JP2006022068A - Serum lipid metabolism ameliorative agent - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a nutritive composition having serum lipid metabolism ameliorative effect. <P>SOLUTION: The nutritive composition comprises proteins, lipids and carbohydrates. In this composition, energy proportion is represented by 10-25% proteins, 10-35% lipids and 40-60% carbohydrates, and oleic acid in the energy proportion of the lipids stands at 60-90% and palatinose and/or trehalose in the energy proportion of the carbohydrates stand(s) at 60-100%. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to a composition having an effect of improving serum lipid metabolism and a food to which an effective amount of the composition is added.

In recent years, the incidence of ischemic heart diseases such as angina pectoris and myocardial infarction caused by arteriosclerosis, particularly coronary arteriosclerosis, has been increasing. In addition to hyperlipidemia, risk factors such as hypertension, smoking, stress, diabetes, and hereditary predisposition are true factors that cause arteriosclerosis. Is the greatest risk factor. Hyperlipidemia is defined as a state in which one or both of cholesterol and triglyceride (neutral fat) in serum lipid components, or both are increased more than normal.
It is said that the higher the serum cholesterol concentration or LDL cholesterol concentration, the higher the risk of arteriosclerosis, and the lower the serum cholesterol level, the lower the incidence of coronary artery disease. Also, among serum lipoproteins, the now generalized notion that LDL works aatherogenic and HDL works antiatherogenic has been established by epidemiological studies. And although the low HDL cholesterol correlates with the high value of lipid, the evaluation of triglyceride as a risk factor is too low (Non-patent Document 1). In familial hypercholesterolemia, it has been shown that a higher rate of combined ischemic heart disease and cerebrovascular disorder in the high triglyceride group, and is at the level of borderline hypercholesterolemia In some cases, it is desirable to improve the triglyceride concentration. Therefore, in order to prevent or ameliorate hyperlipidemia, it is necessary to lower blood triglycerides. Hyperlipidemia is also known as an exacerbation factor of diabetic nephropathy.
The latest internal medicine university volume 36 arteriosclerosis and vascular disease, p20, Nakayama Shoten (1991)

  An object of the present invention is to provide a lipid metabolism improving composition having a triglyceride lowering action and a food to which an effective amount of the composition is added.

  Therefore, the present inventors prepared a nutritional composition containing proteins, lipids and carbohydrates at a specific energy ratio, and investigated the effect on lipid metabolism in normal animals. As a result, the nutritional composition showed a significant inhibitory effect on serum triglyceride elevation compared with the liquid food “May Balance C (FOOD Style 21, pp.58, 1991.1 (Vol.3 No.1))”. From these results, the present nutritional composition was found to be useful for controlling serum lipids for those with abnormal serum lipid metabolism and for preventing serum lipid elevation, and completed the present invention. The nutritional composition has been shown to have a blood glucose level controlling action (WO 03/022288), but it has not been known to have a serum lipid metabolism improving action.

That is, the present invention
[1] A nutritional composition containing protein, lipid and carbohydrate, wherein the energy ratio is 10 to 25% protein, 10 to 35% lipid and 40 to 60% carbohydrate, and the energy ratio of lipid A nutritional composition for improving serum lipid metabolism, wherein oleic acid is 60-90%, palatinose and / or trehalulose in the carbohydrate energy ratio is 60-100%,
[2] The nutritional composition according to the above [1], comprising at least one selected from milk phospholipid, soybean lecithin, high oleic sunflower oil and egoma oil,
About.

  Since the nutritional composition of the present invention has an excellent effect of improving serum lipid metabolism, it is useful as an oral / tube feeding agent, a therapeutic food, a food for the sick for home use or a health functional food.

  In the nutritional composition of the present invention (hereinafter also referred to as “nutrient composition” or “composition”), protein is contained in the composition at an energy ratio of 10 to 25%, preferably at an energy ratio of 10 to 20%. The

  Examples of the protein include milk protein, plant-derived protein, soy protein or a hydrolyzate thereof, and milk protein is common. As milk proteins, MPC (Milk Protein Concentrate), casein protein, whey protein, magnesium caseinate and their hydrolysates, fermented milk and components obtained by removing whey from fermented milk (fresh cheese, quark) -252896). Of these, MPC, or a combination of MPC and casein is most preferable.

  As whey protein, whey powder concentrated whey, dried whey protein concentrate (WPC) after concentrated whey ultrafiltration (UF), after removing fat in whey, UF-concentrated defatted WPC (low-fat and high-protein), WPI that selectively separates protein from whey, nanofiltration-concentrated desalted whey, mineral-enriched whey that is enriched with mineral components derived from whey, etc. It is done.

  In the nutritional composition of the present invention, the lipid is contained in the composition at an energy ratio of 10 to 35%, preferably at an energy ratio of 20 to 30%. This ratio is in accordance with the nutritional requirements of Japanese people in the 6th revision. In order to increase the content of monounsaturated fatty acids (MUFA) in the fatty acid composition of lipids, oleic acid, which is a monounsaturated fatty acid, has an energy ratio of 60 to 90%, preferably 60 to 80% in the lipid. Contained in proportions. Examples of lipid sources rich in oleic acid include high oleic sunflower oil, rapeseed oil, olive oil, high oleic safflower oil, soybean oil, corn oil and palm oil. Moreover, nutrition preparation fats and oils (made by Nippon Oil & Fats Co., Ltd.) are mentioned as a lipid source containing oleic acid. Sunflower oil, rapeseed oil, olive oil, and mixtures with olive oil can also be used.

  As other lipids, phospholipids derived from milk and lecithin (derived from soybean or egg yolk) are preferably used.

  Milk phospholipids are localized only in the milk fat globule membrane (MFGM). Free of butter oil from lyophilized and whey cream of WPI by-product (MF retentate) produced by a combination of ultrafiltration (UF) and microfiltration (MF). A fraction (bataselam) etc. are mentioned. A lipid fraction extracted several times with ethanol from Bataselam or concentrated, or an acetone-insoluble fraction (α-Lipid: Anchor Pruducts, New Zealand) may be used.

  Lecithin chemically means phosphatidylcholine (PC), but usually, in addition to this PC, four types of phosphatidylethanolamine (PE), phosphatidylinositol (PI), and phosphatidic acid (PA) and other phosphorus A mixture of lipids is referred to as lecithin, and all of these lecithins can be used in the present invention. In addition to this, paste-like acetone-insoluble fraction, which is an index of phospholipid purity, is 62 to 65%, powdered high-purity lecithin with phospholipid content of 95% or more, fractionated lecithin with increased phosphatidylcholine content, etc. Is mentioned.

  The composition of the present invention can contain an n-6 series polyunsaturated fatty acid and an n-3 series polyunsaturated fatty acid. Preferably, these polyunsaturated fatty acids can be contained in the fatty acid composition at 10 to 40%, preferably 10 to 30%. For example, these polyunsaturated fatty acids can comprise about 20% in the fatty acid composition.

  The lipid composition of the nutritional composition is such that the mixing ratio of the n-6 series polyunsaturated fatty acid and the n-3 series polyunsaturated fatty acid is about 5: 1 to about 1: 1, preferably about 4: 1. be able to. For this purpose, it is preferable to blend sesame oil (seed oil), linseed oil or the like having a high content ratio of n-3 α-linolenic acid. Skipjack and tuna oil containing a large amount of DHA may be used.

  In the present invention, it is preferable to use at least one selected from milk phospholipid, soybean lecithin, high oleic sunflower oil, and egoma oil as the lipid.

  In the nutritional composition of the present invention, carbohydrates are contained in the composition at an energy ratio of 40 to 60%, preferably 50 to 60%. This energy ratio is almost in line with the nutritional requirements of Japanese people in the 6th revision. As the saccharide, palatinose, trehalulose or a mixture thereof is used. Palatinose, trehalulose or a mixture thereof is contained in the carbohydrate at an energy ratio of 60 to 100%, preferably 60 to 80%.

  Other carbohydrates include, for example, sugar alcohols (sorbitol, xylitol, maltitol, etc.), trehalose, palatinit, maltodextrin, modified starch, amylose starch, tapioca starch, corn starch, fructose, lactose, or mixtures thereof. Can be mentioned. Of these, maltodextrin, xylitol or a mixture thereof is preferred. Maltodextrins are intermediate sugars obtained by acid hydrolysis or enzymatic degradation of starch or corn starch and have a DE value of 20 or less.

  The nutritional composition of the present invention can further contain dietary fiber. As dietary fiber, either water-soluble dietary fiber or water-insoluble dietary fiber may be used. Product, galactomannan and the like. Indigestible dextrins are preferred because they are easy to add to foods and do not hinder food processing. Examples of the water-insoluble dietary fiber include crystalline cellulose, soybean dietary fiber, wheat bran, corn fiber, and beet fiber.

Moreover, the nutrition composition of this invention can contain the vitamin and mineral according to a standard liquid food compounding quantity. Examples of vitamins include vitamin B ₂ , nicotinamide, vitamin B ₆ , calcium pantothenate, folic acid, vitamin B ₁₂ , vitamin A fatty acid ester, vitamin D ₃ , α-vitamin E, vitamin K ₂ and sodium L-ascorbate. , Β-carotene and the like. Minerals include calcium, phosphorus, iron, sodium, potassium, chlorine, magnesium, or trace elements derived from natural products such as yeast minerals such as copper, zinc, selenium, manganese, and chromium. Copper gluconate, zinc gluconate and the like can also be used.

The osmotic pressure of the nutritional composition of the present invention preferably has an osmotic pressure of about 200 to 1000 mOsm / L, such as about 300 to 750 mOsm / L. When measured at room temperature, the nutritional composition preferably has a viscosity of about 5 to 40 mPa · s, particularly 5 to 20 mPa · s.
Moreover, it is preferable that the calorie of a nutritional composition is about 0.5-3 kcal / mL, especially 1-1.5 kcal / mL.

  The nutritional composition is preferably in a form that can be used directly. In this form, the composition can be taken orally via the nose-stomach, jejunum by tube. Such a nutritional composition may be in various forms, for example, a fruit juice type beverage, a milk shake type beverage, or the like. The nutritional composition can also be a soluble powder that can be reconstituted before use.

  The nutritional composition can include various flavors (such as vanilla), sweeteners and other additives. Artificial sweeteners such as aspartame can be used.

5 to 500 mg (0.005 to 0.5%) of champignon extract with fecal odor reduction effect; 10 to 200 μg of carotenoid preparation (including α-carotene, β-carotene, lycopene, lutein, etc.) for the purpose of enhancing nutrition (0.00001 to 0.0002%) can also be included.
Furthermore, catechin, polyphenol, etc. can also be contained as an antioxidant.

  The nutritional composition can be produced, for example, by mixing proteins, lipids and carbohydrates at the blending ratio as described above. In this case, an emulsifier can be blended into the mixture.

  The nutritional composition of the present invention can be made into a product by a method known in the art. For example, after preliminarily heat-sterilizing the liquid nutritional composition and then aseptically filling the container (for example, a method using a combination of the UHT sterilization method and the aseptic packaging method), and after filling the liquid nutritional composition into the container, A method of sterilizing with a container (for example, an autoclave method).

  When the usage form is liquid, the homogenized product is filled in a can and sterilized by retort, or is again sterilized by heating at about 140 to 145 ° C. for about 5 to 8 seconds and then cooled and aseptically filled. When the use form is a powder, the homogenized product is spray-dried, for example. Moreover, when the usage form is solid, it can be solidified by adding agar or the like.

  In the field of neurosurgery, there are many patients with consciousness disorder that cannot take voluntary eating behavior, and in addition, middle-aged and older people over 40 years old often have some complications. In many cases, the digestive and absorptive capacity of these consciousness-impaired patients is not inhibited, and nutrition can be administered through the intestinal tract, which is a more physiological food intake route. Therefore, the role played by the nutritional composition of the present invention in terms of nutritional management is important. In addition, patients with multiple organ disorders (MODS) complicated with renal failure were prone to water / electrolyte abnormalities, which hindered early enteral nutrition. For such cases, liquid nutritional compositions that take into account water and electrolytes in renal failure are desired. The nutritional composition of the present invention can also be expected as such a nutritional composition.

  The nutritional composition of the present invention improves serum lipid metabolism of health functional foods (special health foods and nutritional functional foods) as well as uses as oral and tube nutrients, therapeutic foods, home foods for the sick It can also be used as a food displaying its action.

  Administration of the nutritional composition to the patient depends on the patient's condition, the patient's weight, the patient's age, and whether the nutritional composition is the only nutritional item, and so on. The dose is determined by the patient's doctor. If the nutritional composition is used as a supplement to other foods, the daily nutritional composition is reduced accordingly.

  The nutritional composition of the present invention can be taken, for example, by 2 to 5 multiple doses to supplement the necessary daily dose, or can be taken by a single dose. The nutritional composition can also be supplied continuously over the required period.

  Alternatively, agar can be added to the liquid nutritional composition, or water and agar can be added to the powdered nutritional composition and cooled and solidified after heat treatment. Since the solidified nutritional composition provides a feeling of fullness after ingestion, it can be ingested as an alternative to a normal solid food.

  EXAMPLES Hereinafter, although an Example and a test example demonstrate this invention concretely, this invention is not limited to these Examples.

Example 1
A liquid nutritional composition was prepared according to the blending amount of the raw materials shown in Table 1 below. This composition was 100 kcal / 100 mL, and the energy ratio was 23.7% protein, 30.2% lipid, and 46.1% carbohydrate. The energy ratio of oleic acid in the lipid was 70%, and the energy ratio of palatinose in the sugar was 69%. It was used as a nutritional composition in the following test examples.
Milk protein concentrate (MPC) is manufactured by New Zealand Fonterra, caseinate is manufactured by DMV, milk phospholipid is manufactured by Newzealand Dairy Ingredients Limited, indigestible dextrin is manufactured by Matsutani Chemical Industries, and high oleic sunflower oil is manufactured by Nippon Oil & Fats. Made by Nippon Oil & Fats Co., Ltd. (palmitic acid 6%, stearic acid 2%, oleic acid 19%, linoleic acid 12%, α-linolenic acid 60%), and palatinose is Shin Mitsui A product manufactured by Sugar Manufacturing Co., Ltd. was used.

Example 2
A liquid nutritional composition was prepared according to the blending amount of the raw materials shown in Table 2 below. The nutritional composition was 100 kcal / 100 mL and the energy ratio was 24% protein, 30% lipid, and 46% carbohydrate. The energy ratio of oleic acid in the lipid was 70%, and the energy ratio of palatinose in the sugar was 69%. It was used as a nutritional composition in the following test examples.

Example 3
A liquid nutritional composition was prepared according to the blending amount of the raw materials shown in Table 3 below. The nutritional composition was 100 kcal / 100 mL and the energy ratio was 22% protein, 30% lipid and 48% carbohydrate. The energy ratio of oleic acid in the lipid was 70%, and the energy ratio of palatinose in the sugar was 69%. It was used as a nutritional composition in the following test examples.

Example 4 (Preparation of nutritional composition powder)
53 kg of the liquid nutrient composition prepared according to the blending amount of the raw materials shown in Table 3 was concentrated to 32 kg with an evaporator. This concentrated nutrient composition was treated with a spray dryer (exhaust temperature 95 ° C., orifice N0 74, core N0 17) to obtain 10 kg of a nutritional composition powder. In addition, comparative control Maybalance C (Table 4) and Gruserna (Table 5) were similarly treated to obtain powder. The respective solid contents were 96.7% for the nutritional composition powder, 95.3% for Gurserna, and 96.3% for May Balance C. The energy per gram is 5.6 kcal for the nutritional composition powder, 5.5 kcal for Gurserna, and 4.6 kcal for Maybalance C powder.

Example 5 (Method for solidifying nutritional composition)
2 g of agar (trade name: Kanten Cook, manufactured by Ina Foods Co., Ltd.) was added to 120 g of the nutritional composition powder prepared in Example 4, and 150 mL of hot water (about 60 ° C.) was added and stirred. This was heat-treated for 5 minutes in a microwave oven (RE-BM5W SAMSUNG) having a 500 watt rated high-frequency output, and then solidified in a refrigerator. This nutritional composition is 672 kcal. The calorie of the nutritional composition can be adjusted with the required calorie, and the agar concentration is preferably 0.5 to 2%.

Test Example 1 (Influence on blood glucose level of normal rats)
A 19-week-old male Sprague-Dawley rat (Japan SLC Co., Ltd.) was purchased and the animal laboratory (specific-pathogen free, room temperature 23 ± 1 ° C, 12 hours light-dark cycle) at the University of Tokushima Animal Laboratory. Breeding was conducted according to the animal breeding regulations. One week after purchase, the powdered feed for breeding rats (MF type, manufactured by Oriental Yeast Co., Ltd.) was used for free consumption and free drinking water.
The day before the start of the experiment, after fasting overnight, 2.0 ml of blood was collected from the left jugular vein for biochemical examination under diethyl ether anesthesia, and the serum triglyceride (TG) concentration was measured. Thereafter, the rats were divided into 3 groups (n = 10) based on body weight and serum lipid concentration, and were used as MF group, MBC (Maybalance C) group, and test sample group, respectively. The composition of the test sample is shown in Tables 6 and 7. The experimental period was 8 weeks, and an MF diet, MBC diet, and test sample diet were given so that the food intake would be 70-80 kcal / day, and free drinking water was used. MBC and test samples were given as spray-dried powder for liquid food. Body weight was observed once a week and food consumption was observed daily. At the end of the test period, blood was collected in the same manner as before the start and the serum TG concentration was measured. The results are shown in Table 8.
Serum TG concentration hardly changed before and after breeding in the MF group, but increased significantly after breeding in the MBC group (p <0.001: Wilcoxson signed-ranks test). In contrast, a significant decrease (p <0.001: Wilcoxson signed-ranks test) was observed after breeding in the test sample administration group. Moreover, when the serum TG concentration after breeding was compared between the MBC group and the test sample administration group, the test sample administration group showed a significantly low value (p <0.05: Student's t-test).

  The nutritional composition of the present invention has an effect of improving serum lipid metabolism and is useful as an oral and enteral nutritional agent.

Claims (2)

A nutritional composition containing protein, lipid and carbohydrate, wherein the energy ratio is 10 to 25% protein, 10 to 35% lipid and 40 to 60% carbohydrate, and oleic acid in the energy ratio of lipid 60 to 90%, palatinose and / or trehalulose in the carbohydrate energy ratio is 60 to 100%, a nutritional composition for improving serum lipid metabolism. The nutrition composition of Claim 1 containing at least 1 sort (s) chosen from milk phospholipid, soybean lecithin, high oleic sunflower oil, and sesame oil.