JP2006325517A - Cell releasing apparatus and scraping member package - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To efficiently release cells attached to the bottom surface of a culture vessel by a simple operation. <P>SOLUTION: The cell releasing apparatus 1 is equipped with a blade-like scraping member 5 that is inserted into the culture vessel 2 for culturing cells 4, etc., brought into contact with the bottom surface 3a of the culture vessel 2 and composed of an elastic body, a positioning means 6 for positioning the scraping member 5 to the culture vessel 2 and a rotation means 7 for rotating the scraping member 5 positioned to the culture vessel by the positioning means 6 around the normal line of the bottom surface 3a while keeping the scraping member in contact with the bottom surface 3a of the culture vessel 2. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention relates to a cell peeling device and a scraping member package.

Conventionally, a cell scraper has been used when cells and tissues are detached from the bottom of a culture container such as a petri dish without using a proteolytic enzyme such as trypsin (see, for example, Patent Document 1).
This cell scraper is composed of a blade made of a rubber-like elastic material, a scraping part made of its support, and a handle for holding it. In this cell scraper, an operator holds a handle, presses the blade of the scraping part against the bottom surface of the culture vessel, and rubs the blade over the entire bottom surface of the culture vessel, so that the grown cells adhere to the bottom surface of the culture vessel. The tissue is exfoliated.
Utility Model Registration No. 2531969

  However, the culture vessel is often circular like a petri dish, and if you try to peel off the cells adhering to such a circular bottom without waste, the blade can be made smaller and scraped to the corner of the culture vessel. It is necessary to. On the other hand, if the blade is made smaller, the amount of cells that can be detached by a single scraping operation is reduced, so that the operator needs to repeat the scraping operation, which is inconvenient.

  The present invention has been made in view of the above-described circumstances, and is a cell detaching apparatus that can efficiently and efficiently detach and recover cells adhering to the bottom surface of a culture vessel by a simple operation. The purpose is to provide.

In order to achieve the above object, the present invention provides the following means.
The present invention includes a blade-shaped scraping member made of an elastic body inserted into a culture container for culturing cells and the like and brought into contact with the bottom surface of the culture container, and positioning means for positioning the scraping member with respect to the culture container And a cell detaching device provided with a rotating means for rotating the scraping member positioned with respect to the culture vessel by the positioning means around the normal line of the bottom surface while maintaining the scraping member in contact with the bottom surface of the culture vessel. .

  According to the present invention, when the scraping member is inserted into the culture container in which the cells are attached to the bottom surface and cultured, and the scraping member is positioned on the culture container by the positioning means, the scraping member contacts the bottom surface of the culture container. Be made. Since the scraping member is formed of an elastic body, when it comes into contact with the culture vessel, it is elastically deformed and pressed with a predetermined pressure. By operating the rotating means in this state, the scraping member is rotated around the normal line of the bottom surface of the culture vessel, and the whole cell adhered to the bottom surface of the culture vessel is detached while being rotated once. It becomes possible to collect. Thereby, the cell can be easily detached from the culture vessel by a simple operation without having to reciprocate the cell scraper many times.

In the above invention, the positioning means may comprise a holder fixed to the upper end of the culture vessel while rotatably supporting the scraping member.
By doing in this way, a holder can be fixed to the upper end part of a culture container, a scraping member is rotated with respect to a holder, and a cell can be easily peeled from the whole bottom face of a culture container.

Further, in the above invention, the rotating means is disposed between an operating portion provided so as to be movable in a direction approaching and separating from the holder, and the operating portion and the scraping member. It is good also as providing the operation conversion means which converts translation operation into rotation operation of a scraping member.
In this way, when the operation unit is moved in the direction of approaching and separating from the holder, the translation operation of the operation unit is scraped off by the operation of the motion conversion means arranged between the operation unit and the scraping member. It is converted into a rotational motion of the member. Therefore, the operator can rotate the scraping member maintained in contact with the bottom surface of the culture vessel, and peel the cells from the bottom surface of the culture vessel simply by translating the operation unit.

In the above invention, the scraping member may be detachably attached to the holder.
By doing in this way, a scraping member can be attached to a holder at the time of use, a cell can be peeled off, and a scraping member can be removed from a holder after use. Accordingly, the scraping member can be made disposable, and a portion other than the scraping member can be reused, and a hygienic cell detaching device can be provided at a low cost.

In the above invention, a plurality of scraping members may be arranged at the same pitch as a plurality of wells provided in a culture vessel.
By doing so, it is possible to insert a scraping member into each of the plurality of wells and simultaneously perform a cell peeling operation.

Moreover, in the said invention, the said operation | movement conversion means is good also as converting the translation operation | movement of a single operation part into the rotation operation | movement of a some scraping member.
By doing so, the operator only has to translate the single operation unit, and the cells in the plurality of wells provided in the culture vessel can be easily detached at the same time.

In the above invention, the holder may be made of a transparent material.
By doing in this way, it can prevent that the visual field which observes the inside of a culture container is obstruct | occluded by a holder, and can perform peeling operation | work, confirming the peeling state of a cell.

Moreover, in the said invention, the said holder is good also as being formed in the lid shape which obstruct | occludes the opening part of a culture container.
By doing in this way, the opening part of a culture container can be obstruct | occluded with a holder in the case of peeling operation | work. Therefore, it is possible to prevent the cells detached in the cell peeling operation from leaking outside from the opening of the culture container.

In addition, the present invention is a scraping member package for any one of the above-described cell detaching apparatuses, wherein the plurality of scraping members are accommodated in a state in which a plurality of scraping members are arranged at the same pitch as the wells of the culture vessel. A scraper package is provided.
According to the present invention, since the plurality of scraping members are provided in an arrangement state preliminarily aligned with the well pitch of the culture vessel, there is no need to mount them individually when mounting on the holder, and the scraping members can be easily scraped off. The member can be attached without being directly operated by hand.

  According to the present invention, there is an effect that cells that adhere and grow to the bottom surface of the culture vessel can be efficiently detached and recovered without waste by a simple operation.

A cell peeling apparatus according to an embodiment of the present invention will be described below with reference to FIGS.
The cell peeling apparatus 1 according to the present embodiment is configured so that cells 4 cultured in a circular accommodation recess (well) 3 of a culture container 2 such as a petri dish can be obtained by using the bottom surface of the accommodation recess 3 without using a proteolytic enzyme such as trypsin. As shown in FIG. 1, the scraping member 5 inserted into the housing recess 3, the holder 6 for positioning the scraping member 5 in the housing recess 3, and the scraping member 5. And rotating means 7 for rotating the.

  The scraping member 5 is a plate-like member made of an elastic body such as rubber, and is provided with a blade surface 5a that is in close contact with the bottom surface 3a of the housing recess 3 of the culture vessel 2 on one side thereof. The blade surface 5a is pressed against the bottom surface 3a of the housing recess 3 with a predetermined pressing force, and is moved in a direction along the bottom surface 3a. It can be made to peel from. Further, the scraping member 5 has a length that is slightly longer than the radial dimension of the bottom surface 3 a of the housing recess 3.

The scraping member 5 is maintained in a flat plate shape by being fixed to the support portion 8 opposite to the blade surface 5a in the width direction. The support portion 8 is configured integrally with the holder 6.
The holder 6 constitutes a disc-shaped lid that is fitted into the upper end opening 3b of the housing recess 3 of the culture vessel 2 and closes the opening 3b. The holder 6 is provided with a stepped portion 6 a that abuts against the upper end surface 2 a of the culture vessel 2 when the holder 6 is fitted into the upper end opening 3 b of the accommodating recess 3. The stepped portion 6a is such that the blade surface 5a of the scraping member 5 comes into contact with the bottom surface 3a of the housing recess 3 in a state where a predetermined pressing force is generated when it hits the upper end surface 2a of the culture vessel 2. They are arranged in a positional relationship with respect to 5a.

  The holder 6 is preferably made of a transparent material. By making the holder 6 transparent, even if the holder 6 is disposed at a position where the upper end opening 3b of the receiving recess 3 is closed, the state in the receiving recess 3 can be observed from the outside, and the state of the cells is observed. There is an advantage that the peeling operation can be performed.

  The rotating means 7 is a handle provided on the other surface of the holder 6 opposite to the support portion 8, and the holder 6 directs the support portion 8 toward the inside of the accommodating recess 3 of the culture vessel 2. In addition, a rotational force is applied by an operator in a state of being fitted into the upper end opening 3 b of the housing recess 3. As a result, the holder 6 can be rotated around the normal passing through the center of the bottom surface 3a of the receiving recess 3 with the inner surface of the receiving recess 3 fitted as a guide.

The operation of the cell peeling apparatus 1 according to the present embodiment configured as described above will be described below.
In order to peel the cells 4 in the housing recess 3 using the cell peeling device 1 according to the present embodiment, as shown in FIG. 2A, the blade surface 5a of the scraping member 5 attached to the holder 6 is used. Is inserted into the housing recess 3 and fitted into the upper end opening 3 b of the housing recess 3. Thereby, the holder 6 functions as a lid, and the upper end opening 3b of the housing recess 3 is closed. Then, the blade surface 5 a of the scraping member 5 supported by the support portion 8 of the holder 6 is fitted into the housing recess 3 by fitting the step portion 6 a of the holder 6 to a position where it abuts against the upper end surface 2 a of the culture vessel 2. The bottom surface 3a is brought into contact with a predetermined pressing force.

  In this state, as shown in FIGS. 2 (b) and 3, the operator holds the handle as the rotating means 7 provided in the holder 6 and rotates the holder 6 with respect to the culture vessel 2. As a result, the scraping member 5 inserted into the housing recess 3 of the culture vessel 2 is rotated so that the bottom surface 3a of the housing recess 3 is scraped off by the blade surface 5a. At this time, the blade surface 5a of the scraping member 5 inserted into the housing recess 3 is accommodated by rotating the step 6a of the holder 6 while maintaining a state where it abuts against the upper end surface 2a of the culture vessel 2. The cells 4 that are moved while being pressed against the bottom surface 3a of the concave portion 3 with a predetermined pressing force and adhere to the bottom surface 3a can be scraped.

  In the present embodiment, the scraping member 5 has a length that is slightly longer than the radial dimension of the bottom surface 3 a of the housing recess 3, so that it is rotated 360 ° with respect to the bottom surface 3 a of the housing recess 3. Thus, the cells 4 adhered to the bottom surface 3a can be peeled without leaving them. Therefore, as compared with the conventional method in which the cells 4 are peeled off by rubbing with a small cell scraper many times, the cells 4 can be peeled off very easily and without waste. Cells can be recovered.

  Further, when the culture container 2 is provided with a plurality of receiving recesses 3 and the cells 4 are cultured in each receiving recess 3, the cells 4 in one receiving recess 3 are peeled off by the cell peeling device 1 of the present embodiment. Thereafter, the cell peeling device 1 is transferred to the other housing recess 3 and the cells 4 in the other housing recess 3 can be peeled off without waste by the same operation.

Next, the cell peeling apparatus 10 which concerns on the 2nd Embodiment of this invention is demonstrated below with reference to FIGS.
As shown in FIG. 4, the cell peeling apparatus 10 according to the present embodiment includes a scraping member 11 inserted into the culture vessel 2, a support member 12 that supports the scraping member 11, and the scraping member 11. A holder 13 that is in contact with the upper end surface 2a of the culture vessel 2 while being inserted into the housing recess 3 of the culture vessel 2 and fixed relative to the culture vessel 2, and a scraping member 11 provided on the holder 13 are provided. And a rotating mechanism 14 for rotating.

  The scraping member 11 is made of a flat elastic body similar to that of the first embodiment, and has a straight blade surface 11a on one surface thereof. The blade surface 11a has a length dimension substantially equal to the diameter dimension of the bottom surface 3a of the accommodating recess 3 of the culture vessel 2.

  The support member 12 includes a disk-like support portion 15 that supports the scraping member 11 and a shaft portion 16 that extends perpendicularly to the support portion 15 from the center position of the support portion 15. The shaft portion 16 has an outer diameter that is inserted into a central hole 17 of the holder 13 to be described later with a gap, and is rotatably inserted into the central hole 17 of the holder 13.

  The shaft portion 16 includes a step portion 16 a that abuts the holder 13 when inserted into the central hole 17 of the holder 13, and an engagement piece 16 b that engages the holder 13 to prevent the holder 13 from falling off. It has been. The engaging piece 16b is a leaf spring-like member that protrudes obliquely from the outer surface of the shaft portion 16, and is provided so as to be able to advance and retract in the radial direction by elastic deformation, and in the state of protruding outward in the radial direction, the center of the holder 13 It protrudes larger than the inner diameter dimension of the hole 17 and engages with the holder 13 so that it can pass through the central hole 17 of the holder 13 while being retracted radially inward.

  The holder 13 includes a flat plate portion 13a having the central hole 17 and leg portions 13b extending from the flat plate portion 13a in the thickness direction of the flat plate portion 13a. The flat plate portion 13a has a thickness dimension that is sandwiched between the step portion 16a and the engaging piece 16b when the shaft portion 16 passes through the central hole 17 and abuts the step portion 16a. Thus, the displacement along the axial direction of the shaft portion 16 can be restricted.

  The holder 13 is provided with two through holes 18 parallel to the central hole 17, and operation rods 19 are respectively inserted into the through holes 18. One end of the two operating rods 19 is connected to the handle 20. A coil spring 21 is fitted into the operation rod 19 arranged between the handle 20 and the holder 13. Thereby, the handle 20 is always urged in a direction away from the holder 13.

  Further, a cylindrical portion 22 is provided at the center of the handle 20 for fitting one end of the shaft portion 16 in a state of being brought close to the holder 13. The inner diameter dimension of the cylindrical portion 22 is set to be equal to or less than the inner diameter dimension of the central hole 17 of the holder 13.

  The rotation mechanism 14 includes a roller 24 fixed to a rotary shaft 23 extending radially inward of the leg portion 13b, a pinion gear 25 fixed to the roller 24, and a rack gear provided at the tip of the operation rod 19. 26. The roller 24 is, for example, a rubber roller, and is pressed against the surface of the support portion 15 of the support member 12 in a state where the shaft portion 16 is inserted into the central hole 17 of the holder 13 and the step portion 16a and the holder 13 are in contact with each other. Can be contacted. The roller 24 is disposed at a position where the rack gear 26 at the tip of the operation rod 19 that has passed through the through hole 18 of the holder 13 meshes with the pinion gear 25 of the roller 24.

  A connecting portion 27 made of an elastic body is provided at the tip of the leg portion 13b. As shown in FIG. 5, the length of the leg portion 13 b is such that when the scraping member 11 is brought into contact with the bottom surface 3 a of the housing recess 3 of the culture vessel 2 with a predetermined pressing force, the connecting portion 27 is placed in the culture vessel. 2 has such a size that it is arranged in a pressed state on the upper end surface 2a.

The operation of the cell peeling apparatus 10 according to the present embodiment configured as described above will be described below.
In order to peel off the cells 4 adhered to the bottom surface 3a of the housing recess 3 of the culture vessel 2 using the cell peeling device 10 according to this embodiment, the scraping member 11 is supported as shown in FIG. The shaft portion 16 of the support member 12 is inserted into the central hole 17 of the holder 13, and the flat plate portion 13a of the holder 13 is sandwiched between the step portion 16a of the shaft portion 16 and the engagement piece 16b as shown in FIG. Make it. On the other hand, the operation rod 19 is inserted into each of the two through holes 18 of the holder 13, and the rack gear 26 at the tip thereof is meshed with the pinion gear 25 of the roller 24 provided rotatably on the leg portion 13 b of the holder 13. .

  As shown in FIG. 5, the scraping member 11 of the cell peeling device 10 of the present embodiment configured as described above is inserted into the accommodating recess 3 of the culture vessel 2 and the blade surface 11a at the tip of the scraping member 11 is inserted. In close contact with the bottom of the culture vessel. At this time, the connection portion 27 of the holder 13 is brought into contact with the upper end surface 2a of the culture vessel 2 in a pressed state with a predetermined pressure, and the holder 13 is fixed so as not to move with respect to the culture vessel 2 due to friction of the connection portion 27. The

  In this state, the handle 20 is pushed down as shown by an arrow A as shown in FIG. As a result, the operation rod 19 fixed to the handle 20 is also pushed down integrally while elastically deforming the coil spring 21, so that the rack gear 26 at the tip of the operation rod 19 is moved in the longitudinal direction and meshed with the rack gear 26. The pinion gear 25 is rotated around the rotation shaft 23. Since the pinion gear 25 is fixed to the roller 24, the roller 24 rotates, and the support portion 15 in contact with the outer peripheral surface of the roller 24 is rotated around the axis of the shaft portion 16. As a result, the scraping member 11 fixed to the support portion 15 is rotated inside the accommodating recess 3 of the culture vessel 2 as shown by an arrow B in FIG. It is made to peel from 3a.

  In this case, since the blade surface 11 has a length dimension equivalent to the diameter of the bottom surface 3a of the housing recess 3 of the culture vessel 2, the blade surface 11a is simply rotated by 180 °. The entire bottom surface 3a of the housing recess 3 of the culture vessel 2 is scraped, and the cells 4 are peeled off without waste.

  Since the coil spring 21 is disposed between the handle 20 and the holder 13, when the handle 20 is moved in the direction close to the holder 13, the coil spring 21 is compressed and attached to the direction in which the handle 20 is pushed back. To force. Thereby, the handle 20 can be pushed back in the direction away from the holder 13 by releasing the pressing force to the handle 20.

  Therefore, the operator applies a pressing force to the handle 20 intermittently, thereby reciprocating the handle 20 with respect to the holder 13 in the direction of approaching and separating, so that the scraping member 11 can be easily moved in the housing recess 3 of the culture vessel 2. Can be rotated back and forth. As a result, the cells 4 can be efficiently detached with little effort.

  Further, after the peeling operation is completed, as shown in FIG. 7, by pressing the handle 20 so as to be closest to the holder 13, the shaft portion 16 is inserted into the cylindrical portion 22 provided in the handle 20. Insert the tip. Thereby, the engaging piece 16b provided in the shaft part 16 is elastically deformed so as to be retracted in the radial direction. Since the inner diameter dimension of the cylindrical portion 22 is set to be equal to or smaller than the inner diameter dimension of the central hole 17 of the holder 13, the engagement piece 16 b is contracted radially inward to a dimension that can pass through the central hole 17. . Thereby, the scraping member 11 and the support member 12 can be removed from the holder 13.

  That is, after the work of detaching the cells 4 in the culture container 2 is completed, the used scraping member 11 can be replaced with a new scraping member 11 by removing the scraping member 11 from the holder 13. By exchanging only the scraping member 11 that contacts the cells 4, it is possible to prevent the occurrence of cross-contamination between different cells 4. Further, by making the scraping member 11 disposable, it becomes possible to reuse other parts, and to reduce the cost.

  In the present embodiment, the operation conversion means for converting the translational operation of the operation rod 19 into the rotation operation of the scraping member 11 by the meshing of the rack gear 26 provided on the operation rod 19 and the pinion gear 25 provided on the roller 24 is provided. Although illustrated, instead of this, other motion conversion means 28 as shown in FIG. 8 may be adopted. The motion converting means 28 rotatably connects the tip of the operating rod 30 to a position eccentric from the center of the wheel 29 attached to the roller 24, and rotatably connects the other end of the operating rod 30 to the tip of the lever 31. Thus, a lever crank mechanism is configured.

The lever 31 is provided with a leaf spring 32 that urges the tip to push down. For example, when the operator grasps or releases the lever 31, the lever 31 is reciprocally swung, thereby the wheel 29. And the roller 24 is rotated, and the scraping member 11 in contact with the roller 24 can be rotated as shown by an arrow.
In FIG. 8 and FIG. 9, reference numeral 32 denotes an attachment portion comprising a dovetail groove 32a and an ant tenon 32b for detachably attaching the scraping member 11 to the support portion 15.

  In the present embodiment, the case has been described in which the scraping member 11 is inserted into the single housing recess 3 to separate the cells 4, but the culture vessel 2 is provided with a plurality of housing recesses 3, each housing When the cells 4 are cultured in the recess 3, it is preferable to use a cell peeling device 33 in which a plurality of scraping members 11 are attached to a holder 13 'as shown in FIG. This cell peeling device 33 is different from that shown in FIG. 4 in that a single holder 13 ′ is used for a plurality of scraping members 11 and that all the operating rods 19 are connected to a single handle 20 ′. This is different from the cell peeling apparatus 10 of FIG. By doing so, the plurality of scraping members 11 are rotated in the plurality of receiving recesses 3 of the culture vessel 2 only by moving the single handle 20 ′ in the vertical direction, and the plurality of receiving recesses 3 at a time. The inner cell 4 can be detached.

  Moreover, it is complicated to replace the scraping member 11 one by one when the scraping member 11 is replaced with the cell peeling device 33 having the plurality of scraping members 11 as described above. It is preferable to be able to When removing the scraping member 11 from the holder 13 ′, as described above, the handle 20 is pushed down, so that each engagement piece 16 b is pushed inward in the radial direction by the cylindrical portion 22 provided on the handle 20. It can be removed at a time so that it can pass through the hole 17.

  Further, in order to attach the plurality of scraping members 11 to the holder 13 ′ at a time, the plurality of scraping members are directed in the same direction according to the arrangement pitch of the central holes 17 provided in the holder 13 ′. 11 must be arranged. In this case, as shown in FIGS. 11 and 12, the scraping member 11 is placed in a case 35 having a plurality of receiving portions 34 for arranging the plurality of scraping members 11 in accordance with the arrangement pitch of the central holes 17. It is preferable to accommodate the supporting member 12 provided. By providing the upper opening of the case 35 as a scraping member package 37 closed in a sealed state by the cover 36, the cover 36 can be opened at the time of replacement, and the plurality of scraping members 11 can be replaced at a time. Also, it is possible to provide the scraping member 11 in a clean state in which bacteria and dust are not attached.

It is a front view which shows the cell peeling apparatus which concerns on the 1st Embodiment of this invention. It is a figure which shows the state which inserted the scraping member of the cell peeling apparatus of FIG. 1 in the accommodation recessed part of a culture container, (a) The front view which fractured | ruptured one part, (b) Relationship between a scraping member and an accommodation recessed part FIG. FIG. 3 is a plan view of FIG. It is a longitudinal cross-sectional view which shows the cell peeling apparatus which concerns on the 2nd Embodiment of this invention. It is a longitudinal cross-sectional view which shows the state which inserted the scraping member of the cell peeling apparatus of FIG. 4 in the accommodation recessed part of a culture container. It is a longitudinal cross-sectional view which shows the state from which the scraping member of the cell peeling apparatus of FIG. 4 was removed. It is a longitudinal cross-sectional view which shows the state at the time of removing the scraping member of the cell peeling apparatus of FIG. It is a longitudinal cross-sectional view which shows the modification of the cell peeling apparatus of FIG. It is a partial front view explaining the attachment structure of the scraping member of the cell peeling apparatus of FIG. FIG. 5 is a front view showing another modification of the cell peeling device of FIG. 4 and showing a cell peeling device having a plurality of scraping members. It is a top view which shows the scraping member package for the cell peeling apparatus of FIG. It is a longitudinal cross-sectional view of the scraping member package of FIG.

Explanation of symbols

1,10,33 Cell detachment device 2 Culture vessel 3 Well (accommodation recess)
3a Bottom 3b Upper end opening (opening)
4 cell 5,11 scraping member 6,13,13 'holder (positioning means)
7 Handle (Rotating means)
14 Rotating mechanism (rotating means)
20 Handle (operation part)
25 Pinion gear (motion conversion means)
26 Rack gear (motion conversion means)
29 wheel (motion conversion means)
30 Operation rod (motion conversion means)
37 Scraping material package

Claims (9)

A blade-shaped scraping member made of an elastic body that is inserted into a culture container for culturing cells and the like and brought into contact with the bottom surface of the culture container;
Positioning means for positioning the scraping member with respect to the culture vessel;
A cell detaching apparatus comprising: a rotating unit that rotates the scraping member positioned with respect to the culture vessel by the positioning unit around the normal line of the bottom surface while maintaining the scraping member in contact with the bottom surface of the culture vessel.   The cell peeling apparatus according to claim 1, wherein the positioning means includes a holder that rotatably supports the scraping member and is fixed to an upper end portion of the culture vessel.   The rotating means is disposed between an operating portion movably provided in a direction approaching and separating from the holder, and between the operating portion and the scraping member, and the translation operation of the operating portion is controlled by the scraping member. The cell peeling apparatus according to claim 2, further comprising a motion conversion unit that converts the rotational motion.   The cell peeling apparatus according to claim 2 or 3, wherein the scraping member is detachably attached to the holder.   The cell scraping device according to any one of claims 1 to 4, wherein a plurality of the scraping members are arranged at the same pitch as the plurality of wells provided in the culture vessel.   The cell detachment apparatus according to claim 5, wherein the motion conversion means converts a translation operation of a single operation unit into a rotation operation of a plurality of scraping members.   The cell peeling device according to any one of claims 2 to 6, wherein the holder is made of a transparent material.   The cell peeling device according to any one of claims 2 to 7, wherein the holder is formed in a lid shape that closes an opening of a culture vessel. A scraping member package for the cell detaching device according to claim 5,
A scraping member package in which the plurality of scraping members are accommodated in a state where a plurality of scraping members are arranged at the same pitch as the wells of the culture vessel.

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