JP2003055641A - Tackifier - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a polysaccharide tackifier having high tackifying ability at a small quantity, low in viscosity change with a rise in temperature, low in viscosity change over time at a high temperature, and excellent in emulsion and dispersion stability. SOLUTION: This tackifier contains as an effective component a polysaccharide which is prepared by a method in which a polysaccharide essentially comprising monosaccharides; fucose, glucose, glucuronic acid, and rhamnose, is treated in an alkali aqueous solution having a pH of not lower than 8 at a temperature of 60-180 deg.C, then it is cooled without vigorous stirring, but keeping the rotating speed of the tip of a stirring blade within 20 m/s, or without giving a shearing force by stirring.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a microbial polysaccharide-based thickener which is used in various emulsified products and solid dispersion products and has excellent emulsification / dispersion stability and high viscosity.

[0002]

In general, emulsified products and dispersion products of fine solids have various thickening properties for the purpose of preventing separation of the emulsified system, preventing sedimentation and floating of the solid dispersion, and imparting an appropriate viscosity for easy handling. Agent is used. For example, emulsions and lotions have a low viscosity and spread well because they are applied thinly over a wide area.
Various viscosity characteristics are required depending on the purpose of use, such as that a cream or a beauty essence has an appropriate viscosity for partial application, and a pack cosmetic or a massage cosmetic needs a viscosity that does not run off. In addition, since emulsified products and dispersion products of fine solids emulsify or disperse insoluble materials such as water and various oils, higher alcohols, pigments, etc., the emulsified state or dispersed state is maintained for a long time. However, a thickener is added to prevent separation.

Thickeners used in cosmetics include synthetic polymers such as acrylic acid polymers, processed natural products such as carboxymethyl cellulose and methyl cellulose, carrageenan, guar gum, locust bean gum, gum arabic and karaya gum. Plant-derived polysaccharides,
Microorganism-derived polysaccharides such as xanthan gum are used. In particular, processed natural products and microbially produced polysaccharides have been used in many cases for the purpose of obtaining a high thickening effect with a relatively small amount. However, the polysaccharide-based thickeners produced by microorganisms have the drawback that the viscosity rapidly decreases with increasing temperature.

Therefore, a method for improving the viscous properties of the microorganism-produced polysaccharide has been proposed. For example, gellan gum which is a microorganism-derived polysaccharide and other polysaccharides are used as a method for obtaining a high viscosity by blending a thickening agent at a low concentration. A method of increasing viscosity by blending (JP-A-10-215795), or a method of heat-treating or wet-heat treating powdery xanthan gum (JP-A-10-33125, JP-A-2000-7705). ) Etc. However, a sufficiently satisfactory thickener has not yet been obtained, and higher performance is required.

[0005]

DISCLOSURE OF THE INVENTION The inventors of the present invention have improved the above-mentioned problems, provided a high viscosity increase with a small amount, and an excellent polysaccharide-type thickener without a rapid decrease in viscosity due to an increase in temperature. As a solution, a thickener containing a polysaccharide obtained by heating a polysaccharide containing fucose, glucose, glucuronic acid, and rhamnose as constituent monosaccharides at 60 to 180 ° C. in an aqueous solution having a pH of 8 or more as an active ingredient is proposed. (Patent application 2000-313
517).

However, although the flask scale production is possible, there is a problem in that the viscosity of the thickener obtained varies depending on the production lot when produced on an industrial scale as it is.

The object of the present invention is to solve the above problems and enable production on an industrial scale, and
It is to provide a thickener having excellent emulsification / dispersion stability.

[0008]

In order to solve such a problem, the inventors of the present invention have conducted diligent research on improvement of a method for producing a polysaccharide thickener on an industrial scale, and as a result, have identified a specific problem. By heat-treating saccharides in an alkaline aqueous solution and then cooling by a method that does not involve strong shearing force, it has a higher viscosity, and there is no sharp decrease in viscosity due to temperature rise, and emulsion / dispersion stability is improved. The inventors have found that an excellent polysaccharide can be obtained, and completed the present invention.

That is, the invention according to claim 1 heats a polysaccharide substantially containing fucose, glucose, glucuronic acid and rhamnose as constituent monosaccharides in an aqueous solution having a pH of 8 or more at 60 to 180 ° C. After that, a thickening containing a polysaccharide obtained as an active ingredient by a method of cooling while maintaining the tip speed of the stirring blade at a rotation speed of 2.0 m / s or less or cooling without applying shearing force by stirring. It is an agent.

In the invention according to claim 2, the molar ratio of the polysaccharide is fucose: glucose: glucuronic acid: rhamnose = 1.
The thickener according to claim 1, which is from 2: 1 to 4: 1 to 2: 1 to 2.

The invention according to claim 3 has a structure in which a polysaccharide is composed of glucose, glucuronic acid and rhamnose as a main chain, and further fucose is bonded to a side chain. Agent.

[0012] The invention according to claim 4 is the method for producing a thickening agent according to any one of claims 1 to 3, wherein the polysaccharide is a polysaccharide produced by Alcaligenes Bastus strain B-16.

According to a fifth aspect of the present invention, the method of cooling without applying shearing force by stirring is a method of passing the inside of a cooled flexible pipe under pressure, thereby increasing the viscosity. Agent.

[0014]

BEST MODE FOR CARRYING OUT THE INVENTION The present invention is described in detail below.
The thickener of the present invention has a strong shearing force after substantially heat-treating a polysaccharide containing fucose, glucose, glucuronic acid and rhamnose as constituent monosaccharides in an alkaline aqueous solution having a pH of 8 or more at 60 to 180 ° C. It is a thickener containing as an active ingredient a polysaccharide obtained by cooling by a method without. The polysaccharide that has been subjected to alkali heat treatment retains a high viscosity at high temperatures, but when it is cooled, it is not stirred or slowly stirred if it is stirred strongly from the viewpoint of enhancing cooling efficiency. Compared to the case below, the thickening effect is small and the change in viscosity with temperature is large.

The polysaccharide used in the present invention is a polysaccharide substantially containing fucose, glucose, glucuronic acid, and rhamnose as constituent monosaccharides, and preferably glucose, glucuronic acid represented by the following formula (1), It is a polysaccharide having a repeating main chain composed of rhamnose and having a structure in which one fucose is branched to one glucose.

[0016]

[Chemical 1]

The polysaccharide used in the present invention is obtained as a microbially produced polysaccharide. Generally, microorganisms
Since two or more types of polysaccharides are often produced, even if the polysaccharide used in the present invention contains other polysaccharides, other polysaccharides are included as long as the effects of the present invention are not impaired. It does not prevent you from being

Microorganisms which produce polysaccharides are not particularly limited, but for example, Alcaligenes pertus
There is a B-16 strain bacterium (FERM BP-2015).

The production of the polysaccharide of the present invention is carried out as follows, for example, in the case of Alcaligenes lettus B-16 strain bacteria.

The Alcaligenes lettus B-16 strain bacterium is cultivated by an ordinary microbial culturing method. For example, monosaccharides such as fructose, glucose and sucrose as carbon sources, natural polymers such as hemicellulose, starch and corn starch, and olives. Oils such as oils and fats, inorganic nitrogen sources such as urea, ammonium chloride, ammonium nitrate, ammonium sulfate, and organic nitrogen sources such as tryptone, yeast extract, meat extract, peptone, and malt extract are used as nitrogen sources. , Magnesium sulfate,
Using a medium to which an inorganic salt such as sodium chloride is added, aeration stirring liquid culture is performed for 3 to 10 days at an initial pH of 4 to 10 and a culture temperature of 15 to 40 ° C. After culturing, about 2 times (volume) or more of an organic solvent such as acetone, ethanol or isopropyl alcohol is added to the culture solution, and the culture product is recovered as an insoluble aggregate.

It has been confirmed that at least two kinds of polysaccharides are contained in the polysaccharide produced by Alcaligenes lettus B-16 strain bacterium, one of which is the above-mentioned formula ( A polysaccharide having a structure in which one fucose is branched to one glucose in the repeating structure consisting of glucose, glucuronic acid, and rhamnose as shown in 1), and the other one is shown in (2) below. It is a repeating polysaccharide having a structure substantially consisting of fucose and mannose as constituent monosaccharides [See 1998 Annual Meeting of the Japan Society for Agricultural Chemistry, p. 371]. Y. Nohata, J .; Azumi
a.R. Kurane, Carbohydrate R
esearch 293 , 213-222 (199)
See 6))]

[0022]

[Chemical 2]

Even if the polysaccharide represented by the above formula (2) is contained in the polysaccharide of the above formula (1), which is the polysaccharide of the present invention, its effect is not impaired. Therefore, in the above formula (2) Alkaline Genesulators B without removal of the indicated polysaccharides
Polysaccharides produced by strain -16 can be used.

The pH of the aqueous polysaccharide solution when the polysaccharide according to the present invention is subjected to the alkali heat treatment is adjusted to 8 or more, preferably 11 or more, more preferably 12 or more by adding an alkaline substance. Under acidic conditions where the pH of the aqueous solution is less than 7, the molecular chain of the polysaccharide is severely broken, and the thickening effect and the emulsifying / dispersing effect intended by the present invention may not be obtained.

The alkali heat treatment temperature is 60 ° C to 180 ° C.
℃, preferably 80-160 ℃, more preferably 100
~ 140 ° C. If it is lower than 60 ° C, the thickening effect may not be obtained even if the heating time is extended. Also, 180 ℃
If it exceeds the range, the decomposition of the polysaccharide may proceed excessively, which is not preferable. As a matter of course, in the case of mass production at 100 ° C. or higher, it is necessary to use a heat resistant container such as a jar fermenter for the heat treatment. The heating time is appropriately determined depending on the pH, the treatment temperature and the desired viscosity characteristic, but is usually 10 to 120 minutes. In addition, a polysaccharide having a predetermined viscosity can be obtained by sampling the viscosity during the measurement and measuring the viscosity.

In the alkaline heat treatment of the polysaccharide according to the present invention, the aqueous solution concentration of the polysaccharide is 0.01 to 3% by weight,
The concentration is preferably 0.1% by weight to 1% by weight. If the concentration exceeds 3% by weight, the viscosity of the aqueous solution may be high and the handleability may deteriorate. Further, if the concentration is 0.01% by weight or less, the concentration is low and the production efficiency is low, which is not preferable.

If the polysaccharide has been subjected to an alkali heat treatment, subsequently the tip speed of the stirring blade according to the present invention is 2.
A method of cooling while maintaining the rotation speed of 0 m / s or less, or cooling without applying shearing force by stirring is applied. ) Specifically, a method of externally cooling the outside of the jar fermenter container with a refrigerant such as water without stirring the inside of the jar fermenter container, or stirring the refrigerant while passing the refrigerant through a cooling coil in the jar fermenter container. Examples thereof include a method of stirring and cooling the blade at a tip speed of 2.0 m / s or less, and a method of press-fitting into a refrigerant cooled coil (coil for cooling) from a jar fermenter container to cool. For cooling in a large jar fermenter vessel,
Cooling takes time at low tip speed and low stirring speed,
It is not preferable because a polysaccharide having a desired viscosity cannot be obtained. The shape of the stirring blade at the time of stirring and cooling is not particularly limited, and a propeller type, ribbon type, blade type or the like is appropriately selected in consideration of a container such as a jar fermenter. A flexible pipe used for cooling (cooling coil)
The diameter, length, material and shape of the are not particularly limited, and may be appropriately selected in consideration of conditions such as the ultimate cooling temperature, the amount of cooling treatment, and the time. The feature of the present invention is that when cooling the polysaccharide aqueous solution that has been subjected to the alkali heat treatment, there is substantially no shearing force due to stirring or the like, or the shearing force due to stirring or the like is reduced to cool, and preferably ,
This is a method of press-fitting into a flexible pipe (cooling coil) that is not accompanied by shearing force.

The tip speed of the stirring blade during stirring and cooling is 2.0 m.
If it exceeds / s, the increase in viscosity of the polysaccharide obtained by the alkali heat treatment is small, and the change in viscosity with temperature is larger than that of the polysaccharide before the alkali heat treatment, and the change in viscosity with time is also large. It is not preferable. Also,
It is not preferable to continue stirring and cooling more than necessary.

The target cooling temperature is not particularly limited, but it may be appropriately selected usually from 40 ° C. to room temperature.

When the cooling temperature of the aqueous solution of the polysaccharide subjected to the alkali heat treatment reaches 40 ° C. to room temperature, an aqueous solution of an inorganic acid (sulfuric acid, hydrochloric acid) is added to neutralize it, and then about 3 times the amount is added with stirring. By adding (volume) of an organic solvent such as acetone, ethanol, or isopropyl alcohol, the heat-treated polysaccharide is precipitated as an agglomerate, and the target thickener is easily isolated by filtration and drying under reduced pressure. Be done.

The polysaccharide which has been subjected to the alkali heat treatment has a high thickening property in a small amount (by cooling the polysaccharide in a manner not accompanied by strong shearing force), and further, the emulsion stability of the emulsified product and the dispersion of the solid dispersed product. Due to its excellent stability, it has become applicable to a wide range of fields such as cosmetics, pharmaceuticals, foods, paints, inks and agricultural chemicals.

The thickener of the present invention may be used in a mixture with other thickeners as long as the effects of the present invention are not impaired.

Since the polysaccharide used in the present invention is an extremely high molecular weight polymer having a molecular weight of 10 million or more, when it is treated at a high temperature in the form of an aqueous alkaline solution, the molecule becomes elongated and the viscosity increases. However, when the mixture is vigorously stirred and subjected to shearing force during cooling, the extended molecular chain is cut by shearing force, and part of the three-dimensional structure of the molecule is changed, resulting in a decrease in viscosity and a thickening effect. It is thought to disappear.

[0034]

EXAMPLES The present invention will be specifically described below with reference to examples. However, the present invention is not limited to these.

[Culture of Alcaligenes latorus B-16 strain bacterium] Alcaligenes latorus B-16 (FER
MBP2015) strain with meat extract 0.5%, peptone 1
%, Sodium chloride 0.3%, inoculated into 1 L of medium,
After shaking culture for 24 hours, 200 L of a culture medium having the following composition was put into a 300 L jar fermenter using this as a seed, and aerated and stirred at 30 ° C. for 144 hours (aeration amount: 200
L / min, agitation number: 250 rpm) were cultured.

<Composition of culture medium> Glucose [Wako Pure Chemical Industries, Ltd. reagent] 4.00 kg Dipotassium hydrogen phosphate [Wako Pure Chemical Industries, Ltd. reagent] 0.40 kg potassium dihydrogen phosphate [Japanese Kojun Pure Chemicals Co., Ltd. Reagent] 0.20kg Sodium chloride [Wako Pure Chemicals Co., Ltd. Reagent] 0.01kg Magnesium Sulfate [Wako Pure Chemicals Co., Ltd. Reagent] 0.02kg Potassium Nitrate [Wako Pure Chemicals Industry] Co., Ltd. reagent] 0.10 kg yeast extract [manufactured by Oxoid (OXOID)] 0.15 kg The above components are dissolved in ion-exchanged water, and the pH is adjusted to 6.5 using sodium hydroxide and sulfuric acid. To 200
It was liter.

[Preparation of B-16 Polysaccharide] After the completion of the culture, about 3 volumes of isopropyl alcohol were added to the culture, and the mixture was stirred and mixed. The precipitated aggregate was filtered, collected, and dried under reduced pressure. A B-16 polysaccharide was obtained.

[Preparation of Polysaccharide-1] 199.9 kg of water was placed in a 300 liter container, 100 g of the B-16 polysaccharide obtained by the above method was added and dissolved under stirring to prepare a 0.5 wt% aqueous solution. 200 kg was prepared. This was adjusted to pH 13 with a 1 mol / L sodium hydroxide aqueous solution, and then 3
It was placed in a 00 liter jar fermenter and left overnight at room temperature. After standing, pressure heating treatment was carried out at 120 ° C. for 20 minutes, then cooling to 100 ° C. and returning to normal pressure to obtain alkali heat treated B-16 polysaccharide. While cooling the outside of a stainless steel flexible tube (inner diameter 20 mm, length 12.5 m) with cold water at 15 ° C., the alkali-heated B-16 polysaccharide was passed through the pressure system for 30 minutes and cooled to 30 ° C. Then, it was neutralized with 1 mol / L sulfuric acid. After neutralization, about 3 times the volume of the aqueous polysaccharide solution was added to isopropyl alcohol, and the precipitated aggregates were filtered and dried under reduced pressure to obtain polysaccharide-1.

[Preparation of Polysaccharide-2] 99.95 g of water was placed in a 500-liter container, 0.5 g of the B-16 polysaccharide obtained by the above method was added and dissolved under stirring to obtain 0.5 wt. % 100% aqueous solution was prepared. This was adjusted to pH 13 with a 1 mol / L sodium hydroxide aqueous solution, put into a 30 liter jar fermenter, and left overnight at room temperature. I left it. It was heated at 120 ° C. for 20 minutes under pressure, then allowed to stand, cooled to room temperature, and neutralized with 1 mol / L sulfuric acid. After neutralization, about 3 times the volume of the polysaccharide aqueous solution was added to isopropyl alcohol, and the precipitated aggregate was filtered and dried under reduced pressure to obtain alkali-treated polysaccharide-2.

[Preparation of Polysaccharide-3] In a 30 liter container, 19.99 kg of water was added, and 10 g of the B-16 polysaccharide obtained by the above method was added and dissolved under stirring to give 0.5% by weight.
20 kg of aqueous solution was prepared. This was adjusted to pH 13 with a 1 mol / L sodium hydroxide aqueous solution, put into a 30 liter jar fermenter, and left overnight at room temperature. Heat the jar fermenter to 120 ° C for 2
Pressure heating treatment was performed for 0 minutes. Then, after cooling to 100 ° C. and returning to normal pressure, cold water of 15 ° C. was passed through the jacket of the jar fermenter for 6 hours for cooling. Further, it was neutralized with 1 mol / L sulfuric acid, isopropyl alcohol in an amount about 3 times the volume of the polysaccharide aqueous solution was added, and the precipitated aggregate was filtered and dried under reduced pressure to obtain alkali-treated polysaccharide-3. .

[Production of Polysaccharide-4] In the method for producing the alkali-heat-treated Polysaccharide-1, the obtained alkali-heat-treated B-16 polysaccharide was placed in a jacket of a 30-liter jar fermenter containing 15 It is cooled by passing cold water of ℃ at the same time, using a stirring device with a propeller-type stirring blade at the same time, stirring at a tip speed of 1.88 m / S for 2 hours and stirring at 30 °
It cooled below and neutralized with 1 mol / L sulfuric acid. After neutralization, about 3 times the volume of the polysaccharide aqueous solution was added to isopropyl alcohol, and the precipitated aggregates were filtered and dried under reduced pressure to obtain alkali-treated polysaccharide-4. Similarly, under the cooling conditions shown in Table 1, polysaccharide-7 was obtained with a 30 liter jar fermenter.

[Production of Polysaccharide-5] 199.9 kg of water was placed in a 300 liter container, 100 g of the B-16 polysaccharide obtained by the above method was added and dissolved under stirring, and a 0.5 wt% aqueous solution was prepared. 200 kg was prepared. This was adjusted to pH 13 with a 1 mol / L sodium hydroxide aqueous solution, and then 3
It was placed in a 00 liter jar fermenter and left overnight at room temperature. Heat the jar fermenter, 12
Pressure heat treatment at 0 ° C for 20 minutes, then 100
After cooling to ℃ and returning to normal pressure, cold water at 15 ℃ was passed through the jacket of the jar fermenter, and at the same time, using a stirring device with a propeller-type stirring blade, the tip speed was 1.88 m / s.
The mixture was stirred for 2 hours and cooled to below 30 ° C. Further, the mixture was neutralized with 1 mol / L sulfuric acid, isopropyl alcohol in an amount about 3 times the volume of the polysaccharide aqueous solution was added, and the precipitated aggregate was filtered and dried under reduced pressure to obtain polysaccharide-5. Similarly, polysaccharides-8 and 9 were prepared under the cooling conditions shown in Table 1. In addition, polysaccharide-
6 is a B-16 polysaccharide that has not been treated with alkali.

[Manufacture of Polysaccharide-10] A 0.5% by weight aqueous solution of xanthan gum (trademark, manufactured by Sansho Co., Ltd.) was prepared as a thickener-10.

[Production of Polysaccharide-11] Polysaccharide-11 was prepared by substituting xanthan gum (trademark, manufactured by Sansho Co., Ltd.) for B-16 polysaccharide in the method for producing Polysaccharide-1.
Got

[0045]

[Table 1]

[Viscosity Measurement of Thickener Aqueous Solution] To a 200 mL tall beaker, 0.2 g of a thickener and 199.8 g of water were added, and the mixture was stirred and dispersed at 8000 rpm for 10 minutes with a small homomixer to prepare a 0.1 wt% aqueous solution. And The aqueous solution was immersed in each of the constant temperature water layers of 30 ° C., 50 ° C. and 80 ° C., allowed to stand for 24 hours, then brought to 30 ° C., and a B type rotational viscometer (30 rp).
m, and measured 1 minute after the start of rotation). The results are shown in Table 2.

[0047]

[Table 2]

When cooling the B-16 polysaccharide after the alkali heat treatment, a shearing force of 2.0 with or without stirring is applied.
The thickener of the present invention prepared by cooling at 0 m / s or less is cooled under the condition that the tip speed by stirring exceeds 2.00 m / s.
It can be seen that the viscosity is higher than those of the prepared Comparative Examples 7 to 9, the viscosity is maintained even at high temperature, and the change with time due to temperature is small. In particular, the thickener-1 obtained by a cooling method using a flexible tube (cooling coil) without shearing force and the thickener-2 obtained by cooling by standing in a 500 m container are both B
It can be seen that the viscosity of the -16 polysaccharide is higher than that of -16 polysaccharide, and the change with time of the viscosity from a low temperature to a high temperature region is small. Also, with xanthan gum, no thickening effect was observed even by the same alkali heat treatment and cooling method without stirring.

[Measurement of emulsification / dispersion stability] 200 mL
Thor beaker 96mg thickener, water 159.904g
Was added, and the mixture was stirred and dispersed at 8000 rpm for 20 minutes with a small homomixer to obtain a 0.06 wt% aqueous solution. Liquid paraffin (0.8 g) was added thereto, and the mixture was stirred and dispersed at 8000 rpm for 10 minutes with a small homomixer, a part of the liquid was taken, the absorbance at 500 nm was measured, and the measured value was set to A. After measuring the absorbance, about 40 mL each was placed in two glass sample bottles having a diameter of 2.5 mm and left at 30 ° C. and 50 ° C. Then, after 1 week and 2 months, a sample was taken from the bottom of the sample bottle with a Pasteur pipette, and the absorbance at 500 nm was measured and designated as B. The ratio of the absorbance A immediately after the preparation of the sample solution: A and the absorbance B of the sample after standing for a predetermined period was evaluated as the emulsification / dispersion stability rate (%) as in the following formula, and the higher the ratio, the better. The results are shown in Table 3. Emulsification / dispersion stabilization rate (%) = {(B) / (A)} × 10
0

[0050]

[Table 3]

It can be seen that the thickener of the present invention prepared without stirring or with a small shearing force by stirring during cooling after the alkali heat treatment is excellent in emulsion / dispersion stability.

[0052]

INDUSTRIAL APPLICABILITY The thickener of the present invention shows a high viscosity with a small amount, maintains the viscosity even at high temperature and for a long time, and is excellent in emulsion / dispersion stability as compared with the conventional thickeners. . Therefore, it can be suitably used for thickening and emulsifying / dispersing and stabilizing emulsified products such as cosmetics, pharmaceuticals, foods, paints, inks and agricultural chemicals.

   ─────────────────────────────────────────────────── ─── Continued front page    (72) Inventor Tomomi Kuromiya             Mie prefecture Yokkaichi city aka 6-6-9 Hakuto             Yokkaichi Institute Inc. (72) Inventor Keisuke Hisayoshi             81, No. 11772, Nooka, Saiki City, Oita Prefecture (72) Inventor Toyoya Fumiya             65-2 Mejima, Saiki City, Oita Prefecture F term (reference) 4C083 AD211 EE01 EE07 FF01

Claims (5)

[Claims] 1. A polysaccharide substantially containing fucose, glucose, glucuronic acid and rhamnose as constituent monosaccharides,
After heat-treating at 60 to 180 ° C in an alkaline aqueous solution having H of 8 or more, cooling is performed while maintaining the tip speed of the stirring blade at a rotation speed of 2.0 m / s or less, or without applying shearing force by stirring. A thickener containing, as an active ingredient, a polysaccharide obtained by a cooling method. 2. The constituent monosaccharides of the polysaccharide are fucose: glucose: glucuronic acid: rhamnose = 1 to 2: in molar ratio.
The thickener according to claim 1, which is 1 to 4: 1 to 2: 1 to 2. 3. The thickener according to claim 1, wherein the polysaccharide has a structure composed of glucose, glucuronic acid, and rhamnose as a main chain, and further has fucose bonded to a side chain. 4. The polysaccharide is Alkaline Genesulators B.
The thickener according to any one of claims 1 to 3, which is a polysaccharide produced by strain -16. 5. The thickener according to any one of claims 1 to 4, wherein the method of cooling without applying shearing force by stirring is a method of passing the cooled flexible tube under pressure.