JP2008074844A - Vitamin c effect-enhancing composition - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To search concrete requirement and optimization of an antioxidant composition bringing about overcoming of these defect and pharmacodynamic effects in order to solve problems in which a conventional provitamin C-containing composition was not excellent in pharmacodynamic effects for immediately retaining vitamin C concentration in human body in a high level. <P>SOLUTION: A vitamin C effect-enhancing composition comprises one or more kinds of materials selected from a group consisting of fruit and leaf of Lycium chinense and processed products thereof as a source for supplying provitamin C, and one or more kinds of materials selected from the group consisting of an enzyme for converting provitamin C to vitamin C and/or a material containing the enzyme. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to compositions containing provitamin C, i.e. vitamin C derivatives and / or vitamin C precursors, rather than vitamin C, i.e. L-ascorbic acid itself, in particular converting provitamin C to vitamin C The present invention relates to a composition (enhancing vitamin C effect composition) containing an enzyme to be used. Such a vitamin C efficacy enhancing composition can be used, for example, for health supplements, pharmaceuticals, cosmetics, feed addition for livestock / pets.

  Vitamin C, an antioxidant / reducing substance such as ascorbic acid or its derivatives / precursors / analogues, is used for pharmaceuticals, cosmetics, food, feed for livestock and aquatic animals, pet animal diet, cells or culture medium for culture of microorganisms It is widely used as an industrial reducing agent and antioxidant for films, photographic paper, printing paper, etc. Recently, reducing substances such as ascorbic acid or derivatives thereof are effective to prevent spots, freckles, etc. caused by sunburn, and derivatives of ascorbic acid or derivatives thereof are used in quasi drugs and cosmetics. .

  Since ordinary L-ascorbic acid becomes unstable and oxidatively decomposes when it is in an aqueous solution, it has been devised to be chemically modified to derivatives that are difficult to oxidatively decompose. Accordingly, the conversion efficiency to vitamin C is poor, and for this reason, a fatal defect that the vitamin C effect is difficult to be exhibited was inherent. On the other hand, vitamin C derivatives that tend to exert vitamin C effects do not protect the 2,3-enediol part of the vitamin C molecule, and as a result, are easily oxidatively decomposed and easily browned and oxidatively decomposed. Since the preparation was unstable, it was difficult to withstand the practical use as it was. Among conventional L-ascorbic acid derivatives, the stability is not sufficient, especially in aqueous solution, and irreversible hydrolysis, precipitation, and coloring to diketogulonic acid due to thermal stress and oxidation. There were various problems such as changes in viscosity when used in combination with odors and thickeners.

  Ascorbic acid, a vitamin C derivative, is converted to provitamin C, that is, a vitamin C derivative, to vitamin C itself after administration or after addition in order to exert vitamin C efficacy by administration or addition to a living body, body part or body component. It is necessary that this conversion action is performed by an enzyme originally contained in a living body, a biological part or a biological component that has been administered or added, particularly an enzyme that converts to vitamin C depending on the type of provitamin C. It was a passive vitamin C conversion.

  In addition, the applicant of the present application inside the human body (1) to increase the concentration of vitamin C to a high level, (2) to have a rapid effect on increasing the concentration of vitamin C, (3) to rebound the high level of vitamin C Patent Document 1 (Japanese Patent Application Laid-Open No. 2007-23008) discloses a novel antioxidant-containing composition in order to keep it continuously.

In Patent Document 2 (Japanese Patent Laid-Open No. 2005-194255), vitamin C derivatives are used, and the activity is extremely high and the activity is enhanced. For internal use and / or injection for prevention and treatment of malignant tumors A composition is disclosed.
JP 2007-23008 A JP 2005-194255 A

  The present invention has been made in view of such circumstances, and enhances vitamin C efficacy by appropriately controlling the rate and conversion rate at which provitamin C administered and / or added to a living body is converted to vitamin C. An object is to provide a composition.

  In addition, conventional provitamin C-containing compositions, regardless of whether they are commercial products or research and development, treat normal provitamin C as a simple substance, and contain other enzymes that convert provitamin C to vitamin C. Therefore, the initial rate of rise in blood vitamin C concentration and tissue vitamin C concentration after ingestion of provitamin C, the degree of achievement and sustainability of high concentration level, and the stability of vitamin C after absorption in the body Although it was inferior in terms of distribution range and deep tissue penetration, as a result, it did not excel in the medicinal properties that quickly and continuously maintain the vitamin C concentration inside the human body. It is an object of the present invention to search for the constituent requirements and optimization of a specific antioxidant composition.

  As a result of diligent research, the present inventors have found that a vitamin C efficacy-enhancing composition containing provitamin C and a specific converting enzyme that appropriately controls the conversion rate and conversion rate of provitamin C to vitamin C. The present inventors have found that the conversion rate and conversion rate of vitamin C converted in the living body can be dramatically improved, and have completed the present invention.

  That is, in order to solve the above-mentioned problems, the present invention provides one or more kinds selected from the group consisting of wolfberry seeds, bamboo leaves, and processed products as provitamin C sources, and Vitamin C efficacy-enhancing composition containing one or more selected from the group consisting of an enzyme that converts provitamin C to vitamin C and / or the enzyme-containing material (that is, the efficacy of vitamin C was enhanced) Provitamin C-containing composition).

  In the above-mentioned vitamin C effect-enhancing composition, it is desirable that the processed product of wolfberry as a source of provitamin C is a dried powder of coconut and / or a water-soluble extract.

  In addition, the content of the enzyme that converts provitamin C to vitamin C is preferably one or more selected from the group consisting of almond fruit, the processed product, the dry powder, and the water-soluble extract. .

  It is desirable that the enzyme that converts provitamin C to vitamin C is beta-glucosidase.

  In addition, the dried powder of persimmon as a source of provitamin C and / or fine particles coated with the water-soluble extract with a biopolymer and / or an enteric polymer, and almonds containing a beta-glucosidase Desirably, the dried powder / water-soluble extract of fruit and / or edible meal is composed of fine particles coated with a biopolymer and / or an enteric polymer.

  Moreover, as a provitamin C supply source, dried coconut powder and / or the water-soluble extract thereof are coated with a biopolymer and / or an enteric polymer, and are obtained as a beta-glucosidase-containing substance. Almond fruit and / or edible rice cake dry powder / water-soluble extract containing almond fruit-derived fine particles coated with biopolymer and / or enteric polymer Ascorbic acid-2-O-beta-glucoside 1 millimole (about 338 milligrams) contains 20-500 units (international unit) of the enzyme active ingredient of beta-glucosidase contained in almond seed-derived microparticles It is desirable.

  The biopolymer is preferably one or more selected from the group consisting of collagen, gelatin, chitin, chitosan, polylactic acid, and poly-L-lysine.

  The efficacy enhancing composition of the present invention is mainly composed of a provitamin C-containing composition, and as a mixed component, a composition containing an enzyme that converts provitamin C to vitamin C has a combination combination and a specific mixture ratio. It is a composition that has been carefully devised, and furthermore, to the mixed composition, a specific kind of antioxidant that has been found to be compatible with these, and / or a natural origin that has also been found to be compatible It is a composition containing a polymer. These antioxidant-containing compositions of the present invention have vitamin C and / or provitamin C efficiently distributed in the body from the blood, penetrate into the deep part of each tissue, and are converted from provitamin C and provitamin C. Makes it possible to quickly and rapidly maintain vitamin C concentrations in the blood and deep in each tissue.

  The present invention is a composition for enhancing vitamin C efficacy, and specific vitamin C efficacy includes all the efficacy of conventional L-ascorbic acid or a derivative-containing composition, and at the same time, further enhances action sustainability and efficacy enhancement. For example, it can be used in health supplements, pharmaceuticals, quasi drugs, cosmetics, detergents, foods, feeds. That is, as a use of such a vitamin C efficacy enhancing composition, it can be used for a use in which L-ascorbic acid or a derivative thereof or a salt thereof is generally used, and in particular, a sustained-type antioxidant, vitamin C enhancer, Examples include anti-prophylactic agents, whitening agents, anti-pigmentation treatments, melanin-suppressing and reducing agents, free radical (active oxygen) disease prevention and treatment agents, allergic disease prevention and treatment agents, and wound healing agents. Exhibits excellent effects.

  The scope of application of the antioxidant-containing composition of the present invention and the target diseases are as follows: Provitamin C in the composition of the present invention is transformed into ascorbic acid after infiltrating into blood, tissues, or skin, and damaged blood vessel walls and tissues. Promotes collagen and elastin synthesis in skin and subcutaneous tissue, promotes cell growth and tissue regeneration, superoxide, hydroxyl radical, hydrogen peroxide, singlet oxygen, nitric oxide, hydroxyperoxide, lipid hydroperoxide, monoxide Scavenging active oxygen such as nitrogen and peroxynitrite is effective for various inflammations, tissue damage, and various lifestyle-related diseases. The composition of the present invention is effective for melanin suppression, UV injury protection, and wrinkle / tarmi protection through the medicinal effects of provitamin C.

  The active oxygen / free radical that enhances vitamin C efficacy by the vitamin C efficacy enhancing composition of the present invention and can be eliminated by vitamin C as a result is a molecule containing an unpaired electron and a molecule containing a highly reactive oxygen. Or an atom, in particular including superoxide anion radical, hydroxyl radical, hydrogen peroxide, singlet oxygen, nitric oxide, hydroxyperoxide and the like.

  The cocoon used in the present invention refers to a leaf of Lycium barbarum, a wolfberry (Lycium chinense), a cocoon (Solanaceae), a coconut, a wolfberry (Lycine chinense Mill), and the like. It is usually available as a cultivated plant, raw material for medicinal herbs.

Provitamin C is a generic term for what is commonly called various names such as vitamin C derivatives, vitamin C precursors, vitamin C analogs, ascorbic acid derivatives, ascorbic acid precursors, ascorbic acid analogs, etc. It is shown. Provitamin C in the present invention mainly refers to ascorbic acid-glucoside. Conventionally speaking, ascorbic acid-glucoside, ascorbic acid-2-glucoside is enough to be understood without ascorbic acid-2-O-alpha-glucoside. Although synonymous with the present invention, ascorbic acid-2-O-beta-glucoside (ascorbic
acid-2-O-beta-glucoside).

  Ascorbic acid-2-O-beta-glucoside is contained in dried powder prepared by crushing wolfberry, especially its fruit. Alternatively, it can be prepared as a water-soluble component fractionated from crushed fruit of wolfberry. Ascorbic acid-2-O-beta-glucoside is identified by HPLC (High Performance Liquid Chromatography) to separate water-soluble components, retention time of pure substances, 1: 1 mol of ascorbic acid and glucose due to beta-glucosidase action Can be verified by generating ratios.

  The enzyme that converts provitamin C to vitamin C differs in the type of the enzyme according to the type of provitamin C. In the present invention, however, the beta that converts ascorbic acid-2-O-beta-glucoside into ascorbic acid is used. -Mainly refers to beta-glucosidase.

  Almonds as a beta-glucosidase source are widely available as edible materials, but unheated materials are preferred. Almond fruits can be finely crushed with a French press or a vortex mixer and used as a dry powder. It can be prepared as a water-soluble component fractionated from crushed almond fruits. In particular, the composition for enhancing vitamin C efficacy according to the present invention comprises an ascorbic acid-2-O-beta-glucoside-containing component (such as wolfberry) and an enzyme (beta-glucosidase) -containing component that converts provitamin C to vitamin C. In the form of coexistence of two single active ingredients of wolfberry, when wolfberry fruit extract is selected as the component containing ascorbic acid-2-O-beta-glucoside, An almond extract with many impurities is suitable as an enzyme (beta glucosidase) -containing component to be converted to vitamin C in the combination of two single agents with respect to the actual extract.

  In addition, as a beta-glucosidase supply source, edible rice cakes such as red rice cake, white rice cake and black rice cake, and edible yeast, acetic acid bacteria and lactic acid bacteria can be used. In particular, microorganisms used for food such as beer, wine, liquor, vinegar, yogurt, and kale are preferable. These are preferably the cells themselves excluding the culture solution, and are preferably disrupted cells. However, it can also be prepared as a water-soluble component fractionated from crushed cells.

  Advantageously, ascorbic acid-2-O-beta-glucoside-containing components such as wolfberry seed dry powder and / or water-soluble extract in the present invention do not come into contact with beta-glucosidase from the beginning. For this reason, it is advantageous to shape the processed product of the wolfberry as fine particles coated with a living body-derived polymer and / or an enteric polymer. The addition amount of the processed product of the cocoon in the fine particles is preferably 1 to 50% by weight, more preferably 5 to 20% by weight.

  The beta-glucosidase-containing component used in the present invention is advantageously shaped as fine particles coated with a polymer derived from a living body and / or an enteric polymer in order to prevent degradation by digestive juices secreted from the digestive tract. It is. The addition amount of the processed product of the cells in fine particles is preferably 0.05 to 10% by weight, more preferably 0.2 to 3% by weight.

  As the above-mentioned polymers derived from living organisms, proteins such as collagen and gelatin, polysaccharides such as starch, alginic acid, cellulose, chitin and chitosan, and organic acids and amino acid polymers such as polylactic acid and poly-L-lysine can be used. In particular, chitosan, which is considered to have a strong interaction with vitamin C and a high vitamin C scavenging ability, is more advantageous as a living body-derived polymer that coats a processed product of wolfberry. The chitosan that is the deacetylated form is most advantageous.

Examples of enteric polymers include Eudragit, neutralized hydroxypropylmethylcellulose phthalate polymer (HPMCP), hydroxypropylmethylcellulose phthalate HP50 (HPMCP-HP50) (USP / NF)
220824), HP55 (HPMCP-HP55) (USP / NF type 20000731) and HP55S, available from Shin-Etsu Chemical, Coateric ™ (polyvinyl acetate phthalate) (Colorcon Ltd.), Sureretic ™ (polyvinyl acetate phthalate) (Colorcon)
Ltd .. ), Aquateric ™ (cellulose acetate phthalate) (FMC Corp.), or the like. Such an enteric polymer is a polymer that can be dissolved in the intestine, and generally begins to be soluble at a pH of 5.5 or higher, and generally has a maximum dissolution rate at a pH higher than 6.5. Since the vitamin C efficacy-enhancing composition according to the present invention is in a form containing two types of single agent, the beta-glucosidase-containing material contained in almond fruits and the like is first released by dissolution of the film, and after some time difference, provitamin C It is desirable to select the wolfberry fruit extract that is released to the next stage.

  Regarding coating with the above-mentioned living body-derived polymer and / or enteric polymer, it is desirable that both the ascorbic acid-2-O-beta-glucoside-containing component and the beta-glucosidase-containing component are coated. This is because even if only one of the components is coated, an effective action and effect cannot be obtained. In other words, since beta-glucosidase that functions as an enzyme that converts to vitamin C is a protein, it degrades with pepsin in gastric juice without coating, resulting in loss of enzyme activity, such as ascorbic acid-2-O-beta-glucoside This is because it is considered that provitamin C is diluted to reach the intestine without coating.

  Furthermore, it is also desirable to form an enteric capsule in which an ascorbic acid-2-O-beta-glucoside-containing component and a beta-glucosidase-containing component are separately contained in a capsule formed of an enteric polymer. In order to fractionate the amphoteric components, each component can be coated as described above, or each component can be accommodated in each chamber as a capsule having compartments. If formed in this way, the ascorbic acid-2-O-beta-glucoside-containing component and the beta-glucosidase-containing component are not only in contact with each other for the first time in the intestine, but the contact probability of both of them is further increased, resulting in an effect. This is because it can be increased.

  Reducing substances that can stabilize the product of the present invention include catechin, catechin derivative, rutin, rutin derivative, taurine, thiotaurine, eggshell membrane extract, gallic acid, gallic acid derivative, quercetin, quercitrin, yeast extract, ganoderma lucidum extract , Yashajitsu extract, Gennosho extract, Buttonpi extract, Melissa extract, Parsley extract and Dicoppi extract, Superoxide dismutase, Mannitol, Bilirubin, Cholesterol, Tryptophan, Histidine, Retinol and its derivatives (retinol palmitate, acetic acid Retinol, etc.), retinal and its derivatives, vitamin A such as dehydroretinal, carotenoids such as carotene, astaxanthin, lycopene, cryptoxanthin, thiamines (thiamine hydrochloride, thiamine sulfate, etc.) Riboflavins (riboflavin, riboflavin acetate, etc.), pyridoxines (pyridoxine hydrochloride, pyridoxine dioctanoate, etc.), flavin adenine nucleotides, cyanocobalamin, folic acids, nicotinic acids (nicotinamide, benzyl nicotinate, etc.), cholines, etc. Vitamin B of ergocalciferol, cholecalciferol, dihydroxystanal and other vitamin D, tocopheryl phosphate, tocopherol and derivatives thereof (dl-α (β, γ) -tocopherol, dl-α-tocopherol acetate Nicotinic acid-dl-α-tocopherol, linoleic acid-dl-α-tocopherol, succinic acid dl-α-tocopherol, etc.) and other vitamin E derivatives, ubiquinone, coenzyme A, vitamin K, and other reducing vitamins Kind , Dibutylhydroxytoluene, butylhydroxyanisole and the like.

  It is preferable to add a water-soluble polymer to the vitamin C efficacy-enhancing composition of the present invention, but in this case, in particular, the viscosity stability can be improved, and the viscosity can be decreased or increased over time. Prevent and keep constant. Conventional methods for improving the temperature stability, antioxidant, precipitation suppression, and / or color invariance of L-ascorbic acid or a derivative or salt thereof in the composition are also effective.

  Tablets, granules, capsules are suitable as the dosage form of the vitamin C efficacy enhancing composition of the present invention, but creams, lotions, lotions, gels, sprays, powders, poultices, bath preparations, hair restoration agents, shampoos. , Rinses, packs, drinks or eye drops are also included and are not particularly limited.

  Examples of the present invention will be described below. However, the examples are only a part of the present invention, and the present invention is not limited only by the examples.

<Example 1>
As a source of provitamin C, normally cultivated coconut fruit is dried with cold air at 25 ° C. or lower, and pulverized with a miller. Freeze-thaw with a nizer and liquid nitrogen, pulverize again with a miller to form a powder of 20-60 microns, and freeze-dry at a vacuum pressure of 60-220 kg / cm2 to prepare a powder. On the other hand, almond berries as an enzyme for converting provitamin C to vitamin C is pulverized and dried without heating to prepare a dry powder. One gram of dried wolfberry powder and 100,200,500,1000 milligrams of each dried almond fruit powder were mixed with vitamins per guinea pig (8 animals per test area) fasted for 18 hours. Mix in 10 grams of C-free feed, eat it by eating out, add vitamin C in the blood 12 hours after ingestion, add reducing agent (16 mM dithiothreitol), inject 20 μL into the ODS column, and use HPLC. As a result of separation and coulometric ECD, the control animals that received a diet that did not contain wolfberry or almond fruit had a concentration of 3.17 ± 0.18 ppm, compared to almonds against the dried powder of wolfberry. In the case of a feed mixed with 0%, 1%, 2%, 5%, and 10% of the dry powder of corn, the blood vitamin C concentration is 3.24 ± 0.25ppm, 6.07 ± 0.39ppm, They were 9.74 ± 0.54ppm, 11.60 ± 0.83ppm, 9.33 ± 0.74ppm. It has been found that the vitamin C concentration in the blood is increased by mixing the dried powder of wolfberry and the dried powder of almond.

<Example 2>
The coconut fruit as a plant material cultivated by the usual method is dried with cold air at 25 ° C. or lower, crushed with a miller, added twice the amount of cold water to the resulting powder, stirred with a vortex mixer, Centrifuge the suspension to store the supernatant. Add the same amount of cold water to the precipitate, stir with a vortex mixer, and centrifuge the suspension to store the second supernatant. Combine with the first supernatant and freeze-dry to obtain a water-soluble extract of wolfberry (KK) (Kk-bGAsc). On the other hand, food-related materials, especially almonds (Am), which contain enzymes that convert provitamin C to vitamin C, are crushed in a mortar with non-heated and unflavored fruits and centrifuged after ethanol sterilization. Five times the amount of milli-Q ultrapure water is added to the resulting precipitate and extracted (25 ° C., 1 hour, stirred), and lyophilized (Am-bGase). Beta-Glucosidase from Oriental Yeast Co., Ltd. (4-4-1 Minami Suita, Suita-shi, Osaka 564-0043, Phone 06-6384-1222)
Almond (Oy-bGase) has a specific activity of 36.8U / mg
This use is also preferred. KK-bGAsc, a water-soluble extract of wolfberry, dissolved in 100mg / mL Chitosan aqueous solution derived from coconut shell to a concentration of 70mg / mL, mixed with an equivalent amount of toluene, rotated at 100rpm under reduced pressure of an aspirator and treated with an evaporator Then, Am-bGAsc fine particles having a particle size of 80 mesh or less are prepared. On the other hand, as two types of almond extracts, Am-bGase and Oy-bGase are each dissolved in a 100 mg / mL shellfish-derived chitosan aqueous solution at a concentration of 70 mg / mL, mixed with an equivalent amount of toluene, and 100 rpm under reduced pressure of an aspirator. And processed with an evaporator to prepare Am-bGase and Oy-bGase fine particles having a particle size of 80 mesh or less. These are mixed to obtain a fine-grain mixture of a substance containing ascorbic acid-2-O-β-glucoside which is provitamin C and an enzyme containing substance which converts it into ascorbic acid.

<Example 3>
A fine-grained mixture of 20: 1 weight ratio of an ascorbic acid-2-O-β-glucoside containing provitamin C prepared in Example 2 and an enzyme containing it that converts it to ascorbic acid was aged 21-57. 12 Japanese people are divided into 4 groups of 3 people per group, and an intake test is carried out in a rotation system so that all groups receive 4 types of intake forms over an interval of 4 weeks and 16 weeks. The blood vitamin C 2 hours after ingestion was compared. The measurement method is to remove protein using MolCut and collect total vitamin C (sum of reduced and oxidized forms) as described in the literature (J. Cancer Res. Clin. Oncol. 126: 511-518 (2000)). It measured by the same method. As a result of separating by ODS column HPLC and measuring by coulometric ECD, it was 8.43 ± 0.42ppm in the control group that took the placebo with only the base from which the active ingredient was removed. 11.37 ± 1.32ppm in the group ingesting only KK-bGAsc fine granules, 16.85 ± 1.40ppm in the group ingesting a mixture of KK-bGAsc fine grains and almond-derived Am-bGase fine grains, only Am-bGase fine grains The ingested group was 7.52 ± 0.98ppm. It has been found that blood vitamin C concentration is increased by mild vitamin C conversion by almond fruit water-soluble component β-glucosidase to ascorbic acid-2-O-β-glucoside, a water-soluble component of wolfberry fruit.

<Example 4>
An edible material as an inclusion of an enzyme that converts provitamin C into vitamin C, in particular, an enzyme preparation derived from Aspergillus oryzae, the brand name Biozyme (Bz) is also used. Enzyme manufactured by Amano Kogyo Co., Ltd., and α-amylase preparation biozyme for food addition, are F10SD, A, and L, each of which is used for breadmaking and L for manufacturing maltose / oligosaccharides. F10SD powder (BzF10D) and L (BzL) powder are selected. Next, the dried rice koji mold powder manufactured by Bioc Co., Ltd. was washed with 50 ~ 100% ethanol and diluted 2-10 times with Milli Q ultrapure water (Kj). After washing with 70% ethanol, 5 mL of Milli Q ultrapure water is added to the precipitate obtained by centrifugation, and the suspension stirred with a vortex mixer is freeze-dried. These are each dissolved in 100 mg / mL swine-derived gelatin aqueous solution at a concentration of 70 mg / mL, mixed with an equivalent amount of toluene, rotated at 100 rpm under reduced pressure of an aspirator, and processed with an evaporator. Was prepared. These are mixed to obtain a 20: 0.3 weight ratio fine-grained mixture of ascorbic acid-2-O-β-glucoside, which is provitamin C, and an enzyme-containing material that converts it into ascorbic acid.

<Example 5>
Ascorbic, the provitamin C prepared in Example 4
A 20: 0.3 weight ratio fine-grain mixture of acid-2-O-β-glucoside-containing material and enzyme-containing material that converts it into ascorbic acid, 4 groups of 12 people aged 21-57 years old, 3 people per group Divided into 4 groups, and over 16 weeks with an interval of 4 weeks, the intake test was conducted in a rotating manner so that all groups received 4 types of intake, and blood vitamin C 2 hours after intake Compared. The measurement method is to remove protein using MolCut and collect total vitamin C (sum of reduced and oxidized forms) as described in the literature (J. Cancer Res. Clin. Oncol. 126: 511-518 (2000)). It measured by the same method. As a result of separating by ODS column HPLC and measuring by coulometric ECD, it was 6.98 ± 0.70ppm in the control group that received a placebo with only the base from which the active ingredient was removed. 8.91 ± 2.87ppm in the group receiving only KK-bGAsc fine granules, 16.85 ± 4.08ppm in the group receiving KK-bGAsc fine granules and biozyme BzF10D fine granules, KK-bGAsc fine grains and koji mold for rice bran The group that received the Kj mixture was 17.26 ± 1.86 ppm. It was found that blood vitamin C concentration was increased by the slow conversion of vitamin C due to β-glucosidase action of biozyme and koji mold enzyme for rice bran on ascorbic acid-2-O-β-glucoside, a water-soluble component of wolfberry .

<Example 6>
In Example 2, an enteric polymer (HPMCP (hypromellose phthalate ester) manufactured by Shin-Etsu Chemical Co., Ltd.) was used in place of the coconut shell-derived chitosan aqueous solution. Enteric polymer coating component (hereinafter “Coating Kk-bGAsc”) of water-soluble extract (Kk-bGAsc) and almond-derived conversion enzyme (Am-bGase) enteric polymer (HPMCP (Shin-Etsu Chemical Co., Ltd.)) Hypromellose phthalate ester)) coating component (hereinafter, coating Am-bGase), a mixture containing both is further coated with Shin-Etsu Chemical HPMCP (hypromellose phthalate ester) Created.

  The following composition is divided into 4 groups of 12 1-57-year-old Japanese people, 3 groups of 1 group, and each group is divided into 4 types over a period of 16 weeks with an interval of 4 weeks. Ingestion tests were carried out in a rotation system so as to receive the above-mentioned intake forms, and blood vitamin C after 2 hours of intake was compared. The measurement method is to remove protein using MolCut and collect total vitamin C (sum of reduced and oxidized forms) as described in the literature (J. Cancer Res. Clin. Oncol. 126: 511-518 (2000)). It measured by the same method. The results of separation by ODS column HPLC and measurement by coulometric ECD are shown below.

(1) 24.79 ± 1.53 ppm in the group that ingested a further coating of a mixture in which both Kk-bGAsc fine granules and Am-bGase fine granules were separately coated.
(2) It was 14.66 ± 2.79 ppm in the group ingesting those coated only with Kk-bGAsc fine granules (not coated with Am-bGase fine granules).
(3) In the group ingesting those coated only with Am-bGase fine particles (not coated with Kk-bGAsc fine particles), it was 9.03 ± 1.45 ppm.
(4) In the group ingesting a single agent mixture that was not coated (Kk-bGAsc fine granules and Am-bGase fine granules) and were individually coated in enteric capsules, it was 19.33 ± 1.87 ppm. It was.

Claims (7)

  One or more kinds selected from the group consisting of wolfberry seeds, persimmon leaves, and processed products as a source of provitamin C, and / or an enzyme that converts provitamin C to vitamin C and / or A vitamin C efficacy enhancing composition comprising one or more selected from the group consisting of the enzyme-containing substances.   The composition for enhancing vitamin C efficacy according to claim 1, wherein the processed product of wolfberry as a source of provitamin C is a dried powder of coconut and / or a water-soluble extract.   The content of the enzyme that converts provitamin C to vitamin C is one or more selected from the group consisting of almond fruit, the processed product, the dried powder, and the water-soluble extract. The composition for enhancing vitamin C efficacy according to claim 1.   The vitamin C efficacy-enhancing composition according to claim 1, wherein the enzyme that converts provitamin C to vitamin C is beta-glucosidase.   Fine particles coated with dried powder of coconuts as a source of provitamin C and / or the water-soluble extract with biopolymers and / or enteric polymers, and almond berries as a beta-glucosidase-containing substance The composition for enhancing vitamin C efficacy according to claim 1, comprising fine particles obtained by coating a dry powder / water-soluble extract of edible meal with a biopolymer and / or an enteric polymer.   Ascorbic acid-2-O-beta-glucoside contained in microparticles coated with dried powder of coconut as a source of provitamin C and / or the water-soluble extract with a biopolymer and / or an enteric polymer Beta contained in microparticles coated with a biopolymer and / or an enteric polymer of 1 mol (about 338 mg) of almond fruit dry powder / water-soluble extract as a beta-glucosidase-containing substance -A composition for enhancing vitamin C efficacy, comprising 20-500 units (international unit) of the enzyme active ingredient of glucosidase.   The vitamin C according to claim 5 or 6, wherein the biopolymer is one or more selected from the group consisting of collagen, gelatin, chitin, chitosan, polylactic acid, and poly-L-lysine. Efficacy enhancing composition.