JP2003225083A - Disc-shaped culturing medium - Google Patents
Disc-shaped culturing mediumInfo
- Publication number
- JP2003225083A JP2003225083A JP2002028141A JP2002028141A JP2003225083A JP 2003225083 A JP2003225083 A JP 2003225083A JP 2002028141 A JP2002028141 A JP 2002028141A JP 2002028141 A JP2002028141 A JP 2002028141A JP 2003225083 A JP2003225083 A JP 2003225083A
- Authority
- JP
- Japan
- Prior art keywords
- disc
- substrate
- medium
- culture medium
- shaped
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
【0001】[0001]
【発明の属する技術分野】本発明は、菌などの生物体の
培養技術に関する。詳しくは、均一な厚みの薄層培地を
内層部に備える層構造の円盤状培養媒体と該培養媒体を
用いた培養手法及び前記円盤状培養媒体の製造方法に関
する。TECHNICAL FIELD The present invention relates to a technique for culturing organisms such as bacteria. More specifically, the present invention relates to a disc-shaped culture medium having a layered structure in which a thin layer medium having a uniform thickness is provided in an inner layer portion, a culture method using the culture medium, and a method for producing the disc-shaped culture medium.
【0002】[0002]
【従来の技術】従来から、所定の培地が収容されたシャ
ーレやフラスコ等の培養器具を用いて、細胞、組織、器
官、微生物・菌類等の生物体を培養することが一般的に
行われている。2. Description of the Related Art Conventionally, it has been common practice to culture cells, tissues, organs, organisms such as microorganisms and fungi using a culture instrument such as a petri dish or a flask containing a predetermined medium. There is.
【0003】添付した図9には、固形培地を用いる細菌
の培養技術の一般的な手順が簡略に示されている。ま
ず、滅菌室においてシャーレ100を所定の温度・時間
条件で滅菌するとともに、別途、培地となる寒天をコル
ベン等の容器101に入れて溶解し、オートクレーブで
加温加圧して殺菌する。次に、滅菌されたシャーレ10
0に対して液状の培地102aを、均一な厚みになるよ
うに、かつ泡が混入しないように、慎重に流し入れ、固
化させる。続いて、白金耳103を滅菌し、培養対象の
菌を白金耳103の先で掬い取り、固化された培地10
2bの上に塗布し、所定の温度・時間条件で培養する。
培地102bに菌が増殖していれば、コロニーが形成さ
れる。これを光学顕微鏡104で観察する場合には、前
記培地102bを所定の大きさに切り取ってプレパラー
トガラス105上に観察試料を作成し、その上からカバ
ーガラス107を載置して観察を行う。FIG. 9 attached herewith outlines a general procedure of a bacterial culture technique using a solid medium. First, the petri dish 100 is sterilized in a sterilization chamber under predetermined temperature and time conditions, and agar serving as a medium is separately placed in a container 101 such as Kolben to dissolve it, and sterilized by heating under pressure in an autoclave. Next, the sterilized petri dish 10
The liquid medium 102a, which is liquid with respect to 0, is carefully poured and solidified so as to have a uniform thickness and not to mix bubbles. Subsequently, the platinum loop 103 is sterilized, and the bacteria to be cultured are scooped with the tip of the platinum loop 103 to solidify the medium 10.
It is applied on 2b and cultured at a predetermined temperature and time.
If bacteria are growing in the medium 102b, colonies are formed. When observing this with the optical microscope 104, the culture medium 102b is cut into a predetermined size to prepare an observation sample on the slide glass 105, and the cover glass 107 is placed on the observation sample to perform observation.
【0004】[0004]
【発明が解決しようとする課題】しかしながら、上記し
た従来の培養技術では、主に次のような技術的課題があ
った。However, the above-mentioned conventional culture technique has the following technical problems.
【0005】(1)使用するシャーレを毎回殺菌する必
要があった。(2)シャーレ上に均一の厚みの培地を作
製することが難しかった。(3)培地上に白金耳を用い
て菌を均一に塗布できるようになるには熟練を要した。
(4)複数の培養系をつくる場合は、別個独立のシャー
レを用いて個々に上記培養手順を繰り返す必要があり手
間がかかっていた。(5)培養された菌の観察作業は、
培地の切り取り作業やプレパラートとカバーガラスを用
いた所定の準備作業が必要であるので面倒であった。(1) It was necessary to sterilize the petri dish used each time. (2) It was difficult to prepare a medium having a uniform thickness on a petri dish. (3) It took skill to be able to apply the bacteria uniformly on the medium using the platinum loop.
(4) When preparing a plurality of culture systems, it was necessary to repeat the culture procedure individually using separate Petri dishes, which was troublesome. (5) The observation work of the cultured bacteria is
It was troublesome because the work of cutting out the medium and the predetermined preparation work using the slide and the cover glass were required.
【0006】そこで、本発明の主な目的は、菌等の生物
体の培養作業を飛躍的に簡易化できる培養技術を提供す
ることにある。Therefore, a main object of the present invention is to provide a culture technique capable of dramatically simplifying the work of culturing organisms such as bacteria.
【0007】[0007]
【課題を解決するための手段】上記技術的課題を解決す
るために、まず、本発明では、基板と、該基板上に設け
られた培地層と、該培地層を覆うカバーシートと、から
なる層構造を少なくとも備える円盤状培養媒体を提供す
る。In order to solve the above technical problems, first of all, the present invention comprises a substrate, a medium layer provided on the substrate, and a cover sheet covering the medium layer. Provided is a disc-shaped culture medium having at least a layered structure.
【0008】即ち、前記円盤状培養媒体は、細胞、組
織、器官、微生物・菌類等の生物体を培養するための培
地層と、この培地層を直接又は間接に保持する機能を備
える合成樹脂等で形成された基板と、前記培地層を被覆
する目的のカバーシートと、から構成される層構造部分
を少なくとも備えていることを特徴とする外観円盤状
(ディスク状)の培養媒体である。この培養媒体は、そ
の製造過程から滅菌状態で得られてくるので、滅菌処理
をせずにそのまま使用できるという利点を有する。That is, the disc-shaped culture medium is a medium layer for culturing organisms such as cells, tissues, organs, microorganisms and fungi, and a synthetic resin having a function of directly or indirectly holding the medium layer. A disc-shaped culture medium having an outer appearance, comprising at least a layered structure portion comprising a substrate formed in (1) and a cover sheet for covering the medium layer. Since this culture medium is obtained in a sterilized state during the manufacturing process, it has the advantage that it can be used as it is without sterilization.
【0009】中間に介装されている培地層は、基板の上
面に溶液状態で塗布可能であって、塗布後には固化可能
な性質を備える固形性培地からなる。この培地層には、
具体的形態を問わず、外部から培養液を送り込むことが
できるように工夫する。なお、この培地層の厚みは、培
養対象となる生物体、必要な培地量等の条件によって適
宜選択する。また、カバーシートは、100μm程度の
薄厚のポリカーボネート製シートを採用することが望ま
しいが、該カバーシートの厚み、材料は、目的に応じて
適宜選択できる。The medium layer interposed in the middle comprises a solid medium which can be applied in a solution state on the upper surface of the substrate and can be solidified after application. In this medium layer,
Regardless of the specific form, devise so that the culture solution can be fed from the outside. The thickness of the medium layer is appropriately selected according to the conditions such as the organism to be cultured and the required amount of medium. Further, it is desirable to use a polycarbonate sheet having a thin thickness of about 100 μm as the cover sheet, but the thickness and material of the cover sheet can be appropriately selected according to the purpose.
【0010】この円盤状培養媒体は、基板とカバーシー
トの間に、培地層が予め所定の厚みに規定されて介装さ
れているので、一連の培養作業において、従来のような
手間のかかる培地作製作業が不要となるという大きな利
点を有する。In this disc-shaped culture medium, since the medium layer is preliminarily set to have a predetermined thickness between the substrate and the cover sheet, the culture medium is laborious as in the conventional case in a series of culture operations. It has a great advantage that the manufacturing work becomes unnecessary.
【0011】ここで、本発明に係る円盤状培養媒体にお
いては、前記基板と前記カバーシートが、固化された前
記培地層を介して貼り合わされた構成を採用できる。即
ち、基板表面に塗布された液状の培地層の固化作用を利
用し、カバーシートを培地層表面に直接、接着固定する
ことができる。この手段によって、本発明に係る円盤状
培養媒体を、基板と培地層とカバーシートからなる単純
三層構造とすることができる。Here, in the disc-shaped culture medium according to the present invention, a structure in which the substrate and the cover sheet are bonded together via the solidified medium layer can be adopted. That is, the cover sheet can be directly adhered and fixed to the surface of the medium layer by utilizing the solidifying action of the liquid medium layer applied on the surface of the substrate. By this means, the disc-shaped culture medium according to the present invention can have a simple three-layer structure composed of a substrate, a medium layer and a cover sheet.
【0012】また、本発明に係る円盤状培養媒体では、
カバーシートを少なくとも可視光領域において光透過性
を備える合成樹脂によって形成することができる。この
手段によって、培地層の培養状態を、カバーシートを通
じて直接、目視観察又は顕微鏡観察を行うことができる
ようになる。とりわけ顕微鏡観察では、本発明に係る円
盤状培養媒体自体をそのまま顕微鏡のプレパラート台に
設置して観察できるので、従来のようなプレパラートを
用いた観察用試料の作製作業が不要となる。Further, in the disc-shaped culture medium according to the present invention,
The cover sheet can be formed of a synthetic resin having a light transmitting property at least in the visible light region. By this means, the culture state of the medium layer can be directly observed through a cover sheet or visually observed through a microscope. In particular, in microscopic observation, the disc-shaped culture medium according to the present invention itself can be directly installed on a preparation stand of a microscope for observation, and thus the conventional preparation work of an observation sample using a preparation is unnecessary.
【0013】ここで、本発明に係る円盤状培養媒体に
は、該培養媒体の直径方向中心部に凹設され、培養液が
貯留される液溜部と、該液溜部に開口して、前記培地層
に延びる内部空洞の条溝と、を設けるように工夫し、更
には、この条溝を、上方視、放射状に配設する。液溜部
の容量及びサイズは、主に培地層に対する培養液の必要
量に応じて適宜選択する。また、前記条溝の数も、各条
溝の周辺に広がるコロニー同士が接しない程度の密度本
数で、必要に応じて適宜選択してよい。なお、本願明細
書において「条溝」とは、その幅や深さを問わず、筋条
に延びる凹溝を意味し、「培養液」とは、培養対象の菌
等の生物体を含有するように調整された溶液を意味す
る。Here, in the disc-shaped culture medium according to the present invention, a liquid reservoir portion which is recessed in the central portion in the diameter direction of the culture medium and stores the culture fluid, and which is opened in the liquid reservoir portion, It is devised so as to provide a groove of an internal cavity extending to the medium layer, and further, the groove is arranged radially when viewed from above. The volume and size of the liquid reservoir are appropriately selected mainly depending on the required amount of the culture solution for the medium layer. Further, the number of the grooves is also a density number such that colonies spreading around each groove do not come into contact with each other, and may be appropriately selected as necessary. In the specification of the present application, the "groove" means a groove extending in the streak regardless of its width or depth, and the "culture liquid" contains an organism such as a bacterium to be cultured. Means a solution prepared as above.
【0014】上記した液溜部と条溝を設けた構成では、
前記液溜部に所定容量の培養液を注入し、該培養液を液
溜部に開口する各条溝へ毛細管現象を利用して所定量を
送液することが可能となる。この結果、菌培養の場合、
各条溝にほぼ沿うようにコロニーが形成され、全体とし
ては、略放射状のコロニーを形成することができる。な
お、前記液溜部を複数の部屋に区切り、各部屋に対して
種類の異なる培養液を注入することにより、一枚の円盤
状培養媒体において複数の培養系を形成できるので、培
養目的によっては大変便利である。In the structure having the above-mentioned liquid reservoir and the groove,
It is possible to inject a predetermined volume of the culture solution into the liquid reservoir and to deliver a predetermined amount of the culture solution to each groove formed in the liquid reservoir by utilizing the capillary phenomenon. As a result, in the case of bacterial culture,
A colony is formed so as to extend substantially along each groove, and a substantially radial colony can be formed as a whole. By dividing the liquid reservoir into a plurality of chambers and injecting different kinds of culture liquids into the respective chambers, a plurality of culture systems can be formed in one disc-shaped culture medium, and therefore, depending on the culture purpose. It's very convenient.
【0015】ここで、前記条溝にも培地層を形成するこ
とによって、条溝内での培養が確実に行うことができ
る。これにより、条溝に沿う、一層直線状のコロニーが
得られ易くなる。By forming a medium layer in the groove, the culture in the groove can be surely performed. This makes it easier to obtain more linear colonies along the groove.
【0016】また、前記液溜部には、前記培養液の逆流
を防止するための封止弁が装着できるように工夫するこ
とによって、毛細管現象を利用した各条溝への培養液の
送液作業が、より円滑に行うことができる。Further, the liquid reservoir is devised so that a sealing valve for preventing the backflow of the culture solution can be attached, so that the culture solution is sent to each groove utilizing the capillary phenomenon. Work can be performed more smoothly.
【0017】即ち、このような液溜部と条溝とを備える
円盤状培養媒体によれば、上記構成の液溜部に培養液を
注入する培養液注入手順と、上記構成の条溝に対し、毛
細管現象により前記培養液を送り込む送液手順と、を備
える簡易な培養液導入方法を提供できる。そして、前記
送液手順を、前記円盤状培養媒体を回転させながら行う
ことによって、前記回転に伴う遠心力により、培養液を
条溝の外周側末端部にまで確実に送り込むことが可能と
なる。また、上記円盤状培養媒体を用いた培養では、こ
の円盤状培養媒体を直接観察することを特徴とする培養
状態確認方法も提供できる。That is, according to the disk-shaped culture medium having such a liquid reservoir and the groove, the culture liquid injection procedure for injecting the culture liquid into the liquid reservoir having the above-mentioned structure and the groove having the above-mentioned structure It is possible to provide a simple culture medium introduction method including a liquid feeding procedure for feeding the culture medium by the capillary phenomenon. Then, by performing the liquid feeding procedure while rotating the disc-shaped culture medium, it becomes possible to reliably feed the culture liquid to the outer peripheral side end portion of the groove by the centrifugal force accompanying the rotation. Further, in the culture using the above disc-shaped culture medium, a culture state confirmation method characterized by directly observing the disc-shaped culture medium can be provided.
【0018】以上説明した本発明に係る円盤状培養媒体
は、次の(a)〜(d)の工程により製造することがで
きる。即ち、(a)合成樹脂を射出成形し、上方視放射
状に配設された条溝を備える円盤状の基板を形成する工
程、(b)前記基板に培地を塗布する工程、(c)塗布
された前記培地に円盤状のカバーシートを載置する工
程、(d)前記培地を固化して前記基板と前記カバーシ
ートを貼り合わせる工程、によって本発明に係る円盤状
培養媒体を製造することができる。より好適には、前記
(b)工程において、スピンコータを用いて培地を基板
に塗布する。The disc-shaped culture medium according to the present invention described above can be manufactured by the following steps (a) to (d). That is, (a) a step of injection-molding a synthetic resin to form a disk-shaped substrate having grooves arranged radially from above, (b) a step of applying a medium to the substrate, (c) an application The disc-shaped culture medium according to the present invention can be produced by the step of placing a disc-shaped cover sheet on the medium, and (d) the step of solidifying the medium and bonding the substrate and the cover sheet together. . More preferably, in the step (b), the medium is applied to the substrate using a spin coater.
【0019】なお、「スピンコータ」とは、塗布材料
(本発明では、液状培地)を、均等量で連続吐出する手
段と、円盤状の塗布対象物(本発明では、基板)を所定
速度で回転させる手段と、を少なくとも備える塗布用装
置を意味し、一般に、光ディスク等の記録媒体の貼り合
わせ工程において使用されることが多い。The "spin coater" means means for continuously discharging the coating material (liquid medium in the present invention) in an equal amount, and a disc-shaped object to be coated (substrate in the present invention) rotating at a predetermined speed. And a means for causing the coating means to be provided at least, and is generally used in the step of attaching recording media such as optical disks.
【0020】前記製造工程について、より具体的に説明
する。まず、(a)工程では、高温条件で合成樹脂を射
出成形して基板を製造するので、該製造工程からは滅菌
された状態の基板が得られるという利点がある。(b)
工程では、前記(a)工程から得られる滅菌状態の基板
の上面に、所定の栄養源等を含む液状の培地を吐出(又
は滴下)しながら、均一な厚みになるように塗布する。
好適には、前記スピンコータを用いて、気泡の混入を確
実に防ぎながら、基板上面に均一な厚みの培地を薄く塗
布する。この(b)工程では、塗布された培地に気泡の
混入がないこと、該培地が均一な厚みであることが特に
重要である。前記(c)工程では、基板上に塗布された
培地上面に、薄厚のカバーシートを載置する。この際、
培地上面に空気を残さない載置手段が好適である。前記
(d)工程では、液状の培地を固形培地化することによ
って、培地層を介して、カバーシートと基板を貼り合わ
せる。The manufacturing process will be described more specifically. First, in the step (a), a synthetic resin is injection-molded under high temperature conditions to manufacture a substrate, so that there is an advantage that a sterilized substrate can be obtained from the manufacturing process. (B)
In the step, a liquid culture medium containing a predetermined nutrient source or the like is discharged (or dropped) onto the upper surface of the sterilized substrate obtained in the step (a), and applied so as to have a uniform thickness.
Preferably, the spin coater is used to thinly apply a medium having a uniform thickness to the upper surface of the substrate while surely preventing the inclusion of air bubbles. In this step (b), it is particularly important that air bubbles are not mixed in the applied medium and that the medium has a uniform thickness. In the step (c), a thin cover sheet is placed on the upper surface of the culture medium applied on the substrate. On this occasion,
A mounting means that does not leave air on the upper surface of the medium is suitable. In the step (d), the cover medium and the substrate are attached to each other via the medium layer by converting the liquid medium into a solid medium.
【0021】これら(a)〜(d)の工程によって、上
層から順に、カバーシート、培地層、基板が積層された
層構造を備える円盤状培養媒体を製造することができ
る。By these steps (a) to (d), it is possible to manufacture a disk-shaped culture medium having a layered structure in which a cover sheet, a medium layer and a substrate are laminated in this order from the upper layer.
【0022】以上のように、本発明は、薄厚の固形培地
層が介装された層構造を備える新形態の培養ツール、そ
してこの培養ツールの好適な製造方法並びに使用方法
(培養液導入方法、培養状態確認方法)を関連産業界に
提供するという技術的意義を有している。As described above, the present invention provides a new-type culture tool having a layered structure in which a thin solid medium layer is interposed, and a suitable method for producing and using this culture tool (method for introducing a culture solution, It has the technical significance of providing a culture state confirmation method) to related industries.
【0023】[0023]
【発明の実施の形態】以下、添付図面に基づき、本発明
の好適な実施形態について説明する。Preferred embodiments of the present invention will be described below with reference to the accompanying drawings.
【0024】図1は、本発明に係る円盤状培養媒体の外
観斜視図、図2(A)及び図2(B)は、図1に示す符
号Xに示す部分の縦断面図、図3は、同円盤状培養媒体
を構成する基板を上方から視た平面図である。FIG. 1 is an external perspective view of a disc-shaped culture medium according to the present invention, FIGS. 2A and 2B are longitudinal sectional views of a portion indicated by reference numeral X in FIG. 1, and FIG. FIG. 3 is a plan view of a substrate constituting the same disk-shaped culture medium as viewed from above.
【0025】図1等に符号1で示されている円盤状培養
媒体(以下、単に「培養媒体」と称する。)は、数ミリ
程度の薄厚の円盤状(ディスク状)の形態を備える。そ
の最下層には、基板2が設けられ、該基板2の上面には
極細の条溝21が複数形成されているとともに、直径方
向中心部には、上方視円形の液溜部22が凹設されてい
る(主に図3参照)。The disc-shaped culture medium indicated by reference numeral 1 in FIG. 1 and the like (hereinafter, simply referred to as “culture medium”) has a disc-shaped (disk-shaped) form with a thickness of about several millimeters. A substrate 2 is provided in the lowermost layer thereof, and a plurality of fine grooves 21 are formed on the upper surface of the substrate 2, and a circular liquid reservoir portion 22 is formed in a concave shape at a central portion in the diametrical direction. (Mainly refer to FIG. 3).
【0026】該基板2の条溝21を含む上面には、固形
の培地層3aが均等な厚みで設けられ、最上層に固定配
置される光透過性のカバーシート4からは前記培地層3
aが透けて見える構成となる。なお、図1等では、説明
の便宜上、条溝21のサイズを誇張して表している。On the upper surface including the groove 21 of the substrate 2, a solid medium layer 3a is provided with a uniform thickness, and the medium layer 3 is fixed from the light-transmitting cover sheet 4 fixedly arranged on the uppermost layer.
The structure a is transparent. Note that in FIG. 1 and the like, the size of the groove 21 is exaggerated for convenience of description.
【0027】更に、図2に基づき、培養媒体1の構成に
ついて詳説すると、培養媒体1は、ポリカーボネート、
アクリル樹脂等の合成樹脂から形成された基板2と、こ
の基板2上に積層された固形状態の培地層3aと、更
に、この培地層3a上に積層された光透過性のカバーシ
ート4と、から構成された層構造を少なくとも備えてい
る。Further, the structure of the culture medium 1 will be described in detail with reference to FIG.
A substrate 2 formed of a synthetic resin such as an acrylic resin, a solid-state medium layer 3a laminated on the substrate 2, and a light-transmitting cover sheet 4 laminated on the medium layer 3a; At least a layered structure composed of
【0028】なお、前記層構造は、少なくとも、基板
2、培地層3a、カバーシート4の各層を備えることが
必須構成であって、それ以外の中間層(例えば、基板2
表面に形成され得るコーティング層等)の存在を排除す
る趣旨ではない。It is essential that the layer structure includes at least the substrate 2, the medium layer 3a, and the cover sheet 4, and the other intermediate layers (for example, the substrate 2).
It is not intended to exclude the presence of a coating layer that may be formed on the surface).
【0029】基板2は、本実施形態においては、直径8
cm、厚み1.2mmとされ、合成樹脂を射出成形して
得る。この基板2のサイズは、前記サイズに限定され
ず、用途に応じて適宜選択できることは言うまでもな
い。The substrate 2 has a diameter of 8 in this embodiment.
cm, thickness 1.2 mm, obtained by injection molding synthetic resin. Needless to say, the size of the substrate 2 is not limited to the above size and can be appropriately selected according to the application.
【0030】この基板2の上面2aには、基板2の中心
部の液溜部22から外周側へ筋状に延びて、上方視放射
状に、円周方向等間隔で配設された条溝21が、計8本
配設されている(図1、図3参照)。この条溝21の本
数は、用途に応じて適宜選択でき、8本に限定されな
い。On the upper surface 2a of the substrate 2, there are linear grooves 21 extending in a streak pattern from the liquid reservoir 22 at the center of the substrate 2 toward the outer peripheral side and arranged radially at an equal interval in the circumferential direction. However, a total of eight are provided (see FIGS. 1 and 3). The number of the groove 21 can be appropriately selected according to the application and is not limited to eight.
【0031】図3に基づいて、条溝21の構成を更に詳
説すると、各条溝21は、直径10mm、深さ1mmの
サイズに形成された前記液溜部22の内周壁面22aに
開口部21aを備え、基板2の外周壁23に近接する位
置又は末端にまで形成されている。この条溝21は、液
溜部22に注入されることになる液状の培地を、毛細管
現象を利用して外周方向に送液できるキャピラリー機能
を発揮でき、かつ所望の生物体を培養できる程度のサイ
ズに形成すれば良い。例えば、幅は1μm以上、深さ1
μm以上に形成する。The structure of the groove 21 will be described in more detail with reference to FIG. 3. Each groove 21 has an opening formed in the inner peripheral wall surface 22a of the liquid reservoir 22 formed to have a diameter of 10 mm and a depth of 1 mm. 21a and is formed at a position close to the outer peripheral wall 23 of the substrate 2 or at the end. The groove 21 has a capillary function capable of delivering the liquid medium to be injected into the liquid reservoir 22 in the outer peripheral direction by utilizing the capillary phenomenon, and is capable of culturing a desired organism. It can be formed into a size. For example, the width is 1 μm or more and the depth is 1
It is formed to a thickness of μm or more.
【0032】なお、この条溝21は、基板2に凹設され
た、図2(A)に示される実施例形態だけでなく、図2
(B)に示されたような、カバーシート4側に凹設する
変形形態を採用することもできる。カバーシート4側に
条溝21を形成する場合でも、該条溝21内壁面に沿う
培地層3aを適宜の方法で形成してよい。The groove 21 is not limited to the embodiment shown in FIG.
It is also possible to employ a modified form in which the cover sheet 4 is recessed as shown in (B). Even when the groove 21 is formed on the cover sheet 4 side, the medium layer 3a along the inner wall surface of the groove 21 may be formed by an appropriate method.
【0033】図4は、本発明に係る培養媒体1を構成す
るカバーシートを上方から視た平面図である。FIG. 4 is a plan view of the cover sheet constituting the culture medium 1 according to the present invention, as viewed from above.
【0034】カバーシート4は、上方視した時の外観が
略ドーナッツ状又はコンパクトディスク(CD)状の形
態を備える、光透過性の薄厚シートである。このカバー
シート4は、少なくとも可視光領域での光透過性を有す
る合成樹脂によって成形されており、その好適な厚み
は、100μm程度である。The cover sheet 4 is a light-transmissive thin sheet having a substantially donut shape or a compact disc (CD) shape when viewed from above. The cover sheet 4 is formed of a synthetic resin having a light transmitting property at least in the visible light region, and its preferable thickness is about 100 μm.
【0035】このカバーシート4は、培地層3を被覆
し、保護する役割を果たす。なお、このカバーシート4
のガス透過性を制御することによって、通性、好気性、
嫌気性のいずれの培養条件を自在に作り出せるので、培
養媒体1は、培養器具としての汎用性を備えている。The cover sheet 4 serves to cover and protect the medium layer 3. In addition, this cover sheet 4
By controlling the gas permeability of
Since any anaerobic culture condition can be freely created, the culture medium 1 has versatility as a culture tool.
【0036】また、カバーシート4は、光透過性である
ため、培地層3に増殖した生物体やコロニーを外から観
察することができる。図4に示す符号41は、カバーシ
ート4の中心部に形成された円孔であって、カバーシー
ト4は、該円孔41が基板2の液溜部22上部に正確に
重なる様に位置決めされて、培地層3上に積層されるこ
とになる(図1参照)。Since the cover sheet 4 is light-transmissive, organisms and colonies grown on the medium layer 3 can be observed from the outside. Reference numeral 41 shown in FIG. 4 is a circular hole formed in the central portion of the cover sheet 4, and the cover sheet 4 is positioned so that the circular hole 41 exactly overlaps the upper part of the liquid reservoir 22 of the substrate 2. Then, they are stacked on the medium layer 3 (see FIG. 1).
【0037】次に図5は、培養媒体1の製造方法の製造
工程を示す図である。本発明に係る培養媒体1の製造方
法は、図5に示されたP1〜P4で示された工程を少な
くとも備えていることを特徴とする。以下、各工程P1
〜P4について順に説明する。Next, FIG. 5 is a diagram showing manufacturing steps of the method for manufacturing the culture medium 1. The method for producing the culture medium 1 according to the present invention is characterized by including at least the steps shown by P 1 to P 4 shown in FIG. Hereinafter, each step P 1
~ P 4 will be described in order.
【0038】<工程P1について>該工程P1は、合成
樹脂を射出成形して、上方視放射状に配設された条溝2
1を備える円盤状の基板2を一体成形する工程である。
前記射出成形の具体的方法は特に限定されないが、この
射出成形は、樹脂温度約300℃、金型温度約120℃
の条件で行われるため、成形される基板2は滅菌された
状態で得られてくる。この結果、基板2、生物体培養用
の培養媒体1を構成する好適な部材となる。<Regarding Step P 1 > In the step P 1 , a synthetic resin is injection-molded, and the grooves 2 are arranged radially in a top view.
It is a step of integrally molding the disk-shaped substrate 2 provided with 1.
Although the specific method of the injection molding is not particularly limited, the injection molding is performed at a resin temperature of about 300 ° C. and a mold temperature of about 120 ° C.
The substrate 2 to be molded is obtained in a sterilized state since the above process is performed. As a result, the substrate 2 and the culture medium 1 for culturing organisms are suitable members.
【0039】<工程P2について>該工程P2は、前記
工程P1から得られる前記基板2に対して、液状の培地
3aを塗布する工程である。具体的には、まず、基板2
を回転体5aに保持させ、該回転体5aの駆動によって
基板2を回転させながら(矢印Y参照)、該基板2の上
方所定位置に配置されたノズル5bから液状の培地3b
を吐出する。<Regarding Process P 2 > The process P 2 is a process of applying the liquid medium 3a to the substrate 2 obtained from the process P 1 . Specifically, first, the substrate 2
Is held by the rotating body 5a, and while the substrate 2 is rotated by the driving of the rotating body 5a (see arrow Y), the liquid medium 3b is discharged from the nozzle 5b arranged at a predetermined position above the substrate 2.
Is discharged.
【0040】即ち、回転体5aとノズル5bと少なくと
も備える構成のいわゆるスピンコータ装置を用いて、基
板2の上面に、前記培地3aを均等な厚み(例えば、1
00μm)で塗布する。塗布する培地3aの配合構成
は、培養目的に応じて適宜選択可能であって、少なくと
も固形培地(半固形培地も含む。)となるものであれば
よく、更にはスピンコータによる塗布作業に好適なもの
であればよいThat is, using a so-called spin coater device having at least the rotating body 5a and the nozzle 5b, the culture medium 3a is provided on the upper surface of the substrate 2 with a uniform thickness (for example, 1).
00 μm). The composition of the medium 3a to be applied can be appropriately selected according to the purpose of culturing and may be at least a solid medium (including a semi-solid medium), and further suitable for application by a spin coater. If
【0041】<工程P3について>該工程P3は、塗布
された前記培地3aの上面に、円盤状のカバーシート4
を載置する工程である。具体的には、カバーシート4を
エア吸引パッド等で保持して、基板2の上方位置まで移
送した後、保持状態を解除し、基板2に塗布された培地
3aの上面に、カバーシート4を載置する。この際、カ
バーシート4と培地3aとの間に、気泡が残存又は混入
しないようにするために、カバーシート4を上方側に反
らせるように保持し、この反りの戻り作用を利用して空
気を外周側に追い出しながら載置するようにしてもよ
い。<Regarding Step P 3 > In the step P 3 , the disc-shaped cover sheet 4 is formed on the upper surface of the applied culture medium 3 a.
Is the step of placing. Specifically, after holding the cover sheet 4 with an air suction pad or the like and transferring it to a position above the substrate 2, the holding state is released, and the cover sheet 4 is placed on the upper surface of the medium 3a applied to the substrate 2. Place it. At this time, in order to prevent air bubbles from remaining or mixing between the cover sheet 4 and the culture medium 3a, the cover sheet 4 is held so as to be warped upward and air is utilized by utilizing the returning action of the warp. You may make it mount, pushing out to the outer peripheral side.
【0042】ここで、カバーシート4を基板2の上面に
塗布された液状の培地3aに載置するときに、表面張力
によって条溝21の壁面に沿って形成された培地3a
(図2参照)が上方へ引き上げられ、条溝21の空洞部
S(図2参照)を閉塞してしまうおそれがある。空洞部
Sが閉塞されると、後述する培養液を条溝21に送液で
きなくなるという問題が起きる。Here, when the cover sheet 4 is placed on the liquid medium 3a applied to the upper surface of the substrate 2, the medium 3a formed along the wall surface of the groove 21 by the surface tension.
(See FIG. 2) may be pulled upward, and the cavity S (see FIG. 2) of the groove 21 may be closed. When the cavity S is closed, there arises a problem that a culture solution described later cannot be sent to the groove 21.
【0043】この問題の発生を防止するための手法とし
て、条溝21の形状を適切に選定するという手法が考え
られる。即ち、条溝21を深く形成することによって、
培地3aを条溝21内に留めることができる。一例とし
て、条溝21を、アスペクト比(溝の幅Wと高さHの
比:H/W、図2参照)が1.0以上になるように形成
することによって、前記問題の発生を有効に防止でき
る。なお、培地3aの種類に応じて、適切なアスペクト
比を選択してもよい。As a method for preventing the occurrence of this problem, a method of appropriately selecting the shape of the groove 21 can be considered. That is, by forming the groove 21 deep,
The medium 3a can be retained in the groove 21. As an example, by forming the groove 21 so that the aspect ratio (ratio of the width W of the groove to the height H: H / W, see FIG. 2) is 1.0 or more, the occurrence of the above problem is effective. Can be prevented. An appropriate aspect ratio may be selected according to the type of medium 3a.
【0044】<工程P4について>該工程P4は、基板
2に塗布された液状の培地3aを固化させることによっ
て、前記基板2と前記カバーシート4を貼り合わせる工
程である。具体的には、図示しないオートクレーブで加
圧加温を行い、その後、培地3aが固化するまで室温に
静置することによって、基板2とカバーシート4の間に
固形の培地層3aを形成しながら、カバーシート4を培
地層3に接着させる。即ち、培地層3を介して、基板2
とカバーシート4を貼り合わせる。このようにして、本
発明に係る培養媒体1を確実に製造することができる。<Regarding Process P 4 > The process P 4 is a process of bonding the substrate 2 and the cover sheet 4 by solidifying the liquid medium 3 a applied to the substrate 2. Specifically, by heating under pressure in an autoclave (not shown) and then leaving it at room temperature until the medium 3a is solidified, a solid medium layer 3a is formed between the substrate 2 and the cover sheet 4. The cover sheet 4 is adhered to the medium layer 3. That is, through the medium layer 3, the substrate 2
And the cover sheet 4 are pasted together. In this way, the culture medium 1 according to the present invention can be reliably manufactured.
【0045】図6は、上記製造工程P(P1〜P4)に
よって形成された本発明に係る培養媒体1の液溜部22
に装着される封止弁5の構成を説明するための一部断面
の部分拡大図、図7は、培養液7を液溜部22に注入す
る様子を示す図、図8は、液溜部22に注入された培養
液7が条溝21を外周方向に進行する様子を示す図、で
ある。FIG. 6 shows a liquid reservoir 22 of the culture medium 1 according to the present invention formed by the above-mentioned manufacturing process P (P 1 to P 4 ).
FIG. 7 is a partially enlarged view of a partial cross section for explaining the configuration of the sealing valve 5 attached to the liquid storage device, FIG. 7 is a view showing a state of injecting the culture solution 7 into the liquid storage part 22, and FIG. It is a figure which shows the mode that the culture solution 7 inject | poured into 22 progressed in the groove 21 in the outer peripheral direction.
【0046】前記図6〜図8に基づいて、培養媒体1に
対する培養液導入方法について説明する。まず、基板2
の中心部の液溜部22は、培養目的の菌等を含む培養液
7が注入され(図7参照)、また、この培養液7が一旦
貯留される箇所として機能する。A method for introducing the culture solution into the culture medium 1 will be described with reference to FIGS. 6 to 8. First, the substrate 2
The liquid reservoir 22 in the central portion of (1) functions as a place where the culture liquid 7 containing the bacteria for culturing is injected (see FIG. 7) and the culture liquid 7 is temporarily stored.
【0047】なお、図6に示す符号25は、基板2の下
面中心部に形成された凹部であって、該凹部25は、上
記したスピンコータを構成する回転体5aに培養媒体1
を保持する時に利用される部位である。Reference numeral 25 shown in FIG. 6 is a recess formed in the center of the lower surface of the substrate 2. The recess 25 is formed on the rotating body 5a constituting the spin coater and the culture medium 1 is formed.
It is a part used when holding.
【0048】ここで、図6に示される外観小円盤状の封
止弁5は、カバーシート4の中心部に形成されている円
孔41の内側に嵌着するように構成され、液溜部22を
密封する役割を果たす。封止弁5の固定方法は、適宜選
択可能である。Here, the external small disc-shaped sealing valve 5 shown in FIG. 6 is constructed so as to be fitted inside the circular hole 41 formed in the central portion of the cover sheet 4, and the liquid reservoir portion. It serves to seal 22. The method of fixing the sealing valve 5 can be appropriately selected.
【0049】<培養液注入手順について>密封された液
溜部22に対し、図7に示すようなインジェクター6に
よって封止弁5の中央に形成された針刺部51から、所
定の培養液7が必要量だけ注入される。前記針刺部51
は、例えば柔軟性を有するゴム素材で形成されており、
インジェクター6による穿針作業によって該インジェク
ター6の先端部を液溜部22内に導入し、インジェクタ
ー6が抜かれたときには前記素材の柔軟性により穿針部
を封止することにより、液溜部22の気密性が一定に保
持されるように構成されている。<Regarding the procedure for injecting the culture solution> With respect to the sealed liquid reservoir 22, the predetermined culture solution 7 is supplied from the needle stick portion 51 formed in the center of the sealing valve 5 by the injector 6 as shown in FIG. Is injected in the required amount. The needle stick part 51
Is formed of, for example, a flexible rubber material,
The distal end of the injector 6 is introduced into the liquid reservoir 22 by the needle puncturing operation by the injector 6, and when the injector 6 is pulled out, the flexibility of the material seals the puncture needle to prevent the liquid reservoir 22 from leaking. The airtightness is configured to be kept constant.
【0050】<送液手順について>培養液7が液溜部2
2内に注入されて充満すると、培養液7は、毛細管現象
によって、液溜部22の開口部21aから各条溝21に
流入し、基板外周側に向けて進行する(図8の矢印Z参
照)。この際、封止弁5によって液溜部22が密封され
ているので、培養液7は逆流することなく、各条溝21
へ円滑に送液されていく。<Regarding liquid feeding procedure> The culture liquid 7 is the liquid reservoir 2
When the medium 7 is filled and filled, the culture solution 7 flows into each groove 21 from the opening 21a of the liquid reservoir 22 by the capillary phenomenon and proceeds toward the outer peripheral side of the substrate (see arrow Z in FIG. 8). ). At this time, since the liquid reservoir 22 is sealed by the sealing valve 5, the culture liquid 7 does not flow backward and each groove 21
The solution is smoothly sent to.
【0051】更に、この送液手順を、図7の矢印Y、Y
に示すように、培養媒体1を水平に回転させながら行う
と、前記毛細管現象に加えて、遠心力が培養液7に付加
されるので、培養液7がより円滑に、液溜部22から各
条溝21へ送液されるようになるので、好適である。Further, this liquid feeding procedure is described by arrows Y, Y in FIG.
As shown in FIG. 4, when the culture medium 1 is horizontally rotated, a centrifugal force is added to the culture solution 7 in addition to the capillary phenomenon, so that the culture solution 7 can be more smoothly transferred from the liquid reservoir 22. It is preferable because the liquid is fed to the groove 21.
【0052】以上説明した培養媒体1を用いて所望の生
物体を培養すると、該生物体が条溝21に沿うように増
殖し、菌培養の場合にはコロニーが形成される。培養作
業が終了した後は、培養媒体1における培養状態を、目
視又は顕微鏡、CCDカメラ等を用いて、該培養媒体1
を直接観察することにより確認すること可能となる。When a desired organism is cultured using the culture medium 1 described above, the organism grows along the groove 21, and in the case of bacterial culture, colonies are formed. After the culturing work is completed, the culturing state of the culturing medium 1 is visually checked or using a microscope, a CCD camera, or the like.
It can be confirmed by directly observing.
【0053】[0053]
【発明の効果】本発明に係る円盤状培養媒体によれば、
培養に使用する器具の滅菌作業や培地形成作業が不要と
なるので、各種生物体の培養作業を飛躍的に簡易化する
ことができる。また、本発明に係る円盤状培養媒体は、
合成樹脂と培地から形成されているので、材料コストや
製造コストが安価であるので、低コストのディスポーザ
ルタイプの培養媒体を提供できる。According to the disc-shaped culture medium of the present invention,
Since sterilization work of the equipment used for culture and culture medium formation work are unnecessary, the culture work of various organisms can be dramatically simplified. Further, the disc-shaped culture medium according to the present invention,
Since it is formed of a synthetic resin and a medium, the material cost and the manufacturing cost are low, and thus a low-cost disposable type culture medium can be provided.
【0054】この円盤状培養媒体内に介装されている培
地層は、気泡の混入がなく、均等な厚みを備えているの
で、均一かつ安定した培養系を提供できる。また、この
培地層の厚みは、その製造過程において、基板への培地
の塗布量を変えるだけで自在に制御又は調整できるの
で、各種培養系に適合する培地層を備える培養媒体を提
供できるので非常に便利である。Since the medium layer interposed in the disc-shaped culture medium has no air bubbles and has a uniform thickness, a uniform and stable culture system can be provided. Further, since the thickness of the medium layer can be freely controlled or adjusted in the manufacturing process by changing the amount of the medium applied to the substrate, it is possible to provide a culture medium having a medium layer suitable for various culture systems. It is convenient for
【0055】本発明に係る円盤状培養媒体の培地層に
は、内部空洞の条溝が形成され、この条溝に対して毛細
管現象(及び遠心力)を利用して培養液を送液する構成
であるので、培地層に対する生物体の導入作業が容易化
し、初心者でも簡単にその作業を行うことができる。ま
た、本発明に係る円盤状培養媒体では、培地層の条溝に
沿うように生物体が増殖し、菌培養の場合ではコロニー
が形成されるので、生物体の増殖エリアがパターン化さ
れ、培養状態の観察を容易に行うことができる。The medium layer of the disc-shaped culture medium according to the present invention is provided with an internal hollow groove, and the culture solution is sent to the groove by utilizing the capillary phenomenon (and centrifugal force). Therefore, the work of introducing the organism into the medium layer is facilitated, and even a beginner can easily perform the work. Further, in the disc-shaped culture medium according to the present invention, the organisms grow along the grooves of the medium layer, and in the case of bacterial culture, colonies are formed, so that the growth area of the organisms is patterned and cultivated. The state can be easily observed.
【0056】本発明に係る円盤状培養媒体を用いて培養
作業を行うことにより、培地の切り取り作業やプレパラ
ート上での観察用試料作製作業が全く不要となり、該培
養媒体を顕微鏡やCCDカメラ等により直接観察するこ
とができるので大変便利であり、細菌検査等を迅速に行
うことができる。By carrying out the culturing work using the disc-shaped culturing medium according to the present invention, the work of cutting off the medium and the work of preparing the sample for observation on the preparation are completely unnecessary, and the culturing medium can be used with a microscope or a CCD camera. It is very convenient because it can be observed directly, and bacteria tests can be performed quickly.
【0057】本発明に係る円盤状培養媒体の製造方法で
は、基板と、均等な厚みの培地層と、カバーガラスと、
を備える層構造を少なくとも備える円盤状培養媒体を確
実に大量生産できる。In the method for producing a disc-shaped culture medium according to the present invention, a substrate, a medium layer having a uniform thickness, a cover glass,
It is possible to reliably mass-produce a disc-shaped culture medium including at least a layered structure including.
【0058】本発明によれば、従来のシャーレやフラス
コ等の培養器具とは全く発想を異にする層構造の培養器
具、新規な培養手法並びに培養状態確認方法を提供でき
る。According to the present invention, it is possible to provide a culture instrument having a layered structure, a novel culture method and a culture state confirmation method, which are completely different in concept from conventional culture instruments such as petri dishes and flasks.
【図1】本発明に係る円盤状培養媒体(1)の外観斜視
図FIG. 1 is an external perspective view of a disc-shaped culture medium (1) according to the present invention.
【図2】(A)図1に示す符号Xに示す部分の縦断面図 (B)同X部分の変形形態の縦断面図FIG. 2 (A) is a vertical cross-sectional view of a portion indicated by reference numeral X in FIG. (B) Vertical cross-sectional view of a modified form of the X portion
【図3】同円盤状培養媒体(1)を構成する基板(2)
を上方から視た平面図FIG. 3: Substrate (2) constituting the same disk-shaped culture medium (1)
Top view of the
【図4】同円盤状培養媒体(1)を構成するカバーシー
ト(4)を上方から視た平面図FIG. 4 is a plan view of a cover sheet (4) constituting the same disk-shaped culture medium (1) viewed from above.
【図5】同培養媒体(1)の製造方法の製造工程を示す
図FIG. 5 is a view showing manufacturing steps of the method for manufacturing the same culture medium (1).
【図6】同培養媒体(1)の液溜部(22)に装着され
る封止弁(5)の構成を説明するための一部断面の部分
拡大図FIG. 6 is a partially enlarged view of a partial cross section for explaining the configuration of the sealing valve (5) mounted on the liquid reservoir (22) of the culture medium (1).
【図7】培養液(7)を基板(2)の液溜部(22)に
注入する様子を示す図FIG. 7 is a view showing a state in which the culture solution (7) is injected into the liquid reservoir (22) of the substrate (2).
【図8】液溜部(22)に注入された培養液(7)が条
溝(21)内を外周方向に進行する様子を示す図FIG. 8 is a view showing a state in which the culture solution (7) injected into the liquid reservoir (22) proceeds in the groove (21) in the outer peripheral direction.
【図9】従来の一般的な培養工程を表す手順フロー図FIG. 9 is a procedure flow chart showing a conventional general culture process.
───────────────────────────────────────────────────── フロントページの続き Fターム(参考) 4B029 AA03 AA08 BB01 CC01 CC02 CC07 GA08 GB01 GB02 GB05 GB06 GB07 GB09 GB10 4B065 AA01X AA57X AA87X BC46 BC50 CA46 ─────────────────────────────────────────────────── ─── Continued front page F term (reference) 4B029 AA03 AA08 BB01 CC01 CC02 CC07 GA08 GB01 GB02 GB05 GB06 GB07 GB09 GB10 4B065 AA01X AA57X AA87X BC46 BC50 CA46
Claims (12)
と、該培地層を覆うカバーシートと、からなる層構造を
少なくとも備える円盤状培養媒体。1. A disc-shaped culture medium comprising at least a layer structure comprising a substrate, a medium layer provided on the substrate, and a cover sheet covering the medium layer.
れた前記培地層を介して貼り合わされた構成を備えるこ
とを特徴とする請求項1記載の円盤状培養媒体。2. The disc-shaped culture medium according to claim 1, wherein the substrate and the cover sheet have a configuration in which they are bonded together via the solidified medium layer.
領域において光透過性を備える合成樹脂により形成され
たことを特徴とする請求項1記載の円盤状培養媒体。3. The disc-shaped culture medium according to claim 1, wherein the cover sheet is made of a synthetic resin having a light transmissive property in at least a visible light region.
留される液溜部と、 該液溜部に開口し、前記基板又は前記カバーシートのい
ずれか一方に形成されている内部空洞の条溝と、 を備えることを特徴とする請求項1記載の円盤状培養媒
体。4. A liquid reservoir portion that is recessed in the central portion in the diametrical direction and stores a culture fluid, and an internal cavity that is opened in the liquid reservoir portion and is formed in either the substrate or the cover sheet. 2. The disc-shaped culture medium according to claim 1, further comprising:
止するための封止弁が装着されることを特徴とする請求
項4記載の円盤状培養媒体。5. The disc-shaped culture medium according to claim 4, wherein the liquid reservoir is equipped with a sealing valve for preventing backflow of the culture liquid.
たことを特徴とする請求項4記載の円盤状培養媒体。6. The disc-shaped culture medium according to claim 4, wherein the groove is formed radially when viewed from above.
いることを特徴とする請求項4記載の円盤状培養媒体。7. The disc-shaped culture medium according to claim 4, wherein the medium layer is formed in the groove.
と、該培地層を覆うカバーシートと、からなる層構造を
少なくとも備える円盤状培養媒体の直径方向中心部に形
成された液溜部に培養液を注入する培養液注入手順と、 前記液溜部に開口して前記培地層に延びる内部空洞の条
溝に対し、毛細管現象により前記培養液を送り込む送液
手順と、 を備える培養液導入方法。8. A liquid reservoir formed at the center in the diametrical direction of a disc-shaped culture medium, which comprises at least a layered structure comprising a substrate, a medium layer formed on the substrate, and a cover sheet covering the medium layer. Culture solution injection procedure for injecting the culture solution into the part, and a solution delivery procedure for sending the culture solution by capillary action to the groove of the internal cavity that opens in the solution reservoir and extends to the medium layer Liquid introduction method.
回転させながら行うことを特徴とする請求項8記載の培
養液導入方法。9. The culture medium introduction method according to claim 8, wherein the liquid feeding procedure is performed while rotating the disc-shaped culture medium.
と、該培地層を覆う光透過性樹脂製のカバーシートと、
からなる層構造を少なくとも備える円盤状培養媒体で生
物体を培養した後、前記円盤状培養媒体を直接観察する
ことを特徴とする培養状態確認方法。10. A substrate, a medium layer formed on the substrate, and a cover sheet made of a light-transmissive resin covering the medium layer,
A method for confirming a culture state, which comprises culturing an organism in a disc-shaped culture medium having at least a layered structure comprising, and then directly observing the disc-shaped culture medium.
も備える円盤状培養媒体の製造方法。 (a)上方視放射状に配設された条溝を備える合成樹脂
製の円盤状の基板を形成する工程。 (b)前記基板に培地を塗布する工程。 (c)塗布された前記培地に円盤状のカバーシートを載
置する工程。 (d)前記培地を固化して前記基板と前記カバーシート
を貼り合わせる工程。11. A method for producing a disc-shaped culture medium, which comprises at least the following steps (a) to (d). (A) A step of forming a disc-shaped substrate made of a synthetic resin, which is provided with stripe grooves radially arranged when viewed from above. (B) A step of applying a culture medium to the substrate. (C) A step of placing a disk-shaped cover sheet on the applied culture medium. (D) A step of solidifying the medium and bonding the substrate and the cover sheet together.
いて行うことを特徴とする請求項11記載の円盤状培養
媒体の製造方法。12. The method for producing a disk-shaped culture medium according to claim 11, wherein the step (b) is performed using a spin coater.
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US9127247B2 (en) | 2010-06-22 | 2015-09-08 | Aes Chemunex | Petri dish including locking means for forming a stack |
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