JP2003335622A - Skin care preparation for external use - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a skin care preparation for external use, having excellent moisture retention and a synergistically increased improving effect on chapped skin and skin aging symptoms such as wrinkles. <P>SOLUTION: This skin care preparation for external use has the excellent moisture retention and the synergistically increased improving effect on chapped skin and skin aging symptoms such as wrinkles and is obtained by including a Salicaceae plant extract and one or more kinds selected from usually utilized cell activators such as various galenical medicines, plant extracts, ceramides, yeast extracts, nucleic acids and hydroxy fatty acids. A water extract of Salix nigra L. is preferably used as the Salicaxeae plant extract. <P>COPYRIGHT: (C)2004,JPO

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention has a high moisturizing effect,
The present invention relates to a skin external preparation which synergistically improves the effects of improving skin aging symptoms such as wrinkles and the effect of improving rough skin. More specifically, it relates to a skin external preparation comprising a Salicaceae plant and one or more selected from cell activating agents in combination.

[0002]

2. Description of the Related Art Conventionally, due to aging, exposure to ultraviolet rays, etc., the stratum corneum of the skin, epidermal cells and dermal cells are damaged, the number of cells in the epidermis is reduced, the metabolism of epidermal cells is reduced, and the skin turns. It is known that the overspeed becomes slow, the hyaluronic acid is reduced in the dermis, and collagen and elastin are denatured, causing aging of the skin such as wrinkle formation and deterioration of elasticity and rough skin symptoms. In order to prevent or improve these processes, many skin external preparations have been proposed. Recently, reflecting the nature-oriented and plant-oriented, or in the plant extract components, components that suppress the decomposition of collagen and elastin, which is a constituent of the dermal matrix, hyaluronic acid, etc., or promote their production,
The search for components that activate epidermal cells and dermal fibroblasts is being actively conducted.

For example, as a component for activating epidermal cells, a parsley extract and a beech tree extract are used in combination (Japanese Patent Application Laid-Open No. HEI-1)
1-335257) and the like are disclosed. In addition, loquat extract (Japanese Patent Publication No. 5-17206), α-hydroxyacetic acid (Japanese Patent Application Laid-Open No. Hei 5)
-112422), sterol ester of α-hydroxy acid (JP-A-8-104632), 6-benzylaminopurine (JP-A-7-233037), specific ribonuclease (JP-A-7-309778), L-lysyl-L-glycyl -L-histidine (JP-A-7-316192), milk-derived fibroblast growth factor (JP-A-8-119867), oxidized coenzyme A (JP-A-8-175961), almond, dandelion, Sambucus nigra, senkyu, assembly, Sohakuhi, tounin, carrot, hop, starfish, yokinin (JP-A-10-36279)
As a collagen metabolism improving agent, a silicic acid-related substance (JP-A-7-188036), as a collagen synthesis promoter, a combination of glycine, proline and alanine (JP-A-7-194375), and as a hyaluronic acid production promoter. Is a low molecular weight fraction of bovine serum having a molecular weight of 5,000 or less (JP-A-8-239404) and yeast extract (JP-A-8-16).
3983) and the like are disclosed.

However, among the above-mentioned components having a cell activating effect, the action and effect are insufficient, and the stability is poor, so that it is effective when contained in the skin external preparation base. Some have had to contain significant amounts to be effective. In addition, it has unfavorable side effects or irritation, adversely affects the stability of the formulation, is difficult to mix with external preparations in terms of odor or color, and it is difficult to maintain a certain action and quality. There were many things.

[0005]

Therefore, in the present invention, it is an object of the present invention to obtain a skin external preparation having a high moisturizing effect and synergistically improving the skin aging symptoms such as wrinkles and the rough skin improving effect. did.

[0006]

[Means for Solving the Problems] Various investigations have been made to solve the above problems. As a result, Salicaceae plants,
It is excellent to use in combination with one or more kinds selected from commonly used cell activating agents such as various crude drugs, plant extracts, ceramides, yeast extracts, nucleic acids, and hydroxy fatty acids in a skin external preparation. The present invention has been completed based on the finding that a moisturizing effect can be obtained, a synergistic improvement of skin aging symptoms such as wrinkles and a rough skin improving effect can be obtained.

Regarding the effectiveness of Salicaceae plant extracts, the whitening effect of dihydromyricenan contained in leaves (JP-A-63-316711) and anti-mutagenic effect (JP-A-1-17532), and bark extraction It is already known that the histamine release-inhibiting effect of the product (JP-A-10-287582), the fibroblast activation effect of salicin contained in the bark and leaves in large amounts (JP-A-11-80002), and the like.

Regarding the cell activating agent, it has been blended in the skin external preparation for a long time in anticipation of the skin roughening improving effect, etc., but it is preferable to use the Salicaceae plant extract in combination with the cell activating agent. The effect of the present invention obtained by the above has not been suggested at all.

[0009]

BEST MODE FOR CARRYING OUT THE INVENTION Willow family ( Salica) used in the present invention
ceae ) plants include Chosenia arbutifolia (Pallas) A. Skvortz; Ch.
osenia bracteosa Nakai), cottonwood willow ( porcupine ,
Poplar) the genus of cottonwood (Populus) (Populus siebo
ldi Miq.), Dero ( Populus maxmowiczii He
nry), Populus alba
L.), American Poppy ( Populus deltoides Mars
h .; Populus monilifera Ait .; Populus angulata Ai
v.), populus euphratica (Populus euphratica Oliv.), Europe Black Mountain break-in (Populus nigra L.), Populus nigra (Populus nigra var. italica Koehne) , Europe mountain leveling (Populus tremula), Salix of Kasupiyanagi (Salix acutifolia L.) , European willow
(Salix alba L .; Salix aurea Salisb .), Salix (Itoyanagi) (Salix babylonica L.), wildcat willow (Bakkoyanagi) (Salix bakko Kimura), Akameyanagi (Salix chaenomeloides Kimura), Naga bus sallow
( Salix gilgiana Seemen.), Pussy willow (Enoco willow, river willow) ( Salix gracilistyla Miq.), Dogwood willow ( Salix integra Thunb.), Willow tree ( Salix j
aponica Thunb.), Salix kinuyanagi Kimur
a), Koryanagi ( Salix koriyanagi Kimura; Salix pur
purea var. japonica Nakai), Frisode willow ( Salix l
eucopithecia Kimura), Salix matsu
dana Koidz.), Japanese willow tree
( Salix nakamurana Koidz.), Salix nigr
a ), Salix purpurea L., Salix purpurea L., Salix sachalinensis Fr. Sch
m.), willow tree ( Salix reinii Fr. e)
t Sav.), Salix sieboldiana Bl., Salix viebalis L .; Salix longifolia
Lam.), Fox willow ( Salix vulpin )
a), Oobayanagi genus Oobayanagi (Toisusu urbaniana
Salix urbaniana ), etc. are exemplified. Among these, especially from the viewpoint of its antibacterial action, the European porcupine ( Pop
ulus tremula ), willow ( Salix alba L .; Sa
lix aurea Salisb.), Salix (Itoyanagi) (Sal
ix babylonica L.), black willow ( Salix nigra ), purple willow ( Salix purpurea L.)
It is preferred to use more than one plant, and most preferred is Salix nigra bark extract. The site for obtaining an extract from a Salicaceae plant is not particularly limited, but a bark, a leaf and a female flower are preferably used in view of its antibacterial action.

Extraction solvents for obtaining these Salicaceae plant extracts include water, ethanol, methanol, isopropanol, isobutanol, n-hexanol, methylamyl alcohol, 2-ethylbutanol,
Alcohols such as n-octyl alcohol, glycerin, ethylene glycol, ethylene glycol monomethyl ether, ethylene glycol monoethyl ether,
Propylene glycol, propylene glycol monomethyl ether, propylene glycol monoethyl ether, triethylene glycol, 1,3-butylene glycol, hexylene glycol and other polyhydric alcohols or their derivatives, acetone, methyl ethyl ketone, methyl isobutyl ketone, methyl-n-propyl Ketones such as ketones, esters such as ethyl acetate and isopropyl acetate,
One or more mixed solvents selected from polar solvents such as ethers such as ethyl ether, isopropyl ether, n-butyl ether, etc. can be preferably used, and phosphate buffered saline can also be used. However, it is not particularly limited. For the purpose of the present invention, a polar solvent is preferable from the viewpoint of antibacterial activity, and further, one or more mixed solvents selected from methanol, ethanol, 1,3-butylene glycol, and water, particularly water, may be used. It is preferably used as a solvent.

Examples of the extraction method include a method of immersing at room temperature, cooling or heating, extraction using a distillation method such as steam distillation, and a Salixaceae (raw Salicaceae ).
Examples of the method include a pressing method of pressing a plant to obtain an extract, and these methods are used alone or in combination of two or more kinds.

[0012] Without being limited especially Salicaceae (Salicaceae) the ratio of plant and solvent used in the extraction, solvent for Salicaceae (Salicaceae) Plant 1 0.5 to 1000
The weight is preferably 0.5 to 100 times, particularly in terms of extraction operation and efficiency. Further, the extraction temperature is conveniently in the range of room temperature to the boiling point of the solvent or less under normal pressure, and the extraction time is preferably in the range of 2 hours to 2 weeks, although it varies depending on the extraction temperature and the like.

The Salicaceae plant extract thus obtained may be used as it is, but it may be used for deodorization, decolorization, concentration, etc. within the range of not losing the antibacterial activity of the present invention. It may be used as a fractionated product by adding a purification operation or further using column chromatography or the like. These extracts, purified products, and fractions can be made into a dry solid by removing the solvent from them, and can be provided in the form of being solubilized in a solvent such as alcohol, or in the form of an emulsion. it can. These Salixaceae plant extracts may be used alone or in combination of two or more, and may be used in an amount of 0.00
It is preferable to add 1 to 20% by weight.

In the present invention, the above-mentioned Salixaceae ( Sali
caceae ) A plant extract is used in combination with one or more selected from cell activating agents in a skin external preparation base.

Examples of such cell activating agents include ceramides and ceramide-like structural substances, yeast extracts and yeast culture supernatants, extracts of crude drugs such as cucumber, Eucommia ulmoides, litchi and amanine, plants of the genus Asparagus , acavana ( Epilobium , Avocado ( Persea americ
ana Mill.), aloe (Aloe) plants of the genus, apricot (Prunus
armeniaca L. var. ansu Maxim.), Ginkgo ( Ginkgo )
biloba L.), beech ( Fagus japonica Maxim.), garlic ( Allium sativum L. f. pekinense Makin)
o), Panax ginseng CAMeyer, Chamomile ( Matricariachamomilla L.), Yellowfin ( Phellod )
endron amurense Rupr.) and its cognate plant, cucumber (Cucumis sativus L.), calendula (Calendula Arve
nsis L.), ghetto ( Alpinia speciosa (Wendl.) K.
Schum.), Shiitake mushroom ( Lentinus edodes Sing.), Cinnamon (Kiwi) ( Actinidia chinensis Planc
h.), horsetail ( Equisetum arvense L.), horse chestnut ( Aesculus hippocastanum L.), garlic ( Allium sativum L.), yarrow ( Ach )
illea aplina L.;. Achillea sibilica Ledeb ), assembly (Swertia japonica Makino), Stephania cepharantha Hayata (Stephania cepharantha Hayata), lettuce (lettuce) (Lactuca sativa L.), clintonia udensis (Clintonia
udensis Trautv. et Mey .; Clintonia borealis ),
Capsicum annuum L., Capsicum annuum L., Calendula officinalis L., Houttuynia
cordata Thunb.), Horse chestnut ( Aesculus turbinata Bl
ume), Bukryou (Matsuhodo) ( Poria co cos Wol
f), grape ( Vitis vinifera L.), beech ( Fagus cren )
ata Blume), Luffa cylindrica M.Roemen,
Safflower (Carthamus tinctorius L.), rosemary (Rosmarinus officinalis L.), oranges (Citrus) plants of the genus, Sapindus (Sapindus mukurossi Gaertn.), Purple (Lithospermum officinale L.var. Erythrorhizon
Maxim.), Eucalyptus genus plants, Lilium genus plants, Eriophorum L. genus plants extracts, hydroxy fatty acids and their salts and derivatives, nucleic acids and related substances, extracted from eggshell membranes Protein, sericin and its hydrolyzate, lactic acid bacterium extract,
Bifidobacteria extract, algae extract, bittern components, vitamin A, vitamin B _2, vitamin B ₆ such, nicotinic acid and its salts and derivatives, ergocalciferol, vitamin D such as cholecalciferol, vitamin H compound , Spleen extracts of mammals such as human and bovine, basic and acidic fibroblast growth factors, and epidermal growth factor are exemplified as preferable ones, and one or more of them are selected and used.

Ceramide is an N-acyl derivative of sphingosine or a similar base thereof. In addition to natural ceramide,
Phytosphigocin-containing ceramide, N- (hexadecyloxyhydroxypropyl) -N-hydroxyethylhexadecanamide, N-acylsulfigocin-containing ceramide, synthetic ceramides such as sphingomyelin and phytosphingosine, and ceramide-like compounds is there. Further, examples of the ceramide derivative include glucosylceramide, galactosylceramide, and the like, and these ceramide derivatives can also be produced by using a generally known method.

In the present invention, generally referred to as type 1
~ Extracted from natural products such as ceramides having various structures such as type 6, for example, N-stearoyl phytosphingosine (Ceramide III manufactured by Cosmo Farm), hydroxystearyl phytosphingosine (ceramide VI manufactured by Cosmo Farm), and ceramides derived from rice bran A thing can also be used.

These ceramides and ceramide-like structural substances may be used either individually or in combination of two or more, preferably 0.0001 to 20% by weight in the total composition, particularly 0.01 to 20% by weight. It is preferable to add 10% by weight.

As the yeast extract, an extract of yeast with a polar solvent, a yeast which has been lysed by autolysis, acid hydrolysis, enzymatic decomposition, or the like, or which has been filtered, or the lysate that has been dried, and then a polar solvent can be used. The extracted one can be used. Extract the Eremascus ( Eremascus )
Genus, Endomyces ( Endomyces ) and other yeast belonging to the family Endomycetaceae ( Endomycetaceae ), Schizosaccharomyces ( Schizosaccharomyces ), Nadsonia ( Nadsoni )
a) the genus, Sakkaromikodesu (Saccharomycodes) genus, Hanseniasupora (Hanseniaspora) genus, Wicker Hami A (Wickerhamia) genus Saccharomyces (Saccharomyces)
Sp., Kluyveromyces (Kluyveromyces) genus, Rodderomisesu (Lodderomyces) genus, Wingea (Wingea) genus,
Endomikopushisu (Endomycopsis) genus Pichia (Pichi
a) sp., Hansenula (Hansenula) spp., Pakisoren (Pach
ysolen) genus, Shiteromisesu (Citeromyces) genus Debaryomyces (Debaryomyces) genus Schwanniomyces (Sc
hwanniomyces) genus, Dekkera (Dekkera) genus Saccharomyces Romi Kopu cis (Saccharomycopsis) genus, Ripomisesu (Lipo
myces ) Saccharomycetacea ( Saccharomycetacea
e ) Yeast belonging to the family, Spermophthora
Genus, Eremoteshiumu (Eremothecium) genus, Kureburoteshiumu (Crebrothecium) genus, Ashubuya (Ashbya) genus,
Nematosupora (Nematospora) genus, meth Gerhard Nico Weir (Metschnikowia) genus, flag Diaz box (Coccidias
cus ) spermophthoraceae ( Spermophthoraceae )
Ascomycete yeast such as yeast belonging to the family is preferably used.

As the extraction solvent, in addition to water, lower alcohols such as methanol, ethanol, propanol and isopropanol, polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, dipropylene glycol and glycerin, diethyl ether and dipropyl. It is possible to use ethers such as ether, esters such as ethyl acetate and butyl acetate, and polar organic solvents such as ketones such as acetone and ethyl methyl ketone.
One kind or two or more kinds are selected and used. Also, saline
Phosphate buffer, phosphate buffered saline, etc. may be used. The yeast may be freeze-dried and / or pulverized before being subjected to extraction, or may be homogenized in an extraction solvent or ultrasonically disrupted. It is also possible to irradiate the medium with ultraviolet rays to obtain an extract. The extraction temperature is suitably about 0 ° C. to a temperature not higher than the boiling point of the extraction solvent. The extraction time varies depending on the type of the extraction solvent and the extraction temperature, but it is suitable to be about 1 hour to 5 days.

The yeast culture supernatant is cultured at 15 ° C. to 28 ° C. for about 3 days to 4 weeks using a yeast culture medium such as MY medium, then the culture supernatant is collected and filtered through a membrane filter. Get it.

The extract or culture supernatant of the above-mentioned polar solvent of yeast can be contained in the external preparation for skin according to the present invention as it is, but it can be concentrated and dried, or the concentrated and dried solid can be mixed with water or It may be used after being redissolved in a polar solvent, or subjected to a purification treatment such as decolorization, deodorization, desalting or the like or a dispersion treatment within a range that does not impair the physiological action. Also for storage,
It is also possible to freeze-dry after the purification treatment and dissolve in a solvent before use. It can also be contained in a vesicle such as a liposome or a microcapsule.

The yeast extract or culture supernatant may be prepared as described above, or may be commercially available as "yeast extract" for pharmaceuticals and cosmetics. In the present invention, one or more selected from these yeast extract and yeast culture supernatant are used.
The content per total amount of the external preparation for skin is not particularly limited, but is preferably about 0.0001 to 10.0% by weight.

[0024] The herbal Kakkonekisu (Puerariae Radix) is a perennial plant belonging to the legume family (Leguminosae) kudzu (Puera
ria lobata Ohwi) root excluding the pericardium, made in Japan,
You can use Korean and Chinese square brackets, board brackets and powder brackets.

The herb Medicinal herb is parasitic on the insects of the order Lepidoptera and Coleoptera or its larvae to form sclerotia in its body, and is a child formed on the body surface of the host insect or its larvae in the summer. It is an entity, Cordyceps sinensis , and Cordycep s sobo
lifera B.), Pleurotus ostreatus ( Cordyceps militaris Lin)
k), Mimitake mushroom ( Cordyceps nutans Pat.) and the like can be used.

The herbal medicine Ganoderma lucidum ( Ganoderm)
Ganod ( Ganod ), a basidiomycete belonging to Ataceae
erma lucidum (Leyss. ex Fr.) Karst) is a dried fruiting body, and white sardine lacquer , Kazuno lacquer, etc. can be used.

The herbal medicine Alinin ( Lini Semen ) is an annual herb of Linaceae, Linum usitatis.
imum L.) seeds.

[0028] asparagus (Asparagus) plant of the genus is a perennial belonging to the Liliaceae (Liliaceae), which is MotoHara plant of herbal "Tenmondou" Kusasugikazura (Asparagus co
chinchinensis Merrill) In addition, willow batten Monde cormorant (Asparagus falcatus L.), asparagus asparagoides (Aspar
agus medeoloides Thunb.), Tachiten Mondow ( Aspar
agus myriocladus Hort.), Dutch Pheasant Beetle ( Aspa
ragus officinalis L.) etc. are illustrated. Flowers, leaves, stems,
Although various parts such as roots and fruits can be used, it is preferable to use roots or stems and foliar branches.

The Red-Nosed (Epilobium) plant of the genus is a perennial belonging to the Red-Nosed (Onagraceae) plant of the family, it is a MotoHara plant of herbal "Koukaishi" Yanagiran (Chamaenerion a
ngustifolium (L.) Scop .; Epilobium angustifolium
L.), as well as Acavana ( Epilobium pyrricholophum Fr. e.
t Sav.), Spodoptera litura ( Epilobium cephalostigm )
a ), Red scabbard ( Epilobium fauriei ), Red scabbard ( Epilobium foucaudianum ), Red scabbard ( Ep
ilobium palustre), Epirobiumu-Rachio Folie um (Epilobium latiofolium) and the like. Flowers, leaves,
Each of these parts such as stems, roots and fruits can be used, but it is preferable to use roots or stems and foliar branches.

Avocado ( Persea americana Mill.)
A tree belonging to the Lauraceae family, which can use various parts such as flowers, leaves, branch trunks, and fruits, but preferably fruits.

The plant of the genus Aloe belongs to the family Liliac.
eae ) is a woody succulent plant and is a herbal medicine "Aloe" ( Alo
e ) Used as a base plant. Aloe Ferokusu (Aloe ferox Mill.), Aloe Africana (Aloe a
fricana Mill.), Aloe spicata Bake
r), Aloe succotrina Lam.,
Aloe plicatilis Mill.,
Aloe bainesii Th. Dyer., Aloe marlothii Bgr., Aloe
Perry ( Aloe perryi Baker), Aloe Vera ( Aloe ve)
ra L.), Aloe barbadensis Mil
l.) and Kidachi Aloe ( Aloe arborescens Mill., Aloe
arborescens Mill. var. natalensis Berg.) and the like, and each part of leaves, flower stems, flowers and the like and the whole plant can be used, but it is preferable to use the leaf part.

Apricot ( Prunus armeniaca L. var. Ansu
Maxim.) Is a deciduous small tree belonging to the family Rosaceae , and can use various parts such as flowers, leaves, branches, fruits, and seeds, but it is preferable to use fruits and seeds.

Ginkgo biloba L. is a dioecious deciduous tree belonging to the family Ginkgoaceae .
Each part of leaves, branches, bark, flowers, seeds, and the whole tree can be used, but it is preferable to use leaves or seeds.

The dog beech ( Fagus japonica Maxim.) And beech ( Fagus crenata Blume) belong to the family Beech ( Fagaceae ).
In the deciduous tree belonging to the above category, leaves, branches, bark, flowers, fruits and the like, and whole trees can be used, but it is preferable to use leaves or bark or shoots.

Allium sativum L. f. Pek
inense Makino) and garlic ( Allium sativu)
m L.) is a perennial plant belonging to the lily family ( Liliaceae ) and is a basic plant of the herbal medicine “Taisan ( Allii Bulbus )”.
Although various parts such as leaves, stems, and stems can be used, it is preferable to use stems.

Panax ginseng CA Me
yer) is a perennial belonging to Araliaceae ,
It is the base plant of the herbal medicine " Ginseng Radix ". Various parts such as flowers, stems, leaves, roots can be used,
It is preferable to use roots.

[0037] Chamomile ( Matricaria chamomilla L.)
Is an annual or perennial plant belonging to the family Asteraceae ( Compositae ), and is a basic plant of the herbal medicine "Chamomillae Flos ". Each part of leaves, stems, roots, head flowers and the like and the whole plant can be used, but it is preferable to use head flowers.

Yellowfin ( Phellellodendron amurense Rupr.)
Is a dioecious deciduous tree belonging to the citrus family ( Rutaceae ), and is a base plant of the herbal medicine " Phaerodendri Cortex ". The same genus plant, Oobanokihada (Phel
lodendron amurense var. japonica Ohwi), Japanese oyster fly ( Phellodendron amurense var. lavallei Spra)
g.), Hirohaki Hada ( Phellodendron amurense var. sa
chalinense Fr. Schmidt.), yellow skin ( Phellodendron c
hinense Schneid.). Each part of these flowers, leaves, stems, bark, roots, drupes, etc. can be used.
It is preferable to use bark.

Cucumber ( Cucumis sativus L.) is an annual vine plant belonging to the family Cucurbitaceae . Its leaves, stems,
Although it is possible to use each part of flowers, fruits, etc. and whole grass,
Preference is given to using fruits.

Calendula arvensis L. and Calendula officinalis L. are first-year or second-year herbs belonging to the family Asteraceae ( Compositae ), and each part of leaves, stems, flowers, roots, and whole plants. Can be used, but it is preferable to use flowers.

The ghetto ( Alpinia) used in the present invention
speciosa (Wendl.) K. Schum.) is a ginger family ( Zing
As a perennial plant belonging to iberaceae ), each part such as leaves, stems, flowers and roots can be used, but it is preferable to use leaves, roots or flowers.

Lentinus edodes Sing.
Mushrooms belonging to the Agaricaceae family,
Stems and whole can be used.

Cinnamon Pear (Kiwi) ( Actinidia chin
ensis Planch.) is an evergreen vine plant belonging to the Actinidiaceae family, and its various parts such as flowers, leaves, stems and fruits can be used, but it is preferable to use fruits.

Horsetail ( Equisetum arvense L.) is a perennial fern belonging to the family Equisetaceae , and can be used for each part of rhizome, spores, vegetative stem, and whole plant, but whole plant can be used. preferable.

Horse chestnut ( Aesculus hippocastan
um L.) and horse chestnut ( Aesculus turbinata Blume)
Is a deciduous tree belonging to the family Hippocastanaceae , and can be used for each part such as leaves, branches, bark, flowers, fruits and whole trees, but it is preferable to use leaves, bark or fruits.

Achillea aplina
L.; Achillea sibilica Ledeb.) Is an Asteraceae ( Composi
Tae ) is a perennial plant that can be used in various parts such as flowers, leaves and stems, but it is preferable to use whole plants.

Assembly ( Swirtia japonica Makino)
Is a perennial plant belonging to the Gentianaceae family and is the base plant of the herbal medicine " Sewrtiae Herba ".
Although various parts such as flowers, leaves and stems can be used, it is preferable to use whole grass.

Stephania cepharan
tha Hayata) is a perennial vine plant belonging to the family Menispermaceae , and can be used for each part of leaves, flowers, stems, roots, seeds, etc., but it is preferable to use roots.

Chisha (lettuce) ( Lactuca sativa L.)
Is a perennial herb belonging to the family Asteraceae ( Compositae ).
Although various parts such as leaves and stems can be used, it is preferable to use leaves.

Swallow Omoto ( Clintonia udensis Traut
v. et Mey.; Clintonia borealis ) is a lily family ( Lilia
ceae ) is a perennial herb that is the base plant of the herbal medicine " Raikou Nana". Each part such as flower, leaf, stem can be used,
It is preferable to use leaves.

Capsicum annuum L. is a shrub-like woody perennial plant belonging to the Solanaceae family and is the base plant of the herb medicine " Capsici Fructus ". Although various parts such as flowers, leaves, stems and fruits can be used, it is preferable to use fruits.

Calendula officinalis
L.) is an annual plant or 2 belonging to Compositae
Each part of the annual plant such as flowers, leaves, stems and fruits can be used, but it is preferable to use flowers.

Dokudami ( Houttuynia cordata Thunb.)
Is a perennial plant belonging to the family Saururaceae and is the base plant of the herbal medicine " Houttuyniae Herba ". Each part such as flower, leaf, stem can be used,
It is preferable to use whole plants before flowering.

Bukryou ( Poria cocos Wo)
lf) belongs to the family Polyporaceae , and is a basic plant of the herbal medicine " Hoelen ". In the present invention, it is preferable to use sclerotia from which most of the outer layer has been removed.

Grape ( Vitis vinifera L.) is a deciduous vine plant belonging to the family Vitaceae , and includes flowers, leaves,
Although various parts such as stems, fruits and seeds can be used, it is preferable to use seeds.

Luffa cylindrica M. Roemen
Is an annual vine plant belonging to the family Cucurbitaceae, which can use flowers, leaves, stems, fruits, fruit juices, sap, etc., but preferably stems or fruits, fruit juices, sap .

Safflower ( Carthamus tinctorius L.)
Is an annual or perennial plant belonging to the family Asteraceae ( Compositae ), and is a basic plant of the herbal medicine " Carhami Flos ". Although various parts such as flowers, leaves, stems and seeds can be used, it is preferable to use flowers.

Mannenrou ( Rosmarinus officinalis
L.) is an evergreen shrub belonging to Labiatae and has leaves,
Each part of the branch, bark, flower, etc. and the whole tree can be used, but it is preferable to use leaves.

Citrus plants belong to the Rutaceae family ( Ru
taceae ) is an evergreen fruit tree that belongs to the lime ( Citrus auranti
folia Swingle), Daidai ( Citrus aurantium L.),
Bergamot ( Citrus bergamia Risso et Poit.), Lemon ( Citrus limon Burm.), Natsumikan ( Citrus nat )
sudaidai Hayata), sweet orange ( Citrus sinens
is Osbeck), Navel Orange ( Citrus sinensis Osb
eck var. brasiliensis Tanaka), Cabos ( Citrus sph )
aerocarpa Hort. ex Tanaka), Unshu mikan ( Cit
rus unshiu Marcovitch) and the like are exemplified as preferable ones. Although various parts such as flowers, leaves, stems, fruits, and peels can be used, it is preferable to use leaves, fruits, or peels.

Mukuroji ( Sapindus mukurossi Gaertn.)
Is a deciduous tree belonging to the family Sapindaceae ,
Although various parts such as flowers, leaves, branch trunks, bark, and fruits can be used, it is preferable to use pericarp or bark.

Purple ( Lithospermum officinale L. v
ar. erythrorhizon Maxim.) is a member of the purple family ( Boragina
ceae ) is a perennial herb belonging to the herbal medicine "Sikon ( Lithospermi
Radix ) ”is the basic plant. Although various parts such as flowers, leaves, stems, and roots can be used, it is preferable to use roots.

The Eucalyptus ( Eucalyptus ) genus plant is first described as Eucalyptus globulus Labill.
And related plants, such as the Myrtaceae family ( My
It is an evergreen tree belonging to rtaceae ) and is the base plant of the crude drug “Eucalyptus oil” ( Oleum Eucalypti ). The closely related plants, eucalyptus Poriburakutea (Eucalypt
us polybractea RT Baker), Eucalyptus dives Schauer. Others, lemon eucalyptus ( Eucalyptus citriodora Hoo
k.), Eucalyptus piperit
a Sm.), Eucalyptus gunn
ii Hook. fil.), eucalyptus Makarutsuri (Eucal
yptus macarthuri Decne. Et Maiden), Eucalyptus rostrata Schlecht.,
Eucalyptus Sutaigeriana (Eucalyptus staiger
iana F. v. M.) and the like can also be used. Each part of leaves, branches, bark, flowers, fruits and the like and whole trees can be used, but it is preferable to use leaves.

The lily ( Lilium ) genus plants include Yamali ( Lilium auratum Lindl.) And Madonna lily ( Lilium ).
candidum L.), Lilium concolor Salis
b.), Lilium formosanum Wallace,
Sasayuri (Lilium japonicum Thunb. Ex Houtt. ), Tiger Lilies (Lilium lancifolium Thunb.), Kooniyuri (L
il ium leichtlinii Hook.f.var.maximowiczii Bake
r), lily (Liliumlongiflorum Thunb.), Iwatoyuri (Lilium maculatum Thunb.), Lilium Medeoloides (L
ilium medeoloides A. Gray), legal lily ( Lil
ium regale Wils.), Lilium rebellum Ba
ker), Lilium speciosum Thunb., and the like. These are perennials belonging to the family Liliaceae , and can be used for each part of leaves, stems, flowers, bulbs and the like, and it is preferable to use bulbs.

Examples of plants belonging to the genus Watari ( Eriophorum L.) include herring ( Eriophorum gracil Koch subsp. Corean) .
um (Palla) T. Koyama; Eriophorum coreanum Pall
a), cotton boll ( Eriophorum vaginatum L. subsp. faur
iei (EG Camus) T. Koyama; Eriophorum faurei E.
G. Camus; Scirpus faurei (EG Camu) T. Koyam
a), Takanekurosuge (Eriophorum japonicum), Ezowatasuge (Eriophorum scheuchzerii var. tenuifoliu
m ), Chihuahua sedge ( Eriophorum scheuchzerii var. s .
cheuchzerii ) and the like. These are the perennial belonging to the Cyperaceae (Cyperaceae), flowers, leaves, stems,
Various parts such as roots, spikelets, and fluff can be used.

The above herbal medicines and plant extracts include water,
Lower alcohols such as methanol, ethanol, propanol, isopropanol, 1,3-butylene glycol, propylene glycol, dipropylene glycol,
Polyhydric alcohols such as glycerin, ethers such as diethyl ether and dipropyl ether, esters such as ethyl acetate and butyl acetate, polar organic solvents such as ketones such as acetone and ethyl methyl ketone, or physiological saline and phosphoric acid It can be obtained using a buffer solution, a phosphate buffered saline, or the like. Furthermore, a method of extracting using a distillation method such as steam distillation, a method of pressing a plant to obtain an extract, and the like are exemplified, and these methods are used alone or in combination of two or more to perform extraction. The extract may be re-dissolved in water or a polar solvent as it is, or may be concentrated and dried to dryness, or may be subjected to purification treatments such as decolorization, deodorization and desalting within a range not impairing physiological effects thereof, and column chromatography. It can be used after performing a fractionation process by the like. In addition, commercially available raw materials for pharmaceuticals and cosmetics from various companies can also be used. The content of the external preparation for skin is preferably 0.0001 to 10.0% by weight, although it varies depending on the method for preparing the extract.

The hydroxy fatty acid has 2 to 3 carbon atoms.
A linear or branched saturated or unsaturated fatty acid having a hydroxyl group at the 2-position or 3-position of 0 is preferably used. For example, 2-hydroxyethanoic acid (glycolic acid), 2-hydroxypropanoic acid (lactic acid), 2-methyl-2-hydroxypropanoic acid (methyllactic acid), 2-hydroxybutanoic acid, 2-hydroxypentanoic acid, 2-hydroxy. Hexanoic acid, 2-hydroxyheptanoic acid, 2-hydroxyoctanoic acid, 2-hydroxynonanoic acid, 2-hydroxydecanoic acid, 2-hydroxyundecanoic acid, 2-
Hydroxydodecanoic acid (2-hydroxylauric acid),
2-hydroxytetradecanoic acid (2-hydroxymyristic acid), 2-hydroxyhexadecanoic acid (2-hydroxypalmitic acid), 2-hydroxyoctadecanoic acid (2-hydroxystearic acid), 2-hydroxyeicosanoic acid (2-hydroxyaraquine) Acid), 2-hydroxytetracosanoic acid (cerebronic acid), 2-hydroxy-
9-undecenoic acid (2-hydroxy-9-undecylenic acid), 2-hydroxy-10-undecenoic acid (2-hydroxy-10-undecylenic acid), 2-hydroxy-c
is-15-tetracosenoic acid (2-hydroxynervonic acid), lanolin-derived long-chain 2-hydroxy fatty acid, 3-
Hydroxypropanoic acid, 2-methyl-3-hydroxypropanoic acid, 3-hydroxybutanoic acid, 3-hydroxypentanoic acid, 3-hydroxyhexanoic acid, 3-hydroxyheptanoic acid, 3-hydroxyoctanoic acid, 3-hydroxynonanoic acid, 3-hydroxydecanoic acid, 3-hydroxyundecanoic acid, 3-hydroxydodecanoic acid (3-hydroxylauric acid), 3-hydroxytetradecanoic acid (3
-Hydroxymyristic acid), 3-hydroxyhexadecanoic acid (3-hydroxypalmitic acid), 3-hydroxyoctadecanoic acid (3-hydroxystearic acid), 3
-Hydroxyeicosanoic acid (3-hydroxyarachidic acid), 3-hydroxytetracosanoic acid, 3-hydroxy-9-undecenoic acid (3-hydroxy-9-undecylenic acid), 3-hydroxy-10-undecenoic acid (3- Hydroxy-10-undecylenic acid), and 3-hydroxy-cis-15-tetracosenoic acid (3-hydroxynervonic acid), 2,3-dihydroxypropanoic acid (glyceric acid), erythronic acid, threonic acid, and the like 2,3, 2,3,4,5-tetrahydroxypentanoic acid, such as 4-trihydroxybutanoic acid, ribbon acid, arabinonic acid, xylonic acid, lyxonic acid, alloic acid, altronic acid, gluconic acid,
2,3,4,5,6-pentahydroxyhexanoic acid such as mannonic acid, gulonic acid, idonic acid, galactonic acid and talonic acid, 2,3 such as glucoheptonic acid and galactoheptonic acid,
4,5,6,7-Hexahydroxyheptanoic acid, hydroxymethanedicarboxylic acid (tartronic acid), 1-hydroxyethane-1,2-dicarboxylic acid (malic acid), 1,2-
1,2, such as dihydroxyethane-1,2-dicarboxylic acid (tartaric acid), 2-hydroxypropane-1,2,3-tricarboxylic acid (citric acid), glucaric acid and mutic acid
3,4-tetrahydroxybutane-1,4-dicarboxylic acid and the like can be mentioned. These hydroxy fatty acid salts include alkali metal salts such as sodium salts and potassium salts, alkaline earth metal salts such as magnesium salts and calcium salts,
Examples thereof include alkanolamine salts such as ammonium salts and triethanolamine salts, and basic amino acid salts such as D-, L- or DL-arginine, lysine and histidine.
Examples of these derivatives include sterol esters such as alkyl or alkenyl esters, phosphoric acid esters, sulfuric acid esters, phosphatidyl esters, cholesteryl esters, sitosteryl esters, stigmasteryl esters, and amides. In the present invention, one or more selected from these are used. The amount of the total amount of the external preparation for skin is not particularly limited,
It is suitable to set it to about 0.0001 to 10.0% by weight.

Nucleic acid and its related substances include deoxyribonucleic acid and ribonucleic acid obtained by extraction from various tissues of animals and plants, yeast, bacteria and the like, ribonucleotides such as adenosine, guanosine, inosine, xanthosine, cytidine, uridine and ribothymidine. Deoxyribonucleosides such as nucleosides, deoxyadenosine, deoxyguanosine, deoxyinosine, deoxyxanthosine, deoxycytidine, deoxyuridine, thymidine, adenosine monophosphate, adenosine diphosphate, adenosine triphosphate, guanosine monophosphate, guanosine diphosphate Acid, guanosine triphosphate, inosine monophosphate, inosine diphosphate, inosine triphosphate, xanthosine monophosphate, xanthosine diphosphate, xanthosine triphosphate, cytidine monophosphate, cytidine diphosphate, cytidine triphosphate Ribonucleotides such as acid, uridine monophosphate, uridine diphosphate, ribothymidine monophosphate, ribothymidine diphosphate, ribothymidine triphosphate, deoxyadenosine monophosphate, deoxyadenosine diphosphate, deoxyadenosine triphosphate, deoxy Guanosine monophosphate, deoxyguanosine diphosphate, deoxyguanosine triphosphate, deoxyinosine monophosphate, deoxyinosine diphosphate, deoxyinosine triphosphate, deoxyxanthosine diphosphate, deoxyxanthosine diphosphate, deoxy Xanthosine triphosphate, deoxycytidine monophosphate, deoxycytidine diphosphate, deoxycytidine triphosphate, deoxyuridine monophosphate, deoxyuridine diphosphate, thymidine monophosphate, thymidine diphosphate,
Deoxyribonucleotides such as thymidine triphosphate, cyclic adenosine monophosphate, cyclic guanosine monophosphate, cyclic cytidine monophosphate, cyclic uridine monophosphate and other cyclic nucleotides, coenzyme A, nicotinamide adenine diphosphate Examples thereof include nucleotides, nicotinamide adenine dinucleotide phosphate, coenzymes such as flavin adenine dinucleotide, and salts thereof, and one or more of them are selected and used. The blending amount per total amount of the skin external preparation is not particularly limited, but it is suitable to be about 0.0001 to 10.0% by weight.

As the protein extracted from the eggshell membrane,
As the eggshell membrane, a membrane attached to the inside of the eggshell of birds such as chicken eggs and quail is used, and extracted from these with one or more of water and an organic solvent, or solubilization or treatment with acid, alkali or enzyme treatment. What is obtained by hydrolysis is used. A commercially available product may be used as a raw material for cosmetics. The blending amount per total amount of the skin external preparation is not particularly limited, but it is suitable to be about 0.0001 to 10.0% by weight.

As sericin and its hydrolyzate, those obtained by partially hydrolyzing sericin contained in silkworm cocoons or raw silk in an alkaline aqueous solution or by enzymatic treatment to be eluted can be used. The eluate is adjusted to pH 3 to 5 with an organic acid or an inorganic acid, or mixed with a water-soluble organic solvent such as methanol, ethanol or dioxane to precipitate a sericin hydrolyzate, which is filtered and dried to obtain a powder. It can also be used. Furthermore, sericin and its hydrolyzate used in the present invention have an average molecular weight of about 10,000.
What is -20,000, and what contains 20-40 mol% of serine is especially preferable. The blending amount per total amount of the skin external preparation is not particularly limited, but it is suitable to be about 0.0001 to 10.0% by weight.

The lactic acid bacterium extract and the bifidobacteria extract used in the present invention are not particularly limited as long as they can be used in ordinary skin external preparations. The total amount of the external preparation for skin is not particularly limited, but is 0.00
It is suitable to set the content to about 01 to 10.0% by weight.

The algae extract used in the present invention is not particularly limited as long as it is an algae extract used in ordinary skin external preparations. The algae for obtaining an extract, green algae, brown algae, alone one selected from red algae, or two or more are used, Yahazugusa genus among them (Di
ctyopteris ), Genus Honda Walla ( Sargassum ), Genus Fukulinami ( Dilophus ), Genus Fukuronori ( Colpomenia ),
Laminaria , Ceratodicty
on ), Rhododendron ( Asparagopsis ), Rhododendron ( Po
lysiphonia ), Salix genus ( Chondria ), Sozo ( La )
urencia ), Rattle ( Galaxaura ), Quitoceros
( Chaetoceros ), it is preferable to use an extract of one or more algae selected from the genus Pavlova .

Algae of the genus Dictyopteris are
Brown alga A kind of alga in the family Astragaliaceae.
The algae of the genus Narcissus include Dictyopter
is latiuscula ), Dwarf seaweed ( Dictyopteris undulat
a ), Herayahazu ( Dictyopteris prolifera ), Suzyahazu ( Dictyopteris plagiogramma ), Himeyahazu ( Dic
tyopteris repens ), Ezohahaz ( Dictyopteris divar )
icata ), Uraboshijahazu ( Dictyopteris polypodioide
s ) etc. are illustrated. Among these algae of the genus Astragalus, from the viewpoint of the dermal fibroblast activating action, an extract of Ulaboshihahazu ( Dictyopteris polypodioides ) is preferably used.

The algae of the genus Sargassum include Sargassum fulvellum , Sargassum yendoi , and Sargassum pilu.
riferum ), Yatumatamoku ( Sargassum patens ), Akamoku ( Sargassum horneri ), Sawmillimoku ( Sargassum s )
erratifolium ), Scutellaria baicalensis ( Sargassum giga)
nteifolium), Yoremoku (Sargassum tortile), Yanagimoku (Oobamoku: Sargassum ringgoldianum), Nejimoku (Sargassum sagamianum), Hahakimoku (Sargas
sum kjellmanianum ), sea turtle ( Sargassum thunb )
ergii ), Fusijimoku ( Sargassum confusum ), Isomok ( Sargassum hemiphyllum ), Narasamo ( Sargassum )
nigrifolium), Togemoku (Sargassum micracanthu
m ), Tamanashimoku ( Sargassum nipponicum ), Jin Meso ( Sargassum vulgare ), Futaemoku (Hiragimoku: Sargassum duplicatum ), Ezononemoku ( Sargas )
sum yezoense ) etc. are illustrated. Among these algae of the genus Astragalus, in terms of the dermal fibroblast activating action, pea ( Sargassum yendoi ) and ezononejimoku ( Sargas )
sum yezoense ), and Yasumatamoku ( Sargassum paten)
It is preferable to use one or more extracts selected from s ).

The algae of the genus Fuculina ( Dilophus ) are
Brown alga A kind of alga in the family Astragaliaceae.
The algae of the genus Fuchlinamidii include Fuchlinamidii ( Di
lophus okamurai , Dilophus marginata ) are exemplified.

The algae of the genus Colpomenia is one of the algae of the brown alga Cyamonoliaceae. The algae of the genus Fukuronori include the Fukuronori ( Colpomenia s
inuosa ) and cottonweed ( Colpomenia bullosa ). Among these algae, it is preferable to use the extract of Fukuronori ( Colpomenia sinuosa) from the viewpoint of dermal fibroblast activation activity.

The algae of the genus Laminaria is one of the algae of the family Astragalus of the brown seaweed order. The algae Laminaria spp, Laminaria japonica (Laminaria japonica), Hosomekonbu (Laminaria religiosa), Mitsuishikonbu (Lamin
aria angusta ), Japanese kelp ( Laminaria cichorioid )
es ), Himekonbu ( Laminaria longipes ) and the like. Among these kelp algae, from the point of dermal fibroblast activating action, Laminaria religios ( Laminaria religios)
Preference is given to using the extract of a ).

The algae of the genus Ceratodictyon is one of the algae of the red alga Cyperaceae, the family Lagomorpha ,
An example is Kamensou ( Ceratodictyon spongiosum ).

Algae of the genus Asparagopsis are
Red alga A kind of algae of the family Kaedekenori,
Kagikenori ( Asparagopsis taxiformis ), Kaginori ( A
sparagopsis hamifera ) etc. are illustrated. Among these, from the viewpoint of dermal fibroblasts activation action, Kagikenori (As
It is preferable to use an extract of Paragopsis taxiformis ).

The algae of the genus Polysiphonia is one of the algae of the red alga, Cucurbitaceae, Fujimatsumoaceae . The algae of the genus Astragalus are Polysiphonia mor.
rowii ), Dwarf Kennori ( Polysiphonia urceolat
a ), Yellow sedge ( Polysiphonia senticulosa ), Yellow squirrel ( Polysiphonia japonica ), Black sedge ( Polysiphonia forcipata ), Red sedge ( Polysi )
phonia crassa ) etc. are illustrated. Of these algae of the genus Astragalus, it is preferable to use the extract of Polysiphonia morrowii from the viewpoint of the dermal fibroblast activation activity.

The algae of the genus Chondria is one of the algae of the red alga, Anthracene, Fujimatsumoaceae. The algae of the genus Willowaceae include the Yuna ( Chondria crassicauli).
s ), willow nori ( Chondria dasyphylla ), akayuna ( Chondria atropurpurea ), mothleuna ( Chondria in
tricata ), hanayanagi ( Chondria armata ), crocodile ( Chondria ryukyuensis ), and the like. Among these algae of the genus Willowaceae, it is preferable to use the extract of Yuna ( Chondria crassicaulis ) from the viewpoint of dermal fibroblast activation.

The algae of the genus Laurencia are one of the algae of the red alga, Aceraceae, Fujimatsumoaceae. The algae of the genus Sozo include Sozo sp. ( Laurencia sp.) And Kurosozo ( Laur
encia intermedia ), Mitsu de Sozo ( Laurencia okamura
i ), Sozonohana ( Laurenciagrevilleana ), Oosozo ( Laurencia glandulifera ), Honeysoso ( Laurencia pi )
nnata ), Cobusoso ( Laurencia undulata ) and the like. Among these algae of the genus Sozo, it is preferable to use an extract of Cobusozo ( Laurencia undulata ) from the viewpoint of dermal fibroblast activation action.

The algae of the genus Galaxaura is one of the algae of the red alga Rhinoceros Ornithidae rattle family. The rattle algae are Galaxaura fastigiat.
a), Hiragaragara (Galaxaura falcata), beads rattle (Galaxaura obtusata) and the like. Among these algae, it is preferable to use an extract of the rattle rattle ( Galaxaura falcata ) from the viewpoint of dermal fibroblast activation activity.

The genus Chaetoceros and the genus Pavlova belong to Chaetoceracea.
e ) It is a kind of diatom belonging to the family, and it is particularly preferable to use Chaetoceros gracilis .

The site from which the extract is collected from algae is not particularly limited, but it is preferably extracted from whole algae or mekabu (spore leaves or adult leaves). In addition, algae are used as raw or dried. The extraction solvent is not particularly limited, and monohydric alcohols such as water, ethanol, methanol, isopropanol, isobutanol, n-hexanol, methylamyl alcohol, 2-ethylbutanol, n-octyl alcohol, glycerin, ethylene glycol, ethylene. Glycol monomethyl ether,
Propylene glycol, propylene glycol monomethyl ether, propylene glycol monoethyl ether, triethylene glycol, 1,3-butylene glycol, hexylene glycol and other polyhydric alcohols or derivatives thereof, acetone, methyl ethyl ketone, methyl isobutyl ketone, methyl-n-propyl Examples thereof include ketones such as ketones, esters such as ethyl acetate and isopropyl acetate, ethers such as diethyl ether and diisopropyl ether. Further, a polar solvent added with an inorganic salt such as phosphate buffered saline or a solvent added with a surfactant can be used, and there is no particular limitation. The amount of such algae extract blended per total amount of the skin external preparation is not particularly limited, but it is suitable to be about 0.0001 to 10.0% by weight.

Nigari is a residual salt remaining after concentrating seawater and precipitating sodium chloride. In addition to sodium chloride, potassium chloride, magnesium sulfate, magnesium bromide, magnesium chloride, sodium sulfate, potassium bromide, calcium chloride, etc. Is a mixture of. The bittern may be added either in a state of being dissolved in the aqueous phase component or in a crystalline state such as granular form. The blending amount per total amount of the skin external preparation is not particularly limited, but it is suitable to be about 0.0001 to 10.0% by weight.

Examples of vitamins include retinol, vitamin A oil, retinol acetate, and other vitamin A, riboflavin, riboflavin butyrate, and riboflavin, and other vitamin B ₂ ; pyridoxine hydrochloride, and other vitamin B ₆ ; nicotinic acid, nicotinic acid amide, and nicotine. Nicotinic acid and its salts and derivatives such as benzyl acidate, vitamin Ds such as ergocalciferol and cholecalciferol, and vitamin H such as biotin
And the like. The blending amount per total amount of the skin external preparation is not particularly limited, but it is suitable to be about 0.0001 to 10.0% by weight.

As the spleen extract, a spleen of a fresh mammal extracted with water and deproteinized is used. The amount of the total amount of the external preparation for skin is not particularly limited,
It is suitable to set it to about 0.0001 to 1.0% by weight.

Fibroblast growth factor (FGF) and epidermal growth factor (EGF) are proteins that bind to tyrosine kinase type receptors and are involved in dermal fibroblast and epidermal cell growth promotion, respectively. These are those isolated from humans or mammals. The blending amount per total amount of the skin external preparation is not particularly limited, but 0.0001 to
Appropriately about 1.0% by weight.

The external preparation for skin according to the present invention can be provided in various dosage forms such as lotions, emulsions, gels, creams, ointments, powders and granules. In addition, skin cosmetics such as lotion, emulsion, cream, beauty essence, pack,
Makeup base lotion, base cosmetics such as makeup base cream, foundation of each dosage form such as milky liquid, oily, solid, etc., makeup cosmetics such as eye color, cheek color, cleansing cream, cleansing lotion, cleansing foam , It can also be provided as a skin cleansing agent such as face wash soap and body shampoo, and a hair cosmetic such as hair shampoo, hair rinse and hair treatment.

In addition to the Salixacea plant extract and cell activator, the external preparation for skin according to the present invention includes oily ingredients, surfactants, humectants, pigments, ultraviolet absorbers, and antioxidants. A general raw material for medicines and cosmetics such as fragrances and antifungal agents, and physiologically active ingredients such as anti-inflammatory agents and whitening agents can be contained.

[0091]

EXAMPLES The features of the present invention will be described in detail with reference to Examples.

First, preparation of a Salicaceae plant extract contained in the external preparation for skin according to the present invention will be described.

[Crown willow bark extract] To 200 g of fresh bark of Salix nigra collected in the spring was shredded, 1000 g of purified water was added, and the mixture was extracted at 25 ° C for 36 hours with stirring. The obtained extract was filtered and then concentrated under reduced pressure to obtain 150 g of willow bark extract.

[European porcupine leaf extract] 200 g of fresh leaves of European porcupine ( Populus tremula )
1000g of purified water is added to the shredded product,
Extract for 36 hours with stirring. The obtained extract was filtered and then concentrated under reduced pressure to obtain 120 g of a porcupine leaf extract.

[European willow bark extract] Dried European willow ( Salix alba L .; Salix aurea Salis
b.) 50g is crushed and 1000g of 50% by volume ethanol
And extract at room temperature for 24 hours. The obtained extract was filtered, concentrated under reduced pressure, and then freeze-dried to obtain 5 g of a willow bark extract.

[Weeping willow leaf extract] 10 g of fresh weeping willow ( Salix babylonica L.) sprouts
Purified water (100 g) is added to and the mixture is stirred and extracted with a mixer for 10 minutes. The obtained extract was filtered, concentrated under reduced pressure, and lyophilized to obtain 1.2 g of weeping willow leaf extract.

Next, the cell activating agent contained in the external preparation for skin according to the present invention will be described.

[Yeast extract] Saccharomyces cerevisiae Meyer was cultivated, and 500 g of cells were autolysed to obtain a lysate, which was filtered, concentrated and freeze-dried to obtain a yeast extract.

[Yeast culture supernatant] Saccharomyces cerevisiae Meyer was cultured, and the culture supernatant in a steady state was aseptically filtered with a membrane filter, and the filtrate was used as the yeast culture supernatant.

[Kakkon Extract] Dry powder of Kakkon 2
Soak 00g in 1 liter of ethanol and run at 20 ° C for 7
The mixture was allowed to stand for extraction for a day, and the extract was filtered, collected, concentrated, dried, and freeze-dried to give the titled Kacon extract.

[Each extract of Chamomile, Kidachi aloe, Mannenrou] Head flower of Camellia ( Matricaria chamomilla L.), Aloe arborescens Mill. Var.
natalensis Berg.) leaf, mannen wax ( Rosmarinus
officinalis L.) leaves of 500 g each are dried and crushed, immersed in 1 liter of 50% by volume ethanol aqueous solution, allowed to stand at 20 ° C. for 7 days, then filtered to collect the filtrate, and extract each of the above-mentioned plants. It was a thing.

[ Ginkgo biloba] Ginkgo bil
(Oba L.) leaves (600 g) were dried, pulverized, immersed in 1 liter of 1,3-butylene glycol, and extracted by stirring at 25 ° C. for 3 days. The extract was filtered to collect the filtrate, which was used as the title plant extract.

[ Equisetum arve ] Equisetum arve
600 g of whole plant ( nse L.) was finely chopped , dipped in 1 liter of 50% by volume aqueous glycerin solution, and extracted by stirring at 20 ° C. for 5 days. The extract was filtered to collect the filtrate, which was used as the title plant extract.

[Safflower extract] safflower ( Carhamus
tinctorius L.) 1 kg of flowers are squeezed into a plate,
Dip in 1.5 liters of ethanol and stir at 15 ℃
It was left standing for a day for extraction. The extract was filtered to collect the filtrate, which was used as the title plant extract.

[Ginseng extract] 1 kg of dried root of Panax ginseng CA Meyer was added to 5
Immerse in 1.5 liters of 0% by weight ethanol, 15 ℃
The sample was allowed to stand for 10 days for extraction. The extract was filtered to collect the filtrate, which was used as the title plant extract.

[Beech extract] “GA
A commercially available raw material provided under the trade name of "TULINE RC" was used. This product is beech ( Fagus crenata Blume)
It is prepared by extracting with water from the young buds.

[Eggshell Membrane Extracted Protein] An egg shell membrane hydrolyzate commercially available under the trade name “EM Protein-L” from QP Corporation was used.

[Deoxyribonucleic acid potassium salt] Commercially available organic synthetic chemicals, trade name "DNA-K" was used.

[Algae extract] 1,3-butylene glycol 50 of the genus Astragalus spp. Sold under the trade name of "Algerex S" by Ichimaru Falcos Co., Ltd. A wt% aqueous extract was used.

[Ceramide] The ceramide derived from rice bran, which is sold by Oryza Yuka Co., Ltd. under the trade name of “Oriza ceramide”, was used.

Next, the formulations of the examples of the external preparation for skin according to the present invention will be shown.

[Examples 1 to 15 and Comparative Examples 1 to 5] Emulsion (1) Cetanol 1.00 (% by weight) (2) Beeswax 0.50 (3) Vaseline 2.00 (4) Squalane 6. 00 (5) Dimethyl polysiloxane 2.00 (6) Polyoxyethylene (20EO) sorbitan monostearate 1.00 (7) Glycerin monostearate 1.00 (8) Glycerin 4.00 (9) 1, 3-Butylene glycol 4.00 (10) Methyl paraoxybenzoate 0.10 (11) Salicaceae plant extract shown in Table 1 1.00 (12) Purified water Amount based on 100 (13) Carboxy Vinyl polymer (1.0% aqueous solution) 10.00 (14) Potassium hydroxide (10.0% aqueous solution) 1.00 (15) Cell activating agent shown in Table 1 Amount shown in Table 1 Production method: (1) to ( Mix the oil phase components of 7), heat and dissolve to 75 ℃. On the other hand, the water phase components (8) to (12) are mixed and dissolved to 75 ° C. After preliminarily emulsifying by adding the oil phase to this, (13) is added to uniformly emulsify with a homomixer, then (14) is added to thicken and then cooled,
Add (15) at 40 ° C. and mix.

[0113]

[Table 1]

The usage test was conducted on Examples 1 to 15 and Comparative Examples 1 to 5 of the present invention, and the moisture retention and
The effects of improving skin aging symptoms and skin roughness were evaluated.

First, regarding the moisturizing property, for each sample,
Twenty female panelists in their 20s to 50s were used in the blind for 2 weeks to evaluate the moist feeling. The evaluation was carried out in five grades of "very moist", "present", "somewhat", "somewhat", and "not".
The results are shown in Table 2 by the number of panelists given each evaluation.

[0116]

[Table 2]

As is clear from Table 2, Salixae ( Sali
caceae ) A plant extract and a cell activator were used in combination in each example. In the use groups, a very high evaluation was obtained regarding moisturizing ability , and a comparative example in which a Salixaceae plant extract was added alone 2 to Comparative Example 5 Good results were obtained as compared with the use group.

Next, for the improvement of skin aging symptoms, for each sample, 20 male and female panelists in their 50s to 60s who had such symptoms were used blindly for 1 month. The change in state was observed and evaluated. Skin wrinkles, texture,
The three items of the acupuncture were selected, and wrinkles and texture were observed by taking photographs and replicas of the skin surface, and the improvement status was evaluated. The skin elasticity was evaluated by measuring the skin elasticity. The improvement status was evaluated in three stages of “improvement”, “slight improvement” and “no change”, and Table 3 shows the number of panelists who obtained each evaluation.

[0119]

[Table 3]

As is clear from Table 3, wrinkles and skin elasticity were tended to be improved to some extent in each of the groups used in Comparative Examples which did not contain a cell activating agent, but Salixacea plant extract In the group used in Examples in which both and the cell activator were used in combination, more panelists recognized a clear improvement than in the corresponding groups used in Comparative Examples.

For the improvement of rough skin, 20 samples of female panelists each having rough skin were used blindly for 1 month for each sample, and the improvement of rough skin was evaluated. The evaluation was made in five stages: "improved", "slightly improved", "no change", "slightly worse", and "worse". The results are shown in Table 4 by the number of panelists given each evaluation.

[0122]

[Table 4]

As is clear from Table 4, Salixae ( Sali
caceae ) In the use groups of the examples of the present invention in which the plant extract and the cell activator were used in combination, remarkable improvement in rough skin was observed in all cases, and the skin condition was almost good after the end of the use test. Had been improved up to. On the other hand, in the comparative example use group, although there was a case in which the skin roughness was considerably improved, the degree thereof was smaller than that of the corresponding example use group.

As described above, in the external preparation for skin of the example of the present invention, as compared with the conventional comparative example, the excellent moisturizing effect,
It had the effect of improving skin aging and rough skin.

Example 16 Lotion (1) Ethanol 20.0 (wt%) (2) Polyoxyethylene (60EO) hydrogenated castor oil 1.0 (3) Methyl paraoxybenzoate 0.1 (4) Europe Porcupine leaf extract 1.0 (5) 2-hydroxyacetic acid 0.5 (6) dipropylene glycol 5.0 (7) 1,3-butylene glycol 10.0 (8) purified water 62.4 Production method: (1 (2) and (3) are added to () and dissolved to form an alcohol phase. On the other hand, (4) to (7) are sequentially dissolved in (8) to form an aqueous phase. The alcohol phase is added to the aqueous phase, and the mixture is stirred and mixed.

Example 17 Emulsion (1) Cetanol 1.0 (wt%) (2) Beeswax 0.5 (3) Vaseline 2.0 (4) Squalane 6.0 (5) Dimethylpolysiloxane 2.0 (6) Polyoxyethylene (20EO) sorbitan 1.0 Monostearic acid ester (7) Glyceryl monostearic acid ester 1.0 (8) Glycerin 4.0 (9) 1,3-Butylene glycol 4.0 (10) Methyl paraoxybenzoate 0.1 (11) Willow bark extract 0.5 (12) 2-hydroxyacetic acid 0.2 (13) Purified water 61.7 (14) Carboxyvinyl polymer 10.0 (1.0 weight) % Aqueous solution) (15) Potassium hydroxide (10.0 wt% aqueous solution) 1.0 (16) Ethanol 5.0 Manufacturing method: The oil phase components of (1) to (7) are mixed and heated to dissolve 75
℃. On the other hand, the water phase components (8) to (13) are mixed and dissolved to reach 75 ° C. After adding the oil phase to this and pre-emulsifying, add (14) and homogenize with a homomixer, then add (15) to thicken and cool, and cool at 40 ° C (16). Add and mix.

[Example 18] Oil-in-water cream (1) Beeswax 6.00 (% by weight) (2) Cetanol 5.00 (3) Reduced lanolin 8.00 (4) Squalane 27.50 (5) Glycyrrhetin Acid stearyl 0.05 (6) Glyceryl fatty acid ester 4.00 (7) Lipophilic glyceryl monostearate 2.00 (8) Polyoxyethylene (20EO) sorbitan 5.00 Monolaurate (9) Stearyl glycyrrhetinate 0.25 (10) Retinol 0.20 (11) Propylene glycol 5.00 (12) Methyl paraoxybenzoate 0.10 (13) Weeping willow leaf extract 1.00 (14) Purified water 35.90 Manufacturing method: (1 )-(10) Oil phase components are mixed and melted to 75 ° C. On the other hand, (11) to (14) are mixed, dissolved and heated to 75 ° C. Next, the oil phase component is added to this aqueous phase component to pre-emulsify it, then homogenize with a homomixer and cool.

[Example 19] Oil-in-water cream (1) Beeswax 6.0 (wt%) (2) Cetanol 5.0 (3) Reduced lanolin 8.0 (4) Squalane 27.5 (5) Glyceryl Fatty acid ester 4.0 (6) Lipophilic glyceryl monostearate 2.0 (7) Polyoxyethylene (20EO) sorbitan 5.0 Monolaurate (8) Propylene glycol 5.0 (9) Methyl paraoxybenzoate 0.1 (10) Willow bark extract 0.3 (11) Purified water 37.0 (12) Kujin extract 0.1 Process: Mix and dissolve the oil phase components of (1) to (7) to 75 ℃. On the other hand, (9) and (10) are dissolved in (8), added to (11), mixed and dissolved, and heated to 75 ° C. Next, the oil phase component is added to the aqueous phase component to pre-emulsify it, then homogenize with a homomixer, and after cooling, (12) is added and mixed at 40 ° C.

Example 20 Gel Agent (1) Dipropylene Glycol 10.0 (wt%) (2) Carboxyvinyl Polymer 0.5 (3) Potassium Hydroxide (10.0 wt% Aqueous Solution) 1.0 ( 4) Methyl paraoxybenzoate 0.1 (5) European porcupine leaf extract 0.2 (6) Peony extract 0.3 (7) Purified water 87.9 Production method: (7) to (1) to (6). Are sequentially added to dissolve uniformly.

[Example 21] Oil-in-water emulsion type ointment (1) White petrolatum 25.0 (% by weight) (2) Stearyl alcohol 25.0 (3) Glycerin 12.0 (4) Sodium lauryl sulfate 1.0 (5) Methyl paraoxybenzoate 0.1 (6) Purified water 35.4 (7) Willow bark extract 0.5 (8) Hydrolyzed sericin aqueous solution 1.0 Process: Oils of (1) to (4) The phase components are mixed and heated to dissolve uniformly and reach 75 ° C. On the other hand, mix the water phase components (5) to (7),
Heat to 75 ° C. The oil phase component was gradually added to this water phase component while stirring to emulsify and cool the mixture.
Add (8) at ℃ and mix.

[Example 22] Liposome agent (1) Glycerin 2.0 (% by weight) (2) 1,3-butylene glycol 3.0 (3) Polyoxyethylene (25EO) oleyl ether 0.2 (4) Ethanol 10.0 (5) Methyl paraoxybenzoate 0.1 (6) Purified water 79.7 (7) Willow bark extract 5.0 Kacon extract-encapsulating liposome Manufacturing method: Dissolve (5) in (4), Add (1) to (3) to (6) and mix evenly, and add (7) to this and disperse. The (7) black willow bark extract and licorice extract-encapsulated liposomes are 50% by volume containing 1.0 (w / v)% of willow bark extract and 2.0 (w / v)% of cuckoo extract.
80 g of soybean lecithin was added to 100 mL of an aqueous ethanol solution, suspended at 55 ° C., and then sonicated to prepare liposomes, which were recovered by centrifugation.

[Example 23] Water-in-oil emollient cream (1) Liquid paraffin 30.0 (% by weight) (2) Microcrystalline wax 2.0 (3) Vaseline 5.0 (4) Diglyceryl dioleic acid Ester 5.0 (5) L-Glutamate sodium 1.6 (6) L-serine 0.4 (7) Propylene glycol 3.0 (8) Methyl paraoxybenzoate 0.1 (9) Weeping willow leaf extract 0. 5 (10) Bittern 1.5 (11) Purified water 50.8 (12) Perfume 0.1 Manufacturing method: (5) and (6) are dissolved in a part of (11) to 50 ℃, 50 ℃ beforehand Add slowly to the warmed (4) with stirring. These are mixed in advance and uniformly dispersed in (1) to (3) which are dissolved by heating at 70 ° C. Add (7) to (10) to (1
Add to the rest of 1), heat to 70 ° C, add with stirring, and emulsify with a homomixer. After cooling, (12) is added and mixed at 40 ° C.

[Example 24] Makeup base cream (1) Stearic acid 12.00 (% by weight) (2) Cetanol 2.00 (3) Glyceryl tri-2-ethylhexanoate 2.50 (4) Self-emulsification Glyceryl monostearic acid 2.00 Ester (5) Propylene glycol 10.00 (6) European porcupine leaf extract 0.02 (7) Methyl paraoxybenzoate 0.10 (8) Lactic acid bacterium extract 0.05 (9) Potassium hydroxide 0.30 (10) Purified water 68.43 (11) Titanium oxide 2.00 (12) Red iron oxide 0.40 (13) Yellow iron oxide 0.10 (14) Perfume 0.10 Manufacturing method: (1) The oil phase components (4) to (4) are mixed and dissolved to reach 75 ° C. On the other hand, the components (5) to (10) are mixed, heated and dissolved, and the pigment components (11) to (13) are added thereto, and the components are uniformly dispersed with a homomixer to 75 ° C. Then, the oil phase component is added to the aqueous phase component to uniformly emulsify with a homomixer,
After cooling, (14) is added and mixed at 40 ° C.

Example 25 Emulsion foundation (1) Stearic acid 2.00 (wt%) (2) Squalane 5.00 (3) Octyldodecyl myristate 5.00 (4) Cetanol 1.00 (5) Decaglyceryl monoisopalmitic acid ester 9.00 (6) 1,3-butyleneglycol 6.00 (7) Methyl paraoxybenzoate 0.10 (8) Potassium hydroxide 0.08 (9) Purified water 52.52 (10) Titanium oxide 9.00 (11) Talc 7.40 (12) Red iron oxide 0.50 (13) Yellow iron oxide 1.10 (14) Black iron oxide 0.10 (15) European willow bark extract 1. 00 (16) Kacon extract 0.05 (17) Perfume 0.15 Production method: The oil phase components (1) to (5) are mixed and dissolved to 75 ° C. On the other hand, the water phase components (6) to (9) are mixed, heated and dissolved, and the pigment components (10) to (14) are added thereto, and the mixture is uniformly dispersed with a homomixer to 75 ° C. Next, the oil phase component is added to the aqueous phase component, and the mixture is uniformly emulsified with a homomixer. After cooling, (15) to (17) are added and mixed at 40 ° C.

Example 26 Hand Cream (1) Cetanol 4.00 (wt%) (2) Vaseline 2.00 (3) Liquid Paraffin 10.00 (4) Glyceryl Monostearate 1.50 (5) Polyoxyethylene (60EO) glyceryl 2.50 isostearic acid ester (6) tocopherol acetate 0.25 (7) glycerin 20.00 (8) methyl paraoxybenzoate 0.10 (9) weeping willow leaf extract 0.50 (10 ) DNA-K 0.02 (11) Purified water 59.13 Manufacturing method: Mix and dissolve the oil phase components (1) to (6) to 75 ° C. On the other hand, (8) and (9) are dissolved in (7) and (10) together with (11)
In addition, it mixes and melt | dissolves and makes it 75 degreeC. Next, the oil phase component is added to the aqueous phase component, and the mixture is uniformly emulsified with a homomixer and cooled.

[Example 27] Pack (1) Purified water 69.0 (% by weight) (2) Polyvinyl alcohol 12.5 (3) Ethanol 10.0 (4) Glycerin 5.0 (5) Polyethylene glycol (average) Molecular weight 1540) 3.0 (6) Black willow bark extract 0.3 (7) Yeast culture supernatant 0.2 Production method: The components (2) to (7) are sequentially added to (1),
Mix, dissolve and homogenize.

[0137]   Example 28 Hair artic (1) Ethanol 50.0 (wt%) (2) Purified water 48.4 (3) Sodium citrate 0.1 (4) European porcupine leaf extract 1.5 Manufacturing method: Components (1) to (4) are mixed and uniformly dissolved.

[Example 29] Hair shampoo (1) Sodium alkyl ether sulfate 18.0 (% by weight) (2) Coconut oil fatty acid diethanolamide 2.0 (3) Propylene glycol 2.0 (4) European willow bark extraction Product 0.5 (5) Yeast extract 0.1 (6) Purified water 77.4 Production method: The components (1) to (5) are sequentially added to (6),
Make it uniform.

Example 30 Hair rinse (1) Cetanol 3.0 (wt%) (2) Stearyl trimethyl ammonium chloride 0.7 (3) Glycerin 3.0 (4) N-cocoyl-L-arginine ethyl ester -DL-pyrrolidone carboxylate 0.1 (5) Bifidobacteria extract 0.1 (6) Weeping willow leaf extract 3.0 (7) Purified water 90.1 Production method: (1)-(6) components Add to (7) sequentially,
Mix.

[Example 31] Liquid body shampoo (1) N-lauroyl-L-glutamic acid 20.0 (wt%) triethanolamine (30.0 wt% aqueous solution) (2) N-lauroylmethyltaurine sodium 10. 0 (30.0 wt% aqueous solution) (3) triethanolamine laurate 10.0 (4) triethanolamine myristate 10.0 (5) lauroyl imidazolinium betaine 5.0 (6) lauroyl diethanolamide 5. 0 (7) Propylene glycol 7.0 (8) Black willow bark extract 1.0 (9) Chamomile extract 0.5 (10) Purified water 31.5 Process: The components (1) to (9) are sequentially added. Add to (10) and mix.

Example 32 Face Wash Foam (1) Myristic Acid 18.0 (wt%) (2) Palmitic Acid 3.0 (3) Stearic Acid 7.0 (4) Mixed Fatty Acid Triglyceride 0.1 (5) Stearyl glycyrrhetinate 0.1 (6) Self-emulsifying glyceryl monostearate 3.0 (7) Purified water 37.3 (8) Glycerin 17.0 (9) Potassium hydroxide 7.8 (10) Diglycerin 3. 0 (11) 1,3-butylene glycol 1.0 (12) N-stearolyl-L-glutamate disodium 1.0 (13) Methyl paraoxybenzoate 0.1 (14) Black willow bark extract 1.0 (15 ) European porcupine leaf extract 0.6 Manufacturing method: The oil phase components of (1) to (6) are mixed and dissolved by heating to 70 ° C. The aqueous phase components (7) to (15) are mixed, dissolved and heated to 70 ° C. The above oil phase is gradually added to this aqueous phase component for saponification, and then cooled while mixing.

Regarding Examples 1 to 32, 2
No change in condition was observed when stored at 5 ° C for 6 months, and no problem skin irritation reaction was observed in a 48-hour back occlusion patch test conducted by 30 male panelists.

[0143]

As described in detail above, according to the present invention, it is possible to obtain a skin external preparation having an excellent moisturizing effect and synergistically improving the skin aging symptoms such as wrinkles and the rough skin improving effect. did it.

─────────────────────────────────────────────────── ─── Continuation of front page (51) Int.Cl. ⁷ Identification code FI theme code (reference) A61K 35/78 A61K 35/78 W 45/00 45/00 F term (reference) 4C083 AA082 AA111 AA112 AB032 AB232 AB242 AC012 AC072 AC122 AC242 AC302 AC422 AC442 AC482 AC582 AC642 AC662 AC782 AD092 AD112 AD152 AD512 AD532 AD662 CC05 CC07 CC23 CC32 CC38 CC39 EE12 FF01 4C084 AA19 MA02 MA17 MA28 MA63 NA05 ZA891 4C088 AB02 AB12 AB26 AB47 MA50 MA62 MA08 MA59 AB08 NA14 ZA89

Claims (5)

[Claims] 1. A Salicaceae plant extract,
A skin external preparation containing a cell activating agent. Wherein Salicaceae (Salicaceae) extracts of plants is a water extract of Salicaceae (Salicaceae) plants, skin external preparation according to claim 1. 3. Salicaceae (Salicaceae) extracts of plants is an extract from the bark of Salicaceae (Salicaceae) plants, skin external preparation according to claim 1 or claim 2. 4. The external preparation for skin according to claim 1, wherein the extract of Salicaceae plant is Salix nigra L. extract. 5. The cell activator is ceramides and ceramide-like structure materials, yeast extract and yeast culture supernatant, pueraria root, Cordyceps sinensis, Ganoderma, the herbal extract of Amanin, asparagus (Asparagus) Genus, rosacea ( Epi
lobium , Avocado ( Persea americana Mil)
l.), aloe (Aloe) plants of the genus, apricot (Prunus armenia
ca L. var. ansu Maxim.), Ginkgo biloba
L.), Fagus japonica (Fagus japonica Maxim.), Giant garlic (Allium sativum L. f. Pekinense Makino ), Asian ginseng (Panax ginseng CAMeyer), chamomile (Matricaria chamomilla L.), yellowfin tuna (Phellodendro
n amurense Rupr.) and its cognate plant, Cucu
mis sativus L.), Calendula arvensis
L.), Getto ( Alpinia speciosa (Wendl.) K. Schu
m.), Shiitake mushroom ( Lentinusedodes Sing.), Cinnamon (Kiwi) ( Actinidia chinensis Planch.), Horsetail ( Equisetum arvense L.), Horse chestnut ( Ae )
sculus hippocastanum L.), garlic ( Allium )
sativum L.), Achillea apli
na L.; Achillea sibilica Ledeb.), assembly ( Swer
tia japonica Makino), Stephani
a cepharantha Hayata), Chisha (lettuce) ( Lactuca
sativa L.), swallow moto ( Clintonia udensis Traut
v. etMey.; Clintonia borealis ), Capsicum ( Caps )
icum annuum L.), Calendula offici
nalis L.), Houttuynia cordata Thun
b.), Horse Chestnut ( Aesculus turbinata Blume), Bukyou ( Matsuhod ) ( Poriacocos Wolf), Grape ( Vitis
vinifera L.), beech ( Fagus crenata Blume), loofah ( Luffa cylindrica M.Roemen), safflower ( Cartham )
us tinctorius L.), Mannenrou ( Rosmarinus offic )
inalis L.), citrus plant ( Citrus ), sucrose ( Sap)
indus mukurossi Gaertn.), purple ( Lithospermum )
officinale L. var. erythrorhizon Maxim.), Eucalyptus genus plant, lily ( Lilium ) genus plant, Eriophorum L. genus plant extract, hydroxy fatty acid and its salts and derivatives, nucleic acid and related substances , Proteins extracted from eggshell membranes, sericin and its hydrolysates, lactic acid bacteria extracts, bifidobacteria extracts, algae extracts, bittern components, vitamins A, vitamin B
₂ , vitamin B _{6 s} , nicotinic acid and its salts and derivatives, vitamin Ds such as ergocalciferol and cholecalciferol, vitamin Hs, spleen extract of mammals such as human and bovine, basic and acidic fibers The external preparation for skin according to any one of claims 1 to 4, which is one or more selected from blast cell growth factor and epidermal cell growth factor.