a plan view showing one embodiment of the second bacteria detection device of the present invention.

FIG. 7 is a sectional view taken along the line II of FIG. 6;

FIG. 8 is a cross-sectional view of FIG.
Is a sectional view taken along the line II when the is rotated clockwise by 1/8.

FIG. 9 is a sectional view taken along the line II-II of FIG. 6;

[Explanation of symbols]

 Reference Signs List A base part B bacteria collection / detection part C bacteria sample introduction part 1 opening 2 connection port 3 water absorbing material 4 tubular part 5 hydrophobic filter 6 color contrast diagram 7 funnel-shaped introduction hole 7 'second introduction hole 8 pre Filter 9 Mist adsorbent 10 Abutment 11 Step 12 Hydrophobic filter support member 13 Guide groove 14 Claw portion 15 Connection part

──────────────────────────────────────────────────続 き Continued on front page (58) Field surveyed (Int. Cl. ⁷ , DB name) C12M 1/26-1/34

Claims (2)

(57) [Scope of request for utility model registration] 1. A substantially cylindrical base part A having an opening 1 at an upper part, a connection part 2 at a side part with a suction means and a water absorbing material 3 inside, and a vertical part at a central part. Bacterium having a tubular portion 4 penetrating through, a hydrophobic filter 5 is mounted on the center of the upper surface of the tubular portion 4, and a color contrast figure 6 is attached around the hydrophobic filter 5. Collection
A detection part B, and a bacteria sample introduction part C having a funnel-shaped introduction hole 7 at the center and a pre-filter 8 mounted on the introduction hole 7, wherein the bacteria are placed on the base part A. A bacterium detection instrument, wherein the collection / detection unit B is configured so that the bacteria sample introduction unit C can be sequentially coupled on the bacterium collection / detection unit B. 2. A substantially cylindrical base portion A having an opening 1 at an upper portion and a connection port 2 with a suction means at a side portion and having a water absorbing material 3 placed therein, and penetrating vertically. A bacteria collection / detection unit B having a plurality of tubular parts 4 and having a hydrophobic filter 5 attached so as to cover at least the upper surface of the tubular part 4, and a plurality of funnel-shaped introduction holes 7 on the upper surface are provided. The second introduction hole 7 ′ is provided in a circumferential shape, and is located at an intermediate position on the circumference between the plurality of introduction holes 7 and an adjacent introduction hole 7.
And a bacteria sample introduction part C in which a pre-filter 8 is attached to the introduction hole 7. The bacteria collection / detection part B is provided on the base part A by the bacteria collection part B. The bacterial sample introduction part C is arranged so as to be able to be sequentially coupled on the detection part B, and the tubular part 4 and the introduction hole 7 are arranged so as to be linear in the vertical direction, and the bacteria A bacterium detection instrument, wherein a sample introduction part C is rotatable in the left-right direction with respect to the base part A.