JP2006300721A - Thermal lens spectrometric analytical system, and thermal lens signal correction method - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a thermal lens spectrometric analytical system and a thermal lens signal correction method, which measure accurately a sample, even when thermal lens signal intensity is varied by an external environment change. <P>SOLUTION: This thermal lens spectrometric analytical system 10 is provided with: a micro chemical chip 2 having a groove 1 injected with the in-liquid sample in an inside; a refractive index distribution type rod lens 3 for converging excitation light and detection light propagated from a light source unit 7 via an optical fiber 5 to the liquid sample to generate a thermal lens image; a photoelectric converter 22 for detecting light quantities of the excitation light and the detection light, and the thermal lens signal intensity; and a personal computer 25 for correcting a measured value of the thermal lens signal intensity, by multiplying the measured value of the thermal lens signal intensity with the first ratio (prescribed light quantity of the excitation light/measured light quantity of the excitation light), and/or the second ratio (prescribed light quantity of the detection light/measured light quantity of the detection light). <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention relates to a thermal lens spectroscopic analysis system and a thermal lens signal correction method.

Conventionally, integration technology for performing chemical reactions in a minute space has attracted attention from the viewpoint of speeding up chemical reactions, reactions in minute amounts, on-site analysis, etc. Yes.

One such integration technique is the use of microchemical chips to mix liquid samples,
There is a thermal lens spectroscopic analysis system that performs reaction, separation, extraction, detection, and the like.

  For example, as shown in FIG. 4, the thermal lens spectroscopic analysis system 1000 is disposed above the plate member 120 with a channel and the plate member 120 with a channel in which a sample in liquid is filled in the channel. The optical fiber 100 with a lens having a lens attached to the tip, and the sample in liquid in the flow path of the plate-like member 120 with the flow path connected to the optical fiber 100 with the lens are irradiated with the excitation light, and the irradiated excitation light The light source unit 110 that irradiates the thermal lens generated in the sample in the liquid with the detection light and the plate member 120 with the flow path are disposed below the flow path of the plate member 120 with the flow path by the excitation light. And a detection device 130 that detects detection light through a thermal lens generated in the sample in liquid.

  The optical fiber 100 with a lens includes a lens 102, an optical fiber 101 having one end connected to the lens 102 and the other end connected to the light source unit 110, and a sleeve 104 that fixes the optical fiber 101 via a ferrule 103.

The light source unit 110 includes an excitation light source 105 that outputs excitation light, and an excitation light source 105.
And a modulator 107 that modulates excitation light output from the excitation light source 105, a detection light source 106 that outputs detection light, and an optical fiber 114 for the excitation light source 105 and the detection light source 106, respectively. The excitation light output from the excitation light source 105 and the detection light output from the detection light source 106 and combined into the optical fiber 101. It comprises an optical multiplexer 108 on which these excitation light and detection light are respectively incident.

  The plate member 120 with a flow path is composed of an upper glass substrate 201, a middle glass substrate 202, and a lower glass substrate 203 which are bonded in three layers in order from the optical fiber 100 with lens. The middle glass substrate 202, which is an intermediate layer of the plate member 120 with a flow path, is submerged in the liquid during operations such as mixing, stirring, synthesis, separation, extraction, and detection of the submerged sample by the thermal lens spectroscopic analysis system 1000. A flow path 204 through which a sample flows is provided.

The detection device 130 is disposed at a position facing the flow path 204 of the plate member 120 with flow path and facing the optical fiber 100 with a lens, and separates the combined excitation light and detection light. A wavelength filter 402 that selectively transmits only detection light; a photoelectric converter 401 for detecting detection light disposed below the wavelength filter 402 and facing the flow path 204; The computer 405 is connected to the converter 401 via a lock-in amplifier 404 (see, for example, Patent Document 1).

  In the thermal lens spectroscopic analysis system 1000, the light amounts of the excitation light and the detection light output from the excitation light source 105 and the detection light source 106 are changed by the influence of changes in the external environment such as a temperature change. Due to a change in the loss of the waver 108, expansion of a stage (not shown) on which the thermal lens spectroscopic analysis system 1000 is mounted, or the like, the positional deviation between the lens 102 and the plate member 120 with a flow path or the lens 102 and the photoelectric converter 401 are misaligned.

  As a result, the amount of excitation light incident on the sample in the liquid in the flow path 204 to form the thermal lens may change, or the amount of detection light incident on the photoelectric converter 401 may change. is there.

  In addition, since the thermal lens spectroscopic analysis method executed by the thermal lens spectroscopic analysis system 1000 is usually analog measurement, the change in the amount of excitation light incident on the liquid sample in the channel 204 and the photoelectric converter described above. The change in the amount of the detection light incident on 401 appears as a change in the intensity of the thermal lens signal finally measured, and the reproducibility in the measurement of the thermal lens signal intensity is reduced.

  In order to improve the reproducibility in the thermal lens signal intensity measurement, an electronic component such as a Peltier element is added to the light source unit 110 to control the temperature, or the measurement environment of the thermal lens spectroscopic analysis system 1000 is strictly controlled. It is possible to remove external environmental changes such as temperature changes that are the root cause of the reproducibility degradation in thermal lens signal intensity measurement, but in this case, thermal lens spectroscopic analysis by adding electronic parts such as Peltier elements There are problems such as an increase in the size and cost of the system 1000 and a lack of versatility in the measurement environment.

Therefore, it is conceivable to correct the thermal lens signal intensity in accordance with the change in the amount of excitation light incident on the liquid sample in the flow path 204 and the change in the amount of detection light incident on the photoelectric converter 401. There has already been disclosed a radiant heat sensor that uses two photo-sensitive detectors to correct the amount of laser light measured (see, for example, Patent Document 2). In a spatial optical system that does not use an optical fiber such as this radiant heat sensor, the light can be branched by a filter or the like installed in the middle of the optical path, so the amount of laser light is easily corrected based on the amount of the branched light. be able to.
JP 2002-365252 A US Pat. No. 5,513,006

  However, in the thermal lens spectroscopic analysis system using an optical fiber, since light is confined in the optical fiber, a special branch module is required to branch the light, and the branched light cannot be easily detected. In particular, many branch modules that branch light in the visible region are easily affected by temperature changes, and the light branch ratio varies. Therefore, in the branch light detection for correcting the thermal lens signal intensity, it is not possible to distinguish whether the change in the light branching ratio is detected or the change in the light quantity of the excitation light and the detection light is detected. .

  In addition, since the thermal lens spectroscopic analysis system uses two types of light, excitation light and detection light, at least two detectors are required to detect the amount of excitation light and detection light. The analysis system becomes large.

  In addition, in order to correct the loss of the optical multiplexer 108, it is necessary to detect the amount of light after the excitation light and the detection light are combined, but the excitation light and the detection light combined in the optical fiber are separated and detected. Difficult to do.

  An object of the present invention is to provide a small thermal lens spectroscopic analysis system and a thermal lens signal correction method capable of accurately measuring a sample even when the thermal lens signal intensity changes due to a change in the external environment.

  In order to achieve the above object, the thermal lens spectroscopic analysis system according to claim 1 includes a chip having a groove into which a liquid sample is injected, and excitation that is propagated to the liquid sample from a light source through an optical transmission path. In a thermal lens spectroscopic analysis system including an objective lens that collects light and detection light to generate a thermal lens signal, a light amount measurement unit that measures the light amount of the excitation light and the detection light, and the intensity of the thermal lens signal A thermal lens signal intensity measuring unit that measures the first ratio of the predetermined light amount of the excitation light and the measured light amount of the excitation light, and the predetermined light amount of the detection light and the light amount of the measured detection light A ratio calculation unit that calculates the second ratio of the measured thermal lens signal by integrating the intensity of the measured thermal lens signal, the first ratio, and / or the second ratio. Thermal lens to correct intensity Characterized in that it comprises a No. intensity detection correction unit.

  The thermal lens spectroscopic analysis system according to claim 2 is characterized in that, in the thermal lens spectroscopic analysis system according to claim 1, the light quantity measuring unit for measuring the light quantity of the excitation light and the detection light comprises a single detector. To do.

  The thermal lens spectroscopic analysis system according to claim 3 is the thermal lens spectroscopic analysis system according to claim 1, and measures the intensity of the thermal lens signal and a light quantity measuring unit that measures the light quantity of the excitation light and the detection light. The thermal lens signal intensity measuring unit is characterized by comprising one detector.

  The thermal lens spectroscopic analysis system according to claim 4 is the thermal lens spectroscopic analysis system according to claim 2 or 3, wherein an excitation light transmission filter that transmits only the excitation light and a detection light transmission filter that transmits only the detection light. A light transmissive filter installed so as to be interchangeable is provided, and the detector measures the amount of excitation light and detection light transmitted through the light transmissive filter.

  The thermal lens spectroscopic analysis system according to claim 5 is the thermal lens spectroscopic analysis system according to any one of claims 1 to 4, wherein the intensity of the thermal lens signal is corrected based on an output value of the detector. It is characterized by that.

  The thermal lens spectral analysis system according to claim 6 is the thermal lens spectral analysis system according to claim 5, wherein the output value of the detector is a current value.

  The thermal lens spectral analysis system according to claim 7 is the thermal lens spectral analysis system according to claim 5, wherein the output value of the detector is a voltage value.

  The thermal lens spectroscopic analysis system according to claim 8 is the thermal lens spectroscopic analysis system according to claim 7, further comprising an audio input terminal for measuring the voltage value.

  The thermal lens spectroscopic analysis system according to claim 9 is the thermal lens spectroscopic analysis system according to any one of claims 1 to 8, wherein the light transmission path is an optical fiber.

  The thermal lens spectroscopic analysis system according to claim 10 is the thermal lens spectroscopic analysis system according to any one of claims 1 to 9, wherein the objective lens is a rod lens.

  The thermal lens spectral analysis system according to claim 11 is the thermal lens spectral analysis system according to any one of claims 1 to 10, wherein the thermal lens signal is obtained by a fast Fourier transform process.

  13. The thermal lens signal correction method according to claim 12, wherein the thermal lens signal correction method corrects a thermal lens signal generated by irradiating the liquid sample injected into the groove in the chip with excitation light and detection light. A light quantity measuring step for measuring the light quantity of the light and the detection light; a thermal lens signal intensity measuring step for measuring the intensity of the thermal lens signal; and a predetermined light quantity of the excitation light and a light quantity of the measured excitation light. A ratio calculating step of calculating a ratio of 1 and a second ratio between the predetermined light amount of the detection light and the measured light amount of the detection light, the intensity of the measured thermal lens signal, the first ratio, And / or a thermal lens signal intensity detection / correction step of correcting the intensity of the measured thermal lens signal by integrating the second ratio.

  According to the thermal lens spectroscopic analysis system of claim 1 and the thermal lens signal correction method of claim 12, the intensity of the measured thermal lens signal, the predetermined amount of excitation light and the measured amount of excitation light The intensity of the thermal lens signal measured by correcting the ratio of 1 and the second ratio of the predetermined light quantity of the detected light and the measured light quantity of the detected light is corrected. Even if it changes, the sample can be measured accurately.

  According to the thermal lens spectroscopic analysis system of claim 2, since the light quantity of the excitation light and the light quantity of the detection light used for correcting the intensity of the measured thermal lens signal are measured by one detector, the thermal lens The spectroscopic analysis system can be miniaturized.

  According to the thermal lens spectroscopic analysis system of claim 3, since the intensity of the thermal lens signal is measured by one detector in addition to the light amount of the excitation light and the light amount of the detection light, the structure of the thermal lens spectroscopic analysis system is It can be simplified and further downsized.

  According to the thermal lens spectroscopic analysis system of claim 4, since the mechanism is to measure the light quantity of the excitation light and the light quantity of the detection light by replacing the filter that transmits only the excitation light and the filter that transmits only the detection light, The thermal lens spectroscopic analysis system can be further miniaturized by simplifying the structure of the vessel, and the thermal lens signal can be reliably corrected by reliably measuring changes in the amount of excitation light and detection light.

  Hereinafter, embodiments of the present invention will be described in detail with reference to the drawings.

  FIG. 1 is a diagram schematically showing a configuration of a thermal lens spectroscopic analysis system according to an embodiment of the present invention.

  In FIG. 1, a thermal lens spectroscopic analysis system 10 is disposed on a microchemical chip 2 having a groove 1 into which a sample in liquid is injected, and on the microchemical chip 2 above the groove 1 with a predetermined interval. A refractive index distribution type rod lens 3 such as a cylindrical SELFOC (registered trademark) that condenses light propagated from an optical fiber 5 described later in the groove 1 to generate a thermal lens signal, and a refractive index distribution type rod lens 3 The single-mode optical fiber 5 for visible light that propagates light to the gradient index rod lens 3, the ferrule 4 that holds the optical fiber 5, and the gradient index rod lens 3 are fixed and the optical fiber. 5 is connected to the optical fiber 5 through the ferrule 4 and the sleeve 6 is fixed in the liquid in the groove 1 of the microchemical chip 2 via the optical fiber 5. And a light source unit 7 for irradiating detection light to a thermal lens generated in a sample in the liquid by the irradiated excitation light, and a light source unit 7 disposed below the microchemical chip 2. And a detection device 8 that detects the detection light through a thermal lens generated in the sample in the liquid in the groove 1 of the microchemical chip 2 by the irradiated excitation light.

The microchemical chip 2 has a groove 1 through which a submerged sample flows when operations such as mixing, stirring, synthesis, separation, extraction, and detection of the submerged sample are performed by the thermal lens spectroscopic analysis system 10.

The material of the microchemical chip 2 is desirably glass in terms of durability and chemical resistance.
Considering the use of biological samples such as cells, for example for DNA analysis, glass having high acid resistance and alkali resistance, specifically, borosilicate glass, soda lime glass, aluminoborosilicate glass, and quartz glass are preferable. . However, organic substances such as plastics can be used by limiting the application.

The light source unit 7 is connected to the excitation light source 14 that outputs the excitation light, and the excitation light source 14 that is output from the excitation light source 14, for example, emits excitation light having a wavelength of 658 nm, for example, with a period of 1 kHz. A modulator 15 that modulates the light source, a detection light source 16 that outputs detection light having a wavelength of 785 nm, for example, an excitation light source 14 and a detection light source 16 via optical fibers 17 and 18, respectively. The excitation light output from the excitation light source 14 and the detection light output from the detection light source 16 connected to the optical fiber 5 are combined, and the excitation light and detection light that are combined in the optical fiber 5 are incident respectively. And a multiplexer 19 to be used.

In the light source unit 7, the excitation light output from the excitation light source 14 and the detection light output from the detection light source 16 are combined using a dichroic mirror instead of the multiplexer 19, and combined with the optical fiber 5. These excited excitation light and detection light may be incident.

  The detection device 8 is disposed at a position facing the transmission member 20 in which a pinhole 20 a that transmits only a part of light and the groove 1 of the microchemical chip 2 is opposed to the optical fiber 5. A filter 21 that separates and selectively transmits the combined excitation light and detection light, and is disposed at a position below the filter 21 and facing the groove 1, and the amount of excitation light and detection light And a photoelectric converter (silicon photodiode) 22 (detector) for detecting the thermal lens signal intensity, and connected to the photoelectric converter 22 via an IV amplifier (current-voltage conversion amplifier) 23 and a voltmeter 24. And a personal computer (PC) 25 (thermal lens signal intensity detection correction unit). The voltmeter 24 may be inserted between the IV amplifier 23 and the PC 25 or may be connected to the IV amplifier 23 independently. In this case, the IV amplifier 23 and the PC 25 are directly connected. The signal obtained from the photoelectric converter 22 is then analyzed in the PC 25.

  FIG. 2 is a diagram schematically showing the configuration of the detection apparatus in FIG.

  In FIG. 2, the filter 21 has a bandwidth of 20 nm for transmitting only excitation light having a wavelength of 658 nm and a bandpass filter for excitation light having a diameter of 12.5 mmφ, a bandwidth of 20 nm for transmitting only detection light having a wavelength of 785 nm, and a diameter of 12 .5 mmφ detection light bandpass filter 31, and these bandpass filters 30, 31 are configured to be interchangeable by a left-right replacement system, a rotation system, a sampling system, or the like.

  The measurement of the thermal lens signal intensity is performed with the output of the IV amplifier 23 in a state where the detection light band-pass filter 31 is disposed at a position facing the pinhole 20a formed in the transmission member 20 (FIG. 2). This is performed by inputting a signal to the PC 25 via the voltmeter 24. The output signal is input to the PC 25 via a DA converter (not shown) or an audio input terminal (not shown). The output signal input to the PC 25 is subjected to fast Fourier transform (FFT) processing and detected as a thermal lens signal.

  Hereinafter, the relationship between the light amount change of the excitation light and the detection light and the intensity change of the thermal lens signal in the thermal lens spectroscopic analysis system 10 will be described.

  In the thermal lens spectroscopic analysis method, since the amount of light absorbed by the detection target substance is measured through the thermal lens phenomenon, the quantity (concentration) of the substance cannot be directly calculated from the thermal lens signal alone. Therefore, by detecting the thermal lens signals of various sample solutions containing the detection target substance at various known concentrations, and using the calibration curve drawn based on it, the detection target contained in the sample solution of unknown concentration The concentration of the substance will be calculated. In this case, since the concentration is determined by the ratio with the measurement result when the calibration curve is drawn, it is important to measure the thermal lens signal of the sample solution of unknown concentration under the same conditions as when the calibration curve is drawn.

  However, the amount of light output from the excitation light source and the detection light source changes due to the influence of changes in the external environment such as a temperature change, or the loss of the optical multiplexer 108 (in each of the excitation light and the detection light, Variation in the thermal lens signal intensity occurs due to a change in the ratio of the input light amount to the output light amount, or the relative positions of the lens 102, the plate-like member 120, and the photoelectric converter 401 being shifted. When such a fluctuation occurs and a sample solution with an unknown concentration is measured under conditions different from those when the calibration curve is measured, the thermal lens signal intensity includes changes due to external fluctuation factors. Can't get. Therefore, it is necessary to correct the thermal lens signal intensity changed by the influence of the external environment change to a value measured under the condition where the calibration curve is measured.

  FIG. 3 is a diagram showing the relationship between the change in the light amount of the excitation light and the detection light and the change in the thermal lens signal intensity in the thermal lens spectroscopic analysis system 10 of FIG. 1, and (a) shows the case where only the light amount of the excitation light changes. It is a figure which shows the relationship between excitation light measurement intensity | strength and thermal lens signal intensity | strength in FIG. 2, (b) is a figure which shows the relationship between detection light measurement intensity | strength and thermal lens signal intensity | strength when only the light quantity of detection light changes. (C) is a figure which shows the relationship between the integrated value of the measurement intensity | strength of excitation light and detection light, and the thermal lens signal intensity | strength when the light quantity of excitation light and the light quantity of detection light change simultaneously.

  In FIG. 3A, the vertical axis indicates the thermal lens signal intensity (mV), the horizontal axis indicates the output (first IV output) (V) of the IV amplifier 23 indicating the measured light quantity of the excitation light, and the thermal lens. The signal strength (mV) is proportional to the first IV output (V). In FIG. 3B, the vertical axis indicates the thermal lens signal intensity (mV), and the horizontal axis indicates the output (second IV output) (V) of the IV amplifier 23 indicating the measurement light quantity of the detection light. The thermal lens signal intensity (mV) is proportional to the second IV output (V). That is, when any one of the excitation light and the detection light changes, the thermal lens signal intensity (mV) is proportional to the first or second IV output (V).

  Here, a method of correcting the thermal lens signal intensity when the amount of excitation light or detection light changes will be described. For example, the predetermined light intensity measurement value of excitation light used when drawing a calibration curve is 2.4 V, the light intensity measurement value of excitation light when measuring a sample solution of unknown concentration is 2.2 V, and the thermal lens signal intensity is Suppose that it was 3.1 mV. In this case, if the calibration curve is compared with the thermal lens signal intensity as it is, the obtained density becomes lower by the amount of decrease in the amount of excitation light. Therefore, the thermal lens signal intensity of the unknown sample solution is corrected to 3.4 mV in view of the change in the amount of excitation light. Here, 3.4 mV is obtained by multiplying the measured value of the thermal lens signal intensity in the sample solution of unknown concentration by the first ratio (predetermined measured light amount of excitation light / measured light amount of measured excitation light). Value, that is, a value calculated from 3.1 × (2.4 / 2.2).

  The thermal lens signal intensity is corrected by a similar method for the change in the amount of detection light.

  In FIG. 3C, the vertical axis represents the thermal lens signal intensity (mV), and the horizontal axis represents the integrated value of the first IV output (V) and the second IV output (V). Is proportional to the thermal lens signal intensity (mV). That is, when the light amounts of the excitation light and the detection light change, the thermal lens signal intensity (mV) is proportional to the integrated value of the first IV output (V) and the second IV output (V).

  In general, it is known that the thermal lens signal intensity is proportional to the excitation light intensity or detection light intensity incident on the sample. However, when the light amounts of the excitation light and the detection light change at the same time, it has not been known what relationship there is between the respective light amounts and the thermal lens signal intensity. As a result of repeated studies, it was found that the thermal lens signal intensity is proportional to the value obtained by multiplying the measured light intensity of the excitation light and the measured light intensity of the detection light, as shown in FIG.

  Here, a method for correcting the thermal lens signal intensity when the light amounts of the excitation light and the detection light are simultaneously changed will be described. For example, when the calibration curve is drawn, the predetermined light intensity measurement value of the excitation light is 2.5 V, the predetermined light intensity measurement value of the detection light is 5.4 V, and the light intensity measurement of the excitation light when measuring a sample solution of unknown concentration It is assumed that the value is 2.2 V, the measured light intensity of the detected light is 4.8 V, and the thermal lens signal intensity is 2.75 mV. In this case, if the calibration curve is compared with the heat lens signal intensity as it is, the obtained density becomes a low value corresponding to the decrease in the light amount of the excitation light and the detection light. Therefore, the thermal lens signal intensity of the sample solution having an unknown concentration is corrected to 3.5 mV in consideration of the change in the light amount of the excitation light and the detection light. Here, 3.5 mV is a first ratio (a predetermined light amount measurement value of excitation light / a measurement light amount measurement value of excitation light) and a second value with respect to the measurement value of the thermal lens signal intensity in the sample solution of unknown concentration. (Multiplied by the integrated value of the ratio of the measured light quantity of the detected light / measured light quantity of the detected light), that is, 2.75 × (2.5 / 2.2) × (5.4 / 4. It is a value calculated from 8).

  In the above explanation, the case where the light quantity (measurement light quantity) of excitation light and detection light when measuring a sample of unknown concentration is lower than the light quantity (predetermined light quantity) when measuring the calibration curve is explained. The same calculation is performed in the same manner when the light amount is higher than the predetermined light amount.

  According to the present embodiment, the measured value of the thermal lens signal intensity, the first ratio (predetermined light amount of excitation light / measured light amount of excitation light), and the second ratio (predetermined light amount of detection light / measurement of detection light) Since the measured value of the thermal lens signal intensity is corrected by integrating the (light quantity), the sample can be accurately measured even if the thermal lens signal intensity changes due to a change in the external environment.

  According to the present embodiment, since the light quantity measurement of the excitation light and the detection light and the measurement of the thermal lens signal are performed by one photoelectric converter 22 (detector), detection necessary for the thermal lens spectroscopic analysis system 10 is performed. Only one instrument is required, and the thermal lens spectroscopic analysis system 10 can be simplified and miniaturized. In addition, since the change in the amount of excitation light and detection light is measured after the excitation light and detection light have passed through the sample, there is no need to place a measurement optical system in the middle of the optical fiber, which is the optical transmission path, and thermal lens spectroscopic analysis The system 10 can be simplified and miniaturized, and changes in the light amounts of the excitation light and the detection light can be accurately measured.

  According to the present embodiment, the measurement of the light amount of the excitation light and the detection light is performed by using the bandpass filters 30 and 31 that transmit only one of the lights, so that it is used in the thermal lens spectroscopic analysis system 10. Correction can be performed only by adding a mechanism for exchanging the two filters to the detecting device 8, so that the thermal lens spectroscopic analysis system 10 can be simplified and miniaturized.

  In this embodiment, bandpass filters 30 and 31 are used as filters that transmit only one of the excitation light and the detection light, but conversely, only one of the excitation light and the detection light is blocked. The filter may be a notch filter, an edge filter, or the like.

  In the present embodiment, the gradient index rod lens 3 is disposed on the microchemical chip 2 above the groove 1 with a predetermined interval. However, the present invention is not limited to this, and the microchemical chip 2 is not limited thereto. It may be placed on the top or may be adhered on the microchemical chip 2.

  In the present embodiment, the output voltage value of the IV amplifier 23 is read by the voltmeter 24. However, the present invention is not limited to this, and the voltage value is measured by measuring the input value to the audio input terminal. Also good. This eliminates the need for installing the voltmeter 24 between the IV amplifier 23 and the PC.

  In the present embodiment, the transmissive member 20 is provided, but the present invention is not limited to this, and the transmissive member 20 may not be provided.

1 is a diagram schematically showing a configuration of a thermal lens spectroscopic analysis system according to an embodiment of the present invention. FIG. It is a figure which shows schematically the structure of the detection apparatus in FIG. It is a figure which shows the relationship between the light quantity change of excitation light and detection light, and the thermal lens signal intensity change in the thermal lens spectroscopic analysis system of FIG. 1, (a) is excitation light measurement intensity | strength when only the light quantity of excitation light changes. (B) is a diagram showing the relationship between the detected light measurement intensity and the thermal lens signal intensity when only the amount of the detection light is changed, and (c) is a diagram showing the relationship between the thermal lens signal strength and the thermal lens signal strength. It is a figure which shows the relationship between the integrated value of the measurement intensity | strength of excitation light and detection light, and thermal lens signal intensity | strength in case the light quantity of excitation light and the light quantity of detection light change simultaneously. It is a figure which shows schematically the structure of the conventional thermal lens spectroscopy analysis system.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Groove 2 Micro chemical chip 3 Refractive index distribution type | mold rod lens 5 Optical fiber 7 Light source unit 10 Thermal lens spectroscopic analysis system 22 Photoelectric converter 25 Personal computer

Claims (12)

  A chip having a groove into which a liquid sample is injected, and an objective lens that collects excitation light and detection light propagated from a light source through an optical transmission path to the liquid sample to generate a thermal lens signal. In a thermal lens spectroscopic analysis system, a light amount measurement unit that measures the light amount of the excitation light and the detection light, a thermal lens signal intensity measurement unit that measures the intensity of the thermal lens signal, a predetermined light amount of the excitation light, and the measurement A ratio calculation unit for calculating a first ratio with the amount of the excited excitation light and a second ratio between the predetermined amount of the detection light and the measured amount of the detection light; and the measured thermal lens signal And a thermal lens signal intensity detection correction unit that corrects the intensity of the measured thermal lens signal by integrating the intensity of the first lens, the first ratio, and / or the second ratio. Lens spectroscopic analysis system   The thermal lens spectroscopic analysis system according to claim 1, wherein the light amount measuring unit that measures the light amounts of the excitation light and the detection light includes a single detector.   2. The light quantity measuring unit for measuring the light quantity of the excitation light and the detection light and the thermal lens signal intensity measuring part for measuring the intensity of the thermal lens signal are composed of one detector. Thermal lens spectroscopic analysis system.   An excitation light transmission filter that transmits only the excitation light and a detection light transmission filter that transmits only the detection light are provided so as to be interchangeable, and the detector includes excitation light transmitted through the light transmission filter and The thermal lens spectroscopic analysis system according to claim 2 or 3, wherein the amount of detection light is measured.   The thermal lens spectroscopic analysis system according to any one of claims 1 to 4, wherein the intensity of the thermal lens signal is corrected based on an output value of the detector.   6. The thermal lens spectroscopic analysis system according to claim 5, wherein the output value of the detector is a current value.   6. The thermal lens spectroscopic analysis system according to claim 5, wherein the output value of the detector is a voltage value.   The thermal lens spectroscopic analysis system according to claim 7, further comprising an audio input terminal for measuring the voltage value.   The thermal lens spectroscopic analysis system according to claim 1, wherein the optical transmission path is an optical fiber.   The thermal lens spectroscopic analysis system according to any one of claims 1 to 9, wherein the objective lens is a rod lens.   The thermal lens spectral analysis system according to claim 1, wherein the thermal lens signal is obtained by a fast Fourier transform process.   In a thermal lens signal correction method for correcting a thermal lens signal generated by irradiating a liquid sample injected into a groove in a chip with excitation light and detection light, a light amount measurement for measuring light amounts of the excitation light and the detection light A thermal lens signal intensity measurement step for measuring the intensity of the thermal lens signal, a first ratio between the predetermined light amount of the excitation light and the measured light amount of the excitation light, and the predetermined light amount of the detection light A ratio calculating step for calculating a second ratio with the measured amount of the detected light, and integrating the measured thermal lens signal intensity, the first ratio, and / or the second ratio. And a thermal lens signal intensity detection correction step for correcting the intensity of the measured thermal lens signal.

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