JP2002003393A - Fibroblast growth agent, food and drink for beauty culture and skin cosmetic - Google Patents

Abstract

PROBLEM TO BE SOLVED: To find out a substance effective in promoting the growth of fibroblasts and a substance having skin beautifying actions or aging preventing actions on the skin and to obtain a fibroblast growth agent, a food and drink for beauty culture and a skin cosmetic comprising either one of the substances as an active ingredient. SOLUTION: The fibroblast growth agent, skin cosmetic or food and drink for beauty culture is obtained by including an extract from pigment-containing rice or a rice bran of the pigment-containing rice.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

[0001] The present invention relates to a fibroblast-proliferating agent, a cosmetic food and drink, and a skin cosmetic.

[0002]

2. Description of the Related Art The epidermis and dermis of skin are composed of epidermal cells, fibroblasts, and extracellular matrices such as collagen outside these cells and supporting the skin structure.

[0003] In young skin, fibroblasts are actively proliferating, and the interaction of skin tissues such as fibroblasts and collagen secures water retention, flexibility, elasticity, and the like. It is maintained in a glossy and fresh state.

However, there are certain external factors such as ultraviolet rays, remarkable drying of air, and excessive skin washing,
As aging progresses, the proliferation of fibroblasts slows down and the moisturizing function and elasticity of the skin and the like decrease. Then, the skin loses its tone and luster, and exhibits aging symptoms such as roughness and wrinkles.

[0005] The most common practice for preventing and improving skin aging by the above mechanism is protection of the skin surface. For example, by applying an external preparation having a moisturizing effect such as a lotion, a cream, and a milky lotion to the skin,
Evaporation of water from the skin is prevented, the water content of the skin is maintained, and the skin is moisturized.

However, such an external preparation only improves the keratin condition of the epidermis, and cannot be expected to improve the tension holding mechanism in the dermis. Also, since moisturizers slow the evaporation of water from the skin,
In general, the feeling of use is poor, and even long-term use may even cause skin damage.

[0007]

SUMMARY OF THE INVENTION Therefore, the present invention firstly finds a substance having an action of promoting the proliferation of fibroblasts that synthesize collagen, and uses the substance as an active ingredient to act as a fibroblast proliferation agent. The purpose is to provide. Another object of the present invention is to provide a cosmetic food / beverage product and a skin cosmetic capable of exhibiting a beautifying effect or preventing skin aging by promoting the proliferation of fibroblasts that synthesize collagen. I do.

[0008]

In order to achieve the above-mentioned object, the present invention firstly provides a fiber comprising a pigmented rice or an extract from pigmented rice bran as an active ingredient. Secondly, the present invention provides a blast proliferation agent and, secondly, a cosmetic food / beverage product and skin cosmetic comprising pigmented rice or an extract from pigmented rice bran. In a preferred embodiment of the agent for acting on fibroblast proliferation of the present invention, the extract is an extract obtained using a polar solvent as an extraction solvent. In a preferred embodiment of the fibroblast proliferation-promoting agent of the present invention, the polar solvent is water, methanol, ethanol, propanol, 1,3-butylene glycol, glycerin, propylene glycol or a mixture of two or more thereof. is there.

[0009]

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in detail.

[Fibroblast Proliferating Agent] The fibroblast growing agent of the present invention is characterized by containing pigmented rice or an extract from pigmented rice bran as an active ingredient.

[0011] In the agent for promoting fibroblast proliferation of the present invention,
The “extract” includes an extract obtained from an extraction raw material by an extraction treatment, a diluent or a concentrate of the extract, a dried product obtained by drying the extract, or a crude product or a purified product thereof. Both are included.

[0012] In the agent for promoting fibroblast proliferation of the present invention,
What is used as an extraction raw material in the extraction treatment is pigmented rice or pigmented rice bran.

[0013] Here, "pigmented rice" refers to a pigment (eg, an anthocyanin-based purple pigment, a tannin-based red pigment) in a bran layer (a portion that becomes a bran and peels off during milling).
Means rice with Also, "pigmented rice bran"
It means a powdery mixture of pericarp, seed coat, ectosperm, etc., which is produced when milling pigmented rice.

In the agent for promoting fibroblast proliferation of the present invention,
Pigmented rice used as an extraction raw material may be in any processing stage as long as it has a bran layer, for example, a state with rice husks (rice rice), a state with rice husks removed (brown rice),
Pigmented rice in any state, such as a state in which a part of rice husk is removed or a state in which a part of a bran layer is removed, may be used as an extraction raw material. Is preferably used as an extraction raw material.

As described above, the bran layer of the pigmented rice contains an anthocyanin-based purple pigment, a tannin-based red pigment, and the like. ) Can be easily distinguished from general brown rice, which is pale red to purple black and brown, by its appearance. Also, the bran produced when milling pigmented rice can be easily distinguished from general white rice bran by its appearance.

There are various types of pigmented rice such as purple rice, black rice, red rice, red rice and the like, and there are various varieties and localities. In the fibroblast-proliferating agent of the present invention, any one of them is used. Pigmented rice of its type, variety, locality, etc. or its bran may be used as an extraction raw material.

In the agent for promoting fibroblast proliferation of the present invention,
Specific examples of pigmented rice that can be preferably used as an extraction raw material include purple rice, such as southern purple from Guangdong, China, and morning purple from Akita.For black rice, black dominance from Nagano, etc. For red rice, red rice from Kyushu can be exemplified, and for red rice, red rice from Yunnan, China can be exemplified.

As the pigmented rice or pigmented rice bran, one kind may be used alone as an extraction raw material, or two or more kinds may be used as an extraction raw material in combination.

Pigmented rice or pigmented rice bran which has been subjected to a pretreatment such as defatting with a nonpolar solvent such as hexane or benzene may be used as an extraction raw material. By performing a pretreatment such as degreasing, extraction of pigmented rice or bran of pigmented rice with a polar solvent can be performed efficiently.

In the fibroblast-proliferating agent of the present invention,
In the case of the extraction treatment, it is preferable to use a polar solvent as the extraction solvent. Although the component showing the fibroblast proliferation activity contained in pigmented rice or pigmented rice bran has not yet been identified, its component is extracted from pigmented rice or pigmented rice by an extraction treatment using a polar solvent as an extraction solvent. It can be easily extracted from bran.

Specific examples of suitable polar solvents include water, lower aliphatic alcohols, and hydrated lower aliphatic alcohols. These may be used alone or in combination of two or more. be able to. Specific examples of suitable lower aliphatic alcohols include methanol, ethanol, propanol, 1,3-butylene glycol, glycerin, propylene glycol and the like.

When a mixture of two or more polar solvents is used as the extraction solvent, the mixing ratio can be appropriately adjusted. For example, when a mixed solution of water and a lower aliphatic alcohol is used, the mixing ratio of the water and the lower aliphatic alcohol can be 2: 1 to 1: 6 (weight ratio).

As the extraction solvent, it is particularly preferable to use water among the above polar solvents. Water that can be used as the extraction solvent includes pure water, tap water, well water, mineral water, mineral water, hot spring water, spring water, fresh water, and the like, as well as those subjected to various treatments. Examples of the treatment applied to water include heating,
This includes sterilization, sterilization, filtration, ion exchange, adjustment of osmotic pressure, buffering, and the like. Therefore, water that can be used as the extraction solvent includes hot water, ion-exchanged water, physiological saline, phosphate buffer,
Phosphate buffered saline and the like are also included.

The extraction treatment is not particularly limited as long as the soluble components contained in the pigmented rice or the bran of the pigmented rice can be eluted into the extraction solvent, and can be carried out according to a conventional method. In the extraction treatment, it is not necessary to employ a special extraction method, and any device can be used at room temperature or under reflux.

For example, a raw material for extraction is put into a treatment tank filled with an extraction solvent, and a soluble component can be eluted with occasional stirring. At this time, the amount of the extraction solvent is not particularly limited, and can be appropriately adjusted according to the amount of the extraction raw material.
It is usually 500 to 2000 ml for 100 g. The extraction time is usually 1 to 6 hours, and the extraction temperature is usually 40 to 90 ° C.

After the soluble component is eluted by the extraction treatment, the extract is obtained by filtering to remove the extraction residue. The obtained extract is diluted according to a conventional method to obtain a diluent or concentrate of the extract, a dried product of the extract, or a crude product or a purified product thereof.
Treatment such as concentration, drying, and purification may be performed. In obtaining a dried extract, a carrier such as dextrin or cyclodextrin may be added to improve the hygroscopicity.

[0027] Pigmented rice or an extract from pigmented rice bran has a unique odor and taste, and may be decolorized or deodorized to the extent that its physiological activity is not reduced.

The pigmented rice or the extract from pigmented rice bran obtained as described above has a fibroblast proliferation effect. Although the mechanism of action of the fibroblast proliferation action of the pigmented rice or its extract from bran has not been elucidated so far, it is described in the Examples below that this extract has the fibroblast proliferation action. The agent for fibroblast proliferation of the present invention utilizes the fibroblast proliferation action of pigmented rice or an extract from pigmented rice bran.

Pigmented rice or an extract from pigmented rice bran can be used as it is as a fibroblast-proliferating agent, but can also be formulated into a conventional method and used. In the case of formulation, a pharmaceutically acceptable carrier such as dextrin and cyclodextrin and other optional auxiliaries can be added to facilitate storage and handling. Extract from pigmented rice or its bran,
An arbitrary dosage form such as a powder or a tablet can be obtained by formulation.

The fibroblast-proliferating agent of the present invention can increase the production of interstitial substances such as collagen, fibronectin, and mucopolysaccharide by fibroblasts through fibroblast proliferation. In particular, the agent for promoting fibroblast proliferation of the present invention can exert a beautiful skin effect or a skin aging preventing effect by increasing the amount of collagen produced through proliferation of fibroblasts. Therefore, the fibroblast-proliferating agent of the present invention can be used as a raw material for cosmetic foods and drinks and skin cosmetics.

Pigmented rice or an extract from pigmented rice bran has a unique odor and taste, and the fibroblast-proliferating agent of the present invention is used as a raw material for cosmetics, foods and drinks and skin cosmetics. When it is used, it is not generally blended in a large amount, so that there is no practical problem even if it is used without decolorization, deodorization, etc.

[Cosmetic Foods and Drinks] The cosmetics foods and drinks of the present invention are characterized by containing pigmented rice or an extract from pigmented rice bran. Here, "food and drink for beauty"
Food and drink intended to prevent beautiful skin or skin aging.

[0033] Pigmented rice or an extract from pigmented rice bran can exert a beautifying effect or an antiaging effect on the skin through the proliferation of fibroblasts for synthesizing collagen. It is suitable. The cosmetic food or drink of the present invention contains pigmented rice or an extract from pigmented rice bran,
It may be added to any food or drink that does not hinder its physiological activity, or may be food or drink containing pigmented rice or an extract from pigmented rice bran as a main component.

When a cosmetic food or drink is produced by blending pigmented rice or an extract from pigmented rice bran, for example, saccharides such as dextrin and starch; proteins such as gelatin, soy protein, and corn protein; Alanine,
Amino acids such as glutamine and isoleucine; polysaccharides such as cellulose and gum arabic; oils and fats such as soybean oil and medium-chain fatty acid triglyceride; Can be formulated into any dosage form.

The pigmented rice or the extract from pigmented rice bran thus formulated can be used as it is as a cosmetic food or drink. Food and beverages can also be manufactured.

The amount of pigmented rice or the extract from pigmented rice bran in the cosmetic food or drink of the present invention is such that the intake of pigmented rice or the extract from pigmented rice bran is about
It is preferable to adjust so as to be about 10 to 1000 mg.

Foods and drinks to which pigmented rice or an extract from pigmented rice bran can be added are not particularly limited, and specific examples thereof include soft drinks, carbonated drinks, nutritional drinks, fruit drinks,
Beverages such as lactic acid drinks (including concentrates and powders for preparation of these drinks); ice cream, ice sorbet, shaved ice and other frozen desserts; soba, udon, harusame, gyoza rind, shumai rind, Chinese noodles, instant noodles, etc. Noodles; sweets such as candy, candy, gum, chocolate, snacks, biscuits, jellies, jams, creams, baked goods, etc .; fish and livestock processed foods such as kamaboko, ham, sausage; dairy products such as processed milk and fermented milk ; Salad oil, tempura oil,
Fats and oils and fats and processed foods such as margarine, mayonnaise, shortening, whipped cream and dressing; seasonings such as sauces and sauces; various forms of health and nutritional supplements such as tablets and granules; other soups, stews and salads , Side dishes, pickles, and the like.

[Skin Cosmetic] The skin cosmetic of the present invention is characterized by containing pigmented rice or an extract from pigmented rice bran. Pigmented rice or an extract from pigmented rice bran can exhibit a beautiful skin effect or an antiaging effect on the skin through the proliferation of fibroblasts that synthesize collagen, and is therefore suitable for use in skin cosmetics. That is, by absorbing pigmented rice or an extract from pigmented rice bran from the skin, it can be used to prevent beautiful skin or skin aging.

Skin cosmetics to which pigmented rice or an extract from pigmented rice bran can be added are not particularly limited, and specific examples thereof include ointments, creams, emulsions, lotions, packs and the like.

The amount of pigmented rice or the extract from pigmented rice bran in the skin cosmetic of the present invention can be appropriately adjusted depending on the type of skin cosmetic, the physiological activity of the extract, and the like. The proper blending ratio is about 0.001 to 10.0% by weight.

The skin cosmetic of the present invention may contain various main ingredients and auxiliaries usually used in the manufacture of skin cosmetics, as long as it does not hinder the fibroblast proliferation action. The extract from pigmented rice bran alone is not limited to the main agent.

In the skin cosmetic composition of the present invention, examples of usable ingredients together with pigmented rice or an extract from pigmented rice bran include glycerin, collagen, hyaluronic acid and salts thereof, chondroitic acid and Moisturizing agents such as salts thereof, chitin and chitosan; ultraviolet absorbers such as amyl paradimethylaminobenzoate; complex lipids such as glycerophospholipids and sphingolipids; β-carotene, oil-soluble licorice extract, lycochalcone A, baicalin,
Baicalein and other substances having an active oxygen scavenging effect;
Azulene, glycyrrhizic acid and its salts, glycyrrhetic acid and its derivatives, anti-inflammatory substances such as zinc oxide; riboflavin, tocopherol, ascorbic acid,
Vitamins such as folic acid and derivatives thereof; oily components such as jojoba oil, lanolin, liquid paraffin, squalane, isostearyl alcohol; surfactants such as sodium stearyl sulfate, diethanolamine cetyl sulfate and glyceryl stearate; oxidation of sodium erythorbate and the like Preservatives such as ethyl paraben; cholesterols such as oak extract, chamomile extract, liquorice root extract, rosemary extract, marronnier extract; plant sterols; lipoproteins; bifidobacterium culture, lactic acid bacteria Microorganism-derived components such as cultures, yeast extracts, and liquorice extracts; algal extracts such as brown algae extracts and red algae extracts; γ-
Blood circulation promoters such as oryzanol; antiseborrheic agents such as sulfur; fragrances; alcohols; thickeners such as carboxy polymers; titanium yellow, safflower and other coloring agents.

The fibroblast-proliferating agent, cosmetics, food and drink, and skin cosmetics of the present invention described above are suitably applied to humans, but exhibit the effects of the present invention. As long as it is applicable to animals other than humans.

[0044]

EXAMPLES The present invention will be described in more detail with reference to the following examples, but the scope of the present invention is not limited to these examples.

Production Example 1 As a raw material for extraction, 300 g of brown rice of pigmented rice was immersed in 900 ml of an extraction solvent at 60 ° C. for 1 hour to extract soluble components. This extraction treatment was performed twice, and the obtained extract was concentrated to dryness to obtain an extract of pigmented rice. In the extraction process, two kinds of purple rice (Southern purple from Guangdong, China and morning purple from Akita), black rice (black dominant from Nagano), red rice (Kyushu), red rice (China) Yunnan), and water, 70% ethanol, and ethanol were used as extraction solvents. 70% ethanol is a mixture of water and ethanol at a mixing ratio of 3: 7 (weight ratio).
The yield (% by weight) of the extract obtained using each extraction raw material and each extraction solvent was as shown in Table 1 below.

[Table 1] Rice water extraction 70% ethanol extraction Ethanol extraction Huanan 8.8 2.6 2.8 Asahi 8.9 3.1 2.8 Black dominant 8.9 3.2 3.3 Red rice 9.2 3.5 3.4 Red rice 8.7 3.3 3.3

[Production Example 2] Extraction treatment was carried out in the same manner as in Production Example 1, using the pigmented rice and brown rice bran of white rice used in Production Example 1 as extraction raw materials. At that time, 500 ml of extraction solvent was used for 100 g of bran. The yield (% by weight) of the extract obtained using each extraction raw material and each extraction solvent was as shown in Table 2 below.

[Table 2] Rice water extraction 70% ethanol extraction Ethanol extraction Huanan purple 18.9 15.4 13.6 Asahi 17.1 13.8 14.2 Black dominant 16.7 14.6 13.8 Red rice 22.5 18.1 19.7 Red rice 21.2 17.9 20.6

[Test Example 1] For the pigmented rice extract obtained in Production Example 1, the effect of fibroblast proliferation was evaluated by Tada et al. (H. Tada et. Al., J. Immunol. Methods 93, 157, 1986). of
Tested using the MTT method. The test method is as follows.

(1) Human normal neonatal skin fibroblasts (NB
1RGB) 1 × 10 ⁶ MEM-α-Medium containing 10% FBS
(GIBCO) In a 25 cm ² flask containing a culture solution (pH 7.2), the cells were precultured at 37 ° C under 5% carbon dioxide for 4 days. After the pre-culture, the cells were collected by trypsin treatment, dispensed at 7 × 10 ³ cells / 100 μl / well into a 96-well microplate using a MEM-α-Medium culture solution containing 5% FBS, and the mixture was placed at 37 ° C. Cultured overnight under 5% carbon dioxide.

(2) The 70% ethanol extract of morning purple (sample a) or the 70% ethanol extract of southern purple purple obtained in Production Example 1 was added to the MEM-α-Medium culture solution containing 5% FBS. Sample b) was prepared at various concentrations (400 ppm, 200 ppm, 100 ppm, 50 ppm, 2
(5 ppm) and added to each well of the microplate obtained in the above (1) in an amount of 100 μl each, followed by culturing at 37 ° C. under 5% carbon dioxide for 3 days. The above culture solution from each well
After removing 100 μl each, add MTT reagent (3- (4,5-Di
methyl-2-thiazolyl) -2,5-diphenyl-2H tetrazolium br
omide) (5 mg / ml) (5 mg / ml), add 4.5 μl
Incubated for hours. After incubation,
Add 100 μl of 0.01N hydrochloric acid solution in which 10% SDS (sodium lauryl sulfate) (manufactured by Wako Pure Chemical) is dissolved, add
Incubated overnight at 7 ° C. After the incubation, the absorbance at 570 nm was measured using a microplate reader with the absorbance at 650 nm as a control. Then, by subtracting the absorbance at 650 nm from the absorbance at 570 nm, the absorbance at the time of the sample reaction (570 nm-650 nm) was obtained for sample a and sample b.

(3) To each well of the microplate obtained in (1) above, the same operation as in (2) above was performed without adding the MTT reagent, and the absorbance at 570 nm was measured using the absorbance at 650 nm as a control. did. And 570
By subtracting the absorbance at 650 nm from the absorbance at nm, the absorbance at the time of no reaction of the sample (570 nm-650 nm) was obtained.

(4) To each well of the microplate obtained in the above (1), the same operation as in the above (2) was performed without adding the sample a and the sample b, and the absorbance at 650 nm was used as a control at 570 nm. The absorbance was measured. Then, by subtracting the absorbance at 650 nm from the absorbance at 570 nm, the absorbance at the time of the control reaction (570 nm-650 n
m).

(5) To each well of the microplate obtained in the above (1), the same operation as in the above (2) was carried out without adding the sample a, the sample b and the MTT reagent, to obtain 650 nm
The absorbance at 570 nm was measured using the absorbance at 570 nm as a control. Then, by subtracting the absorbance at 650 nm from the absorbance at 570 nm,
(570 nm-650 nm).

(6) Based on the following equation, the increase rate (%) of fibroblasts was determined for samples a and b, and the proliferative effect of samples a and b on fibroblasts was evaluated.

[0056]

[Equation 1] Increase rate (%) = [{(AC)-(BD)}
/ (BD)] x 100

In the above formula, A is the absorbance at the time of the sample reaction.
(570 nm-650 nm) and B is the absorbance (570 nm-650 n
m), C represents the absorbance when the sample did not react (570 nm-650 nm), and D represents the absorbance when the control did not react (570 nm-650 nm).

The increase rate (%) of the fibroblasts of the samples a and b was as shown in Table 3 below. In Table 3, the rate of increase (%) of fibroblasts was expressed as mean ± SD (n =
7). In Table 3, “ ^** ” indicates p <0.000
1, " ^* " means p <0.05.

[Table 3] Extract concentration (ppm) Sample a Sample b 400 4.1 ± 13.3 11.0 ^* ± 3.1 200 16.9 ^* ± 15.1 7.4 ^* ± 3.6 100 24.1 ^* ± 16.2 6.0 ^* ± 4.1 50 36.2 ^** ± 15.1- 4.8 ^* ± 6.7 25 42.2 ^** ± 14.1 -16.0 ^** ± 7.0

As shown in Table 3, both sample a and sample b showed a proliferative effect on fibroblasts. This indicated that the extract of pigmented rice from brown rice had a fibroblast proliferation effect.

[Compounding Example 1] Using the morning violet water extract obtained in Production Example 1, a soft drink having the following composition was produced by a conventional method. Liquid sugar 12.0g Lemon juice 1.0g Cyclodextrin 0.01g Lemon flavor 0.05g Asahi purple water extract (Production example 1) 0.1g Purified water Remainder (total amount is 100g)

Formulation Example 2 Using the morning purple ethanol extract obtained in Production Example 1, a chewing gum having the following composition was produced by a conventional method. Gum base 20g sugar 55g fructose 15g starch syrup 20g fragrance 0.5g morning purple ethanol extract (Production Example 1) 0.5g

[Compounding Example 3]
Using a 70% ethanol extract, tablets having the following composition were produced by a conventional method. 70% ethanol extract of Huanan purple (Preparation example 1) 50mg Powdered sugar (sucrose) 188mg Glycerin fatty acid ester 12mg

[Formulation Example 4] Using the water extract of southern purple obtained in Production Example 1, granules having the following composition were produced by a conventional method. Southern purple water extract 34mg Beet oligosaccharide 1000mg Indigestible dextrin 289mg Vitamin C 167mg Stevia extract 10mg

[Formulation Example 5] Using the morning violet ethanol extract obtained in Production Example 1 and the morning violet ethanol extract obtained in Production Example 2, an emulsion having the following composition was produced by a conventional method. . Jojoba oil 4.0 g Olive oil 2.0 g Squalane 2.0 g Cetanol 2.0 g Glyceryl monostearate 2.0 g Polyoxyethylene cetyl ether (20E.0) 2.5 g Polyoxyethylene sorbitan oleate (20E.0) 2.0 g 1,3-butylene Glycol 3.0 g Methyl parahydroxybenzoate 0.15 g Flavor 0.05 g Asahi purple ethanol extract (Production example 1) 0.1 g Asahi purple ethanol extract (Production example 2) 1.0 g Purified water balance (total amount is 100 g)

[Formulation Example 6] A lotion having the following composition was prepared by a conventional method using the water extract of Nanpura purple obtained in Production Example 1 and the water extract of Nanpura obtained in Production Example 2. did. Glycerin 3.0g 1,3-butylene glycol 3.0g Polyoxyethylene sorbitan oleate (20E.0) 0.5g Methyl parahydroxybenzoate 0.15g Citric acid 0.1g Sodium citrate 1.0g Fragrance 0.05g Water extract of Southern purple Example 1) 0.2 g South China purple water extract (Production Example 2) 2.0 g Purified water balance (total amount is 100 g)

[Compounding Example 7] A cream having the following composition was prepared by a conventional method using the morning purple ethanol extract obtained in Production Example 1 and the southern purple ethanol extract obtained in Production Example 2. . Liquid paraffin 5.0 g Salamander wax 4.0 g Cetanol 3.0 g Squalane 10.0 g Lanolin 2.0 g Stearic acid 1.0 g Polyoxyethylene sorbitan oleate (20E.0) 1.5 g Glyceryl monostearate 3.0 g 1,3-butylene glycol 6.0 g Paraoxybenzoate Methyl citrate 1.5 g Flavor 0.1 g Asahi purple ethanol extract (Production example 1) 0.1 g Southern violet ethanol extract (Production example 2) 1.0 g Purified water Remainder (total amount is 100 g)

[Formulation Example 8] A pack having the following composition was produced by a conventional method using the morning purple ethanol extract obtained in Production Example 2. Polyvinyl alcohol 15 g Polyethylene glycol 3 g Propylene glycol 7 g Ethanol 10 g Ethyl parahydroxybenzoate 0.05 g Fragrance 0.05 g Asahi purple ethanol extract (Production Example 2) 5 g Purified water The remainder (total amount is 100 g)

[Compounding Example 9]
Using a 70% ethanol extract, a mixture having the following composition was prepared, and the mixture was formed into granules to produce health and nutritional supplements. 70% ethanol extract of Huanan purple (Preparation example 1) 30 parts by weight Lotus embryo extract 200 parts by weight Pearl protein hydrolyzate (including various amino acids and peptides) 15 parts by weight Silk protein hydrolyzate (various amino acids and peptides 15 parts by weight Soy isoflavone 10 parts by weight Red wine extract powder (including procyanidins) 30 parts by weight Yeast extract (including glutathione, nucleic acids, amino acids and vitamins) 10 parts by weight Pomegranate seed extract 10 parts by weight Wheat germ extract (vitamin) , Chromium, selenium, molybdenum) 10 parts by weight DHA 30 parts by weight Beet oligosaccharide 1060 parts by weight Stevia extract 10 parts by weight

[Formulation Example 10] Using the morning violet water extract obtained in Production Example 1, a mixture having the following composition was prepared, and the mixture was gelatin-encapsulated to produce a tablet-shaped health and nutritional supplement. did. Morning purple water extract (Production Example 1) 25 parts by weight Lotus germ extract 20 parts by weight Ceramide 25 parts by weight Phospholipid (lecithin) 10 parts by weight Vitamin E (tocopherol) 17 parts by weight Multi-carotene (α and β carotene, lutein) , Lycopene) 10 parts by weight Octacosanol 1 part by weight Plant sterol 5 parts by weight Perilla oil (including α-linolenic acid) 20 parts by weight Refined fish oil (including DHA and EPA) 20 parts by weight Sesame oil (including lignan compound) 20 parts by weight Parts Olive oil 10 parts by weight Soybean / rapeseed mixed oil 62 parts by weight Glycerin fatty acid ester 12 parts by weight

Formulation Example 11 Using the black-dominated 70% ethanol extract obtained in Production Example 1, a soft drink having the following composition was produced by a conventional method. Black dominant 70% ethanol extract (Production Example 1) 1 part by weight Lotus germ extract 1 part by weight Royal jelly 3 parts by weight Water-soluble collagen 8 parts by weight Pearl barley extract 1 part by weight Korean ginseng extract (including saponin) 1 part by weight Placenta (placenta) extract 1 part by weight Pueraria extract (including flavonoids) 1 part by weight Purple yam extract 1 part by weight Oligosaccharide 5 parts by weight Sucrose 10 parts by weight Prune juice 2 parts by weight Pomegranate juice 5 parts by weight Grapefruit juice 10 parts by weight Vitamin C 1 part by weight Grapefruit flavor 0.7 parts by weight Water Remaining (total amount is 100 parts by weight)

[Formulation Example 12] Using the ethanol extract of red rice obtained in Production Example 1, a candy having the following composition was produced by a conventional method. Red rice ethanol extract (Production Example 1) 5 parts by weight Lotus germ extract 2 parts by weight Mizuna 30 parts by weight Licorice extract 3 parts by weight Tengcha extract 1 part by weight Blueberry extract (including anthocyanin) 1 part by weight Green tea extract (vegetable polyphenol) 3 parts by weight Strawberry 1/5 concentrated juice 1 part by weight Red cabbage pigment 0.05 parts by weight Lemon juice 0.5 parts by weight Water 5 parts by weight

[0073]

Industrial Applicability According to the present invention, a fibroblast-proliferating agent capable of proliferating fibroblasts, a cosmetic food or drink capable of preventing aging of beautiful skin or skin through proliferation of fibroblasts, and A skin cosmetic is provided.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI theme coat ゛ (Reference) A23L 2/38 A61K 7/00 K 4C088 A61K 7/00 N M U 7/48 7/48 A61P 43/00 107 A61P 43/00 107 A23L 2/00 FF term (reference) 4B014 GB06 GG01 GK06 4B017 LC03 LG09 LL09 4B018 MD49 ME10 MF01 4B023 LC09 LE30 LP20 4C083 AA082 AA111 AA112 AA122 AC022 AC072 AC102 AC122 AC042 AD242 AD512 AD642 CC02 CC04 CC05 CC07 DD15 DD16 DD23 DD27 DD31 EE06 EE12 4C088 AB74 AC04 CA03 CA04 CA05 CA06 MA52 MA63 NA14 ZA89 ZB22

Claims (5)

[Claims] 1. A fibroblast-proliferating agent comprising pigmented rice or an extract from pigmented rice bran as an active ingredient. 2. The agent according to claim 1, wherein the extract is an extract obtained using a polar solvent as an extraction solvent. 3. The polar solvent is water, methanol, ethanol, propanol, 1,3-butylene glycol,
3. The agent according to claim 2, which is glycerin, propylene glycol, or a mixture of two or more thereof. 4. A cosmetic food or drink comprising pigmented rice or an extract from pigmented rice bran. 5. A skin cosmetic comprising pigmented rice or an extract from pigmented rice bran.