JP2010126484A - Antimicrobial composition - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an antimicrobial composition having sufficient antiseptic effect, low in irritancy and high in safety, for use in plant-derived cosmetics. <P>SOLUTION: The antimicrobial composition, with transparent appearance, includes 1,3-propanediol and/or a 5-10C 1,2-alkanediol and a fermentation-transformed product which is obtained by extraction of plants belonging to the genera Scutellaria baicalensis, Camellia sinensis, Compositae, Houttuynia cordata and Citrus junos and then subjecting the resultant extract to actinomyces and lactic acid bacteria. A cosmetic composition containing the antimicrobial composition is also provided. <P>COPYRIGHT: (C)2010,JPO&INPIT

Description

  The present invention relates to an antibacterial agent composition suitably used for cosmetics.

  Conventionally, preservatives such as benzoic acid esters, phenoxyethanol, benzoic acid and salts thereof, sorbic acid or chlorhexidine gluconate have been used as an antiseptic system for cosmetic compositions. However, some of these preservatives are known to be irritating and allergic to the skin and have safety problems and have been suspected as environmental hormones that have become problematic in recent years. Some are. In addition, these preservatives are limited in the types of bacteria that can inhibit the growth, for example, benzoic acid ester has a high antibacterial effect on bacteria, but has a low antibacterial effect on fungi and yeast. Has drawbacks. In the case of suppressing bacterial growth only with sorbic acid or benzoic acid ester, it is necessary to add a preservative to the cosmetic composition at a high concentration, which may impair the safety of the cosmetic composition and lead to skin irritation. is there. In particular, in a water-based cosmetic composition, there are many cases where the antiseptic effect is not sufficiently obtained, for example, when the preservative is reacted or inactivated with the cosmetic composition substrate and the partition coefficient to the aqueous phase is small.

  Moreover, various organic acids, glycine and the like are known as those having a natural antiseptic effect, but all have a low antiseptic effect, regardless of the bacterial species that cause microbial contamination of the cosmetic composition. There has been a strong demand for antibacterial substances having antiseptic effects. At present, there is a tendency to demand cosmetic compositions composed of natural materials, and in particular, antibacterial substances derived from natural products are demanded.

As a natural antibacterial substance, for example, Patent Document 1 discloses that dimethoxytetrahydroxyflavone, baicalin and ougonin, which are flavonoids extracted from plants such as hornon, have antibacterial activity.
JP 2004-59566 A

  The present invention is a natural antibacterial substance having a transparent appearance and has a high antibacterial effect against bacterial species such as bacteria, fungi and yeast that cause contamination of the cosmetic composition. An object of the present invention is to provide a cosmetic composition excellent in safety capable of obtaining a sufficient antiseptic effect.

  The present invention has found that a fermentation conversion product mixture obtained by biotransforming a specific plant extract has a sufficient antiseptic effect and is highly safe with low irritation. Such a fermentation conversion mixture has a separated opaque appearance, but it has been found that it can be made transparent by blending a specific alcohol, and the present invention has been completed.

  That is, the present invention provides an extract from a plant belonging to the family Lamiaceae, Camellia, Camellia, Asteraceae, Aceraceae, and Rutaceae, and a fermentation conversion product obtained by allowing actinomycetes and lactic acid bacteria to act on the extract An antibacterial agent composition comprising 1,3-propanediol and / or 1,2-alkanediol having 5 to 10 carbon atoms is provided.

The antibacterial agent composition of the present invention can be suitably blended into cosmetics. Accordingly, the present invention also provides a cosmetic composition comprising the antimicrobial composition of the present invention. The cosmetic composition of the present invention is provided as any one of skin care cosmetics, makeup cosmetics, toiletry cosmetics, and hair care cosmetics. Specifically, although not limited to these, cosmetics for cleaning, cosmetics for hair, basic cosmetics, makeup cosmetics, aromatic cosmetics, sunscreen / sunscreen cosmetics, nail cosmetics, eyeliner cosmetics, Examples include lip cosmetics, oral cosmetics, and bath cosmetics.
Even when the antibacterial agent composition of the present invention is formulated into a cosmetic composition based on water, it is possible to provide a cosmetic having a uniform and transparent appearance.

  The antibacterial agent composition of the present invention has a high antibacterial effect against bacterial species such as bacteria, mold and yeast that cause contamination of the cosmetic composition, and has a sufficient antiseptic effect when blended into the cosmetic composition. provide. The antibacterial agent composition of the present invention exhibits a uniform and transparent appearance, and can provide a cosmetic having a good appearance. The antibacterial agent composition of the present application can provide a product having a transparent appearance even when blended into water-based cosmetics.

  Fermentation metabolites of the plant extract used in the present invention include plants belonging to the family Lamiaceae, Camelliaaceae, Camellia, Artemisia princes var orientalis, Houttuynia cordata, and mandarin It is a substance obtained by biotransformation by the action of actinomycetes and lactic acid bacteria on extracts from the family plants (Citrus junos). Examples of suitable plants include Scutellaria baicalensis as a plant belonging to the family Lamiaceae, Green tea (Camellia sinensis leaf) as a plant belonging to the camellia family, Artemisia princeps var orientalis, Examples of the plant belonging to the genus Dokudami include Houttuynia cordata, and examples of the citrus family include Citrus junos. Biotransformation uses a culture solution of actinomycetes spp. And lactic acid bacteria (Lactobacillus spp.).

  Each said plant extract can be normally obtained by the various well-known methods of extracting an active ingredient from a plant.

  As an example, when obtaining an Ougon extract, a polar solvent such as ethanol or methanol or a mixed solution of these and purified water is added to the dried Ogon product about 10 times (weight ratio) of the pulverized product, and is extracted with an extractor with a cooling condenser. An extract is obtained by heating at 80 ° C. for 24 hours, and this is filtered and concentrated under reduced pressure to obtain a concentrated extract. Then, after adding purified water corresponding to 2 times (weight ratio) to the concentrated extract obtained, 4 times (weight ratio) of nonpolar solvent such as ethyl acetate and methylene chloride is added and shaken strongly. After that, the upper non-polar solvent layer is separated and concentrated under reduced pressure to obtain the Augon extract.

  As another example, when obtaining an Ougon extract, purified water is added to the dried Ogon product twice (weight ratio), and a polar solvent such as ethanol or methanol or a mixed solution thereof with purified water is added to the pulverized product. A 20-fold weight is added and the extract is obtained by heating at 60 ° C. for 10 hours with an extractor equipped with a cooling condenser to obtain an extract, which is filtered and concentrated under reduced pressure to obtain a concentrated extract. To the resulting concentrated extract, a polar solvent such as ethanol or methanol corresponding to 10 times (weight ratio) or a mixed solution of these and purified water is added and shake-extracted. Then, the Ougon extract is obtained.

  As yet another example, in the case of ougon extract, the main antibacterial active ingredient in ougon extract can be obtained by the following extraction method using supercritical fluid extraction technology. That is, methanol in which 2 to 20 (v / v)% diethylamine or triethylamine is dissolved in 100 parts by volume of carbon dioxide as a main solvent at 70 to 90 ° C. and 4,000 to 6,000 PSI pressure in a supercritical fluid extraction device, Extract 1 ~ 20 parts by volume of one or more alkaline co-solvents selected with ethanol, water or a mixed solvent of these in a reactor, then extract, filter and purify using column chromatography to extract ougon Things are obtained.

  In addition to the above methods, the ougon extract can be obtained by various methods. In addition, a green tea extract, mugwort extract, dokudami extract, and yuzu extract can be obtained by the same extraction method.

  Ougon extract contains baicalein, the main ingredient, baicalin, dimethoxytetrahydroxyflavone (DTF), wogonin, 7-methoxybaicalein, oroxylin-A (oroxylin-A) -A), flavonoids such as skμLlcapflavone I and II, and sterols such as beta-sitosterol and campesterol, sucrose, D-glucose (D -Glucose) and other essential oils and other resins. In particular, specific flavonoids such as baicalin, DTF, or gononin are known to have excellent antibacterial activity. It is known that a trace amount is contained.

  Fermentation conversion products used in the present invention include Actinomycetes spp. And Lactobacillus spp. Isolated from nature of the above extract of green grass, green tea extract, mugwort extract, dokudami extract, yuzu extract. ) To increase the antibacterial activity.

  Fermentation conversion products used in the present invention are, in particular, 20-60 parts by weight derived from ougon extract, 10-30 parts by weight from green tea extract, It is preferable to use a mixture of 30 parts by weight, 10 to 30 parts by weight derived from Artemisia extract, and 10 to 20 parts by weight derived from Yuzu extract. Particularly, a TSB medium containing 10 to 20% of each extract is inoculated with actinomycetes and lactic acid bacteria and cultured for 5 days or 7 days. An example of such a fermented product is MFP (trade name) of Korea Bio Skin Tech.

  The antibacterial agent composition of the present invention is obtained by blending 1,3-propanediol and / or 1,2-alkanediol having 5 to 10 carbon atoms with a fermentation conversion product having high antibacterial properties. The fermentation conversion product exhibits a separated opaque appearance as it is, but a transparent antibacterial agent composition can be obtained by blending 1,3-propanediol and / or 1,2-pentanediol. The antibacterial agent composition of the present invention can also provide a composition having a transparent appearance when blended into a cosmetic composition based on water.

  The blending amount of 1,3-propanediol and / or 1,2-alkanediol having 5 to 10 carbon atoms with respect to the fermentation conversion product contained in the antibacterial agent composition of the present invention is not particularly limited, and is a cosmetic composition. What is necessary is just to select a compounding quantity suitably according to the kind of, use purpose, and a usability | use_condition. Typically, in the case of 1,3-propanediol, it is preferable to use 1/2 to 10 times the amount of the fermentation conversion product. The 1,2-alkanediol having 5 to 10 carbon atoms is particularly preferably 1,2-pentanediol, 1,2-hexanediol, 1,2-octanediol, or 1,2-decanediol. Typically, in the case of 1,2-alkanediol having 5 to 10 carbon atoms, it is preferable to use 1/20 to 20 times the amount of the fermentation conversion product.

  The blending amount of the antibacterial agent composition of the present invention in the cosmetic composition is not particularly limited, and a blending amount exhibiting an antiseptic effect may be appropriately selected according to the type of cosmetic composition and the purpose of use. Typically, the amount of the fermentation conversion product is 0.01% by weight or more, preferably 0.02% by weight or more of the cosmetic composition, and the upper limit is 10% by weight in consideration of the safety of the cosmetic composition, preferably 5% by weight. It is as follows.

  The present invention provides a cosmetic composition containing the antimicrobial composition of the present invention. The cosmetic composition of the present invention is prepared by previously mixing a fermentation conversion product with 1,3-propanediol and / or 1,2-alkanediol having 5 to 10 carbon atoms into the other components of the cosmetic composition. Alternatively, the fermentation conversion product and 1,3-propanediol and / or 1,2-alkanediol having 5 to 10 carbon atoms may be separately added to the other components.

  The cosmetic composition containing the antibacterial agent composition of the present invention includes basic cosmetics such as creams, emulsions and lotions, makeup cosmetics such as lipsticks and foundations, sunscreen / sunscreen cosmetics and hair creams. Examples include hair cosmetics such as hair lotions, cleansing cosmetics, aromatic cosmetics, nail cosmetics, eyeliner cosmetics, lip cosmetics, oral cosmetics, and bath cosmetics.

  The cosmetic composition containing the antibacterial agent composition of the present invention includes various components and additives that are usually used in cosmetic compositions as necessary, for example, inorganic pigments, organic pigments, inorganic powders, organic powders, Hydrocarbons, silicones, esters, triglycerides, waxes, waxes, animal and vegetable oils, surfactants, polyhydric alcohols, sugars, whitening agents, vitamins, amino acids, antioxidants, preservatives, fragrances A thickener, an ultraviolet light inhibitor, an alcohol, a pH adjuster, water and the like may be blended and prepared by a method known per se. The ingredients and additives used in each cosmetic composition are well known to those skilled in the art.

  The pH of the final product as a cosmetic composition is preferably in the range of 4-10. The final pH may be adjusted using a known pH adjuster. In the case of a cosmetic composition based on water, citric acid is preferably used as the pH adjuster. By adjusting the pH with citric acid, it is possible to provide a transparent cosmetic composition in a wide range of pH.

  The cosmetic composition of the present invention thus obtained can be used in the same manner as a normal cosmetic composition, is highly safe against skin, hair, etc., and is excellent in storage stability.

  The present invention will be described in detail below with reference examples and examples, but the present invention is not limited in any way. In addition, all the compounding quantities shown below are weight%.

Reference example 1
Culture of actinomycetes spp. And lactobacilli (Lactobacillus spp.) Isolated from nature for the purpose of enhancing antibacterial activity against ougon extract, green tea extract, mugwort extract, dokudami extract, yuzu extract The liquid was used to obtain a fermentation conversion product. The composition ratio for optimizing the antibacterial properties is as follows: 20-60 parts by weight of ougon extract, 10-30 parts by weight of green tea extract, 10-30 parts by weight of dokudami extract, It was preferable to mix 10-30 parts by weight of the extract and 10-20 parts by weight of the yuzu extract.

As the fermentation conversion product obtained in Reference Example 1, a commercially available MFP (MFP: trade name, manufactured by Korea Bio Skin Tech Co., Ltd.) is particularly preferably used. The fermented product was confirmed for antibacterial properties. As strains to be used, Bacillus subtilis (ATCC21770), Staphylococcus aureus (ATCC29213) as Gram-positive bacteria, Escherichia coli (ATCC25922), Sal.typhimurium (ATCC14028), Pseudomonas aeruginosa (ATCC27853), Staphylococcus serevi858e (ATCC27853), Candida albicans (ATCC32354) was used as the fungus and Aspergillus fumigatus (ATCC90906) was used as the fungus. Aseptically separate 50 μL of sterilized liquid medium into each well in a 96-well plate in a clean bench. First, 100 μL of each test antibacterial sample is divided into wells, and the bacterial concentration is 10 ⁴ cfu / mL ~ Adjust to 10 ⁶ cfu / mL. 50 μL of each well in a 96-well plate was mixed and mixed, and then the bacteria were cultured in a 35 ° C incubator for 24-48 hours, and the mold was cultured at 28 ° C for 72-96 hours to evaluate the minimum growth inhibitory concentration (MIC). did. The results are shown in Table 1.

  As shown in Table 1, the fermentation transformants are Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Streptococcus faecium, Propionibacterium acnes. And antibacterial properties against Staphylococcus epidermidis, Genre streptococcus, Corynebacterium xerosis, and the like.

  In addition, the antimicrobial properties of the MFP obtained in Reference Example 1 did not change even when the pH was changed or added by heating.

The antibacterial activity of MFP and methylparaben was compared using the paper disk agar assay method. Prepare each bacterial solution prepared in sterile saline to 10 ⁸ cfu / mL, sterilize Mueller Hinton agar medium, which is a medium for producing bacterial plate, at 121 ° C for 15 minutes, and then cool to 45-50 ° C to Staphylococcus A mixed bacterial solution of 10 ⁶ cfu / mL of aureus (ATCC29213), Escherichia coli (ATCC25922), Enterococcus spp, Pseudomonas aeruginosa (ATCC27853) is added to the solid medium and used. Place a 6 mm diameter paper disc on Mueller Hinton agar medium, and add 20 μL each of 2.5, 5, 10 wt% aqueous solution of fermentation conversion product and 2.5, 5, 10 wt% ethanol (30%) solution of methylparaben. After each addition and culturing at 37 ° C. for 24 hours, the antibacterial activity was evaluated by comparing the size of the inhibition circle against the growth of the bacteria. The results are shown in FIG. FIG. 1A shows the results for methyl paraben, and FIG. 1B shows the results for MFP. MFP showed stronger antibacterial properties than methylparaben.

Similarly, antibacterial activity of MFP and methylparaben against Pityrosporum spp. (ATCC29213) and Propionibacter acnes (ATCC25922) was compared using a paper disk agar assay method. Prepare each bacterial solution prepared in sterile saline to 10 ⁸ cfu / mL, sterilize Mueller Hinton agar medium, which is a medium for producing bacterial solution plates, at 121 ° C for 15 minutes, and cool to 45-50 ° C to Pityrosporum A mixed bacterial solution of 10 ⁶ cfu / mL of spp. (ATCC29213) and Propionibacter acnes (ATCC25922) is added to the solid medium for use. A 6 mm diameter paper disk was placed on the Mueller Hinton agar broth medium, and 20 μL each of 10% by weight aqueous solution of fermentation conversion product and 10% by weight ethanol (30%) solution of methylparaben were added thereto, and cultured at 37 ° C. for 24 hours Later, the antibacterial activity was evaluated by comparing the size of the inhibition circle for bacterial growth. The results are shown in FIG. Fig. 2A shows the results of antibacterial activity tests against Pityrosporum spp. (ATCC29213, Fig. 2B) Propionibacter acnes (ATCC25922) .In each case, it can be seen that MFP shows stronger antibacterial activity than methylparaben.

Example 1
An aqueous composition was prepared using the MFP obtained in Reference Example 1, and the transparency was observed. The MFP was mixed with various types and amounts of alcohol, and it was confirmed whether or not the appearance of the mixture at room temperature was transparent. Furthermore, the obtained mixture was mixed with purified water at room temperature, and it was visually confirmed whether or not the appearance of the obtained mixture was transparent. The results are shown in Table 2.

表２中、○は外観が透明であることを、×は外観が不透明であることを示す。

In Table 2, o indicates that the appearance is transparent, and x indicates that the appearance is opaque.

  As is apparent from Table 2, the antibacterial agent composition and the aqueous composition having a transparent appearance are obtained only when a certain amount of 1,3-propanediol or 1,2-pentanediol is blended among a plurality of alcohols. It was possible to get.

  The formulation example of this invention cosmetics is described below. In addition, when the above-described storage stability test and safety test were performed on the cosmetic composition of the present invention in these formulation examples, the skin external preparation of the present invention of any formulation example was also antibacterial in the storage stability test, The evaluation of color, odor, and irritation in the safety test was judged as “good”.

＊１ 加水分解卵隔膜水溶液（キューピー社製）
＊２ 酵母エキス（山川貿易社製） Formulation example whitening lotion

* 1 Hydrolyzed egg diaphragm aqueous solution (manufactured by Kewpie)
* 2 Yeast extract (manufactured by Yamakawa Trading Co., Ltd.)

(Manufacturing method) The raw material which belongs to A was heated at 70 degreeC, and it melt | dissolved completely, Then, it cooled to room temperature. Thereafter, raw materials belonging to B were mixed to obtain a whitening lotion (pH: 6.5).

Formulation example

(Manufacturing method) The raw material of the oil phase part belonging to A and the raw material of the water phase part belonging to B were heated to 70 ° C. and completely dissolved, and then the oil phase part was mixed with the aqueous phase part and emulsified with an emulsifier. . The emulsion was cooled to 30 ° C. to obtain an emulsion (pH: 5.0).

＊３ Ｚｅｍｅａ Ｐｒｏｐａｎｅｄｉｏｌ（デュポン社製） Formulation example Moisture cream

* 3 Zemea Propanediol (DuPont)

(Manufacturing method) The raw material of the oil phase part belonging to A and the raw material of the water phase part belonging to B were heated to 70 ° C. and completely dissolved, and then the oil phase part was mixed with the aqueous phase part and emulsified with an emulsifier. . The emulsion was cooled to 30 ° C. to obtain a moisture cream (pH: 7.0).

Formulation example facial cream

(Manufacturing method) After heating the raw material of the oil phase part belonging to A and the raw material of the water phase part belonging to B to 75 ° C. and dissolving, the water phase part was slowly mixed with the oil phase part, and then stirred well, It cooled to 30 degreeC and the face-wash cream (pH: 8.5) was obtained.

Formulation example Sunscreen cream

(Manufacturing method) The raw material of the oil phase part belonging to A and the raw material of the water phase part belonging to B are heated to 75 ° C. and completely dissolved, and then the oil phase part is mixed with the aqueous phase part and emulsified with an emulsifier. . The emulsion was cooled to 30 ° C. to obtain a sunscreen cream (pH: 6.0).

Formulation example Liquid foundation (Shakewell type)

(Manufacturing method) The oil phase part which belongs to A is heated to 60 degreeC, and it cools to room temperature after that. The raw material of the oil phase part belonging to A and the raw material of the water phase part belonging to B were mixed at room temperature and emulsified with an emulsifier to obtain a shake well type liquid foundation.

Formulation example shampoo

(Manufacturing method) The oil phase part which belongs to A was heated to 80 degreeC, and it cooled to room temperature after that, and obtained the shampoo.

Formulation example Conditioner

(Manufacturing method) The oil phase part which belongs to A and B is heated to 80 degreeC, C heated to 80 degreeC is mixed at 80 degreeC, it emulsifies with an emulsifier, and is then cooled to room temperature, and a conditioner is used. Obtained.

Reference example 2
A sun lotion was prepared according to the formulation shown in Table 9 using the MFP of Reference Example 1. The obtained sun lotion was excellent in usability, stability and safety.

  Antibacterial in the antibacterial test method of Reference Example 1 using the obtained sun lotion Staphylococcus aureus (ATCC29213), Escherichia coli (ATCC25922), Pseudomonas aeruginosa (ATCC27853), Candida albicans (ATCC32354) and Aspergillus fumigatus (ATCC90906) It was confirmed. The results are shown in FIG. This sun lotion was confirmed to have sufficient antibacterial properties.

Reference example 3
Using the MFP of Reference Example 1, a moisture cream having the formulation shown in Table 10 was prepared. The resulting moisture cream was excellent in usability, stability and safety.

  The antibacterial properties of the moisture cream were confirmed by the antibacterial test method of Reference Example 1 using Staphylococcus aureus (ATCC29213), Escherichia coli (ATCC25922), Pseudomonas aeruginosa (ATCC27853), Candida albicans (ATCC32354), and Aspergillus fumigatus (ATCC90906). did. The results are shown in FIG. This moisture cream was confirmed to have sufficient antibacterial properties.

Reference example 4
Using the MFP obtained in Reference Example 1, a balancing toner was prepared according to the formulation shown in Table 11. The obtained balancing toner was excellent in usability, stability and safety.

Antibacterial activity was confirmed by the antibacterial activity test method of Reference Example 1 using Staphylococcus aureus (ATCC29213), Escherichia coli (ATCC25922), Pseudomonas aeruginosa (ATCC27853), Candida albicans (ATCC32354), and Aspergillus fumigatus (ATCC90906). The results are shown in FIG. This balancing toner was confirmed to have sufficient antibacterial properties.

The result of the antimicrobial test of methylparaben is shown. The result of the antimicrobial test of MFP is shown. The result of the antibacterial test for Pityrosporum spp. (ATCC29213) of MFP and methylparaben is shown. The result of the antibacterial property test with respect to Propionibacter acnes (ATCC25922) of MFP and methylparaben is shown. The result of the antibacterial property test of the sun lotion of Reference Example 2 is shown. The result of the antimicrobial property test of the moisture cream of Reference Example 3 is shown. The result of the antibacterial property test of the balancing toner of Reference Example 4 is shown.

Claims (6)

  An extract obtained from a plant of the family Lamiaceae, Camellia, Camellia, Asteraceae, Dokudamiaceae, and Citrudaceae, and obtained by allowing actinomycetes and lactic acid bacteria to act on the extract; -An antibacterial agent composition containing propanediol and / or 1,2-alkanediol having 5 to 10 carbon atoms.   The composition of claim 1 further comprising citric acid.   A fermented product obtained by obtaining an extract from a plant of the family Lamiaceae, Camellia, Camellia, Asteraceae, Dokudami, and Rutaceae, and allowing actinomycetes and lactic acid bacteria to act on the extract is trade name MFP The composition according to claim 1 or 2, which is (Bio Skin Tech).   The composition according to any one of claims 1 to 3, comprising 1,3-propanediol in an amount of fermentation conversion product: 1,3-propanediol = 1: 0.5 to 10.   The C5-C10 1,2-alkanediol is contained in an amount of fermentation conversion product: C5-C10 1,2-alkanediol = 1: 0.05-20. A composition according to 1.   A cosmetic composition comprising any one of skin care cosmetics, makeup cosmetics, toiletry cosmetics, and hair care cosmetics, comprising the antibacterial composition according to any one of claims 1 to 5.

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