JP2006111545A - Glutathione reductase activity-enhancing agent - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To obtain a glutathione reductase activity-enhancing agent which enhances glutathione reductase activity in cells to increase the reduced glutathione concentration in a living organism thereby to prevent dermatopathy caused by a peroxide resulting from deficiency of reduced glutathione. <P>SOLUTION: This glutathione reductase activity-enhancing agent comprises extracts of one or more selected from among Leonurus sibiricus L., Glycyrrhiza uralensis, Acanthopanax senticosus, Asiasarum sieboldi F., Oryza sativa L., Cnidium officinale M., lily and Tremella fuciformis B. The glutathione reductase activity-enhancing agent is incorporated into foods, cosmetics, quasi-drugs, pharmaceuticals, or the like, and enhances glutathione reductase activity to accelerate the formation of reduced glutathione thereby to exhibit effectiveness against oxidation injury of the skin or skin ageing. Specifically, the glutathione reductase activity-enhancing agent is effective for improvement of melanism deposition on skin cells and ageing symptoms such as wrinkles or lowering of tightness caused by deterioration in cell functions. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to a glutathione reductase activity enhancer, and in particular, is added to foods, cosmetics, quasi drugs or pharmaceuticals, etc. to enhance the activity of glutathione reductase and promote the production of reduced glutathione. The present invention relates to an antioxidant or preventive agent for living body that exhibits its effectiveness in skin aging.

  The skin is located in the outermost layer of the living body, is an organ in which active oxygen is easily generated due to the influence of ultraviolet rays and the like, and is constantly exposed to the oxygen stress. On the other hand, there are reducing substances typified by glutathione in the skin cells, and the skin cells are protected from the injury of the peroxide unless a peroxide exceeding its ability is generated. Glutathione exists in two types of structures, oxidized and reduced, in the cell. The oxidized glutathione is converted into a reduced form by glutathione reductase, and the intracellular reducing ability is retained. Therefore, in order to maintain the reducing ability of cells, it is important to increase the concentration of reduced glutathione. Glutathione reductase that promotes the production of reduced glutathione is an important enzyme that maintains the reducing ability of cells. It is.

However, it is known that glutathione reductase activity in the skin is reduced by oxidative stress such as ultraviolet rays (Non-patent Document 1). When the glutathione reductase activity decreases, the amount of reduced glutathione produced decreases, and the reducing ability of the cells decreases. Therefore, it is considered that when the injury caused by peroxide surpasses the protective reaction, the skin is oxidized and the cellular function deteriorates and ages.
Jurgen Fuchs M.M. D. , J .; Invest. Dermatol, 93: 769-773, 1989.

Therefore, for the purpose of protecting against injury caused by peroxides, antioxidants and active oxygen scavengers have been studied as in Patent Documents 1 to 4, etc., and active oxygen scavenging enzymes such as SOD and catalase, SOD-like active substances, etc. Food, cosmetics, quasi-drugs, pharmaceuticals, and the like have been developed that contain active oxygen scavengers, antioxidants, and compositions that enhance intracellular glutathione concentrations.
JP-A-9-118630 JP-A-9-208484 JP 2002-275079 A JP 2003-321463 A

  However, active oxygen scavenging enzymes such as SOD and catalase have problems in stability, and active oxygen scavengers and antioxidants are not sufficiently effective. In addition, a composition that enhances the intracellular glutathione concentration is insufficient because a sufficient protective effect against peroxide damage is not expected unless the amount of reduced glutathione is maintained.

  The present invention relates to a glutathione reductase activity enhancer that enhances the activity of intracellular glutathione reductase to increase the biological concentration of reduced glutathione, and prevents peroxide skin damage caused by deficiency of reduced glutathione. The purpose is to provide. Another object of the present invention is to improve aging symptoms such as melanin deposition on skin cells caused by a decrease in cell function and reduction in wrinkles and tension.

  As a result of intensive studies to obtain an external preparation excellent in the protective action against peroxide-induced injury, the present inventors have selected one kind selected from among the reeds, licorice, sorghum, saicin, rice, senkyu, lily and white jellyfish Alternatively, the inventors have found that two or more extracts are stable and enhance the glutathione reductase activity in skin cells to increase the biological concentration of reduced glutathione, thereby completing the present invention.

  Glutathione reductase activity enhancer characterized in that it contains one or more extracts selected from the scorpionfish, licorice, sorghum, saicin, rice, nematode, lily and white jellyfish of the present invention, the activity of glutathione reductase, Has the effect of increasing the biological concentration of reduced glutathione. More specifically, the present invention relates to a glutathione reductase activity potentiator that is effective in improving aging symptoms such as melanin deposition on skin cells caused by a decrease in cell function and reduction in wrinkles and tension.

  The pheasant used in the present invention (scientific name: Leonurus sibiricus L.) is a herb belonging to the genus Lamiaceae, and grows naturally in sunny places in Yamano from Honshu, Shikoku, Kyushu, Korea and China to Southeast Asia. The whole grass of Mejijiki can be used because it is distributed under the name of Yakumosou, and the mature fruit is distributed under the name of Jujushi.

  Licorice (scientific name: Glycyrrhiza uralensis) used in the present invention is a herb belonging to the genus Licorice, and is distributed from Africa, Europe to Asia, Australia, and western North America. Roots and running stalks (strons) can be used in Chinese medicine called licorice. You may use closely related licorice (scientific name: Glycyrrhiza glabra).

  Ezoukogi (scientific name: Acanthopanax senticosus) used in the present invention is a shrub belonging to the genus Uguigiaceae, and is distributed from East Asia to Southeast Asia and the Philippines. The root bark distributed as a crude drug can be used.

  The saicin used in the present invention is a kalin saicin (scientific name: Asiasarum heterotropoids F.) or usbasaicin (scientific name: Asiasarum sieboldi F.) of the genus Euphoridae, which is obtained by drying rhizomes and roots, or whole rooted plants (fine cells). Can be used.

  The rice used in the present invention is the fruit of Oryza sativa L belonging to the family Gramineae, and brown rice or rice bran can be used.

  The senkyu (scientific name: Cnidium officinale M. or Ligusticum chuaxiong H.) used in the present invention is a herb belonging to the ciraceae family and is distributed in China and Japan. The dried rhizome used as a crude drug (Ryukyu) can be used.

  The lily used in the present invention belongs to the genus Lily of the family Lilyaceae. For example, Madonna Lily (scientific name: Lilium candidum) or Hakatayuri (scientific name: Lilium brownii FE), Himeyuri (scientific name: Lilium controller S.) Lilium lancifolium T.), dried lily bulb (scientific name: Lilium auratum L.) bulbs (herbal medicine name: lily) and the like can be used.

  The white jellyfish used in the present invention (scientific name: Tremella fuciformis B.) is a mushroom belonging to the family Asteraceae, and can use fruit bodies, fungus bodies, and the like.

  In addition, the extract of swordfish, licorice, sorghum, saicin, rice, senkyu, lily and white jellyfish used in the present invention can be obtained by, for example, immersing or heating a plant with an extraction solvent, filtering, and concentrating if necessary. It is done. Examples of the extraction solvent include water, lower monohydric alcohols (methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, etc.), liquid polyhydric alcohols (1,3-butylene glycol, propylene). Glycol, etc.), hydrocarbons (hexane, pentane, etc.), ketones (acetone, methyl ethyl ketone, etc.), ethers (ethyl ether, tetrahydrofuran, propyl ether, etc.), acetonitrile and the like. These solvents may be used alone or in combination of two or more. Preferably, one or two or more of water or a water-soluble solvent (solvent that can be mixed with water in an arbitrary ratio. For example, ethanol, 1,3-butylene glycol, propylene glycol, etc.) may be used. . The extract may be used as it is, or a part or all of the solvent may be distilled off.

  As the glutathione reductase activity enhancer of the present invention, the above-mentioned extracts of rainbow trout, licorice, sorghum, saicin, rice, nematode, lily and white jellyfish may be used as they are, and within the range not impairing the effects, ordinary external preparations Fats and oils, waxes, hydrocarbons, fatty acids, alcohols, esters, surfactants, metal soaps, pH adjusters, preservatives, perfumes, moisturizers, powders, UV absorbers Ingredients such as thickeners, pigments, antioxidants, whitening agents and chelating agents can also be blended.

  The glutathione reductase activity enhancer used in the present invention can be used in any of foods, cosmetics, quasi drugs, and pharmaceuticals. Examples of the dosage form include tablet confectionery, beverages, lotions, creams, emulsions, Gel, aerosol, ointment, poultice, paste, plaster, essence, pack, cleaning agent, bath preparation, foundation, powder, lipstick, powder, pill, tablet, injection, suppository, emulsion, capsule , Granules, liquids (including tinctures, fluid extracts, spirits, suspensions, limonades, etc.).

  The compounding amount of the extract of pheasant, licorice, sorghum, saicin, rice, senkyu, lily and white jellyfish used in the present invention is not particularly limited, but is preferably in the range of 0.0001 to 75% by weight as a dry product, more preferably 0. 0.001 to 30% by weight. If it is 0.0001% by weight or less, the effect is low, and even if it exceeds 75% by weight, the effect is hardly increased and it is not efficient. The addition method may be added in advance or during the production, and may be appropriately selected in consideration of workability.

  Next, in order to describe the present invention in detail, examples of production of the extract used in the present invention, formulation examples and experimental examples will be given as examples, but the present invention is not limited thereto. The compounding amount shown in the examples indicates% by weight.

Production Example 1 Mehojiki Ethanol Extract 900 ml of 30% ethanol was added to 100 g of beneficial herb, and extracted at room temperature for 7 days, followed by filtration. The filtrate was concentrated to dryness to obtain 7.5 g of Mejijiki ethanol extract. .

Production Example 2 Licorice ethanol extract 900 mL of 30% ethanol was added to 100 g of licorice, extracted at room temperature for 7 days, filtered, and the filtrate was concentrated to dryness to obtain 6.0 g of licorice ethanol extract. .

Production Example 3 Ezoukogi Ethanol Extract 900 g of 30% ethanol was added to 100 g of dried dried elephant root bark, extracted at room temperature for 7 days, filtered, and the filtrate was concentrated to dryness to obtain an ethanol extract of Ekokogi. Of 6.0 g was obtained.

Production Example 4 Ethanol extract of saicin 900 mL of 30% ethanol was added to 100 g of fine spices, extracted at room temperature for 7 days, filtered, and the filtrate was concentrated to dryness to obtain 4.9 g of saicin ethanol extract. It was.

Production Example 5 Rice ethanol extract 900 mL of 30% ethanol was added to 100 g of dried rice bran, extracted at room temperature for 7 days, filtered, the filtrate was concentrated to dryness, and the rice ethanol extract 7 .5 g was obtained.

Manufacture example 6 Ethanol extract of nematode 900 mL of 30% ethanol was added to 100 g of river cucumber, extracted at room temperature for 7 days, filtered, and the filtrate was concentrated to dryness to obtain 6.3 g of ethanol extract of nematode. It was.

Production Example 7 Lily ethanol extract To 100 g of dried Madonna lily bulbs, 900 mL of 30% ethanol was added, extracted at room temperature for 7 days, filtered, the filtrate was concentrated to dryness, and lily ethanol extract 5.4g was obtained.

Production Example 8 Ethanol extract of white jellyfish 900 mL of 30% ethanol was added to 100 g of dry matter of white jellyfish, extracted at room temperature for 7 days, filtered, and the filtrate was concentrated to dryness to obtain ethanol extract of white jellyfish. 3.4 g was obtained.

Production Example 9 Hot water extract of Japanese reptile 400 ml of purified water was added to 20 g of beneficial mother grass, extracted at 95-100 ° C. for 2 hours, filtered, and the filtrate was concentrated and lyophilized to obtain a hot water extract of Japanese pheasant. 2.2 g was obtained.

Production Example 10 Licorice hot water extract 400 mL of purified water was added to 20 g of licorice, extracted at 95-100 ° C. for 2 hours, filtered, and the filtrate was concentrated and lyophilized to obtain a licorice hot water extract. 1.0 g was obtained.

Production Example 11 Hot water extract of Ekokogi 400 mL of purified water was added to 20 g of dried dried Ezokogi bark, extracted at 95-100 ° C. for 2 hours, filtered, the filtrate was concentrated, lyophilized and dried. 1.8 g of a hot water extract was obtained.

Production Example 12 Hot water extract of saicin 400 mL of purified water was added to 20 g of fine spices, extracted at 95-100 ° C. for 2 hours, filtered, the filtrate was concentrated, freeze-dried, and hot water extract of saicin. 1.5g was obtained.

Production Example 13 Hot water extract of rice 400 mL of purified water was added to 20 g of dried rice bran, extracted at 95-100 ° C. for 2 hours, filtered, the filtrate was concentrated, freeze-dried, 3.3 g of water extract was obtained.

Production Example 14 Hot water extract of Senkyu 400 ml of purified water was added to 20 g of Kawakyu, extracted at 95-100 ° C. for 2 hours, filtered, the filtrate was concentrated, freeze-dried, and hot water extract of Senkyu. Of 1.8 g was obtained.

Production Example 15 Lily Hot Water Extract 400 mL of purified water was added to 20 g of dried Madonna lily bulbs, extracted at 95-100 ° C. for 2 hours, filtered, the filtrate was concentrated, freeze-dried and lily 0.3 g of a hot water extract was obtained.

Manufacture example 16 Hot water extract of white jellyfish 4 L of purified water was added to 20 g of dried fruit body of white jellyfish, extracted at 95-100 ° C. for 2 hours, filtered, and the filtrate was concentrated, lyophilized and dried. 1.5 g of a hot water extract was obtained.

Production Example 17 1,3-Butyleneglycol aqueous solution extract of pheasants 1 kg of purified water and 1 kg of 1,3-butyleneglycol were added to 100 g of dried whole pheasant grass, extracted at room temperature for 7 days, filtered, 1.9 kg of a 1,3-butylene glycol aqueous extract of pheasantfish was obtained.

Production Example 18 Licorice 1,3-butylene glycol aqueous solution extract 1 kg of purified water and 1 kg of 1,3-butylene glycol were added to 100 g of dried licorice leaves, extracted at room temperature for 7 days, filtered, and licorice 1.9 kg of a 1,3-butylene glycol aqueous solution extract was obtained.

Production Example 19 1,3-Butyleneglycol aqueous solution extract of Ekokogi 1 kg of purified water and 1 kg of 1,3-butyleneglycol were added to 100 g of dried dried whole of Ekogigi, extracted at room temperature for 7 days, filtered, 1.9 kg of a 1,3-butylene glycol aqueous extract of Ezoukogi was obtained.

Production Example 20 1,3-Butylene Glycol Aqueous Extract of Saicin 1 kg of purified water and 1 kg of 1,3-butylene glycol were added to 100 g of fine spicy, extracted at room temperature for 7 days, filtered, and filtered. -1.9 kg of butylene glycol aqueous solution extract was obtained.

Production Example 21 Rice 1,3-butylene glycol aqueous solution extract 1 kg of purified water and 1 kg of 1,3-butylene glycol were added to 100 g of dried rice bran, extracted for 7 days at room temperature, filtered, 1.9 kg of 1,3-butylene glycol aqueous solution extract was obtained.

Production Example 22 Extract of 1,3-butylene glycol aqueous solution of nematode 1 kg of purified water and 1 kg of 1,3-butylene glycol were added to 100 g of river cucumber, extracted at room temperature for 7 days, filtered, and 1,3 butylene glycol 1,3 -1.9 kg of butylene glycol aqueous solution extract was obtained.

Production Example 23 Lily 1,3-butylene glycol aqueous extract 1 kg purified water and 1 kg 1,3-butylene glycol were added to 100 g of dried dried lily whole plant, extracted at room temperature for 7 days, filtered, 1.9 kg of lily 1,3-butylene glycol aqueous solution extract was obtained.

Production Example 24 1,3-butylene glycol aqueous extract of white jellyfish 1 kg of purified water and 1 kg of 1,3-butylene glycol were added to 100 g of dry matter of white jellyfish fruit bodies, extracted at room temperature for 7 days, filtered, 1.9 kg of a 1,3-butylene glycol aqueous extract of white jellyfish was obtained.

  Next, the prescription of the Example which concerns on this invention is shown.

Formulation Example 1 Cream Formulation Amount (% by weight)
1. Shrimp ethanol extract (Production Example 1) 0.5
2. Squalane 5.5
3. Olive oil 3.0
4). Stearic acid 2.0
5. Beeswax 2.0
6). Octyldodecyl myristate 3.5
7). Polyoxyethylene cetyl ether (20E.O.) 3.0
8). Behenyl alcohol 1.5
9. Glycerol monostearate2.5
10. Fragrance 0.1
11.1,3-butylene glycol 8.5
12 Ethyl paraoxybenzoate 0.05
13. Methyl paraoxybenzoate 0.2
14 Purified water 67.65
[Manufacturing method] Components 1 to 9 are heated and mixed to maintain an oil phase at 70 ° C. Ingredients 11-14 are dissolved by heating and mixed, and kept at 75 ° C. to form an aqueous phase. The aqueous phase is added to the oil phase to emulsify, and the mixture is cooled while stirring. The component 10 is added at 45 ° C, and further cooled to 30 ° C to obtain a product.

Formulation Example 2 Cream 2
In Formulation Example 1, cream 2 was obtained by replacing the ethanol extract of Japanese cypress with the ethanol extract of licorice (Production Example 2).

Formulation Example 3 Cream 3
In Formulation Example 1, cream 3 was obtained by substituting ethanol extract of swordfish with ethanol extract of Ezokogi (Production Example 3).

Formulation Example 4 Cream 4
In Formulation Example 1, cream 4 was obtained by replacing the ethanol extract of swordfish with the ethanol extract of Saicin (Production Example 4).

Formulation Example 5 Cream 5
In Formulation Example 1, cream 5 was obtained by replacing the ethanol extract of Japanese pheasant with an ethanol extract of rice (Production Example 5).

Formulation Example 6 Cream 6
In Formulation Example 1, cream 6 was obtained by replacing the Japanese extract of Japanese marlin with an ethanol extract of Senkyu (Production Example 6).

Formulation Example 7 Cream 7
In Formulation Example 1, cream 7 was obtained by replacing the ethanol extract of Japanese cypress with the ethanol extract of lily (Production Example 7).

Formulation Example 8 Cream 8
In Formulation Example 1, cream 8 was obtained by replacing the ethanol extract of white jellyfish with the ethanol extract of white jellyfish (Production Example 8).

Comparative Example 1 Conventional Cream In Formulation Example 1, a conventional cream was prepared by substituting the ethanol extract of a swordfish with purified water.

Formulation Example 9 Lotion Formulation Amount (% by weight)
1. 1,3-butylene glycol aqueous solution extract of mehajiki (Production Example 17) 0.1
2. 1,3-butylene glycol 8.0
3. Glycerin 2.0
4). Xanthan gum 0.02
5. Citric acid 0.01
6). Sodium citrate 0.1
7). Ethanol 5.0
8). Methyl paraoxybenzoate 0.1
9. Polyoxyethylene hydrogenated castor oil (40E.O.) 0.1
10. Fragrance 0.1
11. Purified water 84.47
[Production Method] Components 2 to 6 and 11 and Components 1 and 7 to 10 are uniformly dissolved, and both are mixed and filtered to obtain a product.

Formulation Example 10 Emulsion Formulation Amount (% by weight)
1. Licorice hot water extract (Production Example 10) 0.05
2. Squalane 5.0
3. Olive oil 5.0
4). Jojoba oil 5.0
5. Cetanol 1.5
6). Glycerol monostearate 2.0
7). Polyoxyethylene cetyl ether (20E.O.) 3.0
8). Polyoxyethylene sorbitan monooleate 2.0
9. Fragrance 0.1
10. Propylene glycol 1.0
11. Glycerin 2.0
12 Methyl paraoxybenzoate 0.2
13. Purified water 73.15
[Manufacturing method] Components 2 to 8 are dissolved by heating and mixed, and kept at 70 ° C to obtain an oil phase. Ingredients 1 and 10-13 are dissolved by heating and mixed, and kept at 75 ° C. to form an aqueous phase. The aqueous phase is added to the oil phase to emulsify, and the mixture is cooled while stirring.

Formulation Example 11 Ointment Formulation Amount (% by weight)
1. 1,3-butylene glycol aqueous solution extract of Ekokogi (Production Example 19) 1.0
2. Polyoxyethylene cetyl ether (30E.O.) 2.0
3. Glycerol monostearate 10.0
4). Liquid paraffin 5.0
5. Cetanol 6.0
6). Methyl paraoxybenzoate 0.1
7). Propylene glycol 10.0
8). Purified water 65.9
[Manufacturing method] Components 2 to 5 are dissolved by heating and mixed, and kept at 70 ° C to obtain an oil phase. Ingredients 1 and 6 to 8 are dissolved by heating and mixed, and kept at 75 ° C. to obtain an aqueous phase. The aqueous phase is added to the oil phase to emulsify, and the mixture is cooled to 30 ° C. with stirring to obtain a product.

Formulation Example 12 Foundation Formulation Amount (% by weight)
1. 1,3-Butylene glycol aqueous solution extract of saicin (Production Example 20) 1.0
2. Stearic acid 2.4
3. Polyoxyethylene sorbitan monostearate 1.0
(20 EO)
4). Polyoxyethylene cetyl ether (20E.O.) 2.0
5. Cetanol 1.0
6). Liquid lanolin 2.0
7). Liquid paraffin 3.0
8). Isopropyl myristate 6.5
9. Butyl paraoxybenzoate 0.1
10. Sodium carboxymethylcellulose 0.1
11. Bentonite 0.5
12 Propylene glycol 4.0
13. Triethanolamine 1.1
14 Methyl paraoxybenzoate 0.2
15. Titanium dioxide 8.0
16. Talc 4.0
17. Bengala 1.0
18. Yellow iron oxide 2.0
19. Fragrance 0.1
20. Purified water 60.0
[Manufacturing method] Components 2 to 9 are heated and dissolved and kept at 80 ° C to obtain an oil phase. Swell component 10 well with component 20, then add components 1 and 11-14 and mix uniformly. To this, components 15 to 18 pulverized and mixed with a pulverizer are added, and the mixture is stirred with a homomixer and kept at 75 ° C. to obtain an aqueous phase. The oily phase is added to the aqueous phase with stirring, cooled, and component 19 is added at 45 ° C, and cooled to 30 ° C with stirring to give a product.

Formulation Example 13 Bath preparation formulation Formulation amount (% by weight)
1. Rice hot water extract (Production Example 13) 5.0
2. Sodium bicarbonate 50.0
3. Yellow No. 202 (1) 0.05
4). Fragrance 0.25
5. Anhydrous sodium sulfate 44.7
[Production method] Components 1 to 5 are uniformly mixed to obtain a product.

Formulation Example 14 Tablet Confectionery Formulation Amount (% by weight)
1. Senkyu hot water extract (Production Example 14) 2.0
2. Dried corn starch 50.0
3. Erythritol 40.0
4). Citric acid 5.0
5. Sucrose fatty acid ester 3.0
6). Perfume appropriate amount 7. Water Appropriate amount [Production method] Components 1 to 4 and 7 are mixed and granulated. Ingredients 5 and 6 are added to the formed granules and compressed. One tablet is 1.0 g.

Formulation Example 15 Beverage Formulation Amount (% by weight)
1. Lily hot water extract (Production Example 15) 1.0
2. Stevia 0.05
3. Malic acid 5.0
4). Fragrance 0.1
5. [Production method] Components 2 and 3 are dissolved in a small amount of water. Components 1 and 4, 5 are then added and mixed.

Formulation Example 16 Tablet formulation Formulation amount (% by weight)
1. White water jellyfish extract (Production Example 16) 10.0
2. Corn starch 10.0
3. Purified white sugar 20.0
4). Carboxymethylcellulose 10.0
5. Microcrystalline cellulose 35.0
6). Polyvinylpyrrolidone 5.0
7). Talc 10.0
[Production Method] Components 1 to 5 were mixed, and then an aqueous solution of Component 6 was added as a binder and granulated by a conventional method. This was mixed with ingredient 7 as a lubricant and then tableted into 100 mg tablets.

Formulation Example 17 Powder Formulation Amount (% by weight)
1. Sea water extract of hot water (Production Example 9) 10.0
2. Corn starch 40.0
3. Microcrystalline cellulose 50.0
[Production method] The above components were mixed and powdered by a conventional method.

Formulation Example 18 Injection formulation Formulation amount (% by weight)
1. Licorice hot water extract (Production Example 10) 1.0
2. Polyoxyethylene (60) hydrogenated castor oil 3.7
3. Sesame oil 0.2
4). Sodium chloride 0.9
5. Propylene glycol 4.0
6). Phosphate buffer (0.1 M, pH 6.0) 10.0
7). Distilled water 80.2
[Production method] Components 1, 2, 3 and half of component 5 are mixed and heated to dissolve at about 80 ° C, and components 7, 6 and 5 previously dissolved therein are heated to about 80 ° C. The total amount was 1000 mL of an aqueous solution. This aqueous solution was dispensed into 1 mL ampules, melted and then sterilized by heating.

  Next, experimental examples will be given to explain the effects of the present invention in detail.

Experimental Example 1 Activation Test of Intracellular Glutathione Reductase The activation effect of intracellular glutathione reductase was measured under the following conditions.

  Confluent human epidermal keratinocytes were further cultured in DMEM medium containing 1 μg / mL sample for 24 hours, then the cells were detached, and 1 mL of 50 mM phosphate buffer (pH 7.5) containing 1 mM EDTA was added. The mixture was sonicated and centrifuged at 15,000 rpm for 15 minutes, and the supernatant was used as a sample solution. To 900 μL of the sample solution, 100 μL of 9.5 mM oxidized glutathione / phosphate buffer solution and 120 μL of 2 mM NADPH / phosphate buffer solution were added, and the time-dependent change in absorbance at 340 nm (φA 340 nm / min, 3 minutes) was measured, and standard glutathione reductase was measured. The glutathione reductase activity of the sample solution was calculated from a calibration curve prepared from 0, 5, 10 and 20 mU / mL. At the same time, the protein concentration of the sample solution was measured, and the glutathione reductase activity per unit protein was calculated. The glutathione reductase activity of the cells not added with the sample was used as a control, and the value of the glutathione reductase activity with the sample added when the control was 100 was calculated as the intracellular glutathione reductase activity.

  The test results are shown in Table 1. As a result, an excellent activation action of intracellular glutathione reductase was observed in the extracts of swordfish, licorice, sorghum, saicin, rice, senkyu, lily and white jellyfish. Moreover, when the extract of manufacture example 9-24 was tested, all showed the activation effect | action of the outstanding intracellular glutathione reductase.

Experimental Example 2 Evaluation of increasing action of intracellular reduced glutathione ratio The increasing action of intracellular reduced glutathione ratio was measured under the following conditions.

  The epidermal keratinocytes in a confluent state were further cultured for 24 hours in Eagle's MEM medium supplemented with a 1 μg / mL concentration sample, and then the cells were detached and 50 mM phosphate buffer (pH 7) containing 1 mM EDTA. 5) 0.5 mL was added, and the mixture was sonicated and centrifuged at 15,000 rpm for 15 minutes, and the supernatant was used as a sample solution. Using a 96-well plate, 25 μL of a 1: 1 mixture of 0.5 mM NADPH / phosphate buffer solution and 1 U / mL glutathione reductase / phosphate buffer solution was added to 100 μL of the sample solution, and reacted at room temperature for 10 minutes. Further, 25 μL of 10 mM 5,5′-dithiobis-2-nitrobenzoic acid / phosphate buffer solution was added and reacted at room temperature for 10 minutes, and then the absorbance at 405 nm was measured. The total glutathione amount of the sample solution was calculated from a calibration curve prepared from standard reduced glutathione (GSH). Further, 2.5 μL of 1M2-vinylpyridine was added to 150 μL of the sample solution and reacted at room temperature for 1 hour to remove GSH. Then, the amount of oxidized glutathione (GSSG) was determined by the same reaction. At the same time, the protein concentration of the sample solution was measured, the GSH amount was calculated from the total glutathione amount per unit protein and the GSSG amount, and the GSH / GSSG ratio was determined. The GSH / GSSG ratio of the cells not added with the sample was used as a control, and the value of the GSH / GSSG ratio with the sample added when the control was 100 was calculated as the intracellular reduced glutathione ratio.

  The test results are shown in Table 2. As a result, the extract of scorpionfish, licorice, sorghum, saicin, rice, senkyu, lily and white jellyfish has an excellent effect of increasing the ratio of intracellular reduced glutathione, and the effect of increasing the ratio of reduced glutathione in the cells. It was. Moreover, when the extract of the manufacture examples 9-24 was tested, all showed the increase effect of the intracellular reduced glutathione ratio which was excellent.

Experimental Example 3 Use Test Using the creams of Formulation Examples 1 to 8 and the conventional cream of Comparative Example 1, a use test for 6 months was conducted on 30 women (20 to 45 years old) suffering from wrinkles and spots. . After use, a questionnaire survey on wrinkle and blemishes was conducted to determine the effect of improving skin properties. The evaluation criteria of the questionnaire were evaluated as “excellent” for valid, “good” for slightly effective, “good” for slightly effective, and “impossible” for invalid.

  These results are shown in Table 3. The external preparation for skin containing the glutathione reductase activity enhancer of Formulation Examples 1 to 8 showed excellent wrinkle and stain improvement effects. During the test period, there was no skin problem and there was no problem with safety.

  When the usage tests of the lotions, emulsions, ointments, foundations, bath preparations, tablet confectionery, beverages, tablets, and powders of Formulation Examples 9 to 17 were conducted, all showed safe and excellent wrinkle and stain improvement effects.

As an application example of the present invention, it can be used for any of foods, cosmetics, quasi drugs, and pharmaceuticals. The dosage forms include tablet confectionery, beverages, lotions, creams, emulsions, gels, aerosols, ointments, poultices, pastes, plasters, essences, packs, cleaning agents, bath preparations, foundations, powders, lipsticks. , Powders, pills, tablets, injections, suppositories, emulsions, capsules, granules, liquids (including tinctures, fluid extracts, spirits, suspensions, limonades, etc.) Improvement effects such as prevention of skin wrinkles and spots caused by a reduction in the reducing ability of the cells can be obtained.

Claims (5)

An activity enhancer for glutathione reductase, comprising one or more extracts selected from among mejijiki, licorice, sorghum, saicin, rice, senkyu, lily and white jellyfish. 1. A reduced glutathione production promoter characterized by containing one or more extracts selected from mejijiki, licorice, sorghum, saicin, rice, senkyu, lily and white jellyfish. An antioxidant by promoting production of reduced glutathione comprising the glutathione reductase activity enhancer according to claim 1. A whitening composition by promoting the production of reduced glutathione comprising the glutathione reductase activity enhancer according to claim 1. A composition for preventing aging by promoting the production of reduced glutathione comprising the glutathione reductase activity enhancer according to claim 1.