JP2005312380A - Rumen bypass agent for ruminant, feed of the same and method for producing the rumen bypass agent - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To obtain a rumen bypass agent excellent in rumen bypass properties and elution properties in digestive organs after abomasum even as a preparation containing many kinds of biological active substances with high water solubility, and to provide a method for producing the rumen bypass agent, having a few production processes. <P>SOLUTION: The method for producing the rumen bypass agent comprises producing the biological active substances only with a granulation process of granulating the dissolution liquid of a protector containing lecithin, and a 12-22C linear or branched, saturated or unsaturated aliphatic monocarboxylic acid or its salt, at 50-90°C liquid temperature by a jet granulation method. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

The present invention relates to a rumen bypass agent for ruminants, a feed containing the rumen bypass agent, and a method for producing the rumen bypass agent.

In ruminants such as cattle, when biologically active substances such as vitamins and amino acids are administered, most of them are decomposed by microorganisms that live in the rumen of ruminants and are not absorbed by ruminants and are wasted. It has become. A rumen bypass agent that protects these biologically active substances from degradation by microorganisms in the lumen and protects the biologically active substances by digestion and absorption in the digestive tract below the fourth stomach (the fourth stomach and the small intestine). Is extremely useful as a nutrient or the like.

As a conventional technique, it is a conventional practice to obtain a lumen bypass agent by dispersing a biologically active substance in wax as a protective agent, animal / vegetable oil or fat or a cured product thereof, or coating a core containing the biologically active substance. I have been.

For example, as a method of dispersing the biologically active substance in the protective agent, a method of granulating after mixing the biologically active substance with the protective agent (see Patent Document 1), or melting the biologically active substance A melt granulation method (see Patent Document 2) in which a melt mixed and suspended in a protective agent is dropped on a belt is described.

As a method for coating a biologically active substance with a protective agent, a method is described in which a core containing a biologically active substance is formed and then coated with the protective agent (see Patent Documents 3 and 4).
JP 60-168351 A JP 58-175449 A JP-A-63-317053 JP-A-5-23114

However, the bypass agent obtained by the method of dispersing the biologically active substance does not show a sufficient bypass effect for the biologically active substance having high water solubility, and the bypass agent obtained by the coating method Although the agent exhibits the performance as a bypass agent, the step of obtaining the bypass agent is as follows: 1: nuclear forming step (extrusion of granules → preparation of granules into spherical granules), 2: coating step (coating on the core), etc. Multiple processes are required, and the manufacturing process is complicated and expensive. Furthermore, there are only a limited number of biologically active substances contained in the rumen bypass agent, and livestock farmers have been eager for a rumen bypass agent containing many types of amino acids, vitamins and the like.

For a rumen bypass agent, if the biologically active substance is a low water-soluble substance in a formulation in which the biologically active substance is dispersed and granulated in the protective agent, the content is effective if the content is 40% or less. In the case of water-soluble biologically active substances such as highly water-soluble lysine hydrochloride, the rumen bypass property can be achieved even if the content of the biologically active substance is 40% or less. However, an effective bypass agent could not be obtained.

An object of the present invention is a preparation in which a biologically active substance is dispersed and granulated in a protective agent. Among biologically active substances, water-soluble substances such as lysine hydrochloride, betaine, taurine and / or water-soluble vitamins are used. Even in the case of containing a biologically active substance, it is to obtain a rumen bypass agent that is excellent in rumen bypass properties of each biologically active substance and excellent in elution in the digestive tract after the fourth stomach, and further It is to provide a rumen bypass agent that can be supplied to livestock farmers at a low price by producing the rumen bypass agent by a method having few production processes.

As a result of earnest research on the above object, the present inventor has found that at least one substance selected from hardened animal fats, hardened vegetable fats, waxes and waxes as a protective agent, lecithin, and a straight chain having 12 to 22 carbon atoms. Dispersing and / or dissolving a biologically active substance in a melt adjusted to 50 to 90 ° C. containing one or more of chain or branched saturated or unsaturated aliphatic monocarboxylic acids or salts thereof The formulation obtained only by the granulation process of granulating by the injection granulation method in which the molten liquid for injection is injected into the air at a liquid temperature of 50 to 90 ° C. is extremely effective as a lumen bypass agent and has a low It was found that it could be supplied at a price.

Examples of the melt granulation method include a method in which the melt is cooled and solidified in air, oil, water, or on a belt, and granulated, and the method for obtaining the granulated product of the present invention includes a cured animal property. At least one substance selected from fats and oils, hardened vegetable fats and oils, waxes and waxes, and lecithin and a linear or branched saturated or unsaturated aliphatic monocarboxylic acid or salt thereof having 12 to 22 carbon atoms A protective agent containing one or more kinds is melted, and the melt is prepared as a melt of a protective substance kept at 50 to 90 ° C., and then a biologically active substance is uniformly mixed and dispersed therein. This is a method in which a melt for injection kept at ˜90 ° C. is obtained, and the melt for injection is sprayed in air with an injection type granulator, cooled, solidified and granulated.

In addition, as a method for obtaining a melt for injection, there is a method in which all raw materials containing biologically active substances are dissolved at 50 to 90 ° C. at a time to obtain a melt for injection, but the important point is that The temperature of the melt containing the active substance is not brought to a temperature exceeding 90 ° C., or the holding time exceeding 90 ° C. is made as short as possible to adjust the melt for injection. This is because the biologically active substance is likely to be decomposed at a temperature exceeding 90 ° C., and the obtained rumen bypass agent may not have a prescribed content.

Furthermore, this air injection method is considered to be a granulation method that cools and solidifies in water or oil, and there is no need for water drying or oil removal step that accompanies granulation, and the production process is extremely high. It was also found that the production method was low in production cost due to the small amount.

That is, the present invention relates to (1): at least one substance selected from hardened animal fats, hardened vegetable fats, waxes and waxes as a protective agent, and a straight chain or branched chain having lecithin and 12 to 22 carbon atoms. A melt for spraying, in which a biologically active substance is dispersed and / or dissolved in a melt adjusted to 50 to 90 ° C. containing one or more of saturated or unsaturated aliphatic monocarboxylic acids or salts thereof Rumen bypass agent for ruminants obtained by producing the liquid by spray granulation method in which the liquid is injected into the air at a liquid temperature of 50 to 90 ° C., (2): linear or minute having 12 to 22 carbon atoms A rumen bypass agent for ruminants according to claim 1, wherein the branched saturated or unsaturated aliphatic monocarboxylic acid or a salt thereof is stearic acid, (3): the protective agent is palm oil and / or rapeseed A hardened oil Or rumen bypass agent for ruminants according to claim 2, (4): containing at least taurine and / or betaine as a biologically active substance, ruminant according to claim 1, 2 or 3 Animal rumen bypass agent, (5): feed containing rumen bypass agent for ruminant of claim 1, claim 2, claim 3 or claim 4, (6): hardened animal fat and oil as a protective agent, hardened At least one substance selected from vegetable oils and fats, waxes and waxes, and one or more of lecithin and a linear or branched saturated or unsaturated aliphatic monocarboxylic acid or salt thereof having 12 to 22 carbon atoms, or Injection that injects a melt for injection in which a biologically active substance is dispersed and / or dissolved in a melt adjusted to 50 to 90 ° C. containing two or more kinds at a liquid temperature of 50 to 90 ° C. To manufacture by granulation (7) A linear or branched saturated or unsaturated aliphatic monocarboxylic acid having a carbon number of 12 to 22 or a salt thereof is stearic acid. The method for producing rumen bypass agent for ruminants according to claim 6, (8): The ruminant according to claim 6 or 7, wherein the protective agent is palm hardened oil and / or oilseed rapeseed oil. 9. A method for producing a rumen bypass agent for a ruminant, (9): containing at least taurine and / or betaine as a biologically active substance, rumen bypass for ruminant animals according to claim 6, 7 or 8 The present invention relates to a method for producing an agent.

A rumen bypass agent obtained by spray granulation of a molten liquid such as a protective substance containing a biologically active substance hardly dissolves even in a biologically active substance having a high water solubility in the lumen. In addition to being able to make a very good rumen bypass agent, where the biologically active substance elutes below the fourth stomach, the manufacture of the rumen bypass agent is very simple, dispersing the biologically active substance in the molten protective agent The rumen bypass agent could be obtained simply by melt granulating the melted liquid, and the production cost could be kept extremely low.

In the present invention, examples of the biologically active substance include one or a mixture of two or more selected from amino acids, vitamins, enzymes, carbohydrates, natural products and pharmaceuticals.

Specifically, amino acids include aminoacetic acid, alanine, arginine, lysine, sodium glutamate, methionine, tryptophan, threonine, valine, betaine, taurine, lysine hydrochloride and the like; amino acids such as vitamins Water-soluble vitamins such as C, vitamin B1, vitamin B2, vitamin B6, vitamin B12, calcium pantothenate, nicotinic acid, biotin, folic acid, etc .; as enzymes, protease agents, amylase agents, lipase agents, mixed enzymes, etc .; Examples of carbohydrates include starch, sucrose, and glucose; examples of natural products include fish meal, seaweed meal, blood meal, cereal meal, and bile powder; medicines include antibiotics and hormonal agents, and antibiotics include aminoglycosides As a system, kanamycin sulfate, etc. Vancomycin etc. as a peptide system, oxytetracycline as a tetracycline system, ampicillin as a penicillin system, erythromycin as a macrolide system, lincomycin as a lincomycin system, chloramphenicols, fosfomycins, etc. Includes estrogen, stilbestrol, hexestrol and the like.
The content of the biologically active substance is selected in the range of 1 to 50% by weight.

Protective agents include hardened vegetable oils such as hardened palm oil, hardened soybean oil, rapeseed hardened oil, castor hardened oil, hardened animal oils such as hardened beef tallow and hardened pork fat, and waxes such as carnauba wax and beeswax As the class, one or a mixture of two or more selected from natural wax, synthetic wax, paraffin wax and the like can be used. The amount used is selected in the range of 20 to 98% by weight, preferably 30 to 90% by weight.

Examples of the linear or branched saturated or unsaturated aliphatic monocarboxylic acid having 12 to 22 carbon atoms or a salt thereof include myristic acid, palmitic acid, stearic acid, oleic acid, linoleic acid, behenic acid, and salts thereof 1 type or 2 or more types of mixtures chosen from the above can be used, and the usage-amount is chosen in the range of 0.1-50 weight%, Preferably it is 1-40 weight%.

The lecithin in the protective agent of the present invention is selected in the range of 0.1 to 10% by weight, preferably 0.5 to 7% by weight.

Furthermore, if necessary, calcium carbonate, calcium phosphate, talc, etc. can be added as a specific gravity adjuster for the rumen bypass agent, and fat-soluble vitamins such as vitamin A, vitamin D, vitamin K can also be added. .

Further, the rumen bypass agent obtained by spray granulation can be uniformly mixed with raw materials such as defatted rice bran, wheat flour, dried okara, corn and / or fish meal with a mixing machine such as a ribbon mixer to obtain a feed.

Examples are described in detail below, but the present invention is not limited thereto.
Preparation of rumen bypass agent: 1200 g of methionine, 1650 g of rapeseed oil, 50 g of lecithin, 70 g of stearic acid, 30 g of palmitic acid, and then a mixture of rapeseed oil, lecithin and stearic acid is heated to 85 ° C. It was. A methionine was uniformly mixed and dispersed in this protective substance melt, and a melt for spraying kept at 85 ° C. was obtained. The melt for injection was injected into the air to obtain a lumen bypass agent solidified into a spherical shape of 0.1 to 3 mm.

Evaluation as a lumen bypass agent of the present invention was performed by the following method.
(Evaluation of the protection of the rumen) The ratio of the biologically active substance eluted when the rumen bypass agent was immersed in the Mc Dougall solution corresponding to the rumen juice and shaken for 16 hours to the amount of the substance in the preparation. evaluate. McDougall buffer: A buffer solution containing 7.43 g of sodium bicarbonate, 7.00 g of disodium phosphate, 12 hydrate, 0.34 g of sodium chloride, 0.43 g of potassium chloride, 0.10 g of magnesium chloride / hydrate, and 0.05 g of calcium chloride in 1000 mL of water. liquid.

(Evaluation of dissolution of the 4th stomach) After measuring the dissolution rate of the 1st stomach, the solid was filtered off, and the solid was immersed in the Clark-Lubs buffer corresponding to the 4th gastric juice and shaken for 2 hours. The ratio of the biologically active substance eluted to the amount of the substance in the preparation is evaluated. Clark-Lubs buffer: A buffer solution in which 3.73 g of potassium chloride and 2.1 mL of hydrochloric acid are dissolved in 1000 mL of water.

(Evaluation of dissolution of the small intestine) After measuring the dissolution rate of the 4th stomach, the solid was filtered off, and the solid was immersed in a buffer solution corresponding to small intestinal juice and shaken for 7 hours. Evaluation is based on the ratio of the active substance to the amount of substance in the formulation.
As a result of carrying out the above evaluation method for this rumen bypass agent, the gastric dissolution rate was 2%, the gastric dissolution rate was 23%, and the small intestine dissolution rate was 71%.

Preparation of rumen bypass agent: After weighing 1200 g of lysine hydrochloride, 1300 g of rapeseed oil, 30 g of lecithin, and 470 g of stearic acid, melt the rapeseed oil, add lecithin and stearic acid, and keep at 80 ° C. It was. A lysine hydrochloride was uniformly mixed and dispersed in this protective substance melt to obtain a jetting melt kept at 80 ° C. The melt for injection was injected into the air to obtain a lumen bypass agent solidified into a spherical shape of 0.1 to 3 mm. Evaluation as a lumen bypass agent of the present invention was carried out in the same manner as in Example 1. The evaluation results for this lumen bypass agent are shown in Table 1.

Preparation of rumen bypass agent: 150 g of lysine hydrochloride, 400 g of methionine, 110 g of vitamin B, 1750 g of rapeseed hardened oil, 300 g of hardened rapeseed oil, 90 g of lecithin, 300 g of stearic acid, and then melt rapeseed hardened oil and hardened palm oil And stearic acid was added, and the mixture was kept at 75 ° C. to obtain a melt of the protective substance. A methionine and vitamin B1 were uniformly mixed and dispersed in this protective substance melt to obtain a jetting melt kept at 75 ° C. The melt for injection was injected into the air to obtain a lumen bypass agent solidified into a spherical shape of 0.1 to 3 mm. Evaluation as a lumen bypass agent of the present invention was carried out in the same manner as in Example 1. The evaluation results for this lumen bypass agent are shown in Table 1.

Preparation of rumen bypass agent: Lysine hydrochloride 150g, methionine 150g, betaine 200g, nicotinic acid 50g, taurine 100g, palm hardened oil 2150g, lecithin 50g, stearic acid 150g, weighed palm hardened oil, lecithin and stearic acid mixture The mixture was heated to obtain a melt and then kept at 70 ° C. to obtain a melt of a protective substance. A lysine hydrochloride, methionine, betaine, nicotinic acid, and taurine were uniformly mixed and dispersed in the melt of this protective substance to obtain a jet melt that was kept at 70 ° C. The melt for injection was injected into the air to obtain a lumen bypass agent solidified into a spherical shape of 0.1 to 3 mm. Evaluation as a lumen bypass agent of the present invention was carried out in the same manner as in Example 1. The evaluation results for this lumen bypass agent are shown in Table 1.

(Comparative Example) A commercially available rumen bypass agent was evaluated in the same manner as in Example 1. The evaluation results are shown in Table 1.

As is apparent from Table 1, as shown in the examples of the present invention, the rumen bypass agent containing two or more water-soluble biologically active substances can also be used against the rumen animal's rumen gastric simulant. In particular, it showed excellent bypassing properties and extremely excellent dissolution properties for the simulated fluid after the 4th stomach.

That is, when the present invention is orally administered to ruminants, each biologically active substance bypasses the rumen, even in a lumen bypass agent containing a large number of highly water-soluble biologically active substances. It was eluted in the digestive tract after the stomach and became a very effective rumen bypass agent that was absorbed without ruin by ruminants. Furthermore, the production method of the lumen bypass agent is extremely low in production cost, and can be produced only by a production process in which a melt dispersed in a protective agent solution in which a biologically active substance is melted is melted and granulated. It was convinced that it would be able to supply cheaply and would contribute greatly to the industry, especially to the livestock industry.

Claims (9)

At least one substance selected from hardened animal fats, hardened vegetable fats, waxes and waxes as a protective agent, lecithin and straight-chain or branched saturated or unsaturated aliphatic monocarboxylic acids having 12 to 22 carbon atoms A liquid for injection, in which a biologically active substance is dispersed and / or dissolved in a liquid adjusted to 50 to 90 ° C. containing one or more of carboxylic acids or salts thereof, is a liquid at 50 to 90 ° C. Rumen bypass agent for ruminants obtained by producing by spray granulation method in which air is injected into air. The rumen bypass agent for ruminants according to claim 1, wherein the linear or branched saturated or unsaturated aliphatic monocarboxylic acid having 12 to 22 carbon atoms or a salt thereof is stearic acid. The rumen bypass agent for ruminants according to claim 1 or 2, wherein the protective agent is hardened palm oil and / or hardened rapeseed oil. The rumen bypass agent for ruminants according to claim 1, 2 or 3, wherein at least taurine and / or betaine is contained as a biologically active substance. Feed containing the rumen bypass agent for ruminants of claim 1, claim 2, claim 3 or claim 4. At least one substance selected from hardened animal fats, hardened vegetable fats, waxes and waxes as a protective agent, lecithin and straight-chain or branched saturated or unsaturated aliphatic monocarboxylic acids having 12 to 22 carbon atoms A liquid for injection, in which a biologically active substance is dispersed and / or dissolved in a liquid adjusted to 50 to 90 ° C. containing one or more of carboxylic acids or salts thereof, is a liquid at 50 to 90 ° C. A method for producing rumen bypass agent for ruminants, characterized in that it is produced by an injection granulation method in which air is injected into air. The method for producing a rumen bypass agent for ruminants according to claim 6, wherein the linear or branched saturated or unsaturated aliphatic monocarboxylic acid having 12 to 22 carbon atoms or a salt thereof is stearic acid. The method for producing a rumen bypass agent for ruminants according to claim 6 or 7, wherein the protective agent is hardened palm oil and / or hardened rapeseed oil. The method for producing a rumen bypass agent for ruminants according to claim 6, 7 or 8, wherein the biologically active substance contains at least taurine and / or betaine.