JP2003226632A - Skin cosmetic and food and beverage for beauty - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To obtain a collagen production promoter of a fibroblast of a dermic layer, which prevents and/or ameliorates skin aging, is an active oxygen scavenger, is a catalytic enzyme for converting super oxide into hydrogen peroxide, a radical scavenger, a hyaluronidase inhibitor and used for skin cosmetics, a food and a beverage for beauty as an active ingredient. <P>SOLUTION: The collagen production promoter, the active oxygen scavenger, the radical scavenger or the hyaluronidase inhibitor is obtained by extracting Pouzolzis zeylaniea of the family Urticaceae with water, a hydrophilic organic solvent or their mixture. The skin cosmetic and the food and beverage for beautification comprises the collagen production promoter or the like therein. <P>COPYRIGHT: (C)2003,JPO

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a collagen production promoter extracted from nettle family fog water kudzu, an active oxygen scavenger, a radical scavenger, a hyaluronidase inhibitor, and high aging of the skin containing them. The present invention relates to skin cosmetics and beauty foods and drinks having an improving effect.

[0002]

2. Description of the Related Art Active oxygen means oxygen that is more activated than normal oxygen and its related compounds, and is generated in the process of energy metabolism in living cells. Superoxide anion generated by electron reduction (.O ²⁻ ), hydrogen peroxide (H
4 types of ₂ O ₂ ), hydroxy radical (· OH), and singlet oxygen are called active oxygen.

These active oxygens are essential for the bactericidal mechanism of phagocytes and play an important role in the removal of viruses and cancer cells. However, excessive production of active oxygens causes the formation of membranes and tissues in vivo. It is thought that it attacks molecules in the body and decomposes, denatures or cross-links living tissues such as collagen, or oxidizes fats and oils to produce lipid peroxides that damage cells, and these disorders caused by active oxygen. However, it is considered to cause aging such as wrinkle formation of the skin and reduction of elasticity of the skin.

In this case, the excess superoxide is
It is sequentially eliminated by the catalytic action of intracellular superoxide dismutase (SOD), but this decrease in SOD action poses a problem, and SOD and SOD-like substances should be added to foods and beverages, skin cosmetics, and pharmaceuticals for ingestion and administration. Is being attempted.

It has been confirmed that glutathione, vitamins C, E, B ₆ , butylhydroxytoluene, butylhydroxyaniline and the like are effective as such substances having an active oxygen scavenging effect. There is a problem in stability and safety when blended in. Also, various natural products have been found as SOD-like substances (Japanese Patent Laid-Open No. 64-50877 and Japanese Patent Laid-Open No. 3-835).
48).

It is also known that free radicals generated in the living body react with lipids, nucleic acids, proteins and the like to damage cells and cause various diseases such as inflammation, aging, carcinogenesis and myocardial infarction. ing. Development of radical scavenging substances has been attempted for the purpose of preventing diseases caused by such radicals. Among them, green tea leaves (Japanese Patent No. 2085061) are used as radical scavengers derived from natural products.
No.), sesame seeds (Japanese Patent No. 3031844), and the like are known.

On the other hand, the epidermis and dermis of the skin are the epidermal cells,
It is composed of fibroblasts and extracellular matrix such as elastin, collagen, etc., which is outside these cells and supports the skin structure. In young skin, the water retention by interaction of these skin tissues maintains homeostasis,
Flexibility, elasticity, etc. are secured, and the appearance of the skin is firm and shiny, and is kept fresh.

In recent years, it has been pointed out that the matrix protease is particularly involved as a factor inducing this change. Among matrix-type proteases, collagenase, that is, MMP-1 (matrix metalloprotease) is known as an enzyme that decomposes type I and III collagen, which is a main constituent of the dermal matrix of skin, but its expression is not known. It is considered that it is greatly increased by the irradiation of ultraviolet rays, contributes to the decrease / denaturation of collagen by ultraviolet rays, and becomes a major factor such as the formation of wrinkles on the skin. Therefore, promotion of collagen production is important for preventing and improving skin aging.

[0009] Hyaluronidase is an enzyme that hydrolyzes hyaluronic acid. This hyaluronic acid is produced mainly by dermal fibroblasts, and is essential for improving the freshness, suppleness and luster of the skin. It is a glycoprotein that does not exist, but it decreases during the process of skin aging represented by aging. Therefore, it is considered that skin aging can be prevented if the promotion of hyaluronic acid production can be enhanced and the decrease in hyaluronic acid can be compensated.

[0010]

However, a collagen production promoter, an active oxygen scavenger, and a radical scavenger, which are excellent in safety and productivity and inexpensive, can prevent and improve high skin aging. , And the demands of consumers for hyaluronidase inhibitors are extremely strong, and it is the current situation that the ones which are sufficiently satisfactory are not yet provided.

Under such circumstances, the present invention aims to solve various problems in the prior art and achieve the following objects. That is, the present invention provides a collagen production promoter capable of promoting collagen production in dermal fibroblasts to prevent and / or ameliorate skin aging, and active oxygen which is a catalytic enzyme for converting superoxide into hydrogen peroxide. Scavenging action, radical scavenger having radical scavenging action, hyaluronidase inhibitor having hyaluronidase activity inhibitory action, and skin cosmetics and beauty foods and drinks containing these active ingredients and having high skin aging prevention / improvement action The purpose is to provide.

[0012]

Means for Solving the Problems The inventors of the present invention have conducted extensive studies to solve the above-mentioned problems, and as a result, the extract of nettle family fog water kudzu has a collagen production promoting action, an active oxygen scavenging action and a radical scavenging action. The present invention finds that skin cosmetics and beauty foods and drinks that have both action and hyaluronidase activity inhibitory action and that contain these active ingredients can prevent and / or improve skin aging with high safety and excellent, and the present invention Came to make.

Accordingly, the present invention provides the following collagen production promoter, active oxygen scavenger, radical scavenger, hyaluronidase inhibitor, skin cosmetics and cosmetic foods.

According to the invention of claim 1,
A collagen production promoting agent comprising as an active ingredient a collagen production promoting substance obtained by extraction with water, a hydrophilic organic solvent or a mixed solvent thereof. The invention according to claim 2 comprises an active oxygen scavenger as an active ingredient, which contains an active oxygen scavenging substance obtained by extracting the nettle mist water mist with water, a hydrophilic organic solvent or a mixed solvent thereof. Is. The invention of claim 3 is
It is a radical scavenger characterized by containing a radical scavenging substance obtained by extracting the nettle mist water mist with water, a hydrophilic organic solvent or a mixed solvent thereof as an active ingredient. The invention of claim 4 is a hyaluronidase inhibitor characterized in that it contains, as an active ingredient, a hyaluronidase activity-inhibiting substance obtained by extracting nettle mist water mist with water, a hydrophilic organic solvent or a mixed solvent thereof. is there. In the invention of claim 5, the hydrophilic organic solvent is selected from the group consisting of methanol, ethanol, propanol, 1,3-butylene glycol, glycerin and propylene glycol. It is an oxygen scavenger, a radical scavenger, or a hyaluronidase inhibitor. A sixth aspect of the present invention is a skin cosmetic containing the active ingredient extracted from the nettle mist water fodder according to any one of the first to fifth aspects. The invention of claim 7 further comprises one or more whitening agents selected from the group consisting of ascorbic acid and its derivatives, placenta extract, kojic acid, rucinol, arbutin, ellagic acid and chamomile extract. The skin cosmetic composition according to item 6. The invention of claim 8 is a food and drink product for cosmetics, characterized in that it contains an active ingredient extracted from the mist water kudzu of the nettle family according to any one of claims 1 to 5.

In addition, the extract of fog water kudzu of the nettle family
It has not been known at all that it has a collagen production promoting action, an active oxygen scavenging action, a radical scavenging action, and a hyaluronidase activity inhibiting action, is excellent in safety, and is effective in preventing and / or improving skin aging. Is a new finding of the present inventors.

[0016]

BEST MODE FOR CARRYING OUT THE INVENTION The present invention will be described in more detail below. The collagen production promoter, active oxygen scavenger, radical scavenger and hyaluronidase inhibitor of the present invention are
The active ingredient contains a collagen production promoting agent, an active oxygen scavenger, a radical scavenger, and a hyaluronidase inhibitor which are extracted from the nettle family Musi Kutsuka.

[0017] The above-mentioned fossil watermelon is a nettle family plant, also known as Pleurotus ostreatus, and is a perennial herb with stems that are hairless or sparse. This fog of water is Japan,
It grows naturally in fields, roadsides, etc. in China, India, etc. and can be easily obtained. In the present invention,
The above-ground parts such as leaves and stems of fog water kudzu are used as the extraction raw material.

The details of the collagen production promoting agent, active oxygen scavenger, radical scavenger, and hyaluronidase activity inhibitor contained in the above nettle mist water kudzu are unknown, but using the above-mentioned raw materials for extraction, It can be obtained by an extraction method generally used for extraction. In addition, an extract, a diluted solution of the extract, a dried product obtained by drying the extract, or a crudely purified product or a purified product thereof is included.

For example, it can be obtained by drying the above-ground part of the nettle mist water kudzu and then subjecting it to solvent extraction as it is or after crushing with a crusher. Drying may be performed in the sun or using a commonly used dryer. Further, the aerial part of the fog water kudzu may be used as an extraction raw material after pretreatment such as degreasing with a non-polar solvent such as hexane or benzene. By performing a pretreatment such as degreasing, the extraction treatment can be efficiently performed.

As the solvent used for the extraction, it is preferable to use water, a hydrophilic organic solvent or a mixed solution thereof at a temperature from room temperature to the boiling point of the solvent or lower.

The water that can be used as the extraction solvent includes pure water, tap water, well water, mineral water, mineral water, hot spring water, spring water, fresh water and the like, as well as those that have been subjected to various treatments. Examples of the treatment applied to water include purification, heating, sterilization, sterilization, filtration, ion exchange, adjustment of osmotic pressure, buffering, and the like. Therefore, the water that can be used as the extraction solvent also includes purified water, hot water, ion-exchanged water, physiological saline, phosphate buffer, phosphate buffered saline and the like.

Examples of the hydrophilic organic solvent include lower alcohols having 1 to 5 carbon atoms such as methanol, ethanol, propyl alcohol and isopropyl alcohol; lower aliphatic ketones such as acetone and methyl ethyl ketone;
1,3-butylene glycol, propylene glycol,
2 to 2 carbon atoms such as isopropylene glycol and glycerin
The polyhydric alcohol of No. 5 and the like can be mentioned, and a mixed solvent of these hydrophilic organic solvent and water can be used. When a mixed solvent of water and a hydrophilic organic solvent is used, in the case of a lower alcohol, 1 part is added to 10 parts by mass of water.
To 90 parts by mass, 1 to 40 parts by mass for 10 parts by mass of water in the case of lower aliphatic ketone, and 1 part of water in the case of polyhydric alcohol.
It is preferable to add 1 to 90 parts by mass to 0 part by mass.

In the present invention, it is not necessary to adopt a special extraction method for extracting the collagen production promoting substance, the active oxygen scavenging substance, the radical scavenging substance, and the hyaluronidase activity-inhibiting substance from the nettle mist, and it is not necessary to use a special extraction method at room temperature or It can be extracted using any device under reflux heating.

Specifically, the above-ground part of the nettle family fog water kudzu is put into a treatment tank filled with an extraction solvent, and the mixture is left standing for 30 minutes to 2 hours with occasional stirring to dissolve soluble components. After elution, the solid matter is removed by filtration, the extraction solvent is distilled off from the obtained extract, and the extract is obtained by drying. The amount of the extraction solvent is usually 5 to 15 times the amount of the extraction raw material (mass ratio), and the extraction conditions are usually 50 to 95 ° C. and about 1 to 4 hours when water is used as the extraction solvent. Further, when a mixed solvent of water and ethanol is used as the extraction solvent, it is usually at 40 to 80 ° C. for about 30 minutes to 4 hours. The extract obtained by extracting with a solvent can be blended as it is as an active ingredient of the present invention as long as the extract solvent is highly safe, and can be used as a concentrated solution or a dried product thereof. Things are easier to use.

The obtained extract is a diluent or concentrate of the extract, a dried product of the extract, or a crudely purified product or a purified product of the extract, within the range not causing a decrease in physiological activity. According to a conventional method, treatments such as dilution, concentration, drying and purification may be performed. The purification can be carried out by, for example, activated carbon treatment, adsorption resin treatment, ion exchange resin treatment or the like.

The obtained extract can be used as it is as a collagen production promoter, an active oxygen scavenger, a radical scavenger, and a hyaluronidase inhibitor.
Concentrated liquid or dried product is easier to use,
A pharmaceutically acceptable carrier such as dextrin or cyclodextrin for facilitating storage and handling,
Other optional auxiliaries can be added. In addition, the fog-water kudzu extract may be formulated into a desired form such as powder, granules, tablets, etc. by a conventional method.

<Skin cosmetics> Collagen production promoters, active oxygen scavengers, radical scavengers, and hyaluronidase inhibitors obtained from the fog water kudzu extract can prevent and / or improve skin aging. At the same time, since it is excellent in the feeling of use and safety when applied to the skin, it is suitable to be blended in a skin cosmetic. The amount of the collagen production promoter, active oxygen scavenger, radical scavenger, and hyaluronidase inhibitor blended into the skin cosmetics can be appropriately adjusted depending on the type of skin cosmetics and the physiological activity of the extract, etc. A suitable blending ratio is about 0.01 to 10% by mass based on the total amount of the skin cosmetics, calculated as a standard fog water kudzu extract.

The skin cosmetic of the present invention preferably further contains a whitening agent in an amount of 0.0001 to 10% by mass, particularly 0.1 to 5% by mass based on the total amount of the skin cosmetic.

Examples of the whitening agent include ascorbic acid or its derivative, sulfur, placenta hydrolyzate, ellagic acid or its derivative, kojic acid or its derivative, glucosamine or its derivative, arbutin or its derivative, hydroxycinnamic acid or Derivatives thereof, glutathione, chamomile extract, placenta extract, lucinol, arnica extract, sardine extract, sow syrup extract, psycho extract, bow-wow extract, mannula mushroom mycelium culture or extract thereof, linden extract, peach leaf extract, age extract, Kujin extract, Chinese cabbage extract, Toki extract, Yokuinin extract, Oyster leaf extract, Rhizoma extract, Panpis extract, Hamamelis extract, Horse chestnut extract, Hypericum perforatum extract, Oil-soluble licorice extract (liquorice hydrophobic flavone, grab) Gin, glabrene, lycochalcone A) and the like. Among them, from the viewpoint of improving the whitening effect, one selected from ascorbic acid and its derivatives, placenta extract, kojic acid, rucinol, arbutin, ellagic acid and chamomile extract. Alternatively, it is preferable to use two or more kinds.

In the skin cosmetic of the present invention, there is no particular limitation as to what can be used as an optional component together with the collagen production promoter, active oxygen scavenger, radical scavenger, hyaluronidase inhibitor, and whitening agent. , Which is appropriately selected according to the purpose, for example, astringent, sterilization /
Antibacterial agents, ultraviolet absorbers, humectants, cell activators, anti-inflammatory / anti-allergic agents, oils and fats, waxes, hydrocarbons, fatty acids, alcohols, esters, surfactants, fragrances and the like are used. When these optional components are used in combination with the collagen production promoter, active oxygen scavenger, radical scavenger, and hyaluronidase inhibitor, they act synergistically to bring about an excellent use effect more than normally expected. There is.

Examples of the astringents include citric acid or salts thereof, tartaric acid or salts thereof, lactic acid or salts thereof,
Aluminum chloride, aluminum sulfate / potassium sulfate, allantoin chlorohydroxyaluminum, allantoin dihydroxyaluminum, zinc paraphenol sulfonate, zinc sulphate, juniper extract, age extract, hamamelis extract, gem ginger extract, chacatechins,
Odorikosu extract, Hypericum extract, Rheum palmatum extract, Cornflower extract, Ivy extract, Cucumber extract, Horse chestnut extract, Salvia extract, Melissa extract, etc. are mentioned, and one of these may be used alone or 2
A combination of two or more species can be used.

Examples of the bactericidal / antibacterial agents include benzoic acid, sodium benzoate, paraoxybenzoic acid ester, distearylmethylammonium chloride, benzethonium chloride, chlorhexidine hydrochloride, photosensitizer No. 101, photosensitizer No. 201, salicylic acid, salicylic acid. Sodium, sorbic acid, halocarban, resorcin, parachlorophenol, phenoxyethanol, bisabolol, hinokitiol, menthol, chitosan, degradation products of chitosan, diyu extract, kujin extract, nematophor extract, loquat extract, yucca extract, aloe extract, cinnamon extract, zedoary extract, etc. These may be used alone or in combination of two or more.

Examples of the ultraviolet absorber include β-
Isopropylfuranone derivative, urocanic acid, ethyl urocanate, oxybenzone, oxybenzone sulfonic acid, tetrahydroxybenzophenone, dihydroxydimethoxybenzophenone, dihydroxybenzophenone, synoxate, methyl diisopropylcinnamate, octyl methoxycinnamate, glyceryl paraaminobenzoate, paradimethylaminobenzoic acid Amyl, octyl paradimethyl benzoate, paraaminobenzoic acid, ethyl paraaminobenzoate, titanium oxide, β-carotene, γ-oryzanol, rice bran extract, aloe extract, birch extract, birch extract, chamomile extract, henna extract, butterfly ginger extract, ginkgo biloba. Leaf extract, chamomile extract, hawthorn extract, oil-soluble licorice extract, etc. These 1 type can be used individually or in combination of 2 or more types.

Examples of the moisturizers include serine, glycine, threonine, alanine, collagen, hydrolyzed collagen, hydronectin, fibronectin, keratin, elastin, royal jelly, chondroitin sulfate heparin, glycerophospholipid, glyceroglycolipid, sphingophospholipid. , Glycosphingolipids, linoleic acid or its esters, eicosapentaenoic acid or its esters, pectin, bifidobacteria fermented product, lactic acid fermentation product, yeast extract, litchi mycelium culture or its extract, wheat germ oil, avocado Oil, rice germ oil, jojoba oil, soybean phospholipids, γ-oryzanol, velvet aoi extract, Yokuinin extract, dio extract, tannin extract, tannin extract, kidachi aloe extract, burdock extract, mannen wax extract, alni. Extract, wheat bran and the like, may be used these alone or in combination of two or more.

Examples of the cell activating agent include riboflavin or its derivative, pyridoxine or its derivative,
Nicotinic acid or a derivative thereof, pantothenic acid or a derivative thereof, α-tocopherol or a derivative thereof, arnica extract, carrot extract, rapeseed ginseng extract, loofah extract (saponin), shikon extract, oak extract, button pie extract, peony extract, mukurodi extract, safflower Extracts, ashitaba extract, loquat leaf extract, Hiki-Oshi extract, Yukinoshita extract, yellow-eye extract, salvia extract, garlic extract, mannen wax extract and the like can be used, and one of these can be used alone or in combination of two or more. .

Examples of the anti-inflammatory / anti-allergic agents include azulene, allantoin, aminocaproic acid, indomethacin, lysozyme chloride, epsilon aminocaproic acid, oxybenzone, glycyrrhizic acid or its derivative, glycyrrhetinic acid or its derivative, and photosensitizer 301.
No., Photosensitizer No. 401, diphenhydramine hydrochloride, tranexamic acid or its derivative, adenosine phosphate, estradiol, estrone, ethinyl estradiol, cortisone, hydrocortisone, prednisone, progesterone, corticosterone, arnica extract, inchinko extract, sanshishi extract, deer yak. Examples of the extract include licorice extract, citrus extract, mugwort extract, oleander extract, gentian extract, psychological extract, senkyu extract, yarrow extract, laurel extract, and perilla extract, and one of these may be used alone or 2
A combination of two or more species can be used.

Examples of the oils and fats include soybean oil, linseed oil, sesame oil, bran oil, cottonseed oil, rapeseed oil, safflower oil, corn oil, olive oil, camellia oil, almond oil, castor oil, peanut oil, cocoa oil. , Palm kernel oil,
Beef tallow, mink oil, jojoba oil, evening primrose oil, horse oil, etc. are mentioned, and these 1 type can be used individually or in combination of 2 or more types.

Examples of the waxes include carnauba wax, candelilla wax, bees wax, honey bees wax, spermaceti, cerax, and lanolins, and one of these may be used alone or in combination of two or more. Can be used.

Examples of the above-mentioned hydrocarbons include liquid paraffin, petrolatum, microthristalin wax,
Ceresin, squalane, polyethylene powder and the like can be mentioned, and one of these can be used alone or in combination of two or more.

Examples of the fatty acids include stearic acid, linoleic acid, lauric acid, myristic acid, palmitic acid, hevenic acid, lanolinic acid, oleic acid, undecylenic acid, isostearic acid, etc. They can be used alone or in combination of two or more.

Examples of the alcohols include lauryl alcohol, cetyl alcohol, stearyl alcohol, lanolin alcohol, hydrogenated lanolin alcohol,
Oleyl alcohol, hexadecyl alcohol, 2-octyldodecanol, glycerin, sorbitol, propylene glycol, 1,3-butylene glycol, ethylene glycol or a polymer thereof, glucose, sucrose, cholesterol, phytosterol, cetostearyl alcohol and the like, These 1 type can be used individually or in combination of 2 or more types.

Examples of the esters include decyl oleate, butyl stearate, myristyl myristate, hexyl laurate, isopropyl palmitate, isopropyl myristate, octyldodecyl myristate, hexyldecyl dimethyloctanoate, propylene glycol dioleate. , Diethyl phthalate, glyceryl monostearate, glyceryl trimyristate, cetyl lactate and the like, and one of these may be used alone or two or more thereof may be used in combination.

As the above-mentioned surfactant, for example, nonionic surfactants, anionic surfactants, cationic surfactants, amphoteric surfactants and the like can be used, but among them, skin diseases do not occur. , Or a surfactant listed on the basis of slight cosmetic raw materials is preferable. Specifically, soybean lecithin, egg yolk lecithin, saponin, oligoglycoside, phospholipid biosurfactant, acyl peptide biosurfactant, polyoxyethylene polyoxypropylene glycol, polyoxyethylene polyoxypropylene cetyl ether, polyoxyethylene. Polyoxypropylene butyl ether, polyoxyethylene coconut oil fatty acid monoethanolamide, polyoxyethylene lauryl ether, polyoxyethylene lauryl ether sulfate triethanolamine, sodium polyoxyethylene lauryl ether sulfate, polyoxyethylene lauryl ether phosphate, polyoxyethylene Sodium lauryl ether phosphate, polyoxyethylene lanolin, polyoxyethylene lanolin alcohol, polyoxyp Pyrene butyl ether, polyvinyl alcohol, polyvinylpyrrolidone, sodium polyphosphate, sorbitan monooleate, polyethylene glycol monooleate, polyoxyethylene sorbitan monooleate, ethylene glycol monostearate, sorbitan monostearate, propylene glycol monostearate, mono Polyethylene glycol stearate, polyoxyethylene glycerin monostearate, polyoxyethylene sorbitan monostearate, sorbitan monopalmitate, polyoxyethylene sorbitan monopalmitate, sorbitan monolaurate, polyethylene glycol monolaurate, polyoxyethylene sorbitan monolaurate, Monolauric acid polyoxyethylene sorbit, coconut oil and fat Surfactants such as acid diethanolamide and the like, alone these one or
A combination of two or more species can be used.

Examples of the fragrance include menthol,
Carvone, eugenol, anethole, peppermint oil, spearmint oil, peppermint oil, eucalyptus oil, anise oil and the like can be mentioned, and one of these can be used alone or in combination of two or more.

The skin cosmetic of the present invention has an excellent effect of preventing and / or improving skin aging.
Ointment, cream, emulsion, lotion, lotion, pack,
It can be widely used as lipsticks, lip balms, bath salts and the like.

<Beauty Foods and Beverages> It can be confirmed that the active ingredients of the fog water kuzu extract, collagen production promoting substances, active oxygen scavengers, radical scavengers, and hyaluronidase inhibitors are not digested in the digestive tract. Therefore, it can be added to any food or drink or dietary supplement to prevent skin aging through collagen production promoting action, active oxygen scavenging action, radical scavenging action, and hyaluronidase activity inhibiting action when these are ingested and / Or it can be improved. In the present invention, the "cosmetic food and drink" means a wide range of foods and drinks for the purpose of preventing and improving skin aging.

The above-mentioned cosmetic food and drink is the above-mentioned collagen production promoter, active oxygen scavenger, radical scavenger, and hyaluronidase inhibitor compounded in any food or drink that does not interfere with its activity. Alternatively, it may be a granular or tablet nutritional supplement containing the collagen production promoting substance, active oxygen scavenger, radical scavenger, and hyaluronidase inhibitor of the present invention as main components.

When a cosmetic food or drink is prepared by blending the collagen production promoter, active oxygen scavenger, radical scavenger, and hyaluronidase inhibitor of the present invention, sugars such as dextrin and starch; gelatin; Proteins such as soybean protein and corn protein; alanine,
Amino acids such as glutamine and isoleucine; polysaccharides such as cellulose and gum arabic; oils and fats such as soybean oil and medium-chain fatty acid triglyceride;

The content of the collagen production promoter, active oxygen scavenger, radical scavenger, and hyaluronidase inhibitor in the cosmetic food or drink of the present invention should be the same as that for adults considering the general intake of the food or drink to be added. It is preferable to adjust the intake amount of the extract per day to about 1 to 1000 mg per day.

The beauty food or drink is not particularly limited, but specific examples thereof include soft drinks, carbonated drinks,
Beverages such as nutritional drinks, fruit drinks, lactic acid drinks (including concentrated liquids and powders for adjustment of these drinks); ice creams, ice sorbets, shaved ice, and other frozen desserts; buckwheat, udon, harusame, gyoza skin, Shumai skin, Noodles such as Chinese noodles and instant noodles; candy, candy, gum, chocolate, snacks, biscuits, jellies, jams, creams,
Confectioneries such as baked goods; processed fish and livestock products such as kamaboko, ham and sausage; dairy products such as processed milk and fermented milk; oils and fats such as salad oil, tempura oil, margarine, mayonnaise, shortening, whipped cream, dressings Processed foods; seasonings such as sauces and sauces; health and nutritional supplements in various forms such as tablets and granules; and other soups, stews, salads, prepared foods, pickles, and the like.

The collagen production promoter, active oxygen scavenger, radical scavenger, hyaluronidase inhibitor, and the skin cosmetics and cosmetic foods and drinks containing the same according to the present invention are preferably applied to humans. However, it is not particularly limited and can be applied to animals other than humans.

[0052]

EXAMPLES The present invention will be described in more detail below with reference to production examples and examples, but the present invention is not limited to the following examples.

[Production Example 1] Dried product 30 from the above-ground part of fog water kudzu
0 g corresponds to No. 1 shown in Table 1. 1-3 extraction solvent 2000
Pour into mL, hold at 70 ° C for 3 hours with gentle stirring, then filter and concentrate the filtrate at 40 ° C under reduced pressure.
Sample No. 1-3 fog water kudzu extracts were obtained. The yield of each extract was as shown in Table 1.

[0054]

[Table 1]

Example 1 Collagen production promoting action test
Test Sample No. of Production Example 1 About 1-3, Webster
Et al. (Anal. Biochem., Vol. 9).
6,220,1979). Specifically, the test was conducted as follows.

First, human fibroblasts were seeded on a 24-well plate, and each sample-added medium (sample concentration: 50 ppm, 12. ppm) at 37 ° C. under 5% CO ₂ -95% air.
After culturing at 5 ppm) for several days, β-aminopropionitrile and [ ³ H] -proline were added, and the cells were further cultured for 24 hours. A pepsin / acetic acid solution was added to the entire culture solution and digested at 4 ° C. for 16 hours. Next, a carrier was added to this digestive solution to precipitate it with a 0.7 mol / L saline solution, and the solution was redissolved under neutral conditions to give 4.2 mol / L.
It was reprecipitated with a saline solution. After washing the obtained precipitate with 20% ethanol, the radioactivity of the precipitate was measured. The collagen production accelerating rate was calculated assuming that the radioactivity when no sample was added was 100%. Table 2 shows the collagen production promotion rate (%) of each sample.

[0057]

[Table 2] From the results of Table 2, it was confirmed that the extract of fog water kudzu had an action of promoting collagen production of fibroblasts.

Example 2 Superoxide-like activity
Action test (NBT method) Sample No. 1 of Production Example 1 For 1-3, the superoxide scavenging effect was tested. Specifically, the test was conducted as follows.

3 mmol / L xanthine, 0.05 m
ol / L Na ₂ CO ₃ buffer (pH 10.2), 3 m
mol / L EDTA, BSA solution, and 0.75 mm
Each 0.1 mL of ol / L NBT was placed in a test tube, 0.1 mL of each sample solution (solvent: DMSO + water) was added thereto, and the mixture was allowed to stand at 25 ° C. for 10 minutes. Next, add a xanthine oxidase solution (enzyme solution) and stir quickly.
It was allowed to stand at 5 ° C for 20 minutes. Then, 0.1 mL of 6 mmol / L copper chloride was added to stop the reaction, and the wavelength was 560 n.
The absorbance at m was measured. The same operation and the measurement of the absorbance were performed without adding the enzyme solution. Furthermore, the same measurement was performed when distilled water was added without adding the sample solution.

From the above measurement results, the superoxide scavenging rate (%) was calculated by the following mathematical formula 1. <Equation 1> Superoxide erasing rate (%) = [1- (AB) /
(C−D)] × 100 where A means the absorbance when the enzyme solution was added and the sample solution was added, B means the absorbance when the enzyme solution was not added and the sample solution was added, and C is the enzyme solution added and the sample was added. It means the absorbance when no solution was added, and D means the absorbance when no enzyme solution was added and no sample solution was added.

Next, the erasure rate was measured by gradually reducing the sample concentration, and the erasure rate of superoxide was 5%.
The sample concentration IC ₅₀ (ppm) at 0% was determined by the interpolation method (the smaller the IC ₅₀ value, the stronger the superoxide scavenging action). The results are shown in Table 3.

[0062]

[Table 3] From the results of Table 3, it was confirmed that the mist water kudzu extract had a strong SOD-like activity.

Example 3 DPPH Elimination Action Test Sample No. 1 of Production Example 1 For 1-3, DPPH extinction effect was tested. Specifically, the test was conducted as follows.

1.5 × 10 ⁻⁴ mol / L DPPH
(1,1-Diphenyl-2-picryhydr
3 mL of each sample solution was added to 3 mL of the azyl) ethanol solution, the container was immediately sealed and shaken, and the mixture was allowed to stand for 30 minutes. Then, the absorbance at a wavelength of 520 nm was measured. As a control, the same operation was performed using a solvent in which the sample solution was dissolved instead of the sample solution, and the absorbance at a wavelength of 520 nm was measured. Further, as a blank, 3 mL of the sample solution was added to ethanol, and immediately after that, the absorbance at a wavelength of 520 nm was measured.

From the above measured absorbances, the following mathematical formula 2
The radical scavenging rate was calculated by. <Formula 2> DPPH elimination rate (%) = {1- (B−C) / A} × 100 where A means the absorbance of the control, B means the absorbance when the sample solution is added, and C Means the absorbance of the blank.

Next, the scavenging rate was measured by gradually decreasing the sample concentration, and the scavenging rate of DPPH-based radicals was 5%.
The sample concentration IC ₅₀ (ppm) at 0% was obtained by the interpolation method (the smaller the IC ₅₀ value, the stronger the DPPH erasing action). The results are shown in Table 4.

[0067]

[Table 4] From the results in Table 4, it was confirmed that the mist water kudzu extract had a scavenging effect on DPPH radicals.

[Example 5] Hyaluronidase inhibitory action
Test Sample No. 1 of Production Example 1 For 1-3, the hyaluronidase inhibitory effect was tested. Specifically, the test was conducted as follows.

Hyaluronidase solution (400 units /
(mL, pH 3.5 acetate buffer) 0.1 mL and 0.2 mL of each sample solution were mixed and heated to 37 ° C. for 20 minutes,
Activator solution (2.5 mmol / L CaCl ₂ ) 0.
2 mL was added and the enzyme was activated by heating at 37 ° C. for 20 minutes. Add 0.5 mL of potassium hyaluronate buffer,
After reacting at 37 ° C. for 40 minutes, 0.4 N sodium hydroxide (0.2 mL) was added and the mixture was ice-cooled to stop the reaction.

Then, 0.2 mL of 0.8 mol / L boric acid solution (pH 9.1) was added, and after heating in a boiling water bath for 3 minutes, the mixture was immediately ice-cooled for 10 minutes. DMAB reagent (p-
Dimethylaminobenzaldehyde (10 g) was dissolved in a mixed solution of 10 N hydrochloric acid (12.5 mL) and acetic acid (87.5 mL) and diluted 10 times with acetic acid (6.0 mL), and the mixture was added at 37 ° C.
The N released by the above enzyme reaction
-Acetylglucosamine was developed and the absorbance at a wavelength of 585 nm was measured. As a control, the same operation and absorbance measurement were performed when water was added instead of the sample solution. From the above measurement results, the hyaluronidase activity inhibition rate (%) was calculated by the following mathematical formula 3. <Equation 3> Hyaluronidase activity inhibition rate (%) = [1- (AB)
/ (C−D)] × 100 where A means the absorbance when the sample solution was added and the enzyme was added, B means the absorbance when the sample solution was added and the enzyme was not added, and C was the sample not added and the enzyme was added. Means the absorbance at the time (control), and D means the absorbance at the time when the sample is not added and the enzyme is not added (control).

Next, the hyaluronidase activity inhibition rate was measured by changing the concentration of the sample solution stepwise.
The sample concentration IC _{50 at} which 0% was obtained was obtained by the interpolation method (I
C for about _{5 0} value is less strong hyaluronidase inhibitory effect). The results are shown in Table 5.

[0072]

[Table 5] From the results in Table 5, it was confirmed that the mist water kudzu extract had a hyaluronidase inhibitory action.

[Example 6] Rough skin improving effect (aging of the skin
Antioxidation / Ameliorating Action) Test An emulsion having the following composition (hereinafter referred to as “the emulsion of the present invention”) containing the fog water kudzu extract (Sample No. 1) of Production Example 1 was prepared according to a conventional method.

[0074] <Inventive emulsion>   Mist water kudzu extract (Sample No. 1) 1 g   Cetyl alcohol 0.5g   2.0 g of beeswax   Polyoxyethylene sorbitan oleate (10 EO) 1.0 g   Glyceryl monostearate 1.0 g   Hyaluronic acid 0.1g   Propylene glycol 5.0g   Ethanol 3.0g   Methyl paraoxybenzoate 0.3g   Fragrance 0.03g   Purified water balance (total volume is 100 mL)

The following evaluation tests were carried out on the above-described emulsion of the present invention and a comparative emulsion having the same composition as the emulsion of the present invention except that it did not contain fog-water kudzu extract.

<Evaluation test> Subjects: Among a large number of women aged 22 to 43 years old, the sulcus and crust disappeared, and a wide range of keratin was turned over (evaluation shown in Table 6 was 1), or sulcus and skin. Twenty people who were judged to have rough skin with unclear hills and partial keratinization (evaluation shown in Table 6 was 2) were selected as test subjects.

<Application Test> For each test subject, the emulsion of the present invention was applied to the right half of the face, and the comparative emulsion was applied to the left half of the face, 30 times each in the morning and evening.
It was applied for a day.

[Judgment 1: Skin Roughness Improving Effect] After the coating test, Silflo (FLEXICLEVELOPMEN)
A replica of the face was taken using a replica method by TS LTD), the state of skin print and the state of exfoliation of keratin were observed with a microscope of 50 times, and the state of skin was judged according to the evaluation criteria shown in Table 6. The judgment results are shown in Table 7.

[0079]

[Table 6]

[0080]

[Table 7] From the results shown in Table 7, the area to which the emulsion of the present invention was applied was significantly rougher than the area to which the comparative emulsion was applied (skin aging).
Was confirmed to be improved.

[Determination 2 / Sensory Evaluation] Regarding the feeling of use and the effect on the skin, all subjects were asked about the superiority or inferiority of the emulsion of the present invention and the comparative emulsion. Table 8 shows the results of collecting the answers.

[0082]

[Table 8] From the results in Table 8, the same effects as those of the above-described Judgment 1 and excellent feeling of use could be confirmed by the sensory evaluation.

Therefore, from the results of judgments 1 and 2, the skin cosmetics containing the mist and water kudzu extract have an effect of preventing and improving skin aging (effect of improving rough skin), and have a feeling of use when applied to the skin. It was confirmed that it was excellent in safety.

Example 7 Emulsion An emulsion having the following composition was produced by an ordinary method. Jojoba oil 4 g Olive oil 2 g Squalane 2 g Cetanol 2 g Glyceryl monostearate 2 g Polyoxyethylene cetyl ether (20 EO) 2.5 g Polyoxyethylene sorbitan oleate (20 EO) 2 g 1,3-butylene glycol 3 g Paraoxybenzoic acid Methyl 0.15g Perfume 0.05g Mist water kudzu extract (Sample No. 1 of Production Example 1) 1g Purified water balance (total amount is 100g)

Example 8 Lotion A lotion having the following composition was produced by a conventional method. Glycerin 3 g 1,3-butylene glycol 3 g Polyoxyethylene sorbitan oleate (20 EO) 0.5 g Methyl paraoxybenzoate 0.15 g Citric acid 0.1 g Sodium citrate 0.1 g Perfume 0.05 g Fog water kudzu extract (Sample No. 2 of Production Example 1) 2 g Purified water balance (total amount is 100 g)

Example 9 Cream A cream having the following composition was produced by a conventional method. Liquid paraffin 5 g Salix beeswax 4 g Cetanol 3 g Squalane 10 g Lanolin 2 g Stearic acid 1 g Polyoxyethylene sorbitan oleate (20 EO) 1.5 g Glyceryl monostearate 3 g 1,3-Butylene glycol 6 g Methyl paraoxybenzoate 1.5 g Perfume 0 .1 g mist water kudzu extract (Sample No. 3 of Production Example 1) 1 g purified water balance (total amount is 100 g)

Example 10 Pack A pack having the following composition was produced by a conventional method. Polyvinyl alcohol 15 g Polyethylene glycol 3 g Propylene glycol 7 g Ethanol 10 g Ethyl paraoxybenzoate 0.05 g Perfume 0.05 g Mist water kudzu extract (Sample No. 1 of Production Example 1) 5 g Purified water balance (total amount is 100 g)

Example 11 Ame Candy A candy was produced from the following raw materials by a conventional method. Sucrose 70 parts by weight starch syrup 30 parts by weight citric acid 1 part by weight fragrance 0.1 parts by weight fog water kudzu extract (Sample No. 1 of Production Example 1) 1 part by weight

Example 12 Chewing Gum Chewing gum was produced from the following raw materials by a conventional method. Chewing gum base 70 parts by mass Sucrose 54 parts by mass Syrup 20 parts by mass Softener 4 parts by mass Fragrance (mint oil) 1 part by mass Fog and water extract (Sample No. 3 of Production Example 1) 1 part by mass

Example 13 Tablet Dietary Supplement The following mixture was tabletted to produce a tablet dietary supplement. Fog and water extract (Sample No. 2 of Production Example 1) 50 parts by mass Powdered sugar (sucrose) 188 parts by mass Glycerin fatty acid ester 12 parts by mass

Example 14 Granular Dietary Supplement The following mixture was formed into granules to produce a granular dietary supplement. Mist water kudzu extract (Sample No. 1 of Production Example 1) 30 parts by mass Beet oligosaccharide 1000 parts by mass Vitamin C 167 parts by mass Stevia extract 10 parts by mass

The skin cosmetics and beauty foods and drinks of Examples 7 to 14 all have excellent collagen production promoting action, active oxygen scavenging action, radical scavenging action and hyaluronidase activity inhibiting action, and are effective for skin aging. It was useful for prevention and / or improvement.

[0093]

As described above, according to the present invention,
Collagen production promoter capable of promoting collagen production in dermal fibroblasts and preventing and / or improving skin aging; active oxygen having active oxygen scavenging action which is a catalytic enzyme for converting superoxide into hydrogen peroxide A scavenger, a radical scavenger, and a hyaluronidase inhibitor having a hyaluronidase activity inhibitory action can be obtained.

Further, according to the present invention, the skin containing the collagen production promoter, active oxygen scavenger, radical scavenger, and hyaluronidase inhibitor is extremely useful for preventing and / or improving aging of the skin. Cosmetics and beauty foods and drinks can be obtained.

─────────────────────────────────────────────────── ─── Continuation of front page (51) Int.Cl. ⁷ Identification code FI theme code (reference) A61K 7/00 A61K 7/00 K C12N 9/99 C12N 9/99 // A61K 35/78 A61K 35/78 C A61P 43/00 111 A61P 43/00 111 (72) Inventor Masazumi Miyakoshi 1089-8 Shinkata-cho, Ashina-gun, Hiroshima Prefecture Maruzen Pharmaceutical Co., Ltd. Research Institute F-term (reference) 4B018 MD61 ME06 MF01 4C083 AA071 AA082 AA111 AA112 AA122 AC022 AC072 AC102 AC122 AC182 AC242 AC302 AC311 AC392 AC442 AC482 AC841 AD042 AD112 AD332 AD391 AD512 AD641 BB41 CC04 CC05 CC07 EE12 EE16 4C088 AB12 BA08 BA09 BA10 MA52 MA63 NA14 ZA89

Claims (8)

[Claims] 1. A collagen production-promoting agent comprising as an active ingredient a collagen production-promoting substance obtained by extracting a nettle mist water fodder with water, a hydrophilic organic solvent or a mixed solvent thereof. 2. An active oxygen scavenger containing an active oxygen scavenging substance obtained as an active ingredient, which is obtained by extracting a nettle mist water mist with water, a hydrophilic organic solvent or a mixed solvent thereof. 3. A radical scavenger comprising a radical scavenging substance obtained as an active ingredient, which is obtained by extracting a nettle mist water mist with water, a hydrophilic organic solvent or a mixed solvent thereof. 4. A hyaluronidase inhibitor comprising a hyaluronidase activity inhibitory substance obtained as an active ingredient, which is obtained by extracting the nettle mist water fodder with water, a hydrophilic organic solvent or a mixed solvent thereof. 5. The collagen production promoter and active oxygen scavenger according to claim 1, wherein the hydrophilic organic solvent is selected from methanol, ethanol, propanol, 1,3-butylene glycol, glycerin and propylene glycol. Agents, radical scavengers, or hyaluronidase inhibitors. 6. A skin cosmetic containing an active ingredient extracted from the nettle mist water fodder according to any one of claims 1 to 5. 7. Ascorbic acid and its derivatives,
The skin cosmetic according to claim 6, wherein one or more skin lightening agents selected from the group consisting of placenta extract, kojic acid, rucinol, arbutin, ellagic acid and chamomile extract are mixed. 8. A cosmetic food or drink containing an active ingredient extracted from the nettle mist water fodder according to any one of claims 1 to 5.