JP2007170997A - Virus-suspension collection method - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a method for efficiently and safely treating fecal samples in the case of detecting viruses in feces. <P>SOLUTION: The method for collecting virus suspensions uses a fecal collection container comprising both a tubular fecal collection tube and a filtrate tube. The fecal collection tube is provided with a dissolving and housing chamber inside for housing a dissolving solution for dissolving feces; one end part in which a fecal collection rod for collecting feces is to be inserted; and the other end part provided with a filter for filtering a suspension. The filtrate tube is to be mounted to the other end part of the fecal collection tube. After the fecal collection rod covered with feces is inserted in the fecal collection container, the feces adhering to the fecal collection rod are dissolved in the dissolving solution. Then the fecal collection container is centrifuged to collect only a liquid layer in the filtrate tube passed through the filter in the method for collecting virus suspensions. The method for collecting virus suspensions is provided to enable the collection of feces to the collection of a virus suspension with one and the same fecal collection container. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention relates to a method for collecting a virus suspension, more specifically, an invention relating to an efficient method for collecting a virus suspension from feces using a specific stool collection container.

Digestive viruses associated with diarrhea, vomiting, etc., such as norovirus, sapovirus, rotavirus, adenovirus, etc. are present in the stool of those infected with the virus, and stool specimens are used to diagnose these infections. It has been. For viral nucleic acid testing using gene amplification methods such as PCR of these viruses (RT-PCR in the case of RNA viruses), a centrifugal supernatant of about 10% suspension of a stool specimen is prepared, A necessary amount of the supernatant-containing layer containing the viral nucleic acid is dispersed, and a viral nucleic acid preparation step is performed thereon. At present, the pretreatment process for stool specimens involves melting the frozen stool collection container together with the stool specimen, collecting an appropriate amount of the lysate with a disposable loop (platinum ear), and hanging it on a microtube containing distilled water. They have been forced to do the cumbersome manual work of becoming cloudy. Many of the collected stool specimens are from patients with diarrhea, so the operation of separating a small amount of about 0.1 ml from watery stool was often forced, and in some cases a disposable syringe was also used. . Infectivity of the virus to be detected is often strong. For example, in the case of norovirus, dozens of virus particles are contained in the aerosol, and the virus may be infected just by inhaling it. In addition to droplet infection due to aerosols, contact infection accidents that are brought into the mouth through the hands of workers due to contamination such as droplet leakage or adhesion during inadvertent stool suspension work are also a large number of contracted specimens. It is recognized as a risk factor in the process of pretreatment. For this reason, all stool suspension operations must be performed in a safety cabinet in the P3 laboratory.
JP-A-8-292189 Japanese Patent No. 2668815

  In view of the above-described background art, the present invention is an object of the present invention to provide an efficient and safe processing means for a stool specimen when virus detection is performed. In order to solve such a problem, the present inventors once made a stool collection container capable of performing the steps from stool collection to stool specimen detection work safely and efficiently in the same stool collection container. In addition, intensive studies were conducted on how to use for virus detection.

  In order to solve such a problem, for example, a new stool collection container capable of satisfying the following conditions (1) to (4) is provided, and a virus stool specimen using the stool collection container is used. It is necessary to provide a separation method.

(1) A stool collection container that can already prepare a stool suspension with an appropriate concentration at the time of stool collection so that it is not necessary to prepare a stool suspension sample again after arriving at the laboratory. Be.

(2) Since many donors of stool specimens are already in diarrhea, the specimen provider himself / herself can easily collect the necessary amount of stool even from watery stool. thing. For that purpose, it is preferable to satisfy the following specific requirements 1) to 2).
1) The equipment used to collect stool (stool collection rod) shall be structured so that the prescribed amount can be taken without difficulty for both solid stool and watery stool.
2) In order to prevent the stool sample from being suspended in an excessive amount, it has a structure that can prevent the collection of an excessive amount of stool, and the stool sample overflows to the outer surface of the container and contaminates the surface and surroundings of the container. It has a structure that can prevent it from happening.

(3) A container made of a material that will not be damaged when the container after stool collection is stored frozen. Also, the container may be damaged due to volume expansion caused by freezing of the internal liquid, or the internal liquid from the joint may Leakage is suppressed.

(4) After arriving at the laboratory, the stool suspension sample is centrifuged to separate food digestion residues and bacterial cells in the stool suspension sample. At this time, the stool suspension sample is removed from the stool collection container. The stool collection container itself must have a structure that allows the virus suspension layer to be separated safely and efficiently so that it is not necessary to transfer to another test tube.

  Conventionally, as a stool collection container used in the fecal occult blood test and various virus tests, for example, the one shown in Patent Document 1 is known. This stool collection container has a container body whose both ends are opened and a middle is closed with a thin film, a cap member for closing one end of the container body, and a sample collection means (stool collection rod) attached to the other end of the container body. And a solution for dissolving feces is stored in a solution storage chamber between the cap member and the thin film. When the sample collection means is inserted from the open end of the container main body, excess stool adhering to the sample collection unit is wiped off with a thin film jar, and only a certain amount of stool is supplied to the solution storage chamber. Also, during inspection, the closure at the tip of the cap member is unscrewed by hand, and the suspension of the container body is filtered through a filter by pressing the inside of the container body by hand. It will be discharged.

  However, in the case of a conventional stool collection container represented by Patent Document 1, when the sample collection means is inserted into the solution storage chamber, the internal pressure of the solution storage chamber rises and the solution leaks down through the filter. The inspection may be hindered.

  Therefore, in Patent Document 2, a ridge that is provided inside the container body and that airtightly engages with the outer peripheral surface of the stool collection rod is provided on the separation wall that removes excess feces when the stool collection rod is inserted. In addition, a stool collection container is disclosed in which a recess that is longer than the axial length of the ridge of the separation wall is formed on at least a part of the peripheral surface of the stool collection rod so as to face the separation wall. According to the stool collection container described in Patent Document 2, the internal pressure of the container body, which has been raised by inserting the stool collection rod into the container body, can be reduced to normal pressure immediately before the completion of the insertion process. The suspension does not spout from the container body due to excessive pressure to contaminate the surroundings.

  However, even in the stool collection container described in Patent Document 2, since the internal pressure of the container body rises until just before the insertion process is completed, the dissolved solution leaks out of the container body through the filter. There is a fear.

  In order to solve the above-mentioned problems, the inventor has a dissolution storage chamber for storing a solution for dissolving feces, and a stool collection rod for collecting stool is inserted into one end, A stool collection container comprising a cylindrical stool collection tube provided with a filter for filtering the suspension at the end and a filtrate tube attached to the other end of the stool collection tube was used. A method for collecting a virus suspension, wherein a stool collection stick attached to a stool is inserted into the stool collection container, and then the stool attached to the stool collection stick is dissolved in the solution, Providing a method for collecting a virus suspension (hereinafter also referred to as the present collection method), which applies a centrifugal force of 1000 G or more and collects only the liquid layer in the filtrate tube that has passed through the filter. I came up with it.

  In this collection method, a stool collection container (hereinafter also referred to as a “stool collection container”) used for the purpose of detecting viruses in stool is provided with a solution storage chamber for storing a solution for dissolving stool, and the dissolution A tubular stool collection tube sealed at one end of the liquid storage chamber with a breakable membrane, a collection part provided with a stool collection part for collecting stool at the distal end side, with the proximal end side of the rod-shaped member as a gripping part A stool, and the stool collection part includes a tip part made of a pyramid having a top on an axis and a shaft part made of a prism, and forms a ridge line in which the pyramid and the prism are continuous. It is said.

  The conventional stool collection portion of the stool collection rod has a conical tip and a cylindrical column, so that when the membrane for sealing the solution storage chamber is broken through the stool collection membrane, There was variation in the direction.

  On the other hand, in this stool collection container, the tip of the stool collection part has a pyramid shape and the shaft part has a prism shape, so that the membrane can be sharply broken at the pyramid and the ridge line portion of the prism. The way of tearing does not vary consistently. Then, air flows from the gap formed between the broken and triangular film piece and the prismatic shaft portion, and the internal pressure of the solution storage chamber starts when the stool collection rod is inserted into the solution storage chamber. Is kept at normal pressure.

  Further, the filtrate tube mounted in a detachable state in the stool collection container is provided with a solution storage chamber for storing a solution for dissolving stool, and a stool collection rod for collecting stool at one end. And a cylindrical stool collection tube provided with a filter for filtering the suspension at the other end, and a filtrate tube attached to the other end of the stool collection tube. It is said.

  This collection method is characterized in that the virus suspension contained in the stool is collected using this stool collection container. Specifically, after the stool collection stick attached to the stool collection container main body is inserted into the stool collection container body, the stool attached to the stool collection stick is dissolved in the solution, and the container main body is subjected to a centrifugal separator at 1000 G or more ( Preferably, a centrifugal force of 2000 to 3000 G) is applied to collect only the liquid layer in the filtrate tube that has passed through the filter, and the liquid layer is used as a virus suspension.

  In this collection method, a centrifugal force is applied to the sealed main stool collection body using a centrifuge to collect only the liquid layer (virus suspension) in the filtrate tube that has passed through the filter. Therefore, it is not necessary to prepare a stool suspension sample, and droplet infection can be prevented. At this time, as described above, by setting the centrifugal force to 1000 times the gravitational acceleration (1000 G) or more, foreign substances including bacteria are trapped by the filter or settled on the bottom of the filtrate tube. The virus is suspended in the liquid layer of the filtrate tube, and the liquid layer can be used as the virus suspension.

  The target virus of this collection method is not particularly limited as long as it is a virus that can exist in feces, and examples thereof include norovirus, sapovirus, rotavirus, adenovirus, etc., but are not limited thereto. is not.

  In the stool collection container used in the method for collecting the virus suspension of the present invention, the stool collection part of the stool collection rod has a pyramidal shape at the tip and a prismatic shape at the shaft. When the membrane that seals the membrane is pierced by the stool collection portion, the manner in which the membrane is torn does not vary. Then, air flows from the gap formed between the fractured membrane and the prismatic shaft, and the internal pressure of the solution storage chamber can be maintained at normal pressure from the time when the stool collection rod is inserted into the solution storage chamber. it can.

  Then, centrifugal force is applied to the sealed container body using a centrifuge, and only the liquid layer in the filtrate tube that has passed through the filter needs to be collected as a virus suspension. Can be prevented. As a result, the work in the safety cabinet becomes unnecessary, and the safety of the worker can be improved and the work efficiency can be improved.

  This stool collection container is a prerequisite for carrying out this collection method. Using the best form of this stool collection container in this collection method is one of the best forms of this collection method. It means that.

  In this stool collection container, the shape of the stool collection part of the stool collection rod was formed between the broken membrane and the prismatic shaft part by making the tip part a pyramidal shape and the shaft part a prismatic shape. The air flows from the gap, and the internal pressure of the solution storage chamber is maintained at a normal pressure from the time when the stool collection rod is inserted into the solution storage chamber. preferable.

  In this stool collection container, it is preferable that a concave portion for accommodating feces is formed in a shaft portion of the stool collection portion, and a rib for partitioning the concave portion is provided in the concave portion.

  When the membrane that seals the solution storage chamber is pierced by the stool collection rod, the broken membrane piece may adhere to the stool collection rod. In particular, if the stool collection part is provided with a recessed part that accommodates feces, the torn film piece enters the recessed part and the air flow passage is blocked, not only the internal pressure of the solution storage chamber increases, but also the tear is broken. There is also a possibility that the film piece scrapes off the stool in the recess. In contrast, in the present stool collection container, by providing ribs that define the recessed portion in the recessed portion, the torn film piece is prevented from entering the recessed portion to ensure an air flow path, and the recessed portion. Make sure that feces in the club are not scraped off.

  In the stool collection container, both side portions of the shaft portion of the stool collection portion may protrude toward the opening side of the recessed portion.

  In the present stool collection container, since both side portions of the shaft portion protrude toward the opening side of the recessed portion, it is possible to secure a space serving as an air flow path on the upper surface of the recessed portion.

  Further, in the present stool collection container, a cylindrical sealing member is inserted into one end portion of the dissolution liquid storage chamber, the breakable membrane is stretched on one end surface of the sealing member, and the sealing member A convex seal portion may be provided around the outer peripheral portion of the first and second fins, and a plurality of fins may be provided radially above the seal portion.

  In this stool collection container, excess stool adhering to the stool collection rod is accommodated in the outer peripheral portion (between fins) of the sealing member, and a convex seal portion is provided around the outer peripheral portion of the sealing member. Thus, it is possible to prevent the stored excess feces from entering the solution storage chamber from the outer peripheral portion of the sealing member.

  As described above, the stool collection container, which is a stool collection container for carrying out the collection method, has a configuration in which a stool sample can be collected efficiently and safely in the same stool collection container. It is characterized by having.

Embodiments of the present invention will be described below with reference to the drawings.
The stool collection container exemplified in the present embodiment includes a tubular stool collection tube 10 in which a solution storage chamber 11 for storing a solution for dissolving stool is provided, and one end of the stool collection tube 10. A stool collection apparatus provided with a container body 40 composed of a filtrate tube 20 composed of a cylindrical container attached to the section, a proximal end side of a rod-shaped member as a gripping section 37, and a stool collection section 31 for collecting stool at the distal end It is composed of a rod 30. The shape of the container body 40 is shown in FIG. 1, and the shape of the stool collection rod 30 is shown in FIGS. In the following description, the direction in which the stool collection rod 30 is inserted into the stool collection tube 10 will be referred to as “down” and the opposite direction as “up” for convenience.

  The solution storage chamber 11 is provided in an intermediate portion of the stool collection tube 10, and a buffer solution is stored in the solution storage chamber 11 as a solution. Here, the solution is sterilized distilled water, physiological saline, phosphate buffer, or the like.

  While the upper end of the solution storage chamber 11 is sealed with a stocker 12 (sealing member), the lower end of the solution storage chamber 11 is partitioned by a filter 13 that filters the suspension, and in a state where no load is applied, The solution is prevented from leaking from the solution storage chamber 11 through the filter 13.

  A male threaded portion 15 is formed on the outer peripheral surface of the lower end portion of the stool collection tube 10 adjacent to the filter 13, and is screwed into a female threaded portion 21 formed on the inner peripheral surface of the opening of the filtrate tube 20. ing. On the other hand, an internal thread portion 14 is formed on the inner peripheral surface of the upper end portion of the stool collection tube 10 adjacent to the stocker 12, and an external thread portion 36 of the stool collection rod 30 to be described later is screwed together.

  The stocker 12 is formed in a cylindrical bottom portion 12d inscribed in the inner peripheral surface of the stool collection tube 10, a cylindrical portion 12b having a smaller diameter than the bottom portion 12d, and radially formed on the outer peripheral surface of the cylindrical portion 12b, and is longer than the cylindrical portion 12b. The plurality of fins 12c are integrally formed. A breakable film 12a made of polyethylene, elastomer, aluminum or the like is stretched on the upper end surface of the cylindrical portion 12b, and a convex seal portion 12e is provided on the outer peripheral surface of the bottom portion 12d.

  Excess stool adhering to the stool collection rod 30 is wiped off by the torn membrane 12a and the plurality of fins 12c and accumulates on the stocker 12. However, when the stool collection container is centrifuged, these excess stool is removed. It moves between the fin 12c and the fin 12c. At this time, since the seal portion 12e is provided around the outer peripheral surface of the bottom portion 12d, the excess stool stored does not enter the solution storage chamber 11 from the outer periphery of the stocker 12. As in the present embodiment, the stool collection rod 30 and the container body 40 can be separated before use, but before use, the form in which the male thread portion 36 of the stool collection rod 30 is screwed is selected. It is also possible to do. In this case, the membrane 12a is not provided, and the cross-sectional shape of the cylindrical portion 12b is circular, and the shape of the cylindrical portion is such that the columnar enlarged diameter portion 35 of the stool collection rod 30 can be fitted. It is preferable that

  The stool collection portion 31 of the stool collection rod 30 is composed of a tip portion 32 made of a quadrangular pyramid having an apex on the axis and a shaft portion 33 made of a quadrangular column, and the quadrangular pyramid and the quadrangular column form a continuous ridgeline. ing.

  In the case of the stool collection rod 30, the film 12a of the stocker 12 is broken in a cross shape at the ridge line portion of the stool collection part 31, and the way the film 12a is torn does not vary. Then, the air is circulated through the gap formed between the broken and triangular film piece and the shaft portion 33 made of a quadrangular column, and the solution is taken from the time when the stool collection rod 30 is inserted into the solution storage chamber 11. The internal pressure of the storage chamber 11 is kept at normal pressure.

  In the upper half portion and the lower half portion of the shaft portion 33, concave portions 33a that are long in the axial direction are formed on opposite surfaces, respectively, and feces are accommodated in the respective recessed portions 33a.

  The stool collection part 31 has a shape suitable for being scraped while being pressed against the stool and accommodated in the recessed part 33a without scooping the stool, and prevents the collection of stool of a specified amount or more in advance. However, it is possible to guide the feces not to be collected in a heap like the conventional sagittal stool collection part.

  Each recess 33a is partitioned into two rows by axially arranged ribs 33b to prevent the torn film 12a from entering the recess 33a and secure an air flow path. The feces in the recess 33a is prevented from being scraped off. Moreover, as shown in FIG. 3, both side portions 33c and 33c disposed in the axial direction with the rib 33b interposed therebetween protrude to the opening side of the recessed portion 33a, and serve as an air flow path on the upper surface of the recessed portion 33a. A void can be secured. In addition, the concave portion 33a is partitioned by the rib 33b to form a slit, and the width of the concave portion 33a is moderately narrow. Therefore, when collecting watery stool, the surface tension of the liquid is used. The stool collection rod 30 can be taken up in a state where the recessed portion 33a is filled with watery stool.

  Between the stool collection portion 31 and the gripping portion 37, a columnar reduced diameter portion 34, a columnar enlarged diameter portion 35, and a male screw portion 36 are sequentially formed from the stool collection portion 31 toward the gripping portion 37. . The reduced diameter portion 34 has a smaller diameter than the stool collection portion 31 in order to secure an air flow passage. On the other hand, the distal end side of the enlarged diameter portion 35 is tapered, and after the film 12a of the stocker 12 is broken, the enlarged diameter portion 35 closes the opening of the stocker 12 so that feces do not leak out.

  Note that the stool collection tube 10 is preferably formed of, for example, polypropylene or the like so as to withstand cryopreservation and centrifugation. Moreover, in order to improve the adhesiveness of the stool collection tube 10 and the filtrate tube 20, it is preferable to use low density polyethylene etc. which are different from the polypropylene of the stool collection tube 10 for the filtrate tube 20, for example. The stocker 12 is preferably formed of low density polyethylene or the like, and the stool collection rod 30 is preferably formed of ABS resin or the like.

  On the other hand, the filter 13 is a sintered body made of a synthetic resin such as polypropylene or polyethylene. Although the pore diameter varies depending on the target to be detected, it is preferable to use a filter having a pore size of about 100 to 2000 times the diameter of the virus particle. In addition, the diameter of a virus particle is about 20-300 nm in general. For example, when Norovirus is the virus to be collected in this collection method, the diameter of the virus particle is about 30 nm. Can be passed. On the other hand, since the size of bacteria is about 20 to 100 μm, it is captured by the filter. In addition, even if bacteria pass through the filter, it becomes a centrifugal sediment and does not affect the recovery of the virus suspension.

  Next, the handling method of this stool collection container in the fractionation work of a virus suspension is demonstrated.

  Before the stool collection, the empty filtrate tube 20 is attached to the lower end of the stool collection tube 10 packed with the buffer solution, and the stool collection rod 30 is removed in advance. When collecting the stool using the stool collection rod 30, the stool collection rod 30 to which the stool is attached is inserted from the upper end opening of the stool collection tube 10, and the membrane 12a of the stocker 12 is inserted at the tip 32 of the stool collection unit 31. Then, the stool collection portion 31 is inserted into the solution storage chamber 11, and the male thread portion 36 of the stool collection rod 30 is screwed into the female thread portion 14 of the stool collection tube 10 to be sealed (see FIG. 4).

  In order to separate the virus suspension, after thawing the frozen stool collection container and stirring it appropriately, the stool collection container 11 is directly placed in the lysate storage chamber 11 by directly centrifuging the stool collection container. The filter 13 installed at the lower end is used to remove food digestion residues and bacterial clumps in the stool and collect only the liquid layer in the empty filtrate tube 20. By applying a centrifugal force of preferably 1000 G or more, more preferably 2000 to 3000 G to the stool collection container in the centrifugal treatment, foreign matters including bacteria that have passed through the filter 13 are precipitated at the bottom of the filtrate tube 20. The virus mainly floats in the liquid layer of the filtrate tube 20. In addition, since a large centrifugal force of 1000 G or more is applied to the stool collection container, the suspension moves from the stool collection tube 10 to the filtrate tube 20 via the filter 13 even when the stool collection container is sealed.

  It should be noted that the screw between the stool collection tube 10 and the stool collection rod 30 (of the stool collection tube 10) immediately before being placed on the centrifuge so that the suspension smoothly moves from the stool collection tube 10 to the filtrate tube 20. Between the female threaded portion 14 and the male threaded portion 36 of the stool collection rod 30) and between the stool collection tube 10 and the filtrate tube 20 (between the male threaded portion 15 of the stool collection tube 10 and the female threaded portion 21 of the filtrate tube 20). The airtightness may be released by loosening. Since the male thread portion 15 of the stool collection tube 10 is screwed deeply into the filtrate tube 20, the suspension that has passed through the filter 13 does not leak out of the filtrate tube 20.

  After centrifugation, the filtrate tube 20 is preferably designed so that it can be removed from the stool collection tube 10 and transferred directly to the next step. For example, in this embodiment, the outer shape of the filtrate tube 20 conforms to the standard size of a 96-well microtiter plate of 8 rows and 12 columns. Match the pitch of the well. In the next step, the required amount of virus suspension can be dispensed using a standard 8 micropipette.

  As mentioned above, although the Example of this invention was described, this invention is not limited to said Example, In the range which does not deviate from the meaning, it can change suitably. For example, in the above embodiment, the stool collection portion has a quadrangular pyramid at the tip and a quadrangular prism at the tip, but may have a triangular pyramid at the tip and a triangular prism. Further, in the above embodiment, the recessed portions are provided alternately on the opposing surfaces of the stool collection unit, but may be provided on the same surface side. The point is that the desired function can be obtained in the present invention.

It is a longitudinal cross-sectional view of the container main body in one Example of the stool collection container which concerns on this invention. It is a side view of a stool collection stick in one example of a stool collection container concerning the present invention. (A) A direction arrow directional view in FIG. 2, (b) It is a BB arrow directional cross-sectional view in FIG. In one Example of the stool collection container which concerns on this invention, it is a longitudinal cross-sectional view which shows the state which inserted the stool collection stick | rod in the container main body.

Explanation of symbols

10 Stool Collection Tube 11 Solution Storage Chamber 12 Stocker (Sealing Member)
12a Membrane 12b Cylindrical part 12c Fin 12d Bottom part 12e Seal part 13 Filter 14, 21 Female thread part 15, 36 Male thread part 20 Filtrate tube 30 Stool collection rod 31 Stool collection part 32 Tip part 33 Shaft part 33a Recessed part 33b Rib 33c Both sides 34 Diameter reduction part 35 Diameter expansion part 37 Grasping part 40 Container body

Claims (7)

A dissolution chamber for storing a solution for dissolving stool is provided inside, a stool collection rod for collecting stool is inserted into one end, and a filter for filtering the suspension is provided at the other end. A method for collecting a virus suspension using a stool collection container characterized by comprising a tubular stool collection tube and a filtrate tube attached to the other end of the stool collection tube,
After inserting the stool collection stick attached to the stool collection container, the stool attached to the stool collection specimen was dissolved in the solution, and centrifugal force was applied to the stool collection container and passed through the filter. A method for collecting a virus suspension characterized by collecting only the liquid layer in the filtrate tube. The method for collecting a virus suspension according to claim 1, wherein the centrifugal force applied to the stool collection container is 1000 G or more. The method for collecting a virus suspension according to claim 1, wherein the centrifugal force applied to the stool collection container is 2000 to 3000G. The method for collecting a virus suspension according to any one of claims 1 to 3, wherein a stool collection container used in the method for collecting a virus suspension has the following characteristics (1) and (2).
(1) The solution storage chamber is a tubular stool collection tube whose one end is sealed with a breakable membrane.
(2) The stool collection rod is a rod-like member provided with a stool collection portion for collecting stool at the distal end side, the proximal end side of which is a gripping portion, and the stool collection portion is formed from a pyramid having a top portion on an axis. And a ridge line in which the pyramid and the prism are continuous. The stool collection part of the stool collection container used in the method for collecting the virus suspension has a concave part that accommodates feces in the shaft part, and a rib that divides the concave part is provided in the concave part. The method for collecting a virus according to any one of claims 1 to 4, wherein the virus is a stool. 6. The virus collection method according to claim 5, wherein in the virus suspension liquid collection method, both side portions of the shaft portion of the stool collection portion protrude toward the opening side of the recessed portion. In the method for collecting the virus suspension, a cylindrical sealing member is inserted into one end portion of the lysing solution storage chamber, and the breakable film is stretched on one end surface of the sealing member. A convex seal portion is provided around the outer peripheral portion of the member, and a plurality of fins are radially disposed above the seal portion. A method for collecting the virus suspension described in the above.