JP2000266767A - Biochemical analyzer - Google Patents

Abstract

PROBLEM TO BE SOLVED: To simplify the mechanism of a nozzle unit for spot contact and to compact a device, by changing a moving route of a nozzle for spot contact and a conveyance route of spot contact materials, when executing spot contact of two kinds of liquid to a spot contact material by two nozzles for spot contact. SOLUTION: A nozzle unit for spot contact 15 for executing spot contact of two kinds of liquid to a spot contact material 2 has a first nozzle for spot contact 46 for executing spot contact of a first liquid and a second nozzle for spot contact 47 for executing spot contact of a second liquid. Both nozzles for spot contact 46, 47 are moved to a spot contact position F along a linear moving route D, and the spot contact material 2 is conveyed in the direction E crossed by the moving route D of the nozzles for spot contact.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method in which a sample such as blood or urine is spotted on a material to be spotted such as a dry analysis element or an electrolyte slide by a spotting nozzle unit, and a specific component contained in the sample is quantitatively analyzed. More particularly, the present invention relates to a mechanism for spotting two kinds of liquids on a spotted material by a spotting nozzle unit.

[0002]

2. Description of the Related Art In recent years, a dry-type dry analytical element or an electrolyte slide capable of quantitatively analyzing a specific chemical component or a solid component contained in a sample simply by spotting and supplying a small droplet of the sample. (Dry ion selective electrode film) has been developed and put into practical use. The use of dry analytical elements or electrolyte slides makes it easier and faster to analyze specimens than conventional wet analytical methods, so they are particularly useful for medical institutions and research institutions that need to analyze a large number of specimens. It is suitably used in places.

The above-mentioned dry analytical element is disclosed in, for example, Japanese Patent Publication No. 53-21677 (US Pat. No. 3,992,992).
158), JP-A-55-164356 (U.S. Pat. No. 4,292,272), and the like, and an electrolyte slide is disclosed in JP-B-58-4981 (U.S. Pat. No. 4,053,381). Details) are disclosed in the Japanese language.

As methods for quantitative analysis of chemical components and the like in a sample by a biochemical analyzer, two methods are mainly known: a colorimetric method using a dry analytical element and a potential difference measuring method using an electrolyte slide. ing.

[0005] In a biochemical analyzer using a colorimetric method, a sample is spotted on a dry analytical element, which is kept at a constant temperature in an incubator (incubator) for a predetermined time to produce a color reaction (dye formation reaction). Then, irradiating the dry analytical element with measurement irradiation light containing a wavelength selected in advance by a combination of a predetermined biochemical substance in the sample and a reagent contained in the dry analytical element, and measuring its optical density, From the optical density, the concentration of the biochemical substance is determined using a calibration curve representing the correspondence between the optical density determined in advance and the substance concentration of the predetermined biochemical substance.

On the other hand, in a biochemical analyzer using a potentiometric method, instead of measuring the optical density, the biochemical analyzer is contained in a sample spotted on an electrode pair consisting of two pairs of dry ion selective electrodes of the same type. The activity of a specific ion is quantitatively analyzed by potentiometry to determine the substance concentration.

In any of the apparatuses using any of the above methods, a liquid sample is generally stored in a sample container (sampling cup) and set in the apparatus. It is designed to be spotted using a spotting nozzle unit having the spotting nozzle unit. This spotting nozzle fits a disposable nozzle tip at the tip if necessary, aspirates the sample in the sample container, and then moves onto the dry analytical element or the electrolyte slide as the target material. In this way, a predetermined amount is discharged and spotted.

In addition, when it is necessary to dilute a sample such as blood with a diluting liquid, the dropping nozzle sucks and holds the diluting liquid and the sample from the container, and drops them into a mixing cup for mixing the two. Sometimes used for: Further, the spotting nozzle is used for spotting a reference liquid on an electrolyte slide in a biochemical analyzer using the above-described potentiometric method. In this case, a spotting nozzle dedicated to the reference solution spotting is used separately from the sample spotting nozzle.

As described above, the spotting nozzle unit is for dropping two kinds of liquids, ie, a specimen and a reference liquid, on an electrolyte slide as a material to be spotted in the electrolyte measuring method.
In the conventional spotting nozzle unit, a spotting nozzle for spotting the first liquid and a spotting nozzle for spotting the second liquid on the electrolyte slide are provided, and each spotting nozzle is circularly moved by a separate moving mechanism. Spotting was performed by moving along an arc-shaped moving path.

[0010]

However, in the above spotting nozzle unit, a mechanism for moving the spotting nozzle in an arcuate manner to the spotting position is provided for each nozzle. The mechanism and control system are complicated, which leads to an increase in manufacturing costs. In addition, by transporting the pointed material to the intersection of the movement paths of the two-point wearing nozzles, the equipment is compacted to secure these movement paths. It is an obstacle when planning.

In view of the above, the present invention provides a biochemical analyzer which is simplified in structure of a nozzle unit for spotting and which is compact in size by increasing the degree of freedom in transporting a material to be spotted. Is what you do.

[0012]

The biochemical analyzer according to the present invention, which has solved the above-mentioned problems, comprises a spotting nozzle unit for sucking and discharging a liquid, and spotting two types of liquids on a material to be spotted. The spotting nozzle unit has a first spotting nozzle for spotting a first liquid on the workpiece and a second spotting nozzle for spotting a second liquid. The nozzle and the second spotting nozzle move along a linear movement path to respective spotting positions with respect to the spotted material,
The to-be-dressed material is transported in a direction intersecting a movement path of the spotting nozzle.

It is preferable that the first spotting nozzle and the second spotting nozzle are provided so as to move integrally along a linear movement path.

[0014]

According to the present invention as described above, the first spotting nozzle for spotting the first liquid on the workpiece and the second spotting nozzle for spotting the second liquid are provided. Movement of the spotting nozzle by moving the spotting nozzle to the spotting position relative to the pointed workpiece along a linear movement path and transporting the pointed workpiece in a direction intersecting the movement path of the spotting nozzle. This simplifies the mechanism and increases the degree of freedom of the arrangement position of the transfer mechanism for the to-be-dressed material, thereby realizing a compact apparatus.

If the first spotting nozzle and the second spotting nozzle are provided so as to move integrally, the mechanism can be further simplified and the apparatus can be made more compact.

[0016]

Embodiments of the present invention will be described below with reference to the drawings. FIG. 1 is a perspective view showing a schematic mechanism of an example of a biochemical analyzer.

The biochemical analyzer 10 is, as a general measuring system, a storage 1 for storing a cartridge 3 (see FIG. 3) containing a first material to be spotted by a rectangular dry analytical element 1.
1, an incubator 12 for incubating (holding at a constant temperature) the dry analytical element 1 on which a sample is spotted, which is disposed in front of the storage 11, and removes the dry analytical element 1 from the cartridge 3 of the storage 11. An element transporting means 13 for transporting the sample to the incubator 12, a mounting table 14 for mounting and holding samples such as serum and urine, and consumables for biochemical analysis described later, a spotting nozzle unit 15 for spotting, an incubator And measuring means 16 disposed below the measuring means 12. Furthermore, this biochemical analyzer 1
Reference numeral 0 includes a potential difference measuring means 17 for performing a biochemical analysis by a potential difference measuring method using a second material to be spotted on the electrolyte slide 2 (see FIG. 4) as an electrolyte measuring system.

The element transporting means 1 is located near the storage 11.
3, a suction cup 60 for taking out the dry analytical element 1 from the cartridge 3 in the storage 11 by suction and holding.
(Suction cup) is installed.

As shown in FIG. 3, the dry analytical element 1 is formed by coating or bonding a reagent layer on a light-transmitting support made of a plastic sheet such as an organic polymer sheet such as polyethylene terephthalate (PET) or polystyrene. This is a dry analysis film piece (chip) obtained by laminating a spreading layer on this by a laminating method or the like, and does not have a mount equivalent to a mount in many conventional chemical analysis slides.

The reagent layer contains a hydrophilic polymer binder such as gelatin or a porous layer containing a detection reagent (chemical analysis reagent or immunoassay reagent) selectively reacting with an analyte and reagent components necessary for a color reaction. At least one layer. Further, the spreading layer is made of a material that is resistant to friction between the outside and the outside, such as a woven or knitted fabric made of synthetic fibers such as polyester, a woven fabric, a knitted fabric, a nonwoven fabric, or the like made of a blend of natural fibers and synthetic fibers. And functions as a protective layer, and spreads the sample spotted on the spreading layer uniformly on the reagent layer.

The dry analysis elements 1 are stored in the cartridge 3 in a stacked state for each measurement item. The cartridge 3 is composed of a rectangular box-shaped divided box 31.
A first opening 3a through which only one dry analytical element 1 can pass is formed near the lowermost part of one side surface, and a substantially U-shape on the bottom surface into which a suction cup 60 for taking out and holding the element 1 enters. A second opening 3b of the mold is formed.

Further, a vertical rib 3c is projected from a side surface of the box body 31 where the first opening 3a is formed and a side surface opposite to the first opening portion 3a. The cartridges 3 are formed in different lengths, thereby locking and holding the cartridge 3 in the cartridge storage portion of the storage 11 and preventing erroneous insertion of the cartridge 3. In addition, a data recording section 32 is provided on the side surface of the box body 31 with a barcode representing data and the like regarding the stored dry analytical element 1.

The cartridge 3 is loaded in a double-ringed cartridge storage section of the storage 11. The cartridge 3 is stored in the storage 11 at a predetermined humidity (low humidity, dry state).

Although not shown in detail, the storage 11 is provided with a cartridge storage section on a rotating disk-shaped frame, and the frame is rotated by a case body forming the bottom wall and the peripheral wall of the storage 11. It is supported as much as possible. The upper surface of the case body is closed by a lid, and the lid is
The cartridge insertion opening for insertion and ejection of the cartridge is opened at a position corresponding to the two rows of inner and outer cartridge storage sections.
The frame is rotationally controlled by a frame motor, and is controlled so that any one of the cartridge storage sections stops at a position corresponding to the cartridge loading port. The cartridge loading port is selectively opened and closed by a disk-shaped shutter. An element outlet is formed on the bottom surface of the case body in the same manner as the cartridge loading port on the upper surface,
An opening / closing shutter that opens when taking out a predetermined dry analytical element 1 from each cartridge 3 is provided at the element outlet,
The lowermost dry analytical element 1 of the cartridge 3 is taken out to the outside by the take-out suction cup 60 in the element conveying means 13 inserted through the shutter.

As shown in FIG. 3, the take-out suction cup 60 holds the lower surface of the dry analytical element 1 by suction upward, and can be moved vertically and horizontally by driving means (not shown). I have. The take-out suction cup 60 rises at a position below the element take-out opening of the storage 11, comes into close contact with the lowermost dry analysis element 1 from the second opening 3 b at the lower end of the cartridge 3, and sucks this under reduced pressure. Then, the dry analytical element 1 that has been adsorbed is pulled down slightly, and is slid toward the center in a curved shape in the first position of the cartridge 3.
From the opening 3a.

In the biochemical analyzer 10 shown in FIG. 1, the incubator 12 has a disk-shaped main body 70 rotatably supported by a rotation drive mechanism (not shown). Cell 7 for storing
A plurality of cells 1 are arranged at predetermined intervals, and the dry analysis element 1 is heated in this cell 71 at a predetermined temperature (for example, 37 ° C.).
Incubate at

The measuring means 16 has a photometric head 75 for measuring the optical density by the color reaction between the dry analytical element 1 and the sample. Light from a light source 72 is guided to the photometric head 75 via a filter 73 and a mirror 79, and the light is irradiated to the dry analysis element 1 in the photometric head 75. Note that a plurality of types of filters 73 corresponding to the measurement items are installed on the disk 74, and the filter 73 is configured to rotate the disk 74 to select a filter 73 having predetermined characteristics corresponding to the measurement items. ing.

At this time, the light reflected from the dry analytical element 1 carries light information (specifically, the amount of light) corresponding to the amount of the dye generated in the reagent layer of the element 1. Is photoelectrically converted by a photodetector (not shown) of the photometric head 75, and sent to the determination unit via the amplifier. The determination unit determines the optical density of the dye based on the level of the input electric signal, and calculates the concentration (content) or the activity value of a predetermined biochemical substance in the specimen based on the principle of the colorimetric method.

Although not shown in FIG. 1, the main body 70 of the incubator 12 is provided with an element holder for fixing the dry analytical element 1 inserted into the cell 71 at a predetermined position. On the lower surface of the main body 70, photometric windows are opened at predetermined intervals corresponding to the positions where the cells 71 are formed, and a photometric head 75 is provided at a position matching one of these photometric windows.

The element transporting means 13 for transporting the dry analytical element 1 from the storage 11 to the incubator 12 comprises the above-mentioned take-out suction cup 60 and the dry-type analytical element 1 held by the take-out suction cup 60, After receiving and holding the sample from below while the sample is in the state of being on the upper surface and transporting the sample to the spotting position C, the cell 71 of the incubator 12
A substantially horseshoe-shaped transfer member 76 inserted from the side opening into
A holding suction cup (not shown) for holding the dry analytical element 1 held by the transfer member 76 by protruding and retracting from below the cell 71 is provided.

The dry analytical element 1 after the above measurement is taken out of the cell 71 of the incubator 12, transported by the transfer member 76 to the vicinity of the disposal box 77, and disposed by the disposal means 78. Discarded during 77.

On the other hand, the electrolyte slide 2 used for measuring the electrolyte by the potential difference measuring means 17 measures the electrolyte of the specimen by an electric change, and as shown in FIG.
a reference liquid spotting portion 2b, a sample spotting portion 2c, and a bridge 2d connecting the two spotting portions are provided on the upper surface thereof, and three types of multilayer film electrode pairs (Na, K) are provided inside. , C
1 measurement electrode pair) and a distribution member in contact with the electrode.

A large number of the electrolyte slides 2 are stored in the slide storage portion 51 in a stacked manner. When measuring the electrolyte, the slides are transported one by one from the slide storage portion 51 in the transport direction E by the transport mechanism. Will be sent. At this spotting position F, the electrolyte slide 2 in which the sample and the reference liquid are spotted almost simultaneously on the sample spotting part 2c and the reference liquid spotting part 2b.
Is further transported in the transport direction E by the transport mechanism and sent to the measurement unit 52, where the potential difference between the sample and the reference liquid is measured. In addition, as a conveyance mechanism of the said electrolyte slide 2,
For example, a slide transport mechanism disclosed in JP-A-6-66818 can be used.

The transport direction E of the electrolyte slide 2 intersects a linear movement path D of the first and second spotting nozzles 46 and 47 in the spotting nozzle unit 15 described below with respect to the spotting position F. (Actually orthogonal)
Direction.

On the mounting table 14, a sample rack 21 for holding a plurality of sample containers 20 each containing a liquid sample, a dilution plate 23 having a plurality of small cup-shaped recesses 22, and a sample diluent are stored. As an example, five diluting liquid containers 24, one reference liquid container 25 containing a reference liquid for measuring an electrolyte, and a tip rack 27 holding many nozzle tips 26 in an upright state are mounted and held. Has become. The mounting table 14 is movable in a front-rear direction (arrow B direction) by a driving unit (not shown) in a direction approaching and leaving the spotting nozzle unit 15.

On the other hand, the spotting nozzle unit 15 is
As shown in (A), two horizontal guide rods 4
0, and the horizontal guide rod 40 is parallel to a straight line D (moving path of spotting nozzles 46 and 47 to be described later) connecting the spotting position C of the dry analytical element 1 and the spotting position F of the electrolyte slide 2. It is arranged in. This horizontal guide rod 4
A horizontal movement block 41 is supported at 0 and is horizontally movable. A pair of first vertical guide rods 42 extending downward from the horizontal movement block 41 and a similar pair of second vertical guide rods 43 are provided. It is established.

At the tip of the first vertical moving block 44 supported by the first vertical guide rod 42 and vertically moved, a first spotting nozzle 46 for sample spotting is attached. A second spotting nozzle 47 for spotting a reference liquid is attached to the tip of a second vertical movement block 45 supported by the second vertical guide rod 43 and vertically moved. These first and second spotting nozzles 46, 4
7 is provided on the straight line D.

The horizontal moving block 41 is fixed to a part of the first belt 53 disposed in the horizontal direction,
3 is driven by the first motor 54, and the lateral movement of the lateral movement block 41 is drive-controlled in accordance with the traveling of the first motor 54, whereby the first and second spotting nozzles 46, 47 are integrally united in the spotting positions C, The horizontal movement path D connecting F is moved.

FIG. 2B shows another embodiment.
As the first and second spotting nozzles 46 and 47 move integrally and linearly, the linear lateral movement path D of the first spotting nozzle 46 is the same as that of FIG. While passing through the landing position C, the horizontal movement path D ′ of the second spotting nozzle 47 is shifted from the movement path D in a direction orthogonal to the movement direction of the horizontal movement block 41. At this time, the transport direction E of the electrolyte slide 2 intersects the lateral movement paths D and D ′, and the electrolyte slide 2 stopped at the spotting position F.
The sample spotting section 2c is located on the horizontal movement path D of the first spotting nozzle 46, and the reference liquid spotting section 2b is located on the horizontal movement path D 'of the second spotting nozzle 47.

The first vertical moving block 44 is fixed to a part of a second belt 55 disposed in the vertical direction, and the belt 55 is driven by a second motor 56.
The vertical movement of the first vertical movement block 44 is drive-controlled according to the traveling, whereby the first spotting nozzle 46 independently moves vertically. Similarly, the second vertical moving block 45 is fixed to a part of a third belt 57 disposed in the vertical direction, and the belt 57 is driven by a third motor 58, and the second vertical The vertical movement of the moving block 45 is drive-controlled, whereby the second spot nozzle 4
7 moves up and down independently.

The first spotting nozzle 46 is formed in the shape of a rod, and an air passage extending in the axial direction is provided therein. The pipetting nozzle tip 26 is fitted in the tip of the spotting nozzle 46 in a sealed state. Are combined. One end of a hollow air tube 48 is connected to the first spotting nozzle 46,
The other end of the air tube 48 is connected to suction / discharge means 49 for supplying suction / discharge pressure. This suction and discharge means 4
Reference numeral 9 denotes a syringe pump or the like, and its operation controls the suction and discharge of the liquid from the nozzle tip 26. Although not shown in FIG. 1, the second spotting nozzle 4
Reference numeral 7 is also connected to the same suction and discharge means, and the suction and discharge of the liquid are drive-controlled.

Hereinafter, measurement by the biochemical analyzer 10 having the above configuration will be described. First, as a preparation operation, a sample measurement item is designated from a keyboard connected to the apparatus control unit, and a sample container 2 containing a sample is designated.
0 is set on the sample rack 21 of the mounting table 14, and the dilution plate 23, the diluting liquid container 24, the reference liquid container 25, and the chip rack 27 are set on the mounting table 14. This setting operation is performed at the standby position shown in FIG. 1 where the mounting table 14 has moved backward.

When the start of measurement is instructed, in the case of the general measurement system, the suction cup 60 for removal of the element transport means 13 operates as described above, and moves to a position corresponding to the element outlet on the bottom surface of the storage 11. Then, the dry analytical element 1 is taken out of the cartridge 3 corresponding to the measurement item. This suction cup 6
The dry analytical element 1 held at 0 is transferred to the transfer member 76 and moved to the spotting position C, where the diluted sample is spotted.

This spotting is performed as follows.
First, the mounting table 14 moves forward from the standby position, the tip rack 27 moves below the horizontal movement path D of the spotting nozzles 46 and 47, and the tip of the first spotting nozzle 46 by the operation of the spotting nozzle unit 15. One nozzle tip 26 in the tip rack 27 is attached to the nozzle rack 27.

Next, the first spotting nozzle 46 is mounted on the mounting table 14.
Sample container 20 containing the sample to be measured along with the movement of
And the tip of the nozzle tip 26 is moved to the sample container 20.
A predetermined amount of the sample is sucked and held in the nozzle tip 26 by the suction operation of the suction / discharge means 49. A number is assigned to each sample container storage unit of the sample rack 21, and the number of the storage unit in which the sample container 20 storing the sample to be measured is set in advance to the device control unit together with the individual data of the sample, The movement of the mounting table 14 is controlled.

Next, the first spotting nozzle 46 is moved above one recess 22 of the dilution plate 23, and the nozzle tip 26 is lowered to a position close to the recess 22. Next, after the sample held in the nozzle tip 26 is dropped into the concave portion 22 by the discharge operation of the suction / discharge means 49, the nozzle tip 26 is removed from the tip of the first spotting nozzle 46 by a known means.

Next, a new nozzle tip 26 in the tip rack 27 is attached to the tip of the first spotting nozzle 46 by the same operation as described above, and the diluent in the diluent container 24 is Is aspirated by a predetermined amount, and is dropped into the concave portion 22. Thereafter, in the concave portion 22 of the dilution plate 23, the suction and discharge of the liquid by the first spotting nozzle 46 are alternately and repeatedly performed, so that the sample and the diluent are uniformly mixed. Next, the first spot wearing nozzle 4
By the suction operation of No. 6, a predetermined amount of the uniformly mixed diluted sample is sucked into the nozzle tip 26.

Next, the first spotting nozzle 46 is moved to a position above the dry analytical element 1 held at the spotting position C by the element transporting means 13, and the first spotting nozzle 46 discharges the nozzle. The diluted sample in the nozzle tip 26 is spotted on the reagent layer of the dry analysis element 1. The dry analytical element 1 onto which the diluted sample has been spotted is sent to the cell 71 of the incubator 12, where it is incubated and measured for optical density. The concentration (content) or activity value of a predetermined biochemical substance in the sample determined based on this optical density is
It is displayed on a display unit (not shown).

When the sample is spotted on the dry analytical element 1 by the first spotting nozzle 46, the second spotting nozzle 47 moves sideways as a unit, but is operated to the ascending position to operate the first spotting nozzle 47.
The spotting nozzle 46 does not hinder the spotting operation.

Next, when measuring the electrolyte of the sample,
The sample is spotted on the sample spotting part 2c of the electrolyte slide 2 and the reference liquid is spotted on the reference liquid spotting part 2b almost simultaneously.

The spotting of the sample is carried out by using the first spotting nozzle 46 in common.
The mounting of the sample 6 and the suction holding of the sample are performed basically in the same manner as described above. First, the sample is suctioned and held in the nozzle tip 26 of the first spotting nozzle 46. On the other hand, the reference liquid is spotted using the second spotting nozzle 47,
Of the reference liquid container 25 is opened, and the nozzle tip 2
6 is attached to the reference liquid container 2
5, and a predetermined amount of the reference liquid is sucked and held in the nozzle tip 26.

Then, the first spotting nozzle 46 holding the sample and the second spotting nozzle 47 holding the reference liquid integrally move in a linear movement path D (or D and D) with the horizontal movement of the horizontal movement block 41. D ′) is moved to the spotting position F of the potential difference measuring means 17 and the moving path D (or D
And D ′), are moved downward at the position above the electrolyte slide 2 conveyed in the conveyance direction E intersecting with the sample, and apply the sample to the sample application section 2c and the reference liquid to the reference liquid application section 2b.

The electrolyte slide 2 on which the sample and the reference solution are spotted in this way is sent to the measuring section 52, and the potential difference between the sample and the reference solution is measured. Then, based on this potential difference, the activity of the specific ion contained in the sample is quantitatively analyzed by potentiometry, and the concentration of the specific substance is obtained.

As described above, this biochemical analyzer 10
First spotting nozzle 4 for spotting a sample on electrolyte slide 2
6 and a second spotting nozzle 47 for spotting the reference liquid, a linear moving path D or D is integrally formed between the mounting table 14, the spotting position C of the dry analytical element 1, and the spotting position F of the electrolyte slide 2. D ′, and independently move up and down, and the electrolyte slide 2 is transported in the transport direction E intersecting the travel path D or D and D ′ to perform spotting. As compared with the conventional device for moving the nozzle, the mechanism of the spotting nozzle unit 15 can be simplified and downsized, and the degree of freedom of the installation position of the potential difference measuring means 17 can be increased, so that the downsizing can be further realized and the manufacturing cost can be reduced. .

[Brief description of the drawings]

FIG. 1 is a perspective view showing a schematic configuration of a biochemical analyzer according to one embodiment of the present invention.

FIG. 2A is a perspective view of a main part schematically showing a driving mechanism of a spotting nozzle unit, and FIG. 2B is a perspective view of a main part in another embodiment.

FIG. 3 is a perspective view showing a state in which a dry analytical element is removed from a cartridge together with a suction cup for removal.

FIG. 4 is a perspective view of an electrolyte slide.

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 1 Dry-type analytical element (pointing material) 2 Electrolyte slide (pointing material) 3 Cartridge 10 Biochemical analyzer 11 Storage 12 Incubator 13 Element transport means 14 Mounting table 15 Point wearing nozzle unit 16 Measuring means 17 Potential difference measuring means 20 Sample container 24 Diluent container 25 Reference solution container 26 Nozzle tip 41 Lateral moving block 44,45 Vertical moving block 46,47 Pointing nozzle 49 Suction / discharge means C, F Spotting position D, D 'Moving path E Transport direction

Continued on the front page F-term (reference) 2G042 BA10 BC01 CA10 CB03 DA08 EA20 FA11 FB05 FB07 FC02 FC07 GA01 HA03 2G045 AA01 AA15 BA20 BB41 DB09 DB10 DB16 FA14 FB03 FB05 FB11 FB13 FB15 FB16 FB19 GC12 JA0 HA09 HA09 BB03 BB07 BB09 BB15 CB15 CC08 CC09 CD04 CD12 CF16 CF28 EA11 EA12 EB02 ED02 ED18 ED19 ED35 FA04 GA02 GA11 GC05 GC06 HA01

Claims (2)

[Claims] 1. A biochemical analyzer comprising a spotting nozzle unit for sucking and discharging a liquid, wherein two types of liquids are spotted on a spotted material. A first spotting nozzle for spotting the first liquid, and a second spotting nozzle for spotting the second liquid, wherein the first spotting nozzle and the second spotting nozzle are the spotted material A biochemical analyzer that moves along a linear movement path to each of the spotting positions with respect to, and transports the pointed material in a direction intersecting the movement path of the spotting nozzle. 2. The biochemical analyzer according to claim 1, wherein the first spotting nozzle and the second spotting nozzle move integrally.