JP2003043051A - Unit for biochemical analysis - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a unit for biochemical analysis in which data peculiar to the unit for biochemical analysis such as a microarray, a macroarray or the like can be controlled surely and which can enhance the reliability of a biochemical analysis. SOLUTION: The unit 1 for biochemical analysis is provided with a substrate 2, and a data recording layer 5 comprising a data-rewritable recording medium is formed on the substrate.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a biochemical analysis unit, and more specifically, to reliably manage data unique to each biochemical analysis unit such as a microarray or a macroarray. The present invention relates to a biochemical analysis unit capable of significantly improving the reliability of biochemical analysis.

[0002]

2. Description of the Related Art When a radiation is irradiated, the energy of the radiation is absorbed, stored and recorded, and then excited by using an electromagnetic wave of a specific wavelength range. A photostimulable phosphor having the property of emitting a stimulating amount of light is used as a radiation detection material, and a substance having a radioactive label is administered to an organism, and then the organism or tissue of the organism is treated. A portion of the sample is used as a sample, and this sample is overlapped with a stimulable phosphor sheet provided with a stimulable phosphor layer for a certain period of time to store and record radiation energy in the stimulable phosphor. , Scanning the stimulable phosphor layer with electromagnetic waves to excite the stimulable phosphor and photoelectrically detecting the stimulable light emitted from the stimulable phosphor to generate a digital image signal. Image processing, CRT On a recording material such as a display unit or on the photographic film, the autoradiographic analyzing system is configured to reproduce an image has been known (for example, Kokoku 1-70884 and JP Kokoku 1-70
882, Japanese Patent Publication No. 4-3962, etc.).

An autoradiography analysis system using a stimulable phosphor sheet as a radiation detecting material not only requires a chemical treatment called a developing treatment, unlike the case where a photographic film is used, but also was obtained. By subjecting the digital data to data processing, there is an advantage that the analysis data can be reproduced or a quantitative analysis by a computer can be performed as desired.

On the other hand, there is known a fluorescence analysis system using a fluorescent substance such as a fluorescent dye as a labeling substance instead of the radioactive labeling substance in the autoradiography analysis system. According to this fluorescence analysis system, by detecting the fluorescence emitted from the fluorescent substance, the gene sequence, the expression level of the gene, the metabolism, absorption, and excretion routes of the administered substance in the experimental mouse, the state, the separation of the protein, Identification or evaluation of molecular weight and characteristics can be performed. For example, a solution containing plural kinds of protein molecules to be electrophoresed is electrophoresed on the gel support, and then the gel support is subjected to fluorescence. An image is generated by staining the electrophoresed protein by immersing it in a solution containing a dye, exciting the fluorescent dye with excitation light, and detecting the resulting fluorescence, and then producing an image on the gel support. The position and quantitative distribution of protein molecules can be detected. Alternatively, by Western blotting,
A probe and a protein molecule prepared by transferring at least a part of the electrophoresed protein molecule onto a transfer support such as nitrocellulose and labeling an antibody that specifically reacts with the target protein with a fluorescent dye are prepared. By selectively associating and selectively labeling a protein molecule that binds only to an antibody that specifically reacts,
By exciting the fluorescent dye with the excitation light and detecting the generated fluorescence, an image can be generated and the position and quantitative distribution of the protein molecule on the transfer support can be detected. In addition, after adding a fluorescent dye to a solution containing a plurality of DNA fragments to be electrophoresed, the plurality of DNA fragments are electrophoresed on a gel support, or on a gel support containing a fluorescent dye. , A plurality of DNA fragments are electrophoresed, or a plurality of DNA fragments are electrophoresed on a gel support, and then the gel support is immersed in a solution containing a fluorescent dye. DNA fragments are labeled, a fluorescent dye is excited by excitation light, and the resulting fluorescence is detected to generate an image, and the distribution of DNA on the gel support is detected, or a plurality of DNAs are detected.
The fragments are electrophoresed on a gel support, followed by DNA
Denaturation, and then by Southern blotting, at least a part of the denatured DNA fragment is transferred onto a transfer support such as nitrocellulose, and DNA or RNA complementary to the target DNA is fluorescent dye. A probe DNA or probe RN prepared by hybridizing a probe prepared by labeling with
Only the DNA fragment complementary to A is selectively labeled, the fluorescent dye is excited by the excitation light, and the resulting fluorescence is detected to generate an image, so that the DNA of interest on the transfer support is detected. The distribution can be detected. further,
DN containing a target gene labeled with a labeling substance
A DNA probe complementary to A is prepared, hybridized with the DNA on the transcription support, and the enzyme is allowed to bind to the complementary DNA labeled with a labeling substance, and then contacted with a fluorescent substrate for fluorescence. An image is generated by changing the substrate to a fluorescent substance that emits fluorescence, exciting the generated fluorescent substance with excitation light, and detecting the generated fluorescence,
It is also possible to detect the distribution of the target DNA on the transcription support. This fluorescence analysis system has an advantage that gene sequences and the like can be easily detected without using radioactive substances.

Similarly, a substance derived from a living body such as a protein or a nucleic acid is immobilized on a support and is selectively labeled with a labeling substance which causes chemiluminescence by contacting with a chemiluminescent substrate. A selectively labeled biological substance is brought into contact with a chemiluminescent substrate, and chemiluminescence in the visible light wavelength region generated by the contact between the chemiluminescent substrate and the labeled substance is photoelectrically detected to obtain a digital image signal. Chemiluminescence for generating information, reproducing the chemiluminescence image on a display material such as a CRT or a recording material such as a photographic film by performing image processing, and obtaining information on a substance of biological origin such as gene information. Analysis systems are also known.

Further, in recent years, hormones, tumor markers, enzymes, antibodies, antigens, abzymes, etc. have been found at different positions on the surface of a carrier such as a slide glass plate and a membrane filter.
Other proteins, nucleic acids, cDNA, DNA, RNA
Such as specific binding substances that can specifically bind to a substance of biological origin and whose base sequence, base length, composition, etc. are known, are dropped using a spotter device, and a large number of independent Then, a spot is formed, and then hormones, tumor markers, enzymes, antibodies, antigens, abzymes, other proteins, nucleic acids, cDNAs, DNAs, mRNAs, etc. are collected from the living body by extraction, isolation, etc., or, Substances of biological origin that have been subjected to chemical treatment, chemical modification, etc., labeled with a labeling substance such as a fluorescent substance or a dye, can be specifically bound to a specific binding substance by hybridization. The bound microarray is irradiated with excitation light, and light such as fluorescence emitted from a labeling substance such as a fluorescent substance or dye is photoelectrically detected to obtain a substance derived from a living body. Microarray analysis system that analyzes have been developed. According to this microarray analysis system, a large number of spots of specific binding substances are formed at high density at different positions on the surface of a carrier such as a slide glass plate or a membrane filter, and a substance of biological origin labeled with a labeling substance is used. By hybridizing with, there is an advantage that a substance derived from a living body can be analyzed in a short time.

[0007] Further, hormones, tumor markers, enzymes,
Antibodies, antigens, abzymes, other proteins, nucleic acids,
A specific binding substance, such as cDNA, DNA, or RNA, which can be specifically bound to a substance of biological origin and whose base sequence, base length, composition, etc. is known, is dropped using a spotter device. , Multiple independent spots, then hormones, tumor markers, enzymes, antibodies, antigens, abzymes, other proteins, nucleic acids, cDNA, DN
Substances derived from a living body, such as A and mRNA, which have been collected from the living body by extraction, isolation, etc., or which have been further subjected to chemical treatment, chemical modification, etc., and which have been labeled with radiolabeled substances , By hybridization or the like, to the specific binding substance, the macroarray specifically bound, is brought into close contact with the stimulable phosphor sheet on which the stimulable phosphor layer containing the stimulable phosphor is formed, The photostimulable phosphor layer is exposed to light, and then the photostimulable phosphor layer is irradiated with excitation light, and the photostimulable light emitted from the photostimulable phosphor layer is photoelectrically detected for biochemical analysis. A macroarray analysis system using a radiolabeled substance that generates use data and analyzes a substance derived from a living body has also been developed.

[0008]

DISCLOSURE OF THE INVENTION In a microarray analysis system or a macroarray analysis system, if the type and position of the specific binding substance spotted on the carrier have not been grasped, the carrier will be spotted on the carrier. Even if the substance of biological origin is specifically bound to the dropped specific binding substance by hybridization or the like to generate biochemical analysis data, analysis cannot be performed, and therefore, microarray or Data relating to the types of specific binding substances dropped in spots on a biochemical analysis unit such as a macroarray and the dropping position are associated with each biochemical analysis unit such as a microarray or macroarray and managed. There is a need.

Further, when a biochemical analysis unit such as a microarray or a macroarray is repeatedly used, a part of the specific binding substance is separated from the biochemical analysis unit, and the analysis can be performed accurately. Therefore, the number of times the biochemical analysis unit such as a microarray or a macroarray is used also needs to be managed in association with each biochemical analysis unit such as a microarray or a macroarray.

However, it is extremely troublesome to separately manage the data unique to each biochemical analysis unit such as a microarray or macroarray, while the data unique to each biochemical analysis unit is If not properly managed, there was a problem that the reliability of biochemical analysis was significantly reduced.

Therefore, according to the present invention, the data unique to each biochemical analysis unit such as a microarray or a macroarray can be reliably managed, and the reliability of biochemical analysis can be greatly improved. It is intended to provide a chemical analysis unit.

[0012]

The object of the present invention is to:
A biochemical analysis unit comprising a substrate, and a data recording layer including a rewritable recording medium formed on the substrate.

According to the present invention, since the data recording layer including the rewritable recording medium is formed on the substrate of the biochemical analysis unit, the type of the specific binding substance dropped and the dropping position. Related to each biochemical analysis unit, such as data on the number of times the biochemical analysis unit has been used, and other data required to be managed in association with each biochemical analysis unit, are recorded in the data recording layer. Surely with each of the units for
It becomes possible to associate and manage.

In a preferred embodiment of the present invention, a specific binding substance capable of specifically binding to a substance derived from a living body and having a known base sequence, base length, composition, etc. is dropped onto the substrate. Thus, a plurality of spot-shaped regions are formed.

In a preferred aspect of the present invention, the data recording layer is formed of a magnetic recording medium.

According to a preferred embodiment of the present invention, since the data recording layer is formed of a magnetic recording medium, the biochemical analysis unit, such as hybridization, retains the data even when subjected to a liquid treatment. can do.

In a preferred aspect of the present invention, the data recording layer is formed of an optical recording medium.

According to a preferred embodiment of the present invention, the data recording layer is formed of an optical recording medium,
The data can be retained even when the biochemical analysis unit such as hybridization undergoes treatment with a liquid.

[0019] In a preferred aspect of the present invention, the data recording layer has a first data recording area in which data cannot be rewritten by the user and a second data recording area in which data can be rewritten by the user. Is included.

According to a preferred embodiment of the present invention, the data recording layer has a first data recording area in which the user cannot rewrite data and a second data recording area in which the user can rewrite data. Therefore, it should not be overwritten by the user's free will, such as data on the type of specific binding substance dropped and the dropping position, and data on the number of times the biochemical analysis unit is used. By configuring such that the data essential for management of the data is recorded in the first data recording area of the data recording layer,
The unit for biochemical analysis is guaranteed to be used as desired, and the reliability of the biochemical analysis can be improved, and the user can collect the data personally needed in the data recording layer. Since the data can be written in the second data recording area, the efficiency of biochemical analysis can be significantly improved.

[0021] In a further preferred aspect of the present invention, the first data recording area of the data recording layer comprises:
Data are recorded regarding the type of specific binding substance and the position of the spot-like region containing each specific binding substance.

In a further preferred aspect of the present invention, the first data recording area of the data recording layer comprises:
The number of times the biochemical analysis unit is used can be recorded.

When the biochemical analysis unit is used for a predetermined number of times or more, a part of the dropped specific binding substance is separated from the biochemical analysis unit, and the accuracy of the biochemical analysis is remarkably lowered, resulting in reliability. However, according to a further preferred embodiment of the present invention,
Since the user is configured to record the number of times the biochemical analysis unit has been used in the first data recording area of the data recording layer where data cannot be rewritten, the user may mistake the biochemical analysis unit. Thus, it is possible to effectively prevent the use over a predetermined number of times.

[0024] In a further preferred aspect of the present invention, the date of use of the biochemical analysis unit can be recorded in the first data recording area and / or the second data recording area of the data recording layer. Has been done.

According to a further preferred aspect of the present invention, the date of use of the biochemical analysis unit is recordable in the first data recording area and / or the second data recording area of the data recording layer. Therefore, when a substance derived from a living organism labeled with a radiolabeled substance is hybridized with a specific binding substance dropped on the biochemical analysis unit, the date and time when the biochemical analysis unit can be discarded should be confirmed. It becomes possible to manage it.

In a further preferred aspect of the present invention, in the first data recording area of the data recording layer,
The number of recycling times of the biochemical analysis unit can be recorded.

According to a further preferred aspect of the present invention, since the number of times the biochemical analysis unit is recycled can be recorded in the first data recording area of the data recording layer, the manufacturer is able to record the durability of the substrate. According to the characteristics, it is possible to maximize the use of the substrate and realize resource saving, while maintaining the reliability of the biochemical analysis unit.

[0028] In a preferred aspect of the present invention, the substrate is provided with a plurality of absorptive regions which are two-dimensionally spaced from each other.

According to a preferred embodiment of the present invention, since the plurality of absorptive regions are formed on the substrate in a two-dimensional manner with being separated from each other, the specific binding contained in each absorptive region is formed. It is possible to effectively prevent the substance of biological origin to be specifically bound to the substance from specifically binding to the specifically bound substance contained in the adjacent adsorptive region, and thus It is possible to improve the accuracy of chemical analysis.

[0030] In a further preferred aspect of the present invention, the plurality of holes are two-dimensionally formed in the substrate so as to be spaced apart from each other, and the plurality of absorptive regions are provided in the holes, respectively. The material is filled and formed.

[0031] In a further preferred aspect of the present invention, the plurality of through-holes are two-dimensionally formed in the substrate so as to be spaced apart from each other, and the plurality of absorptive regions include an absorptive material containing an absorptive material. A membrane is press formed into the plurality of through holes of the substrate.

According to a further preferred embodiment of the present invention, a plurality of absorptive regions are formed in the biochemical analysis unit by simply press-fitting the absorptive film containing the absorptive material into the plurality of through holes of the substrate. Therefore, the biochemical analysis unit can be easily manufactured.

In a preferred aspect of the present invention, the substrate is provided with a plurality of recesses which are two-dimensionally spaced apart from each other, and the plurality of absorptive regions are respectively formed on the inner wall surface of the recess. Formed of a layer of adsorbent material that has been deposited.

In a preferred aspect of the present invention, the plurality of absorptive regions are formed on the surface of the substrate.

In a preferred embodiment of the present invention, a plurality of protrusions are formed on the substrate so as to be two-dimensionally spaced apart from each other, and the plurality of absorptive regions are respectively provided at the tip portions of the protrusions. It is formed in the vicinity.

In a preferred embodiment of the present invention, the substrate is formed of an adsorptive material, and a porous plate having a plurality of through holes formed therein is adhered to at least one surface of the substrate to form the porous plate. The plurality of absorptive regions are formed by the absorptive material in the plurality of through holes formed in.

In a further preferred aspect of the present invention, both surfaces of the substrate formed of an adsorptive material are
A perforated plate having a plurality of through holes is closely attached.

[0038] According to a further preferred embodiment of the present invention, a biochemical analysis unit is constructed by closely adhering a porous plate having a plurality of through holes to both surfaces of a substrate made of an adsorptive material. Therefore, the biochemical analysis unit can be handled very easily during the dropping of the specific binding substance, the hybridization, and the exposure operation of the stimulable phosphor sheet.

In a preferred embodiment of the present invention, the substrate has 10 or more absorptive regions formed therein.

In a further preferred aspect of the present invention, the substrate is formed with 50 or more absorptive regions.

In a further preferred aspect of the present invention, the substrate is formed with 100 or more absorptive regions.

In a further preferred aspect of the present invention, the substrate is formed with 500 or more absorptive regions.

In a further preferred aspect of the present invention, the substrate has 1000 or more absorptive regions formed therein.

In a further preferred aspect of the present invention, the substrate has 5000 or more absorptive regions formed therein.

In a further preferred aspect of the present invention, the substrate is formed with 10,000 or more absorptive regions.

In a further preferred aspect of the present invention, the substrate has 50,000 or more absorptive regions formed therein.

In a further preferred aspect of the present invention, the substrate is formed with 100,000 or more absorptive regions.

[0048] In a preferred aspect of the present invention, each of the plurality of absorptive regions has a size of less than 5 mm 2.

[0049] In a further preferred aspect of the present invention, each of the plurality of absorptive regions has a size of less than 1 mm 2.

[0050] In a further preferred aspect of the present invention, each of the plurality of absorptive regions has a size of less than 0.5 mm 2.

In a further preferred aspect of the present invention, each of the plurality of absorptive regions has a size of less than 0.1 mm 2.

In a further preferred aspect of the present invention, each of the plurality of absorptive regions has a size of less than 0.05 mm 2.

[0053] In a further preferred aspect of the present invention, each of the plurality of absorptive regions has a size of less than 0.01 mm 2.

In a preferred aspect of the present invention, the plurality of absorptive regions are formed on the substrate at a density of 10 or more per cm 2.

In a further preferred aspect of the present invention, the plurality of absorptive regions are formed on the substrate at a density of 50 or more per cm 2.

In a further preferred aspect of the present invention, the substrate has a plurality of the absorptive regions of 100 /
It is formed with a density of not less than square centimeters.

In a further preferred aspect of the present invention, the substrate has the plurality of absorptive regions of 500 /
It is formed with a density of not less than square centimeters.

In a further preferred aspect of the present invention, the plurality of absorptive regions are formed on the substrate at a density of 1,000 or more per cm 2.

In a further preferred aspect of the present invention, the plurality of absorptive regions are formed on the substrate at a density of 5000 pieces / square centimeter or more.

In a further preferred aspect of the present invention, the plurality of absorptive regions are formed on the substrate at 10,000.
It is formed with a density of at least one piece / square centimeter.

In a preferred embodiment of the present invention, the absorptive regions are regularly formed.

In a preferred embodiment of the present invention, the substrate has a property of attenuating radiation.

According to a preferred embodiment of the present invention, since the substrate of the biochemical analysis unit has a property of attenuating radiation, specific binding to a plurality of absorptive regions of the biochemical analysis unit is achieved. The substance is dropped, and the specific binding substance contained in the multiple absorptive regions is selectively and specifically bound to the substance of biological origin that has been labeled with a radioactive labeling substance. When the stimulable fluorescent layer of the stimulable phosphor sheet is exposed by the selectively contained radioactive labeling substance, the electron beam (β-ray) emitted from the radioactive labeling substance contained in each adsorptive region is Effectively prevent scattering into the substrate of the biochemical analysis unit and incident on the region of the stimulable phosphor layer to be exposed by the electron beam (β-ray) emitted from the adjacent adsorptive region. Can therefore be electronic It is possible to effectively prevent the noise caused by the scattering of the X-ray (β-ray) from being generated in the biochemical analysis data, so that it is possible to generate the biochemical analysis data with excellent quantitativeness. It will be possible.

In a preferred embodiment of the present invention, when the radiation is transmitted through the substrate by a distance equal to the distance between the adsorbing regions adjacent to each other, the energy of the radiation is reduced to 1/5. It has the following property of damping.

[0065] In a further preferred aspect of the present invention, when the radiation is transmitted through the substrate by a distance equal to the distance between the adsorbing regions adjacent to each other, the energy of the radiation is reduced to 1 / It has the property of being attenuated to 10 or less.

[0066] In a further preferred aspect of the present invention, when the radiation is transmitted through the substrate by a distance equal to the distance between the adsorbing regions adjacent to each other, the energy of the radiation is reduced to 1 / It has the property of being attenuated to 50 or less.

[0067] In a further preferred aspect of the present invention, when the radiation is transmitted through the substrate by a distance equal to the distance between the adsorbing regions adjacent to each other, the energy of the radiation is reduced to 1 / It has the property of being attenuated to 100 or less.

In a further preferred aspect of the present invention, when the radiation is transmitted through the substrate by a distance equal to the distance between the adsorbing regions adjacent to each other, the energy of the radiation is reduced to 1 / It has the property of being attenuated to 500 or less.

[0069] In a further preferred aspect of the present invention, when the radiation is transmitted through the substrate by a distance equal to the distance between the adsorbing regions adjacent to each other, the energy of the radiation is reduced to 1 / It has the property of being attenuated to 1000 or less.

[0070] In a preferred aspect of the present invention, the substrate has a property of attenuating light.

According to a preferred embodiment of the present invention, since the substrate of the biochemical analysis unit has a property of attenuating light, specific binding to a plurality of absorptive regions of the biochemical analysis unit is achieved. Select a substance of biological origin labeled with a labeling substance that causes chemiluminescence by dropping a substance and contacting a specific binding substance contained in a plurality of adsorptive regions with a fluorescent substance or a chemiluminescent substrate. Specifically, when a plurality of adsorptive regions are specifically bound to each other and excitation light is irradiated to photoelectrically detect fluorescence emitted from the fluorescent substance to generate biochemical analysis data, The chemiluminescence emitted from the active region photoelectrically,
When generating data for biochemical analysis, the fluorescence or chemiluminescence emitted from each adsorptive region should be scattered within the substrate and mixed with the fluorescence or chemiluminescence emitted from the adjacent adsorptive regions. It is possible to effectively prevent, and therefore, it is possible to effectively prevent the noise caused by the scattering of fluorescence or chemiluminescence from being generated in the data for biochemical analysis. It becomes possible to generate data for biochemical analysis.

In a preferred aspect of the present invention, when light is transmitted through the substrate by a distance equal to the distance between the adsorbing regions adjacent to each other, the energy of the light is reduced to 1/5. It has the following property of damping.

In a further preferred aspect of the present invention, when the substrate transmits light through the substrate by a distance equal to the distance between the adsorbing regions adjacent to each other, the energy of light is reduced to 1 / It has the property of being attenuated to 10 or less.

In a further preferred aspect of the present invention, when the substrate transmits light through the substrate by a distance equal to the distance between the adsorbing regions adjacent to each other, the energy of the light is reduced to 1 / It has the property of being attenuated to 50 or less.

[0075] In a further preferred aspect of the present invention, when the substrate transmits light through the substrate by a distance equal to the distance between the adsorbing regions adjacent to each other, the energy of light is reduced to 1 / It has the property of being attenuated to 100 or less.

In a further preferred aspect of the present invention, when the substrate transmits light through the substrate by a distance equal to the distance between the adsorbing regions adjacent to each other, the energy of the light is reduced to 1 / It has the property of being attenuated to 500 or less.

In a further preferred aspect of the present invention, when the substrate transmits light through the substrate by a distance equal to the distance between the adsorbing regions adjacent to each other, the energy of light is reduced to 1 / It has the property of being attenuated to 1000 or less.

In a preferred embodiment of the present invention, the porous plate has a property of attenuating radiation.

According to a preferred embodiment of the present invention, since the perforated plate of the biochemical analysis unit has a property of attenuating radiation, the biochemical analysis unit has a specific absorptive region specific to a plurality of absorptive regions. A binding substance is dropped, and a specific binding substance contained in a plurality of adsorptive regions, a substance of biological origin labeled with a labeling substance that causes chemiluminescence by contacting with a fluorescent substance or a chemiluminescent substrate, Selectively or specifically binding, irradiating excitation light to a plurality of absorptive regions, photoelectrically detecting fluorescence emitted from the fluorescent substance, and generating biochemical analysis data or When the chemiluminescence emitted from the absorptive region is photoelectrically detected and the data for biochemical analysis is generated, the fluorescence or chemiluminescence emitted from each absorptive region is scattered in the perforated plate and is adjacent to each other. It is possible to effectively prevent the fluorescence or chemiluminescence emitted from the adhesive region from being mixed with each other, and therefore, noise caused by the scattering of the fluorescence or chemiluminescence is generated in the data for biochemical analysis. Since it is possible to effectively prevent this, it becomes possible to generate biochemical analysis data with excellent quantitativeness.

[0080] In a preferred aspect of the present invention, when the perforated plate transmits radiation through the perforated plate by a distance equal to a distance between the adsorbing regions adjacent to each other,
It has the property of attenuating the energy of radiation to 1/5 or less.

In a further preferred aspect of the present invention, when the perforated plate penetrates through the perforated plate by a distance equal to the distance between the adsorbing regions adjacent to each other, the energy of the radiation is: It has the property of being attenuated to 1/10 or less.

In a further preferred aspect of the present invention, when the perforated plate transmits radiation through the perforated plate by a distance equal to the distance between the adsorbing regions adjacent to each other, the energy of the radiation is: It has the property of being attenuated to 1/50 or less.

In a further preferred aspect of the present invention, when the perforated plate transmits radiation through the perforated plate by a distance equal to the distance between the adsorbing regions adjacent to each other, the energy of the radiation is: It has the property of being attenuated to 1/100 or less.

In a further preferred aspect of the present invention, when the perforated plate transmits the radiation through the perforated plate by a distance equal to the distance between the adsorbing regions adjacent to each other, the energy of the radiation is It has the property of being attenuated to 1/500 or less.

[0085] In a further preferred aspect of the present invention, when the perforated plate penetrates through the perforated plate by a distance equal to the distance between the adsorbing regions adjacent to each other, the energy of the radiation is: It has the property of being attenuated to 1/1000 or less.

In a preferred embodiment of the present invention, the porous plate has a property of attenuating light.

According to a preferred embodiment of the present invention, since the perforated plate of the biochemical analysis unit has a property of attenuating light, it is specific to a plurality of absorptive regions of the biochemical analysis unit. A binding substance is dropped, and a specific binding substance contained in a plurality of adsorptive regions, a substance of biological origin labeled with a labeling substance that causes chemiluminescence by contacting with a fluorescent substance or a chemiluminescent substrate, Selectively or specifically binding, irradiating excitation light to a plurality of absorptive regions, photoelectrically detecting fluorescence emitted from the fluorescent substance, and generating biochemical analysis data or The chemiluminescence emitted from the adsorptive region is detected photoelectrically,
When generating data for biochemical analysis, the fluorescence or chemiluminescence emitted from each adsorptive area must be scattered within the perforated plate and mixed with the fluorescence or chemiluminescence emitted from the adjacent adsorbent areas. Therefore, it is possible to effectively prevent the noise due to the scattering of fluorescence or chemiluminescence from being generated in the data for biochemical analysis, and therefore, it has excellent quantification. It becomes possible to generate data for biochemical analysis.

In a preferred aspect of the present invention, when the perforated plate transmits light through the perforated plate for a distance equal to the distance between the adsorbing regions adjacent to each other, the light energy is set to 1 It has the property of being attenuated to / 5 or less.

In a further preferred aspect of the present invention, when the perforated plate transmits light through the perforated plate for a distance equal to the distance between the adsorbing regions adjacent to each other, the energy of the light is changed to: It has the property of being attenuated to 1/10 or less.

In a further preferred aspect of the present invention, when the perforated plate transmits light through the perforated plate for a distance equal to the distance between the adsorbing regions adjacent to each other, the energy of the light is changed to: It has the property of being attenuated to 1/50 or less.

In a further preferred aspect of the present invention, when the perforated plate transmits light through the perforated plate for a distance equal to the distance between the adsorbing regions adjacent to each other, the energy of the light is changed to: It has the property of being attenuated to 1/100 or less.

In a further preferred aspect of the present invention, when the perforated plate transmits light through the perforated plate for a distance equal to the distance between the adsorbing regions adjacent to each other, the energy of the light is changed to: It has the property of being attenuated to 1/500 or less.

In a further preferred aspect of the present invention, when the perforated plate transmits light through the perforated plate for a distance equal to the distance between the adsorbing regions adjacent to each other, the energy of the light is: It has the property of being attenuated to 1/1000 or less.

In the present invention, the material for forming the substrate or porous plate of the biochemical analysis unit preferably has a property of attenuating radiation, and has a property of attenuating light. However, it is not particularly limited, and either an inorganic compound material or an organic compound material can be used, and a metal material, a ceramic material or a plastic material is preferably used.

In the present invention, as the inorganic compound material which can be preferably used for forming the substrate or the porous plate of the biochemical analysis unit, for example, gold,
Metals such as silver, copper, zinc, aluminum, titanium, tantalum, chromium, iron, nickel, cobalt, lead, tin and selenium; alloys such as brass, stainless steel and bronze; silicon, amorphous silicon, glass, quartz, silicon carbide, Examples thereof include silicon materials such as silicon nitride; metal oxides such as aluminum oxide, magnesium oxide, and zirconium oxide; inorganic salts such as tungsten carbide, calcium carbonate, calcium sulfate, hydroxyapatite, and gallium arsenide. These may have any structure in a polycrystalline sintered body such as single crystal, amorphous, or ceramic.

In the present invention, a polymer compound is preferably used as the organic compound material that can be used to form the substrate or porous plate of the biochemical analysis unit,
As the polymer compound that can be preferably used,
For example, polyolefins such as polyethylene and polypropylene; acrylic resins such as polymethylmethacrylate and butylacrylate / methylmethacrylate copolymers; polyacrylonitrile; polyvinyl chloride; polyvinylidene chloride; polyvinylidene fluoride; polytetrafluoroethylene; polychlorotrifluoro Ethylene; Polycarbonate; Polyester such as polyethylene naphthalate and polyethylene terephthalate; Nylon such as Nylon 6, Nylon 6,6, Nylon 4, 10; Polyimide; Polysulfone; Polyphenylene sulfide; Silicon resin such as polydiphenylsiloxane; Phenolic resin such as novolac Epoxy resin; polyurethane;
Polystyrene; butadiene-styrene copolymers; polysaccharides such as cellulose, cellulose acetate, nitrocellulose, starch, calcium alginate, hydroxypropylmethyl cellulose; chitin; chitosan; sumac; polyamides such as gelatin, collagen, keratin and the like of these polymer compounds Examples thereof include copolymers.
These may be a composite material, if necessary, can be filled with metal oxide particles or glass fiber,
It is also possible to blend and use an organic compound material.

Generally, the higher the specific gravity is, the higher the radiation attenuating ability is. Therefore, the substrate or the porous plate of the biochemical analysis unit should be formed of a compound material or a composite material having a specific gravity of 1.0 g / cm ³ or more. Is preferable, and it is particularly preferable that the specific gravity is 1.5 g / cm ³ or more and 23 g / cm ³ or less.

In general, the larger the light scattering and / or absorption, the higher the light attenuating ability. Therefore, the substrate or porous plate of the biochemical analysis unit has an absorbance of 0.3 or more per cm of thickness. Preferably, the thickness is 1
More preferably, the absorbance per cm is 1 or more. Here, the absorbance is measured immediately after the plate-shaped body having a thickness of Tcm,
It is determined by placing an integrating sphere, measuring the transmitted light amount A at the wavelength of probe light or emission light used for measurement with a spectrophotometer, and calculating A / T. In order to improve the light attenuating ability, a light scatterer or a light absorber may be contained in the substrate or the porous plate of the biochemical analysis unit. Fine particles of a material different from the material forming the substrate or porous plate of the biochemical analysis unit are used as the light scatterer, and a pigment or dye is used as the light absorber.

In a preferred aspect of the present invention, data relating to the plurality of absorptive areas is recordable in the first data recording area of the data recording layer.

When a plurality of absorptive regions are formed on a substrate so as to be separated from each other, it is extremely difficult to form all absorptive regions in the same size. However, although noise is generated due to the inability to form a plurality of absorptive regions uniformly, according to a preferred embodiment of the present invention, the first portion of the data recording layer is formed.
Since the data recording area is configured to be able to record data relating to a plurality of absorptive areas,
It is possible to remove noise by correcting the biochemical analysis data based on the data relating to the multiple absorptive areas recorded in the data recording area of, and therefore, the biochemical analysis data with excellent quantitativeness. Can be generated.

In a preferred embodiment of the present invention, the substrate is an absorptive substrate formed of an absorptive material.

In the present invention, a porous material or a fibrous material is preferably used as the absorptive material forming the absorptive region or the absorptive substrate of the biochemical analysis unit. The porous material and the fiber material may be used together to form the adsorptive region.

In the present invention, the porous material used to form the adsorptive region of the biochemical analysis unit or the adsorptive substrate may be either an organic material or an inorganic material, or an organic / inorganic composite. .

In the present invention, the organic porous material used for forming the absorptive region or the absorptive substrate of the biochemical analysis unit is not particularly limited, but it is a carbon material such as activated carbon or a membrane. A material capable of forming a filter is preferably used. Specifically, nylons such as nylon 6, nylon 6,6, nylon 4,10; nitrocellulose, cellulose acetate,
Cellulose derivatives such as cellulose acetate butyrate; collagen; alginic acid, calcium alginate, alginic acid /
Alginic acids such as polylysine polyion complex; polyolefins such as polyethylene and polypropylene; polyvinyl chloride; polyvinylidene chloride; polyfluoride such as polyvinylidene fluoride and polytetrafluoride; and copolymers or composites thereof. .

In the present invention, the inorganic porous material used for forming the adsorptive region or the adsorptive substrate of the biochemical analysis unit is not particularly limited, but preferably, for example, platinum, Examples thereof include metals such as gold, iron, silver, nickel and aluminum; metal oxides such as alumina, silica, titania and zeolite; metal salts such as hydroxyapatite and calcium sulfate, and complexes thereof.

In the present invention, the fibrous material used for forming the adsorptive region or the adsorptive substrate of the biochemical analysis unit is not particularly limited, but preferably, for example, nylon 6 or nylon. Nylons such as 6,6 and nylon 4,10, nitrocellulose,
Examples thereof include cellulose derivatives such as cellulose acetate and cellulose butyrate.

In a preferred embodiment of the present invention, the substrate is a slide glass plate.

[0108]

Preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

FIG. 1 is a schematic perspective view of a biochemical analysis unit according to a preferred embodiment of the present invention.

As shown in FIG. 1, the biochemical analysis unit 1 according to this embodiment includes a substrate 2 formed of aluminum and having a large number of substantially circular through holes 3 formed therein at a high density. Nylon 6 is filled inside the large number of through holes 3 to form a large number of dot-shaped absorptive regions 4
Are formed.

Although not shown exactly in FIG. 1, in the present embodiment, approximately 500 through-holes 3 having a substantially circular shape having a size of about 0.01 square millimeter of about 10,000 are provided.
Substrate 2 regularly with a density of 0 pieces / cm 2.
Is formed in. The absorptive region 4 has a large number of through holes 3 so that its surface is located at the same height as the surface of the substrate 2.
Nylon 6 is filled inside and formed.

As shown in FIG. 1, on the substrate 2 of the biochemical analysis unit 1, a magnetic recording layer 5 is formed by a magnetic recording medium, and two circular positioning through holes 6a,
6b is formed.

The magnetic recording layer 5 is divided into a first data recording area 5a in which data cannot be written by the user and a second data recording area 5b in which data can be written by the user.

In this embodiment, the absorptive region 4 is formed by filling the large number of through holes 3 formed in the substrate 2 of the biochemical analysis unit 1 with the absorptive material. It is difficult to form the through-holes 3 in a uniform size, and it is also difficult to uniformly fill the through-holes 3 with the adsorptive material. Therefore, as described below, the radiation data or fluorescence data is recorded in a large number of absorptive regions 4, and the biochemical analysis data is obtained by reading the radiation data or fluorescence data. Noise may be generated due to the non-uniform amount of the adsorptive material contained in the created adsorptive region 4.

Therefore, in the present embodiment, the size variation of a large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1 is measured using an electron microscope or the like, and a laser displacement meter is used. The position of the surface of the absorptive material filled in each through hole 3 is detected by using the measured amount of the absorptive material filled in each through hole 3, and a plurality of absorptive regions 4 are formed. As data regarding the amount of the adsorptive material contained, a magnetic recording head (not shown) is used.
It is configured to record in the first data recording area 5a of the magnetic recording layer 5 formed in the biochemical analysis unit 1.

First data recording area 5a of magnetic recording layer 5
Further, the type of specific binding substance dropped by the spotting device, the position of the absorptive region 4 on which each is dropped, the number of times the biochemical analysis unit 1 is used, etc. are recorded. There is.

FIG. 2 is a schematic front view of the spotting device.

In the biochemical analysis, as shown in FIG. 2, in a large number of absorptive regions 4 regularly formed in the biochemical analysis unit 1, for example, as a specific binding substance, the base sequence is Multiple known different cDNAs
Are dropped using a spotting device.

As shown in FIG. 2, the spotting device comprises an injector 7 and a CCD camera 8 for injecting a solution of a specific binding substance toward the biochemical analysis unit 1.
It has a spotting head 9 equipped with.

FIG. 3 is a schematic plan view of the spotting device.

As shown in FIG. 3, the spotting device is provided with a driving mechanism, and the driving mechanism of the spotting device is, for example, the biochemical analysis unit 1 on which a specific binding substance such as cDNA is to be dropped. Substrate 1 to be placed
It is attached to a frame 11 fixed to 0.

As shown in FIG. 3, on the frame 11, the sub-scanning pulse motor 12 and the pair of rails 13, 13 are provided.
3 are fixed, and a substrate 14 that is movable on the frame 11 along the pair of rails 13 and 13 in the sub-scanning direction indicated by the arrow Y in FIG. 3 is further provided.

A threaded hole (not shown) is formed in the movable substrate 14, and a threaded rod 15 rotated by the sub-scanning pulse motor 12 is formed in this hole. Engaged.

A main scanning pulse motor 16 is provided on the movable substrate 14, and the main scanning pulse motor 16 can drive the endless belt 17 intermittently at a predetermined pitch.

The spotting head 9 of the spotting device is fixed to the endless belt 17, and when the endless belt 17 is driven by the main scanning pulse motor 16, in the main scanning direction indicated by the arrow X in FIG. It is configured to be moved.

In FIG. 3, 18 is a linear encoder for detecting the position of the spotting head 9 in the main scanning direction, and 19 is a slit of the linear encoder 18.

As shown in FIG. 3, on the substrate 10 of the spotting device, two spots are provided at positions corresponding to the two through holes 6a and 6b for positioning formed on the substrate 2 of the biochemical analysis unit 1. Positioning pins 20a, 20b are provided upright, so that the two positioning pins 20a, 20b formed on the substrate 10 of the spotting device are inserted into the corresponding positioning through holes 6a, 6b. The analysis unit 1 is mounted on the substrate 10 of the spotting device.
By placing it on top, it is guaranteed that the biochemical analysis unit 11 is always placed at approximately the same position on the substrate 10 of the spotting device.

FIG. 4 is a block diagram showing the control system, input system, drive system, detection system and recording system of the spotting device.

As shown in FIG. 4, the control system of the spotting device has a control unit 25 for controlling the operation of the entire spotting device, and the input system of the spotting device has a keyboard 26.

The drive system of the spotting device comprises a main scanning pulse motor 16 and a sub-scanning pulse motor 12, and the detection system of the spotting device is a linear encoder 18 for detecting the position of the spotting head 9 in the main scanning direction. A rotary encoder 17 for detecting the rotation amount of the rod 15 and a CCD camera 8 are provided.

As shown in FIG. 4, the recording system of the spotting device has a magnetic recording head 27.

With the spotting device configured as described above, a large number of absorptive regions 4 formed in the biochemical analysis unit 1 according to this embodiment are carried out as follows.
Then, a specific binding substance such as cDNA is dropped.

First, the two positioning pins 20a and 20b formed on the substrate 10 of the spotting device are inserted into the corresponding two through holes 6a and 6b for positioning of the biochemical analysis unit 1, respectively. Then, the biochemical analysis unit 1 is placed on the substrate 10 of the spotting device.

Next, data concerning the type of the specific binding substance to be dropped and the position of the absorptive region 4 to which each should be dropped by the operator, the biochemical analysis unit 1 thereof and the specific stimulable phosphor sheet. When and are provided to the user as one biochemical analysis kit, together with the biochemical analysis unit 1,
Identification data for specifying the stimulable phosphor sheet that constitutes one biochemical analysis kit and is used together is input to the keyboard 26. The input data is input to the control unit 25. The stimulable phosphor sheet will be described later.

In the present embodiment, the data of the type of the specific binding substance to be dropped and the position of the adsorptive region 4 to which each is dropped are patterned and stored in a memory (not shown). The operator specifies the type of the specific binding substance to be dropped and the pattern of the data of the position of the adsorptive region 4 to which each is dropped, and the control unit 25 reads the corresponding pattern from the memory. , The operation of the spotting device is controlled.

On the keyboard 26 by the operator,
The data on the type of the specific binding substance to be dropped and the position of the adsorptive region 4 to be dropped, and the biochemical analysis unit 1 and the specific stimulable phosphor sheet are used for one biochemical analysis. When provided as a kit to the user, it constitutes one biochemical analysis kit together with the biochemical analysis unit 1, and the identification data for identifying the stimulable phosphor sheet to be used together is inputted. The control unit 25 outputs a write signal to the magnetic recording head 27 based on the input signal, and stores and stores data regarding the type of specific binding substance to be dropped and the position of the adsorptive region 4 to be dropped. The first data recording area of the data recording layer 5 in which the user cannot write identification data for identifying the fluorescent phosphor sheet. To write to a.

As described above, in this embodiment, the biochemical analysis unit 1 is the substrate 10 of the spotting device.
Although it is configured to be placed on a substantially constant position above, in the present embodiment, since the size of the absorptive region 4 is about 0.01 mm 2, it is thus placed on the substrate 10. It is not guaranteed that the centers of the many absorptive regions 4 of the biochemical analysis unit 1 thus prepared are accurately aligned in the main scanning direction and the sub scanning direction of the spotting head 9.

Therefore, in the spotting apparatus according to this embodiment, the relative position between the position of the biochemical analysis unit 1 placed on the substrate 10 and the moving position of the spotting head 9 in the main scanning direction and the sub scanning direction is determined. The positional relationship is detected in advance, and the spotting head 9 is moved by the main scanning pulse motor 16 and the sub-scanning pulse motor 12 so that the specific binding substance is accurately ejected to each porous region 4 by the injector 7. Is configured.

Next, when the operator inputs the spotting start signal to the keyboard 26 and inputs the spotting start signal to the control unit 25, the control unit 25 outputs a drive signal to the main scanning pulse motor 16 and outputs the reference signal. The spotting head 9 located at the position is moved in the main scanning direction indicated by an arrow X in FIG. 3, and then a drive signal is output to the sub-scanning pulse motor 12 to cause the spotting head 9 to move.
Is moved in the sub-scanning direction indicated by arrow Y in FIG.

Thus, the spotting head 9 is moved in the main scanning direction indicated by arrow X in FIG.
While moving in the sub-scanning direction indicated by, the control unit 25 monitors the detection signal input from the CCD camera 8, detects the positions of the four corners of the biochemical analysis unit 1, and detects the position of the spotting head 9. Using the reference position as the origin of the coordinate system, the coordinate values of the four corners of the biochemical analysis unit 1 are calculated and stored in a memory (not shown).

When the positions of the four corners of the biochemical analysis unit 1 are detected and the coordinate values are stored in the memory, the control unit 25 causes the coordinate values of the four corners of the biochemical analysis unit 1 to be detected. Based on, the coordinate value of each absorptive region 4 formed in the biochemical analysis unit 1 is calculated with the reference position of the spotting head 9 as the origin of the coordinate system,
Stored in a memory (not shown).

When the coordinate values of the large number of absorptive regions 4 formed in the biochemical analysis unit 1 are calculated and stored in the memory, the control unit 25 causes the main scanning pulse motor 16 and the sub scanning pulse motor 12 to operate. A drive signal is output to and the spotting head 9 is returned to the original reference position.

Injector 7 of spotting head 9
When the specific binding substance released from is accurately dropped at the position facing the tip of the injector 7, the raw position determined with the reference position of the spotting head 9 as the origin of the coordinate system is as described above. Chemical analysis unit 1
The specific binding substance is released from the injector 7 of the spotting head 9 on the basis of the coordinate value of each absorptive region 4 of each of the absorptive regions 4 of FIG. Although the binding substance can be dripped accurately, the specific binding substance released from the injector 7 of the spotting head 9 is displaced from the position facing the tip of the injector 7 in the X direction and / or the Y direction. When the specific binding substance is released from the injector 7 of the spotting head 9 based on the coordinate value of each absorptive region 4 of the biochemical analysis unit 1 as described above, The specific binding substance cannot be accurately dropped onto each of the absorptive regions 4 formed in the biochemical analysis unit 1.

Therefore, in this embodiment, as shown in FIG. 5, the specific binding is further performed from the injector 7 of the spotting head 9 returned to the reference position toward the surface of the biochemical analysis unit 1. Release the substance,
The position where the specific binding substance is dropped is detected by the CCD camera 8, and based on the detection signal of the CCD camera 8, the control unit 25 causes the deviation amount δx in the X direction from the position 0 facing the tip of the injector 7. And Y
The deviation amount δy in the direction is calculated and stored in the memory.

Here, the displacement amount δx in the X direction and the displacement amount δy in the Y direction from the position 0 facing the tip of the injector 7 at the position where the specific binding substance is dropped are the injector 7 of each spotting head 9. Therefore, when the spotting head 9 is located at a position other than the reference position, the dropping position of the specific binding substance released from the injector 7 toward the surface of the biochemical analysis unit 1 is also: From the position 0 facing the tip of the injector 7, the displacement is δx in the X direction and δy in the Y direction.

Then, the control unit 25 thus makes the coordinate values of the four corners of the biochemical analysis unit 1 stored in the memory and the coordinates of the large number of absorptive regions 4 formed in the biochemical analysis unit 1. Based on the value and the deviation amount δx in the X direction and the deviation amount δy in the Y direction of the dropping position of the specific binding substance, the tip end portion of the injector 7 of the spotting head 9 is spotted at a position facing each absorptive region 4. The drive pulse to be applied to the main scanning pulse motor 16 and the sub scanning pulse motor 12 in order to move the head 9 is calculated, and the drive pulse data is stored in the memory.

Here, in the present embodiment, a large number of absorptive regions 4 of the biochemical analysis unit 1 are formed inside the through holes 3 regularly formed in the substrate 2,
In order to move the spotting head 9 to the position where the tip portion of the injector 7 is opposed to the adsorptive region 4 where the specific binding substance is to be dropped after the third, the main scanning pulse motor 16 and the sub scanning pulse motor 12 are provided. The driving pulse to be given to the spotting device should be dropped second from the position where the tip of the injector 7 of the spotting device faces the adsorptive region 4 where the specific binding substance should be dropped first. It is the same as the drive pulse to be applied to the main scanning pulse motor 16 and the sub scanning pulse motor 12 in order to move the spotting head 9 to the position facing the absorptive region 4, and therefore the tip of the injector 7 is In order to move the spotting head 9 to a position facing the adsorptive region 4 where the specific binding substance should be dropped, The drive pulse to be applied to the inspection pulse motor 16 and the sub-scanning pulse motor 12 and the tip of the injector 7 are second and specific from the position facing the adsorptive region 4 where the specific binding substance is to be dropped. At a position facing the adsorptive region 4 on which the binding substance should be dropped,
It is sufficient to calculate the driving pulse to be applied to the main scanning pulse motor 16 and the sub scanning pulse motor 12 in order to move the spotting head 9 and store it in the memory.

Driving pulses to be given to the main scanning pulse motor 16 and the sub-scanning pulse motor 12 in order to move the spotting head 9 to the position where the tip end portion of the injector 7 faces each absorptive region 4 are calculated, When the drive pulse data is stored in the memory, the control unit 25 gives a predetermined drive pulse to the main scanning pulse motor 16 and the sub-scanning pulse motor 12 based on the drive pulse data stored in the memory, and the spotting head 9 Are intermittently moved, and when the tip portion of the injector 6 reaches a position facing each absorptive region 4 formed in the biochemical analysis unit 1, the main scanning pulse motor 16 and the sub scanning pulse motor 12 Output a drive stop signal to stop the spotting head 9 and And outputs an addition signal to Njekuta 7 ejects the specific binding agent.

Injector 7 of spotting head 9
In the case where the spotting head 9 is moved to a position where the tip end of the second facing the adsorptive region 4 where the specific binding substance should be dropped after the second, the spotting head 9 is moved.
Are moved at a constant pitch in the main scanning direction indicated by arrow X and in the sub-scanning direction indicated by arrow Y.

Similarly, the main scanning pulse motor 16 and the sub-scanning pulse motor 12 intermittently move the spotting head 9, and the spotting head 9 is formed in the biochemical analysis unit 1 in accordance with the instruction signal input by the operator. A predetermined specific binding substance is sequentially dropped onto each of the large number of adsorptive regions 4.

In this way, after the specific binding substance is dropped on the large number of absorptive regions 4 of the biochemical analysis unit 1, the biochemical analysis unit 1 is shipped and supplied to the user.

When receiving the supply of the biochemical analysis unit 1 in which the specific binding substance is dripped on a large number of adsorptive regions 4, the user uses a reader (not shown) to detect the biochemical analysis unit 1. The data recorded on the magnetic recording layer 5 is read and dropped on a large number of adsorptive regions 4 of the biochemical analysis unit 1 to confirm the type and position of the specific binding substance adsorbed, and then the label The substance derived from the living body labeled with the substance is selectively hybridized with the specific binding substance dropped on the large number of adsorptive regions 4.

FIG. 5 is a schematic side view of the hybridization device.

As shown in FIG. 5, the hybridization apparatus 30 includes a cartridge loading section 32 for loading the biochemical analysis unit 1 into the cartridge 31, and the biochemical analysis unit 1 in the cartridge loading section 32. A pretreatment solution, a hybridization solution, a probe solution containing a substance derived from a living body labeled with a labeling substance, and a washing solution are stored in a cartridge 31 housed therein.
A cartridge 31 in which a solution injecting section 33 for selectively injecting and a biochemical analysis unit 1 are accommodated and in which a pretreatment liquid, a hybridization solution, a solution obtained by adding a probe solution to the hybridization solution, or a washing solution is injected A probe solution is added to the pretreatment solution and the hybridization solution from the reaction section 34 which shakes and vibrates and the cartridge 31, and the prepared solution or washing solution is extracted and the biochemical analysis unit 1 is taken out. A chemical analysis unit takeout unit 35 is provided.

FIG. 6 is a schematic perspective view of the cartridge 31.

As shown in FIG. 6, the cartridge 31
Includes a casing 31a and a lid 31b. The lid 31b is filled with a pretreatment liquid, a hybridization solution, a probe solution, and a washing solution into the cartridge 31,
A solution injection / withdrawal port 31c that can be withdrawn is formed.

As shown in FIG. 5, the cartridge loading section 32 of the hybridization device 30 has the first endless belt 36a on which the biochemical analysis unit 1 is set and the first endless belt 36a. ,
In FIG. 5, a pair of pulleys 36b and 36c that are selectively rotatable clockwise and counterclockwise, and a first magnetic recording layer 5 of the biochemical analysis unit 1 set on the first endless belt 36a. 1 data recording area 5a
A read head 37 for reading the data recorded in
A magnetic recording head 38 for writing data on the magnetic recording layer 5 of the biochemical analysis unit 1 and a lid 3 of the cartridge 31.
1b is opened and closed to load the biochemical analysis unit 1 in the cartridge 31; a second endless belt 40a that conveys the cartridge 31 loaded with the biochemical analysis unit 1; Endless belt 4
It has a pair of pulleys 40b and 40c around which 0a is wound.

Further, as shown in FIG. 5, the solution injection part 33 of the hybridization device 30 is connected to the second endless belt 40a of the cartridge loading part 32,
Third endless belt 4 for receiving the cartridge 31
1a, a pair of pulleys 41b and 41c around which the third endless belt 41a is wound, and a pretreatment liquid for solution injection /
A pretreatment liquid injection pin 42 for injecting into the cartridge 31 located at the solution injection position via the extraction port 31c,
The hybridization solution injection pin 43 for injecting the hybridization solution into the cartridge 31 located at the solution injection position through the solution injection / extraction port 31c, and the probe solution for the solution injection / extraction port 31c
The probe solution injection pin 44, which is injected into the cartridge 31 located at the solution injection position via the, and the washing solution, and the washing solution are inserted into the cartridge 31 located at the solution injection position through the solution injection / extraction port 31c. A cleaning solution injection pin 45 for injecting into the inside 31 is provided.

Here, the pair of pulleys 41b and 41c are
A motor (not shown) is configured to be selectively rotatable clockwise and counterclockwise in FIG.

Further, as shown in FIG. 5, the pretreatment liquid injection pin 42 and the hybridization solution injection pin 4
3, the probe solution injection pin 44 and the cleaning solution injection pin 45 are fixed to the solution pin head 46, and the solution pin head 46 is moved by a motor (not shown) along a pair of rails (not shown). It is configured to be possible.

As shown in FIG. 5, the reaction part 34 of the hybridization device 30 is the third part of the solution injection part 33.
The endless belt 41a of FIG. 5 receives the cartridge 31 and transfers the cartridge 31 to the third endless belt 41a of the solution injecting section 33 and the fourth endless belt 47a and the fourth endless belt 47a are wound, At clockwise and counterclockwise,
A pair of selectively rotatable pulleys 47b, 47c and a vibration table 48 for vibrating the fourth endless belt 47a are provided.

Further, as shown in FIG. 5, the biochemical analysis unit take-out section 35 of the hybridization device 30 includes the fourth endless belt 47 of the reaction section 34.
The cartridge 31 is received from a and the cartridge 3
The fifth endless belt 49a and the fifth endless belt 49a that deliver 1 to the fourth endless belt 47a of the reaction section 34 are wound, and selectively rotate clockwise and counterclockwise in FIG. From the inside of the cartridge 31 via a pair of rotatable pulleys 49b, 49c, an RI sensor 50 for detecting the concentration of the radioactive labeling substance contained in the cleaning solution in the cartridge 31, and a solution injection / extraction port 31c. A solution extraction pin 51 for extracting a prepared solution or a cleaning solution by adding a probe solution to a pretreatment solution or a hybridization solution;
A biochemical analysis unit take-out mechanism 52 for taking out the biochemical analysis unit 1 from the cartridge 31 by opening the lid 31b of the cartridge 31 is provided.

FIG. 7 shows a hybridization device 30.
2 is a block diagram of a control system, a detection system, a drive system, an input system, and a display system of.

As shown in FIG. 7, the control system of the hybridization device 30 is a control unit 60 for controlling the operation of the entire hybridization device 30.
And the detection system of the hybridization device 30 is
A read head 37 for reading data recorded on the magnetic recording layer 5 of the biochemical analysis unit 1, and a cartridge 3.
The RI sensor 50 for detecting the amount of the radiolabeled substance contained in the cleaning solution in 1 is provided.

As shown in FIG. 7, the drive system of the hybridization device 30 includes a pair of pulleys 36b and 36b.
c and the first motor 61 that drives the first endless belt 36a, and the pair of pulleys 40b and 40c.
To rotate the second endless belt 40a, a second motor 62 for driving the second endless belt 40a, a pair of pulleys 41b, 41c for rotating the third endless belt 41a, and a pair of A fifth motor 64 that rotates the pulleys 46b and 46c to drive the fourth endless belt 46a and a fifth motor 64 that rotates the pair of pulleys 48b and 48c to drive the fifth endless belt 48a.
Motor 65, the vibration table motor 66 for driving the vibration table 47, the pretreatment liquid injection pin 42, the hybridization solution injection pin 43, the probe solution injection pin 44, and the cleaning solution pin 45 selectively in the cartridge 31. The injection pin motor 67 for moving the solution pin head 46 along a pair of rails (not shown) so as to face the solution injection / extraction port 31c and the RI sensor 50 are arranged in the cartridge 31 located at the solution extraction position.
The RI sensor motor 68 for moving between the detection position inside the cartridge 31 and the retracted position retracted from the cartridge 31, the solution extraction pin 51, the solution suction position inside the cartridge 31 located at the solution extraction position, and the inside of the cartridge 31. A pretreatment liquid is supplied from a pretreatment liquid tank (not shown) that stores the pretreatment liquid to the pretreatment liquid injection pin motor 69 that moves the pretreatment liquid to a retreat position retracted from the pretreatment liquid. A liquid pump 70, a hybridization solution tank (not shown) containing a hybridization solution, and a hybridization solution pump 71 for supplying the hybridization solution to the hybridization solution injection pin 43, and a living body labeled with a labeling substance. Probe solution containing a probe solution containing a substance of origin From the top (not shown) to the probe solution injection pin 44, the probe solution pump 72 that supplies the probe solution, and the wash solution tank (not shown) that stores the wash solution from the wash solution pin 45 to the wash solution pin 45. A cleaning solution pump 73 for supplying the cleaning solution and a solution extracting pin 51 are used to remove the prepared hybridization solution or cleaning solution from the cartridge 31 by adding the probe solution to the pretreatment solution or the hybridization solution. A pump 74, a valve (not shown) for connecting a pretreatment liquid recovery tank (not shown) for collecting the pretreatment liquid to the solution extraction pin 51, a probe solution was added to the hybridization solution, and the solution was prepared. Hybridization solution collection tank (not shown) for collecting the solution and the solution extraction pin 5 A valve opening / closing mechanism for selectively opening / closing a valve (not shown) for communicating with the cleaning solution recovery tank (not shown) for collecting the cleaning solution and a valve (not shown) for communicating the solution extracting pin 51 with each other. 75, a loading mechanism 39 for opening and closing the lid 31b of the cartridge 31 to load the biochemical analysis unit 1 into the cartridge 31, and a lid 31b of the cartridge 31.
To open the biochemical analysis unit 1 and the cartridge 3
Biochemical analysis unit take-out mechanism 52 taken out from No. 1
A magnetic recording head 38 for writing data is provided on the magnetic recording layer 5 of the biochemical analysis unit 1.

As shown in FIG. 7, the input system of the hybridization device 30 is equipped with a keyboard 80, and the display system of the hybridization device 30 is equipped with a display panel 81.

[0167] The hybridization apparatus 30 configured as described above uses the specific binding substance adsorbed on the large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1 as follows. Then, a substance derived from a living body labeled with a labeling substance is selectively hybridized.

First, a hybridization solution is prepared and accommodated in a hybridization solution tank (not shown), and a cleaning solution is prepared and accommodated in a cleaning solution (not shown).

On the other hand, a probe solution containing a substance of biological origin labeled with a labeling substance is prepared and housed in a probe solution chip (not shown).

In the case of selectively labeling a specific binding substance such as cDNA with a radiolabeling substance, a probe solution containing a substance of biological origin which is a probe labeled with the radiolabeling substance is prepared, and a probe solution chip is prepared. Housed inside.

On the other hand, by using a labeling substance that causes chemiluminescence by contacting with a chemiluminescent substrate, the cDNA is
In the case of selectively labeling a specific binding substance such as, a probe solution containing a substance derived from a living organism, which is a probe labeled with a labeling substance that causes chemiluminescence by contacting with a chemiluminescent substrate, is prepared, It is housed in the probe solution chip.

Furthermore, in the case of selectively labeling a specific binding substance such as cDNA with a fluorescent substance such as a fluorescent dye, a probe containing a substance derived from a living body which is a probe labeled with a fluorescent substance such as a fluorescent dye. A solution is prepared and housed in the probe solution chip.

[0173] A substance derived from a living body labeled with a radioactive labeling substance, a substance derived from a living body labeled with a labeling substance that causes chemiluminescence by contact with a chemiluminescent substrate, and a living body labeled with a fluorescent substance such as a fluorescent dye Of the substances derived from the living body, a probe solution containing two or more living body-derived substances can be prepared and housed in the probe solution chip. In the present embodiment, the substance derived from the living body labeled with the radioactive labeling substance A probe solution containing a substance of biological origin labeled with a fluorescent substance is prepared and housed in the probe solution chip.

For hybridization, c
The biochemical analysis unit 1 in which a specific binding substance such as DNA is adsorbed on a large number of absorptive regions 4 is set by the user on the first endless belt 36a of the cartridge loading section 32, and is then placed on the keyboard 80. , Start signal is input. At the same time, the biologically-derived substance labeled with the radiolabeled substance is used for the biochemical analysis unit 1.
When hybridizing to the specific binding substance contained in the large number of adsorptive regions 4 of the above, the RI labeling signal is input to the keyboard 80 by the user.

The start signal and the RI indicator signal input to the keyboard 80 are output to the control unit 60.

Upon receiving the start signal, the control unit 60 outputs a drive signal to the first motor 61,
By rotating the pair of pulleys 36b and 36c, the first endless belt 36 is driven clockwise in FIG.

When the magnetic recording layer 5 of the biochemical analysis unit 1 set on the first endless belt 36a reaches the position facing the read head 37, the control unit 60 drives the first motor 61. A stop signal is output to stop the first endless belt 36, and the read head 37 prevents the user from writing data to the first data recording area 5 of the magnetic recording layer 5.
The data recorded in a is read.

In the present embodiment, in the first data recording area 5a of the magnetic recording layer 5, data relating to the amount of the absorptive material contained in each absorptive area 4, the type of the specific binding substance and the respective The data on the position of the absorptive region 4 on which is dropped, the data on the number of times the biochemical analysis unit 1 is used, and the biochemical analysis unit 1 and the specific stimulable phosphor sheet are combined into one biochemistry. When provided to the user as an analysis kit, the biochemical analysis unit 1 and the biochemical analysis unit 1 constitute one biochemical analysis kit, and the identification data for identifying the accumulative phosphor sheet used together is recorded. ing.

The data read by the read head 37 is output to the control unit 60, and based on the data input from the read head 37, the biochemical analysis unit 1 has already used the data N times. If it is determined that the
The reverse rotation signal is output to the motor 61 of the pulleys 36b, 36
5 is rotated counterclockwise in FIG. 5, the biochemical analysis unit 1 is sent back to the user, and a message indicating that the biochemical analysis unit 1 should be replaced is displayed on the display panel 81.

This is because when the biochemical analysis unit 1 is used for a predetermined number of times N or more, the specific binding substance adsorbed in the absorptive region 4 is peeled off, the analysis accuracy is remarkably lowered, and the reliability is improved. This is because it is not possible to obtain accurate analysis results. N is set to 2, for example.

The biochemical analysis unit 1 sent back to the user is collected by the manufacturer and provided for recycling.

On the other hand, when it is determined that the biochemical analysis unit 1 has been used less than N times based on the data input from the reading head 37, the control unit 60 further determines A drive signal is output to the motor 61 of the above to move the biochemical analysis unit 1 to a position where the magnetic recording layer 5 faces the magnetic recording head 38.

When the biochemical analysis unit 1 is moved to a position where the magnetic recording layer 5 faces the magnetic recording head 38,
The drive stop signal from the control unit 60 is the first
Output to the motor 61.

Next, the control unit 60 outputs a write signal to the magnetic recording head 38, and the biochemical analysis unit recorded in the first data recording area 5a of the magnetic recording layer 5 where the user cannot write data. The number of times of use of 1 is increased only once, and the date and time at which hybridization is performed is written in the first data recording area 5a of the magnetic recording layer 5 based on the built-in clock, and the RI signal is added together with the start signal. If a signal was being input, indicate that the radiolabeled substance was used,
Writing is performed in the first data recording area 5a of the magnetic recording layer 5.

Upon hybridization, data such as data for identifying a person who has collected a substance derived from a living body to be selectively hybridized with the specific binding substance adsorbed in the absorptive region 4 of the biochemical analysis unit 1 is used. When the data personally required by the user in managing the chemical analysis unit 1 is input to the keyboard 60 by the user, the control unit 60
A write signal is output to the magnetic recording head 38 to write the data in the user-writable second data recording area of the magnetic recording layer 5.

When the writing of data to the magnetic recording layer 5 is completed, the control unit 60 causes the first motor 6
1, the drive signal is output again, the pulleys 36b and 36c are rotated, and the biochemical analysis unit 1 is conveyed to the loading mechanism 39.

In the loading mechanism 39, the cartridge 31
However, the lid 31b is held open, and the biochemical analysis unit 1 is fed into the cartridge 31 by the first endless belt 36.

When the biochemical analysis unit 1 is fed into the cartridge 31, the control unit 60
Outputs a drive stop signal to the first motor 61,
While stopping the driving of the endless belt 36 of
A loading signal is output to the loading mechanism 39, and the cartridge 31
The lid 31b is closed.

Then, the control unit 60 outputs a drive signal to the second motor 62, and in FIG.
Rotate the pulleys 40b and 40c clockwise to
Drive the endless belt 40a of the
5, a drive signal is output to the motor 63, and the pulleys 41b and 41c are rotated clockwise in FIG. 5 to drive the third endless belt 41a.

As a result, the cartridge 31 accommodating the biochemical analysis unit 1 is transferred from the second endless belt 40a of the cartridge loading section 32 to the third endless belt 41a of the solution injection section 33.

When the cartridge 31 is delivered to the third endless belt 41a of the solution injecting section 33, the control unit 60 outputs a drive stop signal to the second motor 62 to cause the second endless belt 40a to move. When the drive is stopped and the cartridge 31 is moved to the solution injection position by the third endless belt 41a, the control unit 60 outputs a drive stop signal to the third motor 63 to stop the cartridge 31.

Then, the control unit 60 outputs a drive signal to the injection pin motor 67 so that the pretreatment liquid injection pin 42 may inject the solution of the cartridge 31 along the solution pin head 46 and a pair of rails (not shown). -Move until the position facing the extraction port 31c is reached.

The pretreatment liquid injection pin 42 is attached to the cartridge 3
When the control unit 60 is moved to a position facing the solution injection / withdrawal port 31c of No. 1, the control unit 60 outputs a drive signal to the pretreatment liquid pump 70, and the pretreatment liquid tank (not shown) outputs the pretreatment liquid. The pretreatment liquid is supplied to the cartridge 31 through the injection pin 42 and the solution injection / extraction port 31c.
Let it inject.

When a predetermined time has passed, the control unit 60 outputs a drive stop signal to the pretreatment liquid pump 70 to stop the injection of the pretreatment liquid into the cartridge 31 and drive the third motor 65. A signal is output to drive the third endless belt 41a.

At the same time, the control unit 60 outputs a drive signal to the fourth motor 64, and in FIG.
Rotate the pulleys 46b and 46c clockwise to move the fourth
The endless belt 46a is driven.

As a result, the cartridge 31 accommodating the biochemical analysis unit 1 is transferred from the third endless belt 41a of the solution injection section 33 to the fourth endless belt 46a of the reaction section 34.

When the cartridge 31 is transferred to the fourth endless belt 46a of the reaction section 34, the control unit 60 outputs a drive stop signal to the third motor 63 to drive the third endless belt 41a. And the cartridge 31 is moved to the approximate center of the reaction section 34 by the fourth endless belt 46a,
The control unit 60 outputs a drive stop signal to the fourth motor 64 to stop the cartridge 31.

Next, the control unit 60 outputs a drive signal to the vibration table motor 66 to vibrate the vibration table 48.

As a result, vibration is applied to the cartridge 31, and all the absorptive regions 4 of the biochemical analysis unit 1 housed in the cartridge 31 are moistened by the pretreatment liquid.

When a predetermined time has passed, the control unit 60 outputs a drive stop signal to the vibration table motor 66 to stop the vibration of the vibration table 48, and the fourth
5, a drive signal is output to the motor 64, and the pulleys 46b and 46c are rotated clockwise in FIG. 5 to drive the fourth endless belt 46a, and a drive signal is output to the fifth motor 65. In FIG. 5, the pulleys 48b and 48c are rotated clockwise to drive the fifth endless belt 48a.

As a result, the cartridge 31 has the reaction part 3
4 from the fourth endless belt 46a to the fifth endless belt 48 of the biochemical analysis unit takeout unit 35.
handed over to a.

When the cartridge 31 is transferred to the fifth endless belt 48a of the biochemical analysis unit take-out section 35, the control unit 60 outputs a drive stop signal to the fourth motor 64, and the fourth motor 64 is driven. When the driving of the endless belt 46a is stopped and the cartridge 31 is moved to the solution extracting position by the fifth endless belt 48a, the control unit 60 causes the fifth unit to move to the fifth position.
The drive stop signal is output to the motor 65 to stop the drive of the fifth endless belt 48a.

Next, the control unit 60 outputs a drive signal to the valve opening / closing mechanism 75 to connect the pretreatment liquid recovery tank (not shown) for recovering the pretreatment liquid with the solution extracting pin 51 (see the figure). (Not shown) is opened, a drive signal is output to the solution extraction pin motor 69, the solution extraction pin 51 is moved to the solution suction position in the cartridge 31, and a drive signal is output to the solution extraction pump 74 to drive the cartridge. The pretreatment liquid in 31 is sucked.

In this way, when the pretreatment liquid in the cartridge 31 is sucked by the solution extraction pump 74 and collected in the pretreatment liquid recovery tank, the control unit 6
0 outputs a reverse drive signal to the fifth motor 65, and
In the above, by rotating the pulleys 48b and 48c counterclockwise to drive the fifth endless belt 48a, a reverse drive signal is output to the fourth motor 64,
In FIG. 5, the pulleys 46b and 46c are rotated counterclockwise.
Is rotated to drive the fourth endless belt 46a.

As a result, the cartridge 31 is changed from the fifth endless belt 48a of the biochemical analysis unit take-out section 35 to the fourth endless belt 46 of the reaction section 34.
handed over to a.

When the cartridge 31 is transferred to the fourth endless belt 46a of the reaction section 34, the control unit 60 outputs a drive stop signal to the fifth motor 65 to drive the fifth endless belt 48a. And the reverse drive signal is output to the third motor 63 to rotate the pulley 41b, counterclockwise in FIG.
41c is rotated to drive the third endless belt 41a.

As a result, the cartridge 31 has the reaction part 3
4 from the fourth endless belt 46a to the solution injection part 3
3 is delivered to the third endless belt 41a.

When the cartridge 31 is delivered to the third endless belt 41a of the solution injecting section 33, the control unit 60 outputs a drive stop signal to the fourth motor 64 to cause the fourth endless belt 46a to move. When the drive is stopped and the cartridge 31 is moved to the solution injection position by the third endless belt 41a, the control unit 60 outputs a drive stop signal to the third motor 63 and the third endless belt 41a. Stop driving.

Next, the control unit 60 outputs a drive signal to the injection pin motor 67, and the hybridization solution injection pin 43 is moved to the cartridge 31 along the solution pin head 46 and a pair of rails (not shown).
The solution is moved until it reaches a position facing the solution injection / extraction port 31c.

When the hybridization solution injection pin 43 is moved to the position facing the solution injection / extraction port 31c of the cartridge 31, the control unit 60 causes the hybridization solution pump 71 to move.
A driving signal is output to the cartridge 31 from the hybridization solution tank (not shown) through the hybridization solution injection pin 43 and the solution injection / extraction port 31c.
Let it inject.

After a predetermined time has passed, the control unit 60 turns on the hybridization solution pump 71.
A drive stop signal is output to stop the injection of the hybridization solution, and a drive signal is output to the third motor 63 to rotate the pulleys 41b and 41c clockwise in FIG. The endless belt 41a is driven.

At the same time, the control unit 60 outputs a drive signal to the fourth motor 64, and in FIG.
Rotate the pulleys 46b and 46c clockwise to move the fourth
The endless belt 46a is driven.

As a result, the cartridge 31 accommodating the biochemical analysis unit 1 is transferred from the third endless belt 41a of the solution injection section 33 to the fourth endless belt 46a of the reaction section 34.

When the cartridge 31 is delivered to the fourth endless belt 46a of the reaction section 34, the control unit 60 outputs a drive stop signal to the third motor 63 to drive the third endless belt 41a. And the cartridge 31 is moved to the approximate center of the reaction section 34 by the fourth endless belt 46a,
The control unit 60 outputs a drive stop signal to the fourth motor 64 to stop the cartridge 31.

Next, the control unit 60 outputs a drive signal to the vibration table motor 66 to vibrate the vibration table 48.

As a result, the cartridge 31 was vibrated, and the hybridization solution was brought into uniform contact with all the absorptive regions 4 of the biochemical analysis unit 1 accommodated in the cartridge 31, and pre-hybridization was performed. To be done.

When a predetermined time has passed, the control unit 60 outputs a drive stop signal to the vibration table motor 66 to stop the vibration of the vibration table 48, and the fourth
The reverse drive signal is output to the motor 64 of FIG.
Rotate the pulleys 46b and 46c counterclockwise,
While driving the fourth endless belt 46a,
A reverse drive signal is output to the third motor 63, and in FIG. 5, the pulleys 41b and 41c are rotated counterclockwise to drive the third endless belt 41a.

As a result, the cartridge 31 has the reaction part 3
4 from the fourth endless belt 46a to the solution injection part 3
3 is delivered to the third endless belt 41a.

When the cartridge 31 is delivered to the third endless belt 41a of the solution injecting section 33, the control unit 60 outputs a drive stop signal to the fourth motor 64 to cause the fourth endless belt 46a to move. When the drive is stopped and the cartridge 31 is moved to the solution injection position by the third endless belt 41a, the control unit 60 outputs a drive stop signal to the third motor 63 and the third endless belt 41a. Stop driving.

Then, the control unit 60 outputs a drive signal to the injection pin motor 67, and the probe solution injection pin 44 causes the solution injection head 44 and the pair of rails (not shown) to inject the solution of the cartridge 31. It is moved until it reaches a position facing the extraction port 31c.

Thus, when the probe solution injection pin 44 is moved to the position facing the solution injection / extraction port 31c of the cartridge 31, the control unit 60
A drive signal is output to the probe solution pump 72, and the probe solution is injected into the cartridge 31 from the probe solution chip (not shown) via the probe solution injection pin 44 and the solution injection / extraction port 31c.

As a result, the probe solution containing the substance of biological origin labeled with the labeling substance is added to the hybridization solution housed in the cartridge 31.

When a predetermined time has passed, the control unit 60 outputs a drive stop signal to the probe solution pump 72 to stop the injection of the probe solution and outputs a drive signal to the third motor 63, In FIG. 5, the pulleys 41b and 41c are rotated clockwise to drive the third endless belt 41a.

At the same time, the control unit 60 outputs a drive signal to the fourth motor 64, and in FIG.
Rotate the pulleys 46b and 46c clockwise to move the fourth
The endless belt 46a is driven.

As a result, the cartridge 31 accommodating the biochemical analysis unit 1 is transferred from the third endless belt 41a of the solution injection section 33 to the fourth endless belt 46a of the reaction section 34.

When the cartridge 31 is transferred to the fourth endless belt 46a of the reaction section 34, the control unit 60 outputs a drive stop signal to the third motor 63 to drive the third endless belt 41a. And the cartridge 31 is moved to the approximate center of the reaction section 34 by the fourth endless belt 46a,
The control unit 60 outputs a drive stop signal to the fourth motor 64 to stop the cartridge 31.

Then, the control unit 60 outputs a drive signal to the vibration table motor 66 to vibrate the vibration table 48.

As a result, the cartridge 31 was vibrated and the hybridization solution was brought into uniform contact with all the absorptive regions 4 of the biochemical analysis unit 1 housed in the cartridge 31 and labeled with the radioactive labeling substance. The substance derived from the living body, which is labeled with the substance derived from the living body and the fluorescent substance contained in the hybridization solution and contained in the hybridization solution, is added to the specific binding substance adsorbed on the large number of adsorbing regions 4. Selectively, the specific binding substance that has been hybridized and adsorbed to the large number of adsorptive regions 4 is selectively labeled with a radioactive labeling substance and a fluorescent substance.

When the predetermined time has passed, the control unit 60 outputs a drive stop signal to the vibration table motor 66 to stop the vibration of the vibration table 48, and the fourth
5, a drive signal is output to the motor 64, and the pulleys 46b and 46c are rotated clockwise in FIG. 5 to drive the fourth endless belt 46a, and a drive signal is output to the fifth motor 65. In FIG. 5, the pulleys 48b and 48c are rotated clockwise to drive the fifth endless belt 48a.

As a result, the cartridge 31 has the reaction part 3
4 from the fourth endless belt 46a to the fifth endless belt 48 of the biochemical analysis unit takeout unit 35.
handed over to a.

When the cartridge 31 is delivered to the fifth endless belt 48a of the biochemical analysis unit take-out section 35, the control unit 60 outputs a drive stop signal to the fourth motor 64, and the fourth motor 64 is driven. When the driving of the endless belt 46a is stopped and the cartridge 31 is moved to the solution extracting position by the fifth endless belt 48a, the control unit 60 causes the fifth unit to move to the fifth position.
The drive stop signal is output to the motor 65 to stop the drive of the fifth endless belt 48a.

Next, the control unit 60 outputs a drive signal to the valve opening / closing mechanism 75, the probe solution is added to the hybridization solution, and the hybridization solution recovery tank and the solution extraction pin 51 for recovering the prepared solution. A valve (not shown) communicating with and is opened, a drive signal is output to the solution withdrawal pin motor 69, the solution withdrawal pin 51 is moved to the solution suction position in the cartridge 31, and the solution withdrawal pump 74 is driven. A signal is output, the probe solution is added to the hybridization solution in the cartridge 31, and the prepared solution is sucked.

When the probe solution is added to the hybridization solution in the cartridge 31 and the prepared solution is sucked by the solution withdrawing pump 74 and collected in the hybridization solution collecting tank, the control unit 60 Fifth motor 65
5 to rotate the pulleys 48b and 48c counterclockwise to drive the fifth endless belt 48a and output a reverse drive signal to the fourth motor 64 in FIG. Then, in FIG. 5, the pulleys 46b and 46c are rotated counterclockwise to drive the fourth endless belt 46a.

As a result, the cartridge 31 is changed from the fifth endless belt 48a of the biochemical analysis unit take-out section 35 to the fourth endless belt 46 of the reaction section 34.
handed over to a.

When the cartridge 31 is transferred to the fourth endless belt 46a of the reaction section 34, the control unit 60 outputs a drive stop signal to the fifth motor 65 to drive the fifth endless belt 48a. And the reverse drive signal is output to the third motor 63 to rotate the pulley 41b, counterclockwise in FIG.
41c is rotated to drive the third endless belt 41a.

As a result, the cartridge 31 has the reaction part 3
4 from the fourth endless belt 46a to the solution injection part 3
3 is delivered to the third endless belt 41a.

When the cartridge 31 is delivered to the third endless belt 41a of the solution injecting section 33, the control unit 60 outputs a drive stop signal to the fourth motor 64 to cause the fourth endless belt 46a to move. When the drive is stopped and the cartridge 31 is moved to the solution injection position by the third endless belt 41a, the control unit 60 outputs a drive stop signal to the third motor 63 and the third endless belt 41a. Stop driving.

Next, the control unit 60 outputs a drive signal to the injection pin motor 67, and the cleaning solution injection pin 45 causes the solution injection / injection of the cartridge 31 along the solution pin head 46 and a pair of rails (not shown). It is moved until it reaches a position facing the extraction port 31c.

When the cleaning solution injection pin 45 is moved to the position facing the solution injection / withdrawal port 31c of the cartridge 31 in this way, the control unit 60 outputs a drive signal to the cleaning solution pump 73 and the cleaning solution tank. The cleaning solution is injected into the cartridge 31 from the cleaning solution injection pin 45 (not shown) and the solution injection / extraction port 31c.

When a predetermined time has passed, the control unit 60 outputs a drive stop signal to the cleaning solution pump 73 to stop the injection of the cleaning solution and a drive signal to the third motor 63, In FIG. 5, the pulleys 41b and 41c are rotated clockwise to drive the third endless belt 41a.

At the same time, the control unit 60 outputs a drive signal to the fourth motor 64, and in FIG.
Rotate the pulleys 46b and 46c clockwise to move the fourth
The endless belt 46a is driven.

As a result, the cartridge 31 accommodating the biochemical analysis unit 1 is transferred from the third endless belt 41a of the solution injection section 33 to the fourth endless belt 46a of the reaction section 34.

When the cartridge 31 is transferred to the fourth endless belt 46a of the reaction section 34, the control unit 60 outputs a drive stop signal to the third motor 63 to drive the third endless belt 41a. And the cartridge 31 is moved to the approximate center of the reaction section 34 by the fourth endless belt 46a,
The control unit 60 outputs a drive stop signal to the fourth motor 64 to stop the cartridge 31.

Next, the control unit 60 outputs a drive signal to the vibration table motor 66 to vibrate the vibration table 48.

As a result, the cartridge 31 is vibrated, and the cleaning solution is uniformly contacted with all the absorptive regions 4 of the biochemical analysis unit 1 accommodated in the cartridge 31, and the absorptive region 4 is washed. To be done.

When a predetermined time has elapsed, the control unit 60 outputs a drive stop signal to the vibration table motor 66 to stop the vibration of the vibration table 48, and the fourth
5, a drive signal is output to the motor 64, and the pulleys 46b and 46c are rotated clockwise in FIG. 5 to drive the fourth endless belt 46a, and a drive signal is output to the fifth motor 65. In FIG. 5, the pulleys 48b and 48c are rotated clockwise to drive the fifth endless belt 48a.

As a result, the cartridge 31 has the reaction part 3
4 from the fourth endless belt 46a to the fifth endless belt 48 of the biochemical analysis unit takeout unit 35.
handed over to a.

When the cartridge 31 is delivered to the fifth endless belt 48a of the biochemical analysis unit take-out portion 35, the control unit 60 outputs a drive stop signal to the fourth motor 64, and the fourth motor 64 is driven. When the driving of the endless belt 46a is stopped and the cartridge 31 is moved to the solution extracting position by the fifth endless belt 48a, the control unit 60 causes the fifth unit to move to the fifth position.
The drive stop signal is output to the motor 65 to stop the drive of the fifth endless belt 48a.

Then, the control unit 60 turns the R
A cleaning solution recovery tank that outputs a drive signal to the I sensor motor 68 to move the RI sensor 50 to a detection position in the cartridge 31 and outputs a drive signal to the valve opening / closing mechanism 75 to recover the cleaning solution. Open the valve (not shown) that communicates with the solution extraction pin 51,
A drive signal is output to the solution extraction pin motor 69 to move the solution extraction pin 51 to the solution suction position in the cartridge 31.

Thus, the RI sensor 50 detects the concentration of the radioactive labeling substance in the cleaning solution contained in the cartridge 31, and the detection signal is output to the control unit 60.

Further, the control unit 60 outputs a drive signal to the solution extracting pump 74 to suck the cleaning solution in the cartridge 31 and recover the cleaning solution in the cleaning solution recovery tank (not shown).

When a predetermined time has passed, the control unit 60 outputs a drive stop signal to the solution withdrawing pump 74 to stop suction of the cleaning solution in the cartridge 31, and outputs a drive signal to the solution withdrawing pin motor 69. The solution extracting pin 51 is retracted to the retracted position retracted from the cartridge 31, and a drive signal is output to the RI sensor motor 68 to retract the RI sensor 50 to the retracted position retracted from the cartridge 31.

On the other hand, the control unit 60 uses the RI
Based on the detection signal input from the sensor 50, the concentration of the radioactive labeling substance in the cleaning solution is compared with the reference concentration of the radioactive labeling substance stored in the memory (not shown), and the radioactive labeling substance in the cleaning solution is compared. If the concentration exceeds the standard concentration of the radiolabeled substance, it is recognized that the adsorptive region 4 is not sufficiently washed and it is necessary to inject the washing solution into the cartridge 31 to continue the washing operation. In the control unit 60, the cleaning solution in the cartridge 31 is
At the time when it is sucked by the solution withdrawing pump 74 and collected in the cleaning solution recovery tank, a reverse drive signal is output to the fifth motor 65 to rotate the pulleys 48b and 48c counterclockwise in FIG. , The fifth endless belt 48a is driven, and a reverse drive signal is output to the fourth motor 64 to rotate the pulleys 46b and 46c counterclockwise in FIG. 5 to rotate the fourth endless belt 46a. Drive.

As a result, the cartridge 31 is changed from the fifth endless belt 48a of the biochemical analysis unit take-out section 35 to the fourth endless belt 46 of the reaction section 34.
handed over to a.

When the cartridge 31 is transferred to the fourth endless belt 46a of the reaction section 34, the control unit 60 outputs a drive stop signal to the fifth motor 65 to drive the fifth endless belt 48a. And outputs a reverse drive signal to the third motor 63 to rotate the pulley 41b, counterclockwise in FIG.
41c is rotated to drive the third endless belt 41a.

As a result, the cartridge 31 has the reaction part 3
4 from the fourth endless belt 46a to the solution injection part 3
3 is delivered to the third endless belt 41a.

When the cartridge 31 is delivered to the third endless belt 41a of the solution injecting section 33, the control unit 60 outputs a drive stop signal to the fourth motor 64, and the control unit 60 outputs the fourth endless belt 46a. When the drive is stopped and the cartridge 31 is moved to the solution injection position by the third endless belt 41a, the control unit 60 outputs a drive stop signal to the third motor 63 and the third endless belt 41a. Stop driving.

When the cartridge 31 is returned to the solution injection position in this way, the control unit 60 outputs a drive signal to the cleaning solution pump 73 again, and the cleaning solution injection pin is supplied from the cleaning solution tank (not shown). 45 and the cleaning solution via the solution injection / extraction port 31c,
It is injected into the cartridge 31.

When a predetermined time has passed, the control unit 60 outputs a drive stop signal to the cleaning solution pump 73 to stop the injection of the cleaning solution and also outputs a drive signal to the third motor 63. In FIG. 5, the pulleys 41b and 41c are rotated clockwise to drive the third endless belt 41a.

At the same time, the control unit 60 outputs a drive signal to the fourth motor 64, and in FIG.
Rotate the pulleys 46b and 46c clockwise to move the fourth
The endless belt 46a is driven.

As a result, the cartridge 31 accommodating the biochemical analysis unit 1 is transferred from the third endless belt 41a of the solution injection section 33 to the fourth endless belt 46a of the reaction section 34.

When the cartridge 31 is delivered to the fourth endless belt 46a of the reaction section 34, the control unit 60 outputs a drive stop signal to the third motor 63 to drive the third endless belt 41a. And the cartridge 31 is moved to the approximate center of the reaction section 34 by the fourth endless belt 46a,
The control unit 60 outputs a drive stop signal to the fourth motor 64 to stop the cartridge 31.

Next, the control unit 60 outputs a drive signal to the vibration table motor 66 to vibrate the vibration table 48.

As a result, the cartridge 31 is vibrated, and the cleaning solution is uniformly contacted with all the absorptive regions 4 of the biochemical analysis unit 1 housed in the cartridge 31, and the absorptive region 4 is washed. To be done.

When the predetermined time has passed, the control unit 60 outputs a drive stop signal to the vibration table motor 66 to stop the vibration of the vibration table 48, and the fourth
5, a drive signal is output to the motor 64, and the pulleys 46b and 46c are rotated clockwise in FIG. 5 to drive the fourth endless belt 46a, and a drive signal is output to the fifth motor 65. In FIG. 5, the pulleys 48b and 48c are rotated clockwise to drive the fifth endless belt 48a.

As a result, the cartridge 31 has the reaction part 3
4 from the fourth endless belt 46a to the fifth endless belt 48 of the biochemical analysis unit takeout unit 35.
handed over to a.

When the cartridge 31 is delivered to the fifth endless belt 48a of the biochemical analysis unit take-out section 35, the control unit 60 outputs a drive stop signal to the fourth motor 64, and the fourth motor 64 is driven. When the driving of the endless belt 46a is stopped and the cartridge 31 is moved to the solution extracting position by the fifth endless belt 48a, the control unit 60 causes the fifth unit to move to the fifth position.
The drive stop signal is output to the motor 65 to stop the drive of the fifth endless belt 48a.

Next, the control unit 60 turns the R
A cleaning solution recovery tank that outputs a drive signal to the I sensor motor 68 to move the RI sensor 50 to a detection position in the cartridge 31 and outputs a drive signal to the valve opening / closing mechanism 75 to recover the cleaning solution. Open the valve (not shown) that communicates with the solution extraction pin 51,
A drive signal is output to the solution extraction pin motor 69 to move the solution extraction pin 51 to the solution suction position in the cartridge 31.

In this way, the concentration of the radioactive labeling substance in the cleaning solution contained in the cartridge 31 is detected by the RI sensor 50, and the detection signal is output to the control unit 60.

Further, the control unit 60 outputs a drive signal to the solution extracting pump 74 to suck the cleaning solution in the cartridge 31 and recover the cleaning solution in the cleaning solution recovery tank (not shown).

When a predetermined time has elapsed, the control unit 60 outputs a drive stop signal to the solution withdrawing pump 74 to stop suction of the cleaning solution in the cartridge 31 and outputs a drive signal to the solution withdrawing pin motor 69. The solution extracting pin 51 is retracted to the retracted position retracted from the cartridge 31, and a drive signal is output to the RI sensor motor 68 to retract the RI sensor 50 to the retracted position retracted from the cartridge 31.

On the other hand, the control unit 60 uses the RI
Based on the detection signal input from the sensor 50, the concentration of the radioactive labeling substance in the cleaning solution is compared with the reference concentration of the radioactive labeling substance stored in the memory (not shown), and the radioactive labeling substance in the cleaning solution is compared. If the concentration exceeds the standard concentration of the radiolabeled substance, it is recognized that the cleaning of the adsorptive region 4 is still insufficient and it is necessary to further inject the cleaning solution into the cartridge 31 to continue the cleaning operation. Therefore, the control unit 60 outputs a reverse drive signal to the fifth motor 65 at the time when the cleaning solution in the cartridge 31 is sucked by the solution withdrawing pump 74 and collected in the cleaning solution recovery tank. 5, the pulleys 48b and 48c are rotated counterclockwise to move the fifth
Drive the endless belt 48a of the
The reverse drive signal is output to the motor 64 of FIG.
Rotate the pulleys 46b and 46c counterclockwise,
The fourth endless belt 46a is driven.

As a result, the cartridge 31 is changed from the fifth endless belt 48a of the biochemical analysis unit take-out section 35 to the fourth endless belt 46 of the reaction section 34.
handed over to a.

When the cartridge 31 is transferred to the fourth endless belt 46a of the reaction section 34, the control unit 60 outputs a drive stop signal to the fifth motor 65 to drive the fifth endless belt 48a. And the reverse drive signal is output to the third motor 63 to rotate the pulley 41b, counterclockwise in FIG.
41c is rotated to drive the third endless belt 41a.

As a result, the cartridge 31 has the reaction part 3
4 from the fourth endless belt 46a to the solution injection part 3
3 is delivered to the third endless belt 41a.

When the cartridge 31 is delivered to the third endless belt 41a of the solution injecting section 33, the control unit 60 outputs a drive stop signal to the fourth motor 64, and the fourth endless belt 46a. When the drive is stopped and the cartridge 31 is moved to the solution injection position by the third endless belt 41a, the control unit 60 outputs a drive stop signal to the third motor 63 and the third endless belt 41a. Stop driving.

In this way, when the cartridge 31 is returned to the solution injection position, the control unit 60 outputs the drive signal to the cleaning solution pump 73 again, and the cleaning solution injection pin is supplied from the cleaning solution tank (not shown). 45 and the cleaning solution via the solution injection / extraction port 31c,
It is injected into the cartridge 31.

When a predetermined time has passed, the control unit 60 outputs a drive stop signal to the cleaning solution pump 73 to stop the injection of the cleaning solution and also outputs a drive signal to the third motor 63. In FIG. 5, the pulleys 41b and 41c are rotated clockwise to drive the third endless belt 41a.

At the same time, the control unit 60 outputs a drive signal to the fourth motor 64, and in FIG.
Rotate the pulleys 46b and 46c clockwise to move the fourth
The endless belt 46a is driven.

As a result, the cartridge 31 accommodating the biochemical analysis unit 1 is transferred from the third endless belt 41a of the solution injection section 33 to the fourth endless belt 46a of the reaction section 34.

When the cartridge 31 is delivered to the fourth endless belt 46a of the reaction section 34, the control unit 60 outputs a drive stop signal to the third motor 63 to drive the third endless belt 41a. And the cartridge 31 is moved to the approximate center of the reaction section 34 by the fourth endless belt 46a,
The control unit 60 outputs a drive stop signal to the fourth motor 64 to stop the cartridge 31.

Next, the control unit 60 outputs a drive signal to the vibration table motor 66 to vibrate the vibration table 48.

As a result, the cartridge 31 is vibrated, and the cleaning solution is uniformly contacted with all the absorptive regions 4 of the biochemical analysis unit 1 housed in the cartridge 31, and the absorptive region 4 is washed. To be done.

When the predetermined time has elapsed, the control unit 60 outputs a drive stop signal to the vibration table motor 66 to stop the vibration of the vibration table 48, and the fourth
5, a drive signal is output to the motor 64, and the pulleys 46b and 46c are rotated clockwise in FIG. 5 to drive the fourth endless belt 46a, and a drive signal is output to the fifth motor 65. In FIG. 5, the pulleys 48b and 48c are rotated clockwise to drive the fifth endless belt 48a.

As a result, the cartridge 31 has the reaction part 3
4 from the fourth endless belt 46a to the fifth endless belt 48 of the biochemical analysis unit takeout unit 35.
handed over to a.

When the cartridge 31 is transferred to the fifth endless belt 48a of the biochemical analysis unit take-out section 35, the control unit 60 outputs a drive stop signal to the fourth motor 64, and the fourth motor 64 is driven. When the driving of the endless belt 46a is stopped and the cartridge 31 is moved to the solution extracting position by the fifth endless belt 48a, the control unit 60 causes the fifth unit to move to the fifth position.
The drive stop signal is output to the motor 65 to stop the drive of the fifth endless belt 48a.

Next, the control unit 60 turns the R
A cleaning solution recovery tank that outputs a drive signal to the I sensor motor 68 to move the RI sensor 50 to a detection position in the cartridge 31 and outputs a drive signal to the valve opening / closing mechanism 75 to recover the cleaning solution. Open the valve (not shown) that communicates with the solution extraction pin 51,
A drive signal is output to the solution extraction pin motor 69 to move the solution extraction pin 51 to the solution suction position in the cartridge 31.

In this way, the concentration of the radiolabeled substance in the cleaning solution contained in the cartridge 31 is detected by the RI sensor 50, and the detection signal is output to the control unit 60.

Further, the control unit 60 outputs a drive signal to the solution extracting pump 74 to suck the cleaning solution in the cartridge 31 and recover the cleaning solution in the cleaning solution recovery tank (not shown).

When a predetermined time has passed, the control unit 60 outputs a drive stop signal to the solution withdrawing pump 74 to stop suction of the cleaning solution in the cartridge 31, and outputs a drive signal to the solution withdrawing pin motor 69. The solution extracting pin 51 is retracted to the retracted position retracted from the cartridge 31, and a drive signal is output to the RI sensor motor 68 to retract the RI sensor 50 to the retracted position retracted from the cartridge 31.

On the other hand, based on the detection signal input from the RI sensor 50 by the control unit 60, the concentration of the radiolabeled substance in the washing solution and the radiolabeled substance reference stored in the memory (not shown). The concentration is compared.

Thus, until the concentration of the radiolabeled substance in the washing solution falls below the radiolabeled substance standard concentration,
When the washing with the washing solution is repeated and the concentration of the radiolabeling substance in the washing solution drops below the radiolabeling substance reference concentration, the control unit 60 determines that the washing is completed and drives the fifth motor 65. A signal is output to rotate the pulleys 48b and 48c clockwise in FIG. 5 to drive the fifth endless belt 48a.

As a result, the fifth endless belt 48a
Thus, the cartridge 31 is sent to the biochemical analysis unit removal mechanism 52.

When the cartridge 31 is sent to the biochemical analysis unit removal mechanism 52, the control unit 60 outputs a drive stop signal to the fifth motor 65 to stop the drive of the fifth endless belt 48a. ,
A drive signal is output to the biochemical analysis unit extraction mechanism 52.

Biochemical analysis unit removal mechanism 52
When the drive signal is received from the control unit 60, opens the lid 31b of the cartridge 31 and takes out the biochemical analysis unit 1 housed in the cartridge 31.

In this way, the radiation data of the radioactive labeling substance as the labeling substance and the fluorescence data of the fluorescent substance such as the fluorescent dye are recorded in the large number of absorptive regions 4 of the biochemical analysis unit 1. The fluorescence data recorded in the absorptive region 4 is read by a scanner described later, and biochemical analysis data is generated.

On the other hand, the radiation data of the radiolabeled substance is
The radiation data transferred to the stimulable phosphor sheet and transferred to the stimulable phosphor sheet is read by a scanner described later to generate biochemical analysis data.

FIG. 8 is a schematic perspective view of the stimulable phosphor sheet.

As shown in FIG. 8, the stimulable phosphor sheet 90 according to the present embodiment has a large number of substantially circular through holes 9.
3 has a nickel-made support 91 formed regularly, and a stimulable phosphor is embedded in a large number of through holes 93 formed in the support 91 to provide a large number of stimulable phosphor layers. The area 92 is formed in a dot shape.

A large number of through holes 93 are formed in the support 91 in the same pattern as the large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1, and each stimulable phosphor layer region. 92 is formed to have the same size as the absorptive region 4 formed on the substrate 2 of the biochemical analysis unit 1.

Therefore, although not shown exactly in FIG. 8, there is about 5 of the substantially circular stimulable phosphor layer region 92 having a size of about 0.01 square millimeter of about 10,000.
The support 91 of the stimulable phosphor sheet 90 has a density of 000 pieces / square centimeter and the same regular pattern as the large number of absorptive regions 4 formed on the substrate 2 of the biochemical analysis unit 1, It is formed in a dot shape.

As shown in FIG. 8, the support 91 includes:
Two positioning through holes 96a and 96b are formed at positions corresponding to the two positioning through holes 6a and 6b formed in the substrate 2 of the biochemical analysis unit 1.

In the present embodiment, the support 91
Surface and dot-shaped stimulable phosphor layer region 9
The support 91 is placed so that the surface of
The stimulable phosphor is embedded in the through-hole 93 formed in the above to form the stimulable phosphor sheet 90, and the support 91 is formed on the substrate 2 of the biochemical analysis unit 1 on the surface thereof. The magnetic recording layer 9 is formed at a position corresponding to the formed magnetic recording layer 5.
4 are formed.

[0304] When the stimulable phosphor sheet 90 and the specific biochemical analysis unit 1 are provided to the user as one biochemical analysis kit, the operator uses the magnetic field of the stimulable phosphor sheet 90. Identification data for identifying the biochemical analysis unit 1 constituting one biochemical analysis kit can be recorded in the recording layer together with the stimulable phosphor sheet.

Here, identification data for identifying the biochemical analysis unit 1 which constitutes one biochemical analysis kit can be recorded on the magnetic recording layer of the stimulable phosphor sheet 90 together with the stimulable phosphor sheet. Consists of a person with abnormal gene expression and is labeled with a radiolabeled substance from the living body selectively to the absorptive region 4 of the biochemical analysis unit 1. The stimulable phosphor layer region 92 of the stimulable phosphor sheet 90 is exposed to light by the radioactive labeling substance which is specifically bound to the adsorbed specific binding substance and is selectively contained in the adsorbing region 4. The obtained biochemical analysis data and a substance derived from a living body, which was collected from a healthy subject and labeled with a radiolabeled substance,
Selectively bound to the specific binding substance adsorbed in the adsorptive region 4 of the biochemical analysis unit 1, and by the radiolabeled substance selectively contained in the adsorptive region 4, the stimulable phosphor When the photostimulable phosphor layer area 92 of the sheet 90 is exposed and the obtained biochemical analysis data is compared to perform an inspection, the same biochemical analysis unit 1 is always used.
And the stimulable phosphor sheet 90 are used to ensure that the inspection is performed and improve the inspection accuracy. Therefore, the magnetic recording layer 94 of the stimulable phosphor sheet 90 is used.
In the case where the identification data for identifying the biochemical analysis unit 1 is recorded in the biochemical analysis unit, the recorded identification data together with the stimulable phosphor sheet 90 constitute one biochemical analysis unit. It has a predetermined correspondence with the identification data recorded on the first magnetic recording layer 5.

In FIG. 9, a large number of photostimulable phosphor layer regions 92 formed on the stimulable phosphor sheet 90 are selectively included in a large number of absorptive regions 4 formed in the biochemical analysis unit 1. It is a schematic perspective view of the exposure apparatus which exposes with the radioactive labeling substance currently provided.

As shown in FIG. 9, the exposure apparatus for the stimulable phosphor sheet 90 according to the present embodiment has the casing 1
00 and a lid member 101, and a substrate 102 on which the biochemical analysis unit 1 and the stimulable phosphor sheet 90 are placed is provided in the casing 100. The casing 100 and the lid member 101 are made of metal having a property of attenuating radiation.

As shown in FIG. 9, the lid member 101 of the exposure apparatus is provided with a data reading / recording unit 103, and the data reading / recording unit 103 includes a reading head (not shown) and magnetic recording. It has a head (not shown).

As shown in FIG. 9, the substrate 1 of the exposure apparatus
02 has two alignment pins 104a and 104b.
Is erected.

FIG. 10 is a schematic partial sectional view showing a mechanism for locking the lid member of the stimulable phosphor sheet exposure apparatus to the casing.

As shown in FIG. 10, the lid member 101
A lid member locking mechanism that locks the above with the casing 100 is provided on both sides of the lid member 101, one of which is shown in FIG. 10.

As shown in FIG. 10, the locking mechanism of the lid member 101 includes a hook member 105 provided inside both side portions of the lid member 101, and the hook member 105 around the shaft 105a in a clockwise direction in FIG. It is provided with a compression spring 106 that biases the casing 100 and an engagement groove 107 provided inside the side plate of the casing 100.

When the lid member 101 is closed, the hook member 105 is biased by the spring force of the compression spring 106 and engages with the engagement groove 107 provided in the casing 100, so that the lid member 101 is closed. It is configured to be locked.

The lid member locking mechanism further includes a lid member 10
1, the hook member 105 is swung counterclockwise in FIG. 10 around the shaft 105a against the urging force of the compression spring 106, so that the hook member 105 and the engaging groove 107 are formed. A solenoid 108 is provided for releasing the engagement with.

FIG. 11 is a block diagram showing a control system, a detection system, a drive system and a display system of the exposure apparatus for the stimulable phosphor sheet.

As shown in FIG. 11, the control system of the exposure apparatus for the stimulable phosphor sheet comprises a control unit 110 for controlling the entire exposure apparatus, and the detection system of the exposure apparatus for the stimulable phosphor sheet is a raw system. Magnetic recording layer 5 of chemical analysis unit 1 and magnetic recording layer 9 of stimulable phosphor sheet 90
4 is provided with a read head 111 of a data reading / recording unit 103 for reading the data recorded in 4, and a read signal of the read head 111 is input to the control unit 110.

As shown in FIG. 11, the drive system of the exposure apparatus for the stimulable phosphor sheet uses the magnetic recording head 112 of the data reading / recording unit 103 for writing data in the magnetic recording layer 94 of the stimulable phosphor sheet 90. And a solenoid 80 for releasing the lid member locking mechanism, and the display system of the exposure apparatus for the stimulable phosphor sheet has a display panel 113 composed of a liquid crystal panel or the like.

Further, the input system of the exposure apparatus for the stimulable phosphor sheet is provided with the lid member opening button 114.

Radioactive label in which the dot-shaped stimulable phosphor layer region 92 formed in the stimulable phosphor sheet 10 is included in the dot-shaped absorptive region 3 formed in the biochemical analysis unit 1. When exposing with a substance, first
The lid member release button 114 is operated.

When the lid member release button 114 is operated,
The lid member opening signal is input to the control unit 110, and when the control unit 110 receives the lid member opening signal, it outputs a drive signal to the solenoid 108.

As a result, the solenoid 108 is driven and the hook member 105 is swung in the clockwise direction in FIG. 10 around the shaft 105a against the biasing force of the compression spring 106 so that the hook member 105 and Engagement groove 107
Is disengaged and the lid member 101 of the exposure apparatus is opened.

When the lid member 101 is opened, the user sets two alignment pins 104a and 104b erected on the substrate 102 of the exposure apparatus on the substrate 2 of the biochemical analysis unit 1 by the user. Through-hole 6 for alignment
The biochemical analysis unit 1 is set on the substrate 102 of the exposure apparatus so as to be inserted into the a and 6b, and the lid member 1
01 is closed.

In the data reading / recording unit 98, the biochemical analysis unit 1 is set on the substrate 102, and the lid member 1
When the lid 01 is closed, the lid member 101 is provided so as to face the magnetic recording layer 5 formed on the substrate 2 of the biochemical analysis unit 1. First, the data reading / recording unit 98 reads the data. Data recorded on the magnetic recording layer 5 formed on the substrate 2 of the biochemical analysis unit 1 is read by the head 111, and a detection signal is output to the control unit 110. When the biochemical analysis unit 1 and the specific stimulable phosphor sheet 90 are provided to the user as one biochemical analysis kit,
Identification data for identifying the stimulable phosphor sheet 90 is recorded on the magnetic recording layer 5 of the biochemical analysis unit 1, and the read head 11 of the data reading / recording unit 98 is recorded.
The identification data read by 1 is output to the control unit 110.

The data input to the control unit 110 is stored in the memory (not shown).

Next, the lid member opening button 114 is operated to open the lid member 101, and the two positioning pins 104a and 104b provided upright on the substrate 102 of the exposure apparatus.
The biochemical analysis unit 1 is set on the substrate 102 so that the biochemical analysis unit 1 is inserted into the two alignment through holes 96a and 96b formed in the support 91 of the stimulable phosphor sheet 90. The stimulable phosphor sheet 90 is set on the surface of the analysis unit 1, and the lid member 101 is closed.

The magnetic recording layer 94 is a stimulable phosphor sheet 9
Since it is formed at a position corresponding to the magnetic recording layer 5 of the biochemical analysis unit 1 on the support 91 of No. 0, the read head 111 of the data reading / recording unit 98 uses the magnetic recording of the stimulable phosphor sheet 90. The data recorded on the layer 94 can be read, and the stimulable phosphor sheet 90 is read by the read head 111 of the data reading / recording unit 98.
The data recorded on the magnetic recording layer 94 of
The detection signal is output to the control unit 110.

Here, the stimulable phosphor layer region 92 of the stimulable phosphor sheet 90 is repeatedly exposed to a radioactive labeling substance, and is excited by excitation light to be contained in the stimulable phosphor layer region 92. If a fluorescent substance is excited and the emitted photostimulant light is detected to generate biochemical analysis data, the accuracy of analysis will be significantly reduced, and reliable analysis results will not be obtained. When the body sheet 90 is set in the exposure device and exposed by the radioactive labeling substance contained in the adsorptive region 4 of the biochemical analysis unit 1, the number of exposures is determined by the magnetic field of the stimulable phosphor sheet 90. The number of exposures recorded on the magnetic recording layer 94 is read by the reading head 111 of the data reading / recording unit 98 and output to the control unit 110. It is.

On the other hand, when the stimulable phosphor sheet 90 and the specific biochemical analysis unit 1 are provided to the user as one biochemical analysis kit, the magnetic recording of the stimulable phosphor sheet 90 is performed. Identification data identifying the biochemical analysis unit 1 is recorded in the layer 94, and the identification data read by the read head 111 of the data reading / recording unit 98 is recorded in the control unit 11.
It is output to 0.

When the identification data of the biochemical analysis unit 1 and the identification data of the stimulable phosphor sheet 90 are input from the reading head 111 of the data reading / recording unit 98, the control unit 110 receives the input identification. Based on the data, when it is determined that the biochemical analysis unit 1 and the stimulable phosphor sheet 90 set in the exposure apparatus are not permitted to be used together,
The drive signal is output to the solenoid 108 and the display signal is output to the display panel 113.

As a result, the solenoid 108 is driven and the hook member 105 is swung in the clockwise direction in FIG. 10 around the shaft 105a against the biasing force of the compression spring 106, so that the hook member 105 and the hook member 105 move. Engagement groove 107
Is disengaged and the lid member 101 of the exposure apparatus is opened.

At the same time, when the display panel 113 receives a display signal from the control unit 110, it displays a message indicating that the biochemical analysis unit 1 and the stimulable phosphor sheet 90 are prohibited from being used together. To do.

Even if the user repeatedly sets the same stimulable phosphor sheet 90 in the exposure device, the lid member 101 is opened each time.

Therefore, the biochemical analysis unit 1 is not allowed to be used together with the user by mistake.
When the stimulable phosphor sheet 90 and the stimulable phosphor sheet 90 are set in the exposure apparatus, the lid member 101 cannot be closed. Therefore, the radioactive label substance selectively contained in the absorptive region 4 of the biochemical analysis unit 1 causes accumulation. The photostimulable phosphor layer region 92 of the luminescent phosphor sheet 90 cannot be exposed.

Furthermore, based on the data input from the reading head 111 of the data reading / recording unit 98,
The stimulable phosphor layer region 92 of the stimulable phosphor sheet 90.
But already M times, for example twice
When it is determined that the exposure is performed, the control unit 110 outputs a drive signal to the solenoid 108 and a display signal to the display panel 113.

As a result, the solenoid 108 is driven and the hook member 105 is swung clockwise around the shaft 105a in FIG. 10 against the urging force of the compression spring 106, and the hook member 105 and the hook member 105 are moved. Engagement groove 107
Is disengaged and the lid member 101 of the exposure apparatus is opened.

At the same time, when the display panel 113 receives a display signal from the control unit 110, it displays a message indicating that the stimulable phosphor sheet 90 cannot be used.

This is because when the photostimulable phosphor layer region 92 of the stimulable phosphor sheet 90 is exposed to the radioactive labeling substance for a predetermined number of times M or more to generate biochemical analysis data, the analysis accuracy is increased. Is significantly reduced, and reliable analysis results cannot be obtained. Where M is
For example, it is set to 2.

Even if the user repeatedly sets the same stimulable phosphor sheet 90 in the exposure apparatus, the lid member 101 is opened each time.

Therefore, the user has M times
When the stimulable phosphor layer region 92 is exposed with a radioactive labeling substance and the stimulable phosphor sheet 90 for which biochemical analysis data has been generated is mistakenly set in the exposure device, the lid member 101 should be closed. Therefore, the stimulable phosphor layer region 92 of the stimulable phosphor sheet 90 cannot be exposed by the radioactive labeling substance selectively contained in the adsorptive region 4 of the biochemical analysis unit 1.

On the other hand, the identification data recorded on the magnetic recording layer 5 of the biochemical analysis unit 1 and the identification data recorded on the magnetic recording layer 94 of the stimulable phosphor sheet 90 have a correspondence relationship, Further, when it is determined that the number of exposures of the stimulable phosphor sheet 90 is less than M times, or the magnetic recording layer 5 of the biochemical analysis unit 1 and the magnetic recording layer 94 of the stimulable phosphor sheet 90 are identified. If no data is recorded and it is determined that the stimulable phosphor sheet 90 has been exposed less than M times, the control unit 11
0 is the magnetic recording head 1 of the data reading / recording unit 98
A write signal is output to 12 to store the stimulable phosphor sheet 9
0 storable phosphor sheet 9 recorded on the magnetic recording layer 94
The number of exposures of 0 is increased by one, and the date and time when the exposure operation is executed is written in the magnetic recording layer 94 based on the built-in clock.

Further, the control unit 110 is
Reading from the magnetic recording layer 5 of the biochemical analysis unit 1,
From the data stored in the memory (not shown),
Data regarding the amount of the adsorptive material contained in the plurality of adsorptive regions 4 of the biochemical analysis unit 1 is read and output to the magnetic recording head 112 of the data reading / recording unit 98, and the stimulable phosphor sheet 90. Of the magnetic recording layer 94.

In these cases, the lid member 10 of the exposure apparatus is used.
Since 1 is locked, a large number of stimulable phosphor layer regions 92 of the stimulable phosphor sheet 90 are produced by the radiolabeling substance selectively contained in the large number of absorptive regions 4 of the biochemical analysis unit 1. Is exposed.

FIG. 12 shows a large number of dot-shaped stimulants formed on the stimulable phosphor sheet 90 by the radioactive labeling substance contained in the large number of absorptive regions 4 formed in the biochemical analysis unit 1. FIG. 9 is a schematic cross-sectional view showing a method of exposing a phosphor layer region 92.

In the exposure apparatus, the stimulable phosphor sheet 90 is superposed on the surface of the biochemical analysis unit 1, and the radiolabeled substance contained in the absorptive region 4 formed in the biochemical analysis unit 1. By the stimulable phosphor sheet 90
The dot-shaped photostimulable phosphor layer region 92 formed in the above is exposed, but in the present embodiment, the biochemical analysis unit 1 has a large number of through holes 3 formed in the aluminum substrate 2. Since it is formed by being filled with nylon 6, it hardly expands or contracts even when subjected to treatment with a liquid such as hybridization.
Each of the absorptive regions 4 formed in the biochemical analysis unit 1 accurately faces the corresponding dot-shaped stimulable phosphor layer region 92 formed in the stimulable phosphor sheet 90, The stimulable phosphor sheet 90 is overlaid on the biochemical analysis unit 1 to form the dot-shaped stimulable phosphor layer region 9
2 can be exposed.

In this way, each of the absorptive regions 4 formed in the biochemical analysis unit 1 over a predetermined period of time has a corresponding dot-shaped stimulable phosphor layer region formed in the stimulable phosphor sheet 90. By stacking the biochemical analysis unit 1 and the stimulable phosphor sheet 90 so as to face 92, the radioactive label substance contained in the adsorptive region 4 formed on the stimulable phosphor sheet 90. A large number of dot-shaped photostimulable phosphor layer regions 92 are exposed.

At this time, an electron beam (β-ray) is emitted from the radioactive labeling substance adsorbed in the absorptive region 4, but the absorptive region 4 of the biochemical analysis unit 1 has a property of attenuating radiation. Since the substrate 2 made of aluminum is formed in a dot shape with being separated from each other, the electron beam (β-ray) emitted from each absorptive region 4 is
The photostimulable phosphor layer scattered in the substrate 2 of the biochemical analysis unit 1 and mixed with the electron beam (β-ray) emitted from the adsorbing regions 4 adjacent to each other and facing the adsorbing regions 4 adjacent to each other. It is possible to effectively prevent the light from entering the region 92, and the dot-shaped stimulable phosphor layer region 92 of the stimulable phosphor sheet 90 has a nickel support having a property of attenuating radiation. Many through holes 1 formed in 91
Since the stimulable phosphor 11 is formed by being embedded in 2, the electron beam (β-ray) emitted from each absorptive region 4 is formed.
It is possible to effectively prevent the light from being scattered in the support 91 of the stimulable phosphor sheet 90 and entering the stimulable phosphor layer region 92 adjacent to the opposing stimulable phosphor layer region 92. Therefore, the electron beam (β-ray) emitted from the radio-labeled substance contained in the adsorptive region 4 can be converted into
An electron beam (β-ray) emitted from the radiolabeled substance contained in the absorptive region 4 can be selectively made incident on the stimulable phosphor layer region 92 facing the absorptive region 4.
Enters the stimulable phosphor layer region 92 to be exposed by the electron beam emitted from the adsorbing regions 4 adjacent to each other,
It is possible to reliably prevent exposure of the stimulable phosphor.

Thus, the radiation data of the radioactive labeling substance is recorded in the large number of dot-shaped stimulable phosphor layer areas 92 formed on the stimulable phosphor sheet 90.

In FIG. 13, the radiation data recorded on the stimulable phosphor sheet 90 is read to generate biochemical analysis data, and at the same time the fluorescence data recorded on the biochemical analysis unit 1 is read to obtain the raw data. FIG. 15 is a schematic perspective view of a scanner that generates data for chemical analysis, and FIG. 14 is a schematic perspective view showing details of the scanner near the photomultiplier.

The scanner according to this embodiment is a unit for radiation data and biochemical analysis of radiolabeled substances recorded in a large number of dot-shaped stimulable phosphor layer regions 92 formed on the stimulable phosphor sheet 90. Fluorescent data such as fluorescent dyes recorded in a large number of absorptive regions 4 can be read.

As shown in FIG. 13, the scanner according to the present embodiment includes a first laser excitation light source 121 which emits a laser beam 124 having a wavelength of 640 nm and a second laser excitation source 121 which emits a laser beam 124 having a wavelength of 532 nm. Excitation light source 12
2 and a third which emits a laser beam 124 having a wavelength of 473 nm
Laser excitation light source 123 of

In this embodiment, the first laser excitation light source 121 is composed of a semiconductor laser light source,
Laser excitation light source 122 and third laser excitation light source 1
23 is the second harmonic generation (Second Harmonic Generatio
n) It is composed of elements.

The laser light 124 generated by the first laser excitation light source 121 is collimated by the collimator lens 125 and then reflected by the mirror 126. In the optical path of the laser light 124 emitted from the first laser excitation light source 121 and reflected by the mirror 126,
A first dichroic mirror 127 that transmits the laser light 4 of 640 nm and reflects light of a wavelength of 532 nm and a second dichroic mirror 128 that transmits light of a wavelength of 532 nm or more and reflects light of a wavelength of 473 nm are provided. The laser light 124 generated by the first laser excitation light source 121 passes through the first dichroic mirror 127 and the second dichroic mirror 128 and enters the mirror 129.

On the other hand, the laser light 124 generated by the second laser excitation light source 122 is collimated by the collimator lens 130 and then reflected by the first dichroic mirror 127 to change its direction by 90 degrees. Then, the light passes through the second dichroic mirror 128 and enters the mirror 129.

The laser light 124 generated from the third laser excitation light source 123 is collimated by the collimator lens 131 and then reflected by the second dichroic mirror 128 to change its direction by 90 degrees. Then, it is incident on the mirror 129.

Laser light 124 incident on the mirror 129
Is reflected by mirror 129, and
It is incident on 32 and is reflected.

In the optical path of the laser beam 124 reflected by the mirror 132, a perforated mirror 134 formed by a concave mirror having a hole 133 formed in the central portion is arranged, and the laser beam reflected by the mirror 132 is arranged. 1
24 passes through the hole 133 of the perforated mirror 134 and enters the concave mirror 138.

Laser light 12 incident on the concave mirror 138
4 is reflected by the concave mirror 138 and enters the optical head 135.

The optical head 135 is provided with a mirror 136 and an aspherical lens 137. The laser light 124 incident on the optical head 135 is reflected by the mirror 136 and the aspherical lens 137 causes the glass plate of the stage 140 to be reflected. Storage phosphor sheet 90 placed on 141
Alternatively, it enters the biochemical analysis unit 1.

The laser light 12 is applied to the stimulable phosphor sheet 90.
4 is incident on the support 91 of the stimulable phosphor sheet 90.
A large number of dot-shaped stimulable phosphor layer regions 92 formed on
When the laser beam 124 is incident on the biochemical analysis unit 1, a fluorescent substance such as a fluorescent dye contained in the many absorptive regions 4 is excited. , Fluorescence 145 is emitted.

Photostimulable light 145 emitted from a large number of dot-shaped photostimulable phosphor layer regions 92 of the stimulable phosphor sheet 90.
Alternatively, many absorptive regions 4 of the biochemical analysis unit 1
The fluorescent light 145 emitted from the laser beam is condensed on the mirror 136 by the aspherical lens 137 provided on the optical head 135, reflected by the mirror 136 on the same side as the optical path of the laser beam 124, and made into parallel light. , Concave mirror 1
It is incident on 38.

Photostimulation 145 incident on the concave mirror 138
Alternatively, the fluorescent light 145 is reflected by the concave mirror 138 and enters the perforated mirror 134.

The photostimulable light 14 incident on the perforated mirror 134.
As shown in FIG. 14, the 5 or the fluorescent light 145 is reflected downward by the perforated mirror 134 formed by the concave mirror and is incident on the filter unit 148, and the light of a predetermined wavelength is cut off. The light enters the multiplier 150 and is photoelectrically detected.

As shown in FIG. 14, the filter unit 148 includes four filter members 151a, 151b,
The filter unit 1 is equipped with 151c and 151d.
The motor 48 is configured to be movable in the left-right direction in FIG. 14 by a motor (not shown).

FIG. 15 is a schematic sectional view taken along the line AA of FIG.

As shown in FIG. 15, the filter member 1
51a includes a filter 152a, and the filter 152a
Is a filter used when exciting the fluorescent substance contained in the large number of absorptive regions 4 formed in the biochemical analysis unit 1 using the first laser excitation light source 121 and reading the fluorescence 145. It is a member, and has a property of cutting light having a wavelength of 640 nm and transmitting light having a wavelength longer than 640 nm.

FIG. 16 is a schematic sectional view taken along the line BB of FIG.

As shown in FIG. 16, the filter member 1
51b includes a filter 152b, and the filter 152b
Is a filter used when exciting the fluorescent substance contained in the large number of absorptive regions 4 formed in the biochemical analysis unit 1 using the second laser excitation light source 122 and reading the fluorescence 145. It is a member and has a property of cutting light having a wavelength of 532 nm and transmitting light having a wavelength longer than 532 nm.

FIG. 17 is a schematic sectional view taken along the line CC of FIG.

As shown in FIG. 17, the filter member 1
51c includes a filter 152c, and the filter 152c
Is used when the fluorescent substance contained in the large number of absorptive regions 4 formed in the biochemical analysis unit 1 is excited by using the third laser excitation light source 123 to read the fluorescence 145. It is a filter member, and has a property of cutting light having a wavelength of 473 nm and transmitting light having a wavelength longer than 473 nm.

FIG. 18 is a schematic sectional view taken along the line DD of FIG.

As shown in FIG. 18, the filter member 1
51d includes a filter 152d, and the filter 152d
Uses the first laser excitation light source 121 to excite a large number of dot-shaped stimulable phosphor layer regions 92 formed on the stimulable phosphor sheet 90, and from the stimulable phosphor layer region 92 It is a filter used when reading the emitted photostimulable light 145, transmits only the light in the wavelength region of the photostimulable light 145 emitted from the photostimulable phosphor layer region 92, and emits light having a wavelength of 640 nm. It has the property of cutting.

Therefore, depending on the laser excitation light source to be used, the filter members 151a, 151b, 151c,
By selectively positioning 151d in front of photomultiplier 150, photomultiplier 15
0 can photoelectrically detect only the light to be detected.

The photomultiplier 150 photoelectrically detects the stimulated emission 145, and the generated analog data is output to the A / D converter 153, digitized, and output to the data processing device 154. It

FIG. 19 is a schematic plan view of the scanning mechanism of the optical head 135. In FIG. 19, for simplification, the optical system except the optical head 135 and the optical paths of the laser light 124 and the fluorescent light 145 or the stimulated emission light 145 are omitted.

As shown in FIG. 19, the optical head 13
The scanning mechanism for scanning 5 includes a substrate 160, and
0 above the sub-scanning pulse motor 161 and the pair of rails 1
62 and 62 are fixed, and further on the substrate 160,
In FIG. 19, a movable substrate 163 is provided in the sub-scanning direction indicated by arrow Y.

A threaded hole (not shown) is formed in the movable substrate 163, and a threaded rod 164 rotated by the sub-scanning pulse motor 161 is formed in this hole. Engaged.

A main scanning stepping motor 165 is provided on the movable substrate 163, and the main scanning stepping motor 165 attaches the endless belt 166 to the adjacent dot-shaped adsorptive properties formed in the biochemical analysis unit 1. Distance between regions 4, that is, stimulable phosphor sheet 90
Adjacent dot-shaped stimulable phosphor layer regions 9 formed in
It can be driven intermittently at a pitch equal to the distance between the two. The optical head 135 is fixed to the endless belt 166, and the main scanning stepping motor 1
When the endless belt 166 is driven by 65, the endless belt 166 is moved in the main scanning direction indicated by an arrow X in FIG. In FIG. 19, 67
Is a linear encoder for detecting the position of the optical head 135 in the main scanning direction, and 168 is a slit of the linear encoder 167.

Therefore, the main scanning stepping motor 1
The endless belt 166 is intermittently driven in the main scanning direction by 65, and when the scanning of one line is completed, the substrate 163 is intermittently moved in the sub scanning direction by the sub scanning pulse motor 161. In FIG. 19, the optical head 135 is moved in the main scanning direction indicated by the arrow X and the sub-scanning direction indicated by the arrow Y, and all the dot shapes formed on the stimulable phosphor sheet 90 by the laser beam 124 are moved. The entire surface of the photostimulable phosphor layer region 92 or the biochemical analysis unit 1 is scanned.

FIG. 20 is a block diagram showing the control system, input system, drive system and detection system of the scanner.

As shown in FIG. 20, the control system of the scanner is a control unit 1 for controlling the entire scanner.
70, and the input system of the scanner is provided with a keyboard 171 that is operated by the user and can input various instruction signals.

As shown in FIG. 20, the drive system of the scanner includes a main scanning stepping motor 165 for intermittently moving the optical head 135 in the main scanning direction, and the optical head 1.
Sub-scanning pulse motor 161 for intermittently moving 35 in the sub-scanning direction, and four filter members 151a, 151.
Filter unit 14 including b, 151c and 151d
8 is provided with a filter unit motor 172.

The control unit 170 selectively outputs a drive signal to the first laser pumping light source 121, the second laser pumping light source 122 or the third laser pumping light source 123, and also outputs a drive signal to the filter unit motor 172. It is configured to output.

As shown in FIG. 20, the detection system of the scanner includes a photomultiplier 150, a linear encoder 167 for detecting the position of the optical head 135 in the main scanning direction, and a magnetic field of the biochemical analysis unit 1. A reader 173 for reading the data recorded in the recording layer 5 and the magnetic recording layer 94 of the stimulable phosphor sheet 90 is provided.

In the present embodiment, the control unit 170 controls the first laser pumping light source 121, the second laser pumping light source 122 or the third laser pumping light source 121 according to the position detection signal of the optical head 135 input from the linear encoder 167. The laser excitation light source 123 is configured to be turned on / off.

The scanner according to the present embodiment configured as described above records in a large number of dot-shaped stimulable phosphor layer areas 92 formed on the stimulable phosphor sheet 90 as follows. The radiation data obtained is read and biochemical analysis data is generated.

First, the stimulable phosphor sheet 90 is placed on the glass plate 141 of the stage 140.

[0387] Next, by the user, the keyboard 1
An instruction signal for scanning a large number of dot-shaped stimulable phosphor layer areas 92 formed on the stimulable phosphor sheet 90 with the laser light 124 is input to 71.

The instruction signal input to the keyboard 171 is input to the control unit 170, and the control unit 170 outputs a drive signal to the filter unit motor 172 in accordance with the instruction signal to move the filter unit 148 to perform the photostimulation. 640 nm, which transmits only the light in the wavelength range of the photostimulable light 145 emitted from the fluorescent substance
152d having a property of cutting off light of wavelength
The filter member 151d provided with is positioned in the optical path of the stimulated emission 145.

On the other hand, when the stimulable phosphor sheet 90 is placed on the glass plate 141 of the stage 140, the magnetic recording layer 94 of the stimulable phosphor sheet 90 is read by the reader 173 provided above the stage 140. The data relating to the amounts of the adsorptive material contained in the plurality of adsorptive regions 4 of the biochemical analysis unit 1 recorded in step 1 are read and output to the control unit 170. The control unit 170 stores the input data in a memory (not shown).

Further, the control unit 170 is
The drive signal is output to the main scanning stepping motor 165,
The optical head 135 is moved in the main scanning direction, and the first dot-shaped photostimulable phosphor layer region 92 is irradiated with the laser beam 124 based on the position detection signal of the optical head 135 input from the linear encoder 167. When it is confirmed that the optical head 135 has moved to a possible position, a stop signal is output to the main scanning stepping motor 165, and
A drive signal is output to the first laser excitation light source 121,
The first laser excitation light source 121 is activated to emit the laser light 124 having a wavelength of 640 nm.

The laser light 124 emitted from the first laser excitation light source 121 is made into parallel light by the collimator lens 125, and then enters the mirror 126 and is reflected.

The laser beam 124 reflected by the mirror 126 passes through the first dichroic mirror 127 and the second dichroic mirror 128, and the mirror 12
It is incident on 9.

Laser light 124 incident on the mirror 129
Is reflected by the mirror 129, and then enters the mirror 132 and is reflected.

The laser beam 124 reflected by the mirror 132 passes through the hole 133 of the perforated mirror 134,
It is incident on the concave mirror 138.

Laser light 12 incident on the concave mirror 138
4 is reflected by the concave mirror 138 and enters the optical head 135.

Laser light 12 incident on the optical head 135
4 is reflected by the mirror 136, and the aspherical lens 1
By 37, the light is focused on the first dot-shaped stimulable phosphor layer region 92 of the stimulable phosphor sheet 90 placed on the stage 140 glass plate 141.

As a result, the stimulable phosphor contained in the first dot-shaped stimulable phosphor layer area 92 formed on the stimulable phosphor sheet 90 is excited by the laser beam 124 to generate the first stimulable phosphor. Photostimulation light 145 is emitted from the photostimulable phosphor layer region 92 of FIG.

At this time, since the support 91 of the stimulable phosphor sheet 90 is made of nickel, the laser light 124 is scattered inside the support 91 and the first stimulable phosphor layer region 92 is formed. The stimulable phosphor contained in the adjacent stimulable phosphor layer region 92 is excited to effectively prevent the accumulated radiation energy from being emitted in the form of stimulable light 145. In addition, the stimulable light 145 emitted from the first stimulable phosphor layer region 92 is converted into the support 9
It is possible to effectively prevent scattering within 1 and not being detected by the photomultiplier 150.

The photostimulable light 145 emitted from the first dot-shaped photostimulable phosphor region 12 is collected by the aspherical lens 137 provided in the optical head 135, and the mirror 1
The light is reflected on the same side as the optical path of the laser light 124 by the light beam 36, becomes parallel light, and is incident on the concave mirror 138.

The photostimulable light 145 incident on the concave mirror 138.
Is reflected by the concave mirror 138 and enters the perforated mirror 134.

The photostimulable light 14 incident on the perforated mirror 134.
5 is a perforated mirror 13 formed by a concave mirror
As shown in FIG. 14, the light is reflected downward by the light source 4 and enters the filter 152d of the filter unit 148.

The filter 152d transmits only the light in the wavelength region of the stimulable light 145 emitted from the stimulable phosphor,
Since it has the property of cutting light with a wavelength of 0 nm,
The photostimulable light 1 emitted from the dot-shaped photostimulable phosphor layer region 92 in which the light having a wavelength of 640 nm which is the excitation light is cut off.
Only light in the 45 wavelength range passes through the filter 152d,
It is detected photoelectrically by the photomultiplier 150.

The analog data generated by being photoelectrically detected by the photomultiplier 150 is A / D.
It is digitized by the converter 153 and output to the data processing device 154.

After the first laser excitation light source 121 is turned on, when a predetermined time, for example, several microseconds has elapsed, the control unit 170 causes the first laser excitation light source 12 to turn on.
1 outputs a drive stop signal to the first laser excitation light source 1
21 is stopped, and a drive signal is output to the main scanning stepping motor 165 to output the optical head 13
5 is moved by a pitch equal to the distance between adjacent dot-shaped stimulable phosphor layer regions 92 formed on the stimulable phosphor sheet 90.

The data processing device 154 photoelectrically detects the photostimulable light 145 emitted from the first photostimulable phosphor layer region 92, and when the digital data generated by digitization is input, it is stored in the memory. Data relating to the amount of the adsorbent material contained in the plurality of adsorbent regions 4 of the biochemical analysis unit 1 stored in (not shown) is read out, the digital data is corrected, and the first photostimulation is performed. Fluorescent phosphor layer region 9
Data for biochemical analysis corresponding to the radiation data recorded in 2 is generated. This makes it possible to prevent noise due to variations in the amount of the adsorptive material contained in each adsorptive region 4 from being generated in the biochemical analysis data.

On the basis of the position detection signal of the optical head 135 input from the linear encoder 167, the optical head 135 causes the adjacent dot-shaped stimulable phosphor layer regions 92 to move.
The second dot-shaped stimulable phosphor layer region formed on the stimulable phosphor sheet 90 is formed by moving the laser light 124 emitted from the first laser excitation light source 121 by one pitch equal to the distance between them. When it is confirmed that the control unit 170 has moved to a position where irradiation can be performed on 92, the control unit 170
Of the second dot-shaped stimulable fluorescent light formed on the stimulable phosphor sheet 90 by the laser light 124 by outputting a drive signal to the laser excitation light source 121 of FIG. The stimulable phosphor contained in the body layer region 92 is excited.

Similarly, the laser light 124 emitted from the first laser pumping light source 121 for a predetermined time.
Is irradiated to the second dot-shaped stimulable phosphor layer area 92 formed on the stimulable phosphor sheet 90, and the stimulable fluorescence contained in the second stimulable phosphor layer area 92 is The photomultiplier 150 causes the photostimulable light 145 emitted from the second photostimulable phosphor layer region 92 to be excited by the photomultiplier 150.
Photoelectrically detected, analog data is generated, A /
When biochemical analysis data is generated from the radiation data digitized by the D converter 153 and recorded in the second stimulable phosphor layer region 92, the control unit 170 causes the first laser An off signal is output to the excitation light source 121 to turn off the first laser excitation light source 121, and a drive signal is output to the main scanning stepping motor 165 to cause the optical heads 135 to have adjacent dot-shaped photostimulability. It is moved by one pitch equal to the distance between the phosphor layer regions 92.

The data processing device 154 photoelectrically detects the photostimulable light 145 emitted from the second photostimulable phosphor layer region 92, and when digital data generated by digitization is input, the memory is stored. The data concerning the amount of the adsorbent material contained in the plurality of adsorbent regions 4 of the biochemical analysis unit 1 stored in (not shown) is read out, the digital data is corrected, and the second photostimulation is performed. Fluorescent phosphor layer region 9
Data for biochemical analysis corresponding to the radiation data recorded in 2 is generated. This makes it possible to prevent noise due to variations in the amount of the adsorptive material contained in each adsorptive region 4 from being generated in the biochemical analysis data.

In this way, the first laser excitation light source 121 is repeatedly turned on and off in synchronization with the intermittent movement of the optical head 135, and the optical head 135 is detected based on the position detection signal input from the linear encoder 167. 1
35 is moved by one line in the main scanning direction,
When it is confirmed that the scanning of the dot-shaped stimulable phosphor layer area 92 of the line by the laser light 124 is completed, the control unit 170 outputs a drive signal to the main scanning stepping motor 165 to cause the optical head. 135 is returned to the original position and the sub-scanning pulse motor 16
A drive signal is output to 1 to move the movable substrate 163 in the sub-scanning direction by one line.

The optical head 135 is returned to its original position based on the position detection signal of the optical head 135 input from the linear encoder 167, and the movable substrate 1 is moved.
When it is confirmed that 63 has been moved by one line in the sub-scanning direction, the control unit 170 causes the dot-shaped stimulable phosphor layer region 92 on the first line to sequentially move to the first line. In the same manner as when the laser light 124 emitted from the laser excitation light source 121 is irradiated, the emission from the first laser excitation light source 121 is sequentially applied to the dot-shaped stimulable phosphor layer region 92 of the second line. The stimulable phosphor contained in the dot-shaped stimulable phosphor layer region 92 is excited by irradiating the laser beam 124 to generate the stimulable phosphor layer region 15
The photomultipliers 150 sequentially detect the photostimulated stimuli 145 emitted from the photomultipliers.

The analog data generated photoelectrically detected by the photomultiplier 150 is converted into analog data.
Biochemical analysis data is generated from the radiation data output to the converter 153, digitized, and recorded in each dot-shaped stimulable phosphor layer region 92.

The data processing device 154 photoelectrically detects the photostimulable light 145 emitted from each photostimulable phosphor layer region 92, and when digital data generated by digitization is input, it is stored in a memory (Fig. Data regarding the amount of the adsorptive material contained in the plurality of adsorptive regions 4 of the biochemical analysis unit 1 stored in (not shown) is read out, the digital data is corrected, and each stimulable phosphor layer is read. Biochemical analysis data corresponding to the radiation data recorded in the area 92 is generated. This makes it possible to prevent noise due to variations in the amount of the adsorptive material contained in each adsorptive region 4 from being generated in the biochemical analysis data.

In this way, the large number of dot-shaped stimulable phosphor layer areas 92 formed on the stimulable phosphor sheet 90 are all scanned by the laser light 124 emitted from the first laser excitation light source 121, and a large number of them are scanned. The stimulable phosphor contained in the dot-shaped stimulable phosphor layer region 92 is excited, and emitted stimulable light 145 is photoelectrically detected by the photomultiplier 150 and generated. When the analog data is digitized by the A / D converter 153 and the biochemical analysis data is generated from the radiation data recorded in each dot-shaped stimulable phosphor layer region 92, the control unit 170 is generated. Then, the drive stop signal is output to the first laser excitation light source 121, and the drive of the first laser excitation light source 121 is stopped.

On the other hand, when the fluorescence data of the fluorescent substance recorded in the many absorptive regions 4 formed in the biochemical analysis unit 1 is read and the digital data for biochemical analysis is generated, first, by the user. The biochemical analysis unit 1 is set on the glass plate 141 of the stage 140.

Next, the keyboard 1 is selected by the user.
At 71, the type of fluorescent substance that is a labeling substance is specified, and an instruction signal to the effect that fluorescence data should be read is input.

The instruction signal input to the keyboard 171 is input to the control unit 170, and when the control unit 170 receives the instruction signal, it follows the table stored in the memory (not shown).
The laser excitation light source to be used is determined, and which of the filters 152a, 152b, 152c, 152d is to be positioned in the optical path of the fluorescent light 145.

For example, Rhodamine (registered trademark) which can be most efficiently excited by a laser having a wavelength of 532 nm as a fluorescent substance for labeling a substance of biological origin
Is used and the fact is input to the keyboard 171, the control unit 170 selects the second laser excitation light source 122 and the filter 152b.
Is selected, a drive signal is output to the filter unit motor 172 to move the filter unit 148, and 532
A filter member 151b provided with a filter 152b having a property of cutting light having a wavelength of nm and transmitting light having a wavelength longer than 532 nm is located in the optical path of the fluorescence 145 to be emitted from the biochemical analysis unit 1. Let

On the other hand, when the biochemical analysis unit 1 is placed on the glass plate 141 of the stage 140, the magnetic recording layer 5 of the biochemical analysis unit 1 is read by the reader 173 provided above the stage 140. The data relating to the amounts of the adsorptive material contained in the plurality of adsorptive regions 4 of the biochemical analysis unit 1 recorded in step 1 are read and output to the control unit 170. The control unit 170 stores the input data in a memory (not shown).
To memorize.

Further, the control unit 170 is
The drive signal is output to the main scanning stepping motor 165,
The optical head 135 is moved in the main scanning direction, and based on the position detection signal of the optical head 135 input from the linear encoder, the first of the many absorptive regions 4 formed in the biochemical analysis unit 1 is detected. When it is confirmed that the optical head 135 has reached the position where the laser beam 124 can be irradiated to the absorptive region 4, the main scanning stepping motor 16
5 outputs a stop signal to the second laser pumping light source 122 and outputs a driving signal to the second laser pumping light source 122 to activate the second laser pumping light source 122, and the laser light 124 having a wavelength of 532 nm
To emit.

The laser light 124 emitted from the second laser excitation light source 122 is made into parallel light by the collimator lens 130, then enters the first dichroic mirror 127 and is reflected.

The laser beam 124 reflected by the first dichroic mirror 127 passes through the second dichroic mirror 128 and enters the mirror 129.

Laser light 124 incident on the mirror 129
Is reflected by the mirror 129, and then enters the mirror 132 and is reflected.

The laser beam 124 reflected by the mirror 132 passes through the hole 133 of the perforated mirror 134,
It is incident on the concave mirror 138.

Laser light 12 incident on the concave mirror 138
4 is reflected by the concave mirror 138 and enters the optical head 135.

Laser light 12 incident on the optical head 135
4 is reflected by the mirror 136, and the aspherical lens 1
By 37, the light is focused on the biochemical analysis unit 1 placed on the glass plate 141 of the stage 140.

As a result, the laser beam 124 excites a fluorescent substance such as a fluorescent dye contained in the first adsorptive region 4 of the biochemical analysis unit 1, for example, rhodamine to emit fluorescence.

Here, in the biochemical analysis unit 1 according to this embodiment, the absorptive regions 4 are adsorbed in a large number of through holes 3 formed on the aluminum substrate 2 so as to be separated from each other. The fluorescent substance contained in the absorptive region 4 is formed by filling the absorptive region 4 with the substrate 2 made of aluminum having a property of attenuating light around the absorptive region 4. It is possible to reliably prevent the fluorescence 145 emitted from the fluorescent substance 145 from being mixed with the fluorescence 145 emitted from the fluorescent substance contained in the adsorbing regions 4 adjacent to each other.

The fluorescence 145 emitted from rhodamine is
The laser beam 124 is condensed by the aspherical lens 137 provided in the optical head 135 and is reflected by the mirror 136.
Is reflected to the same side as the optical path of, and is made into parallel light, and is incident on the concave mirror 138.

Fluorescent light 145 incident on the concave mirror 138
Is reflected by the concave mirror 138 and enters the perforated mirror 134.

Fluorescent light 145 incident on the perforated mirror 134
Is a perforated mirror 134 formed by a concave mirror
Is reflected downwards as shown in FIG.
The light enters the filter 152b of the filter unit 148.

Since the filter 152b has a property of cutting light having a wavelength of 532 nm and transmitting light having a wavelength longer than 532 nm, light having a wavelength of 532 nm, which is excitation light, is cut and emitted from rhodamine. Fluorescence 1
Only light in the 45 wavelength range passes through the filter 152b,
It is detected photoelectrically by the photomultiplier 150.

The analog signal photoelectrically detected and generated by the photomultiplier 150 is output to the A / D converter 153 and converted into a digital signal.
It is output to the data processing device 154.

The data processing device 154 photoelectrically detects the fluorescence 145 emitted from the first absorptive region 4 and, when digital data generated by digitization is input, is stored in a memory (not shown). The stored data regarding the amount of the adsorptive material contained in the plurality of adsorptive regions 4 of the biochemical analysis unit 1 is read out, the digital data is corrected, and the data is recorded in the first adsorptive region 4. Generates biochemical analysis data corresponding to the existing fluorescence data. This makes it possible to prevent noise due to variations in the amount of the adsorptive material contained in each adsorptive region 4 from being generated in the biochemical analysis data.

After the second laser excitation light source 122 is turned on, when a predetermined time, for example, several microseconds has elapsed, the control unit 170 causes the second laser excitation light source 12
2 outputs a drive stop signal to the second laser excitation light source 1
22 is stopped and a drive signal is output to the main scanning stepping motor 165 to output the optical head 13
5 is moved by a pitch equal to the distance between the absorptive regions 4 formed in the biochemical analysis unit 1.

Based on the position detection signal of the optical head 135 input from the linear encoder 167, the optical head 135 is moved by one pitch equal to the distance between the adsorbing regions 4 adjacent to each other formed in the biochemical analysis unit 1. The laser beam 124 that is moved and emitted from the second laser excitation light source 122 is converted into the second laser beam that is formed in the biochemical analysis unit 1.
When it is confirmed that the laser beam has been moved to a position where it can be irradiated to the absorptive region 4, the control unit 170 outputs a drive signal to the second laser excitation light source 122 to turn on the second laser excitation light source 122. Then, the laser light 124 excites the fluorescent substance, for example, rhodamine, contained in the second absorptive region 4 formed in the biochemical analysis unit 1.

[0436] Similarly, the laser light 124 is emitted from the second beam formed on the biochemical analysis unit 1 for a predetermined time.
When the fluorescent light 145 emitted from the second absorptive region 4 of the second adsorption region 4 is photoelectrically detected by the photomultiplier 150 and analog data is generated, the control unit 170 causes the An off signal is output to the second laser excitation light source 122 to turn off the second laser excitation light source 122, and a drive signal is output to the main scanning stepping motor 165 to output the optical head 13
5 is moved by one pitch equal to the distance between the adsorbing regions 4 adjacent to each other formed in the biochemical analysis unit 1.

In this way, the first laser excitation light source 121 is repeatedly turned on and off in synchronization with the intermittent movement of the optical head 135, and the optical head 135 is detected based on the position detection signal input from the linear encoder 167. 1
35 is moved by one line in the main scanning direction, and when it is confirmed that all the absorptive regions 4 on the first line of the biochemical analysis unit 1 are scanned by the laser beam 124, control is performed. The unit 170 outputs a drive signal to the main scanning stepping motor 165 to return the optical head 135 to the original position, and outputs a drive signal to the sub scanning pulse motor 161 to move the movable substrate 1.
63 is moved by one line in the sub-scanning direction.

The optical head 135 is returned to its original position based on the position detection signal of the optical head 135 input from the linear encoder 167, and the movable substrate 1 is moved.
When it is confirmed that 63 has been moved by one line in the sub-scanning direction, the control unit 170 sequentially advances to the absorptive region 4 of the first line formed in the biochemical analysis unit 1. , The second line absorptive region 4 and the second line absorptive property formed in the biochemical analysis unit 1 in exactly the same manner as the irradiation of the laser beam 124 emitted from the second laser excitation light source 122. Rhodamine contained in the region 4 is excited, and the fluorescence 145 emitted from the adsorptive region 4 is sequentially detected photoelectrically by the photomultiplier 150.

The analog data generated photoelectrically detected by the photomultiplier 150 is converted into analog data.
It is converted into digital data by the converter 153 and sent to the data processing device 154.

The data processing device 154 is arranged so that each absorptive area 4
When the digital data generated by photoelectrically detecting the fluorescence 145 emitted from the device and digitizing it is input,
Data relating to the amount of the adsorptive material contained in the plurality of adsorptive regions 4 of the biochemical analysis unit 1 stored in the memory (not shown) is read out, the digital data is corrected, and each adsorptive region is corrected. Data for biochemical analysis corresponding to the fluorescence data recorded in 4 is generated. This makes it possible to prevent noise due to variations in the amount of the adsorptive material contained in each adsorptive region 4 from being generated in the biochemical analysis data.

In this way, the entire surface of the biochemical analysis unit 1 is scanned by the laser beam 124 emitted from the second laser excitation light source 122, and a large number of absorptive regions 4 formed in the biochemical analysis unit 1 are formed. Rhodamine contained therein is excited, the emitted fluorescence 145 is photoelectrically detected by the photomultiplier 150, and the generated analog data is converted into digital data by the A / D converter 153, When sent to the data processing device 154, the drive stop signal is output from the control unit 170 to the second laser excitation light source 122, and the drive of the second laser excitation light source 122 is stopped.

As described above, the biochemical analysis data is generated based on the radiation data and the fluorescence data recorded in the absorptive region 4 of the biochemical analysis unit 1.

The specific binding substance adsorbed on the absorptive region 4 is separated from the substance derived from the living body labeled with the labeling substance by N
Biochemical analysis units 1 and M hybridized and used to generate biochemical analysis data for a number of times
The absorptive region 4 of the biochemical analysis unit 1
The stimulable phosphor sheet 90 that has been exposed by the radioactive labeling substance selectively contained in the above and used for the generation of the biochemical analysis data is sent to the manufacturer for recycling.

When the biochemical analysis unit 1 is received from the user for recycling, the manufacturer reads the data recorded on the magnetic recording layer 5 of the biochemical analysis unit 1 with a reader (not shown), Magnetic recording layer 5
When the number of times of recycling recorded in the first magnetic recording area 5a is less than the predetermined number of times, the type of the labeling substance used is determined, and when the radioactive labeling substance is not used as the labeling substance, The adsorptive region 4 of the biochemical analysis unit 1 is washed to remove the adsorbed specific binding substance, and then the spotting device is used to newly drop the specific binding substance onto the adsorption region 4. The biochemical analysis unit is shipped after increasing the number of recycles recorded in the first magnetic recording area 5a of the magnetic recording layer 5 by one.

[0445] On the other hand, when a radioactive labeling substance is used as the labeling substance, the manufacturer is not allowed to use the adsorption substance filled in the large number of through holes 3 formed in the substrate 2 of the biochemical analysis unit 1. Of the magnetic recording layer after removing the absorptive material and filling a new absorptive material to regenerate the absorptive region 4 and newly dropping a specific binding substance into the absorptive region 4 using a spotting device. The biochemical analysis unit is shipped after increasing the number of times of recycle recorded in the first magnetic recording area 5a of No. 5 by one. The adsorptive material removed from the through hole 3 of the biochemical analysis unit 1 is recorded in the first magnetic recording area 5a of the magnetic recording layer 5 of the biochemical analysis unit 1 It will be managed until it can be disposed of.

On the other hand, when receiving the stimulable phosphor sheet 90 from the user for recycling, the manufacturer
The stimulable phosphor filled in the through hole 93 of the stimulable phosphor sheet 90 is removed, and a new stimulable phosphor is filled,
The stimulable phosphor layer region 92 is reproduced, and the number of recycles recorded in the magnetic recording layer 94 of the stimulable phosphor sheet 90 is set to 1
The stimulable phosphor sheet is shipped after increasing the number of times. The stimulable phosphor removed from the through hole 93 of the stimulable phosphor sheet 90 is irradiated with light to erase the accumulated radiation energy, and then discarded.

According to this embodiment, the magnetic recording layer 5 is formed on the substrate 2 of the biochemical analysis unit 1, and the magnetic recording layer 5 is formed on the first magnetic recording area 5a in which data cannot be written by the user. Since the data regarding the type of the specific binding substance dropped and the position of the absorptive region 4 dropped is recorded, the user can select the first magnetic recording region of the magnetic recording layer 5 of the biochemical analysis unit 1. By reading the data recorded in 5a using a reader (not shown), the specific binding substance adsorbed in the absorptive region 4 of the biochemical analysis unit 1 can be surely added to the desired living body. It becomes possible to hybridize the substance of origin and generate the data for biochemical analysis, and it is possible to greatly improve the efficiency and accuracy of biochemical analysis.

Further, according to this embodiment, the magnetic recording layer 5 is formed on the substrate 2 of the biochemical analysis unit 1, and the first magnetic recording region in which data cannot be written by the user of the magnetic recording layer 5 is formed. Since the number of times of use of the biochemical analysis unit 1 is recorded in 5a, by managing the data recorded in the first magnetic recording area 5a of the magnetic recording layer 5, it is used over a predetermined number of times and the adsorptivity is improved. By using the biochemical analysis unit 1 in which a part of the specific binding substance adsorbed in the region 4 is peeled off, it is possible to reliably prevent the user from performing the biochemical analysis, and thus the biochemical analysis is performed. It is possible to greatly improve the efficiency and accuracy of analysis.

Furthermore, according to the present embodiment, the hybridization apparatus is equipped with the read head 37 for reading the data recorded in the first magnetic recording area 5a of the magnetic recording layer 5 of the biochemical analysis unit 1, and the reading head 37 is provided. Head 3
When it is determined that the biochemical analysis unit 1 has been used N times based on the data read by the control unit 7, the control unit 60 sends the biochemical analysis unit 1 back to the user to perform hybridization. Since the biochemical analysis unit 1 is configured so that it cannot be executed, a part of the specific binding substance used and adsorbed in the absorptive region 4 is peeled off for a predetermined number of times, It can reliably prevent the user from performing biochemical analysis and thus
It is possible to greatly improve the efficiency and accuracy of biochemical analysis.

Further, in this embodiment, the absorptive material is filled in the large number of through holes 3 formed in the substrate 2 of the biochemical analysis unit 1 to form the absorptive region 4, and all the absorptive regions 4 are formed. It is difficult to form the through holes 3 to have a uniform size, and it is also difficult to uniformly fill the through holes 3 with the adsorbent material. Therefore, the biochemical analysis unit 1 has the adsorbability. The radiation data recorded in the area 4 is transferred to the stimulable phosphor layer area 92 of the stimulable phosphor sheet 90, and the stimulable phosphor layer area 92 is irradiated with the laser beam 124 to emit the stimulable fluorescence. The biochemical analysis unit 1 is included in the biochemical analysis data obtained by exciting the photostimulable phosphor contained in the body layer region 92 and photoelectrically detecting the emitted photostimulable light 145.
Noise may be generated due to the non-uniform amount of the absorptive material contained in the absorptive region 4 of
According to this embodiment, the data regarding the amount of the absorptive material contained in the plurality of absorptive regions 4 recorded in the magnetic recording layer 5 of the biochemical analysis unit 1 is read by the read head 111 of the exposure apparatus. And is written in the magnetic recording layer 94 of the stimulable phosphor sheet 90, and included in the plurality of absorptive regions 4 recorded in the magnetic recording layer 94 of the stimulable phosphor sheet 90 when the biochemical analysis data is generated. The digital data obtained by reading the data regarding the amount of the adsorbent material present and photoelectrically detecting the stimulated emission 145 based on the data regarding the amount of the adsorbent material contained in the plurality of adsorbent regions 4. Since the data is corrected and the biochemical analysis data is generated, noise caused by the non-uniform amount of the adsorptive material contained in the adsorptive region 4 of the biochemical analysis unit 1 is generated. It becomes possible to effectively prevent the produced during chemical analysis data.

Further, in the present embodiment, the absorptive material is filled in the large number of through holes 3 formed in the substrate 2 of the biochemical analysis unit 1 to form the absorptive region 4, and all of the absorptive regions 4 are formed. It is difficult to form the through holes 3 to have a uniform size, and it is also difficult to uniformly fill the through holes 3 with the adsorbent material. Therefore, the biochemical analysis unit 1 has the adsorbability. Fluorescence data is recorded in the region 4, and the absorptive region 4 of the biochemical analysis unit 1 is irradiated with the laser beam 124 to excite the fluorescent substance and photoelectrically detect the emitted fluorescence 145 to obtain. In the biochemical analysis data, noise may be generated due to the non-uniform amount of the adsorptive material contained in the adsorptive region 4 of the biochemical analysis unit 1. According to, it is contained in the plurality of absorptive regions 4. Data on the amount of wear resistant material,
It is recorded in the magnetic recording layer 5 of the biochemical analysis unit 1 and is included in the plurality of absorptive regions 4 recorded in the magnetic recording layer 5 of the biochemical analysis unit 1 when the biochemical analysis data is generated. The data regarding the amount of the adsorbent material is read, and the digital data obtained by photoelectrically detecting the fluorescence 145 is corrected based on the data regarding the amount of the adsorbent material included in the plurality of adsorbent regions 4. , Because the biochemical analysis data has been generated, the biochemical analysis unit 1
It is possible to effectively prevent generation of noise in the biochemical analysis data due to the non-uniform amount of the adsorbent material contained in the adsorbent region 4.

In addition, according to this embodiment, since the user can use the specific biochemical analysis unit 1 and the specific stimulable phosphor sheet 90 together,
When the specific biochemical analysis unit 1 and the specific storage phosphor sheet 90 are provided to the user as one biochemical analysis kit, the magnetic recording layer 5 and the storage of the biochemical analysis unit 1 are stored. Corresponding identification data is recorded on the magnetic recording layer 94 of the luminescent phosphor sheet 90, so that only the specific biochemical analysis unit 1 and the specific stimulable phosphor sheet 90 are used together. Since it is guaranteed, the labeled substance derived from the living body is selectively adsorbed to the absorptive region 4 of the biochemical analysis unit 1 by a radiolabeled substance, which is collected from a person whose gene expression abnormality is recognized. The stimulable phosphor layer region 92 of the stimulable phosphor sheet 90 is specifically bound to the specific binding substance and selectively contained in the adsorptive region 4 by the radioactive labeling substance.
The obtained data for biochemical analysis and the substance derived from the living body, which is collected from a healthy person and labeled with a radioactive labeling substance, are selectively adsorbed in the adsorption region 4 of the biochemical analysis unit 1. The stimulable phosphor layer region 92 of the stimulable phosphor sheet 90 is exposed by the radioactive labeling substance selectively contained in the adsorptive region 4 by specifically binding to the specific binding substance adsorbed on the. When comparing the obtained biochemical analysis data and performing the test, the test is always performed using the same biochemical analysis unit 1 and the stimulable phosphor sheet 90. It is possible to greatly improve the inspection accuracy.

Furthermore, according to this embodiment, the specific biochemical analysis unit 1 and the specific stimulable phosphor sheet 90 are used.
In order to allow the user to use both and, the specific biochemical analysis unit 1 and the specific stimulable phosphor sheet 90 are combined into one biochemical analysis kit,
When provided to the user, the identification data recorded on the magnetic recording layer 5 of the biochemical analysis unit 1 and the identification recorded on the magnetic recording layer 94 of the stimulable phosphor sheet 90 are read by the reading head 111 of the exposure apparatus. When the data is read and the identification data does not correspond, the biochemical analysis unit 1 is used so that the stimulable phosphor sheet 90 cannot be exposed. A substance derived from a living body, which is collected from an authorized person and is labeled with a radiolabeled substance, is selectively
The radiolabeling substance selectively bound to the specific binding substance adsorbed in the adsorptive region 4 of the biochemical analysis unit 1 and selectively contained in the adsorptive region 4 makes the stimulable phosphor sheet 90 bright. The biodegradable phosphor layer region 92 is exposed to light, and the obtained biochemical analysis data and a substance derived from a living body which is collected from a healthy subject and is labeled with a radiolabeled substance are selectively used for biochemical analysis. The specific binding substance adsorbed on the adsorptive region 4 of the unit 1 is specifically bound to
The stimulable phosphor layer region 92 of the stimulable phosphor sheet 90 is exposed by the radioactive labeling substance selectively contained in
When comparing the obtained biochemical analysis data and performing the test, the same biochemical analysis unit 1 is always used,
An inspection is performed using the stimulable phosphor sheet 90,
Therefore, the inspection accuracy can be significantly improved.

Further, according to this embodiment, during the hybridization, the date and time when the hybridization was performed and whether or not the radiolabeled substance was used are recorded in the magnetic recording layer 5 of the biochemical analysis unit 1. , When recycling the biochemical analysis unit 1,
Appropriate processing can be performed according to the usage history of the biochemical analysis unit 1, and when a radiolabeled substance is used, the adsorptive material removed from the adsorptive region 4 is appropriately managed. However, it becomes possible to dispose of it at an appropriate time.

Further, according to this embodiment, the number of times of recycling is recorded in the magnetic recording layer 5 of the biochemical analysis unit 1 and the magnetic recording layer 94 of the stimulable phosphor sheet 90. From biochemical analysis unit 1
Substrate 2 and support 91 for stimulable phosphor sheet 90
Can be used efficiently and resource saving can be achieved.

According to this embodiment, the magnetic recording layer 5 of the biochemical analysis unit 1 is provided with the second magnetic recording area 5b in which the user can write data. Manages the biochemical analysis unit 1, such as data identifying a person who has collected a substance of biological origin to be selectively hybridized to the specific binding substance adsorbed in the adsorptive region 4 of the chemical analysis unit 1. In the above, personally necessary data can be written and recorded, and the efficiency of biochemical analysis can be greatly improved.

Furthermore, according to this embodiment, the specific binding substance adsorbed in the absorptive region 4 of the biochemical analysis unit 1 is labeled with a radiolabeling substance from a living body using a hybridization apparatus. The substances of biological origin that are labeled with the substance and the fluorescent substance are selectively hybridized, so that it is possible to significantly reduce the labor, and the hybridization result varies depending on the user who executes the hybridization. It is possible to minimize the occurrence.

Further, according to this embodiment, the absorptive region 4 of the biochemical analysis unit 1 is formed in a dot shape on the substrate 2 formed of aluminum having a property of attenuating radiation so as to be separated from each other. Therefore, the electron beam (β-ray) emitted from each absorptive region 4 at the time of exposure
Are scattered in the substrate 2 of the biochemical analysis unit 1 and are mixed with the electron beam (β-ray) emitted from the adsorbing regions 4 adjacent to each other, and the stimulable fluorescence that opposes the adsorbing regions 4 adjacent to each other. It is possible to effectively prevent the light from entering the body layer region 92, and the dot-shaped stimulable phosphor layer region 92 of the stimulable phosphor sheet 90 is made of nickel having a property of attenuating radiation. Since the stimulable phosphor is embedded in a large number of through holes 93 formed in the support 91, the electron beam (β-ray) emitted from each absorptive region 4 is formed.
It is possible to effectively prevent the light from being scattered in the support 91 of the stimulable phosphor sheet 90 and entering the stimulable phosphor layer region 92 adjacent to the opposing stimulable phosphor layer region 92. Therefore, the electron beam (β-ray) emitted from the radio-labeled substance contained in the adsorptive region 4 can be converted into
An electron beam (β-ray) emitted from the radiolabeled substance contained in the absorptive region 4 can be selectively made incident on the stimulable phosphor layer region 92 facing the absorptive region 4.
Enters the stimulable phosphor layer region 92 to be exposed by the electron beam emitted from the adsorbing regions 4 adjacent to each other,
Since it is possible to reliably prevent the stimulable phosphor from being exposed, the regions 92 of the stimulable phosphor layer to be exposed by the radioactive labeling substance contained in each of the absorptive regions 4 are adjacent to each other. The electron beam (β-ray) emitted from the radio-labeled substance contained in the biogenic region 4 can prevent noise due to exposure from being generated in the biochemical analysis data, It becomes possible to greatly improve the quantitativeness of biochemical analysis.

FIG. 21 is a schematic perspective view of a biochemical analysis unit according to another preferred embodiment of the present invention.

As shown in FIG. 21, the biochemical analysis unit 180 according to this embodiment is made of aluminum in which a large number of substantially circular through holes 183 are formed in a dot shape in accordance with a regular pattern. The substrate 181 and the adsorptive film 182 made of nylon 6 are provided, and the adsorptive film 182 has a large number of through holes 1 formed in the substrate 181.
In 83, by a calendar processing device (not shown),
A large number of through holes 183 press-fitted and formed in the substrate 181.
Corresponding to, a large number of absorptive regions 184 in a dot shape
It is regularly formed.

Although not shown exactly in FIG. 21, the approximately circular absorptive regions 184 having a size of about 0.01 mm 2 of about 10000 are regularly arranged at a density of about 5000 pieces / cm 2. It is formed on the substrate 181 of the biochemical analysis unit 180.

In this embodiment, the absorptive region 184 is formed.
The adsorptive film 182 is press-fitted into the through-hole 183 of the substrate 181 so that the surface of the substrate 181 and the surface of the substrate 181 are located at the same height to form the biochemical analysis unit 180.

As shown in FIG. 21, the magnetic recording layer 185 is embedded in the substrate 181, and the substrate 181 is also formed.
Include two circular through holes 186a, 18a for alignment.
6b is formed.

Also in this embodiment, various data are recorded in the magnetic recording layer 185 of the biochemical analysis unit 180, and the data recorded in the magnetic recording layer 185 is used in the same manner as in the above embodiment. The chemical analysis unit 180 and the stimulable phosphor sheet 90 are appropriately used and managed,
It becomes possible to carry out biochemical analysis with high accuracy.

FIG. 22 is a schematic perspective view of a biochemical analysis unit according to another preferred embodiment of the present invention.

As shown in FIG. 22, the biochemical analysis unit 190 according to this embodiment includes a substrate 191 made of aluminum, and the surface of the substrate 191 has a large number of absorptive regions made of nylon 6. 194 are regularly formed.

Although not accurately shown in FIG. 22,
The approximately circular adsorptive areas 194 having a size of about 0.01 square millimeters of about 10,000 are regularly formed on the surface of the aluminum substrate 191 at a density of about 5000 pieces / square centimeter.

As shown in FIG. 22, also in this embodiment, the magnetic recording layer 195 is embedded in the substrate 191, and two circular through holes 196a and 196b for positioning are formed in the substrate 191. Has been done.

Therefore, also in this embodiment, various data are recorded in the magnetic recording layer 195 of the biochemical analysis unit 190 as in the above embodiment, and the magnetic recording layer 1 is recorded.
By using the data recorded in 95, the biochemical analysis unit 190 and the stimulable phosphor sheet 90 can be appropriately used and managed, and the biochemical analysis can be executed with high accuracy.

FIG. 23 is a schematic partial sectional view of a biochemical analysis unit according to still another preferred embodiment of the present invention.

As shown in FIG. 23, the biochemical analysis unit 200 according to the present embodiment comprises an absorptive substrate 201 formed of nylon 6, and the absorptive substrate 20.
The porous plate 202 made of aluminum in which a large number of through holes 203 are formed is adhered to one surface of the porous plate 202.
A large number of absorptive regions 204 are formed by the absorptive substrate 201 in the through holes 203.

Although not shown exactly in FIG. 23,
The approximately circular through holes 203 having a size of about 0.01 square millimeters of about 10,000 are regularly formed on the perforated plate 202 made of aluminum with a density of about 5000 holes / square centimeter, and thus about 10,000 of the square holes. The substantially circular adsorptive regions 204 having a size of 0.01 mm 2 are regularly formed on the adsorptive substrate 201 at a density of about 5000 pieces / cm 2.

As shown in FIG. 23, a magnetic recording layer 205 is embedded in the surface of the porous plate 202.

Therefore, also in this embodiment, various data are recorded in the magnetic recording layer 205 of the biochemical analysis unit 200 in the same manner as in the above embodiment, and the magnetic recording layer 2 is recorded.
By using the data recorded in 05, the biochemical analysis unit 200 and the stimulable phosphor sheet 90 can be appropriately used and managed, and the biochemical analysis can be executed with high accuracy.

FIG. 24 is a schematic partial sectional view of a biochemical analysis unit according to still another preferred embodiment of the present invention.

As shown in FIG. 24, the biochemical analysis unit 210 according to this embodiment has a large number of recesses 213.
However, the substrate 211 made of aluminum formed regularly is provided, and the inner wall surface 213a of each recess 213 is covered with nylon 6 to form the absorptive region 214.

Although not shown exactly in FIG. 24, the substantially circular recesses 213 having a size of about 0.01 square millimeter of about 10,000 are regularly made of aluminum at a density of about 5000 pieces / square centimeter. Board 2
11 is formed.

As shown in FIG. 24, a magnetic recording layer 215 is embedded on the surface of the substrate 211.

Therefore, also in this embodiment, various data are recorded in the magnetic recording layer 215 of the biochemical analysis unit 210 as in the above-described embodiment, and the magnetic recording layer 2 is recorded.
By using the data recorded in 15, the biochemical analysis unit 210 and the stimulable phosphor sheet 90 can be appropriately used and managed, and the biochemical analysis can be executed with high accuracy.

The present invention is not limited to the above embodiments, and various modifications can be made within the scope of the invention described in the claims, and these are also included in the scope of the present invention. It goes without saying that it is a thing.

For example, in the above embodiment, the biochemical analysis units 1, 180, 190, 200, 210 are used.
In the magnetic recording layers 5, 185, 195 and 20 respectively.
5, 215 are provided, the magnetic recording layers 5, 18 are provided.
5, 195, 205, 215, an optical recording layer may be provided, and the data recording layer including a rewritable recording medium is a biochemical analysis unit 1, 180,
The type of recording medium is not particularly limited as long as it is formed in 190, 200, 210.

Further, in the above-mentioned embodiment, the data on the amount of the adsorptive material contained in the plurality of adsorptive regions 4, the kind of the specific binding substance dropped, and the position of the dropped adsorptive regions 4 are described. Data, data on the number of times the biochemical analysis unit 1 has been used, data on the date and time of hybridization, data on whether or not a radiolabeled substance has been used, biochemical analysis unit 1
When the biochemical analysis unit 1 and the specific stimulable phosphor sheet are provided to the user as one biochemical analysis kit,
Along with the biochemical analysis unit 1, one biochemical analysis kit is constituted, identification data for identifying the accumulative phosphor sheet to be used together, and adsorption on the absorptive region 4 of the biochemical analysis unit 1 are identified. Data that the user personally needs to manage the biochemical analysis unit 1, such as data that specifies the person who has collected the substance of biological origin that should be selectively hybridized to the specific binding substance , Biochemical analysis units 1, 180, 190, 2
00, 210 magnetic recording layers 5, 185, 195, 20
5 and 215, all of these data are stored in the biochemical analysis unit 1, 18
0, 190, 200, 210 magnetic recording layers 5, 185,
It is not always necessary to record it in 195, 205, 215, and some data may be stored in the biochemical analysis units 1, 1
80, 190, 200, 210 magnetic recording layers 5, 18
5, 195, 205, 215, on the other hand, on the other hand, in addition to all or part of these data, other data may be added to the biochemical analysis units 1, 18
0, 190, 200, 210 magnetic recording layers 5, 185,
You may make it record on 195, 205, 215.

Furthermore, in the above-mentioned embodiment, the specific binding substance adsorbed in the absorptive region 4 of the biochemical analysis unit 1 is
A biologically-derived substance labeled with a radioactive labeling substance contained in a hybridization solution and a biologically-derived substance labeled with a fluorescent substance are automatically and selectively hybridized to a hybridization device.
A read head 37 and a magnetic recording head 38 are provided to read the data recorded on the magnetic recording layer 5 of the biochemical analysis unit 1 and record the data on the magnetic recording layer 5 of the biochemical analysis unit 1. Using a hybridization device, the specific binding substance adsorbed in the absorptive region 4 of the biochemical analysis unit 1 is labeled with a radioactive labeling substance contained in the hybridization solution, and It is not always necessary to automatically and selectively hybridize a substance derived from a living body, which is labeled with a fluorescent substance, to a biochemical substance by using a separate data reading / recording device having a data reading and writing function. The data recorded on the magnetic recording layer 5 of the analysis unit 1 is read, and the magnetic recording of the biochemical analysis unit 1 is performed. 5, on which was recorded data,
Using a hybridization bag containing the hybridization solution or a hybridization container, the specific binding substance adsorbed in the adsorptive region 4 of the biochemical analysis unit 1 is labeled with a radioactive label contained in the hybridization solution. The substance of biological origin labeled with a substance and the substance of biological origin labeled with a fluorescent substance may be selectively hybridized.

Further, in the above embodiment, the exposure apparatus is provided with the read head 111 and the magnetic recording head 112, and the data recorded in the magnetic recording layer 5 of the biochemical analysis unit 1 by the read head 111 and the storability. The data recorded on the magnetic recording layer 94 of the phosphor sheet 90 is read, and the data is written on the magnetic recording layer 94 of the stimulable phosphor sheet 90 by the magnetic recording head 112. The magnetic recording layer 5 of the biochemical analysis unit 1 is not necessarily provided with the read head 111 and the magnetic recording head 112, and a separate data reading / recording device having a data reading and writing function is used. Recorded on the magnetic recording layer 94 of the stimulable phosphor sheet 90. Take seen, the magnetic recording layer 94 of the stimulable phosphor sheet 90 may be configured to write data.

Further, in the above-mentioned embodiment, the biochemical analysis unit 1 is used by using the electron microscope and the laser displacement meter.
The data on the amount of the adsorptive material contained in each of the adsorptive regions is generated. Is optional, and it is not always necessary to use an electron microscope and a laser displacement meter.

In the above embodiment, the biochemical analysis unit 1 used for N times and the stimulable phosphor sheet 90 used for M times are configured to be collected by the manufacturer for recycling. However, it is not always necessary to recycle the biochemical analysis unit 1 and the stimulable phosphor sheet 90, and it may be configured to be disposed of by the user. In that case, when a radiolabeled substance is used, the biochemical analysis unit 1 used is managed N times according to the date and time of hybridization recorded in the magnetic recording layer 5 of the biochemical analysis unit 1, The used stimulable phosphor sheet 90 is managed M times according to the date and time of exposure recorded on the magnetic recording layer 94 of the stimulable phosphor sheet 90, and is discarded at an appropriate time.

Furthermore, in the above-described embodiment, the exposure apparatus has the lid member locking mechanism including the hook member 105, the compression spring 106, the engaging groove 107 and the solenoid 108, but the exposure apparatus has a different mechanism. A lid member locking mechanism can also be provided.

Further, in the embodiment shown in FIGS. 1 to 20, the substrate 2 of the biochemical analysis unit 1 has 2 units.
Two positioning through holes 6a and 6b are formed, and two positioning through holes 96a and 96b are formed in the support 91 of the stimulable phosphor sheet 90.
Although it is formed at a position corresponding to b, it is not always necessary to form the positioning through holes 6a and 6b in the substrate 2 of the biochemical analysis unit 1, and the positioning through holes 96a and 96b are formed. It is not always necessary to form it on the support 91 of the stimulable phosphor sheet 90.

Further, in the above-mentioned embodiment, the substrate 2, 181, 191, 201, 211 of the biochemical analysis unit 1, 180, 190, 200, 210 has about 10 units.
Circular absorptive regions 4,184,194,204,21 having a size of about 0.01 square millimeters
4 are formed in a regular pattern with a density of about 5000 pieces / square centimeter, it is not always necessary to form the absorptive regions 4, 184, 194, 204, 214 in a substantially circular shape. It can be formed in any shape such as a rectangular shape.

Further, in the above-mentioned embodiment, the substrate 2, 181, 191, 201, 211 of the biochemical analysis unit 1, 180, 190, 200, 210 has about 100 units.
Generally circular absorptive regions 4, 184, 194, 204, 214 having a size of about 0.01 square millimeters of 00.
Are formed according to a regular pattern with a density of about 5000 pieces / square centimeter, but the number and size of the absorptive regions 4, 184, 194, 204, 214 are arbitrarily selected according to the purpose. You can
Preferably, the absorptive regions 4, 184, 194, 204, 2 having a size of 10 or more and less than 5 mm 2
14 are formed on the substrates 2, 181, 191, 201, 211 at a density of 10 pieces / square centimeter or more.

Furthermore, in the above-mentioned embodiment, the substrate 2, 181, 191, 201, 211 of the biochemical analysis unit 1, 180, 190, 200, 210 has about 10 units.
Circular absorptive regions 4,184,194,204,21 having a size of about 0.01 square millimeters
4 are formed according to a regular pattern with a density of about 5000 pieces / square centimeter, the absorptive regions 4, 184, 194, 204, 214 can be formed according to a regular pattern. Not necessarily required.

Further, in the above-mentioned embodiment, the support 91 of the stimulable phosphor sheet 90 has the same pattern as that of the absorptive region 4 of the biochemical analysis unit 1 and is about 1000.
A substantially circular stimulable phosphor layer region 92 having a size of 0 and a size of about 0.01 mm 2 is regularly formed at a density of about 5000 pieces / cm 2. It is not always necessary to form 92 into a substantially circular shape, and it may be formed into any shape such as a rectangular shape.

Further, in the above-mentioned embodiment, the support 91 of the stimulable phosphor sheet 90 has the same pattern as that of the absorptive region 4 of the biochemical analysis unit 1 and is about 1000.
A substantially circular stimulable phosphor layer region 92 having a size of 0 and a size of about 0.01 mm 2 is regularly formed at a density of about 5000 pieces / cm 2. The number and size of 92 can be arbitrarily selected according to the purpose, and preferably 10 or more and 10 or more stimulable phosphor layer regions 92 having a size of less than 5 mm 2 are used. Formed on the support 91 with a density of.

Further, in the above-mentioned embodiment, the support 91 of the stimulable phosphor sheet 90 has the same pattern as that of the absorptive region 4 of the biochemical analysis unit 1 and is about 1000.
A substantially circular stimulable phosphor layer region 92 having a size of 0 and a size of about 0.01 mm 2 is regularly formed at a density of about 5000 pieces / cm 2. It is not always necessary to form 92 on the support 91 of the stimulable phosphor sheet 90 in a regular pattern, and the stimulable phosphor layer region 92 is formed in the biochemical analysis units 1, 180, 190, It suffices that it is formed on the support 91 of the stimulable phosphor sheet 90 in the same pattern as the absorptive regions 4, 184, 194, 204, 214 of 200 and 210.

Furthermore, in the above embodiment, the stimulable phosphor layer region 92 of the stimulable phosphor sheet 90 is the absorptive region 4 formed in the biochemical analysis unit 1, 180, 190, 200, 210. , 184, 194, 204, 2
The photostimulable phosphor layer region 92 is formed to have the same size as that of the biochemical analysis unit 1, 180, 19
Adsorbent regions 4, 18 formed at 0, 200, 210
It is not always necessary to form the same size as 4, 194, 204, 214, and preferably the absorptive regions 4, 184, 194 formed in the biochemical analysis units 1, 180, 190, 200, 210, It is formed to have a size of 204 or 214 or more.

In the above embodiment, a plurality of c
Although DNA is used, the specific binding substance usable in the present invention is not limited to cDNA,
Hormones, tumor markers, enzymes, antibodies, antigens, abzymes, other proteins, nucleic acids, cDNA, DNA,
Any specific binding substance capable of specifically binding to a substance of biological origin, such as RNA, and having a known base sequence, base length, composition, etc. can be used as the specific binding substance of the present invention. .

Further, in the above embodiment, the absorptive regions 4, 184, 194, 204, 214 of the biochemical analysis units 1, 180, 190, 200, 210 are all formed of nylon 6. , The absorptive area 4, 184, 194, 204, 214 is nylon 6
It is not always necessary that the porous material is formed of a porous material other than nylon 6, such as nylon 6,6 and nylon 4,10; nitrocellulose, cellulose acetate,
Cellulose derivatives such as cellulose acetate butyrate; collagen; alginic acid, calcium alginate, alginic acid /
Alginic acids such as polylysine polyion complex; polyolefins such as polyethylene and polypropylene; polyvinyl chloride; polyvinylidene chloride; polyfluoride such as polyvinylidene fluoride and polytetrafluoride; and copolymers or composites thereof, or The absorptive regions 4, 184, 194, 204, 214 of the biochemical analysis units 1, 180, 190, 200, 210 can be formed by a porous carbon material such as activated carbon, and further, platinum, gold, Iron, silver, nickel,
Metals such as aluminum; metal oxides such as alumina, silica, titania, zeolite; metal salts such as hydroxyapatite and calcium sulfate; inorganic porous materials such as composites of these; or bundles of multiple fibers for biochemical analysis Units 1, 180, 190, 200, 21
It is also possible to form zero absorptive regions 4, 184, 194, 204, 214.

In the above embodiment, the biochemical analysis units 1, 180, 190, 200 and 210 are all made of aluminum substrates 2, 181, 191, and 2.
01, 211, but the substrates 2, 18 of the biochemical analysis units 1, 180, 190, 200, 210
It is not always necessary to form 1, 191, 201, 211 by aluminum, and the substrate 2, 181, 191, 201, 211 can be formed by other materials. Biochemical analysis units 1, 180, 1
90, 200, 210 substrates 2, 181, 191, 20
It is preferable that Nos. 1 and 211 have a property of attenuating radiation and preferably have a property of attenuating light, but the material thereof is not particularly limited, and it is an inorganic compound material. , Organic compound materials, biochemical analysis units 1, 180, 190, 20
0, 210 substrates 2, 181, 191, 201, 211
May be formed, and metallic materials, ceramic materials or plastic materials are particularly preferably used. Examples of the inorganic compound material that can be preferably used for forming the substrates 2, 181, 191, 201, 211 of the biochemical analysis units 1, 180, 190, 200, 210 include gold, silver, copper, and the like. Zinc, aluminum, titanium, tantalum, chromium, iron, nickel, cobalt,
Metals such as lead, tin and selenium; alloys such as brass, stainless steel and bronze; silicon, amorphous silicon, glass,
Silicon materials such as quartz, silicon carbide and silicon nitride; metal oxides such as aluminum oxide, magnesium oxide and zirconium oxide; inorganic salts such as tungsten carbide, calcium carbonate, calcium sulfate, hydroxyapatite and gallium arsenide. . These may have any structure in a polycrystalline sintered body such as single crystal, amorphous, or ceramic. A polymer compound is preferably used as the organic compound material that can be preferably used for forming the substrates 2, 181, 191, 201, 211 of the biochemical analysis units 1, 180, 190, 200, 210. Examples of preferable polymer compounds include polyolefins such as polyethylene and polypropylene; acrylic resins such as polymethyl methacrylate and butyl acrylate / methyl methacrylate copolymers; polyacrylonitrile; polyvinyl chloride; polyvinylidene chloride; polyvinylidene fluoride; Polytetrafluoroethylene; Polychlorotrifluoroethylene; Polycarbonate; Polyester such as polyethylene naphthalate and polyethylene terephthalate; Nylon 6, nylon 6,6, nylon 4,10, etc. Ron; Polyimide; Polysulfone; Polyphenylene sulfide; Silicon resins such as polydiphenylsiloxane; Phenolic resins such as novolak; Epoxy resins; Polyurethane; Polystyrene; Butadiene-styrene copolymers; Cellulose, cellulose acetate, nitrocellulose, starch, calcium alginate, Examples thereof include polysaccharides such as hydroxypropylmethyl cellulose; chitin; chitosan; sumacum; polyamides such as gelatin, collagen and keratin, and copolymers of these polymer compounds. These may be composite materials, and if necessary, metal oxide particles, glass fibers and the like can be filled, and by blending an organic compound material,
It can also be used.

Also, in the embodiment shown in FIG. 21, the absorptive region 184 of the biochemical analysis unit 180 is used.
Is formed by press-fitting the adsorptive film 182 into a large number of through holes 183 formed in the aluminum substrate 181 using a calendering device. However, other means such as a hot press device may be used. The adsorptive film 182 is used to form the substrate 18
It is also possible to press-fit into the through-hole 183 of No. 1, and instead of press-fitting, the adsorbing film 182 is embedded in the through-hole 183 of the substrate 181 by an appropriate method to form the dot-shaped adsorbing region 184. You may do it.

Further, in the above-mentioned embodiment, the biochemical analysis units 1, 180, 190, 200 and 210 are all absorptive regions 4, 184 and 19 which are separated from each other.
4, 204, 214, the biochemical analysis unit does not necessarily need to have the absorptive regions separated from each other.

Further, in the embodiment shown in FIGS. 1 to 20, a large number of absorptive regions 4 of the biochemical analysis unit 1 are absorptive in a large number of through holes 3 formed in the substrate 2. Formed by filling the material,
Instead of the through holes 3, a large number of recesses may be formed in the substrate 2, and the large number of recesses may be filled with an adsorptive material to form an adsorptive region.

Furthermore, in the embodiment shown in FIG. 22, the absorptive region 194 of the biochemical analysis unit 190 is used.
Is formed on the surface of the substrate 191, a large number of protrusions are regularly formed on the surface of the substrate 191 of the biochemical analysis unit 190, and an absorptive region is formed at the tip of each protrusion. It may be formed.

In the above embodiment, the stimulable phosphor sheet 90 is provided with a nickel support 91 having a large number of substantially circular through holes 93 formed therein. The stimulable phosphor is filled in the hole 93 to form the stimulable phosphor layer region 92. Instead of the through hole 93, a large number of substantially circular recesses are regularly formed in the support 91. The stimulable phosphor layer region 92 on the dots can also be formed by embedding the photostimulable phosphor in the recesses and burying it in the recesses.

Further, in the above-mentioned embodiment, the stimulable phosphor sheet 90 has the support 91 made of nickel, but it is not always necessary to form the support 91 of the stimulable phosphor sheet 90 by nickel. Alternatively, the support body 91 can be formed of other materials. The support 91 of the stimulable phosphor sheet 90 preferably has a property of attenuating radiation and preferably a property of attenuating light, but the material thereof is not particularly limited. The support 91 of the stimulable phosphor sheet 90 may be formed of either an inorganic compound material or an organic compound material, and a metal material, a ceramic material, or a plastic material is particularly preferably used. Examples of the inorganic compound material that can be preferably used to form the support 91 of the stimulable phosphor sheet 90 include, for example, gold, silver, copper, zinc, aluminum, titanium, tantalum, chromium, iron, nickel, cobalt. ,
Metals such as lead, tin and selenium; alloys such as brass, stainless steel and bronze; silicon, amorphous silicon, glass,
Silicon materials such as quartz, silicon carbide and silicon nitride; metal oxides such as aluminum oxide, magnesium oxide and zirconium oxide; inorganic salts such as tungsten carbide, calcium carbonate, calcium sulfate, hydroxyapatite and gallium arsenide. . These may have any structure in a polycrystalline sintered body such as single crystal, amorphous, or ceramic. Further, a polymer compound is preferably used as an organic compound material that can be preferably used for forming the support 91 of the stimulable phosphor sheet 90, and a preferable polymer compound is, for example, polyethylene or polypropylene. Polyolefins; Acrylic resins such as polymethylmethacrylate and butylacrylate / methylmethacrylate copolymers; Polyacrylonitrile; Polyvinyl chloride; Polyvinylidene chloride; Polyvinylidene fluoride; Polytetrafluoroethylene; Polychlorotrifluoroethylene; Polycarbonate; Polyethylene Polyester such as phthalate and polyethylene terephthalate; nylon 6,
Nylon such as nylon 6,6 and nylon 4,10; polyimide; polysulfone; polyphenylene sulfide; silicon resin such as polydiphenylsiloxane; phenol resin such as novolac; epoxy resin; polyurethane; polystyrene; butadiene-styrene copolymer;
Polysaccharides such as cellulose, cellulose acetate, nitrocellulose, starch, calcium alginate, hydroxypropylmethylcellulose; chitin; chitosan; sumac;
Examples thereof include polyamides such as gelatin, collagen and keratin, and copolymers of these high molecular compounds. These may be composite materials, if necessary, it is possible to fill with metal oxide particles or glass fibers,
Also, an organic compound material can be blended and used.

In the above-mentioned embodiment, a hybridization solution containing a biological substance labeled with a radioactive labeling substance and a biological substance labeled with a fluorescent substance such as a fluorescent dye is prepared, and the absorptive region 4 Although it is hybridized with the specific binding substance dropped on the, the substance derived from the living body does not necessarily need to be labeled with a fluorescent substance such as a radiolabeling substance and a fluorescent dye, and a radiolabeling substance, a fluorescent substance And a labeling substance selected from the group consisting of labeling substances that generate chemiluminescence when brought into contact with a chemiluminescent substrate.

Further, in the above-mentioned embodiment, the substance of biological origin labeled with a radioactive labeling substance and a fluorescent substance such as a fluorescent dye is hybridized with the specific binding substance. It is not always necessary to hybridize with a specific binding substance,
Instead of using a substance derived from a living body for hybridization,
It is also possible to specifically bind to a specific binding substance by an antigen-antibody reaction or a reaction such as a receptor / ligand.

Further, in the above-mentioned embodiment, the exposure apparatus is constructed so that the stimulable phosphor sheet 90 is superposed on the biochemical analysis unit 1 set on the substrate 102 and exposed. But the stimulable phosphor sheet 9
It is also possible to configure the exposure apparatus so that 0 is set on the substrate 102, the biochemical analysis unit 1 is superposed on it, and exposure is performed.

Furthermore, in the above embodiment, FIG.
Through the scanner shown in FIG. 20 to FIG. 20, a unit for radiation data and biochemical analysis of radiolabeled substances recorded in a large number of dot-shaped stimulable phosphor layer regions 92 formed on the stimulable phosphor sheet 90. Although the fluorescence data of the fluorescent substance recorded in the absorptive region 4 of No. 1 is read to generate the data for biochemical analysis, the radiation data of the radiolabeled substance and the fluorescence data of the fluorescent substance are read by one scanner. The radiation data of the radiolabeled substance and the fluorescence data of the fluorescent substance may be read by separate scanners to generate the biochemical analysis data.

In the above embodiment, the control unit 170 controls the first laser pumping light source 121 and the second laser pumping light source 122 to be turned on / off in synchronization with the intermittent movement of the optical head 135. However, in the main scanning direction, the moving speed of the optical head 135 in the main scanning direction is determined so that the laser light 24 moves quickly between the adjacent photostimulable phosphor layer regions 92 or the adsorbing regions 4. Then, the first laser excitation light source 121
And holding the second laser excitation light source 122 in the ON state, the optical head 135 is simply moved intermittently,
Many stimulable phosphor layer regions 92 or absorptive regions 4
Are sequentially scanned with a laser beam 124, and photostimulated light 145 emitted from the stimulable phosphor layer region 92 or fluorescence 145 emitted from the absorptive region 4 is photoelectrically detected to perform biochemical analysis. It is also possible to generate data for use.

The scanner shown in FIGS. 13 to 20 is provided with a first laser pumping light source 121, a second laser pumping light source 122 and a third laser pumping light source 123, but three laser pumping light sources are used. It is not always necessary to have a light source.

Further, in the scanner shown in FIGS. 13 to 20, the scanner is the laser light 1 having a wavelength of 640 nm.
First laser excitation light source 121 emitting 24 and 532n
The second laser excitation light source 122 that emits the laser light 124 having a wavelength of m and the third laser excitation light source 123 that emits the laser light 124 having a wavelength of 473 nm are provided, but the laser excitation light source is used as the excitation light source. It is not always necessary to use, instead of the laser excitation light source, an LED light source can be used as an excitation light source, and further, a halogen lamp is used as an excitation light source, and a spectral filter cuts wavelength components that do not contribute to excitation. You may do it.

In the above embodiment, the optical head 1 is moved by the scanning mechanism in the main scanning direction indicated by arrow X and the sub-scanning direction indicated by arrow Y in FIG.
By moving 35, all the dot-shaped stimulable phosphor layer areas 92 of the stimulable phosphor sheet 90 or all the dot-shaped absorptive areas 4 of the biochemical analysis unit 1 are scanned by the laser light 124. Then, the photostimulable phosphor or the fluorescent substance is excited.
5 is kept stationary and the stage 140 is moved in the main scanning direction indicated by arrow X and the sub scanning direction indicated by arrow Y in FIG.
24 scans all the dot-shaped stimulable phosphor layer areas 92 of the stimulable phosphor sheet 90 or all the dot-shaped absorptive areas 4 of the biochemical analysis unit 1 to scan the stimulable phosphor or The fluorescent substance may be excited, and the optical head 135 may be moved in the main scanning direction indicated by arrow X or the sub-scanning direction indicated by arrow Y in FIG. It can also be moved in the sub-scanning direction indicated by or the main scanning direction indicated by arrow X.

Further, in the scanner shown in FIG. 13 to FIG. 20, the photomultiplier 150 is used as the photodetector, and the fluorescence 145 or the stimulated emission 145 is used.
Is detected photoelectrically, but as a photodetector, fluorescence 1
It is only necessary to photoelectrically detect 45 or stimulated emission 145, and not only the photomultiplier 150 but also other photodetectors such as a photodiode or a line CCD or a two-dimensional CCD can be used.

Further, in the above embodiment, the spotting head 9 of the spotting device is moved by the drive mechanism in the main scanning direction indicated by arrow X and the sub scanning direction indicated by arrow Y in FIG. Although the chemically binding substance is dropped onto the absorptive region 4 formed on the substrate 2 of the biochemical analysis unit 1, the spotting head 9 of the spotting device is maintained in a stationary state, and the biochemical analysis unit 1 is In FIG. 3, the specific binding substance is moved to the absorptive region 4 formed on the substrate 2 of the biochemical analysis unit 1 by moving in the main scanning direction indicated by an arrow X and the sub scanning direction indicated by an arrow Y. Alternatively, the spotting head 9 of the spotting device may be moved to the main scanning direction indicated by arrow X in FIG. While moving in the sub-scanning direction shown, the biochemical analysis unit 1 is moved in the sub-scanning direction shown by the arrow Y or the main scanning direction shown by the arrow X in FIG. It may be dropped onto the absorptive region 4 formed on the substrate 2 of the biochemical analysis unit 1.

Furthermore, in the above-mentioned embodiment, the specific binding substance is dropped on the absorptive region 4 formed on the substrate 2 of the biochemical analysis unit 1 by a spotting device having a drive mechanism. Injector 6 and CCD
Using a spotting device equipped with a camera 7, a CCD camera 7 is used to detect the tip of the injector 6 and the cDNA.
While observing the absorptive region 4 to which the specific binding substance such as is dropped, when the tip of the injector 6 and the center of the absorptive region 4 to which the specific binding substance such as cDNA is dropped, A specific binding substance such as cDNA may be released from the injector 6.

[0516]

According to the present invention, the data unique to each biochemical analysis unit such as a microarray or a macroarray can be reliably managed, and the reliability of biochemical analysis can be significantly improved. It becomes possible to provide a biochemical analysis unit that can be used.

[Brief description of drawings]

FIG. 1 is a schematic perspective view of a biochemical analysis unit according to a preferred embodiment of the present invention.

FIG. 2 is a schematic front view of a spotting device.

FIG. 3 is a schematic plan view of a spotting device.

FIG. 4 is a block diagram showing a control system, an input system, a drive system, a detection system and a recording system of the spotting device.

FIG. 5 is a schematic side view of a hybridization device.

FIG. 6 is a schematic perspective view of a cartridge.

FIG. 7 is a block diagram of a control system, a detection system, a drive system, an input system, and a display system of the hybridization device.

FIG. 8 is a schematic perspective view of a stimulable phosphor sheet.

FIG. 9 is a view showing that a large number of stimulable phosphor layer regions formed on a stimulable phosphor sheet are selectively included in a large number of absorptive regions formed in a biochemical analysis unit. It is a schematic perspective view of the exposure apparatus which exposes with a radioactive labeling substance.

FIG. 10 is a schematic partial cross-sectional view showing a mechanism for locking the lid member of the stimulable phosphor sheet exposure apparatus to the casing.

FIG. 11 is a block diagram showing a control system, a detection system, a drive system and a display system of the exposure apparatus for the stimulable phosphor sheet.

FIG. 12 shows a large number of dot-shaped stimulable phosphors formed on a stimulable phosphor sheet by a radiolabel substance contained in a large number of absorptive regions formed in a biochemical analysis unit. FIG. 7 is a schematic cross-sectional view showing a method of exposing a layer region.

FIG. 13 is a diagram showing the radiation data recorded on the stimulable phosphor sheet to generate biochemical analysis data, and the fluorescence data recorded on the biochemical analysis unit to read the biochemical analysis data. 3 is a schematic perspective view of a scanner that generates analysis data. FIG.

14 is a schematic perspective view showing details of the vicinity of the photomultiplier of the scanner shown in FIG.

15 is a schematic cross-sectional view taken along the line AA of FIG.

16 is a schematic cross-sectional view taken along the line BB of FIG.

FIG. 17 is a schematic cross-sectional view taken along the line CC of FIG.

FIG. 18 is a schematic cross-sectional view taken along the line DD of FIG.

FIG. 19 is a schematic plan view of a scanning mechanism of an optical head.

FIG. 20 is a block diagram showing a control system, an input system, a drive system and a detection system of the scanner.

FIG. 21 is a schematic perspective view of a biochemical analysis unit according to another preferred embodiment of the present invention.

FIG. 22 is a schematic perspective view of a biochemical analysis unit according to another preferred embodiment of the present invention.

FIG. 23 is a schematic partial cross-sectional view of a biochemical analysis unit according to still another preferred embodiment of the present invention.

FIG. 24 is a schematic partial cross-sectional view of a biochemical analysis unit according to still another preferred embodiment of the present invention.

[Explanation of symbols]

1 Biochemical Analysis Unit 2 Substrate 3 Through Hole 4 Adsorption Area 5 Magnetic Recording Layers 6a, 6b Positioning Through Hole 7 Injector 8 CCD Camera 9 Spotting Head 10 Substrate 11 Frame 12 Sub-scanning Pulse Motor 13 Rail 14 Movable Substrate 15 Rod 16 Main scanning pulse motor 17 Endless belt 18 Linear encoder 19 Linear encoder slits 20a, 20b Positioning pin 25 Control unit 26 Keyboard 27 Magnetic recording head 30 Hybridization device 31 Cartridge 31a Cartridge casing 31b Cartridge lid 31c Solution injection Extraction port 32 Cartridge loading section 33 Solution injection section 34 Reaction section 35 Biochemical analysis unit removal section 36a First endless belts 36b, 36c Pulley 37 read head 38 magnetic recording head 39 loading mechanism 40a second endless belts 40b and 40c pulley 41a third endless belts 41b and 41c pulley 42 pretreatment liquid injection pin 43 hybridization solution injection pin 44 probe solution injection pin 45 cleaning solution Injection pin 46 Solution pin head 47a Fourth endless belt 47b, 47c Pulley 48 Vibration table 49a Fifth endless belt 49b, 49c Pulley 50 RI sensor 51 Solution extraction pin 52 Biochemical analysis unit removal mechanism 60 Control unit 61 First Motor 62 Second motor 63 Third motor 64 Fourth motor 65 Fifth motor 66 Vibration table motor 67 Injection pin motor 68 RI sensor motor 69 Solution extraction pin motor 70 Pretreatment Liquid pump 71 Hybridization solution pump 72 Probe solution pump 73 Washing solution pump 74 Solution extraction pump 75 Valve opening / closing mechanism 80 Keyboard 81 Display panel 90 Accumulative phosphor sheet 91 Support 92 Photostimulable phosphor layer region 93 Through hole 94 Magnetic Recording layer 96a, 96b Positioning through hole 100 Casing 101 Cover member 102 Substrate 103 Data reading / recording unit 104a, 104b Positioning pin 105 Hook member 105a Hook member shaft 106 Compression spring 107 Engaging groove 108 Solenoid 110 Control unit 111 read head 112 magnetic recording head 113 display panel 114 cover member release button 121 first laser excitation light source 122 second laser excitation light source 123 third laser excitation light source 124 laser light 25 Collimator Lens 126 Mirror 127 First Dichroic Mirror 128 Second Dichroic Mirror 129 Mirror 130 Collimator Lens 131 Collimator Lens 132 Mirror 133 Perforated Mirror Hole 134 Perforated Mirror 135 Optical Head 136 Mirror 137 Aspherical Lens 138 Concave Mirror 140 Stage 141 Glass plate 145 Fluorescent or stimulable light 148 Filter unit 150 Photomultipliers 151a, 151b, 151c, 151d Filter members 152a, 152b, 152c, 152d Filter 153 A / D converter 154 Data processing device 160 Substrate 161 Sub-scanning pulse Motor 162 Pair of rails 163 Movable substrate 164 Rod 165 Main scanning stepping motor 166 Endless belt 167 Encoder 168 Linear encoder slit 170 Control unit 171 Keyboard 172 Filter unit Motor 173 Reader 180 Biochemical analysis unit 181 Substrate 182 Adsorbent film 183 Through hole 184 Adsorbable region 185 Magnetic recording layer 186a, 186b Positioning through hole 190 Biochemical analysis unit 191 Substrate 194 Adsorption region 195 Magnetic recording layers 196a, 196b Positioning through-hole 200 Biochemical analysis unit 201 Adsorption substrate 202 Perforated plate 203 Through-hole 204 Adsorption region 205 Magnetic recording layer 210 Biochemistry Analysis unit 211 Substrate 213 Recess 213a Recess inner wall surface 214 Adsorbable region 215 Magnetic recording layer

Claims (26)

[Claims] 1. A biochemical analysis unit comprising a substrate, and a data recording layer including a rewritable recording medium is formed on the substrate. 2. A plurality of spot-shaped regions are formed by dropping a specific binding substance capable of specifically binding to a substance of biological origin and having a known base sequence, base length, composition, etc. on the substrate. The biochemical analysis unit according to claim 1, wherein the biochemical analysis unit is formed. 3. The data recording layer comprises a first data recording area in which the user cannot rewrite data and a second data recording area in which the user can rewrite data.
The biochemical analysis unit according to claim 1 or 2, further comprising a data recording area. 4. The first data recording area of the data recording layer is recorded with data relating to the type of specific binding substance and the position of a spot-shaped area containing each specific binding substance. The biochemical analysis unit according to claim 2 or 3. 5. The number of times of use of the biochemical analysis unit can be recorded in the first data recording area of the data recording layer according to any one of claims 1 to 4. Biochemical analysis unit described. 6. The use date of the biochemical analysis unit is recordable in the first data recording area and / or the second data recording area of the data recording layer. Item 6. The biochemical analysis unit according to any one of items 1 to 5. 7. The first data recording area of the data recording layer is configured to be able to record the number of recycles of the biochemical analysis unit in the first data recording area.
The unit for biochemical analysis according to any one of 1. 8. The biochemical analysis according to any one of claims 1 to 7, wherein a plurality of absorptive regions are two-dimensionally formed on the substrate so as to be separated from each other. unit. 9. A plurality of holes are two-dimensionally formed in the substrate so as to be spaced apart from each other, and the plurality of absorptive regions are formed by filling the holes with an absorptive material, respectively. The biochemical analysis unit according to claim 8, which is characterized in that. 10. A plurality of through-holes are formed in the substrate in a two-dimensional manner so as to be separated from each other, and the plurality of absorptive regions are formed of an absorptive film containing an absorptive material. The biochemical analysis unit according to claim 9, wherein the biochemical analysis unit is formed by being press-fitted into the through hole of the. 11. An adsorbent substrate comprising an adsorbent material,
A plurality of through holes are formed in the substrate in a two-dimensional manner so as to be spaced apart from each other, and the substrate is adhered to at least one surface of the absorptive substrate to form the plurality of through holes formed in the substrate. The biochemical analysis unit according to claim 8, wherein the plurality of absorptive regions are formed by the absorptive substrate in the holes. 12. A layer of an absorptive material in which a plurality of recesses are formed on the substrate so as to be two-dimensionally spaced apart from each other, and the plurality of absorptive regions are respectively formed on the inner wall surface of the recess. The biochemical analysis unit according to claim 8, which is formed by: 13. The biochemical analysis unit according to claim 8, wherein the plurality of absorptive regions are formed on the surface of the substrate. 14. A plurality of protrusions are formed on the substrate so as to be two-dimensionally spaced apart from each other, and the plurality of absorptive regions are respectively formed in the vicinity of the tips of the protrusions. The biochemical analysis unit according to claim 8. 15. The biochemical analysis unit according to claim 8, wherein 10 or more absorptive regions are formed on the substrate. 16. The plurality of absorptive regions are respectively
The biochemical analysis unit according to any one of claims 8 to 15, having a size of less than 5 mm 2. 17. The biochemical analysis according to any one of claims 8 to 16, wherein the plurality of absorptive regions are formed on the substrate at a density of 10 or more per cm 2. unit. 18. The biochemical analysis unit according to claim 8, wherein the absorptive region is regularly formed. 19. The biochemical analysis unit according to any one of claims 8 to 12 and 15 to 18, wherein the substrate has a property of attenuating radiation. 20. The substrate has a property of attenuating the energy of the radiation to ⅕ or less when the radiation penetrates through the substrate by a distance equal to the distance between the adsorbing regions adjacent to each other. The biochemical analysis unit according to claim 19, which is characterized in that. 21. The biochemical analysis unit according to any one of claims 8 to 12 and 15 to 20, wherein the substrate has a property of attenuating light. 22. The substrate has a property of attenuating the energy of light to ⅕ or less when light is transmitted through the substrate by a distance equal to the distance between the adsorbing regions adjacent to each other. 22. The biochemical analysis unit according to claim 21, wherein: 23. The biochemical analysis according to claim 19, wherein the substrate is made of a material selected from the group consisting of a metal material, a ceramic material and a plastic material. unit. 24. The data relating to the plurality of absorptive areas is recordable in the first data recording area of the data recording layer.
The biochemical analysis unit according to any one of 3 above. 25. The biochemical analysis unit according to claim 1, wherein the substrate is an absorptive substrate formed of an absorptive material. 26. The biochemical analysis unit according to claim 1, wherein the substrate is formed of a slide glass plate.