JP2004534512A5

JP2004534512A5 -

Info

Publication number: JP2004534512A5
Application number: JP2002545628A
Authority: JP
Filing date: 2001-11-27
Publication date: 2005-12-22
Anticipated expiration: 2021-11-27

Description

なおさらに、本発明の別の局面により、十分な量のスターター微生物（ラクトコッカス属細菌、大腸菌属細菌、ビフィドバクテリウム属細菌、ラクトバチルス属細菌、またはシュードモナス属細菌など）を増殖させ、そしてスターター微生物を、前記スターター微生物の生存性を室温において少なくとも２ヶ月間にわたって持続させるように選択される、植物抽出物の揮発性画分（ＶＦ）の少なくとも１つを含有する配合物に懸濁することによって発酵プロセス用のスターター物を調製する方法が提供される。 Still further, according to another aspect of the present invention, a sufficient amount of a starter microorganism (Lactococcus bacteria, E. coli bacteria, Bifidobacterium bacteria, Lactobacillus bacteria, Pseudomonas bacteria, etc.) is grown and The starter microorganism is suspended in a formulation containing at least one of the volatile fractions (VF) of the plant extract, selected to maintain the viability of said starter microorganism for at least 2 months at room temperature This provides a method of preparing a starter for a fermentation process.

実施例８
胃腸炎の入院患者における食物補助物（実施例４）およびコリバクテリン（大腸菌Ｍ−１７の乾燥配合物）の適用
入院を伴う胃腸炎を発症した６０名の患者群を、性別、年齢および胃腸炎症状の重篤度の類似する分布を有する３つの小グループに無作為に分けた。すべての患者には、再水和、ビタミンなどを含む正常な支持療法が施された。状態の重症度は、抗生物質による治療を必要としなかった。２０名の患者からなる第１グループには、１０ｍｌの食物補助物（実施例４）が、７日間にわたって１日に３回、食前３０分に与えられた。同じ数の第２グループには、コリバクテリン（Ｃｏｌｉｂａｃｔｅｒｉｎｕｍｓｉｃｃｕｍ）が製造者が勧めるようにして与えられた（１用量を、食前３０分に、１日に２回）。第３グループには、補助物が全く与えられなかった。
すべてのグループにおける胃腸炎の症状の正常化開始（日数）を記録し、以下の表２に示す。

Example 8
Application of food supplements (Example 4) and colibacterin (a dry formulation of E. coli M-17) in hospitalized patients with gastroenteritis: a group of 60 patients who developed gastroenteritis with hospitalization were sex, age and symptoms of gastrointestinal inflammation It was randomly divided into three small groups with similar distributions of severity of. All patients received normal supportive care, including rehydration and vitamins. The severity of the condition did not require antibiotic treatment. In a first group of 20 patients, 10 ml of food supplement (Example 4) was given 30 minutes before meals three times a day for 7 days. The same number of the second group was given Colybacterin (Colibacterinum siccum) as recommended by the manufacturer (one dose, 30 minutes before meals, twice daily). The third group received no supplements at all.
The onset of normalization (days) of symptoms of gastroenteritis in all groups was recorded and is shown in Table 2 below.

実施例１１
後期放射線全腸炎の患者における食物補助物（実施例４）の適用
食物補助物（１０ｍｌ、１４日間にわたって１日に３回、食前３０分）を、放射線療法または放射線と化学療法との組合せの後に全腸炎を発症した子宮ガン、結腸ガンおよび胃ガンの２４名の患者に与えた。
重量比が＞０．１であることを特徴とする像形成材料が提供される。 Example 11
Application of food supplements (Example 4) in patients with late radiation enterocolitis Food supplements (10 ml, 3 times a day for 14 days, 30 minutes before meals) after radiation therapy or a combination of radiation and chemotherapy It was given to 24 patients with uterine cancer, colon cancer and stomach cancer who developed enterocolitis.
An imaging element is provided, characterized in that the weight ratio is> 0.1.

実施例１２
ＡＩＤＳ患者における食物補助物（実施例４）の適用
ＡＩＤＳ罹患患者は慢性下痢を頻繁に発症する。そのような患者群に、１０ｍｌの食物補助物（実施例４）を、２０日間にわたって１日に３回、食前３０分に与えた。コントロール群には補助物を与えなかった。治療の結果を以下の表４に示す。

Example 12
Application of food supplements (Example 4) in AIDS patients AIDS sufferers frequently develop chronic diarrhea. In such a group of patients, 10 ml of food supplement (Example 4) was given 30 minutes before meals three times a day for 20 days. The control group received no supplements. The results of treatment are shown in Table 4 below.

実施例１５
下痢を示す子ブタにおける飼料添加物（実施例５）の適用
下痢を示す数百頭の子ブタに、毎日、１頭あたり５ｍｌの飼料添加物を経口投与した。同じサイズのコントロール群は抗生物質（アドボシン、ゲンチアマイシン、アモキシリン）で治療された。飼料添加物投与群における下痢症状は１日〜２日で消失した。死亡は認められず、子ブタは正常に発育した。抗生物質は、大多数の子ブタで下痢を止めたが、子ブタは、その発達において発達が妨げられたままであった。 Example 15
Application of feed additive (Example 5) in piglets showing diarrhea. Several hundred piglets showing diarrhea were orally administered 5 ml of feed additive per head daily. A control group of the same size was treated with antibiotics (advocin, gentiamycin, amoxycillin). The diarrhea symptoms in the feed additive administration group disappeared in 1 to 2 days. There were no deaths and piglets grew normally. Antibiotics stopped diarrhea in the majority of piglets, but piglets remained developmentally hindered in their development.

実施例１６
健康な子ウシにおける飼料添加物（実施例５）の適用
日齢の健康な子ウシに、１日あたり５ｍｌの飼料添加物をミルクにおいて７日間投与した。９５％を越える子ウシは、ロタウイルスについて少数の陽性例が診断された１４日齢になるまで下痢を発症しなかった。このファームにおける下痢の発現率は通常２０％〜３０％である。 Example 16
Application of Feed Additives (Example 5) in Healthy Calves Day-old healthy calves were dosed with 5 ml of feed additives per day in milk for 7 days. More than 95% of calves did not develop diarrhea until 14 days of age when a few positive cases for rotavirus were diagnosed. The incidence of diarrhea in this farm is usually 20% to 30%.

実施例１７
下痢を示す子ウシにおける飼料添加物（実施例５）の適用
下痢を発症する子ウシに、毎日、１頭あたり１０ｍｌの飼料添加物をミルクとともに３日間〜５日間投与した。下痢症状は、９０％〜９５％の子ウシにおいて１日〜２日で消失した。残る５％〜１０％では、下痢はウイルスにより生じていた。これらの子ウシは抗生物質で治療されたが、結果は不良であった。 Example 17
Application of feed additives (Example 5) in calves showing diarrhea Calves developing diarrhea were daily administered 10 ml of feed additive per cow with milk for 3 to 5 days. The diarrhea symptoms disappeared in 1 to 2 days in 90% to 95% calves. In the remaining 5% to 10%, the diarrhea was caused by the virus. These calves were treated with antibiotics but the results were poor.

実施例１８
健康な子ヒツジおよび子ヤギにおける飼料添加物（実施例５）の適用
１日齢の健康な子ヒツジおよび子ヤギに、１日あたり３ｍｌの飼料添加物をミルクにおいて７日間投与した。数例で、軽度〜普通の下痢徴候が認められた。これらの徴候は、通常は自然に消失し、または増量した飼料添加物（５ｍｌ）を用いて問題なく治療された。これらファームでの下痢の発現率は子ヒツジおよび子ヤギにおいて通常５０％である。 Example 18
Application of feed additives (Example 5) in healthy lambs and goats One day old healthy lambs and goats were dosed with 3 ml feed additive per day in milk for 7 days. Mild to normal signs of diarrhea were noted in several cases. These symptoms usually disappeared spontaneously or were successfully treated with increased feed supplements (5 ml). The incidence of diarrhea in these farms is usually 50% in lambs and goats.

Claims

Biologically pure E. coli strain BU-230-98 (ATCC Deposit No. 202226 (DSM 12799)).

A probiotic composition comprising, as an active ingredient, viable E. coli strain BU-230-98 (ATCC Deposit No. 202226 (DSM 12799)) and a formulation for maintaining the viability of said E. coli strain.

The probiotic composition of claim 2, further comprising at least one flavoring agent.

The probiotic composition of claim 2, wherein the formulation comprises at least one of the volatile fraction (VF) of a plant extract.

5. The probiotic composition of claim 4, wherein the volatile fraction is prepared by steam distillation of the plant extract under a pressure below atmospheric pressure and at a bath temperature not exceeding 38 ° C.

The probiotic composition of claim 4, wherein the plant extract is obtained from a plant organ selected from the group consisting of leaves, stems, roots and fruits.

5. The probiotic composition of claim 4, wherein the plant extract is obtained from a plant selected from the group consisting of vegetables and herbs.

8. The probiotic composition of claim 7, wherein the vegetables are selected from the group consisting of soybean, alfalfa, garlic, beet and cabbage.

The probiotic composition of claim 7, wherein the herb is selected from the group consisting of parsley, mint and dill.

5. The probiotic composition of claim 4, further comprising a bee nest product.

11. The probiotic composition of claim 10, wherein said bee nest product is propolis.

The probiotic composition of claim 2 identified to prevent or treat gastrointestinal infections or gastrointestinal abnormalities.

The probiotic composition of claim 2 identified to maintain or restore the normal gastrointestinal microflora.

The probiotic composition of claim 2 identified to prevent or treat diarrhea.

15. The probiotic composition of claim 14 identified to prevent or treat gastrointestinal infections caused by enteric pathogens.

16. The probiotic composition of claim 15, wherein the pathogen is a gram negative bacterium or a gram positive bacterium.

The probiotic composition of claim 2 comprising distilled water and volatile fractions of beet, dill and parsley.

The probiotic composition of claim 2 comprising distilled water and volatile fractions of beet, dill and grapefruit.

The probiotic composition of claim 2 identified to prevent or treat gastrointestinal salmonella infection.

3. The probiotic composition of claim 2 identified to prevent or treat infectious diarrhea, chronic diarrhea, or diarrhea resulting from antibiotic therapy or radiation or chemotherapy.

The probiotic composition of claim 2 identified for treating infectious diarrhea and comprising distilled water and volatile fractions of beet, dill and parsley.

Said infectious disease diarrhea is C.I. difficile, Salmonella spp. 22. The probiotic composition of claim 21, wherein the probiotic composition is caused by Shigella bacteria, Campylobacter bacteria, E. coli, Proteus bacteria, Pseudomonas bacteria, or Clostridial bacteria.

The probiotic composition of claim 2 identified to normalize the physiological activity of the gastrointestinal tract.

42. A formulation comprising at least one volatile fraction (VF) of a plant extract, said plant extract being obtained by water extraction, said volatile fraction being under pressure below atmospheric pressure and A formulation prepared by steam distillation of the botanical extract at a bath temperature not exceeding ° C.

25. The formulation of claim 24, wherein the plant extract is obtained from a plant organ selected from the group consisting of leaves, stems, roots and fruits.

25. The formulation of claim 24, wherein the plant extract is obtained from a plant selected from the group consisting of vegetables and herbs.

27. The formulation of claim 26, wherein the vegetables are selected from the group consisting of soybean, alfalfa, garlic, beet and cabbage.

27. The formulation of claim 26, wherein the herb is selected from the group consisting of parsley, mint and dill.

25. The formulation of claim 24, further comprising a bee nest product.

30. The formulation of claim 29, wherein said bee nest product is propolis.

An animal feed additive comprising at least one of the volatile fraction (VF) of a plant extract, wherein the animal given the feed additive gains more weight per given time compared to a control animal Animal feed additives to accelerate animal weight gain, said plant extract being obtained by water extraction, said volatile fraction does not exceed below atmospheric pressure and exceed 38 ° C. Animal feed additives prepared by steam distillation of the plant extract at bath temperature.

32. The animal feed additive of claim 31, comprising distilled water and volatile fractions of beet, dill and grapefruit.

A food additive comprising at least one of the volatile fraction (VF) of a plant extract, wherein said food additive-administered body has a greater weight per predetermined time compared to a control dosage body. A food additive for accelerating weight gain in a dosage form to increase body weight, wherein said plant extract is obtained by water extraction, said volatile fraction is at a pressure below atmospheric pressure and 38 ° C. Food additive prepared by steam distillation of the plant extract at a bath temperature not exceeding.

34. The food additive of claim 33, comprising distilled water and volatile fractions of beet, dill and parsley.

A probiotic composition,
(A) a therapeutically effective amount of at least one viable probiotic microorganism having biological or therapeutic activity in the gastrointestinal tract;
(B) at least one of the volatile fractions (VF) of plant extracts, said plant extracts being obtained by water extraction, said volatile fractions being under a pressure below atmospheric pressure and at 38 ° C. A probiotic composition comprising at least one volatile fraction (VF) of plant extract, prepared by steam distillation of said plant extract at a bath temperature not exceeding.

36. The probiotic composition of claim 35, further comprising at least one flavoring agent.

36. The probiotic composition of claim 35, wherein the probiotic microorganism is E. coli.

36. The probiotic composition of claim 35, wherein the probiotic microorganism is E. coli strain BU-230-98 (ATCC Deposit No. 202226).

36. The probiotic composition of claim 35, identified to prevent or treat gastrointestinal infections or gastrointestinal abnormalities.

36. The probiotic composition of claim 35, identified to maintain or restore a balanced gastrointestinal microbiota.

36. The probiotic composition of claim 35, identified to prevent or treat diarrhea.

36. The probiotic composition of claim 35, identified to prevent or treat gastrointestinal infections caused by enteric pathogens.

43. The probiotic composition of claim 42, wherein the pathogen is a gram negative bacterium or a gram positive bacterium.

43. The probiotic composition of claim 42 for treating dyspepsia symptoms.

43. The probiotic composition of claim 42 for stimulating an immune response in a patient suffering from an immune disorder.

46. The probiotic composition of claim 45, wherein said immune disorder results from immune response suppression therapy.

36. The probiotic composition of claim 35, comprising distilled water and volatile fractions of beet and dill.

36. The probiotic composition of claim 35, identified to prevent or treat gastrointestinal salmonella infection.

36. The probiotic composition of claim 35, wherein the probiotic composition is identified to prevent or treat infectious diarrhea, chronic diarrhea, or diarrhea resulting from antibiotic therapy or radiation or chemotherapy.

36. The probiotic composition of claim 35, identified to treat infectious diarrhea, comprising distilled water and volatile fractions of beet and dill.

Said infectious disease diarrhea is C.I. difficile, Salmonella spp. 51. The probiotic composition of claim 50, wherein the probiotic composition is caused by Shigella bacteria, Campylobacter bacteria, E. coli, Proteus bacteria, Pseudomonas bacteria, or Clostridial bacteria.

36. The probiotic composition of claim 35, identified to balance the physiological activity of the gastrointestinal tract.

Containing distilled water, the volatile fraction is prepared from alfalfa, soybean, beet, dill, mint, parsley and cabbage, the probiotic microorganism is E. coli strain BU-230-98 (ATCC Accession No. 202226) 36. The probiotic composition of claim 35.

A composition,
(A) at least one microorganism,
(B) at least one of the volatile fractions (VF) of plant extracts, said plant extracts being obtained by water extraction, said volatile fractions being under a pressure below atmospheric pressure and at 38 ° C. Prepared by steam-distilling the plant extract at a bath temperature not exceeding, wherein at least one of the volatile fractions (VF) of the plant extract is 10 ⁷ cells / ml of survival of the at least one microorganism of the above A composition comprising at least one of the volatile fractions (VF) of plant extracts, selected to last at least 2 months at room temperature.

The at least one microorganism is a group consisting of E. coli bacteria, Alcaligenes bacteria, Arthrobacter bacteria, Bifidobacterium bacteria, Lactobacillus bacteria, Lactococcus bacteria, Lactococcus bacteria, Nitrosomonas bacteria and Pseudomonas bacteria 55. The composition of claim 54 which is selected.

A method of preparing volatile fractions of plants, comprising
(A) grinding plant material to obtain plant biomass,
(B) mixing the plant biomass with water and stirring at ambient temperature, thereby obtaining a water extract of the plant biomass;
(C) steam distilling the water extract under a subatmospheric pressure and at a bath temperature not exceeding 38 ° C., thereby distilling volatile fractions of the extract, and (d) the steam Collecting the volatile fractions obtained by distillation.

57. The method of claim 56, wherein the plant material is obtained from plant leaves, stems, roots or fruits.

58. The method of claim 57, wherein the plant is a vegetable or a herb.

59. The method of claim 58, wherein the vegetable or herb is selected from the group consisting of soybean, alfalfa, garlic, beet, dill, mint, parsley, grapefruit and cabbage.

57. The method of claim 56, wherein the pressure is 5 mbar to 10 mbar.

Mixing at least one of the volatile fractions (VF) of the plant extract with a physiologically or veterinarily acceptable additive or carrier or diluent, said plant extract being obtained by aqueous extraction A method of preparing a therapeutic formulation, wherein the volatile fraction is prepared by steam distilling the plant extract under a pressure below atmospheric pressure and at a bath temperature not exceeding 38 ° C.

A method of growing E. coli strain BU-230-98 (ATCC deposit No. 202226 (DSM 12799)), wherein a starter culture of E. coli strain BU-230-98 (ATCC deposit No. 202226 (DSM 12799)) is grown Inoculating the medium and growing the E. coli strain to an optical density of 15-30 at 650 nm.

A method of producing a probiotic composition, which is capable of surviving E. coli strain BU-230-98 (ATCC Deposit No. 202226 (DSM 12799)), the viability of 10 ⁷ cells / ml of said E. coli strain, A method comprising suspending in a formulation to maintain at room temperature for at least 2 months.

The formulation comprises at least one of the volatile fraction (VF) of a plant extract, said plant extract being obtained by water extraction, said volatile fraction being under a pressure below atmospheric pressure and 64. The method of claim 63, wherein the method is prepared by steam distilling the plant extract at a bath temperature not exceeding <RTIgt;

65. The method of claim 64, wherein said plant extract is obtained from a plant organ selected from the group consisting of leaves, stems, roots and fruits.

65. The method of claim 64, wherein the plant extract is obtained from a plant selected from the group consisting of vegetables and herbs.

67. The method of claim 66, wherein the vegetable or herb is selected from the group consisting of soybean, parsley, mint, dill, alfalfa, garlic, beet and cabbage.

65. The method of claim 64, further comprising the addition of a honeybee nest product.

69. The method of claim 68, wherein said bee nest product is propolis.

65. The method of claim 64, wherein at least one of the volatile fractions is prepared from alfalfa, soy, beet, dill and mint.

A method of preparing a probiotic composition comprising: treating a therapeutically effective amount of at least one probiotic microorganism having biological or therapeutic activity in the gastrointestinal tract, a volatile fraction of plant extract Suspending in a formulation containing at least one of (VF), said plant extract is obtained by water extraction, said volatile fraction is under a pressure below atmospheric pressure and above 38 ° C. Method prepared by steam distillation of the plant extract at no bath temperature.

72. The method of claim 71, wherein said probiotic microorganism is E. coli.

73. The method of claim 72, wherein said probiotic microorganism is E. coli strain BU-230-98 (ATCC Deposit No. 202226).

72. The method of claim 71, wherein at least one of the volatile fractions comprises volatile fractions of alfalfa, soy, beet, dill, mint, parsley and cabbage.

A method of preparing a composition comprising a microorganism, to last for at least 2 months at room temperature at least one viable microorganism of 10 ⁷ cells / ml, volatile fraction of a plant extract (VF) at least Suspending at least one microorganism in the formulation comprising one, said plant extract is obtained by water extraction, said volatile fraction does not exceed a pressure below atmospheric pressure and does not exceed 38 ° C. A method prepared by steam distillation of the plant extract at bath temperature.

The at least one microorganism is a group consisting of E. coli bacteria, Alcaligenes bacteria, Arthrobacter bacteria, Bifidobacterium bacteria, Lactobacillus bacteria, Lactococcus bacteria, Lactococcus bacteria, Nitrosomonas bacteria and Pseudomonas bacteria 76. The method of claim 75, wherein the method is selected.

A dispenser of microorganisms comprising a reservoir and a dispensing mechanism connected to the reservoir, the reservoir being effective to sustain the viability of at least one microorganism for at least two months at room temperature, The at least one microorganism is contained in a formulation containing at least one of the volatile fractions (VF) of the plant extract, said plant extract being obtained by water extraction, said volatile fraction being at atmospheric pressure A dispenser prepared by steam distilling the plant extract under low pressure and at a bath temperature not exceeding 38 ° C.

78. The dispenser of claim 77, wherein the dispenser is air floating.

78. The dispenser of claim 77, wherein the dispenser is handheld.

78. The dispenser of claim 77, wherein the dispenser is designed to automatically dispense a predetermined volume.

81. The dispenser of claim 80, wherein the dispenser is designed to automatically dispense a predetermined volume periodically.

A method of biocontrol of an organism comprising the step of spraying at least one viable microbe for biocontrol of the organism in an endemic area, said at least one viable microbe at 10 ⁷ cells / ml Present in a formulation containing at least one of the volatile fraction (VF) of a plant extract, selected to sustain the viability of said at least one microorganism for at least 2 months at room temperature, The extract is obtained by water extraction and the volatile fraction is prepared by steam distilling the plant extract under a pressure below atmospheric pressure and at a bath temperature not exceeding 38 ° C.

A method of removing oil contamination comprising the step of spraying at least one microorganism having the ability to degrade oil into environmentally harmless compounds in a contaminated area, said at least one viable microorganism being 10 ⁷ Said plant extract being present in a formulation containing at least one volatile fraction (VF) of a plant extract, which sustains the viability of said at least one microbe of cells / ml for at least 2 months at room temperature The product is obtained by water extraction and the volatile fraction is prepared by steam distilling the plant extract under a pressure below atmospheric pressure and at a bath temperature not exceeding 38 ° C.

A method of restoring an organic gas degrading microbial population in a biofilter, comprising the step of applying to the filter at least one microorganism capable of degrading an organic gas into environmentally harmless compounds, said at least one microorganism comprising Present in a formulation containing at least one volatile fraction (VF) of a plant extract, which sustains the viability of said at least one microorganism of 10 ⁷ cells / ml for at least 2 months at room temperature, A plant extract is obtained by water extraction and the volatile fraction is prepared by steam distilling the plant extract under a pressure below atmospheric pressure and at a bath temperature not exceeding 38 ° C.

Food product supplemented with viable E. coli strain BU-230-98 (ATCC Deposit No. 202226 (DSM 12799)).

86. The food product of claim 85, comprising at least one substance obtained from milk.

87. The food product of claim 86, wherein the food product is selected from the group consisting of cheese and yoghurt.

A method of preparing a starter for a fermentation process, wherein a starter microorganism is grown in an amount sufficient to induce a fermentation process, and said starter microorganism is maintained at room temperature for at least 2 months at room temperature. Suspension in a formulation containing at least one of the volatile fractions (VF) of the plant extract, said plant extract being obtained by water extraction, said volatile fractions being A method prepared by steam distilling the plant extract under a pressure below atmospheric pressure and at a bath temperature not exceeding 38 ° C.