JP2011234706A - Method for simply evaluating microorganism risk and functional space avoiding the problem - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a method for simply evaluating a potential microorganism risk of a space according to progressing aspect of the deterioration of organic substances in the limited circumference or the space relative to the problem caused by the microorganisms generated in the natural circumference and to provide a functional space avoiding the microorganism risk and a functional member for constituting the functional space using the above evaluating method.SOLUTION: The evaluating method of the microorganism risk for observing the deterioration condition by arranging the organic substances such as foods or plants in the space constituted by three-dimensionally assembling members comprising woods, paper, plastics, clays, sintered clay matters, glass, metals, cement, stones, woven fabrics, minerals, fibers, paints. The functional member is constituted by blending a suspension constituted by decomposing the organic substances by the microorganisms secured in safety used for the foods in a manufacturing stage as the above members as the solving technique to the deterioration of the organic substances, the method for manufacturing the functional members is disclosed. The functional space is constituted by three-dimensionally assembling the functional members.

Description

  The present invention relates to a method for judging a potential microbial risk of a space as a structure based on a deterioration state of organic matter, a functional space for delaying the deterioration of organic matter, and a functional member constituting the functional space. It relates to a manufacturing method.

  Problems caused by microorganisms include health problems such as food poisoning, nosocomial infections, health damage due to the emission of sick house causative substances, diseases such as crops and livestock, food corruption, and deterioration of buildings. Among the microbial damage, the cause of the direct health damage to humans and animals has been investigated. However, in buildings, the standards related to disaster prevention are more important than microorganisms, and food that has been purchased by consumers can be discarded or recycled if it decays. It cannot be said that proper treatment is performed like microorganisms that affect living bodies.

  This difference in handling is born depending on the type of microorganism. Pathogenic microorganisms are stipulated by the Infectious Disease Law and the National Institute of Infectious Diseases pathogen safety management regulations, etc., and are appropriately managed according to the degree of risk. However, there is a risk that microorganisms may suddenly become harmful individuals due to mutations, natural gene exchange, etc. In other words, because an environment in which microorganisms are easy to propagate can cause the growth of pathogenic microorganisms that infect humans, there is a biological risk against the decay of organic matter observed on a daily basis. Don't be.

  Next, the principle of organic decay and general countermeasures will be described. Organic matter deteriorates under the influence of light, heat, oxides, etc., and is finally decomposed by microorganisms. The control method of microorganisms is a combination of sterilization technology and storage technology. As sterilization technology, heating, UV, microwave, radiation, etc., storage technology includes low temperature, freezing, freezing, pressurization, nitrogen filling, humidity adjustment, There is a method of directly controlling with useful microorganisms such as biopreservation, such as direct addition of bactericides. If sufficient sterilization is performed, the microorganisms can be controlled during the storage period, but if the storage conditions are poor or the storage state is released, the organic matter is spoiled by the influence of the microorganisms.

  The simplest storage method is to use a container, and it is preserved by combining minerals such as tourmaline, metals such as silver and copper, functional ingredients, and biological resources such as microorganisms in a commonly used storage container. Functional containers that have been devised to improve the performance are also commercially available. However, because the basics of functional raw materials are effective when they come into contact with each other, it is not possible to take measures when microorganisms have propagated in organic matter, even if measures can be taken in contact with the container. .

  There are also many problems with explanations related to preservability. For example, with regard to negative ions, which are considered to be one of the effects of minerals, weak radiation may be used, and there is a question about safety during production and use. Metals also have high rarity value, and their use in discarded containers can be said to be a means that does not match the direction of resource saving. On the other hand, there are methods that use biological resources, which are considered desirable from the viewpoint of environmental measures. However, they are highly dependent on temperature, etc., and it is certain that biological components will remain in the product and develop functions. There is no good argument.

  For example, as shown in Patent Document 1, even if an effect is expressed by adding biological resources, there is a possibility that the antimicrobial property may be an action caused by an oxide such as ceramics, or a resin containing an organic substance is heated. The possibility of pores formed by foaming due to processing is also expected, and if the added biological resource itself cannot be separated and extracted, there is also a problem that it is not possible to prove that the original function of the biological resource was expressed. .

  In addition, in an enzyme-like example, the reaction occurs when the active center and the substrate specifically bind to each other, but the functional expression requires direct interference with the target, If the functional component is mixed, the active site is not exposed, and if the contact with the object does not occur, the functionality cannot be expressed. Furthermore, the biological component is a substance for maintaining a life phenomenon, and is rarely deactivated and effectively acts outside a normal environment.

  As for the method of directly using microorganisms, we expect the effects of microorganisms to survive in the product, but proteins and enzymes produced by microorganisms are vulnerable to physicochemical stress as described above. Even though biological remains such as diatomaceous earth contribute to the structural and physical functions of the product, it is not considered that the functionality of microorganisms, microorganism-generated substances, etc. is manifested as product functions.

  Although there are many problems with biological resources as described above, in recent years there have been reports that traces of the presence of microorganisms have been confirmed from the hot water near the submarine crater, and it is necessary to review the theories regarding the survival conditions of microorganisms. Is coming out.

  On the other hand, if a microorganism that has not been discovered in the past is newly detected and used, it is also necessary to assume a new microorganism risk. However, known microorganisms are considered to be about 1% of the microorganisms that exist in nature, and it is impossible to take measures to investigate the cause of all of 99% of microorganisms that may be detected in the future. It can be said.

  Furthermore, unexpected events have been confirmed as microbial damage. For example, in the case of Takamatsuzuka burial mound shown in Non-Patent Document 1, as a cause of wall deterioration, the acrylic resin itself mixed in the plaster at that time, the test results indicating the possibility that mold was easy to propagate has been published In the future, there may be more reports on unexplained microbial damage.

  As described above, the effects of unknown microorganisms and traces of microorganisms are not stipulated in conventional quality control standards, and microbial inspection only at a fixed point during production does not lead to an increase in safety in the product use environment.

JP 2005-95633 A

Resistant to mold of resin used in ruins

  The present invention, as a countermeasure against unspecified microbial risk, to evaluate the degree of microbial propagation in the simulated space, to detect in advance the potential microbial risk that occurs after forming a space by a plurality of products, and Provide functional space for avoiding microbial risks that have been improved based on the evaluation results, and a method for manufacturing functional members that avoids microbial contamination for constructing functional spaces, and damage microorganisms from spatial components Establishing a healthy space with reduced safety and ensuring safety is an issue to be solved.

(Risk assessment method)
The risk assessment for solving the above-mentioned problems includes a structure (I) having a hollow inside alone, a structure (II) formed three-dimensionally using the product as a member, and a single ( I) or a composite structure (III) in which different products are molded by covering, coating, sticking, or standing on the inner surface of a structure (II) molded three-dimensionally using products as members. .

  In addition, the product constituting the structure or the product composite must constitute at least one surface of the ceiling, wall, or floor, the interior is entirely isolated from the outside, or partly open. It is necessary to be surrounded as a whole.

  The above products and product composites are made of wood, paper, plastic, clay, fired clay, mineral, glass, metal, cement, stone, fiber, paint, etc., alone or in combination. It is characterized in that it can constitute a surface or has a property that can be adapted to a structure when it is coated, applied, or left standing.

  And inside the structure, foods that have moisture such as cereals, fresh products, fruits, meats, breads / confectionery, beverages, etc., plants such as vegetables, flowers, etc. are inserted in water and kept in contact or non-contact state. The ability of the space itself to deteriorate can be evaluated by observing the progress and observing changes such as the occurrence of mold and discoloration with the naked eye.

  Furthermore, the results of the present invention can be used as a method for determining the degradation state of organic matter, and serve as an evaluation method for the development of functional members that delay the degradation of organic matter and functional members that constitute the functional space. Can do.

(Functional space)
Before disclosing the functional space of the present invention, it is necessary to define the definition of the space in the present invention. In the first place, the word space is defined in mathematics and physics. In terms of words, it is written as a place where there is nothing in the dictionary, and there is no recognition as a unique object. However, terms such as living space are used everyday, and the space intended by the applicant also refers to a specific environment used daily.

  Next, interpret the word space for everyday use. A space is a word composed of a sky (sora) and a space (ken). The sky is a real and generally recognized surrounding environment, and the space is a unit of length unique to Japanese culture. In other words, the meaning of the word space can be recognized as a partial environment obtained by dividing an existing environment at a certain scale, and it can be assumed that it is a three-dimensional structure having a cavity. That is, the space generally referred to indicates a structure having a cavity inside.

  And the space in this invention intends the structure shape | molded by the said product, and functional space will be comprised similarly. That is, the functional space of the present invention includes the structure (I) having a cavity inside the product alone, the structure (II) formed three-dimensionally using the product as a member, and the structure (II) alone or three-dimensionally using the product as a member. It is a complex structure (III) in which different products are coated, coated, pasted, left standing, etc. on the inner surface of the structure (II) that has been molded into a structure (III), and the structure itself causes deterioration of organic matter. It has the performance to delay.

  Further, in the functional space of the present invention, the product forming the space is composed of a functional member, and the functional member needs to constitute the whole or a part of the ceiling surface, the wall surface, and the floor surface.

(Method for producing functional member)
The functional member of the present invention is a member for space formed by mixing, rolling, extruding, etc., materials such as wood, paper, plastic, clay, fired clay, mineral, glass, metal, cement, stone, fiber, paint, etc. In addition, adding a functional ingredient as a raw material at the manufacturing stage, and that the functional ingredient added to the functional member is a suspended liquid or solid that has been proliferated by organically decomposing organic matter by microorganisms, Features.

  The reason for adding suspensions containing the above microorganisms is generally assumed that organic matter deterioration in the space is due to (1) the organic matter itself and (2) microorganisms that have entered from outside the space and proliferated. However, the applicant predicted that the deterioration of the organic matter is induced by the member by adding the possibility of (3) the structural member and the attraction of rot by the space composed of the structural member to the cause on the left.

  In other words, in the manufacturing stage, the product contains a lot of microorganisms in the natural environment, and the contaminated microorganisms may not always be detected by conventional detection methods, or are not detected by sterilization treatment in known microorganisms. Even if the microbial cells and spores remain, there is a possibility that the growth can be resumed when the conditions are met, and even if naturally contaminated microorganisms are sterilized, the properties of the product are changed. If there is no research that can deny the possibility of unexplained that there is a possibility of inducing deterioration of the organic matter, and if the organic matter is installed in the space constituted by the members contaminated with microorganisms for the above reasons, The possibility of accelerating the deterioration of organic substances by directly or indirectly receiving the influence of the material to be used cannot be denied. Te, the addition of beneficial microbes as a raw material, a situation in which the dominant than the natural contaminating microorganisms, artificially there is a need to create.

  Next, the selection conditions for microorganisms to be functional components of the present invention are as follows. In order to ensure safety in use by the microorganisms, the microorganisms must be at least harmless microorganisms used for food processing, Like microorganisms that can grow under conditions and microorganisms that form durable spores, they may remain even after sterilization and are not a concern for contaminating production lines This was the standard for selection.

  In addition, it is desirable to use a microorganism that has been confirmed to be killed or undetected by a general sterilization treatment because the source of the microorganism is clear. It can be said that the action of adding the above microbial suspension as a raw material is a necessary measure in order not to become a new microbial risk.

  However, sterilization with chemical substances alone is meaningless unless it is compatible with unknown microorganisms that are expected to be mixed. Furthermore, there is a risk of adverse effects on the living body due to volatilization and elution after molding, and the creation of resistant bacteria. In addition, there is a possibility that only the above-mentioned microorganisms may be killed, so it is not necessarily suitable for the present invention, and even when used in combination, it is used within a range that does not significantly inhibit the survival of the raw material microorganisms in the present invention. It is necessary to keep in quantity.

  And the dominance of microorganisms is realized by making a numerical advantage by the microorganisms or by using microorganisms having properties excellent in nutrient competition.

  As a method of making a numerical advantage, it is necessary to add more microorganisms than the resident microorganisms at the production site, but the number of microorganisms in the environment outside the production site using organic materials is very small. When microorganisms grown in the manner of the present invention are added, since even a small amount is dominant, there is no need to pay much attention to the amount added.

  As a condition of microorganisms that have excellent nutritional competition, it is a species that can survive in the natural environment, as well as microorganisms that are expected to be naturally mixed into products. Among microorganisms used in food processing, other than artificial media As a nutrient source of food, it is possible to assimilate saccharides contained in foods in a state where proteins and lipids are turbid, and when the number of bacteria larger than naturally mixed microorganisms is ingested into liquids or solids with turbid organic matter, The selection condition was that the ratio of the number of bacteria was greater than that of the contaminating microorganism, and that it could dominate. Depending on the type of microorganisms that have been artificially cultured, the ability to decompose natural organic matter may be reduced, and microorganisms that have a slow growth rate in the natural environment are disadvantageous for survival competition. It will not be suitable as a raw material microorganism to avoid microbial risk.

  In general, microbial inspection of products does not indicate the degree of microbial contamination in the production stage, but the number of microorganisms after sterilization is inspected, so the influence of naturally contaminated microorganisms outside the product is not assumed. However, since the microbial risk after the formation of the space can be detected by the microbial risk evaluation method of the present invention, it contributes to the formation of a space that realizes higher safety.

  In addition, according to the evaluation method, even in a space formed by members using the same raw material, the quality of the member may change depending on the manufacturing method, raw material grade, and other factors, and the effect on the organic matter installed in the space may change. Since it can also detect the property, it can be used as a standard for product development.

  And based on the result of the said evaluation, in the functional space which consists of an improved functional member, a microorganism risk is avoided and the deterioration of the organic substance stored in the said space will be delayed.

  Furthermore, since the functional space is physically replaced by naturally mixed microorganisms in the manufacturing stage of the member by beneficial microorganisms that are raw material microorganisms, the inside of the structure constituted by the member is avoided. On the other hand, the expansion of contamination from the member will be eliminated, and as a result, if it is in the space, it becomes possible to obtain the effect of delaying deterioration no matter where the organic substance is installed, and particularly contact with the functional member There is no need.

  In addition, the functional member according to the present invention does not affect the organic matter of the raw material microorganisms, and is the performance of the member itself obtained by eliminating the spoilage property by adding the raw material microorganisms. Even if the functional member alone is used, the organic substance is weakly affected, and a weak effect can be confirmed without forming a three-dimensional space.

It is explanatory drawing which showed the kind of closed space. It is explanatory drawing which showed the space where one part was open | released. It is explanatory drawing which showed the kind which comprises space. It is explanatory drawing which showed the arrangement | positioning condition of the organic substance for risk evaluation. It is explanatory drawing which showed the installation position of the functional member with respect to functional space. It is explanatory drawing which showed the structural example of the functional member which combined the functional gypsum board, the functional paste, and the functional vinyl chloride wallpaper. It is explanatory drawing which showed a mode that the organic substance was installed in the simulation space and it is testing. It is explanatory drawing which shows a mode that the raw material microorganisms were added to calcium carbonate and preserve | saved. It is explanatory drawing which showed a mode that it constituted the simulated space by the functional diatomaceous earth interior material and the wallpaper made from a general vinyl chloride, and was tested. It is explanatory drawing which showed a mode that the simulated space by the functional vinyl chloride wallpaper and the general vinyl chloride wallpaper was comprised, and it tested. It is explanatory drawing which showed the state of the bell pepper in the 45th day in the simulation space by a functional vinyl chloride wallpaper and a general vinyl chloride wallpaper. It is explanatory drawing which showed the state of the 12th day which investigated the direct influence on the fresh flower of a raw material microorganism addition aluminum piece and an additive-free aluminum piece. It is explanatory drawing which showed the growth condition of the 3rd day after harvesting the mung bean grown for 5 days with the raw material microorganism addition resin container and the additive-free resin container. It is explanatory drawing which showed a mode that the natto of the same pack was divided | segmented into each bag and preserve | saved in the refrigerator for two months in the raw material microorganism addition bag and the additive-free bag. It is explanatory drawing which showed the mode two years after the water stretched in the raw material microorganism addition coating agent coating tank and the additive-free coating agent coating tank.

  Next, the details of the present invention will be described with reference to examples and spatial configuration examples.

(Risk assessment method)
In the present invention, the structure (1-A, 1-B) that is entirely covered and the structure (2) that is partially open are defined as spaces.

  And when assessing microbial risk, the space that is completely isolated (3-A), the space that is partly open but covered by other materials (3-B), and the actual part that is partly open A space (3-C) shielded by the floor is prepared.

  In the interior of the space, a method of installing organic matter directly on the floor surface (4-A), a method of placing organic matter on a table or vessel (4-B), a method of hanging organic matter from the ceiling surface (4) -C), and as long as organic matter is installed in the space, there is no need to select the installation location.

  The type of organic substance is not limited as long as it is food, and typical examples include processed products such as vegetables, meat and bread, beverages, etc., and the same production lot number or production date is used for the test. In addition, it is possible to use as a specimen a flower color change due to oxidation, such as a plant such as a flower, but when using a plant, it is a different strain than that separated from the same strain, If you do not use those that have flower buds differentiated at the same position and at the same time, you will not get accurate test results.

Next, a configuration method for functionalizing the space described in the first embodiment will be described.
(Functional space)
The functional space according to the present invention is constituted by a functional member having a function of delaying the deterioration of organic matter. And about the installation location of a functional member at the time of shaping | molding simulated space, it applies to the whole independently (5-A), it combines and uses a functional member (5-B), a functional member and normal (5-C) which uses the member of (2) together.

  Based on the microbial risk evaluation method according to Example 1, the functional space formed by the functional member observes the state in which the organic matter deterioration is delayed. However, as a preliminary test, changes such as fading can be observed by installing a functional member alone on the floor and placing a plant or the like on the functional member.

Next, the manufacturing method of a functional member is demonstrated.
(Test 1)
An example is shown in which microorganisms are propagated on calcium carbonate, which is a kind of raw material for functional members. Add a suspension of non-sterile sugarcane molasses to a suspension containing mainly lactic acid bacteria and cultivate a microorganism suspension (hereinafter referred to as the starting microorganism) until the calcium carbonate becomes a slurry. As a result, mold was generated in the calcium carbonate added with microorganisms (8-2) as shown in FIG.

  Drying at room temperature is inadequate, and humidity is maintained in a highly sealed container, so that molds that exist in any of the packages propagate using organic matter contained in the microbial suspension as a nutrient source. It is thought that it was because. That is, even if the raw material microorganisms selected in the present invention are used, it is necessary to pay attention to the fact that simply ingesting cannot suppress the spoilage, and conversely, the bacteria can be propagated.

(Test 2)
Next, the diatomaceous earth used as the raw material of a functional member was improved. Add the raw material microorganisms used in Test 1 to the extent that the diatomaceous earth is completely moistened, naturally dried diatomaceous earth that has been naturally dried, and mechanically dried diatomaceous earth that has been dried to a moisture content of 1% or less. When the mold was observed in an artificial medium, mold was not generated in the machine-dried diatomaceous earth. The drying of machine-dried diatomaceous earth was outsourced.

(Test 3)
Test 2 An example in which a test space for risk evaluation was constructed and tested using diatomaceous earth added with the raw material diatomaceous earth of Composition 2 and the raw material microorganism described in Prototype 1 and general vinyl chloride wallpaper is shown. The space is formed of plywood and the interior is compounded with the building material. The foods placed inside are milk, bread and meat. After the start of evaluation, in the space complexed with vinyl chloride wallpaper, mold was observed on the bread with the naked eye, but in the space complexed with diatomaceous earth, mold was not observed on the bread. The meat was dry and there was no difference. Each milk had a slight odor, but there was a difference in odor.

  Diatomaceous earth originally has humidity control performance, and mold growth is suppressed when the humidity in the environment is kept low by using diatomaceous earth. Furthermore, if an alkali raw material such as slaked lime is blended in diatomaceous earth and the inside of the diatomaceous earth is kept alkaline, the pH of mold growth is not reached, and mold growth itself can be suppressed. In addition, as in the present invention, by adding fermentation microorganisms such as lactic acid bacteria as raw material microorganisms, microorganisms in diatomaceous earth become more healthy and contribute to the formation of an environment in which organic matter is not easily spoiled.

(Test 4)
An example of improving the vinyl chloride wallpaper will be described. The structure is formed by pasting the material made by forcibly drying the clay impregnated with the microorganisms shown in Configuration Example 2 into the surface of the space made of general gypsum board, and adding the vinyl chloride wallpaper molded at the manufacturing stage. The microbial risk was evaluated. The arranged food was meat, bread and peppers, and the meat and bread were dried before rotting and did not change, but the peppers started to discolor in about 10 days, and finally only the additive-free wallpaper was moldy. The occurrence of was confirmed.

(Test 5)
The example which added the raw material microorganisms to the metal is shown. The microorganisms shown in the configuration example 2 were wiped, a sufficiently dried aluminum ingot was melted, and a raw material microorganism-added aluminum piece cut out by cooling and an additive-free aluminum piece prepared in the same manner were prepared and immersed in water, respectively. . Two immersions of flower bud differentiation were inserted in the immersion water, and the progress was observed. The additive-free aluminum piece started to petal browning on one side after the 10th day. In the raw material microorganism-added aluminum piece, no change was observed until the 12th day, and on the 14th day, a change such as browning was observed at the same time.

(Test 6)
An example of utilization of raw material microorganisms will be described. When the raw material microorganisms are impregnated with clay, etc., mixed with resin etc. in a sufficiently dry state to prevent mold generation, and a container with a space inside is molded, even if some are not shielded, the contents In the case where the interior of the space is not exposed by filling, the effect is exhibited.

  For example, when mung beans are filled in a container molded with resin containing raw material microorganisms, grown in a dark place, and an environment shielded from the outside by sprouts is formed, no additives are added at the same time as the sprouts Compared to the growth situation of the sprouts cultivated in the container, after separating from the container, the cultivated sprouts with added raw material microorganisms developed cotyledons and further grown, whereas the sprouts with additive-free cultivated sprouts rot in about 2 days Started.

(Test 7)
In addition to the above tests, the effect of delaying the decay of organic matter has been confirmed even in polyethylene bags that individually form cavities.

(Test 8)
In addition, although it is not the deterioration of organic matter, the FRP surface is combined with a coating agent added with raw material microorganisms, and it is left in a state where water is pasted inside the tank where the space is formed by three-dimensionalization, As a result of observing changes in water quality, the raw material microorganism added coating agent coating tank showed almost no scale build-up even after 2 years. Corrosion was also observed.

  Since the present invention can be applied to all structures having an internal structure, it can be used in all industrial fields related to organic matter, but is particularly useful in the field of architecture and food processing.

1-A Cubic Structure 1-B Spherical Structure 2 Partially Opened Structure 3-A Sealed Spatial Configuration 3-B Partially Opened Spatial Configuration Sealed with Film 3-B-1 Films 3-C Space configuration in which the open surface of a partially open structure is installed on the floor surface and sealed 4-1 Floor surface 4-2 Organic matter 4-A Test example 4-B in which organic matter is directly installed on the floor surface Test Example 4-B-1 Stand, etc. with Organic Material Installed 4-C Test Example 4-C-1 String Hanging Organic Material Hanging from Ceiling Surface 5-1 Functional Member 5-2 Functional member 5-A Space 5-B formed with a single functional member Space 5-C formed with a plurality of members, all of which are functional members Partially formed with a plurality of members that are not functional members Space 6-1 Functional gypsum board 6-2 Functional paste 6-3 Functional vinyl chloride wallpaper 8-1 Calcium acid 8-2 Calcium carbonate added with raw material microorganisms 9-1 Functional space composed of functional diatomite interior material 9-2 Space composed of general vinyl chloride wallpaper 10-1 Constructed with functional vinyl chloride wallpaper Functional space A
10-2 Functional space B composed of functional vinyl chloride wallpaper
10-3 Space 11-1 made up of general vinyl chloride wallpaper 11-1 Food placed in functional space A made up of functional vinyl chloride wallpaper 11-2 Made up of functional vinyl chloride wallpaper Food placed in functional space B 11-3 Food placed in space composed of general vinyl chloride wallpaper 12-1 Flowers inserted in water (negative control)
12-2 Flower inserted in water soaked with no added aluminum pieces (positive control)
12-3 Flower inserted in water soaked with raw material microorganism added aluminum piece 13-1 Mung bean 13-2 cultivated in raw material microorganism added resin container 14-1 Mung bean cultivated in additive-free resin container In raw material added microorganism resin bag Preserved natto 14-2 Natto 15-1 preserved in additive-free resin bag Water in raw material microorganism-added coating agent coating tank 15-2 Water in additive-free coating agent coating tank

Claims (5)

  A structure that has a single cavity inside a flat or spherical molded product made of wood, paper, plastic, clay, fired clay, mineral, glass, metal, cement, stone, fiber, paint, etc. A structure in which a plurality of products are three-dimensionally molded as members, or a different product is coated, coated, affixed, or left standing on the inner surface of a single or a plurality of products three-dimensionally molded as members. Prepare any kind of complex structure, place organic substances such as food and plants in the internal space of the structure, and determine the potential microbial risk by observing the occurrence of mold with the naked eye Microbial risk assessment method for space characterized by   A structural member improved to form a space for delaying the deterioration of organic matter based on the test result by the microbial risk evaluation method according to claim 1, wherein the member itself is directly on the organic matter as a simple substance or a complex. Functional member characterized by exhibiting a function of delaying deterioration without contact   A functional space comprising the structure according to claim 1 by the functional member according to claim 2.   In the manufacturing stage of the product used as a component, beneficial microorganisms, mainly lactic acid bacteria containing organic matter suspensions, are added as raw materials, and the microorganisms dominate and replace naturally contaminated microorganisms, thereby avoiding microbial contamination of components. The method for producing a functional member according to claim 2, wherein microbial damage in the space by the member is avoided.   The microorganism according to claim 4, which is a kind used in foods, and cannot be detected from the product by a normal sterilization method in the production stage of the product.