JP2017001749A - Sealing device for bag for cryopreservation - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a sealing device for bag for cryopreservation which can automatically seal an entrance port of the bag for cryopreservation and allows anyone to perform sealing processing safely and properly.SOLUTION: A sealing device for bag for cryopreservation comprises: a bag clamp device 56; a laser device 57; and a scanning structure 58 for movement operating, for example, the back clamp device 56. The bag clamp device 56 comprises a stationary holding block 67, a movable holding block 69, and a clamp actuator 70. The laser device 57 comprises a laser oscillator 104 and a condenser lens 107. The stationary holding block 67 comprises: a block base 73; a radiator 74; and a radiation holder 75. In a state that a sealing part 55 of the bag is held and fixed by the radiator 74 and the movable holding block 69, for example, the bag clamp device 56 is movement operated by the scanning structure 58, and an infrared radiation laser beam is radiated to the sealing part 55, for forming a seal bead 125 for sealing.SELECTED DRAWING: Figure 1

Description

  The present invention relates to a sealing device that seals and seals an opening portion of a cryopreservation bag used when cryopreserving a living tissue such as a human being, an animal, or a plant. The cryopreservation bag is formed as a bag-like container by welding two laminated fluororesin sheets with an infrared laser beam.

  This type of cryopreservation bag is disclosed in Patent Document 1, for example. In this case, two laminated thermoplastic resin films are sandwiched between a support and an infrared transmitting solid heat dissipating material (hereinafter simply referred to as a heat dissipating material) and pressed, and an infrared laser beam is applied to both films from the heat dissipating material side. To form a weld bead to form a bag-like cryopreservation bag.

  Regarding the sealing device of the present invention, Patent Document 2 discloses that the opening of a cryopreservation container is sealed by thermal welding. There, an opening and an opening are integrally provided in a plastic container main body that contains a biological sample. After the biological sample is accommodated in the container main body, the opening is thermally sealed with a sealer and sealed. Yes.

International Publication No. 2003-039843 (page 11, Example 1, FIG. 3) Japanese Patent Laying-Open No. 2007-302567 (paragraph number 0035, FIG. 1)

  Since the cryopreservation bag of Patent Document 2 is made of, for example, polyethylene terephthalate, even an impulse-type commercially available sealer can be sufficiently sealed. However, since the cryopreservation bag made of fluororesin needs to be sealed at a higher temperature, it is difficult to thermally weld the entrance and exit with a commercially available sealer to exhibit a high degree of sealing function without variation. . In a cryopreservation bag that cannot exhibit a high degree of sealing function, there is a risk that liquid nitrogen may enter the bag, and if the cryopreservation bag is taken out of the liquid nitrogen container without knowing that, the liquid nitrogen suddenly increases. If the bag is frozen, the cryopreservation bag may be damaged and the contents may be scattered. Cryopreservation bags are often used in clinical settings such as hospitals, and the main users, doctors and medical technicians, can safely and accurately seal the cryopreservation bags, and have advanced sealing. It is desired to be able to demonstrate the stopping function.

  It is an object of the present invention to provide a cryopreservation bag sealing device that can perform sealing processing of a cryopreservation bag simply and automatically, and can perform the sealing processing safely and accurately.

The sealing device for a cryopreservation bag according to the present invention irradiates an infrared laser beam toward a bag clamp device 56 that sandwiches and fixes the sealing portion 55 of the cryopreservation bag, and a sealing portion 55 that is sandwiched and fixed by the bag clamp device 56. a laser device 57 which includes a frame 59 for supporting the respective members 56, 5 7 above follow. Bag clamp device 56 includes a fixed clamping block 67 which is supported lifting the frame 59, the movable clamping block 69 which is separable rotatably supported with respect to the fixed clamping blocks 67 in the guide shaft 68 provided in the fixed clamping blocks 67, And a clamp actuator 70 for moving the movable clamping block 69 to and from the fixed clamping block 67. The laser device 57 includes a laser oscillator 104 and a condensing lens 107 that condenses the infrared laser beam emitted from the laser oscillator 104 toward the sealing portion 55 of the cryopreservation bag. The fixed clamping block 67 is mounted on the block base 73 having an infrared laser beam irradiation window 72 opened, a heat radiator 74 made of a solid material rich in infrared transmission and heat conduction, and the block base 73. And a radiator holder 75 for supporting the radiator 74 in a state of facing the irradiation window 72. In a state where the sealing portion 55 of the cryopreservation bag were sandwiched and fixed between the fixed clamping block 67 and the movable clamping block 69, by irradiating the infrared laser beam to the sealing portion 55, the sealing bead 125 of the state across the doorway 3 The sealing portion 55 is formed.

  The movable clamping block 69 includes a pair of left and right sliders 79 supported by a guide shaft 68 so as to be slidable back and forth, a plate-like movable base 80 fixedly supported by both sliders 79, and left and right of the front surface of the movable base 80. It is supported by a pair of fixed left and right block support shafts 81. The inner base 84 fixed to the guide shaft 68 is provided with a clamp actuator 70 for operating the movable clamping block 69 back and forth via the movable base 80 and the block support shaft 81. With the sealing portion 55 of the cryopreservation bag placed on the outer surface of the heat radiating body 74 of the fixed holding block 67, the movable holding block 69 is operated by the clamp actuator 70, and the fixed and movable holding blocks 67 and 69 are used for sealing. The part 55 is clamped and fixed.

  The movable clamping block 69 is detachably mounted on the block support shaft 81 via the first mounting tool 83. The radiator holder 75 is detachably attached to the block base 73 via the second attachment tool 77. The movable clamping block 69 and the radiator holder 75 are removed from the block support shaft 81 and the block base 73 and sterilized.

A focus adjustment structure for adjusting the focal position of the condenser lens 107 is provided inside the frame 59. The focus adjustment structure includes a lens base 108 supported by an inner frame 92 fixed to a frame 59 , a lens guide 109 fixed to the lens base 108, a front and rear slider 110 supported slidably forward and backward by the lens guide 109, and a front and rear A lens holder 111 that is fixed to the slider 110 and supports the condenser lens 107, and a focus adjustment actuator 112 that operates the lens holder 111 back and forth are provided. The focal position of the condensing lens 107 is adjusted according to the thickness of the sealing portion 55 held and fixed by the bag clamp device 56.

  The outer surface of the frame 59 is covered with a protective wall 117 that prevents exposure by an infrared laser leaking from the laser device 57. A protective cover 118 that prevents exposure by an infrared laser is provided on the outer surface of the bag clamp device 56 so as to be openable and closable.

With a sealing device cryopreservation bag according to the present invention, a bag clamp apparatus 56, to constitute a sealing device etc. laser device 5 7. The bag clamping device 56 includes a fixed clamping block 67, a movable clamping block 69, and a clamp actuator 70 for operating the movable clamping block 69. The bag 70 for cryopreservation is operated by operating the actuator 70. The sealing portion 55 can be clamped and fixed by the both clamping blocks 67 and 69. Furthermore, the block base 73, constitute a radiator body 74 and the heat radiating body holder 75 fixed clamping blocks 67 or the like, the infrared laser beam is irradiated to the sealing portion 55 through the heat radiator 74, the sealing bead 125 I was able to form.

According to the sealing device, and the moving operation of the movable clamping block 69 with a clamp actuator 70, after clamping the sealing portion 55, by operating the record Za device 57, a fluorine-based resin S1 · S2 and materials The entrance / exit 3 of the cryopreservation bag can be easily and automatically sealed. Although the sealing operation of the sealing portion 55 of the cryopreservation bag is performed by a doctor or a medical technician, since a series of welding operations are automatically performed after the sealing portion 55 is clamped by the bag clamp device 56, The sealing process of the cryopreservation bag can be performed safely and accurately. In addition, since the sealing process is always performed automatically under certain conditions, there is no variation in the welding results, and therefore, anyone can easily perform a sealing process that can exhibit a high level of sealing function in a stable state for cryopreservation. A bag sealing device can be provided. In particular, even a cryopreservation bag formed of a fluororesin that needs to be sealed at a high temperature can be properly sealed.

  When the movable sandwiching block 69 is supported by the pair of sliders 79, the movable base 80, and the pair of block support shafts 81 fixed to the movable base 80 so as to be slidable back and forth with respect to the guide shaft 68 of the fixed sandwiching block 67, the movable sandwiching is performed. The block 69 can be smoothly moved back and forth with respect to the fixed clamping block 67, and the parallelism of the clamping surfaces of the both clamping blocks 67 and 69 can be enhanced. Further, when the clamp actuator 70 is provided on the inner base 84 fixed to the guide shaft 68, the movable clamping block 69 is operated back and forth with the clamp actuator 70 having a simpler structure, and sealing is performed with both the fixed and movable clamping blocks 67 and 69. The portion 55 can be securely clamped.

  The movable clamping block 69 was detachably mounted on the block support shaft 81 via the first mounting tool 83. The radiator holder 75 is detachably attached to the block base 73 via the second attachment tool 77. According to such a sealing device, the movable clamping block 69 and the radiator holder 75 are removed from the block support shaft 81 and the block base 73 as necessary, and the movable clamping block 69 and the heat radiation to which the living tissue may adhere. The body holder 75 can be sterilized. Therefore, the hygienic management of the movable holding block 69, the radiator 74, and the radiator holder 75 can be facilitated, and the mouth treatment of the sealing portion 55 of the cryopreservation bag can be performed in a sanitary and safe state.

  When a focus adjustment structure that adjusts the focal position of the condenser lens 107 is provided, the focal position of the condenser lens 107 can be easily adjusted simply by operating the focus adjustment actuator 112. Therefore, the focal position of the condensing lens 107 is adjusted according to the difference in material and thickness of the fluororesin sheets S1 and S2 forming the cryopreservation bag, and the sealing portion 55 is welded under optimum welding conditions. can do.

  When the outer surface of the frame 59 is covered with a protective wall 117 and a protective cover 118 that can be opened and closed is provided on the outer surface of the bag clamp device 56, the infrared laser that leaks from the laser device 57 to the user when the sealing portion 55 is welded is used. Since it can prevent exposure and exposure, the sealing process of the sealing part 55 can be performed further safely.

It is a vertical side view of the principal part of the sealing device of the cryopreservation bag according to the present invention. It is a side view which shows schematic structure of the sealing apparatus which concerns on this invention. It is a front view of the sealing apparatus which concerns on this invention. It is the sectional view on the AA line in FIG. It is a disassembled perspective view of a bag clamp apparatus. It is a vertical side view which shows the attachment point of the sealing part with respect to a bag clamp apparatus. It is a vertical side view which shows the state by which the sealing part was clamped. It is a front view which shows the attachment point of the sealing part with respect to a bag clamp apparatus. It is a front view which shows the example of formation of a seal bead. It is a vertical side view of the manufacturing apparatus of the cryopreservation bag according to the present invention. It is a schematic front view of the manufacturing apparatus of the cryopreservation bag. It is a top view of the manufacturing apparatus of the bag for cryopreservation. It is a side view which shows the movement of an operation structure. It is sectional drawing which shows the formation condition of a welding bead. It is a front view which shows the modification of the bag for cryopreservation. It is a front view which shows the sealing structure of the bag for cryopreservation which concerns on FIG.

  1 to 14 show an embodiment of a sealing device for a cryopreservation bag and related devices according to the present invention. In the present invention, “front / rear”, “left / right”, and “up / down” follow the cross arrows shown in FIGS. In the sealing device according to the present invention, for example, the cryopreservation bag having the structure shown in FIG. 8 is sealed. Before describing the sealing device, the structure of the cryopreservation bag and the manufacturing apparatus thereof will be briefly described. Keep it.

  In FIG. 8, the cryopreservation bag irradiates two layers of fluororesin sheets S1 and S2 with an infrared laser beam to form a one-stroke outline outer bead 1 at the interface between the sheets S1 and S2. In addition, the storage unit 2 that stores the living tissue and the entrance / exit 3 that is continuous to the storage unit 2 are formed between the sheets S1 and S2 surrounded by the outer bead 1. The outer bead 1 that divides the housing portion 2 includes a pair of upper and lower parallel bead portions 4 and 5, a pair of left and right parallel bead portions 6 and 7, and bead portions 4 to 7. The four corner bead portions 8 are formed at the adjacent corner portions of the first and second corner bead portions 8, so that the accommodating portion 2 is formed in a vertically long rectangular shape with four corners dropped. The entrance / exit 3 is formed at the left and right center of the upper bead portion 4. By providing the corner bead portion 8, it is possible to eliminate the formation of a right-angle inner corner in the housing portion 2, and to take out the living tissue housed in the housing portion 2 without any excess.

  The fluorine-based resin sheets S1 and S2 are formed as a transparent sheet that allows transmission of an infrared laser using any one of a fully fluorinated resin, a partially fluorinated resin, and a fluorinated resin copolymer as a forming material. is there. Specific forming materials include polytetrafluoroethylene (PTFE), polychlorotrifluoroethylene (PCTFE), polyvinylidene fluoride (PVDF), polyvinyl fluoride (PVF), perfluoroalkoxy fluorine-based resin (PFA), four fluorine. Ethylene / hexafluoropropylene copolymer (FEP), ethylene / tetrafluoroethylene copolymer (ETFE), chlorotrifluoroethylene copolymer (ECTFE), and the like. In this embodiment, the fluororesin sheets S1 and S2 are formed of a sheet made of a tetrafluoroethylene / hexafluoropropylene copolymer having a thickness of 100 μm, and both sheets S1 and S2 are irradiated with an infrared laser beam. Thus, a cryopreservation bag was formed.

  In FIG. 11, the cryopreservation bag manufacturing apparatus irradiates an infrared laser beam toward the sheet fixing structure 15 on the base 14 and the two-layered fluororesin sheets S 1 and S 2 supported by the sheet fixing structure 15. And a scanning structure 17 for moving the sheet fixing structure 15 with respect to the infrared laser beam.

  The sheet fixing structure 15 includes a sheet table 18 that supports the two fluorine resin sheets S1 and S2, and a sheet pressing body 19 that holds and holds the fluorine resin sheets S1 and S2 placed on the sheet table 18. Configure. The sheet table 18 includes a table body 20 made of aluminum having high thermal conductivity and a table base 21 that fixes and supports the table body 20, and hinges 29 described later are mounted on the left and right of the rear end of the table base 21. A bracket 22 for projecting is projected upward (see FIG. 10). The upper surface (mounting surface) of the table main body 20 on which the fluorine resin sheets S1 and S2 are mounted is finished as a flush horizontal surface.

  The sheet pressing body 19 includes a heat radiating body 25 made of a solid material rich in infrared transmission and heat conduction, and a steel-made pressing frame 26 that supports the heat radiating body 25. The radiator 25 was formed of a single crystal silicon disk that was transparent to the infrared laser. As shown in FIG. 12, the pressing frame 26 is made of an octagonal metal frame, a circular laser window 27 is opened at the center thereof, and a circular mounting seat 28 is formed on the lower surface side of the window 27. The radiator 25 is fitted and fixed to the mounting seat 28.

  By connecting the rear portion of the pressing frame 26 and the bracket 22 of the table base 21 with a pair of left and right hinges 29, the entire sheet pressing body 19 is supported by the sheet table 18 so as to be swingable up and down. The sheet pressing body 19 can be displaced between the welding posture shown in FIGS. 12 and 13 and the standby posture shown in FIG. 10 by grasping the handle 30 and opening / closing it. The rubber block 31 provided at the center of the rear part of the sheet pressing body 19 is received by a stopper 32 fixed to the bracket 22 so that the sheet pressing body 19 that has been opened to the standby position can be held in a rearward tilted position. I have to. In this state, the two-layered fluororesin sheets S 1 and S 2 can be placed on the table body 20, or the blank body of the cryopreservation bag after the welding process can be taken out from the table body 20.

  The laser device 16 is a commercially available carbon dioxide laser unit. An irradiation tube 34 is projected from the side end of a rectangular box-like case that is long on the left and right sides, and laser light output from a resonator inside the case is changed. After turning downward by the direction mirror 35, the light is squeezed by the condenser lens 36 provided inside the laser head and irradiated from the laser nozzle 37. The laser device 16 is supported by a laser base 38 fixed on the base 14 and a height adjustment structure 39 provided on the laser base 38. The height adjustment structure 39 is configured by assembling a plurality of link pairs in an X shape. By rotating the adjustment screw shaft 40 arranged at the upper and lower centers of the link pairs, the height adjustment structure 39 The vertical height of the laser device 16 can be adjusted by changing the crossing angle to large or small.

  The scanning structure 17 is configured as an XY stage with a Y-axis slider 43 fixed on the base 14 and an X-axis slider 44 fixed to a moving table 45 of the Y-axis slider 43. The Y-axis slider 43 and the X-axis slider 44 are each composed of a commercially available ball screw type electric slider, and are arranged in a state orthogonal to each other. The table 21 of the sheet table 18 is fixed to the moving table 46 of the X-axis slider 44. Thus, by supporting the table base 21 with the XY stage provided on the base 14, the table main body 20 can be freely displaced with respect to the laser nozzle 37. Thus, both the Y-axis slider 43 and the X-axis slider 44 are moved according to the preset XY coordinates while irradiating the fluororesin sheets S1 and S2 with infrared laser beams. A weld bead having an arbitrary shape can be formed at the interface between the sheets S1 and S2. In FIG. 11, reference numeral 47 is a control device that controls the operating states of the laser device 16 and the scanning structure 17.

  The cryopreservation bag is manufactured according to the following manufacturing procedure. The laser device 16 adjusts the focus of the condensing lens 36 in advance so as to focus on the interface between the fluorine resin sheets S1 and S2 in which two infrared laser beams are overlapped. As shown in FIG. 10, after opening the sheet pressing body 19 to the standby position, the fluororesin sheets S1 and S2 are placed and positioned in the center of the table body 20, and then the sheet pressing body 19 is swung downward. To the welding position. Next, as shown in FIG. 13, the scanning structure 17 is operated so that the welding start position coincides with the irradiation position of the infrared laser beam, and while the laser device 16 and the scanning structure 17 are simultaneously operated, the fluororesin sheet S 1. The outline bead 1 is formed in a single stroke on the interface of S2.

  1 to 9, the cryopreservation bag sealing device includes a bag clamping device 56 that clamps and fixes the sealing portion 55 (see FIG. 9) of the cryopreservation bag, and a sealing portion 55 that is clamped and fixed by the bag clamping device 56. A laser device 57 for irradiating an infrared laser beam toward the head, a scanning structure 58 for moving and operating the bag clamp device 56, a frame 59 for supporting each of the members 56 to 58, and the like. The frame 59 includes an upper frame 60 to which the bag clamp device 56 and the scanning structure 58 are assembled, and a lower frame 61 that supports the upper frame 60. A laser device 57 and a control device 62 are provided inside the lower frame 61. Is arranged. A horizontally long plate-like scanning base 65 is fixed to the inner surface of the front frame inclined backward, and a scanning window 66 that allows the bag clamp device 56 to move left and right is opened at the center of the upper and lower sides.

  As already described, the cryopreservation bag is filled with a living tissue, and then deaerated, and a seal bead 125 described later is formed in the sealing portion 55 in a state orthogonal to the entrance / exit 3. As shown in FIG. 9, the sealing portion 55 is a sheet region between the upper bead portion 4 and the upper end of the entrance / exit 3, but the seal bead 125 is orthogonal to or intersects with the entrance / exit 3 in the upper and lower central portions of the sealing portion 55. It is preferable to form in such a state.

  1, 4, and 5, the bag clamp device 56 is fixed to the fixed clamping block 67 with a fixed clamping block 67 supported by the scanning base 65 and a pair of guide shafts 68 fixed to the rear surface of the fixed clamping block 67. A movable holding block 69 supported so as to be freely contacted and separated, a solenoid (clamp actuator) 70 (see FIG. 1) for moving the movable holding block 69 to and away from the fixed holding block 67, and the movable holding block 69 forward. It is composed of a compression coil spring 71 or the like that moves and energizes. The fixed clamping block 67 includes a horizontally long block base 73 in which an irradiation window 72 for infrared laser beams is opened, a heat radiator 74 made of a solid material rich in infrared transmission and heat conduction, and a block base 73. And a radiator holder 75 that supports the radiator 74 in a state of facing the irradiation window 72.

  The block base 73 of the fixed clamping block 67 is supported by the clamp table 91 and the vertical adjustment structure, details of which will be described later. The heat radiating body 74 is made of a solid material rich in infrared transmission and heat conduction. As a solid material for forming the radiator 74, any one of zinc selenide, zinc sulfide, and silicon, which is transparent to the infrared laser, can be applied because of performing a welding process using a carbon dioxide gas laser. In the example, the heat radiator 74 is formed in a horizontally long plate shape with single crystal silicon. The radiator 74 is mounted and fixed to the radiator holder 75 in a state in which the front surface of the irradiation slit 76 opened at the upper and lower centers of the steel radiator holder 75 is closed. The front surfaces of the radiator 74 and the radiator holder 75 are flush with each other. The left and right ends of the radiator holder 75 are detachably mounted with second screw bodies (second mounting tools) 77 screwed into the block base 73 (see FIG. 4). The second screw body 77 is composed of a thumbscrew having an operation knob fixed to the end of the screw shaft, and the radiator holder 75 can be attached and detached by rotating the operation knob with a fingertip.

  The movable clamping block 69 is made of an aluminum plate material having high thermal conductivity. As shown in FIG. 1, a pair of left and right sliders 79 supported by the previous guide shaft 68 so as to be slidable back and forth, and both sliders 79. And a pair of left and right block support shafts 81 fixed to the left and right of the front surface of the movable base 80. The block support shaft 81 protrudes forward from a shaft opening 82 (see FIG. 4) formed in the block base 73, and a movable clamping block 69 can be attached to and detached from the front end thereof with a first screw body (first mounting tool) 83. It is attached to. The first screw body 83 is the same thumbscrew as the second screw body 77. In order to facilitate and ensure the attachment and detachment of the sealing portion 55 by the bag clamp device 56, the movable clamping block 69 and the fixed clamping block 67 face the outer surface of the scanning window 66, respectively.

  As described above, the movable clamping block 69 is operated by the solenoid 70 and the spring 71 to move back and forth, and can be displaced between a clamping posture that circumscribes the radiator holder 75 and a standby posture that moves forward from the radiator holder 75. Is held in a standby position by receiving the biasing force of the spring 71. As shown in FIG. 1, the solenoid 70 is mounted on an inner base 84 fixed to the rear end of the guide shaft 68, and an operation shaft 85 continuing to the movable iron core is fixed to the left and right center of the movable base 80. As shown in FIGS. 2 and 3, a foot switch (switch) 86 for operating the solenoid 70 is provided on the front floor surface of the sealing device, and when the switch 86 is depressed, the movable iron core moves into the solenoid 70. The movable holding block 69 can be switched to the clamping posture against the urging force of the spring 71. This clamping posture is maintained until the sealing process is completed. When the foot switch 86 is stepped on again after the sealing process is completed, the movable clamping block 69 is returned to the standby posture by the spring 71.

  As described above, when the movable clamping block 69 is supported slidably back and forth with respect to the guide shaft 68 of the fixed clamping block 67, the movable clamping block 69 can be smoothly moved back and forth with respect to the fixed clamping block 67, and both the clamping blocks can be moved. The parallelism of the clamping surfaces of 67 and 69 can be enhanced. Further, when the solenoid 70 is provided on the inner base 84 fixed to the guide shaft 68, the movable holding block 69 is operated in the front-rear direction by the solenoid 70 having a simpler structure, and the sealing portion 55 is formed by both the fixed and movable holding blocks 67 and 69. Can be securely clamped.

  The scanning structure 58 includes a scanning base 65, guide rails (guide bodies) 89 provided above and below the front edge of the scanning window 66 of the scanning base 65, and a total of four slides that are guided and supported by the guide rail 89 so as to be slidable to the left and right. Body 90, a horizontally long rectangular frame clamp table 91 fixed to the slide body 90, a gate-shaped inner frame 92 fixed to the lower back surface of the scanning base 65, and a left and right horizontally long electric slider fixed to the inner frame 92 (Scanning actuator) 93 and the like. The clamp table 91 is formed in a horizontally long rectangular frame shape, and the table 91 and the moving table 94 of the electric slider 93 are connected by a reverse L-shaped interlocking arm 95. By operating the electric slider 93, the bag clamp device 56 can be scanned and moved from one end of the scanning window 66 to the other end. The movement stroke of the bag clamping device 56 by the scanning structure 58 at this time is set sufficiently larger than the left and right width of the sealing portion 55 of the cryopreservation bag. A touch panel type display 96 is provided in the upper right corner of the front surface of the frame 59. By touching a start button displayed on the display 96, the electric slider 93 is operated, and at the same time, an infrared laser beam is emitted from the laser device 57. Can radiate.

  In order to scan the bag clamp device 56 up and down, a vertical scanning structure is provided inside the frame 59. The vertical scanning structure includes a vertically long guide rail (vertical guide) 97 fixed to the clamp table 91, a vertical slider 98 supported by the guide rail 97 so as to be slidable back and forth, and a bag clamp via the inner base 84 described above. The apparatus 56 is constituted by a servo motor (up / down scanning actuator) 99 for operating up and down. As shown in FIG. 4, the block base 73 of the fixed clamping block 67 is fixed to a pair of left and right upper and lower sliders 98. The servo motor 99 is disposed on the upper surface of the actuator base 100 fixed to the upper inner surface of the clamp table 91, and its rotational power is converted into a reciprocating motion by the screw shaft 101 and the female screw body 102 fixed to the inner base 84. The bag clamp device 56 is moved up and down.

  In FIG. 2, a laser device 57 is a commercially available carbon dioxide laser unit, which is a rectangular box-shaped laser oscillator 104 that is long in the vertical direction, an irradiation tube 105 that protrudes from the upper end of the laser oscillator 104, and a laser oscillator 104. And a condensing lens 107 that squeezes the laser light and irradiates the heat radiation body 74 with the laser light. In order to adjust the focus position of the condenser lens 107, a focus adjustment structure is provided at the rear of the inner frame 92.

  The focus adjustment structure includes a lens base 108 supported by the inner frame 92, a pair of left and right lens guides 109 fixed to the lens base 108, and a front / rear slider 110 supported by each lens guide 109 so as to be slidable back and forth. The lens holder 111 is fixed to the front / rear slider 110 and supports the condenser lens 107, and the servo motor (focus adjustment actuator) 112 that operates the lens holder 111 back and forth. The servo motor 112 is arranged on the lower surface side of the lens base 108, and its rotational power is converted into a reciprocating operation by the screw shaft 113 and the female screw body 114 fixed to the lens holder 111, and the focal position of the condenser lens 107 is changed. adjust.

  In the process of sealing the cryopreservation bag, the infrared laser emitted from the laser device 57 may be leaked. In order to prevent exposure by such leaked laser light, all the peripheral side surfaces and the upper surface of the frame 59 are protective walls. 117. Furthermore, in order to prevent the infrared laser emitted toward the sealing portion 55 from leaking, a protective cover 118 is provided on the front surface of the bag clamp device 56 and can be swingably opened and closed by a bracket 119 and a hinge 120 provided on the frame 59. (Refer to FIG. 1 and FIG. 2). When the cryopreservation bag is attached to or detached from the bag clamp device 56, the protective cover 118 can swing upward and be held in the open position as shown in FIG. 1 and 2, reference numeral 121 denotes a fall prevention plate for preventing the cryopreservation bag after the sealing process from being accidentally dropped on the floor surface.

  The procedure for sealing the cryopreservation bag will be described below. Prior to performing the sealing process, adjustment of the laser device 57 and focus adjustment of the condenser lens 107 are performed to prepare for infrared laser radiation, and the bag clamp device 56 is moved to the home position shown in FIG. Keep it in place. Further, the welding condition is set while checking the display on the display 96, and the standby state is set. In the cryopreservation bag, the housing portion 2 is filled with a living tissue, and further, a deaeration process is performed to flatten the sealing portion 55 into a sheet shape. In this state, as shown in FIG. 6, the sealing portion 55 is inserted between the movable holding block 69 and the radiator 74, and the foot switch 86 is stepped on in the positioned state, and the movable holding block 69 is moved backward by the solenoid 70. To the clamping posture.

  In the state where the movable holding block 69 is in the clamped position, as shown in FIGS. 7 and 8, most of the sealing portion 55 of the cryopreservation bag is held and fixed by the movable holding block 69 and the radiator 74. . In this state, by touching the start button displayed on the display 96, an infrared laser beam is emitted from the laser device 57 toward the heat radiating body 74, and at the same time, the electric slider 93 is operated to bring the bag clamp device 56 into the home position. The seal bead 125 is formed while being fed from the position toward the left side in FIG. 3 at a constant speed.

  At this time, since the radiator 74 transmits the infrared laser, it does not generate heat by absorbing the infrared laser. Further, when the seal bead 125 is formed, welding heat is conducted around the bead forming position, but the welding heat reaching the rear surface of the sealing portion 55 is absorbed by the heat radiating body 74 having excellent thermal conductivity. Diffused. Similarly, the welding heat that has reached the front surface of the sealing portion 55 is absorbed and diffused by the movable holding block 69 having excellent thermal conductivity. Therefore, in the sealing part 55, as shown in the enlarged view of FIG. 7, only the interface of the fluorine resin sheets S1 and S2 is welded.

  When the seal bead 125 is formed from one end of the sealing portion 55 to the other end, the infrared laser emission and the feeding operation by the scanning structure 58 are temporarily stopped, and the servo motor 99 of the vertical scanning structure is operated in this state. The bag clamp device 56 is moved upward (or downward) by the spot diameter of the infrared laser beam. Further, while operating the scanning structure 58 to feed the bag clamp device 56 to the right in FIG. 3, the infrared laser beam is emitted to the sealing portion 55, and below the seal bead 125 formed during the forward path (or A seal bead 125 on the return path side is formed on the upper side. In the state where the bag clamp device 56 has returned to the home position, a double seal bead 125 that reciprocates to the sealing portion 55 is formed as shown in FIG. After that, by stepping on the foot switch 86 while the cryopreservation bag is supported by hand, the clamping action of the sealing portion 55 by the bag clamp device 56 can be released, and the cryopreservation bag can be taken out. The vertical scanning structure returns to the initial state when the bag clamp device 56 returns to the home position or forms the outward seal bead 125 and then moves the bag clamp device 56 in the opposite direction to the previous time. Also good. Thereafter, the sealing beads 125 can be accurately formed in the sealing portion 55 of the cryopreservation bag by repeating the above procedure.

  According to the sealing device configured as described above, the movable holding block 69 is moved and operated by the clamp actuator 70 to clamp the sealing portion 55, and then the scanning structure 58 and the laser device 57 are operated, whereby the fluororesin The entrance / exit 3 of the cryopreservation bag made of S1 and S2 can be automatically sealed. The sealing operation of the sealing portion 55 of the cryopreservation bag is performed by a field user such as a doctor or a medical engineer. After the sealing portion 55 is clamped by the bag clamp device 56, a series of welding operations are automatically performed. As a result, the sealing process of the cryopreservation bag can be performed safely and accurately. In addition, since the sealing process is always performed automatically under certain conditions, there is no variation in the welding results, and therefore, anyone can easily perform a sealing process that can exhibit a high level of sealing function in a stable state for cryopreservation. A bag sealing device can be provided.

  Since the movable clamping block 69 and the fixed clamping block 67 face the outer surface of the scanning window 66, the attaching / detaching operation of the sealing portion 55 with respect to the bag clamp device 56 can be easily performed on the outer surface of the scanning window 66 facing the free space. Therefore, while confirming the position and posture of the cryopreservation bag, the sealing portion 55 can be properly clamped by the bag clamp device 56, or the cryopreservation bag after the welding operation can be reliably collected, and the sealing device as a whole can be recovered. Usability can be improved.

  When the movable clamping block 69 is supported slidably back and forth with respect to the guide shaft 68 of the fixed clamping block 67, the movable clamping block 69 can be smoothly moved back and forth with respect to the fixed clamping block 67, and the both clamping blocks 67 and 69 can be clamped. The parallelism of the surface can be enhanced. Further, when the clamp actuator 70 is provided on the inner base 84 fixed to the guide shaft 68, the movable clamping block 69 is operated back and forth with the clamp actuator 70 having a simpler structure, and sealing is performed with both the fixed and movable clamping blocks 67 and 69. The portion 55 can be securely clamped.

  The movable clamping block 69 is detachably attached to the block support shaft 81 via the first screw body 83, and the radiator holder 75 is detachably attached to the block base 73 via the second screw body 77. It is installed. According to such a sealing device, the movable clamping block 69 and the radiator holder 75 are removed from the block support shaft 81 and the block base 73 as necessary, and the movable clamping block 69 and the heat radiation to which the living tissue may adhere. The body holder 75 can be sterilized. Therefore, the hygienic management of the movable holding block 69, the radiator 74, and the radiator holder 75 can be facilitated, and the mouth treatment of the sealing portion 55 of the cryopreservation bag can be performed in a sanitary and safe state.

  In the above embodiment, the seal bead 125 is continuously formed from one end of the sealing portion 55 to the other end, but it is not necessary. The seal bead 125 only needs to be formed so as to cross at least the entrance 3. In the above embodiment, the return-side seal bead 125 is doubled adjacent to the seal bead 125 formed at the time of the outward path. However, the seal bead is sealed only by the seal bead 125 on either the outward-path side or the return-path side. The part 55 may be sealed. If necessary, the formation pattern of the seal bead 125 can be formed in a continuous waveform or a saw blade shape by operating the vertical scanning actuator 99 in conjunction with the feed movement operation of the scanning structure 58.

  14 and 15 show a modified example of the cryopreservation bag. The transparent fluororesin sheets S1 and S2 stacked in two are irradiated with an infrared laser beam to form a pair of left and right outer wire beads 1 at the interface between the two sheets S1 and S2. Between the sheets S1 and S2 sandwiched between the two, a storage part 132 that stores the living tissue, a vent 133 that continues to the storage 2 and a bleed part 134 that continues to the vent 133 are formed. The end portion of the bleed portion 134 is sealed with a first seal bead 135. The vent 133 is formed at the left and right center of the bag wall.

  The accommodating portion 132 is partitioned by a pair of left and right left bead portions 136 and a right bead portion 137, and shoulder bead portions 138 that incline in a tapered shape from both the bead portions 136 and 137 toward the vent hole 133. The upper end portions of the left and right bead portions 136 and 137 intersect the short side portions of the fluororesin sheets S1 and S2 in an orthogonal manner, and the living tissue is filled between the bead portions 136 and 137 in the accommodating portion 132. A filling port 139 is opened. The extraction part 134 is formed in a funnel shape. As shown in FIG. 15, the lateral width of the bleeder 134 is sufficiently larger than the lateral width of the vent 133, and the lateral width of the accommodating part 132 is set to be larger than the lateral width of the bleeder 134.

The cryopreservation bag configured as described above performs a sealing process after the living body tissue such as blood is filled in the storage portion 132, and a series of operations are performed according to the following procedure.
First, as shown in FIG. 15, after opening the filling port 139 upward, the living tissue is filled from the filling port 139 into the accommodating portion 2 (first procedure). At this time, since the living tissue can be filled in a state where the filling port 139 having the same width as the containing portion 132 is opened, the filling and injection of the living tissue into the containing portion 2 can be performed easily and quickly. Next, the bag wall on the filling port 139 side is brought into close contact with the air inside the housing portion 132 while pushing out the air in the housing portion 132, and the second filling port 139 side of the cryopreservation bag filled with the biological tissue is shown in FIG. Sealing with the seal bead 140 (second procedure). The container 2 in this state often contains a little air, and in order to release this air, the bag wall is cut along the cutting line 141 along the first seal bead 135, The bag wall including the one seal bead 140 is separated and removed, and the extraction opening 142 is formed near the end of the extraction unit 4 (third procedure).

  The cryopreservation bag in which the bleed opening 142 is formed is raised and held in a state where the bleed portion 134 is at the top and the storage portion 132 is at the bottom. Next, the living tissue in the storage part 132 is pushed out from the vent 133 to the extraction part 134 to raise the liquid level, and further, air is released from the extraction opening 142 while closely contacting the bag wall of the extraction part 134 (fourth procedure). . At this time, all the air in the bleed part 134 can be reliably discharged by discharging air from the bleed opening 142 while closely contacting the bag wall of the wide bleed part 134. Further, the temporary sealing portion 143 can be formed by closely contacting the bag wall from the bleed portion 134 to the upper portion of the accommodating portion 132. In this state, by sealing the temporary sealing portion 143 with the third seal bead 144 (fifth procedure), only the living tissue can be enclosed in the accommodating portion 132.

  According to the above-described cryopreservation bag, since the living tissue can be filled with the wide filling port 139 opened widely, filling and injection of the living tissue into the accommodating portion 132 can be performed easily and quickly. . In addition, the living tissue in the container 2 can be pushed out from the vent 133 to the wide bleed part 134 to raise the liquid level, and air can be discharged from the bleed opening 142 while the bag wall of the bleed part 134 is closely attached. The deaeration work of the air that has entered the housing part 132 can be performed easily and quickly. The first seal beads 135 can be formed in the process of manufacturing the cryopreservation bag, but the second seal beads 140 and the third seal beads 144 are sealed using a sealing device.

  In addition to the above, the clamp actuator 70 does not need to be a solenoid, and may be configured by an electric slider, an electric cylinder, a linear actuator, or the like. Similarly, the scanning actuator 93 may be configured by an electric cylinder, a linear actuator, or the like other than the electric slider. Further, the vertical adjustment actuator 99 and the focus adjustment actuator 112 may be configured by an electric slider, an electric cylinder, a linear actuator, or the like. The laser oscillator 104 can be arranged in a horizontally long posture or a long posture in the front-rear direction. The sealing device according to the present invention is particularly suitable for sealing a sealing portion of a cryopreservation bag formed of fluorine-based resin sheets S1 and S2, but the sealing process is formed of a resin sheet other than the fluorine-based resin sheet. Therefore, it is not limited that the cryopreservation bag to be sealed is formed of the fluororesin sheets S1 and S2.

55 Sealing Portion 56 Bag Clamp Device 57 Laser Device 58 Scanning Structure 59 Frame 65 Scanning Base 67 Fixed Clamping Block 69 Movable Clamping Block 70 Clamp Actuator (Solenoid)
74 Radiator 91 Clamp Table 93 Scanning Actuator (Electric Slider)
107 Condensing lens 125 Seal bead

Claims (5)

A bag clamp device (56) for holding and fixing the sealing portion (55) of the cryopreservation bag, and a laser device (57 for irradiating an infrared laser beam toward the sealing portion (55) clamped and fixed by the bag clamp device (56) ) and comprises a frame (59) supporting each member of the upper Symbol (56-57),
Bag clamp device (56) comprises a frame (59) to the supporting lifting is the solid Teikyoji block (67), with respect to the fixed clamping block guide shaft provided on the fixed clamping blocks (67) (68) (67) A movable clamping block (69) supported so as to be freely contactable and separable, and a clamp actuator (70) for operating the movable clamping block (69) to and from the fixed clamping block (67),
The laser device (57) includes a laser oscillator (104) and a condensing lens (107) that condenses the infrared laser beam emitted from the laser oscillator (104) toward the sealing portion (55) of the cryopreservation bag. Including
The fixed clamping block (67) includes a block base (73) in which an irradiation window (72) for infrared laser beams is opened, and a heat radiator (74) made of a solid material rich in infrared transmission and heat conduction. And a radiator holder (75) mounted on the block base (73) and supporting the radiator (74) in a state facing the irradiation window (72),
In a state where the sealing portion of the cryopreservation bag (55) was sandwiched and fixed de fixed clamping blocks (67) and the movable clamping block (69), by irradiating the infrared laser beam to the sealing portion (55), the doorway (3 A sealing bead for a cryopreservation bag, wherein a sealing bead (125) is formed in the sealing part (55). The movable clamping block (69) includes a pair of left and right sliders (79) supported so as to be slidable back and forth by a guide shaft (68), and a plate-like movable base (80) fixedly supported by both sliders (79). And a pair of left and right block support shafts (81) fixed to the left and right of the front surface of the movable base (80),
The inner base (84) fixed to the guide shaft (68) is provided with a clamp actuator (70) for operating the movable clamping block (69) back and forth via the movable base (80) and the block support shaft (81). ,
With the sealing portion (55) of the cryopreservation bag placed on the outer surface of the radiator (74) of the fixed clamping block (67), the movable clamping block (69) is operated with the clamp actuator (70) to The sealing device for a cryopreservation bag according to claim 1, wherein the sealing portion (55) can be clamped and fixed by both movable clamping blocks (67, 69). The movable clamping block (69) is detachably mounted on the block support shaft (81) via the first mounting tool (83).
The radiator holder (75) is detachably attached to the block base (73) via the second attachment tool (77).
The sealing device for a cryopreservation bag according to claim 2, wherein the movable holding block (69) and the radiator holder (75) can be removed from the block support shaft (81) and the block base (73) and sterilized. A focus adjustment structure for adjusting the focal position of the condenser lens (107) is provided inside the frame (59).
The focus adjustment structure includes a lens base (108) supported by an inner frame (92) fixed to the frame (59) , a lens guide (109) fixed to the lens base (108), and a front and rear by a lens guide (109). A front / rear slider (110) supported slidably, a lens holder (111) fixed to the front / rear slider (110) to support the condenser lens (107), and a focus adjustment actuator for operating the lens holder (111) front / rear (112)
The bag sealing device for a cryopreservation bag according to claim 2 or 3, wherein the focal position of the condenser lens (107) can be adjusted according to the thickness of the sealing portion (55) clamped and fixed by the bag clamp device (56). The outer surface of the frame (59) is covered with a protective wall (117) that prevents exposure by an infrared laser leaking from the laser device (57),
The sealing device for a cryopreservation bag according to any one of claims 1 to 4, wherein a protective cover (118) for preventing exposure by an infrared laser is provided on the outer surface of the bag clamp device (56) so as to be openable and closable.

Images