JP2009046421A - Skin care preparation for external use - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a skin care preparation for external use, which increases the moisture retaining action of the external preparation mixed with a yeast extract and imparts an excellent feeling of moisture retention to a skin. <P>SOLUTION: The skin care preparation comprises (A) a yeast extract and (B) a liposome containing one or more amino acids selected from the group consisting of tryptophan and histidine. The skin care preparation imparts a feeling of moisture retention to a skin area by its application to the skin area. The feeling of moisture retention is further enhanced by mixing the component (B) with one or more amino acids selected from the group consisting of valine, leucine, isoleucine, threonine, lysine, methionine and phenylalanine. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention relates to an external preparation for skin containing liposomes.

  In order to freshen and moisturize the skin, an external preparation for skin containing a compound having a moisturizing action has been conventionally provided. Yeast extract has high safety and is one of important compounds having a moisturizing action. However, the moisturizing action of the yeast extract is not necessarily high, and it cannot be said that the moisturizing action on the skin by the external preparation for skin containing the yeast extract is sufficient. Examples of the prior art of a skin external preparation containing a yeast extract include packs, creams, and emulsions (see Patent Documents 1, 2, 3, 4 and 5).

  On the other hand, in order to improve the medicinal efficacy of medicinal ingredients, a technique for making medicinal ingredients into liposomes is known, but liposomes containing yeast extract are not known. In addition, about the liposome containing the amino acid which is the compound which concerns on this invention, the technique regarding a skin external preparation is published (for example, refer patent document 6 and 7).

JP 2000-229827 A JP 2002-326922 A JP 2003-160463 A JP 2003-238378 A Japanese Patent Laid-Open No. 2005-220084 Japanese Patent Laid-Open No. 7-285827 JP-A-8-208423

  This invention is made | formed in view of the said situation, The objective is to provide the skin external preparation which improves the moisturizing effect of the skin external preparation mix | blended with a yeast extract, and gives the skin the outstanding moisturizing feeling.

  As a result of intensive studies to solve the above-mentioned problems, the present inventors have found that the above-mentioned problems can be solved by converting the yeast extract into a liposome together with a specific amino acid and containing it in a skin external preparation. The invention has been completed.

That is, the present invention includes the following components (A) and (B):
(A) yeast extract,
(B) An external preparation for skin containing liposomes containing one or two amino acids selected from the group consisting of tryptophan and histidine.

  In the present invention, the component (B) further contains one or two or more amino acids selected from the group consisting of valine, leucine, isoleucine, threonine, lysine, methionine, and phenylalanine, thereby providing a moisturizing action. This is a preferred embodiment because it enhances.

  ADVANTAGE OF THE INVENTION According to this invention, the skin external preparation which gives the skin a moisturizing feeling excellent by including a yeast extract and a specific amino acid in a liposome, and mix | blending with a skin external preparation is obtained. The skin external preparation of the present invention exhibits a further excellent moisturizing effect when used daily.

  Hereinafter, the best mode for carrying out the present invention will be described in detail.

  Although it does not specifically limit as yeast in the yeast extract used in this invention, For example, baker's yeast, beer yeast, wine yeast, sake yeast, etc. can be mentioned. Examples of the yeast extract used in the present invention include an extract with a polar solvent of yeast, a yeast lysed by autolysis, acid hydrolysis, enzymatic degradation, or the like, or the lysate is dried, Those extracted with a polar solvent can be used, but the present invention is not limited to these, and the form of the yeast extract may be a paste or powder from which the solvent has been removed even if it is an extract containing the solvent. It can be used in any form. In addition, as a polar solvent used for extraction, water, ethanol, 1, 3- butylene glycol, propylene glycol, glycerol, or these arbitrary mixed solvents are used, for example.

The yeast extract can be used as a purified extract as it is or after further filtration, concentration and the like. In this invention, what is marketed as a yeast extract can be used. Examples of commercially available products include chito catalyzer (manufactured by Yamakawa Trading Co., Ltd.), Biodynes (manufactured by GSI Creos Co., Ltd.), and the like. The cytocatalyzer is a water-soluble extract obtained by treating, and extracting, concentrating, and non-bromating an active ingredient in baker's yeast by an autolysis method. The blending amount of the yeast extract in the present invention is preferably 7.5 × 10 ^{−3 to} 1.0% by mass in the total amount of the external preparation for skin as a dry pure component.

In the present invention, one or two amino acids selected from the group consisting of tryptophan and histidine are used as essential components. Tryptophan and histidine are allocyclic amino acids (hereinafter also referred to as (different)), both of which are known substances. In the present invention, the amount of one or two amino acids selected from the group consisting of tryptophan and histidine is preferably 7.75 × 10 ^{−7 to} 10.0 mass% in the total amount of the external preparation for skin. In the present invention, in addition to one or two amino acids selected from the group consisting of tryptophan and histidine, an aliphatic amino acid (hereinafter referred to as (fatty)) consisting of valine, leucine, isoleucine, threonine, lysine and methionine. ), An aromatic amino acid composed of phenylalanine (hereinafter also referred to as (good)). The valine, leucine, isoleucine, threonine, lysine, methionine and phenylalanine can be used singly or in combination of two or more selected from these groups, but all are preferably blended. By blending these amino acids, the moisturizing action can be further enhanced. The nine amino acids in the present invention, known as tryptophan, histidine (heterocyclic amino acid), valine, leucine, isoleucine, threonine, lysine, methionine (aliphatic amino acid), and phenylalanine (aromatic amino acid) are known as so-called essential amino acids. ing. In the case of blending one or two or more amino acids selected from the group consisting of valine, leucine, isoleucine, threonine, lysine, methionine and phenylalanine, one or two types selected from the group consisting of tryptophan and histidine It is preferable that it is 7.75 * 10 < ^-7 > -10.0 mass% in the skin external preparation whole quantity as a quantity combined with this amino acid.

  In the present invention, as the amino acid, when the aliphatic amino acid and / or the aromatic amino acid is further added to the heterocylic cyclic amino acid that is an essential component, It is preferable to mix 6 to 10 parts by mass of the aromatic amino acid and 0.5 to 2.0 parts by mass of the aromatic amino acid.

  In the external preparation for skin of the present invention, the yeast extract and the amino acid are contained in the external preparation for skin by inclusion in a liposome containing phospholipid as a constituent component. The moisturizing effect by the yeast extract and the amino acid combination is remarkably exhibited by the formation of liposome.

  The phospholipid used for liposome formation is not particularly limited as long as it is used for cosmetics, quasi drugs, pharmaceuticals and the like. In the present invention, phospholipid is a concept including natural phospholipids, phospholipid derivatives such as hydrogenated phospholipids, synthetic phospholipids, and the like. Examples of phospholipids include natural phospholipids such as phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, and sphingomyelin, hydrogenated phospholipids in which unsaturated carbon chains in natural phospholipids are saturated with hydrogen, and dioleylphosphatidylcholine. Synthetic phospholipids and the like. In the present invention, it is preferable to use phospholipids such as soybean phospholipid and egg yolk phospholipid, hydrogenated soybean phospholipid, hydrogenated phospholipid such as hydrogenated egg yolk phospholipid, and synthetic phospholipid. Hydrogenated soybean phospholipid is particularly preferable. One phospholipid may be used alone, or two or more phospholipids may be used in appropriate combination.

  Stabilizers such as cholesterol, glucose, higher alcohols, nonionic surfactants, ionic surfactants and the like can be added for the purpose of stabilizing liposomes. The liposome stabilizer can be mixed with a phospholipid or the like during the preparation of the liposome, for example, to be a constituent component of the liposome.

  Examples of the nonionic surfactant include sorbitan fatty acid esters (such as sorbitan monostearate and sorbitan sesquioleate), glycerin fatty acids (such as glyceryl monostearate), and propylene glycol fatty acid esters (propylene glycol monostearate). ), Hydrogenated castor oil derivative, glycerin alkyl ether, polyoxyethylene (hereinafter referred to as POE) sorbitan fatty acid esters (POE sorbitan monooleate, POE sorbitan monostearate, etc.), POE sorbite fatty acid esters (POE sorbite mono) Laurate, etc.), POE glycerin fatty acid esters (POE glycerin monoisostearate, etc.), polyethylene glycol (hereinafter referred to as PEG) fatty acid esters (PEG monoo Ate, PEG distearate, etc.), POE alkyl ethers (POE2-octyldodecyl ether, etc.), POE alkylphenyl ethers (POE nonylphenyl ether, etc.), pluronic types, POE polyoxypropylene (hereinafter referred to as POP) alkyl. Examples include ethers (POE / POP2-decyltetradecyl ether), tetronics, POE castor oil / hardened castor oil derivatives (POE castor oil, POE hardened castor oil, etc.), sucrose fatty acid esters, alkyl glucosides, and the like. it can. One or more nonionic surfactants are arbitrarily selected and blended.

  The preparation of the liposome containing the yeast extract and the amino acid is not particularly limited and may be prepared by a known method such as an ultrasonic method, a homomixer method, a thin film method, an injection method, or a surfactant removal method. it can. For example, if necessary, a stabilizer such as polyhydric alcohol or cholesterol is heated and mixed with phospholipid and dissolved. Thereafter, the yeast extract and the amino acid are added, for example, in the form of a yeast extract solution and an amino acid solution, mixed with stirring, added with water, and further mixed with stirring. Thereafter, it is treated with a high-pressure mixer (for example, a high-pressure emulsifier such as a microfluidizer or an APV homogenizer) to form liposomes. Next, when warmed, the solution is cooled to room temperature to obtain a liposome solution. In addition, when the mixture to be processed by the high-pressure mixer is a dispersion, it is preferable to increase the dispersion by high-speed stirring (for example, stirring speed of 3000 rpm or more with a homomixer) in the step before the processing.

  Further, in the production method exemplified above, a liposome from which a yeast extract and / or an amino acid is removed with a component such as phospholipid is prepared, and then the liposome and the removed component (yeast extract and / or amino acid) are necessary. If present, other inclusion components can be further mixed and stirred, preferably at high speed, and the like, and the like. Furthermore, a yeast extract containing a sufficient amount of yeast extract and / or amino acid, or a liposome containing no yeast extract and / or amino acid, and then preparing the required amount It is also possible to prepare the mixture in the same manner as described above by mixing amino acids and the like. In preparing liposomes containing yeast extract and amino acids by these methods, for example, commercially available yeast extract and liposomes not containing amino acids can be used. A stable yeast extract and a liposome containing a high concentration of amino acids can be efficiently obtained.

  Examples of the commercially available yeast extract and liposomes not containing amino acids include NIKKOL Aquasome BH and LA (manufactured by Nikko Chemicals). In the present invention, these commercially available products may be used as they are, but it is also an efficient method to convert them into specifications that are easy to use in preparing the external preparation for skin of the present invention, such as increasing the concentration of liposomes.

  Liposomes are spherical vesicles consisting of a single layer or multiple layers of a phospholipid bilayer, and the yeast extract and / or amino acids are incorporated (included) in the phospholipid membrane or endoplasmic reticulum. . The particle diameter of the liposome is preferably an average particle diameter of 30 to 200 nm. Among these, 40 to 100 nm is more preferable. When the average particle size exceeds 200 nm, the penetration into the skin is inferior, which is not preferable. In addition, the particle diameter of the liposome was measured at room temperature using a concentrated particle size analyzer FPAR-1000 (manufactured by Otsuka Electronics Co., Ltd., light scattering photometer).

  Liposomes are generally present as aqueous dispersions and are optionally diluted in water. In the skin external preparation, it is optionally diluted with water in the skin external preparation. The liposome in the present invention is a concept including an optionally diluted liposome aqueous dispersion present in the skin external preparation.

In the present invention, the yeast extract and the amino acid are included in the liposome and contained in the external preparation for skin. However, the liposome is only a liposome containing a mixture of the yeast extract and the amino acid. The predetermined yeast extract and the amino acid may be present in the liposome in the external preparation for skin. For example, each of the yeast extract and the liposome separately contained in the amino acid may be contained in the external preparation for skin. Further, in the case of using a mixture as an amino acid, not only liposomes containing mixed amino acids but also liposomes containing one kind of amino acid or any kind of mixed amino acid are included in the external preparation for skin. It doesn't matter. In addition, also about a yeast extract, when using 2 or more types, it is the same as the case of an amino acid. In the present invention, the expressions of claims 1 and 2 are taken as including all the embodiments of these liposomes. That is, for example, claim 1 includes the following component (A):
(A) Liposomes containing yeast extract and the following component (B):
(B) A skin external preparation containing liposomes containing one or two amino acids selected from the group consisting of tryptophan and histidine is included. In the present invention, a liposome containing a mixture of a yeast extract and the amino acid is preferable in terms of a moisturizing effect.

  When the liposome containing the yeast extract and amino acid is contained in the external preparation for skin, the yeast extract and amino acid are not included, or the liposome does not include a predetermined amount of the yeast extract and amino acid, and the yeast. An extract and an amino acid may be blended as one component of a skin external preparation formulation, and a liposome containing a predetermined amount of the yeast extract and amino acid may be formed when preparing the skin external preparation.

  In the external preparation for skin of the present invention, in addition to the above-mentioned components, other components that are usually used in external preparations for skin such as cosmetics, quasi-drugs, and pharmaceuticals can be appropriately blended within a range that does not impair the effects of the present invention. it can. As the optional compounding component, a substance contained in the component may be a plurality of components, and includes a description partially overlapping with the above, for example, oil, surfactant, humectant, Polyhydric alcohol, thickener, water-soluble polymer, film forming agent, water-insoluble polymer, oil gelling agent, polymer emulsion, powder, pigment, dye, lake, lower alcohol, saccharide, UV absorber, Non-amino acids, vitamins, whitening agents, skin activators, blood circulation promoters, antiseborrheic agents, anti-inflammatory agents (anti-inflammatory agents), plant extracts, organic acids, organic amines, sequestering agents, A pH adjuster, antioxidant, antibacterial agent (preservative antiseptic), astringent, refreshing agent, fragrance, water and the like can be mentioned.

  Of the optional ingredients, specific examples of the oil include oils such as hydrogenated oil, owl, cacao butter, hydrogenated castor oil, olive oil, castor oil, macadamia nut oil, evening primrose oil; beeswax, lanolin, gay wax, Candelilla wax, carnauba wax, ibota wax, jojoba wax, jojoba oil, liquid lanolin and other waxes; solid paraffin, ceresin, microcrystalline wax, polyethylene wax, liquid paraffin, squalane, polybutene, volatile hydrocarbons and other hydrocarbon oils; Cetyl acid, isopropyl myristate, isopropyl palmitate, cetyl 2-ethylhexanoate, octyldodecyl myristate, octyl stearate, octyl palmitate, diisopropyl adipate, di-2-ethylhexyl sebacate, dica Neopentyl glycol phosphate, glyceryl trimyristate, glyceryl tri-2-ethylhexanoate, glyceryl trioctanoate, glyceryl triisostearate, diglyceryl diisostearate, diglyceryl triisostearate, pentaerythritol tetra-2-ethylhexanoate, Ester oils such as pentaerythritol dioctanoate, trimethylolpropane tri-2-ethylhexanoate, trimethylolpropane triisostearate, diisostearyl malate; stearic acid, palmitic acid, myristic acid, 12-hydroxystearic acid, behe Higher fatty acids such as acid, isostearic acid and oleic acid; cetyl alcohol, stearyl alcohol, cetostearyl alcohol, behenyl alcohol, isostearyl alcohol Higher alcohols such as oleyl alcohol and octyldodecanol, chain polysiloxanes such as dimethylpolysiloxane, methylphenylpolysiloxane and diphenylpolysiloxane, cyclic such as octamethylcyclotetrasiloxane, decamethylcyclopentasiloxane and dodecamethylcyclohexasiloxane Examples thereof include modified polysiloxanes such as silicone, amino-modified polysiloxane, alkyl-modified polysiloxane, and fluorine-modified polysiloxane. One or more oil components can be arbitrarily selected and blended.

  Among the optional blending components, specific examples of the polyhydric alcohol include, for example, glycerin, 1,3-butylene glycol (1,3-BG), 1,2-pentanediol, erythritol, sorbitol, xylitol, multi Tall, propylene glycol, dipropylene glycol (DPG), diglycerin and the like. One or more polyhydric alcohols are arbitrarily selected and blended.

  Specific examples of the humectant among the optional ingredients include lactate (sodium, etc.), sodium pyrrolidonecarboxylate (PCA soda), urea, and the like. One or more moisturizers are arbitrarily selected and blended.

  Among the optional components, specific examples of the water-soluble polymer include, for example, guar gum, quince seed, carrageenan, galactan, gum arabic, pectin, mannan, starch, pullulan, xanthan gum, curdlan, methylcellulose, hydroxyethylcellulose. Carboxymethyl cellulose, methyl hydroxypropyl cellulose, chondroitin sulfate, dermatan sulfate, glycogen, heparan sulfate, hyaluronic acid, sodium hyaluronate, tragacanth gum, keratan sulfate, chondroitin, mucoitin sulfate, hydroxyethyl guar gum, carboxymethyl guar gum, dextran, kerato sulfate, Locust bean gum, succinoglucan, caronic acid, chitin, chitosan, carboxymethyl chitin, agar, polyvinyl alcohol Lumpur, polyvinyl pyrrolidone, carboxyvinyl polymer, sodium polyacrylate, PEG, and the like. One or more water-soluble polymers can be arbitrarily selected and blended.

  The external preparation for skin of the present invention can be prepared according to a conventional method by blending the above components. Further, as described above, when preparing the skin external preparation, liposomes may be formed simultaneously with the preparation of the skin external preparation.

  Although it does not specifically limit as a form of this invention skin external preparation, For example, an emulsion, cream, a lotion, a pack, etc. are mentioned. Further, the dosage form is not particularly limited, and examples thereof include gels, dispersions, ointments, liquids for external use, creams, and the like. In particular, the gel type is preferable from the viewpoint of the moisturizing effect.

  Hereinafter, the present invention will be specifically described with reference to examples. The compounding amount is mass% unless otherwise specified. Prior to the description of the examples, the effect test method used in the present invention will be described.

Moisturizing effect An effect test was conducted by 10 female panelists with experience in sensory evaluation of cosmetic use. The panelist applied an appropriate amount of the test product to the face after washing the face, and sensoryly evaluated the moisturizing feeling 2 hours after the application.

(Evaluation criteria)
A: Strongly moisturized.
○: Moisturizing is felt.
Δ: Slight moisture retention.
X: Moisture retention is not felt.

[Preparation of amino acid solution]
The components listed in Table 1 were mixed to prepare amino acid solutions AD.

[Preparation of yeast extract solution]
A yeast extract liquid A (1.5% by mass as a dry pure yeast extract) comprising the components listed in Table 2 was prepared. For the preparation, chitocatalyzer BG30 (manufactured by Yamakawa Trading Co., Ltd.) was used.

[Preparation of liposomes]
Liposomes A to G were produced by the following method using the components listed in Table 3. In Table 3, the ratio of each component used is shown in mass% (total blending amount 100 mass%). The amino acid solutions A to D are those shown in Table 1, and the yeast extract solution A is that shown in Table 2. A part of hydrogenated soybean phospholipid, glycerin and DPG was mixed at 50 ° C., and then the remainder of DPG and cholesterol were added and dissolved by heating at 50 ° C. Thereafter, the yeast extract liquid A and the corresponding amino acid liquid were added and mixed with stirring. Then, purified water was added to make 100% by mass and mixed with stirring. Finally, it was processed with a high-pressure emulsifier (“Microfluidizer” manufactured by Microfluidics, USA) to obtain liposomes. The average particle diameter of the obtained liposome was 45 nm. Table 3 lists the components of non-liposomes A and B, which are non-liposome compositions in which yeast extract liquid A and amino acid liquid A or B are blended. This non-liposome was prepared by mixing each component.

(Note) NIKKOL Resinol S-10 (Nikko Chemicals)

[Preparation of external preparation for skin]
[Examples 1 to 4, Comparative Examples 1 to 5]
A predetermined amount of ingredients shown in Table 4 were added to a skin external preparation (gel cream) to obtain a gel preparation. Liposomes A to G and non-liposomes A and B are those shown in Table 3.

  An effect test was conducted on the external preparations for skin (gel cream) of Examples 1 to 4 and Comparative Examples 1 to 5, and the evaluation results are shown in Table 5.

  As can be seen from Table 5, the skin external preparation containing the yeast extract and the liposome containing the cyclic amino acids tryptophan and / or histidine is the skin external preparation containing the liposome containing only the yeast extract. Preparation (Comparative Example 1), external preparation for skin containing liposomes containing only tryptophan or histidine (Comparative Examples 2 and 3) and external preparation for skin containing non-liposomal product of yeast extract and tryptophan or histidine (Comparative Example) 4 and 5), it can be seen that the skin is synergistically excellent in moisturizing feeling. Further, an external preparation for skin which further contains an aliphatic amino acid composed of valine, leucine, isoleucine, threonine, lysine and methionine, and an aromatic amino acid composed of phenylalanine in a heterocyclic amino acid composed of tryptophan and / or histidine as amino acids (Examples) 4) shows that the moisturizing feeling on the skin is further enhanced.

[Examples 5 to 8]
Hereinafter, the Example (Examples 5-8 (gel cream)) of the external preparation for skin which consists of a composition of Table 6 of this invention is shown. In addition, manufacture was performed according to the method of Examples 1-4. Liposomes H to K in Table 6 were prepared as shown in Table 7 prepared in the same manner as in Table 3. In addition, the amino acid solutions A to D in Table 7 are those shown in Table 1, and the yeast extract solution A is shown in Table 2.

(Note) NIKKOL Resinol S-10 (Nikko Chemicals)

[Examples 9 to 17]
Hereinafter, the Example (Examples 9-17 (gel cream)) of the external preparation for skin which consists of a composition of Table 8 of this invention is shown. In addition, manufacture was performed according to the method of Examples 1-4. The liposomes A, B, and L to S included in Table 8 were those shown in Table 3 and those shown in Table 9 prepared in the same manner. The amino acid solutions E to L in Table 9 are those shown in Table 10, and the yeast extract solution A is that shown in Table 2.

(Note) NIKKOL Resinol S-10 (Nikko Chemicals)

[Examples 18 to 20]
Hereinafter, the external preparation for skin of the present invention will be shown. In addition, the used amino acid and yeast extract liquid were prepared as follows.

[Preparation of amino acids]
The components listed in Table 11 were mixed to prepare amino acids M and N.

[Preparation of yeast extract solution]
Ingredients listed in Table 12 were mixed to give a yeast extract dry pure content of 0.4% by mass (B), 2.0% by mass (C), 4.0% by mass (D) of yeast extract B to D was prepared. In addition, these yeast extract liquids were prepared using cytocatalyzer BG30 (made by Yamakawa Trading Co., Ltd.).

Example 18 Skin lotion component Amount (% by mass)
(1) 1,3-BG 5.0
(2) DPG 2.0
(3) Phenoxyethanol 0.2
(4) Purified water remaining amount (5) Hydrogenated soybean phospholipid 1.0
(6) 1,3-BG 8.0
(7) Cholesterol 0.5
(8) Amino acid N 0.1
(9) Yeast extract liquid B 25.0
(10) Purified water 25.0
Total 100.0

(Manufacturing method)
The mixed solution of component (5)-(10) was processed with the high-pressure emulsifier at 50 degreeC, and it cooled to 30 degreeC, and prepared the liposome which included the amino acid and the yeast extract. Components (1) to (4) were dissolved by stirring, the liposomes were added, and the mixture was stirred and mixed to obtain a skin lotion.

Example 19 Lotion (1) 1,3-BG 5.0
(2) DPG 2.0
(3) Phenoxyethanol 0.2
(4) Purified water remaining amount (5) Hydrogenated soybean phospholipid 1.0
(6) 1,3-BG 8.0
(7) Cholesterol 0.5
(8) Amino acid M 10.0
(9) Yeast extract liquid C 25.0
(10) Purified water 24.0
Total 100.0

(Manufacturing method)
The mixed solution of component (5)-(10) was processed with the high-pressure emulsifier at 50 degreeC, and it cooled to 30 degreeC, and prepared the liposome which included the amino acid and the yeast extract. Components (1) to (4) were dissolved by stirring, the liposomes were added, and the mixture was stirred and mixed to obtain a skin lotion.

Example 20 Cream component Blending amount (% by mass)
(1) Liquid paraffin 5.0
(2) 2-ethylhexyl stearate 15.0
(3) Vaseline 3.0
(4) Cetyl alcohol 1.0
(5) Polyoxyethylene (20) sorbitan monostearate 1.0
(6) Polyoxyethylene (20) oleyl ether 2.5
(7) DPG 5.0
(8) Phenoxyethanol 0.2
(9) Purified water remaining amount (10) Hydrogenated soybean phospholipid 1.0
(11) 1,3-BG 8.0
(12) Cholesterol 0.5
(13) Amino acid N 10.0
(14) Yeast extract liquid D 25.0
(15) Purified water 15.0
Total 100.0

(Manufacturing method)
The mixed solution of components (10) to (15) was treated with a high-pressure emulsifier at 50 ° C. and cooled to 30 ° C. to prepare liposomes containing amino acids and yeast extract. Components (1) to (9) were heated and mixed and dissolved at 80 ° C, emulsified, cooled to 30 ° C, the liposomes were added, and the mixture was stirred and mixed to obtain a cream.

[Examples 21 to 22]
Hereinafter, the external preparation for skin of the present invention will be shown.

Example 21 Gel cream component Amount (% by mass)
Liposomes (Note 1) 23.6
Amino acid M 1.0
Yeast extract liquid C 25.0
Glycerin 7.0
1,3-BG 5.0
DPG 3.0
Phenoxyethanol 0.5
Carboxyvinyl polymer 0.8
Methylpolysiloxane 3.0
Pyridoxine dicaprate 0.1
Ascorbyl tetra-2-hexyldecanoate 0.2
Allantoin 0.1
Potassium hydroxide 0.3
Purified water residue
Total 100.0

(Note 1) Liposomes prepared by mixing the components shown in Table 13 below, stirring at 50-60 ° C at high speed, and cooling to 30 ° C.

(Note 2) NIKKOL Resinol S-10 (Nikko Chemicals)

[Production method]
The liposome (Note 1) was mixed with the amino acid M and the yeast extract liquid C and stirred at 50 to 60 ° C. at high speed to encapsulate the amino acid and the yeast extract in the liposome (Note 1). On the other hand, carboxyvinyl polymer was added and dispersed in purified water, and potassium hydroxide was added and neutralized to form a gel. Next, the remaining components were added to the gel, and liposomes encapsulating the amino acid and yeast extract were added and stirred to prepare a gel cream.

Example 22 Gel cream component content (mass%)
Liposomes (Note 1) 27.1
Amino acid N 0.1
Yeast extract B 25.0
POE (60) hydrogenated castor oil 0.1
Glycerin 3.0
1,3-BG 7.0
Phenoxyethanol 0.5
Carboxyvinyl polymer 0.7
Ascorbyl tetra-2-hexyldecanoate 0.2
Caustic potash 0.3
Allantoin 0.1
Purified water residue
Total 100.0

(Note 1) NIKKOL Aquasome BH Conch (Nikko Chemicals)

[Production method]
A gel cream was prepared in the same manner as in Example 21.

  A topical skin preparation containing a yeast extract and a liposome containing a specific amino acid gives a synergistic moisturizing effect to the skin, and is applied to various fields such as cosmetics and pharmaceuticals including quasi-drugs. .

Claims (2)

The following components (A) and (B):
(A) yeast extract,
(B) A skin external preparation containing liposomes containing one or two amino acids selected from the group consisting of tryptophan and histidine.   The skin external preparation according to claim 1, wherein the component (B) further contains one or two or more amino acids selected from the group consisting of valine, leucine, isoleucine, threonine, lysine, methionine and phenylalanine.