JP2009197035A - External preparation for skin - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an external preparation for skins, excellent in a moisture-retaining property and the like, although various cosmetics containing the extracts of plants, such as a cosmetic containing an extract of morning-glory, a cosmetic containing the extract of strawberry, and a cosmetic containing an extract of carnation are known. <P>SOLUTION: Provided is the external preparation for the skins, containing an extract of a plant belonging to Myoporaceae, such as Eremophila racemosa, Eremophila nivea, Eremophila maculata, Eremophila glabra, Myoporum parvifolium, Myoporum sandwicense, Myoporum bontioides (Sieb. et Zucc.) A. Gray, or Myoporum boninense Koidz. The external preparation is excellent in a moisture-retaining property and the like, and is useful as a cosmetic or the like. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention relates to a skin external preparation, and more particularly to a skin external preparation that is excellent in moisture retention and is useful as a cosmetic, for example.

Various cosmetics containing plant extracts have been proposed. For example, cosmetics containing a morning glory extract (Patent Document 1), cosmetics containing a strawberry extract (Patent Document 2), cosmetics containing a carnation extract (Patent Document 3), and the like are known.
However, there is no known external skin action for plants belonging to the clamaceae family.

JP 2001-122730 A JP 2001-213750 A JP 2001-220313 A

  An object of the present invention is to provide a skin external preparation that is excellent in moisture retention and the like and is useful as a cosmetic, for example.

  The present invention provides an external preparation for skin comprising an extract of a plant belonging to the clamaceae family.

  ADVANTAGE OF THE INVENTION According to this invention, the skin external preparation excellent in moisture retention etc. can be provided.

  Hereinafter, the present invention will be described in more detail.

  In the genus Crestidae (Myoporaceae in the Clonquist system) used in the present invention, there are the genus Bontia, Eremophila, Myoporum, Oftia, Pholidia, Ranopisoa, Spielmannia, etc., and the species are Eremophila racemosa, Eremophila nivea, Eremophila maculata, Eremophila glabra, Myoporum parvifolium, Myoporum sandwicense, Myoporum bontioides (Sieb. Et Zucc.) A. Gray, Myoporum boninense Koidz.

  Plants belonging to the clam butterfly family (hereinafter referred to as plants of the present invention) can use whole or part of the plant body (for example, leaves, petioles, stems, rhizomes, petals, etc.), preferably leaves, stems, and petals. More preferred are petals, leaves and stems.

A method for preparing an extract from the plant of the present invention will be described.
As a pre-stage for preparing the extract from the plant of the present invention, the plant part (hereinafter referred to as a sample) used for obtaining the extract is preferably dried and pulverized.
The prepared sample is subjected to contact with an extraction solvent.
Examples of usable solvents include water, hot water; aqueous solvents containing lower alcohols having 1 to 4 carbon atoms such as methanol, ethanol, and propanol; lower alkyl esters such as ethyl acetate; ethylene glycol, propylene glycol, Examples include glycols such as glycerin; polar solvents such as acetone and acetic acid; hydrocarbons; known extraction solvents such as nonpolar solvents, preferably aqueous solvents, more preferably water or hot water (generally 50- 100 ° C.). Two or more of these solvents may be combined.

  The extraction can be carried out with or without stirring for a time during which sufficiently soluble components are extracted from the sample. The extraction time is appropriately determined depending on the type or temperature of the extraction solvent, but is typically 30 minutes to 48 hours.

  Specifically, in a preferred embodiment, the dried sample is pulverized, extracted with 10 to 20 volumes of water or hot water, and centrifuged to obtain an extract. Thereafter, filtration is performed, and the filtrate is concentrated under reduced pressure to obtain an extract. The extract may be subjected to a purification operation according to a known method.

  The amount of the extract to be added to the external preparation for skin is, for example, 0.0001 to 10% by mass, preferably 0.001 to 5% by mass.

In the external preparation for skin of the present invention, if necessary, known materials that are usually used as external preparations for skin, such as pigments, fragrances, preservatives, surfactants, pigments, antioxidants, moisturizers, ultraviolet absorbers. Etc. can be appropriately blended.
The external preparation for skin of the present invention can be used in a known form such as cream, milky lotion, lotion, pack and the like.

  EXAMPLES Hereinafter, although an Example demonstrates this invention further, this invention is not limited to the following example.

Example 1
Myoporum bontioides (Sieb. Et Zucc.) A. Gray leaves were dried and ground. To 10 g of the pulverized product, 500 ml of water was added, and extraction was performed by shaking for 120 minutes, followed by centrifugation. The obtained supernatant was concentrated under reduced pressure and then lyophilized. Hereinafter, this is called extract 1.
The stem of Myoporum boninense Koidz. Was dried and pulverized. To 50 g of the pulverized product, 1.5 L of hot water (80 ° C.) was added and subjected to extraction by shaking for 120 minutes, followed by centrifugation. The obtained supernatant was concentrated under reduced pressure and then lyophilized. Hereinafter, this is called extract 2.
Eremophila glabra flowers were dried and crushed. To 50 g of the pulverized product, 1.5 L of water was added, extraction was performed by shaking for 120 minutes, and then a centrifugal separation operation was performed. The obtained supernatant was concentrated under reduced pressure and then lyophilized. Hereinafter, this is referred to as extract 3.
The leaves of Myoporum sandwicense were dried and ground. To 10 g of the pulverized product, 500 ml of water was added, and extraction was performed by shaking for 120 minutes, followed by centrifugation. The obtained supernatant was concentrated under reduced pressure and then lyophilized. Hereinafter, this is referred to as extract 4.

Four types of creams with the following composition were prepared.
70.5% by weight of water
Potassium hydroxide 0.5% by mass
Stearic acid 15% by mass
Butyl stearate 8% by mass
Glycerol monostearate 3% by mass
Extract 1, 2, 3 or 4

  The cream is prepared by adding hyaluronic acid, potassium hydroxide and the extract 1, 2, 3 or 4 to water and stirring and mixing at 70 ° C. to obtain a first mixed solution. Separately, stearic acid, stearin Acid butyl and glyceryl monostearate are heated and melted at 70 ° C. to obtain a second mixed solution, and finally, the first mixed solution and the second mixed solution are mixed.

The moisturizing property of the obtained cosmetic cream was evaluated by a sensory test. The obtained cosmetic cream was used as usual by 10 subjects (female), the moist feeling was scored in 5 stages, and the average score was calculated and evaluated.
5 points: Excellent.
4 points: Excellent.
3 points: Normal.
2 points: Inferior.
1 point: Very inferior.

  As a result, the evaluation score was 4.1 to 4.5 in four types of cosmetic creams. This value was significantly superior to the values (3.2 to 3.4) in which the above experiment was repeated using strawberry leaves, carnation petals, cherry petals, and tulip petals.

  In addition, although the test subject was allowed to use the sample of the above Example for 30 days continuously, the presence or absence of skin abnormality was confirmed, but no abnormality was observed both visually and subjectively.

Next, 24 microliters of the above-mentioned four types of cosmetic creams were applied to a 12 cm ² area of the forearm, and the keratin moisture content was measured before application and 60 minutes after application. SKICON-200 (manufactured by IBI S Co., Ltd.) was used for the amount of horny moisture. When evaluated as a relative value when the amount of keratin moisture before coating was 1, the keratin moisture content relative value for 60 minutes after application of the four types of cosmetic creams was 1.20 to 1.28. This value was significantly superior to values (1.04 to 1.06) in which the above experiment was repeated using strawberry leaves, carnation petals, cherry petals, and tulip petals.

Example 2
As cells producing melanin, cultured B16 melanoma cells derived from mice were cultured in Eagle's MEM medium supplemented with fetal bovine serum to a final concentration of 10%, and the cells were cultured at a concentration of 10 ³ cells / ml. 6 ml is inoculated in each well of the well plate, cultured in a CO ₂ incubator for 5 days, replaced with a medium supplemented with the extract 1, 2, 3 or 4 of Example 1, and further cultured under the same conditions for 3 days. After washing the cells, the cells are peeled off by a scraper treatment, solubilized with sodium dodecyl sulfate (SDS), and the absorbance at 475 nm and 260 nm is measured to obtain S ₄₇₅ and S ₂₆₀ . The melanin inhibition rate was calculated by Equation 1 with the absorbance at 475 nm and 260 nm of cells cultured in a medium not added with the test sample as C ₄₇₅ and C ₂₆₀ . As a positive control, kojic acid was used.

  As a result, the melanin suppression rate of the positive control (addition of 3 mM kojic acid in the medium) was about 56%, and the test sample to which the extract 1, 2, 3 or 4 was added (the extract 1, 2, 3 in the medium) Or 4 was added at 500 μg / ml), and the melanin inhibition rate was about 50 to 54%.

A cream having the following composition was prepared by a conventional method.
Extract 1, 2, 3 or 4 10 parts by weight Glycerol sorbitan fatty acid ester 60 parts by weight Microcrystalline wax 10 parts by weight Olive oil 30 parts by weight Liquid paraffin 180 parts by weight Magnesium stearate 10 parts by weight Propylene glycol 37 parts by weight Magnesium sulfate 7 parts by weight purified water 655 parts by weight

A hair lotion was prepared as follows. (Unit:% by mass)
(1) Extract 1, 2, 3 or 4 2.0
(2) Polyoxyethylene hydrogenated castor oil (50 EO) 0.2
(3) Fragrance 0.1
(4) Ethanol 53.0
(5) Panthenol 0.5
(6) 1,3-butylene glycol 5.0
(7) Purified water remaining (1) After dissolving (2) and (3), add (4) and dissolve uniformly. To this, a mixture of components (5) to (7) was added and stirred until it became uniform to obtain a hair lotion.

A lotion was prepared as follows. (Unit:% by mass)
(1) Extract 1, 2, 3 or 4 5.0
(2) Ethanol 15.0
(3) 1,3-butylene glycol 5.0
(4) Dipotassium glycyrrhizinate 0.5
(5) Fragrance 0.1
(6) Polyoxyethylene hydrogenated castor oil (60 EO) 0.2
(7) Purified water remaining amount (1), (2), (5), (6) is uniformly dissolved, (4) is dissolved (7) is added, (3) is added and mixed uniformly. Dissolved to obtain a lotion.

A skin cream was prepared as follows. (Unit:% by mass)
(1) Extract 1, 2, 3 or 4 1.0
(2) Beeswax 5.0
(3) Cetanol 1.5
(4) Reduced lanolin 8.0
(5) Squalane 29.5
(6) Lipophilic monoglyceride monostearate 5.0
(7) Polyoxyethylene sorbitan monolaurate (20E.O.) 5.0
(8) Propylene glycol 5.0
(9) Purified water remaining amount The oil phase components (1) to (6) were mixed, dissolved, and heated to 75 ° C. On the other hand, the aqueous phase components (7) and (9) were mixed, dissolved, and heated to 75 ° C. Next, the oil phase component was added to the aqueous phase component and pre-emulsified, and then uniformly emulsified with a homomixer. After cooling, (8) was added at 45 ° C. to obtain a skin cream.

  In addition, the result similar to the above was obtained also about the extract (petal, leaf, stem, whole plant) of the plant which belongs to the clamaceae which was illustrated previously except having used in the said Example. The same results as above were obtained even when 40% aqueous ethanol solution and n-hexane were used as the extraction solvent for the extracts 1, 2, 3 or 4.

Claims (1)

  An external preparation for skin comprising an extract of a plant belonging to the family Chlamidae.