JP2006098110A - Analyte bonding reaction method - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To bond a detection target material in a sample solution to a receptor on the bottom surface, in a biosensor kit used for examination, diagnosis or the like in an environment field, a food field or a medical field. <P>SOLUTION: In a constitution wherein the sample solution 6 and a labeling solution 9 are vibrated vertically to the upper surface of a working electrode 4, the detection target material 2 in the sample solution 6 and a detection target material capturing material 8 in the labeling solution 9 are brought into contact with the receptor 3 on the bottom surface and the detection object material 2, to be thereby bonded thereto. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention is a biosensor kit for inspection / diagnosis in the environmental field, food field, and medical field, which is used to rapidly detect a detection target, for example, a chemical substance that can be an antigen, an environmental hormone, a protein, a microorganism, or a virus. Analyte binding reaction method.

  Conventionally, as a method for binding reaction of a biological sample analyte in this type of biosensor kit, the method described in Patent Document 1 is known.

  Hereinafter, a method for binding reaction of the biological sample analyte in the biosensor kit will be described with reference to FIGS.

  As shown in the figure, a substrate 104 that forms a recess 103 in which a receptor 102 that specifically binds to a detection target substance 101 is solid-phased is fixed to a roll-type stirring device 105, and the detection target substance 101 is contained. The sample solution 106 is dropped into the recess 103 and the detection target substance 101 and the receptor 102 are combined while being stirred by the stirring device 105.

Next, washing is performed to remove excess detection target substance 101 that is not bound to the receptor 102. Next, a labeled solution 109 containing a detection target substance-capturing substance 108 that specifically binds to the detection target substance 101 labeled with the enzyme 107 is dropped into the recess 103, and the receptor is stirred with the stirring device 105. The detection target substance 101 and the detection target substance capturing substance 108 combined with 102 are combined. Next, washing is performed to remove excess detection target substance capturing substance 108 that is not bonded to the detection target substance 101. Next, a substrate 111 that reacts with the enzyme 107 to generate an enzyme reactive substance 110 is added, and the color or fluorescence of the enzyme reactive substance 110 is detected as intensity, and the detection target substance 101 is detected.
JP-A-10-108663

  In such a conventional analyte binding reaction method, since the stirring method is rolling, the detection target substance above the sample solution and the detection target substance capture substance above the label solution are immobilized on the bottom surface of the receptor. There is a problem that it cannot contact and bind to the analyte target substance that is bound to the receptor, and the detection target substance above the sample solution and the detection target substance capture substance above the label solution are solidified on the bottom surface. It is required to be able to come into contact with and bind to the receptor and the analyte target substance bound to the receptor.

  The present invention solves such a conventional problem, wherein the detection target substance above the sample solution and the detection target substance capture substance above the label solution are solidified on the bottom surface. It is an object of the present invention to provide an analyte binding reaction method capable of contacting and binding to a target receptor and the analyte target substance bound to the receptor.

  In the analyte binding reaction method of the present invention, in order to achieve the above object, the sample solution and the label solution are vibrated perpendicularly to the upper surface of the working electrode.

  By this means, the detection target substance above the sample solution and the detection target substance capture substance above the label solution are immobilized on the bottom surface of the receptor and the analyte target substance bound to the receptor An analyte binding reaction method is obtained that can be contacted and bound.

  Another means is that a sample solution containing a detection target substance and a labeled solution containing a detection target substance capturing substance that specifically binds to the detection target substance labeled with an enzyme are disposed on the upper surface of the working electrode. As a method of oscillating vertically, resonance is performed.

  By this means, the detection target substance above the sample solution and the detection target substance capture substance above the label solution are fixed on the bottom surface without the sample solution and the label solution jumping out of the liquid retaining container. There is obtained an analyte binding reaction method capable of contacting and binding the phased receptor and the analyte target substance bound to the receptor.

  Another means is that a sample solution containing a detection target substance and a labeled solution containing a detection target substance capturing substance that specifically binds to the detection target substance labeled with an enzyme are disposed on the upper surface of the working electrode. As a method of vertically vibrating and resonating, the substrate is vibrated and resonated.

  By this means, the sample solution and the labeling solution are not ejected from the liquid retaining container by vibrating and resonating with the substrate, and the detection target substance above the sample solution and the labeling solution are above the labeling solution. An analyte binding reaction method is obtained in which the detection target substance capturing substance can be brought into contact with and bound to the receptor immobilized on the bottom surface and the analyte target substance bound to the receptor.

  Another means is to provide springs on the front and back surfaces of the substrate as a method of vibrating and resonating the substrate.

  By this means, the detection target substance above the sample solution and the detection above the labeling solution are prevented without causing the sample solution and the labeling solution to jump out of the liquid retaining container by manually vibrating and resonating with the substrate. An analyte binding reaction method is obtained in which the target substance-capturing substance can be brought into contact with and bound to the receptor immobilized on the bottom surface and the analyte target substance bound to the receptor.

  In another method, as a method of vibrating and resonating the substrate, a magnetic material is formed on the substrate, a magnetic material is provided in a pair with the magnetic material, and the N and S poles of the magnetic material are alternately changed. .

  By this means, the sample solution and the labeling solution are not ejected from the liquid retaining container by vibrating and resonating with the substrate with magnetic characteristics, and the detection target substance and the detection solution above the labeling solution are located above the sample solution. An analyte binding reaction method is obtained in which the target substance-capturing substance can be brought into contact with and bound to the receptor immobilized on the bottom surface and the analyte target substance bound to the receptor.

  Another means is to vibrate and resonate the substrate by forming a charged portion obtained by charging a positive or negative charge on the substrate, providing an electrode so as to be paired with the charged portion, ,-Are changed alternately.

  By this means, the detection target substance above the sample solution and the detection above the labeling solution are prevented without causing the sample solution and the labeling solution to jump out of the liquid retaining container by vibrating and resonating with the substrate due to electrical characteristics. An analyte binding reaction method is obtained in which the target substance-capturing substance can be brought into contact with and bound to the receptor immobilized on the bottom surface and the analyte target substance bound to the receptor.

  In another method, as a method of vibrating and resonating the substrate, a deformed portion deformed by temperature is formed on the substrate, a Peltier element is provided so as to contact the deformed portion, and the Peltier element is alternately cooled and heated. It is supposed to be changed.

  By this means, the sample solution and the labeling solution can be oscillated and resonated with the material property deformed depending on the temperature so that the sample solution and the labeling solution above the sample solution do not jump out of the liquid retaining container. An analyte binding reaction method can be obtained in which the detection target substance capturing substance located above can be brought into contact with and bound to the receptor immobilized on the bottom surface and the analyte target substance bound to the receptor.

  Another means is that a stretchable film is formed on the working electrode, a receptor is fixed to the film, and the receptor is vibrated perpendicularly to the upper surface of the working electrode.

  By this means, only the receptor is vibrated, and the detection target substance above the sample solution and the detection target substance capture substance above the label solution are bound to the receptor immobilized on the bottom surface and the receptor. Thus, an analyte binding reaction method capable of contacting and binding to the analyte target substance is obtained.

  As another method for vibrating the receptor, a magnetic material is coupled to the receptor, a magnetic material is provided in a direction perpendicular to the upper surface of the working electrode, and the N and S poles of the magnetic material are alternately changed. It is what.

  By this means, only the receptor is vibrated by magnetic properties, the detection target substance above the sample solution and the detection target substance capture substance above the label solution are solid-phased on the bottom and the receptor Thus, an analyte binding reaction method capable of contacting and binding to the analyte target substance that is bound to the analyte is obtained.

  Another means is to vibrate the receptor by providing an electrode in a direction perpendicular to the upper surface of the working electrode, and alternately changing the electrode between + and-.

  By this means, only the receptor is vibrated with electrical characteristics, and the receptor having the detection target substance above the sample solution and the detection target substance capturing substance above the label solution immobilized on the bottom surface and the receptor Thus, an analyte binding reaction method capable of contacting and binding to the analyte target substance that is bound to the analyte is obtained.

  Another means is to bring the detection target substance and the detection target substance capturing substance close to the receptor.

  By this means, the detection target substance and the detection target substance capture substance are moved, and the detection target substance above the sample solution and the detection target substance capture substance above the label solution are immobilized on the bottom surface. And an analyte binding reaction method capable of contacting and binding to the analyte and the analyte target substance bound to the receptor.

  As another method, the sample solution and the labeling solution are discharged from the vicinity of the receptor by a dispenser that sucks and discharges the sample solution and the labeling solution as a method for bringing the detection target substance and the detection target substance capturing substance close to the receptor. It is intended to let you.

  By this means, the detection target substance and the detection target substance capture substance are moved manually only, and the detection target substance above the sample solution and the detection target substance capture substance above the label solution are fixed on the bottom surface. Thus, an analyte binding reaction method can be obtained which can contact and bind to the receptor to be bound and the analyte substance bound to the receptor.

  In another method, as a method of bringing the detection target substance and the detection target substance capturing substance close to the receptor, a magnetic substance is mixed in the sample solution and the labeling solution, and a magnetic body is provided in a direction perpendicular to the upper surface of the working electrode. To repel the magnetic body.

  By this means, the detection target substance and the detection target substance capture substance are moved by magnetic characteristics, and the detection target substance above the sample solution and the detection target substance capture substance above the label solution are solid-phased on the bottom surface. Thus, an analyte binding reaction method can be obtained which can contact and bind to the receptor to be bound and the analyte substance bound to the receptor.

  As another method, as a method for bringing the detection target substance and the detection target substance capturing substance close to the receptor, a Peltier element is provided in a direction perpendicular to the upper surface of the working electrode, and the Peltier element is higher than the liquid temperature of the sample solution and the label solution. It was decided to warm up.

  By this means, the detection target substance and the detection target substance capture substance are moved by temperature characteristics, and the detection target substance above the sample solution and the detection target substance capture substance above the label solution are solid-phased on the bottom surface. Thus, an analyte binding reaction method can be obtained which can contact and bind to the receptor to be bound and the analyte substance bound to the receptor.

  As another method, as a method of bringing the detection target substance and the detection target substance capturing substance close to the receptor, an electrode is provided in a direction perpendicular to the upper surface of the working electrode, and the electrode is set to + or-. .

  By this means, the detection target substance and the detection target substance capture substance are moved by electrical characteristics, and the detection target substance above the sample solution and the detection target substance capture substance above the label solution are solid-phased on the bottom surface. Thus, an analyte binding reaction method can be obtained which can contact and bind to the receptor to be bound and the analyte substance bound to the receptor.

  Another means is to generate bubbles from the working electrode as a method of bringing the detection target substance and the detection target substance capturing substance close to the receptor.

  By this means, the detection target substance and the detection target substance capture substance are moved by gas characteristics, and the detection target substance above the sample solution and the detection target substance capture substance above the label solution are solid-phased on the bottom surface. Thus, an analyte binding reaction method can be obtained which can contact and bind to the receptor to be bound and the analyte substance bound to the receptor.

  Another means is to rotate the sample solution and the labeling solution after they are dropped into the liquid retaining container as a method of bringing the detection target substance and the detection target substance capturing substance close to the receptor.

  By this means, the detection target substance and the detection target substance capture substance are moved by centrifugal force characteristics, and the detection target substance above the sample solution and the detection target substance capture substance above the label solution are fixed to the bottom surface. There is obtained an analyte binding reaction method capable of contacting and binding the phased receptor and the analyte target substance bound to the receptor.

  Another means is to provide an absorbent on a substrate other than the working electrode in the liquid retaining container as a method for bringing the detection target substance and the detection target substance capturing substance close to the receptor.

  By this means, the detection target substance and the detection target substance capture substance are moved by the absorption characteristics, and the detection target substance above the sample solution and the detection target substance capture substance above the label solution are fixed to the bottom surface. There is obtained an analyte binding reaction method capable of contacting and binding the phased receptor and the analyte target substance bound to the receptor.

  According to the present invention, the detection target substance above the sample solution and the detection target substance capture substance above the label solution are brought into contact with the receptor immobilized on the bottom surface and the analyte target substance bound to the receptor. It is possible to provide an effective analyte binding reaction method capable of binding.

  According to the first aspect of the present invention, there is provided a working electrode in which a receptor that specifically binds to a substance to be detected is solid-phased on a substrate, and a liquid retaining container formed around the working electrode, and the substance to be detected A detection target substance-capturing substance that specifically binds to the detection target substance labeled with an enzyme, wherein the detection target substance and the receptor are bound by dropping a sample solution containing The labeling solution contained is dropped into the retaining container, the detection target substance bound to the receptor and the detection target substance capture substance are combined, and then react with the enzyme to generate an enzyme reactive substance. In the method in which the enzyme reaction substance detects the detection target substance electrochemically as an electric signal by adding a substrate to be reacted, the sample solution and the labeling solution are shaken perpendicularly to the upper surface of the working electrode. The receptor, the analyte target substance, and the detection target substance capturing substance are bound to each other, and the detection target substance above the sample solution and the detection target above the label solution. The substance-capturing substance moves to the receptor solidified on the bottom surface by the vertical vibration and the analyte target substance bonded to the receptor, and as a result, can contact and bind.

  The invention according to claim 2 of the present invention includes a sample solution containing a detection target substance and a labeled solution containing a detection target substance capturing substance that specifically binds to the detection target substance labeled with an enzyme. As a method of oscillating perpendicularly to the upper surface of the working electrode, resonance is performed, and the sample solution and the labeling solution are not ejected from the liquid retaining container, and the detection above the sample solution is performed. The target substance and the detection target substance capturing substance located above the labeling solution can be brought into contact with and bound to the receptor immobilized on the bottom surface and the analyte target substance bound to the receptor. .

  In the invention according to claim 3 of the present invention, there is provided a sample solution containing a detection target substance and a labeled solution containing a detection target substance capturing substance that specifically binds to the detection target substance labeled with an enzyme. As a method of vibrating and resonating perpendicularly to the upper surface of the working electrode, the substrate is vibrated and resonated. By vibrating and resonating the substrate, the sample solution and the marker solution are removed from the liquid retaining container. Without being jumped out, the detection target substance above the sample solution and the detection target substance capture substance above the label solution are immobilized on the bottom surface of the receptor and the analyte target bound to the receptor It has the effect that it can contact and bind to a substance.

  According to a fourth aspect of the present invention, as a method of vibrating and resonating the substrate, springs are provided on the front and back surfaces of the substrate, and the sample is obtained by vibrating and resonating the substrate only by hand. A receptor in which a detection target substance above the sample solution and a detection target substance capture substance above the label solution are solidified on the bottom surface without causing the solution and the label solution to jump out of the retaining container and the receptor It has the effect | action that it can contact and couple | bond with the said test substance target substance couple | bonded with.

  According to a fifth aspect of the present invention, as a method of vibrating and resonating the substrate, a magnetic material is formed on the substrate, a magnetic material is provided in a pair with the magnetic material, and the N and S poles of the magnetic material are alternately changed. The sample solution and the label solution are not ejected from the liquid retaining container by vibrating and resonating with the substrate due to the magnetic characteristics, and the detection target substance and the label solution above the sample solution. The substance to be detected that is to be detected can be brought into contact with and bound to the receptor immobilized on the bottom surface and the analyte substance to be bound to the receptor.

  According to a sixth aspect of the present invention, as a method of vibrating and resonating the substrate, a charged portion obtained by charging a positive or negative charge is formed on the substrate, and an electrode is formed so as to be paired with the charged portion. The electrode solution is alternately changed between + and-, and the sample solution and the label solution are not ejected from the liquid retaining container by vibrating and resonating with the substrate due to electrical characteristics. The detection target substance above the label and the detection target capture substance above the label solution can be brought into contact with and bound to the receptor solid-phased on the bottom surface and the analyte target substance bound to the receptor. It has the action.

  According to a seventh aspect of the present invention, as a method of vibrating and resonating the substrate, a deformation portion that deforms due to temperature is formed on the substrate, a Peltier element is provided so as to contact the deformation portion, and the Peltier element is The cooling and warming are alternately changed, and the sample solution and the labeling solution do not jump out of the liquid retaining container by vibrating and resonating with the substrate with the material property that deforms depending on the temperature. The detection target substance above and the detection target substance capture substance above the label solution can be brought into contact with and bound to the receptor immobilized on the bottom surface and the analyte target substance bound to the receptor. Has an effect.

  According to an eighth aspect of the present invention, a stretchable film is formed on the working electrode, a receptor is fixed to the film, and the receptor is vibrated perpendicularly to the upper surface of the working electrode. Only the receptor is vibrated, and the detection target substance above the sample solution and the detection target substance capture substance above the label solution are bound to the receptor immobilized on the bottom surface and the receptor. It has the effect | action that it can contact and couple | bond with the said specimen object substance.

  According to a ninth aspect of the present invention, as a method of vibrating the receptor, a magnetic material is coupled to the receptor, a magnetic material is provided in a direction perpendicular to the upper surface of the working electrode, and the N and S poles of the magnetic material are provided. Are changed alternately, and only the receptor is vibrated by magnetic characteristics, and the detection target substance above the sample solution and the detection target substance capture substance above the label solution are fixed on the bottom surface. It has the effect of being able to contact and bind to the receptor that has been formed and the analyte target substance that is bound to the receptor.

  In the invention of claim 10 of the present invention, as a method of vibrating the receptor, an electrode is provided in a direction perpendicular to the upper surface of the working electrode, and the electrode is alternately changed between + and-. Only the receptor is vibrated by electrical characteristics, and the detection target substance above the sample solution and the detection target substance capture substance above the label solution are combined with the receptor immobilized on the bottom surface and the receptor. It has the effect | action that it can contact and couple | bond with the said specimen object substance.

  According to an eleventh aspect of the present invention, the detection target substance and the detection target substance capturing substance are brought close to a receptor, the detection target substance and the detection target substance capturing substance are moved, and the sample is moved. The detection target substance above the solution and the detection target substance capture substance above the label solution are brought into contact with and bound to the receptor immobilized on the bottom surface and the analyte target substance bound to the receptor. Has the effect of being able to.

  According to a twelfth aspect of the present invention, as a method for bringing the detection target substance and the detection target substance capturing substance closer to the receptor, the sample solution is introduced from the vicinity of the receptor by a dispenser that sucks and discharges the sample solution and the label solution. And the labeling solution is discharged, and the detection target substance and the detection target substance capturing substance are moved manually only, and the detection target substance and the label solution are above the sample solution. The detection target substance capturing substance can be brought into contact with and bound to the receptor solid-phased on the bottom surface and the analyte target substance bound to the receptor.

  According to a thirteenth aspect of the present invention, as a method of bringing the detection target substance and the detection target substance capturing substance close to the receptor, a magnetic substance is mixed in the sample solution and the labeling solution, and the perpendicular direction to the upper surface of the working electrode is obtained. A magnetic body is provided, and the magnetic body is repelled by the magnetic body, and the detection target substance and the detection target substance capture substance are moved by magnetic characteristics, and the detection target substance and the detection target located above the sample solution The detection target substance-capturing substance located above the labeling solution can be brought into contact with and bound to the receptor immobilized on the bottom surface and the analyte target substance bound to the receptor.

  According to a fourteenth aspect of the present invention, as a method of bringing the detection target substance and the detection target substance capturing substance close to the receptor, a Peltier element is provided in a direction perpendicular to the upper surface of the working electrode, and the Peltier element is used as a sample solution and The detection target substance and the detection target substance-capturing substance are moved by temperature characteristics, and the detection target substance and the label solution are located above the sample solution. It has an effect that a detection target substance capturing substance can be brought into contact with and bound to a receptor immobilized on the bottom surface and the analyte target substance bound to the receptor.

  According to a fifteenth aspect of the present invention, as a method of bringing the detection target substance and the detection target substance capturing substance close to the receptor, an electrode is provided in a direction perpendicular to the upper surface of the working electrode, and the electrode is set to + or-. The detection target substance and the detection target substance capturing substance are moved by electrical characteristics, and the detection target substance above the sample solution and the detection target substance capture substance above the label solution are moved to the bottom surface. It has the effect that it can contact and bind to the receptor solid-phased and the analyte substance bound to the receptor.

  According to the sixteenth aspect of the present invention, as a method of bringing the detection target substance and the detection target substance capturing substance close to the receptor, bubbles are generated from the working electrode. The substance and the substance to be detected to be detected are moved, and the substance to be detected above the sample solution and the substance to be detected above the label solution are bound to the receptor immobilized on the bottom surface and the receptor. It has the effect | action that it can contact and couple | bond with the said analyte object substance.

  Further, in the invention according to claim 17 of the present invention, as a method of bringing the detection target substance and the detection target substance capturing substance close to the receptor, the sample solution and the label solution are rotated after being dropped into the liquid retaining container. The detection target substance and the detection target substance capture substance are moved by centrifugal force characteristics, and the detection target substance above the sample solution and the detection target substance capture substance above the label solution are solid-phased on the bottom surface. It has the effect of being able to contact and bind to the receptor that has been formed and the analyte target substance that is bound to the receptor.

  In the invention according to claim 18 of the present invention, as a method for bringing the detection target substance and the detection target substance capturing substance close to the receptor, an absorbent is provided on a substrate other than the working electrode in the liquid retaining container. Yes, the detection target substance and the detection target substance capture substance are moved by absorption characteristics, and the detection target substance above the sample solution and the detection target substance capture substance above the label solution are solidified on the bottom surface. It has the effect | action that it can contact and couple | bond with the said to-be-analyzed substance and the said test substance target couple | bonded with the said receptor.

  Hereinafter, embodiments of the present invention will be described with reference to the drawings.

(Embodiment 1)
As shown in FIGS. 1, 2, 3, and 4, a working electrode 4 in which a receptor 3 that specifically binds to a detection target substance 2 is solid-phased on a substrate 1 and a liquid retaining container around the working electrode 4 5 is formed. The size of the working electrode 4 is in the range of 0.002 to 40 mm2. A sample solution 6 containing the detection target substance 2 is dropped into the liquid retaining container 5, and the sample solution 6 is vibrated perpendicularly to the upper surface of the working electrode 4 to detect the detection target substance 2 and the receptor. 3 is reacted and combined. Next, washing is performed to remove excess detection target substance 2 that is not bound to the receptor 3. Next, a label solution 9 containing a detection target substance capturing substance 8 that specifically binds to the detection target substance 2 labeled with the enzyme 7 is dropped into the liquid retaining container 5, and the label solution 9 is moved as described above. Vibrating perpendicularly to the upper surface of the electrode 4, the detection target substance 2 and the detection target substance trapping substance 8 bonded to the receptor 3 are reacted and combined. Next, washing is performed to remove excess detection target substance capturing substance 8 that is not bonded to the detection target substance 2. Next, a substrate 11 that reacts with the enzyme 7 to generate an enzyme reactive substance 10 is added, and the enzyme reactive substance 10 detects the detection target substance 2 as an electrical signal electrochemically. Here, as a method for detecting an enzyme reaction, a potentiostat 12 is connected to the working electrode 4, and a silver-silver chloride reference electrode 13 is installed in the substrate 11 solution in the liquid reservoir 5. Thus, the oxidation-reduction reaction of the substrate 11 by the enzyme 7 is electrochemically measured and detected.

  With the above configuration, the detection target substance 2 above the sample solution 6 and the detection target substance capture substance 8 above the label solution 9 are solidified on the working electrode 4 on the bottom by vertical vibration. The receptor 3 and the analyte target substance 2 bound to the receptor 3 can be moved and contacted to be bound.

  An example will be described below. A protein that is a marker for an infectious disease is used as the detection target substance 2. Moreover, the said board | substrate 1 is vibrated using the vibrator 14 which vibrates up and down as a vibration method. The results are shown in FIG. 4, but the reaction time is 60 min. In comparison, the detection sensitivity of the vertical vibration can be improved by a little less than twice that of the conventional rolling vibration. Thereby, the detection lower limit can be improved and the reaction time can be shortened.

  The material used for the substrate 1 is an insulating material such as glass or synthetic resin, and is formed to have an arbitrary thickness, so that the working electrode 4 material can be stably fixed. Any material that is not affected by the reagent solution such as the label solution 9 and the substrate 11 may be used.

  The materials of the receptor 3 and the detection target substance capturing substance 8 are not particularly described, but any biological material such as an antibody, a DNA / RNA probe, or a peptide may be used.

  The material used for the working electrode 4 is a conductive material such as noble metal such as gold or platinum, carbon or ceramics, and is a material that detects only a desired reaction with high sensitivity and is not easily influenced by other reactions. Anything is fine.

  Further, although the material for the liquid retaining container 5 is not particularly described, any material may be used as long as it is resistant to the sample solution 6, the labeling solution 9, and the substrate 11 such as PP (polypropylene).

  Further, the kind of the enzyme 7 is not particularly described, but any substance capable of producing the enzyme reaction substance 10 such as HRP (horseradish peroxidase) or PO (peroxidase) generally used in the ELISA method is used. Good.

  Further, the type of the substrate 11 is not particularly described, but any substrate having a redox action such as ferrocene or OPD (o-phenylenediamine) may be used.

  In addition, the detection target substance capturing substance 8 is interposed, but the enzyme 7 is labeled on the detection target substance 8 and mixed with the sample solution 6 to compete with and bind to the receptor 3. You may take the method.

  In addition, although no particular description has been made on the method of immobilizing the receptor 3 and the working electrode 4, any method that can immobilize the receptor 3 on the working electrode 4, such as a hydrophobic bond or an SS bond, can be used. Good.

  Moreover, although it describes that it detects electrochemically as a detection method, you may detect the said enzyme reaction material 10 as color development or fluorescence.

(Embodiment 2)
As shown in FIGS. 5 and 6, the second embodiment has substantially the same configuration as the first embodiment, and the same parts as those in the first embodiment are denoted by the same reference numerals and detailed description thereof is omitted. A sample solution 6 containing the detection target substance 2 and a labeled solution 9 containing a detection target substance capturing substance 8 that specifically binds to the detection target substance 2 labeled with the enzyme 7 are disposed on the upper surface of the working electrode 4. As a method of vibrating vertically, it is configured to resonate.

  In the above configuration, the detection target substance 2 above the sample solution 6 and the detection target substance above the label solution 9 without the sample solution 6 and the label solution 9 jumping out from the liquid retaining container 5. The capture substance 8 can be brought into contact with and bound to the receptor 3 immobilized on the working electrode 4 on the bottom surface and the analyte target substance 2 bonded to the receptor 3.

  An example will be described below. A protein that is a marker for an infectious disease is used as a substance to be detected. Moreover, the said board | substrate 1 is vibrated using the vibrator which vibrates up and down as a vibration method. The results are shown in FIG. 6, and 1 kHz is the best, and 400 Hz and 4 kHz before and after that are about half the sensitivity. This is because the detection target substance 2 above the sample solution 6 and the detection target substance capture substance 8 above the label solution 9 are solidified on the working electrode 4 on the bottom at 400 Hz. 3 and the analyte target substance 2 that is bound to the receptor 3 have few opportunities to contact and cannot be bound, resulting in a decrease in sensitivity. In addition, when the frequency is 4 kHz, the vertical vibration is complicated, and the sample solution 6 and the labeling solution 9 are not shaken, and the detection target substance 2 and the labeling solution 9 above the sample solution 6 are detected. Since the target substance capturing substance 8 cannot be bound without contact with the receptor 3 immobilized on the working electrode 4 on the bottom surface and the analyte target substance 2 bound to the receptor 3, the sensitivity is consequently reduced. Become. Therefore, it is possible to improve the sensitivity most when the sample solution 6 and the labeling solution 9 are vibrated up and down at the maximum without jumping out of the liquid retaining container 5, that is, resonating. Thereby, the detection lower limit can be improved and the reaction time can be shortened.

  The materials of the receptor 3 and the detection target substance capturing substance 8 are not particularly described, but any biological material such as an antibody, a DNA / RNA probe, or a peptide may be used.

  The material used for the working electrode 4 is a conductive material such as noble metal such as gold or platinum, carbon or ceramics, and is a material that detects only a desired reaction with high sensitivity and is not easily influenced by other reactions. Anything is fine.

  Further, although the material for the liquid retaining container 5 is not particularly described, any material may be used as long as it is resistant to the sample solution 6, the labeling solution 9, and the substrate such as PP (polypropylene).

  Further, the kind of the enzyme 7 is not particularly described, but any substance capable of producing the enzyme reaction substance 10 such as HRP (horseradish peroxidase) or PO (peroxidase) generally used in the ELISA method is used. Good.

  In addition, the detection target substance capturing substance 8 is interposed, but the enzyme 7 is labeled on the detection target substance 8 and mixed with the sample solution 6 to compete with and bind to the receptor 3. You may take the method.

  In addition, although no particular description has been made on the method of immobilizing the receptor 3 and the working electrode 4, any method that can immobilize the receptor 3 on the working electrode 4, such as a hydrophobic bond or an SS bond, can be used. Good.

(Embodiment 3)
As shown in FIG. 7, the third embodiment has substantially the same configuration as that of the first or second embodiment, and the same parts as those of the first or second embodiment are denoted by the same reference numerals and detailed description thereof is omitted. A sample solution 6 containing the detection target substance 2 and a labeled solution 9 containing a detection target substance capturing substance 8 that specifically binds to the detection target substance 2 labeled with the enzyme 7 are disposed on the upper surface of the working electrode 4. As a method of vibrating and resonating vertically, the substrate 1 is vibrated and resonated. The configuration for vibrating and resonating the substrate 1 will be described in detail in Embodiments 4, 5, 6, and 7. Here, the substrate 1 is configured to vibrate and resonate.

  In the above configuration, the sample solution 6 and the labeling solution 9 are not ejected from the liquid retaining container 5 by vibrating and resonating with the substrate 1, and the detection target substance 2 and the label above the sample solution 6 The detection target substance capturing substance 8 located above the solution 9 is brought into contact with and bound to the receptor 3 immobilized on the working electrode 4 on the bottom surface and the analyte target substance 2 bound to the receptor 3. it can.

  The material used for the substrate 1 is an insulating material such as glass or synthetic resin, and is formed to have an arbitrary thickness, so that the working electrode 4 material can be stably fixed. Any material that is not affected by the reagent solution such as the label solution 9 and the substrate 11 may be used.

  The materials of the receptor 3 and the detection target substance capturing substance 8 are not particularly described, but any biological material such as an antibody, a DNA / RNA probe, or a peptide may be used.

  The material used for the working electrode 4 is a conductive material such as noble metal such as gold or platinum, carbon or ceramics, and is a material that detects only a desired reaction with high sensitivity and is not easily influenced by other reactions. Anything is fine.

  Further, although the material for the liquid retaining container 5 is not particularly described, any material may be used as long as it is resistant to the sample solution 6, the labeling solution 9, and the substrate such as PP (polypropylene).

  Further, the kind of the enzyme 7 is not particularly described, but any substance capable of producing the enzyme reaction substance 10 such as HRP (horseradish peroxidase) or PO (peroxidase) generally used in the ELISA method is used. Good.

  In addition, the detection target substance capturing substance 8 is interposed, but the enzyme 7 is labeled on the detection target substance 8 and mixed with the sample solution 6 to compete with and bind to the receptor 3. You may take the method.

  In addition, although no particular description has been made on the method of immobilizing the receptor 3 and the working electrode 4, any method that can immobilize the receptor 3 on the working electrode 4, such as a hydrophobic bond or an SS bond, can be used. Good.

(Embodiment 4)
As shown in FIG. 8, the fourth embodiment has substantially the same configuration as the third embodiment, and the same parts as those of the third embodiment are denoted by the same reference numerals and detailed description thereof is omitted. As a method of vibrating and resonating the substrate 1, springs 15 are provided on the front and back surfaces of the substrate 1.

  In the above configuration, when the spring 15 is pushed, the substrate 1 vibrates and resonates due to the expansion and contraction of the spring 15. As a result, the sample solution 6 and the labeling solution 9 are caused to vibrate and resonate with the substrate 1 only manually. The detection target substance 2 above the sample solution 6 and the detection target substance capture substance 8 above the label solution 9 are solid-phased on the working electrode 4 on the bottom without jumping out of the liquid retaining container 5. Can be brought into contact with and bound to the receptor 3 that has been formed and the analyte 2 that is bound to the receptor 3.

    The material used for the substrate 1 is an insulating material such as glass or synthetic resin, and is formed to have an arbitrary thickness, so that the working electrode 4 material can be stably fixed. Any material that is not affected by the reagent solution such as the label solution 9 and the substrate 11 may be used.

  The materials of the receptor 3 and the detection target substance capturing substance 8 are not particularly described, but any biological material such as an antibody, a DNA / RNA probe, or a peptide may be used.

  The material used for the working electrode 4 is a conductive material such as noble metal such as gold or platinum, carbon or ceramics, and is a material that detects only a desired reaction with high sensitivity and is not easily influenced by other reactions. Anything is fine.

  Further, although the material for the liquid retaining container 5 is not particularly described, any material may be used as long as it is resistant to the sample solution 6, the labeling solution 9, and the substrate such as PP (polypropylene).

  In addition, the detection target substance capturing substance 8 is interposed, but the enzyme 7 is labeled on the detection target substance 8 and mixed with the sample solution 6 to compete with and bind to the receptor 3. You may take the method.

  In addition, although no particular description has been made on the method of immobilizing the receptor 3 and the working electrode 4, any method that can immobilize the receptor 3 on the working electrode 4, such as a hydrophobic bond or an SS bond, can be used. Good.

  Moreover, although it described that it was comprised so that the spring 15 might be provided in the front and back of the said board | substrate 1, either the front or the back may be sufficient, and anything may be used as long as it can expand and contract.

(Embodiment 5)
As shown in FIG. 9, the fifth embodiment has substantially the same configuration as the third embodiment, and the same parts as those of the third embodiment are denoted by the same reference numerals and detailed description thereof is omitted. As a method of vibrating and resonating the substrate 1, a magnetic body 16 is formed on the substrate 1, a magnetic body 17 is provided in a pair with the magnetic body 16, and the N and S poles of the magnetic body 17 are alternately changed. .

  In the above-described configuration, the sample solution can be obtained by causing the substrate 1 to vibrate and resonate by alternately changing the N and S poles of the magnetic body 17 so that an attractive force and a repulsive force act between the magnetic body 17 and the magnetic body 16. 6 and the label solution 9 do not jump out of the liquid retaining container 5, and the detection target substance 2 above the sample solution 6 and the detection target substance capture substance 8 above the label solution 9 The working electrode 4 can be contacted and bound to the receptor 3 immobilized on the working electrode 4 and the analyte target substance 2 bound to the receptor 3.

  The material used for the substrate 1 is an insulating material such as glass or synthetic resin, and is formed to have an arbitrary thickness, so that the working electrode 4 material can be stably fixed. Any material that is not affected by the reagent solution such as the label solution 9 and the substrate 11 may be used.

  The materials of the receptor 3 and the detection target substance capturing substance 8 are not particularly described, but any biological material such as an antibody, a DNA / RNA probe, or a peptide may be used.

  The material used for the working electrode 4 is a conductive material such as noble metal such as gold or platinum, carbon or ceramics, and is a material that detects only a desired reaction with high sensitivity and is not easily influenced by other reactions. Anything is fine.

  Further, although the material for the liquid retaining container 5 is not particularly described, any material may be used as long as it is resistant to the sample solution 6, the labeling solution 9, and the substrate such as PP (polypropylene).

  In addition, the detection target substance capturing substance 8 is interposed, but the enzyme 7 is labeled on the detection target substance 8 and mixed with the sample solution 6 to compete with and bind to the receptor 3. You may take the method.

  In addition, although no particular description has been made on the method of immobilizing the receptor 3 and the working electrode 4, any method that can immobilize the receptor 3 on the working electrode 4, such as a hydrophobic bond or an SS bond, can be used. Good.

  In addition, the magnetic body 16 is formed on the substrate 1, the magnetic body 17 is provided in a pair with the magnetic body 16, and the N and S poles of the magnetic body 17 are alternately changed. The magnetic body 17 may be formed on the substrate 1 and may be constituted by a pair of magnetic bodies as a result.

(Embodiment 6)
As shown in FIG. 10, the sixth embodiment has substantially the same configuration as that of the third embodiment, and the same parts as those of the third embodiment are denoted by the same reference numerals and detailed description thereof is omitted. As a method of vibrating and resonating the substrate 1, a charged portion 18 in which a positive or negative charge is charged is formed on the substrate 1, an electrode 19 is provided so as to be paired with the charged portion 18, and the electrode 19 is ,-Are alternately changed.

  In the above configuration, the sample solution 6 is generated by causing the substrate 1 to vibrate and resonate when the electrode 19 is alternately changed between + and-, thereby causing attraction and repulsion between the electrode 19 and the charged portion 18. And the labeling solution 9 does not jump out of the liquid retaining container 5, and the detection target substance 2 above the sample solution 6 and the detection target substance capturing substance 8 above the labeling solution 9 are moved on the bottom surface. The receptor 3 immobilized on the electrode 4 and the analyte target substance 2 bound to the receptor 3 can be contacted and bound.

  The material used for the substrate 1 is an insulating material such as glass or synthetic resin, and is formed to have an arbitrary thickness, so that the working electrode 4 material can be stably fixed. Any material that is not affected by the reagent solution such as the label solution 9 and the substrate 11 may be used.

  The materials of the receptor 3 and the detection target substance capturing substance 8 are not particularly described, but any biological material such as an antibody, a DNA / RNA probe, or a peptide may be used.

  The material used for the working electrode 4 is a conductive material such as noble metal such as gold or platinum, carbon or ceramics, and is a material that detects only a desired reaction with high sensitivity and is not easily influenced by other reactions. Anything is fine.

  Further, although the material for the liquid retaining container 5 is not particularly described, any material may be used as long as it is resistant to the sample solution 6, the labeling solution 9, and the substrate such as PP (polypropylene).

  In addition, the detection target substance capturing substance 8 is interposed, but the enzyme 7 is labeled on the detection target substance 8 and mixed with the sample solution 6 to compete with and bind to the receptor 3. You may take the method.

  In addition, although no particular description has been made on the method of immobilizing the receptor 3 and the working electrode 4, any method that can immobilize the receptor 3 on the working electrode 4, such as a hydrophobic bond or an SS bond, can be used. Good.

  In addition, the charged portion 18 charged with + or − charge is formed on the substrate 1, an electrode 19 is provided so as to be paired with the charged portion 18, and the electrode 19 is alternately changed between + and −. However, conversely, the electrode 19 may be formed on the substrate 1, and the charging portion 18 may be provided so as to be paired with the electrode 19.

(Embodiment 7)
As shown in FIG. 11, the seventh embodiment has substantially the same configuration as the third embodiment, and the same parts as those of the third embodiment are denoted by the same reference numerals and detailed description thereof is omitted. As a method of vibrating and resonating the substrate 1, a deformed portion 20 that is deformed by temperature is formed on the substrate 1, a Peltier element 21 is provided so as to be in contact with the deformed portion 20, and the Peltier element 21 is alternately cooled and heated. It is configured to be changed.

  In the above-described configuration, the deforming force that causes the deformation portion 20 to warp or return by alternately changing between cooling and warming of the Peltier element 21 works to cause the substrate 1 to vibrate and resonate, thereby causing the sample solution 6 and the The labeling solution 9 does not jump out of the liquid retaining container 5, and the detection target substance 2 above the sample solution 6 and the detection target substance capturing substance 8 above the labeling solution 9 are moved to the bottom of the working electrode 4. Can be brought into contact with and bound to the receptor 3 that is solid-phased and the analyte 2 that is bound to the receptor 3.

  The material used for the substrate 1 is an insulating material such as glass or synthetic resin, and is formed to have an arbitrary thickness, so that the working electrode 4 material can be stably fixed. Any material that is not affected by the reagent solution such as the label solution 9 and the substrate 11 may be used.

  The materials of the receptor 3 and the detection target substance capturing substance 8 are not particularly described, but any biological material such as an antibody, a DNA / RNA probe, or a peptide may be used.

  The material used for the working electrode 4 is a conductive material such as noble metal such as gold or platinum, carbon or ceramics, and is a material that detects only a desired reaction with high sensitivity and is not easily influenced by other reactions. Anything is fine.

  Further, although the material for the liquid retaining container 5 is not particularly described, any material may be used as long as it is resistant to the sample solution 6, the labeling solution 9, and the substrate such as PP (polypropylene).

  In addition, the detection target substance capturing substance 8 is interposed, but the enzyme 7 is labeled on the detection target substance 8 and mixed with the sample solution 6 to compete with and bind to the receptor 3. You may take the method.

  In addition, although no particular description has been made on the method of immobilizing the receptor 3 and the working electrode 4, any method that can immobilize the receptor 3 on the working electrode 4, such as a hydrophobic bond or an SS bond, can be used. Good.

  Moreover, although the said Peltier device 21 was described as an apparatus which cools and warms, what can only be heated, such as a heater and infrared rays, may be used.

  In addition, although it has been described that the Peltier element 21 is cooled / warmed, it may be either cool / warm.

(Embodiment 8)
As shown in FIG. 12, the eighth embodiment has substantially the same configuration as the first embodiment, and the same parts as those of the first embodiment are denoted by the same reference numerals and detailed description thereof is omitted. A stretchable film 22 is formed on the working electrode 4, and the receptor 3 is fixed to the film 22, and the receptor 3 vibrates perpendicularly to the upper surface of the working electrode 4. The configuration in which the receptor 3 is fixed to the membrane 22 and the receptor 3 vibrates perpendicularly to the upper surface of the working electrode 4 will be described in detail in the ninth and tenth embodiments. It is configured to vibrate perpendicularly to the upper surface of the working electrode 4.

  In the above configuration, only the receptor 3 is vibrated, and the detection target substance 2 above the sample solution 6 and the detection target substance capture substance 8 above the label solution 9 are solidified on the working electrode 4 on the bottom surface. It is possible to contact and bind to the receptor 3 that has been prepared and the analyte 2 that is bound to the receptor 3.

  The materials of the receptor 3 and the detection target substance capturing substance 8 are not particularly described, but any biological material such as an antibody, a DNA / RNA probe, or a peptide may be used.

  The material used for the working electrode 4 is a conductive material such as noble metal such as gold or platinum, carbon or ceramics, and is a material that detects only a desired reaction with high sensitivity and is not easily influenced by other reactions. Anything is fine.

  The detection target substance capturing substance 8 is interposed, but the detection target substance 8 is labeled with an enzyme, mixed with the sample solution 6, and competitively reacted with the receptor 3 to bind. You may take

  In addition, although no particular description has been made on the method of immobilizing the receptor 3 and the working electrode 4, any method that can immobilize the receptor 3 on the working electrode 4, such as a hydrophobic bond or an SS bond, can be used. Good.

(Embodiment 9)
As shown in FIG. 13, the ninth embodiment has substantially the same configuration as that of the eighth embodiment, and the same parts as those of the eighth embodiment are denoted by the same reference numerals and detailed description thereof is omitted. As a method of vibrating the receptor 3, a magnetic body 23 is coupled to the receptor 3, a magnetic body 24 is provided in a direction perpendicular to the upper surface of the working electrode 4, and the N and S poles of the magnetic body 24 are alternately changed. It is configured.

  In the above configuration, the magnetic body 24 is alternately changed between the N and S poles so that an attractive force and a repulsive force act between the magnetic body 24 and the magnetic body 23 to vibrate only the receptor 3, and the sample solution 6 The detection target substance 2 above and the detection target substance capture substance 8 above the label solution 9 are bound to the receptor 3 immobilized on the working electrode 4 on the bottom and the receptor 3. It can contact and bind to the analyte target substance 2.

  The materials of the receptor 3 and the detection target substance capturing substance 8 are not particularly described, but any biological material such as an antibody, a DNA / RNA probe, or a peptide may be used.

  The material used for the working electrode 4 is a conductive material such as noble metal such as gold or platinum, carbon or ceramics, and is a material that detects only a desired reaction with high sensitivity and is not easily influenced by other reactions. Anything is fine.

  The detection target substance capturing substance 8 is interposed, but the detection target substance 8 is labeled with an enzyme, mixed with the sample solution 6, and competitively reacted with the receptor 3 to bind. You may take

  In addition, although no particular description has been made on the method of immobilizing the receptor 3 and the working electrode 4, any method that can immobilize the receptor 3 on the working electrode 4, such as a hydrophobic bond or an SS bond, can be used. Good.

  In addition, although it has been described that the magnetic body 23 is bound to the receptor 3, the same effect can be obtained even when the magnetic body 23 is bound to the film 22.

(Embodiment 10)
As shown in FIG. 14, the tenth embodiment has substantially the same configuration as the eighth embodiment, and the same parts as those of the eighth embodiment are denoted by the same reference numerals and detailed description thereof is omitted. As a method of vibrating the receptor 3, an electrode 25 is provided in a direction perpendicular to the upper surface of the working electrode 4, and the electrode 25 is alternately changed between + and-.

  In the above configuration, by alternately changing the electrode 25 between + and −, attractive force and repulsive force act between the receptor 3 to vibrate only the receptor 3, and the detection target above the sample solution 6 Contact the substance 2 and the detection target substance capturing substance 8 above the label solution 9 with the receptor 3 immobilized on the working electrode 4 on the bottom surface and the analyte target substance 2 bound to the receptor 3 Can be combined.

  The materials of the receptor 3 and the detection target substance capturing substance 8 are not particularly described, but any biological material such as an antibody, a DNA / RNA probe, or a peptide may be used.

  The material used for the working electrode 4 is a conductive material such as noble metal such as gold or platinum, carbon or ceramics, and is a material that detects only a desired reaction with high sensitivity and is not easily influenced by other reactions. Anything is fine.

  The detection target substance capturing substance 8 is interposed, but the detection target substance 8 is labeled with an enzyme, mixed with the sample solution 6, and competitively reacted with the receptor 3 to bind. You may take

  In addition, although no particular description has been made on the method of immobilizing the receptor 3 and the working electrode 4, any method that can immobilize the receptor 3 on the working electrode 4, such as a hydrophobic bond or an SS bond, can be used. Good.

(Embodiment 11)
As shown in FIG. 15, the eleventh embodiment has substantially the same configuration as the first embodiment, and the same parts as those in the first embodiment are denoted by the same reference numerals and detailed description thereof is omitted. The detection target substance 2 and the detection target substance capture substance 8 are brought close to the receptor 3. The configuration for bringing the detection target substance 2 and the detection target substance capturing substance 8 close to the receptor 3 will be described in detail in Embodiments 12, 13, 14, 15, 16, 17 and 18, but here the detection is performed. The target substance 2 and the detection target substance capturing substance 8 are configured to approach the receptor 3.

  In the above configuration, the detection target substance 2 and the specimen target substance capture substance 8 are moved, and the detection target substance 2 above the sample solution 6 and the detection target substance capture substance 8 above the label solution 9 are moved to the bottom surface. The working electrode 4 can be brought into contact with and bound to the receptor 3 immobilized on the working electrode 4 and the analyte material 2 bound to the receptor 3.

  The materials of the receptor 3 and the detection target substance capturing substance 8 are not particularly described, but any biological material such as an antibody, a DNA / RNA probe, or a peptide may be used.

  The material used for the working electrode 4 is a conductive material such as noble metal such as gold or platinum, carbon or ceramics, and is a material that detects only a desired reaction with high sensitivity and is not easily influenced by other reactions. Anything is fine.

  The detection target substance capturing substance 8 is interposed, but the detection target substance 8 is labeled with an enzyme, mixed with the sample solution 6, and competitively reacted with the receptor 3 to bind. You may take

  In addition, although no particular description has been made on the method of immobilizing the receptor 3 and the working electrode 4, any method that can immobilize the receptor 3 on the working electrode 4, such as a hydrophobic bond or an SS bond, can be used. Good.

(Embodiment 12)
As shown in FIG. 16, the twelfth embodiment has substantially the same configuration as that of the eleventh embodiment, and the same parts as those of the eleventh embodiment are denoted by the same reference numerals and detailed description thereof is omitted. As a method for bringing the detection target substance 2 and the detection target substance capturing substance 8 close to the receptor 3, the sample solution 6 and the labeling solution are introduced from the vicinity of the receptor 3 with a dispenser 26 that sucks and discharges the sample solution 6 and the labeling solution 9. 9 is discharged.

  In the above configuration, since the dispenser 26 discharges the sample solution 6 and the labeling solution 9 from the vicinity of the receptor 3, as a result, the detection target substance 2 and the detection target substance capture substance 8 are moved only manually. The detection target substance 2 above the sample solution 6 and the detection target substance capture substance 8 above the label solution 9 are combined with the receptor 3 immobilized on the working electrode 4 on the bottom surface and the receptor 3. The specimen target substance 2 can be contacted and bound.

  The materials of the receptor 3 and the detection target substance capturing substance 8 are not particularly described, but any biological material such as an antibody, a DNA / RNA probe, or a peptide may be used.

  The material used for the working electrode 4 is a conductive material such as noble metal such as gold or platinum, carbon or ceramics, and is a material that detects only a desired reaction with high sensitivity and is not easily influenced by other reactions. Anything is fine.

  The detection target substance capturing substance 8 is interposed, but the detection target substance 8 is labeled with an enzyme, mixed with the sample solution 6, and competitively reacted with the receptor 3 to bind. You may take

  In addition, although no particular description has been made on the method of immobilizing the receptor 3 and the working electrode 4, any method that can immobilize the receptor 3 on the working electrode 4, such as a hydrophobic bond or an SS bond, can be used. Good.

  In addition, although it has been described that the sample solution 6 and the labeling solution 9 are discharged from the vicinity of the receptor 3 by the dispenser 26 that sucks and discharges the sample solution 6 and the labeling solution 9, the sample solution 6 and the labeling solution 9 are discharged. The marker solution 9 may be sucked and discharged again, and the number of times may be any number.

(Embodiment 13)
As shown in FIG. 17, the thirteenth embodiment has substantially the same configuration as that of the eleventh embodiment, and the same parts as those of the eleventh embodiment are denoted by the same reference numerals and detailed description thereof is omitted. As a method for bringing the detection target substance 2 and the detection target substance capturing substance 8 close to the receptor 3, a magnetic substance 27 is mixed in the sample solution 6 and the labeling solution 9, and a magnetic substance 28 is provided in a direction perpendicular to the upper surface of the working electrode 4. The magnetic body 27 is configured to repel the magnetic body 27.

  In the above configuration, a repulsive force acts between the magnetic body 28 and the magnetic body 27 to move the detection target substance 2 and the detection target substance capture substance 8, and to detect the detection target substance 2 above the sample solution 6 and The detection target substance-capturing substance 8 above the label solution 9 is brought into contact with and bound to the receptor 3 immobilized on the working electrode 4 on the bottom surface and the analyte target substance 2 bound to the receptor 3. it can.

  The materials of the receptor 3 and the detection target substance capturing substance 8 are not particularly described, but any biological material such as an antibody, a DNA / RNA probe, or a peptide may be used.

  The material used for the working electrode 4 is a conductive material such as noble metal such as gold or platinum, carbon or ceramics, and is a material that detects only a desired reaction with high sensitivity and is not easily influenced by other reactions. Anything is fine.

  The detection target substance capturing substance 8 is interposed, but the detection target substance 8 is labeled with an enzyme, mixed with the sample solution 6, and competitively reacted with the receptor 3 to bind. You may take

  In addition, although no particular description has been made on the method of immobilizing the receptor 3 and the working electrode 4, any method that can immobilize the receptor 3 on the working electrode 4, such as a hydrophobic bond or an SS bond, can be used. Good.

(Embodiment 14)
As shown in FIG. 18, the fourteenth embodiment has substantially the same configuration as the eleventh embodiment, and the same parts as those in the eleventh embodiment are given the same reference numerals and detailed description thereof is omitted. As a method for bringing the detection target substance 2 and the detection target substance capturing substance 8 close to the receptor 3, a Peltier element 29 is provided in a direction perpendicular to the upper surface of the working electrode 4, and the Peltier element 29 is used as a solution of the sample solution 6 and the label solution 9. It is configured to warm above the temperature.

  In the above configuration, since the liquid temperature in the vicinity of the Peltier element 29 rises, convection occurs in the direction of the working electrode 4 to move the detection target substance 2 and the detection target substance capture substance 8 and above the sample solution 6. The detection target substance 2 and the detection target substance capture substance 8 above the label solution 9 are in contact with the receptor 3 immobilized on the working electrode 4 on the bottom surface and the analyte target substance 2 bound to the receptor 3. Can be combined.

  The materials of the receptor 3 and the detection target substance capturing substance 8 are not particularly described, but any biological material such as an antibody, a DNA / RNA probe, or a peptide may be used.

  The material used for the working electrode 4 is a conductive material such as noble metal such as gold or platinum, carbon or ceramics, and is a material that detects only a desired reaction with high sensitivity and is not easily influenced by other reactions. Anything is fine.

  The detection target substance capturing substance 8 is interposed, but the detection target substance 8 is labeled with an enzyme, mixed with the sample solution 6, and competitively reacted with the receptor 3 to bind. You may take

  In addition, although no particular description has been made on the method of immobilizing the receptor 3 and the working electrode 4, any method that can immobilize the receptor 3 on the working electrode 4, such as a hydrophobic bond or an SS bond, can be used. Good.

  Moreover, although the said Peltier device was described as an apparatus which warms, what could be heated, such as a heater and infrared rays, may be used.

(Embodiment 15)
As shown in FIG. 19, the fifteenth embodiment has a configuration substantially equivalent to that of the eleventh embodiment, and the same parts as those of the eleventh embodiment are given the same reference numerals and detailed description thereof is omitted. As a method for bringing the detection target substance 2 and the detection target substance capturing substance 8 closer to the receptor 3, an electrode 30 is provided in a direction perpendicular to the upper surface of the working electrode 4, and the electrode 30 is set to + or-. .

  In the above configuration, when the electrode 30 is set to + or −, a repulsive force acts between the electrode 30 and the detection target substance 2 and the detection target substance capture substance 8, and the detection target substance 2 and the detection target substance The capture substance 8 is moved, and the detection target substance 2 above the sample solution 6 and the detection target substance capture substance 8 above the label solution 9 are immobilized on the working electrode 4 on the bottom and the receptor 3 and It can contact and bind to the analyte target substance 2 bound to the receptor 3.

  The materials of the receptor 3 and the detection target substance capturing substance 8 are not particularly described, but any biological material such as an antibody, a DNA / RNA probe, or a peptide may be used.

  The material used for the working electrode 4 is a conductive material such as noble metal such as gold or platinum, carbon or ceramics, and is a material that detects only a desired reaction with high sensitivity and is not easily influenced by other reactions. Anything is fine.

  The detection target substance capturing substance 8 is interposed, but the detection target substance 8 is labeled with an enzyme, mixed with the sample solution 6, and competitively reacted with the receptor 3 to bind. You may take

  In addition, although no particular description has been made on the method of immobilizing the receptor 3 and the working electrode 4, any method that can immobilize the receptor 3 on the working electrode 4, such as a hydrophobic bond or an SS bond, can be used. Good.

(Embodiment 16)
As shown in FIG. 20, the sixteenth embodiment has substantially the same configuration as the eleventh embodiment, and the same parts as those in the eleventh embodiment are given the same reference numerals and detailed description thereof is omitted. As a method for bringing the detection target substance 2 and the detection target substance capturing substance 8 close to the receptor 3, the bubbles 31 are generated from the working electrode 4.

  In the above configuration, the bubbles 31 collect above the sample solution 6 and the label solution 9 to move the detection target substance 2 and the detection target substance capture substance 8, and the detection target substance 2 above the sample solution 6 and the above The detection target substance capturing substance 8 above the labeling solution 9 can be brought into contact with and bound to the receptor 3 immobilized on the working electrode 4 on the bottom surface and the analyte target substance 2 bound to the receptor 3. .

  The materials of the receptor 3 and the detection target substance capturing substance 8 are not particularly described, but any biological material such as an antibody, a DNA / RNA probe, or a peptide may be used.

  The material used for the working electrode 4 is a conductive material such as noble metal such as gold or platinum, carbon or ceramics, and is a material that detects only a desired reaction with high sensitivity and is not easily influenced by other reactions. Anything is fine.

  The detection target substance capturing substance 8 is interposed, but the detection target substance 8 is labeled with an enzyme, mixed with the sample solution 6, and competitively reacted with the receptor 3 to bind. You may take

  In addition, although no particular description has been made on the method of immobilizing the receptor 3 and the working electrode 4, any method that can immobilize the receptor 3 on the working electrode 4, such as a hydrophobic bond or an SS bond, can be used. Good.

(Embodiment 17)
As shown in FIGS. 21 and 22, the seventeenth embodiment has a configuration substantially equivalent to that of the eleventh embodiment, and the same parts as those of the eleventh embodiment are denoted by the same reference numerals and detailed description thereof is omitted. As a method for bringing the detection target substance 2 and the detection target substance capturing substance 8 close to the receptor 3, the sample solution 6 and the label solution 9 are dropped into the liquid retaining container 5 and then rotated.

  In the above-described configuration, a centrifugal force generated by the rotation of the sample solution 6 and the labeling solution 9 acts to move the detection target substance 2 and the detection target substance capturing substance 8, and the detection target substance located above the sample solution 6 2 and the detection target substance-capturing substance 8 above the labeling solution 9 come into contact with and bind to the receptor 3 immobilized on the working electrode 4 on the bottom surface and the analyte target substance 2 bound to the receptor 3. be able to.

  The materials of the receptor 3 and the detection target substance capturing substance 8 are not particularly described, but any biological material such as an antibody, a DNA / RNA probe, or a peptide may be used.

  The material used for the working electrode 4 is a conductive material such as noble metal such as gold or platinum, carbon or ceramics, and is a material that detects only a desired reaction with high sensitivity and is not easily influenced by other reactions. Anything is fine.

  Further, although the material for the liquid retaining container 5 is not particularly described, any material may be used as long as it is resistant to the sample solution 6, the labeling solution 9, and the substrate such as PP (polypropylene).

  The detection target substance capturing substance 8 is interposed, but the detection target substance 8 is labeled with an enzyme, mixed with the sample solution 6, and competitively reacted with the receptor 3 to bind. You may take

  In addition, although no particular description has been made on the method of immobilizing the receptor 3 and the working electrode 4, any method that can immobilize the receptor 3 on the working electrode 4, such as a hydrophobic bond or an SS bond, can be used. Good.

(Embodiment 18)
As shown in FIG. 23, the eighteenth embodiment has substantially the same configuration as the eleventh embodiment, and the same parts as those in the eleventh embodiment are given the same reference numerals and detailed description thereof is omitted. As a method of bringing the detection target substance 2 and the detection target substance capturing substance 8 close to the receptor 3, the absorbent 32 is provided on the substrate 1 other than the working electrode 4 in the liquid retaining container 5.
In the above configuration, the force sucked into the absorbent 32 works to move the detection target substance 2 and the detection target substance capture substance 8, and above the detection target substance 2 and the labeling solution 9 above the sample solution 6. The detection target substance capturing substance 8 in contact with the receptor 3 immobilized on the working electrode 4 on the bottom surface and the analyte target substance 2 bound to the receptor 3 can be brought into contact with each other.

  The material used for the substrate 1 is an insulating material such as glass or synthetic resin, and is formed to have an arbitrary thickness, so that the working electrode 4 material can be stably fixed. Any material that is not affected by the reagent solution such as the label solution 9 and the substrate 11 may be used.

  The materials of the receptor 3 and the detection target substance capturing substance 8 are not particularly described, but any biological material such as an antibody, a DNA / RNA probe, or a peptide may be used.

  The material used for the working electrode 4 is a conductive material such as noble metal such as gold or platinum, carbon or ceramics, and is a material that detects only a desired reaction with high sensitivity and is not easily influenced by other reactions. Anything is fine.

  Further, although the material for the liquid retaining container 5 is not particularly described, any material may be used as long as it is resistant to the sample solution 6, the labeling solution 9, and the substrate such as PP (polypropylene).

  The detection target substance capturing substance 8 is interposed, but the detection target substance 8 is labeled with an enzyme, mixed with the sample solution 6, and competitively reacted with the receptor 3 to bind. You may take

  In addition, although no particular description has been made on the method of immobilizing the receptor 3 and the working electrode 4, any method that can immobilize the receptor 3 on the working electrode 4, such as a hydrophobic bond or an SS bond, can be used. Good.

  In the analyte binding reaction method according to the present invention, the detection target substance above the sample solution and the detection target substance capture substance above the label solution are bound to the receptor immobilized on the bottom surface and the receptor. The environmental field and the food field that can contact and bind to the sample target substance, and are used for the rapid detection of chemicals, environmental hormones, proteins, microorganisms and viruses that can be detected objects, for example, antigens It is useful as a biosensor kit for inspection and diagnosis in the medical field.

Sectional drawing of the structure of the analyte reaction coupling | bonding method of Embodiment 1 of this invention Sectional view of the configuration of the analyte reaction bonding method Sectional view of the configuration of the analyte reaction bonding method Comparison data diagram of the same analyte reaction binding method Sectional drawing of the structure of the analyte reaction coupling | bonding method of Embodiment 2 of this invention Comparison data diagram of the same analyte reaction binding method Sectional drawing of the structure of the analyte reaction coupling | bonding method of Embodiment 3 of this invention Sectional drawing of the structure of the analyte reaction coupling | bonding method of Embodiment 4 of this invention. Sectional drawing of the structure of the analyte reaction coupling | bonding method of Embodiment 5 of this invention Sectional drawing of the structure of the analyte reaction coupling | bonding method of Embodiment 6 of this invention Sectional drawing of the structure of the analyte reaction coupling | bonding method of Embodiment 7 of this invention Sectional drawing of the structure of the analyte reaction coupling | bonding method of Embodiment 8 of this invention. Sectional drawing of the structure of the analyte reaction coupling | bonding method of Embodiment 9 of this invention Sectional drawing of the structure of the analyte reaction coupling | bonding method of Embodiment 10 of this invention. Sectional drawing of the structure of the analyte reaction coupling | bonding method of Embodiment 11 of this invention Sectional drawing of the structure of the analyte reaction coupling | bonding method of Embodiment 12 of this invention. Sectional drawing of the structure of the analyte reaction coupling | bonding method of Embodiment 13 of this invention Sectional drawing of the structure of the analyte reaction coupling | bonding method of Embodiment 14 of this invention Sectional drawing of the structure of the analyte reaction coupling | bonding method of Embodiment 15 of this invention. Sectional drawing of the structure of the analyte reaction coupling | bonding method of Embodiment 16 of this invention. Sectional drawing of the structure of the analyte reaction coupling | bonding method of Embodiment 17 of this invention Sectional view of the configuration of the analyte reaction bonding method Sectional drawing of the structure of the analyte reaction coupling | bonding method of Embodiment 18 of this invention. Sectional view of the configuration of a conventional analyte reaction bonding method Sectional view of the configuration of the analyte reaction bonding method

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Substrate 2 Detection object substance 3 Receptor 4 Working electrode 5 Liquid retaining container 6 Sample solution 7 Enzyme 8 Detection object substance capture substance 9 Labeling solution 10 Enzyme reaction substance 11 Substrate 12 Potentiostat 13 Silver / silver chloride reference electrode 14 Exciter 15 Spring DESCRIPTION OF SYMBOLS 16 Magnetic body 17 Magnetic body 18 Charging site 19 Electrode 20 Deformation site 21 Peltier element 22 Film 23 Magnetic body 24 Magnetic body 25 Electrode 26 Dispenser 27 Magnetic body 28 Magnetic body 29 Peltier element 30 Electrode 31 Foam 32 Absorbent 101 Detection target substance 102 Receptor DESCRIPTION OF SYMBOLS 103 Recess 104 Substrate 105 Stirrer 106 Sample solution 107 Enzyme 108 Detection target substance capture substance 109 Label solution 110 Enzyme reaction substance 111 Substrate

Claims (18)

A working electrode in which a receptor that specifically binds to a detection target substance is solid-phased on a substrate and a liquid retaining container are formed around the working electrode, and a sample solution containing the detection target substance is retained in the liquid retaining A liquid drop container containing a labeling solution containing a detection target substance capturing substance that is dropped into a container to bind the detection target substance and the receptor and then specifically binds to the detection target substance labeled with an enzyme. The substance to be detected that is dropped into the receptor and bound to the receptor is bound to the substance to be detected and the substance to be detected is bound, and then a substrate that reacts with the enzyme to generate an enzyme reactive substance is added, and the enzyme reactive substance becomes electrochemical In the method of detecting the detection target substance as an electrical signal, the receptor solution is obtained by vibrating the sample solution and the labeling solution perpendicularly to the upper surface of the working electrode. And the sample substance and the analyte binding reactions wherein the to couple the detection target substance capturing material. The sample solution containing the detection target substance and the labeled solution containing the detection target substance capturing substance that specifically binds to the detection target substance labeled with the enzyme are oscillated perpendicularly to the upper surface of the working electrode. 2. The analyte binding reaction method according to claim 1, wherein the method resonates. The sample solution containing the detection target substance and the labeled solution containing the detection target substance capturing substance that specifically binds to the detection target substance labeled with the enzyme vibrate and resonate perpendicularly to the upper surface of the working electrode. 3. The analyte binding reaction method according to claim 1, wherein the substrate is vibrated and resonated. 4. The analyte binding reaction method according to claim 3, wherein a spring is provided on the front and back surfaces of the substrate as a method of vibrating and resonating the substrate. As a method of vibrating and resonating the substrate, a magnetic material is formed on the substrate, a magnetic material is provided in a pair with the magnetic material, and the N and S poles of the magnetic material are alternately changed to vibrate and resonate the substrate. The analyte binding reaction method according to claim 3. As a method of vibrating and resonating the substrate, a charged portion obtained by charging a positive or negative charge is formed on the substrate, an electrode is provided so as to be paired with the charged portion, and the electrode is alternately changed between + and-. The analyte binding reaction method according to claim 3, wherein: As a method of vibrating and resonating the substrate, the substrate is formed with a deformed portion that is deformed by temperature, a Peltier element is provided so as to contact the deformed portion, and the Peltier element is alternately changed between cooling and warming. The analyte binding reaction method according to claim 3. The analyte binding reaction method according to claim 1, wherein a stretchable film is formed on the working electrode, a receptor is immobilized on the film, and the receptor is vibrated perpendicularly to the upper surface of the working electrode. 2. A method of vibrating a receptor, wherein a magnetic material is coupled to the receptor, a magnetic material is provided in a direction perpendicular to the upper surface of the working electrode, and the N and S poles of the magnetic material are alternately changed. 8. The analyte binding reaction method according to 8. 9. The analyte binding reaction method according to claim 8, wherein as a method of vibrating the receptor, an electrode is provided in a direction perpendicular to the upper surface of the working electrode, and the electrode is alternately changed between + and-. The analyte binding reaction method according to claim 1, wherein the detection target substance and the detection target substance capturing substance are brought close to the receptor. As a method for bringing the detection target substance and the detection target substance capturing substance closer to the receptor, the sample solution and the labeling solution are discharged from the vicinity of the receptor with a dispenser that sucks and discharges the sample solution and the labeling solution. The analyte binding reaction method according to claim 11. As a method of bringing the detection target substance and the detection target substance capturing substance close to the receptor, a magnetic substance is mixed in the sample solution and the label solution, a magnetic body is provided in a direction perpendicular to the upper surface of the working electrode, and the magnetic body is repelled by the magnetic body. The analyte binding reaction method according to claim 11, wherein: As a method for bringing the detection target substance and the detection target substance capturing substance close to the receptor, a Peltier element is provided in a direction perpendicular to the upper surface of the working electrode, and the Peltier element is heated to a temperature higher than the liquid temperature of the sample solution and the label solution. The analyte binding reaction method according to claim 11. 12. The analyte according to claim 11, wherein the detection target substance and the detection target substance capturing substance are brought close to the receptor, and an electrode is provided in a direction perpendicular to the upper surface of the working electrode, and the electrode is set to + or-. Binding reaction method. 12. The analyte binding reaction method according to claim 11, wherein bubbles are generated from the working electrode as a method for bringing the detection target substance and the detection target substance capturing substance close to the receptor. 12. The analyte binding reaction method according to claim 11, wherein, as a method of bringing the detection target substance and the detection target substance capturing substance closer to the receptor, the sample solution and the labeling solution are dropped after being dropped into the liquid retaining container. 12. The analyte binding reaction method according to claim 11, wherein an absorbent is provided on a substrate other than the working electrode in the liquid retaining container as a method for bringing the detection target substance and the detection target substance capturing substance closer to the receptor.

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