JP2007137791A - Novel physicochemically harmonized bactericidal disinfectant solution - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a device for permanently inhibiting the appearance of resistant bacteria, since an iron ion-containing bactericidal solution has excellent characteristic features, but the problem of the appearance of resistant bacteria is unavoidable. <P>SOLUTION: The bactericidal disinfectant solution is obtained by generating micro nanobubbles in an aqueous solution containing a trivalent iron ion (Fe<SP>3+</SP>) in an amount of 500-1,500 ppm and L-ascorbic acid in an amount of 500-2,000 ppm as the main components, and at least one kind of sorbic acid, benzoic acid, a salt thereof, and a p-hydroxybenzoic acid ester in an amount of 200-2,000 ppm. The bactericidal disinfectant solution is also obtained by generating micro nanobubbles in the above aqueous solution, and thereafter sealing the solution to store it for 60-90 days. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

The present invention relates to a sterilizing / disinfecting solution in which micro / nano bubbles are generated in an aqueous solution containing trivalent iron ions (Fe ³⁺ ) and L-ascorbic acid as main components.

At present, there are alcohols, phenols, halogen compounds, quaternary ammonium salts, biguanide drugs, aldehydes, etc., which are widely used in medical institutions, but are safe and have low toxicity. Unfortunately, there is no one that satisfies the conditions of being effective against various types of bacteria, fungi and viruses, being able to be used in a wide range, having excellent storage stability and being inexpensive. In light of this situation, we invented a new type of disinfectant that is friendly to living organisms and the environment, and that shows a steep effect on pathogenic bacteria. (See Patent Document 1). The gist of this is from the preservatives and fortifiers that have antibacterial action, but also have a trivalent iron ion (Fe ³⁺ ), which is a mineral that is useful to the human body, and are approved as food additives. By selecting and combining these, the high bactericidal effect and the above-mentioned conditions were cleared.

Specifically, the content of trivalent iron ions (Fe ³⁺ ) is 500 to 1500 ppm, the content of L-ascorbic acid is 500 to 2000 ppm, sorbic acid, benzoic acid and their salts, and paraoxybenzoic acid. An antibacterial solution containing at least one or two or more acid esters, the content of which is 200 to 2000 ppm, and, as an example, ferric chloride hexahydrate (FeCl ₃ .6H _A bactericidal solution composed of ₂ O) 1000 ppm, L-ascorbic acid 1000 ppm, and potassium sorbate 500 ppm can be mentioned. The main features of this iron ion-containing sterilizing solution are as follows.
(1) Bactericidal effect (i) It shows a rapid effect on general bacteria and fungi (shows an effect within 10 seconds).
(Ii) Effective against acid-fast bacteria within 1 minute of contact.
(Iii) Effective for contact with bacterial spores for 1 minute to 120 minutes.
(2) Toxicity (i) Application to the skin: It was applied to the mouse foot pad twice a day for 2 months, but no abnormality was observed. Thereafter, it was used several times per day for disinfection of human fingers, but no abnormality occurred.
(Ii) Oral administration (po): 1 ml / mouse was administered, but no toxicity was observed. The LD ₅₀ was estimated to be 10 ml / mouse.
(Iii) Intraperitoneal administration (ip): LD ₅₀ was 1 ml / mouse.
(Iv) The effect on cultured cells (animals) was no longer recognized as being impaired by a 1000-fold dilution.
(V) No abnormalities were found when used for gargle.
(3) Stability The sterilizing effect of this iron ion-containing sterilizing solution showed stability over a long period (about 3 years).
(4) Use This iron ion-containing sterilizing solution is composed only of compounds that have been approved as food additives, and they showed a high sterilizing effect only by synergistic action without producing new compounds. Therefore, it can be used for sterilization of fresh foods such as instruments, equipment and vegetables as well as disinfection of fingers and wounds. In addition, as a quasi-drug, it can also be applied to medicated soaps, medicated toothpastes, odor control agents, and mouthwashes.

On the other hand, as a disinfection method, not only a chemical disinfection method but also a physical disinfection method is adopted. That is, as a physical disinfection method that is routinely adopted,
(1) Incineration (the most reliable method)
(2) High-pressure steam sterilization (autoclave)
(3) Dry heat sterilization (160 ° C, 30 minutes, etc.)
(4) Boil disinfection (5) Filter filtration method (6) Ultraviolet disinfection (absorbed and destroyed by microbial DNA)
(7) Radiation sterilization (Use of gamma rays penetrates into the substance.)
(8) Although ultrasonic waves exist, as a physical disinfection method that can be fused with the chemical disinfection method, ultrasonic waves that are relatively easy to handle and show stability were investigated. As a result, we focused on ultra-fine bubbles (micro / nano bubbles) that are closely related to ultrasound. Micro / Nano Bubbles create a space between water molecules when water is vibrated strongly with ultrasonic waves, and when the sound waves have negative pressure, and countless number of ultra-fine bubbles are generated when the inside becomes vacuum. This springs out and is crushed and destroyed (collapse phenomenon) by its own surface tension and the next positive pressure, and disappears (see, for example, Patent Document 2). Such a phenomenon is repeated in micro / nano bubbles. It has become clear that this characteristic exhibits useful functions such as bactericidal action, organic matter decomposition, cleaning action, and biological activation. This phenomenon occurs also when water and air are swirled at an ultra-high speed or by a discharge phenomenon (see, for example, Patent Document 3). On the other hand, in a normal bubble, the bubble becomes larger while rising in the water and can be repelled on the surface of the water.

JP 2000-44417 A JP 2002-143885 A Japanese Patent No. 3397154

  As described above, although it is an iron ion-containing sterilizing liquid having excellent characteristics, it is not necessary to look at examples of antibiotics, and bacteria produce an enzyme that decomposes it against compounds that damage themselves, Protect yourself. In fact, it is common knowledge that resistant bacteria of alcohol and biguanide drugs cause nosocomial infections. Therefore, this iron ion-containing sterilizing solution cannot avoid the emergence of resistant bacteria. In order to cover this phenomenon as much as possible, it is recommended that trace amounts of essential oils and surfactants such as Timor and Eucalyptus that have antibacterial action are added, but these additives are no different from chemical substances. . That is, in order to permanently prevent becoming resistant bacteria, some device is required, and this is the subject of the present invention.

  As a result of intensive studies on the sterilizing effect of microorganisms and the relationship between the above-described sterilizing liquid containing iron ions and micro / nano bubbles, the present inventors have provided a sterilizing / disinfecting liquid containing micro / nano bubbles.

That is, as a main component, the content of trivalent iron ions (Fe ³⁺ ) is 500 to 1500 ppm, the content of L-ascorbic acid is 500 to 2000 ppm, sorbic acid, benzoic acid and their salts, and paraoxy A sterilizing and disinfecting solution characterized in that micro / nano bubbles are generated in an aqueous solution containing at least one or more benzoic acid esters, the content of which is 200 to 2000 ppm. It is preferable that micro / nano bubbles are generated at a rate of 100 ml or more / minute, and the total is preferably 15 L or more, and it is particularly preferable to generate micro / nano bubbles from a depth of 50 cm or more from the liquid surface of the aqueous solution.

In the second aspect of the present invention, the content of trivalent iron ions (Fe ³⁺ ) is 500 to 1500 ppm and the content of L-ascorbic acid is 500 to 2000 ppm as the main components, and sorbic acid, benzoic acid and Micro- / nano bubbles are generated in an aqueous solution containing at least one or more of those salts and paraoxybenzoic acid ester, and the content thereof is 200 to 2000 ppm, and then sealed and stored. It is a sterilizing and disinfecting solution, and after micro / nano bubbles are generated in the aqueous solution, it can be stored sealed for 60 to 90 days. Micro / nano bubbles are generated at a rate of 100 ml / min or more with respect to 100 L of the aqueous solution. It is preferable to be 15 L or more, and micro / nano bubbles are introduced from a depth of 50 cm or more from the surface of the aqueous solution Raw Sarukoto is particularly preferred.

  The sterilizing solution of the present invention contains micro / nano bubbles, so that the sterilizing power of the iron ion-containing sterilizing solution is remarkably increased by increasing the penetrating power to living tissue, and sterilization of spores in a short time Even for chronic infectious diseases that are difficult for drugs to penetrate, such as specimen sterilization, rhinitis, periodontal disease, athlete's foot, etc., more effective sterilization became possible, opening the way to early cure. This can give treatment hope to those who suffer from infectious diseases. In particular, it can be widely used for disinfecting fingers, skin, nose, mucous membranes, wound sites, various specimens, surgical sites, hospital rooms, furniture, instruments, articles and the like.

The iron ion-containing sterilizing solution referred to in the present invention means that the content of trivalent iron ions (Fe ³⁺ ) is 500 to 1500 ppm and the content of L-ascorbic acid is 500 to 2000 ppm, and sorbic acid, benzoic acid And a sterilizing liquid containing at least one or more of paraoxybenzoates and salts thereof and having a content of 200 to 2000 ppm.

  The bactericidal disinfectant referred to in the present invention refers to a liquid disinfectant having a bactericidal action. Here, bactericidal action is an irreversible effect that damages the cells of microorganisms and causes cells to die and return to their original state. Changes. The disorder means that cells are destroyed by reacting quickly with enzymes and proteins necessary for microorganisms to inhabit, or by depriving cells of moisture. In addition, cell walls and cell membranes are destroyed by strong oxidizing power, and some sterilizing agents induce lysis by coagulating, depositing, and denaturing microbial proteins.

  The micro / nano bubble referred to in the present invention means a bubble having a diameter of 50 μm or less and has a negative charge, and therefore attracts bacteria and viruses. Next, when the bubble disappears (this is called “crushing phenomenon”), it instantaneously becomes high temperature and pressure and water decomposes, generating free radicals (mainly active oxygen), which are bacteria and viruses. Destroy. Since these destroy bacteria and viruses at the gene level, like ultraviolet rays, resistant bacteria like antibiotics do not occur.

  In the present invention, after micro / nano bubbles are generated, in order to store them in a sealed state, the sterilizing / disinfecting solution containing micro / nano bubbles can be put into a container by a normal method and the stoppers can be sealed. it can. There is no restriction | limiting in particular in a preservation | save period, After storing in a container, it can be used anytime. However, since it can be stored for 60 to 90 days in practice, it is preferably used during that time.

  Details of the present invention will be described based on test examples and examples, but the gist of the present invention is not limited thereto. In order to generate micro / nano bubbles, there is no particular limitation as long as it is a device that can create bubbles having a diameter of 50 μm or less, and any device can be used, but in the test examples and examples of the present invention, Used an M2-MS / SUS type manufactured by Nano Planet Research Laboratories Ltd., which has a discharge liquid flow rate of 7 L / min and a maximum bubble generation amount of 1 L / min.

(Test Example 1)
About 100 L of water is put into a water tank of width 70 cm × depth 30 cm × height 50 cm, and S. aureus (E. coli) and E. coli (E. coli) are each 1 × 10 ⁸ cells / water tank as representative bacteria. Water / ml was added, and micro / nano bubbles were generated for 60 minutes with a maximum bubble generation amount (1 L / min) at a discharge liquid flow rate of 7 L / min using a Nano Planet Research Laboratory M2-MS / SUS type. The state in which S. aureus and E. coli decreased at this time was measured over time, and is shown in Table 1. As is clear from Table 1, micro / nano bubbles generated in water certainly showed bactericidal action, but are very slow compared to general chemical disinfection methods. That is, the number of viable bacteria was 1 / 20,000 to 1 / 50,000 of the original number of bacteria in 60 minutes, but even if the generation of micro / nano bubbles was continued, complete sterilization was not achieved. Therefore, the purpose can be achieved if it is about sterilization, but it cannot be expected when sterilization is desired.

(Test Example 2)
Based on the results of Test Example 1, the relationship between the generation amount of micro / nano bubbles, the generation time, and the bactericidal ability was examined. Using the same water tank and water volume as in Test Example 1, 1 × 10 ⁸ pieces of S. aureus / ml of water in the water tank was added, and the phenomenon that S. aureus decreased to 1 × 10 ⁴ pieces / ml of water in the water tank was used as a reference. The results are shown in FIG. As is clear from FIG. 1, in order to exert a sufficient sterilizing ability, when the generation amount of micro / nano bubbles is 500 ml / min, at least 30 minutes or more, when 1000 ml / min, 15 minutes or more, 200 ml / min In this case, the generation amount and the generation time were in an inversely proportional relationship, and the generation amount of micro / nano bubbles per minute was 500 ml / min × 30 minutes = 15000 ml. In the case of 100 ml / min, it was simultaneously found that there was no bactericidal action even if micro / nano bubbles were generated for a long time.

(Test Example 3)
Next, the relationship between the position (depth) at which micro / nano bubbles occur in water and the bactericidal action was investigated. This is because it is presumed that micro / nano bubbles generated deeper cause crushing phenomenon in water and increase free radical release. 1 × 10 ⁸ S. aureus / ml of water in a water tank were added to a 100 L water tank with different depths, and micro / nano bubbles were generated at 1 L / min for 30 minutes, and the number of living bacteria was measured. The results are shown in FIG. As can be seen from FIG. 2, even when micro / nano bubbles are generated from a position having a depth of 10 cm, the bactericidal action is exhibited, but the action is weak. However, when micro / nano bubbles are generated from a position of 30 cm depth, the number of bacteria is 1 × 10 ⁵ cells / ml of aquarium water, and when micro / nano bubbles are generated from a position of 40 cm depth, the number of bacteria is 2 × 10 ^4. When micro / nano bubbles are generated from a position of 1 m / aquarium water, 1 m depth, the number of bacteria is 5 × 10 ³ cells / ml, and when micro / nano bubbles are generated from a position of 3 m depth, the number of bacteria is It decreased to 2 × 10 ³ pieces / ml aquarium water. This suggests that the generation position (depth) is not a very important factor for sterilization ability compared to the generation amount of micro / nano bubbles. Accordingly, the various tests and examples carried out thereafter used a 100 L water tank having a depth of 50 cm, that is, the same type of water tank used in Test Example 1. It has been clarified that the relationship between the generation amount of micro / nano bubbles in water, the generation time, and the sterilizing ability is applicable as it is even when a sterilizing liquid described later is used.

(Test Example 4)
In general, it is well known that disinfectants have a reduced bactericidal effect due to contamination of organic substances, particularly proteins. Therefore, the same amount of skim milk and yeast extract is mixed as protein, and the mixture is added in amounts of 10 ppm, 100 ppm and 1000 ppm, and at the same time 1 × 10 ⁸ S. aureus and E. coli / ml of aquarium water, respectively. In addition, micro / nano bubbles were generated for 60 minutes with a maximum bubble generation amount (1 L / min) at a discharge liquid flow rate of 7 L / min using an M2-MS / SUS type manufactured by Nano Planet Research Ltd. The bactericidal effect on S. aureus and E. coli at this time was examined. The results are as shown in Table 2, and in the presence of organic substances, when the concentration was 10 ppm, the bactericidal effect was hardly affected, but when the concentration was 100 ppm or more, the difference in degree was That's why my sterilization ability declined. At a high concentration of 1000 ppm, about 0.1% of the bacteria survived without being destroyed.

(Test Example 5)
About 100 L of the culture solution was placed in the same water tank as in Test Example 1 and set to 37 ° C. Next, S. aureus and E. coli were each added at 1 × 10 ⁷ cells / ml of culture solution, and the maximum bubble was produced at a discharge liquid flow rate of 7 L / min using M2-MS / SUS type manufactured by Nano Planet Research Laboratory. Micro / nano bubbles were generated at a generated amount (1 L / min) for 48 hours, and the change of the added bacteria was measured over time. The composition of the culture solution was a meat extract broth prepared by dissolving 10 g of meat extract, 10 g of peptone, and 2 g of NaCl in 1 L of water and adjusting the pH to 7.2. As is apparent from Table 3, the bacteria decreased without growing even immediately after the occurrence of micro-nano bubbles, but the degree was slow, approximately 1/2 after 1 hour, 1/1000 after 48 hours, 48 hours. Later, 1 / 10,000 bacteria continued to survive. In addition, even if the test was continued as it was, all the bacteria did not die.

(Test Example 6)
About 100 L of water is poured into the same water tank as in Test Example 1, and the upper marginal calculus plaque, which is an aggregate of guarded bacteria, is collected, and is directly pulverized into a 200 μm-thick section and a diameter of 10 μm. After submerging in aquarium water, 500 ml of micro / nano bubbles were generated, 1 part was collected every 15 minutes, the number of viable bacteria contained in the plaque was measured, and the results are shown in Table 4. The plaques were collected by filtration with a filter and dried in a sterile box. As is apparent from Table 4, the viable bacteria in the plaque decreased as if there were no barriers, in other words, at the same rate as the naked bacteria. In other words, the micro-nano bubbles have weak sterilizing ability but have strong permeability to living tissues and objects. In the case of a conventionally used disinfectant, for example, when a Hibiten solution is used, the bacteria on the plaque surface are almost killed within 3 minutes, but the bacteria living inside are resistant to immersion for 60 minutes and are about 30 to 70. % Could survive.

  What has become clear from the test results of Test Examples 1 to 6 described above is that the disinfection ability of micro / nano bubbles is very weak compared to general chemical disinfection methods and physical disinfection methods. The recognized sterilization or disinfection is far from being a little different. The most important example is Test Example 6.

(Test Example 7)
Spores in bacteria are covered with a thick film of 3-4 layers of outer skin, spore shell, outer layer, spore wall and have low permeability, so it has a strong resistance to the influence of the outside world and has a sharp chemical disinfection There is currently no law. Therefore, the test was performed using spores as subjects. The spores were separated and collected by the following method. (1) The dry sand of Kawahara was sampled and dried well with hot air at 100 ° C. for several hours while stirring it. First, vegetative cells were sterilized. 1 g of that was put into water and immediately filtered through a filter. A portion of the filtered water was applied to a slide glass and stained according to a conventional method, and the number of spores in 1 g of the mixture was measured from the number of stained spores. The spores at this time contained various miscellaneous spores. (2) 3 g of yeast extract, 10 g of polypeptone, 10 g of glucose, 0.1 g of MnSO ₄ .5H ₂ O, 3 g of NaCl, and 15 g of agar were dissolved in 1 L of water and Bacillus pumilus was applied to a spore-forming medium adjusted to pH 7.2. Cultured aerobically for 7 days at 0 ° C. to form spores. In addition, 50 g of tryptone, 5 g of polypeptone, 5 g of sodium thioglycolate, 5 g of glucose, 1 g of Na ₂ HPO ₄ and 15 g of agar were dissolved in 1 L of water, and Clostridium perfringens was applied to a spore formation medium adjusted to pH 7.2. And anaerobically cultured for 7 days to form spores. The resulting spores were heated at 70 ° C. for 15 minutes, vegetative cells were inactivated, washed twice with sterile water, then dried under reduced pressure and mixed with normal starch. The number of spores in 1 g of starch was measured in the same manner as in (1). About 100 L of water was poured into the same water tank as in Test Example 1, and the collected spores were added at 1 × 10 ⁸ per 1 ml of water in the tank, generating 1 L / min of micro / nano bubbles, 120 minutes over time. 1 ml of aquarium water was sampled, and the state of spores decaying and dying was measured by a conventional dilution method. The results are shown in Table 5. As is clear from Table 5, the bactericidal effect was exhibited regardless of the type of spore, and the effect was slightly inferior to the bactericidal effect on normal vegetative cells shown in Test Example 1, but 1% of the spore was 10 minutes later. After 15 minutes, 2 to 2.5% of spores were destroyed, 5% after 30 minutes, and 35 to 40% of spores after 60 minutes. In other words, it was demonstrated that micro / nano bubbles exert a bactericidal action to some extent even with respect to strong spore tissues due to their penetrating power. This is a truly surprising phenomenon from the standpoint of spore disinfection.

  The bactericidal action involving micro / nano bubbles is as described above. However, the effect of the combined use with the existing disinfectant was examined on the bactericidal action. However, alcohol, halogen compounds and phenolic compounds are rather affected. It became clear that the action decreased. However, in the “iron ion-containing sterilizing solution” described in Japanese Patent Application No. 2000-44417, the sterilizing effect was remarkably enhanced. Furthermore, it became clear that the “iron ion-containing sterilizing solution” containing micro / nano bubbles can retain its strong sterilizing power for several months in a sealed state unless it is opened. When the “iron ion-containing sterilizing liquid” containing micro / nano bubbles is opened, the sterilizing liquid that remains to be used all at once is gradually returned to the original sterilizing action.

(Production Example 1)
At least one or two kinds of sorbic acid, benzoic acid and paraoxybenzoic acid ester having a content of III-valent iron ions (Fe ³⁺ ) of 500 to 1500 ppm and a content of L-ascorbic acid of 500 to 2000 ppm Among the sterilizing liquids containing the above and having a content of 200 to 2000 ppm, a sterilizing agent comprising ferric chloride hexahydrate (FeCl ₃ .6H ₂ O) 1000 ppm, L-ascorbic acid 1000 ppm, and potassium sorbate 500 ppm The solution was called “BC-Fe”.

(Production Example 2)
About 100 L of the “BC-Fe” sterilizing solution manufactured in Manufacturing Example 1 was poured into the same water tank as in Test Example 1, and the discharge liquid flow rate was 7 L / min using the M2-MS / SUS type manufactured by Nano Planet Research Laboratory. Then, a sterilizing / disinfecting solution in which micro / nano bubbles were generated for 60 minutes at the maximum foam generation amount (1 L / min) was poured into a 500 ml reagent bottle and referred to as “ON-MNB”.

(Production Example 3)
About 100 L of the “BC-Fe” sterilizing solution manufactured in Manufacturing Example 1 was poured into the same water tank as in Test Example 1, and the discharge liquid flow rate was 7 L / min using the M2-MS / SUS type manufactured by Nano Planet Research Laboratory. A solution in which micro / nano bubbles were generated for 60 minutes at a maximum bubble generation amount (1 L / min) was poured into a 500 ml reagent bottle and stored for 1 month in a tightly-sealed bottle, which was referred to as “CL-MNB”.

Example 1
In order to see the effect of the sterilizing solution against pathogenic bacteria, the suspension concentration of the test bacterium is adjusted to 1 × 10 ⁸ cells / ml of physiological saline solution, and the weight of the sterilizing solution is 2 wt. % Respectively. One platinum ear was picked over time, inoculated into each enrichment medium, cultured in an optimal environment, and the bactericidal effect was observed by the presence or absence of growth of each fungus. The results when the general bacteria were contacted for 10 to 60 seconds are shown in Table 6, and the results when the spore-forming bacteria were contacted for 1 to 120 minutes are shown in Table 7. Since many of the general bacteria could be sterilized within 10 seconds, only representative bacterial species are shown in Table 6. As is clear from Tables 6 and 7, BC-Fe killed many common bacteria in 10 seconds. Also, E. faecalis and Ps. Aeruginosa, which do not die in 10 seconds, all died within 10 seconds in ON-MNB and CL-MNB containing micro / nano bubbles. In addition, mycobacteria that took about 1 minute to kill and C. albicans that took more than 1 minute were completely killed in 20 to 30 seconds. The early spores were within 1 minute, and all collapsed and killed after 60 minutes at the latest.

(Example 2)
In Test Example 4, the sterilizing effect of the micro / nano bubbles when the organic substance was mixed was examined. The sterilizing effect when the organic substance was mixed was also examined for the sterilizing liquid of the present invention. As organic solutions, skim milk and yeast extract of 0.2% by weight and 1% by weight, respectively, were prepared, and 1 ppm, 50 ppm, and 100 ppm were added to the disinfectant, respectively, and S. aureus ( MRSA) and E. coli O-157 suspension concentrations adjusted to 1 × 10 ⁸ cells / ml of physiological saline were added at 2% by weight. The contact time with the test bacteria was 10 seconds to 60 seconds, and 10 μl of the added solution was collected over time and inoculated into the medium. The cells were cultured at 37 ° C., and the bactericidal effect was observed with and without the growth of the bacteria. The results are shown in Tables 8 and 9. As can be seen from Tables 8 and 9, when the concentration of organic substances is high, BC-Fe required 60 seconds for sterilization, but ON-MNB and CL-MNB containing micro / nano bubbles sterilized within about 20 seconds. We were able to. By adding a small amount of antibacterial metal ions such as Cu ²⁺ described in JP-A No. 2000-44417, essential oils such as Titry oil exhibiting antibacterial properties, or surfactants to the organic solution, the sterilizing ability is improved. Needless to say, it has been strengthened.

  Cases of vigorous growth of bacteria in the natural environment occur in nutrition-rich foods, not to mention cases of food poisoning. Therefore, in such a case, it was observed how much the sterilizing / disinfecting solution of the present invention can inhibit the growth of bacteria and can be sterilized. That is, each sterilizing and disinfecting solution was sprayed in the process of decaying food left after cooking, and the subsequent change of bacteria was observed. Usually, it is impossible to spray a disinfectant directly on cooked food, but since BC-Fe contains food additives as a component, it is considered that the practicality will rise depending on the result.

(Example 3)
After cooking "Kayaku rice", "Chilled guy", "Tuna and squid sashimi" and "Meat potato" are allowed to stand at 28 ° C for 24 hours, and then each food is sprayed with each sterilizing liquid evenly. One part of each was collected from immediately after spraying for 24 hours, homogenized with a homogenizer, and the number of viable bacteria in 1 g of food was measured according to a conventional method. The decaying process naturally varies depending on the cooking method, ingredients and environment, but in this example, “Kayaku-rice” is 1.2 × 10 ⁵ pieces / g after being left for 24 hours, and “Chiller” is 8 × 10 ⁷ pieces. / G, “Tuna and squid sashimi” had a count of 3.5 × 10 ⁸ cells / g, and “Meat potato” had a count of 1.5 × 10 ⁷ cells / g. This is a significant increase compared to the number of viable bacteria immediately after cooking, especially “Chiller” and “Tuna and squid sashimi”, which already had some rotting odor. Table 10 shows changes in the number of spoilage bacteria in the food. As is apparent from Table 10, the number of viable bacteria immediately after spraying is 1/100 to 1/1 in BC-Fe when sprayed with a bactericidal disinfectant solution in a state in which food decay has progressed considerably, depending on the type of dish. It was found that it decreased to about 1/1000 after 1000 hours and 1/1000 to 1 / 100,000 after 2 hours. However, after 24 hours, the effect diminished and the number of bacteria showed a slightly increasing tendency. As for “Kayaku rice”, the decrease in the number of viable bacteria was somewhat inferior because the sprayed disinfectant was not sufficiently distributed. On the other hand, when ON-MNB was sprayed, it decreased by an order of magnitude compared to BC-Fe, continued to decrease after 24 hours, and reached a level close to complete sterilization. That is, it was shown that ON-MNB reaches not only the surface of food but also the bacteria invading inside with its strong penetrating power and exerts bactericidal power. Although CL-MNB was somewhat inferior to ON-MNB, it showed results according to this.

Example 4
A general-purpose disinfectant is said to be ineffective for excrement generally referred to as a specimen and is inappropriate for use. Therefore, the effectiveness of the disinfectant of the present invention for a specimen was examined.

(1) Sputum: Spoons were collected from several people, mixed, then stirred well, then divided into two equal parts, one of which was given a prescribed amount of sputum solubilizing agent, and left for 30 minutes. On the other hand, the viable cell count was measured as it was (with viscosity). Thereafter, a bactericidal disinfectant having an amount twice the weight of the specimen was added and stirred well, and the number of viable bacteria in the specimen was measured over time. The results are shown in Table 11. As is apparent from Table 11, when the solubilizer was administered, BC-Fe decreased to about 1/100 after 5 minutes and to 1 / 1,000,000 after 60 minutes. In other words, although it could not be completely sterilized, it could be used for disinfection. In contrast, when ON-MNB and CL-MNB were added, the number of viable bacteria decreased to 1/1000 after 5 minutes and then rapidly decreased, and viable bacteria could be recognized after 60 minutes. It demonstrated its power for complete sterilization. On the other hand, when each bactericidal disinfectant solution is added to a candy that does not use a solubilizer, BC-Fe decreases to about 1/10 after 5 minutes and only about 1/1000 after 60 minutes. It was very difficult to say. On the other hand, in the case of ON-MNB, although it does not reach complete sterilization, it was found that the bacteria survived very little and could be used practically. CL-MNB also showed results according to this. Here too, it was shown that the tissue penetration of BC-Fe containing micro-nano bubbles is considerably increased.

(2) Feces: 1 × 10 ¹¹ bacteria / g of intestinal bacteria lived in the collected feces. BC-Fe was added twice as much as the sample weight, stirred well, and the number of viable bacteria in the sample was measured over time. The results are shown in Table 11. As can be seen from Table 11, 1/50 after 5 minutes addition, about 1/1100 million after 60 minutes, i.e. 1 × 10 ² to 2 × 10 ³ pieces / g, The fungus was never killed. With addition of ON-MNB, it is decreased by an order of magnitude more than BC-Fe, that is, 1 × 10 ² / g or less, and similar results can be obtained with addition of CL-MNB, which can be effective for sterilization of feces. It became clear.

(3) Pus: When the causative bacteria of the pressure ulcers were observed for the sterilization of MRSA-based pus and Pseudomonas aeruginosa-based pus, the significant difference in susceptibility to the disinfectant was found. There wasn't. The addition of BC-Fe rapidly decreased the causative bacteria and did not reach complete death, but after 60 minutes, the number decreased to 1 × 10 ³ cells / g or less. In contrast, when ON-MNB was added and when CL-MNB was added, all causative bacteria died after 15 minutes. When reviewing the above results, it was concluded that BC-Fe was somewhat effective, ON-MNB was highly effective, and CL-MNB was effective in accordance with the effect of sterilizing and disinfecting samples. It was. Rather than adjusting the concentration of the sterilizing and disinfecting solution to 2 to 3 times that of normal use and adding it to the specimen, it was more effective to increase the dose at the normal concentration. Furthermore, the same test was conducted with a conventional general-purpose disinfectant, 70% alcohol, and biguanide drugs, but the results were not ineffective but very difficult to say.

(Example 5)
The effect when the germicidal disinfectant of the present invention was used for gargle was examined. Measure the number of viable bacteria in 1 ml of saliva before gargle, then gargle 3 times, that is, 1 minute for 20 seconds once with a disinfectant solution, and immediately after that, collect saliva after 15 and 60 minutes. The number of viable bacteria contained in 1 ml of saliva was measured, and the results are shown in Table 12. As a control, gargle was performed using tap water with 0.6 ppm residual chlorine. As can be seen from Table 12, the number of bacteria of 5 × 10 ⁸ cells / ml just before gargle with tap water was reduced to 2 × 10 ⁷ cells / ml of 1/25 immediately after gargle. It was revealed that the number recovered as time passed and became 1 × 10 ⁸ cells / ml after 60 minutes. In the case of gargle with BC-Fe, the number of bacteria of 7 × 10 ⁸ cells / ml immediately before the gargle decreased to about 1/2000 of 4 × 10 ⁴ cells / ml immediately after gargle, and 60 minutes later. There was almost no increase in the number of bacteria. Next, when gargled with ON-MNB, it decreased to 1/1 million immediately after gargle, and after 60 minutes, some growth of bacteria was observed, but remained at a low level. When gargled with CL-MNB, although the effect was slightly inferior to ON-MNB, it was an approximate result. In addition, when using benzalkonium chloride and iodohol (isodine solution) recommended as daily gargles, the reduction is only 1/100 to 1/1000, and boric acid and commercially available mouthwashes are also 1 From the viewpoint of sterilization, it was found that the level was about the same as that of tap water.

(Example 6)
The sterilizing effect on the skin surface of the sterilizing and disinfecting solution of the present invention was examined by spraying and wiping.

(1) The disinfection liquid was widely sprayed on the skin surface of the hand, and the effect was examined. Immediately after spraying, the area of 10 cm ² was wiped off with sterilized gauze, and the number of bacteria was measured by a swapping method. Next, the pre-sprayed area was wiped off by 10 cm ² while moving after 15 minutes, 30 minutes and 60 minutes, and the number of bacteria was measured by a swapping method. The results are shown in Table 13. As is clear from Table 13, the tap water spray naturally did not change the number of bacteria before spraying, but when BC-Fe was sprayed, the number of bacteria immediately after spraying rapidly decreased to 1/1300, Although it further decreased with the passage of time and decreased to 1/1000 after 30 minutes, it was still not completely killed. The same tendency was observed when ON-MNB was sprayed, but the condition became almost aseptic after 30 minutes. However, after 60 minutes, a small number of bacteria presumed to be falling bacteria in the environment were detected. Also, CL-MNB showed similar results to ON-MNB.

(2) A bactericidal antiseptic solution was immersed in a sterilized gauze, the skin surface of the hand was wiped with the gauze, and the number of bacteria was measured by the same procedure as the spray method. As is clear from Table 13, when wiped with tap water, the number of bacteria decreased to 1/17, and BC-Fe rapidly decreased to 1/2300 immediately after wiping, and to 1/7000 after 15 minutes. After that, the bacteria started to increase gradually. Unlike the case of spraying, the application state of the sterilizing and disinfecting solution was thin, so it was assumed that it was volatilized at an early stage and the effect lasted short. In addition, when wiped with ON-MNB and CL-MNB, it was almost sterilized immediately after wiping, but viable bacteria appeared after 15 minutes and increased considerably after 60 minutes. Many of them were speculated as mere falling bacteria.

(Example 7)
Bactericidal disinfectant was sprayed on the floor surface (linoleum), which was considerably noticeable in the same manner as the skin surface, 100 cm ^{2 of} each was wiped off with sterilized gauze, and the number of bacteria was measured by the swap method. As is clear from Table 14, all showed a tendency similar to the results of the spray method in Example 6. That is, although live cells of ^{2 × 10 7 ~3 × 10 7} / 100cm 2 before treatment were present, when sprayed with BC-Fe, reduced to 1/6000 immediately after, after 60 minutes It became 1 / 30,000. In contrast, in ON-MNB and CL-MNB, the floor surface was almost aseptic after 15 minutes. In addition, when the floor was wiped, the results were in accordance with the skin surface wiping method in Example 6.

(Example 8)
Recently, large companies have begun to enter the soil remediation business and are expected to grow into a large industry in the future. At present, however, pollution purification methods mainly consist of chemical treatment or physical treatment. . However, in the future, to promote the growth of plants such as crops, fruit trees, and flowers, and to control pests, soil is sterilized at the same time as purification, and then microorganisms suitable for each crop are distributed to spread efficient cultivation methods. It is presumed that Therefore, the soil in the field was collected, put in 4 small flower pots, and then sprayed with a watering funnel until each soil was sufficiently wet. Thereafter, the soil was collected over time, and the number of bacteria living per 1 g of soil was measured. As a result, the bacteria that had inhabited 5 × 10 ⁹ cells / g in the soil distributed BC-Fe, immediately after that, 5 × 10 ⁷ cells / g, that is, 1/100, and 2 minutes after 60 minutes. × 10 ³ pieces / g, that is, 1 / 2500,000. Thereafter, it gradually decreased and decreased to 1 × 10 ³ cells / g, that is, 1/5 million after 12 hours, but even here, all the bacteria were not killed. On the other hand, in the case of ON-MNB, 1/5000 immediately after distribution, 1/25 million (2 × 10 ² pieces / g) after 60 minutes, and 1/50 million (1 × 10 ² after 12 hours). It was drastically reduced to ² / g), and was almost at the level of complete sterilization. CL-MNB had similar results. All the sterilizing disinfectants showed high effectiveness in soil sterilization, but ON-MNB and CL-MNB exhibited a lot of effectiveness in addition to that.

Example 9
Surprisingly, it is not known that vegetables account for about 30% as a causative ingredient of food poisoning. Be especially careful when eating raw vegetables. Therefore, it is important to thoroughly wash vegetables to prevent food poisoning. This time, radish, cracked shellfish, cabbage and lettuce were used as test vegetables, and the number of viable bacteria before and after washing per unit area (cm ² ) was measured over time. This is shown in FIG. The washing time was 20 seconds, and the control was washed with running tap water. As apparent from Table 15, the number of attached bacteria varies depending on the vegetables, but it was reduced to approximately 1/1000 only by washing with tap water. However, the bacteria gradually increased in the state of being left as it was, and returned to 1/10 to 1/20 before washing after 5 hours. This is considered to be the total of the bacteria that grow by using vegetables as nutrients and the bacteria that adhere from the environment. On the other hand, when it was washed with BC-Fe, no bacteria were detected immediately after that, and it was not detected even after 1 hour, or even a very small number of bacteria. However, it increased to 1/100 of the number of bacteria before washing after 5 hours. This phenomenon suggests that the cleaning effect with the liquid is extremely high, and the effect is sustained to some extent. The reason is considered that a film of the liquid component is formed on the vegetable surface, which acts as a barrier. In addition, when ON-MNB and CL-MNB were used, the further high washing | cleaning disinfection effect was exhibited and the aseptic state continued even if 5 hours passed.

(Example 10)
Fish is well known as a food ingredient for food poisoning because of its rapid decay due to many pathogens and spoilage bacteria lurking not only on the body surface but also inside the scales. In particular, we Japanese prefer raw food, and this kind of incident has no spare time. This time, we investigated the cleaning effect of the disinfectant solution on mackerel, squid, and sardines, which progressed particularly quickly. In the case of tap water, the surface of the fish was lightly rubbed for 30 seconds in running water. In the case of a sterilizing liquid, the same treatment was performed for 30 seconds in a container filled with the liquid. Thereafter, the liquid was washed away with tap water and left at 28 ° C. The detected bacteria were classified into the Vibrio genus and other bacteria that are closely related to fish, and the number of viable bacteria before washing and the number of viable bacteria after washing were measured over time, and the results are shown in Table 16. . As is apparent from Table 16, in tap water, immediately after washing, the number of bacteria attached to the fish was greatly reduced to about 1/100. However, if left as it is, the remaining bacteria began to grow again, and the total number of bacteria after 5 hours returned to the level before washing. In particular, the growth of Vibrio was remarkable and increased several times before washing. On the other hand, when washed with BC-Fe, the bacteria were almost killed, and even when left at 28 ° C., the bacteria including the Vibrio genus grew only slightly. In the case of ON-MNB and CL-MNB, similar to the easy example, a more pronounced bactericidal effect was developed, which lasted for several hours. This means that many food poisoning cases can be solved by using this disinfectant. Moreover, the taste of the original fish was maintained without any change in taste due to the sterilizing solution.

(Example 11)
When vegetable seeds are contaminated with bacteria and fungi, they often fail to grow normally after germination or indirectly contaminate the soil, resulting in crop failure. Therefore, it is desirable that the seed is in a clean state. Shellfish radish, leek and honey bee seeds were collected, and the cleaning effect of the disinfectant was investigated. In the tap water, each child was dipped in running water for 1 minute and then lifted. In the case of the sterilizing / disinfecting liquid, it was immersed for 1 minute. The treated seeds were then allowed to germinate by being soaked shallowly in distilled water at 22 ° C. The number of bacteria attached to one seed after washing was measured, and the results are shown in Table 17. As is apparent from Table 17, the attached bacteria rapidly decreased only by washing with tap water, but not all were removed, and 30 to 50% of the bacteria before washing were reattached after 5 hours. On the other hand, when immersed in each sterilizing / disinfecting solution, the attached bacteria were almost completely annihilated and only a few bacteria were reattached after 5 hours. Moreover, the influence which it has on germination shortened germination time 10 to 15% compared with tap water, and also germination time improved. This fact indicates that the water penetration into seeds has increased.

(Example 12)
The cleaning effect was examined on door knobs (made of stainless steel) and sanitary ware for urine, which are contaminated by bacteria and fungi by many unspecified people. In either case, the facilities in the hospital were used and utilized as usual from immediately after washing, and the number of adherent bacteria and the state of contamination thereafter were observed over time. The results are shown in Table 18. As is apparent from Table 18, the number of bacteria attached to the knob before washing was 6 × 10 ⁵ per 10 cm ² , but when it was washed with tap water, it immediately decreased to 5 × 10, that is, 1 / 10,000. . However, the bacteria began to attach again during use and returned to the original number (7 × 10 ⁵ ) after 24 hours. In contrast, when wiped with BC-Fe, viable bacteria were not detected immediately after, and the bacteria gradually reattached over time, but 4 × 10 ³ cells even after 24 hours, that is, Even after 48 hours of the original 1/100, only 2 × 10 ⁴ , ie, the original 1/25 adherent bacteria were observed. This means that when the object to be cleaned is an instrument, the antibacterial action lasts for a certain period of time. When wiped with ON-MNB, sterility continued for 2 hours immediately after. Thereafter, only 7 × 10 ² bacteria, ie, about 1/1000 of the original number of bacteria, could be detected after 24 hours. Similar results were obtained for CL-MNB.

  Next, the change in the number of viable bacteria in the urinal for urinal is that when washed with tap water, urine can be a good medium for bacteria, so it will return to the number of bacteria before washing in 24 hours, and the bottom will also be slightly colored, A yellowing occurred. On the other hand, when BC-Fe is used for washing, even when 24 hours have passed, about 1/30 of the number of bacteria before washing, when ON-MNB and CL-MNB are used, an additional digit As described above, only a small number of bacteria could recover and proliferate. In addition, it was seen that the re-deposition of the dirt was not noticeable regardless of which sterilizing solution was used, and kept clean after washing.

(Example 13)
The cleaning effect of the disinfectant was observed on the body of 4 indoor dogs (2 dachshunds, 2 terriers) and 4 Shiba dogs raised outdoors. The whole body other than the head was soaked for 3 minutes in each of the tap water warmed to 32 ° C. and the three sterilizing / disinfecting liquids also warmed to 32 ° C., and the body was washed while holding the body with a sponge. Next, the body was pulled up and dried while brushing the hair with a dryer. Immediately thereafter, 24 hours later, the number of bacteria adhered to the body surface was measured, and the fur and body odor were checked. The results are shown in Table 19. As is clear from Table 19, the average number of bacteria adhering to the body surface of 4 dogs raised indoors was 6 × 10 ⁵ per 10 cm ² , but the number of bacteria immediately after washing with tap water was After 1/60, the bacteria started to grow, and after 24 hours, it was almost in the state before washing. In contrast, when washed with BC-Fe, immediately after that, it drastically decreased to 1/1000. Even after 24 hours, it remained at the level of 1/250 of the number of bacteria before washing, and the antibacterial action was observed to continue. It was done. In addition, there was an effect of reducing body odor, and the hair was brushed as if using a rinse, and the appearance was excellent. Next, when it was washed with ON-MNB, it was almost aseptic immediately after that, but after that, the bacteria gradually started to increase, and after 24 hours, it became 1/500 level before washing. Similar results were shown for CL-MNB. In all cases, the number of bacteria was one digit less than that of BC-Fe. In addition, the deodorizing effect and the state of body hair were not changed to BC-Fe. In the case of an outdoor dog, two to three times as many bacteria as the indoor dog adhered, but the cleaning effect was similar to that of the indoor dog.

(Example 14)
The cleaning effect of the bactericidal disinfectant solution was observed on the body of 2 domestic cats as in the case of dogs. The method was the same as in the case of the dog of Example 13. The results were as shown in Table 20, and the fungus fate and body hair showed similar results to indoor dogs, but the deodorizing effect of body odor felt higher than in dogs. .

  As described above, the cleaning effects of vegetables, seeds, fish, instruments, pet hair, etc. using three types of disinfecting disinfectants showed high effectiveness, but ON-MNB and CL containing micro / nano bubbles -Both sterilizing disinfectants of MNB showed a cleaning effect one rank higher.

  The invasion and proliferation of bacteria into the body is called infection, but once the bacteria start to grow in the body, the body shows various reactions (called infection), fever, redness, Various phenomena such as swelling appear. At this point, the use of drugs such as antibiotics is appropriate, and if the site penetrates sufficiently, the infection should be cured. However, various phenomena such as erasing the time of use, improper usage, discontinuation, and insufficiently reaching the local area prevent the eradication or elimination of pathogenic bacteria from the infected site, In many cases, treatment is unsuccessful. In this way, infectious diseases are sometimes prolonged or even worsened, and they are transformed into diseases that are still difficult to treat in the medical field. Typical examples include sinusitis, periodontal disease, and athlete's foot (dermatodermatosis), which can be used to treat intractable infections by using a bactericidal antiseptic solution for pretreatment of those treatments. We examined whether.

(Example 15)
One example of the development of chronic sinusitis is to catch a cold, get nasal discharge, and for some reason infected with pathogenic bacteria, start to multiply in the sinuses and produce toxins, and the body reacts to this Then, onset, pus is formed, and the aspect gradually increases in intensity. Nasal discharge (pus) is more viscous and less likely to be excreted from the body, drugs are difficult to break through, and the local reaction gradually increases with chronicity, such as secondary reaction (mucosal mucosa) Treatment becomes extremely difficult. If you don't use surgery, pass a metal tube through your nose, inhale and wash pus, then spray anti-inflammatory agents and antibiotics, and take long-term use of new macrolide antibiotics (months) Is a common treatment method. However, in many cases, it takes a longer period of time for the mucosal thickening to return to its original state, or it does not reach complete cure. 8 patients with chronic sinusitis whose main causative bacteria are Staphylococcus aureus were divided into 4 groups of 2 each, and (i) physiological saline, (ii) BC-Fe, (iii) ON-MNB, ( iv) A nasal washes were performed once daily with CL-MNB, and immediately after that, a necrolide antibiotic (trade name: roxithromycin) was sprayed and a clinical trial method in which a normal half amount was taken for a long time was adopted. The therapeutic effect was diagnosed by (a) subjective symptoms, (b) pathological findings of nasal mucosa, (c) radiographs. The results are shown in Table 21. As is apparent from Table 21, when BC-Fe was used compared to the case of washing with only physiological saline, the effect appeared from about one month later. In the findings as well as the X-ray findings, the disease was gradually alleviated. In addition, when ON-MNB or CL-MNB was used for nasal washing, the symptoms started to relieve at an earlier stage than with BC-Fe, and in 6 months the pathological and radiological findings Was almost completely cured.

(Example 16)
Periodontal disease is a disease in which the surrounding tissues that support the teeth, i.e., gums, cementum, periodontal ligament, alveolar bone, etc., become inflamed and gradually collapse. It is caused by bacteria that live in the groove in the neck of the tooth, which is the boundary of the teeth, and form plaques (plaque). The toxins and enzymes produced by these bacteria first cause gingivitis, which develops into the teeth Peripheral pockets are formed, where plaque grows and periodontal disease develops. As the symptom progresses, the pocket spreads deeply, inflammation spreads to the root of the tooth, gradually destroys the tissue, bleeds from it, begins to feel bad breath, and quickly loses teeth. There are various factors that make it difficult to treat periodontal disease, and one of them is that it is difficult to always keep the oral cavity clean and suitable for the growth and propagation of bacteria. A strong bacterium produces insoluble polysaccharides with adhesiveness and has strong adhesion to teeth and gingiva, and this substance also acts as a barrier to protect against periodontal disease-causing bacteria. Therefore, the current initial treatment of periodontal disease is mainly a treatment called scaling that removes plaque and tartar that becomes a hotbed of periodontal disease, and then injecting antibacterial agents and anti-inflammatory agents into the periodontal pocket and fixing the current situation Although a method of regenerating tissue is adopted, radical cure is difficult when inflammation is deep, and periodontal disease is recognized as a typical model of chronic infection.

  The present inventors filed a patent application in the United States in 2004 as “a novel treatment method for periodontal disease”, the content of which was injecting BC-Fe into a gingival pocket, then washing with water, and then a special lactic acid bacterium. It was related with the treatment method by a combination of the two of filling. In Test Example 6 described above, it was proved that the sterilizing effect on plaque was superior to BC-Fe in that the sterilizing ability including osmotic power was better by using ON-MNB or CL-MNB. (Injected into the periodontal pocket), the same effect as in vitro, and whether it can help to cure early periodontal disease.

  Nine patients with moderate periodontal disease progression (pocket depth around 10mm) and 9 patients deep in the pocket (pocket depth 15mm or more) are divided into 3 groups of 3 Seed germicidal antiseptic solution is infused into periodontal pocket for 10 days every day, thereafter injecting germicidal antiseptic solution, left for 5-10 minutes, then washed with water, then filled with special lactic acid bacteria preparation described in US patent application A series of treatments were performed once a day every day. The average treatment results are shown in Table 22 and Table 23. The main causative bacteria in all patients were P. gingivalis, P. intermedia, B. forsythus, and A. actinomycetemcomitans, either alone or in combination. As is clear from the results in Tables 22 and 23, there was no significant difference in the first stage of treatment in which three kinds of bactericidal antiseptic solutions were individually injected into the periodontal pockets for 10 days, but they were used in combination with lactic acid bacteria. In the second stage of treatment, periodontal disease, which was previously impossible to completely cure, is more likely to use ON-MNB or CL-MNB than to use BC-Fe as a pre-treatment disinfectant solution. It was confirmed that it was almost completely cured in a short period of about 2 months.

(Example 17)
I have been suffering from foot athlete's foot for many years, I went to a doctor and tried various treatments until now, but it did not improve at all, especially for 20 athlete's foot athletes who have been in trouble in the summer, and conducted a trial for 2 months. Carried out. The clinical trial method consists of (i) a patient who immerses the affected area in BC-Fe in gold dalai and ON-MNB in a bathtub for 5 minutes, and then wipes with a paper towel, and (ii) some conventional drug treatment (antifungal agent) Patients who were receiving (bifonazole or clotrimazole) were soaked in BC-Fe or ON-MNB disinfectant solution for 5 minutes and then applied the drug for a total of four trials. More than half of the subjects also suffered from onychomycosis (onychomycosis), and the nail was discolored and thickened and the tip collapsed. The complete cure of athlete's foot itself is quite difficult, and experts recognize that it is particularly troublesome for nail athlete's foot. Table 24 shows the symptom of ringworm disease of the subject and the results of microscopic examination and culture test of ringworm that is presumed to be prevalent in the skin or nails collected from the affected area. Table 25 summarizes the average therapeutic effects of the above four clinical trial methods. In many cases, there is no direct damage even if you are affected by athlete's foot, so you can leave it and change to chronic, which is difficult to treat. In particular, it is prominent in the case of nail fungus. As is apparent from Table 25, by using [ON-MNB] and [ON-MNB + athlete's foot drug] for treatment, in the case of intercostal type or small blister type, it becomes almost completely cured in about two months, and the keratinized type It is noteworthy that the course of treatment is good, and in the case of nail athlete's foot, the prospect of healing has been opened by continuing this treatment method.

  The disinfectant solution of the present invention is used for disinfecting fingers, skin, nose, mucous membranes, wound sites, various specimens, surgical sites, hospital rooms, furniture, instruments, articles, etc., local disinfection such as infants and bedridden sick people as wet tissue, Cleaning sterilization of kitchen utensils such as cutting boards, cleaning sterilization of toilets, etc., quasi-drugs, medicated toothpaste, medicated soaps, anti-odor agents, bath preparations, mouthwash, etc. For pets and animals, it can be applied to various resin products, tiles, pottery, building materials, etc. as disinfecting body and feet, deodorizing agents, body hair treatments, disinfecting pet goods such as bait boxes, and antibacterial materials. In addition to the above, it has a wide range of uses such as cleaning and disinfection of fresh food, disinfection of eggshells, and disinfection of soil, and it seems to have infinite possibilities. In the 21st century, the field of biotechnology will continue to develop in the future, but it is indispensable for its application development, which is a sterilizing and disinfecting solution that is convenient and has a certain effect. They are eagerly craving.

It is the graph which showed generation | occurrence | production time and generation amount of micro / nano bubbles. It is the graph which showed the depth of micro nano bubble generation | occurrence | production location, and the number of living microbes.

Explanation of symbols

1. 1. Area showing sterilizing power Area without sterilizing power

Claims (5)

As a main component, the content of trivalent iron ions (Fe ³⁺ ) is 500 to 1500 ppm and the content of L-ascorbic acid is 500 to 2000 ppm, and sorbic acid, benzoic acid and their salts, and paraoxybenzoic acid A sterilizing and disinfecting solution characterized in that micro / nano bubbles are generated in an aqueous solution containing at least one or two or more esters and having a content of 200 to 2000 ppm. As a main component, the content of trivalent iron ions (Fe ³⁺ ) is 500 to 1500 ppm and the content of L-ascorbic acid is 500 to 2000 ppm, and sorbic acid, benzoic acid and their salts, and paraoxybenzoic acid A sterilizing and disinfecting solution characterized in that micro / nano bubbles are generated in an aqueous solution containing at least one or two or more of esters and the content thereof is 200 to 2000 ppm and then sealed and stored.   3. The sterilizing / disinfecting solution according to claim 2, wherein micro / nano bubbles are generated in the aqueous solution and then sealed and stored for 60 to 90 days.   The sterilizing / disinfecting solution according to any one of claims 1 to 3, wherein micro / nano bubbles are generated at a rate of 100 ml / min or more per 100 L of the aqueous solution to a total of 15 L or more. The sterilizing / disinfecting solution according to any one of claims 1 to 4, wherein micro / nano bubbles are generated from a depth of 50 cm or more from the surface of the aqueous solution.

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